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PromoCell GmbH

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2013 | 2014

Cell Culture & Cell Biology

2013 | 2014

Cell Culture & Cell Biology

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Human Primary Cells & Media


Stem and Blood Cells & Media
Cell Biology Products

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PromoCell catalog

PromoCell

PromoKine catalog

PromoKine

PromoCell Academy catalog

PromoCell Academy

Date, Signature

Overview

About PromoCell

Human Primary Cells

Human Stem and Blood Cells

Media and Sera

Cell Pellets

Reagents and Supplements

Cell Analysis

Apoptosis

Fluorescent Labeling

Cell Transfection

10

Antibodies and ELISAs

11

Cytokines and Growth Factors

12

Microbial Detection and Elimination

Contents

Overview

Contents

Contents

Contents

0
1

About PromoCell

PromoCell Ethical Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6


Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
PromoCells Knowledge Base . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Human Primary Cells


1.1 Cardiac Myocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.2 Chondrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.3 Endothelial Cells (Large Vessels) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.4 Endothelial Cells (Microvascular) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.5 Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.6 Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.7 Follicle Dermal Papilla Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.8 Hepatocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.9 Keratinocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.10 Melanocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.11 Osteoblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.12 Preadipocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.13 Skeletal Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.14 Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

11
12
13
17
23
27
30
31
32
33
34
35
36
37

Overview Human Primary Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42


Human Primary Cells by Tissue Type . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Human Stem and Blood Cells


2.1 Mesenchymal Stem Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.2 Pericytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.3 CD34+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.4 CD133+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.5 Monocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.6 Mononuclear Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

49
51
52
53
54
56

Overview Human Stem and Blood Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

3
4

Media and Sera


3.1 Media for Primary Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.2 Media for Stem and Blood Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.3 Classical Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.4 Insect Cell Culture Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.5 Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

61
80
85
87
87

Cell Pellets
4.1 Cell Pellets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

Contents

6
7
8
9

Reagents and Supplements


5.1 Detachment Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.2 Antibiotics and Antimycotics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.3 Buffers and Salt Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.4 Supplements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

95
97
98
99

Cell Analysis
6.1 Cell Viability and Proliferation Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.2 Live/Dead Cell Staining Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.3 Cytotoxicity Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.4 Senescence Detection Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.5 Cell-Based Reporter Assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.6 Cell Staining Reagents and Related Products . . . . . . . . . . . . . . . . . . . . . . .
6.7 Intracellular Indicators and Related Products . . . . . . . . . . . . . . . . . . . . . . . .
6.8 Cell Signaling Assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.9 Cell Stress and Metabolism Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.10 Cell Fractionation and Protein Extraction Kits . . . . . . . . . . . . . . . . . . . . . . .
6.11 Protein Size Markers and Protein Gel Stains . . . . . . . . . . . . . . . . . . . . . . . .
6.12 Nucleic Acid Isolation Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
6.13 DNA Size Markers and DNA Gel Stains . . . . . . . . . . . . . . . . . . . . . . . . . . . .

103
105
106
106
107
109
115
119
120
122
123
124
125

Apoptosis
7.1 DNA Fragmentation Detection Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.2 Mitochondrial Apoptosis Staining Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.3 Cytochrome c Apoptosis Detection Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.4 Glutathione Detection Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.5 Annexin V Detection Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.6 Caspase Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.7 Cathepsin/Calpain Detection Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.8 Kinase Detection Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.9 Apoptotic and Necrotic Cell Detection . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.10 Apoptotic Cell Isolation Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
7.11 Apoptosis Inducers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

129
130
130
131
132
134
138
139
140
141
141

Fluorescent Labeling
8.1
8.2
8.3
8.4
8.5

PromoFluor Dyes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
PromoFluor Labeling Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
PromoFluor Labeled Biomolecules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Phycobiliprotein Dyes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
PromoFluor Antifade Reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

145
148
150
152
153

Cell Transfection
9.1
9.2
9.3
9.4

PromoFectin Transfection Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .


Magnet Assisted Transfection (MATra) . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Transfection Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Reporter, Expression and Fusion Vectors . . . . . . . . . . . . . . . . . . . . . . . . . . .

157
161
165
166

Contents

Contents

Contents

Contents

10

Antibodies and ELISAs

11

Cytokines and Growth Factors

10.1 Mono- and Polyclonal Antibodies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .


10.2 Antigens and Cell Lysates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
10.3 Labeled Antibodies and Labeling Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
10.4 ELISA Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

169
172
173
175

11.1 Cytokines, Growth Factors, Hormones, and soluble Receptors . . . . . . . . . . . 179


11.2 Animal-free Cytokines and Growth Factors . . . . . . . . . . . . . . . . . . . . . . . . . 180
11.3 Cytokines and Growth Factors for Stem Cell Research . . . . . . . . . . . . . . . . 181

12

Microbial Detection and Elimination

13

Appendix

12.1 Mycoplasma Test Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .


12.2 Mycoplasma Elimination Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
12.3 Bacteria Test Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
12.4 Disinfectants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Alphabetical Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
International Distributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Terms and Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Ordering and Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Fax Order Form . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

185
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188
189

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209

About PromoCell
Over 20 years ago PromoCell started its business in the
so-called City of Science: Heidelberg. Heidelberg is not only
home to the oldest university in Germany but is also popular for
research related businesses. The city provides the ideal scientific
environment for PromoCell and serves as initial point for all our
operations.

0 About PromoCell

PromoCell Ethical Standards


Ethical and legal standards
governing Primary Human Cells

About PromoCell

PromoCell is the original manufacturer


of all primary, stem, and blood cells featured in this catalog and is committed to

the highest ethical and legal standards.


