# Maris Stella High School GCE O Level Biology WS EX 3.

Name: ___________________ Class (Index No.): _____ ( ) Date: ________________

1a) Explain the change in the solution level in the capillary tube of set-up A during the first 20 minutes. Height of solution in tube rises rapidly from 0 to 170 mm in 20 minutes [1/2] This is because solution outside dialysis tubing has a higher water potential compared to the solution inside the tubing; Sucrose molecules are too large to move through the partially permeable tubing [1/2] There is a net movement of water molecules into the tubing via osmosis, down a water potential gradient; This leads to an increase in the volume of solution inside the tubing, resulting in an increase in height; [4]

2008 Ex 3.1

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Maris Stella High School O Level Biology

Diffusion & Osmosis Topic 3

1b) Explain the difference in the rate of change of solution levels between set-ups A and B during the first 20 minutes. [4]

The rise in height for Setup A is much faster (increase of 170 mm) than Setup B (increase of 40 mm) in the first 20 minutes [1/2] This is because in Setup B, there is a net movement of glucose molecules out of the tubing via diffusion down a concentration gradient; This lowers the water potential of the solution outside the tubing and creates a gentler water potential gradient [1/2] The rate of net movement of water molecules into the tubing via osmosis down the water potential gradient would be slower, hence resulting in a smaller/slower rise in the height of solution;

2(a) In which direction will a net movement of water occur? Explain your answer. The solution on the left has a total solute concentration of 0.36 mol/dm3 while the solution on the right has a total concentration of 1.11 mol/dm3; This means that the solution on the left has a higher water potential [1/2] There would be a net movement of water molecules from the left to the right via osmosis; Down a water potential gradient;

[3]

(b) What other molecules will cross the membrane and in what direction will they move? Explain your answer. As this is a monosaccharide permeable membrane, only glucose and fructose molecules are able to pass through, while maltose molecules would be too large to pass through the membrane; There is a higher concentration of glucose molecules on the right (0.7 compared to 0.3 mol/dm3) [1/2] There would be a net movement of glucose molecules from the right to left via diffusion down a concentration gradient; There is a higher concentration of fructose on the left (0.02 compared to 0.01 mol/dm3) [1/2] There would be a net movement of fructose molecules from the left to the right via diffusion down a concentration gradient;

[4]

2008 Ex 3.1

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