Consequently, PromoCell strictly complies with the following procedure for
the donation and collection of human
tissue used for cell isolation:

Extract from The Convention of Human Rights and Biomedicine:


Article 5 General rule
An intervention in the health field may only be carried out after the person
concerned has given free and informed consent to it.
This person shall beforehand be given appropriate information as to the purpose and nature of the intervention as well as on its consequences and risks.
The person concerned may freely withdraw consent at any time.
Article 22 Disposal of a removed part of the human body
When in the course of an intervention any part of a human body is removed,
it may be stored and used for a purpose other than that for which it was
removed, only if this is done in conformity with appropriate information and
consent procedures.

The tissue used by PromoCell for the


isolation of human cell cultures is derived from donors who have signed
an informed consent form (this being
done by the donor himself, an authorized agent, or a legal agent) which
outlines in detail the purpose of the
donation and the procedure for processing the tissue. We do not accept
or use any tissue without prior signing
of the consent documents.
When obtaining and using human
tissue, PromoCell acts in strict compliance with
1. The Convention for Protection
of Human Rights and Dignity of the
Human Being with Regard to the Application of Biology and Medicine:
Convention of Human Rights and
Biomedicine published on April 4th,
1997 by the Council of Europe (European Treaty Series no 164).
2. The Human Tissue Act published
on November 15th, 2004 by the government of the United Kingdom. This
act aims to make consent a fundamental principle underpinning the use
and storage of human tissue.

Extract from The Declaration of Helsinki:


Article 22
In any research on human beings, each potential subject must be adequately
informed of the aims, methods, sources of funding, any possible conflicts of
interest, institutional affiliations of the researcher, the anticipated benefits
and potential risks of the study and the discomfort it may entail. The subject
should be informed of the right to abstain from participation in the study or
to withdraw consent to participate at any time without reprisal. After ensuring
that the subject has understood the information, the physician should then
obtain the subjects freely-given informed consent, preferably in writing. If
the consent cannot be obtained in writing, the non-written consent must be
formally documented and witnessed.

3. The Declaration of Helsinki developed by the World Medical Association as a statement of ethical principles to provide guidance to physicians
and other participants in medical
research involving human subjects.
4. The German Federal Data Protection Act to protect privacy of donors.

Should you have any questions concerning ethical aspects related to the
use of Primary Human Cells, do not
hesitate to contact us or visit our
website:
www.promocell.com/ethics

About PromoCell

0 About PromoCell

At PromoCell we continuously work to improve our products and services.

Dear Customer,
Henry Ford once said: Coming together is a beginning. Keeping
together is progress. Working together is success. At PromoCell
we are proud to work together successfully with scientists worldwide, providing them with high quality products and experienced
technical customer support. With over 5,000 PromoCell and
PromoKine products we have the tools to meet the needs of your
research project!
Under our PromoCell brand we offer a broad range of human
primary cells, stem cells and blood cells, as well as optimized cell
culture media. Scientists worldwide use PromoCell products in basic and applied biomedical research to obtain better results from
more accurate physiological models. The reliability of our cells provides the basis for being able to offer you the high quality standards you need.
For more in depth analysis of your cells in culture, why not look to
our PromoKine brand? PromoKine offers quality kits and reagents
for cell biology including those for cell analysis, cell transfection,
and fluorescent labeling and also provides numerous antibodies,
ELISAs, cytokines and growth factors.
In order to complete our portfolio we established the PromoCell
Academy in 2004. The PromoCell Academy offers courses with
state-of-the-art insight and up to date trends in the Life Sciences
in a professional and hands-on setting. You can choose from a
growing collection of course topics in German as well as in English.
We are looking forward to working with you and providing you
with our support!
Scientists supporting Scientists guaranteed.

Your PromoCell Team

0 About PromoCell

About PromoCell

Discover our Knowledge Base

Need Information?

Information at your fingertips

Sections of the Knowledge Base include:

As scientists, we know that research is


not a nine-to-five job. Scientists need information fast, at any time, day or night.

Technical

To assist you with this, PromoCell has


created our new online Knowledge Base.
At the touch of a button, you can now
find a wealth of product-related and
technical information to support your
research needs.

You can find detailed answers to many


questions about our products in the section Technical Library. Type in a keyword and the Knowledge Base will show
you the associated technical questions
and answers within seconds.

Library
Notes
Reference Literatures
Application

In the section Application Notes you


will find documents which supplement
the information in our Product Manuals
(e.g. detailed differentiation protocols
and staining procedures).
If you are interested in viewing publications by our customers, the section
Reference Literature offers you access
to literary citations of scientists that use
our PromoCell and PromoKine products.

To access the PromoCell Knowledge Base, please


go to: www.promocell.com/knowledge-base

To access the PromoKine Knowledge Base, please


go to: www.promokine.info/knowledge-base

We appreciate your feedback


We are continually improving our products and services to make them fit your
needs. Your feedback, ideas and suggestions therefore are valued and important
to us.
For your convenience we have created
a feedback form online for this purpose:
www.promocell.com/feedback

We thank you in advance for providing


us with your ideas and suggestions.
Of course our customer support team
is also happy to receive your feedback
via email, fax or telephone.
To find your personal area contact,
please refer to the contact section on our
website or see page 205.

ac

db

e
Fe

www.promocell.com/feedback

Human Primary Cells


Experiments with human primary cells simulate in vivo conditions much better and yield more significant results than those
using immortalized cell lines. This is because the primary cells
genome and expression patterns have not been altered by
immortalization and numerous passages in culture.
PromoCell offers a wide variety of high quality human primary
cells from tissue obtained from approved medical centers.

10 Content Chapter 1

Human Primary Cells


1.1

Cardiac Myocytes . . . . . . . . . . . . . . . . . . . . 11

1.2 Chondrocytes . . . . . . . . . . . . . . . . . . . . . . . 12
1.3

Endothelial Cells (Large Vessels) . . . . . . . . . 13

Umbilical Vein . . . . . . . . . . . . . . . . . . . . . . 13

Umbilical Artery . . . . . . . . . . . . . . . . . . . . . 14

Aortic / Coronary Artery . . . . . . . . . . . . . . . 15

Pulmonary Artery / Saphenous Vein . . . . . . 16

1.4

Endothelial Cells (Microvascular) . . . . . . . . 17

Dermal . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Dermal Blood . . . . . . . . . . . . . . . . . . . . . . . 18

Dermal Lymphatic . . . . . . . . . . . . . . . . . . . 19

Bladder / Cardiac . . . . . . . . . . . . . . . . . . . . 20

Pulmonary / Uterine . . . . . . . . . . . . . . . . . 21

Brain . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
1.5

Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . 23

Nasal / Tracheal . . . . . . . . . . . . . . . . . . . . . 23

Bronchial / Small Airway . . . . . . . . . . . . . . . 24

Mammary / Renal . . . . . . . . . . . . . . . . . . . 25

Renal Cortical / Placental . . . . . . . . . . . . . . 26


1.6 Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . 27

Dermal . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Pulmonary / Aortic Adventitial / Cardiac . . 28

Uterine / Villous Mesenchymal . . . . . . . . . . 29

1.7

Follicle Dermal Papilla Cells . . . . . . . . . . . . . 30

1.8 Hepatocytes . . . . . . . . . . . . . . . . . . . . . . . . 31
1.9 Keratinocytes . . . . . . . . . . . . . . . . . . . . . . . 32
1.10 Melanocytes . . . . . . . . . . . . . . . . . . . . . . . . 33
1.11 Osteoblasts . . . . . . . . . . . . . . . . . . . . . . . . 34
1.12 Preadipocytes . . . . . . . . . . . . . . . . . . . . . . . 35
1.13 Skeletal Muscle Cells . . . . . . . . . . . . . . . . . 36
1.14 Smooth Muscle Cells . . . . . . . . . . . . . . . . . 37
Aortic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

Coronary Artery / Pulmonary Artery . . . . . . 38

Umbilical Artery / Tracheal . . . . . . . . . . . . . 39

Bronchial / Bladder . . . . . . . . . . . . . . . . . . . 40

Prostate / Uterine . . . . . . . . . . . . . . . . . . . . 41

Overview Human Primary Cells . . . . . . . . . . . . . . 42


Human Primary Cells by Tissue Type . . . . . . . . . . . 45

1 Human Primary Cells

11

Human Primary Cells


PromoCell offers a wide variety of high
quality human primary cells. The tissue
used for the isolation of these cells is
obtained from approved medical centers following strict ethical standards
(see page 6 for details). Shortly after
isolation, the cells are cryopreserved
using the unique serum-free PromoCell
Cryo-SFM (see page 79) and an op-

timized, computer controlled freezing


profile. This allows high viability of the
cells and defined culture conditions after
thawing.
All PromoCell primary cells are available
cryopreserved or proliferating. Each cell
lot is tested for cell type specific markers,
cell morphology, population doubling
time, and proliferation capacity. In addi-

tion, tests for the absence of HIV 1, HIV 2,


HBV, HCV, fungi, mycoplasma, and other
bacteria are performed.
Moreover, cell pellets prepared from released cell lots and stored in RNAlater
are available. They are useful for DNA,
RNA, and protein isolation and their subsequent analysis (for more information
see page 91).

Applications
Cardiac

research
studies
Pharmacological studies
Physiological

Features
Adult

origin
-actinin positive
Slow muscle myosin positive
Qualified for long-term experiments
Sarcomeric

Human Cardiac Myocytes (HCM) are


isolated from the ventricles of the adult
heart. They are qualified for in vitro
research on cardiac diseases and for
pharmacological studies. Unlike freshly
isolated rod-shaped myocytes, cultured
HCM can be used for long-term experiments like investigating the long-term

effects of cytokines, mechanical strain,


or cell-cell interactions, as they are prepared according to a special protocol.
Initially, the HCM act more like progenitor cells in that they are not yet fully differentiated. They express the markers
of early stage differentiation such as
GATA-4 and sarcomeric alpha-actin and
have a high capacity for proliferation.
When they are grown to confluency
and cultivated for an extended period of
time, the differentiation process begins.
Markers of late differentiation (e.g. sarcomeric alpha-actinin, slow muscle myosin) are increased and the cells begin to
form myotube-like structures.
Recommended media & reagents

Human Primary
Cells

1.1 Cardiac Myocytes

Fig. 1: HCM culture in phase contrast.


Related products
Human Cardiac Microvascular
Endothelial Cells (see page 20)
Human Cardiac Fibroblasts
(see page 28)
HCM Pellet (see page 91)
PromoFectin Transfection Reagent
(see page 157)

Myocyte Growth Medium (see page 62)


DetachKit
Cryo-SFM

(see page 95)


(see page 79)

Count
Sarcomeric
-actinin negative

Sarcomeric
-actinin positive
Cell Lab Quanta SC TM, Beckman Coulter, Inc.

75

50

Fig. 2: Flow cytometric analysis of PromoCell HCM


after being cultured for 60 days under confluent conditions. 96 % of the cells express sarcomeric -actinin, a
marker of late differentiation.

25

0
0

10

102

103

104

Sarcomeric -actinin

https://www.promocell.com/shop
Product
Human Cardiac Myocytes (HCM)
HCM Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12810
C-12811

1x106 cells in RNAlater

C-14080

12

1 Human Primary Cells

1.2 Chondrocytes
Applications
Osteoarthritis

research
research
Physiology studies
Chondrocyte differentiation
experiments
Hyaline cartilage investigations
Human Primary
Cells

Joint

produce and maintain the extracellular


matrix of cartilage, i.e. collagen (mostly
type II) and proteoglycans (primarily aggrecan).

Recommended media & reagents


Growth Medium
(see page 62)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Chondrocyte

Related products
Pellet (see page 91)
PromoFectin Transfection Reagent
(see page 157)
HCH

Features
Available

from hip and knee joints


tested by Alcian Blue
staining of HCH spheroids
Cryopreserved at second passage

Fig. 1: HCH culture in phase contrast.

Human Chondrocytes (HCH) are isolated from normal human articular cartilage
from the knee and hip joints (lot specific
source information is available on request). PromoCell HCH are routinely
tested for their capacity to differentiate
in 3D spheroids (see Fig. 2).
The articular cartilage covers the joints
between bones and contains no blood
vessels, lymphatic vessels, or nerve fibers. It is composed of only one specialized cell type, the chondrocytes, which

Fig. 2: Spheroids of differentiated HCH


cultured in Chondrocyte Growth Medium
and stained with Alcian Blue.

Differentiation

https://www.promocell.com/shop
Product
Human Chondrocytes (HCH)
HCH Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12710
C-12750

1x106 cells in RNAlater

C-14070

1 Human Primary Cells

13

1.3 Endothelial Cells (Large Vessels)


Applications
Angiogenesis

research
research

Atherosclerosis
Oncology
Drug

uptake studies
junction investigation
Inflammation studies
Cell-cell

Features
Von

Willebrand factor (vWF) positive


positive
Smooth muscle -actin negative

Tunica media
(Smooth muscle cells)

CD31

The endothelium is the thin layer of endothelial cells that line the interior surface
of blood and lymphatic vessels - from the
aorta to the smallest capillaries. In large
vessels (arteries and veins), it is enclosed
by two other layers namely the tunica media and the tunica externa (see Fig. 1).
PromoCell endothelial cells (large vessels) are available from the umbilical
vein and artery, the aorta, the coronary
artery, the pulmonary artery, and the
saphenous vein. All endothelial cells are
tested for positive expression of vWF and
CD31, and the absence of smooth muscle
-actin.
Available endothelial cell types (large vessels):
HUVEC (see below)
HUAEC (see page 14)
HAoEC (see page 15)
HCAEC (see page 15)
HPAEC (see page 16)
HSaVEC (see page 16)

Human Umbilical Vein Endothelial


Cells (HUVEC)
HUVEC are isolated from the vein of the
umbilical cord and are commonly used
for physiological and pharmacological
investigations, such as macromolecule
transport, blood coagulation, angiogenesis, and fibrinolysis.
PromoCell offers HUVEC isolated
in standard Endothelial Cell Growth
Medium and also in Endothelial Cell

Tunica intima
(Endothelial cells)

Fig. 1: Cross section of an artery with the predominant cell types.

Fig. 2: HUVEC culture stained for vWF


(Factor VIII antigen). Nuclei counterstained with DAPI.

Growth Medium 2. The cells are supplied either from single donors* or from
pooled donors (from up to four different
umbilical cords).
In addition, HUVEC are available which
are pre-screened for VEGF (vascular
endothelial growth factor) response.
VEGF is an important signaling protein
involved in both vasculogenesis and
angiogenesis. In vitro, VEGF has been
shown to stimulate endothelial cell
mitogenesis, cell migration, sprouting, and microvascular permeability.

*Human Umbilical Artery Endothelial Cells (HUAEC) from the same donor are available on request.

Fig. 3: HUVEC culture stained for CD31


antigen. Nuclei counterstained with
DAPI.

Recommended media & reagents


Cell Growth Medium
(see page 63)
Endothelial Cell Growth Medium 2
(see page 63)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Endothelial

Human Primary
Cells

Tunica externa
(Connective tissue)

14

1 Human Primary Cells

Human Primary
Cells

Related products
HUVEC single donor Pellet
(see page 92)
HUVEC pooled Pellet (see page 92)
HUVEC Growth Medium 2 single
donor Pellet (see page 92)
HUVEC Growth Medium 2 pooled
Pellet (see page 92)
HUVEC pre-screened Pellet
(see page 92)

Human

Umbilical Artery Endothelial


Cells (see below)
PromoFectin-HUVEC Transfection
Reagent (see page 158)
VEGF (see page 179)
CD31 and VEGF Antibodies
(see page 169)
Fig. 4: HUVEC culture in phase contrast.

https://www.promocell.com/shop
Product

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12200
C-12250

1x106 cells in RNAlater

C-14010

500,000 cryopreserved cells


500,000 proliferating cells

C-12203
C-12253

1x106 cells in RNAlater

C-14011

Human Umbilical Vein Endothelial Cells


(HUVEC) isolated in Growth Medium 2,
single donor

500,000 cryopreserved cells


500,000 proliferating cells

C-12206
C-12207

HUVEC Growth Medium 2 single donor


Pellet

1x106 cells in RNAlater

C-14008

Human Umbilical Vein Endothelial Cells


(HUVEC) isolated in Growth Medium 2,
pooled

500,000 cryopreserved cells


500,000 proliferating cells

C-12208
C-12209

HUVEC Growth Medium 2 pooled Pellet

1x106 cells in RNAlater

C-14009

500,000 cryopreserved cells


500,000 proliferating cells

C-12205
C-12255

1x106 cells in RNAlater

C-14012

Human Umbilical Vein Endothelial Cells


(HUVEC) single donor
HUVEC single donor Pellet
Human Umbilical Vein Endothelial Cells
(HUVEC) pooled
HUVEC pooled Pellet

Human Umbilical Vein Endothelial Cells


(HUVEC) pre-screened
HUVEC pre-screened Pellet

Human Umbilical Artery


Endothelial Cells (HUAEC)
HUAEC are isolated from the arteries of
the umbilical cord and are often used for
in vitro studies on atherosclerosis but
also for endothelial cell-dependent lymphocyte activation and chemoattractant
activity experiments*.

Recommended media & reagents


Endothelial Cell Growth Medium
(see page 63)
Endothelial Cell Growth Medium 2
(see page 63)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Related products
Pellet (see page 92)
Human Umbilical Vein Endothelial
Cells (see page 13)
Human Umbilical Artery Smooth
Muscle Cells (see page 39)
PromoFectin-HUVEC Transfection
Reagent (see page 158)
CD31 Antibody
(see page 169)
HUAEC

https://www.promocell.com/shop
Product
Human Umbilical Artery Endothelial Cells
(HUAEC)
HUAEC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12202
C-12252

1x106 cells in RNAlater

C-14013

*Human Umbilical Vein Endothelial Cells (HUVEC) from the same donor are available on request.

1 Human Primary Cells

HAoEC are isolated from the human


ascending (thoracic) and descending (abdominal) aorta. They are useful
for studying vascular diseases such as
thrombosis, atherosclerosis, and hypertension as well as for stent-graft compatibility testing.

Recommended media & reagents


Cell Growth Medium MV
(see page 64)
Endothelial Cell Growth Medium MV2
(see page 64)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Endothelial

Related products
HAoEC Pellet (see page 91)
Human Aortic Smooth Muscle Cells
(see page 37)
Human Aortic Adventitial Fibroblasts
(see page 28)
PromoFectin-HUVEC Transfection
Reagent (see page 158)
CD31 Antibody
(see page 169)
https://www.promocell.com/shop

Product
Human Aortic Endothelial Cells (HAoEC)
HAoEC Pellet

Human Coronary Artery


Endothelial Cells (HCAEC)
HCAEC are isolated from the coronary
arteries (the right and the left coronary
artery, including the anterior descending and the circumflex branches) from a

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12271
C-12272

1x106 cells in RNAlater

C-14023

single donor. They are crucially involved


in the regulation of coronary blood flow
and cardiac functions and are consequently useful for in vitro studies of
diseases such as thrombosis, atherosclerosis, and hypertension. HCAEC are also
suitable for studying functional vaso-

dilators, such as prostacyclin, bradykinin,


or nitric oxide.
Recommended media & reagents
Cell Growth Medium MV
(see page 64)
Endothelial Cell Growth Medium MV2
(see page 64)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Endothelial

Related products
Pellet (see page 91)
Human Coronary Artery Smooth
Muscle Cells (see page 38)
PromoFectin-HUVEC Transfection
Reagent (see page 158)
CD31 Antibody
(see page 169)
HCAEC

Left main coronary


artery

Circumflex coronary
artery

Right coronary artery


Left anterior descending
coronary artery
Fig. 5: Location of the coronary arteries.

https://www.promocell.com/shop
Product
Human Coronary Artery Endothelial Cells
(HCAEC)
HCAEC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12221
C-12222

1x106 cells in RNAlater

C-14022

Human Primary
Cells

Human Aortic Endothelial Cells


(HAoEC)

15

16

1 Human Primary Cells

Human Primary
Cells

Human Pulmonary Artery


Endothelial Cells (HPAEC)
HPAEC are isolated from human pulmonary arteries (the main pulmonary
artery, left and right branches). Since the
pulmonary arteries carry blood from the
heart to the lungs, they are the only arteries (other than umbilical arteries) that
carry deoxygenated blood.

Recommended media & reagents


Cell Growth Medium
(see page 63)
Endothelial Cell Growth Medium 2
(see page 63)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Endothelial

Related products
Pellet (see page 92)
Human Pulmonary Artery Smooth
Muscle Cells (see page 38)
PromoFectin-HUVEC Transfection
Reagent (see page 158)
CD31 Antibody
(see page 169)
HPAEC

https://www.promocell.com/shop
Product
Human Pulmonary Artery Endothelial Cells
(HPAEC)
HPAEC Pellet

Human Saphenous Vein


Endothelial Cells (HSaVEC)
HSaVEC are isolated from human saphenous veins from single donors. The
saphenous vein is often used for autotransplantation in coronary artery bypass
operations, when arterial grafts are not
available or many grafts are required.

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12241
C-12242

1x106 cells in RNAlater

C-14024

Recommended media & reagents


Cell Growth Medium
(see page 63)
Endothelial Cell Growth Medium 2
(see page 63)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Endothelial

Related products
Pellet (see page 92)
PromoFectin-HUVEC Transfection
Reagent (see page 158)
CD31 Antibody
(see page 169)
HSaVEC

https://www.promocell.com/shop
Product
Human Saphenous Vein Endothelial Cells
(HSaVEC)
HSaVEC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12231
C-12232

1x106 cells in RNAlater

C-14025

1 Human Primary Cells

17

1.4 Endothelial Cells (Microvascular)


Applications
Angiogenesis

research

Oncology
Drug

uptake studies
junction investigation
Inflammation studies

at the interface between the circulating


fluid in the lumen and the surrounding
tissue, microvascular endothelial cells are
highly active and closely involved in numerous physiological processes.

Features
Von

Willebrand factor (vWF) positive


positive
Smooth muscle -actin negative
Cryopreserved at second passage
CD31

The walls of capillaries are composed of a


single layer of microvascular endothelial
cells. This layer is so thin that molecules
such as oxygen, water, and lipids can pass
through it by diffusion and enter the surrounding tissues. Waste products such
as carbon dioxide and urea, on the other
hand, diffuse back into the blood to be
carried away. Because of their location

Human Dermal Microvascular


Endothelial Cells (HDMEC)
HDMEC are isolated from the dermis of
juvenile foreskin and adult skin (different locations). Since the dermis contains
blood and lymphatic capillaries, HDMEC
comprise Blood and Lymphatic Microvascular Endothelial Cells. Both have a
common origin and can be identified by
several markers.
In addition, HDMEC are available which
are pre-screened for VEGF (vascular endothelial growth factor) response. VEGF
is an important signaling protein involved
in both vasculogenesis and angiogenesis.
In vitro, VEGF has been shown to stimu-

Microvascular endothelial cells differ in


morphologies and properties according
to the tissues supplied by the capillaries.
Therefore, PromoCell microvascular endothelial cells are available from dermal,
lung, cardiac, uterine, bladder, and brain
tissues.
Available endothelial cell types (microvascular):
HDMEC (see below)
HDBEC (see page 18)
HDLEC (see page 19)
HBdMEC (see page 20)
HCMEC (see page 20)
HPMEC (see page 21)
HUtMEC (see page 21)
HBMEC (see page 22)

late endothelial cell mitogenesis, cell


migration, sprouting, and microvascular
permeability.

Fig. 1: HDMEC culture stained for vWF


(Factor VIII antigen). Nuclei counterstained with DAPI.

Fig. 2: HDMEC culture stained for CD31


antigen. Nuclei counterstained with DAPI.

Endothelial

Pericytes (see page 51)


Transfection
Reagent (see page 158)
VEGF (see page 179)
CD31 Antibody
(see page 169)

Related products
Pellet (see page 91)
HDMEC adult Pellet (see page 91)
HDMEC pre-screened Pellet
(see page 91)

Fig. 3: HDMEC culture in phase contrast.

Recommended media & reagents


Cell Growth Medium MV
(see page 64)
Endothelial Cell Growth Medium MV2
(see page 64)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Human

PromoFectin-HUVEC

HDMEC

https://www.promocell.com/shop
Product
Human Dermal Microvascular Endothelial
Cells (HDMEC) juvenile foreskin
HDMEC Pellet
Human Dermal Microvascular Endothelial
Cells (HDMEC) adult donor
HDMEC adult Pellet
Human Dermal Microvascular Endothelial
Cells (HDMEC) pre-screened
HDMEC pre-screened Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12210
C-12260

1x106 cells in RNAlater

C-14015

500,000 cryopreserved cells


500,000 proliferating cells

C-12212
C-12262

1x106 cells in RNAlater

C-14016

500,000 cryopreserved cells


500,000 proliferating cells

C-12215
C-12265

1x106 cells in RNAlater

C-14017

Human Primary
Cells

Cell-cell

18

1 Human Primary Cells

HDBEC are isolated from the dermis of


juvenile foreskin and adult skin (different
locations) from a single donor*.
The cells are routinely analyzed by flow
cytometric analysis and immunofluorescent staining: they are > 95% CD31
positive (see Fig. 4) and stain positive for
von Willebrand factor. In addition, they
are > 90% negative for podoplanin
(see Fig. 5) and stain negative for
smooth muscle specific -actin.

Blood Endothelial Cells have a key function in physiological processes like vessel
tonus, capillary permeability, blood coagulation, fibrolysis, and angiogenesis.
They are also useful for disease studies
of atherosclerosis, tumorigenesis, and
thrombosis.
Recommended media & reagents
Cell Growth Medium MV
(see page 64)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Related products
Pellet (see page 91)
HDBEC adult Pellet (see page 91)
Human Dermal Lymphatic Endothelial
Cells (see page 19)
PromoFectin-HUVEC Transfection
Reagent (see page 158)
Podoplanin, CD31, and Prox-1
Antibodies (see page 169)
HDBEC

Endothelial

Count
75

CD31 negative

Cell Lab Quanta SC TM, Beckman Coulter, Inc.

CD31 positive

50

25

Fig. 4: Flow cytometric analysis of PromoCell HDBEC.


98% of the cells are CD31 positive .

0
0

10

102

103

104

CD31

Count
75

Podoplanin negative

Podoplanin positive
Cell Lab Quanta SC TM, Beckman Coulter, Inc.

Human Primary
Cells

Human Dermal Blood Endothelial


Cells (HDBEC)

50

25

Fig. 5: Flow cytometric analysis of PromoCell HDBEC.


91% of the cells are podoplanin negative.

0
0

10

102

103

104

Podoplanin

https://www.promocell.com/shop
Product
Human Dermal Blood Endothelial Cells
(HDBEC) juvenile foreskin
HDBEC Pellet
Human Dermal Blood Endothelial Cells
(HDBEC) adult donor
HDBEC adult Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12211
C-12214

1x106 cells in RNAlater

C-14018

500,000 cryopreserved cells


500,000 proliferating cells

C-12225
C-12226

1x106 cells in RNAlater

C-14019

*Human Dermal Lymphatic Microvascular Endothelial Cells (HDLEC) from the same donor are available on request.

1 Human Primary Cells

HDLEC are isolated from the dermis of


juvenile foreskin and adult skin (different
locations) from a single donor*. The cells
are routinely analyzed by flow cytometric
analysis and immunofluorescent staining:
> 95% of the cells are CD31 positive and
podoplanin positive (see Fig. 6 and 7).
In addition, the cells stain positive for
Prox-1.
Lymphatic vessels transport excess fluids
from tissues to the circulatory system and
are a major component of the immune

system. The transported fluid is nearly


cell free. The vessels are highly permeable, because they lack a continuous
basal membrane. Lymphatic endothelial
cells are involved in pathological alterations of the lymphatic system. During
tumor lymphangiogenesis, the lymphatic
endothelial cells build new vessels that
infiltrate tumors, attract tumor cells, and
induce tumor cell metastasis.
Recommended media & reagents
Cell Growth Medium MV2
(see page 64)
Endothelial

DetachKit
Cryo-SFM

(see page 95)


(see page 79)

Related products
Pellet (see page 91)
HDLEC adult Pellet (see page 91)
Human Dermal Blood Endothelial
Cells (see page 18)
PromoFectin-HUVEC Transfection
Reagent (see page 158)
VEGF (see page 179)
Podoplanin, CD31, and Prox-1
Antibodies (see page 169)
HDLEC

Count
75

CD31 positive
Cell Lab Quanta SC TM, Beckman Coulter, Inc.

CD31 negative

50

25

Fig. 6: Flow cytometric analysis of PromoCell HDLEC.


99% of the cells are CD31 positive.

0
0

10

102

103

104

CD31

Count
75

Podoplanin positive
Cell Lab Quanta SC T M, Beckman Coulter, Inc.

Podoplanin negative

50

25

Fig. 7: Flow cytometric analysis of PromoCell HDLEC.


96% of the cells are podoplanin positive.

0
0

10

102

103

104

Podoplanin

https://www.promocell.com/shop
Product
Human Dermal Lymphatic Endothelial
Cells (HDLEC) juvenile foreskin
HDLEC Pellet
Human Dermal Lymphatic Endothelial
Cells (HDLEC) adult donor
HDLEC adult Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12216
C-12218

1x106 cells in RNAlater

C-14020

500,000 cryopreserved cells


500,000 proliferating cells

C-12217
C-12219

1x106 cells in RNAlater

C-14021

*Human Dermal Blood Endothelial Cells (HDBEC) from the same donor are available on request.

Human Primary
Cells

Human Dermal Lymphatic


Endothelial Cells (HDLEC)

19

20

1 Human Primary Cells

Human Primary
Cells

Human Bladder Microvascular


Endothelial Cells (HBdMEC)
HBdMEC are isolated from the human
bladder microvasculature. The cells are
routinely analyzed by immunofluorescent staining: they stain positive for
CD31 and von Willebrand factor and
negative for smooth muscle -actin.
HBdMEC are useful in studying the
role of microvascular endothelial
cells in the control of inflammation,
capillary exchange, tissue growth, and
angiogenesis.

Recommended media & reagents


Cell Growth Medium MV
(see page 64)
Endothelial Cell Growth Medium MV2
(see page 64)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Endothelial

Related products
Pellet (see page 91)
PromoFectin-HUVEC Transfection
Reagent (see page 158)
CD31 Antibody
(see page 169)
HBdMEC

https://www.promocell.com/shop
Product
Human Bladder Microvascular Endothelial
Cells (HBdMEC)
HBdMEC Pellet

Human Cardiac Microvascular


Endothelial Cells (HCMEC)
HCMEC are isolated from heart ventricles from single donors. The cells are
routinely analyzed by immunofluorescent staining: they stain positive for
CD31 and von Willebrand factor and
negative for smooth muscle -actin.
HCMEC intensively interact with cardiomyocytes and therefore have a characteristic phenotype different from other
microvascular endothelial cells. HCMEC

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12291
C-12292

1x106 cells in RNAlater

C-14026

play an important role in the physiological regulation of coronary blood flow


and capillary exchange. Medical conditions such as hypertrophy, ischemia,
hypertension, and diabetes mellitus are
associated with endothelial dysfunction.
Recommended media & reagents
Cell Growth Medium MV
(see page 64)
Endothelial Cell Growth Medium MV2
(see page 64)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Endothelial

Related products
HCMEC Pellet (see page 91)
Human Cardiac Myocytes (see page 11)
Human Cardiac Fibroblasts
(see page 28)
PromoFectin-HUVEC Transfection
Reagent (see page 158)
CD31 Antibody
(see page 169)

https://www.promocell.com/shop
Product
Human Cardiac Microvascular
Endothelial Cells (HCMEC)
HCMEC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12285
C-12286

1x106 cells in RNAlater

C-14029

1 Human Primary Cells

HPMEC are isolated from the lung from


a single donor. The cells are routinely
analyzed by immunofluorescent staining: they stain positive for CD31 and
von Willebrand factor and negative for
smooth muscle -actin.
The lung has a vast endothelial surface
area, which is essential for the exchange
of gases. HPMEC are most appropriate
for studying human lung diseases.

Recommended media & reagents


Cell Growth Medium MV
(see page 64)
Endothelial Cell Growth Medium MV2
(see page 64)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Endothelial

Related products
Pellet (see page 92)
Human Pulmonary Fibroblasts
(see page 28)
Human Small Airway Epithelial Cells
(see page 24)
PromoFectin-HUVEC Transfection
Reagent (see page 158)
CD31 Antibody
(see page 169)
HPMEC

https://www.promocell.com/shop
Product
Human Pulmonary Microvascular
Endothelial Cells (HPMEC)
HPMEC Pellet

Human Uterine Microvascular


Endothelial Cells (HUtMEC)
HUtMEC are isolated from the myometrium (middle layer of the uterine wall)
from a single donor, who has not been
pre-treated with hormones. The cells
are routinely analyzed by immunofluorescent staining: they stain positive for
CD31 and von Willebrand factor and
negative for smooth muscle -actin.
During pregnancy HUtMEC propagate
intensely and change their phenotype
and expression pattern in response to
sex steroids. This includes up-regulation

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12281
C-12282

1x106 cells in RNAlater

C-14027

of HUtMEC nitric oxide synthase activity, modulation of adhesion molecule


expression, and an increase in endothelial cell survival and angiogenic activity.
HUtMEC are involved in medical conditions such as uterine fibroma or adenomyosis.
Recommended media & reagents
Cell Growth Medium MV
(see page 64)
Endothelial Cell Growth Medium MV2
(see page 64)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Endothelial

Related products
Pellet (see page 92)
Human Uterine Fibroblasts
(see page 29)
Human Uterine Smooth Muscle Cells
(see page 41)
PromoFectin-HUVEC Transfection
Reagent (see page 158)
CD31 Antibody
(see page 169)
HUtMEC

https://www.promocell.com/shop
Product
Human Uterine Microvascular
Endothelial Cells (HUtMEC)
HUtMEC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12295
C-12296

1x106 cells in RNAlater

C-14028

Human Primary
Cells

Human Pulmonary Microvascular


Endothelial Cells (HPMEC)

21

22

1 Human Primary Cells

Human Primary
Cells

Human Brain Microvascular


Endothelial Cells (HBMEC)
Human Brain Microvascular Endothelial
Cells (HBMEC) are isolated from cortical
tissue (see Fig. 8) from a single donor.
They are routinely analyzed by flow
cytometry and immunofluorescent staining. The cells are positive for CD31 and
von Willebrand factor and negative for
smooth muscle -actin and CD90.

Brain microvascular endothelial cells are


highly specialized cells responsible for
the formation and functional properties
of the Blood-Brain Barrier (BBB). The
BBB is characterized by cellular tight
junctions and active transport systems
which allow the selective transport of
substances into the brain, and prevent
the entrance of unwanted substances.
Therefore, PromoCell HBMEC are well
suited for studying brain endothelial cell

functions like active transport systems


and drug uptake. Furthermore, neuroinflammation studies as well as tight
junction research can be performed
with HBMEC.
Recommended media & reagents
Cell Growth Medium MV2
(see page 64)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Endothelial

Cerebral cortex
Thalamus
Corpus callosum

Hypothalamus
Pituitary gland
Cerebellum
Spinal cord

Fig. 8: Schematic structure of the brain.

Fig. 9: HBMEC in phase contrast.

https://www.promocell.com/shop
Product
Human Brain Microvascular Endothelial
Cells (HBMEC)
HBMEC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

Limited Availability

1x106 cells in RNAlater

Limited Availability

Limited Availability

C-12287
C-12288
C-14032

1 Human Primary Cells

23

1.5 Epithelial Cells

Drug uptake studies


Toxicological research
Wound healing research
Oncology
Cell differentiation studies
Respiratory disease research

Typically, epithelial tissue is made of


tightly packed cells with little intercellular space and is arranged in flat sheets
forming effective barriers between the
underlying tissues and the external environment.

Cytokeratin positive
Available from different tissues
Cryopreserved at second passage

PromoCell offers epithelial cells from different locations:


Airways: Nasal mucosa, trachea, bronchi,
and distal respiratory tract
Breast: Mammary gland
Kidney: Whole kidney and renal cortex
Placenta: Amniotic membrane

The epithelium is the interface between the


body and the external environment. It covers all outside surfaces and inside lumina
e.g. the inner lining of the respiratory
tract. In addition, epithelial cells also make
up exocrine and endocrine glands. The
functions of epithelial cells are diverse and
include absorption, secretion, protection,
transcellular transport, and sensation.

Available Epithelial Cells:


HNEpC (see below)
HTEpC (see below)
HBEpC (see page 24)
HSAEpC (see page 24)
HMEpC (see page 25)
HREpC (see page 25)
HRCEpC (see page 26)
HPlEpC (see page 26)

Human Nasal Epithelial Cells


(HNEpC)

particles that are subsequently transported to the pharynx by cilia on the


epithelial cells. HNEpC are useful for in
vitro studies of these processes.

Features

HNEpC are isolated from normal human


nasal mucosa and stain positive for cytokeratin.
The epithelium in the nasal cavity cleans,
humidifies, and warms inhaled air. In addition, it produces mucus, which binds

Fig. 1: HTEpC culture in phase contrast.


Note the cuboidal shape typical for airway epithelial cells.

Fig. 2: HTEpC culture stained for cytokeratin. Nuclei counterstained with


DAPI.

DetachKit
Cryo-SFM

(see page 95)


(see page 79)

Related products
Pellet (see page 92)
PromoFectin Transfection Reagent
(see page 157)
HNEpC

Recommended media & reagents


Epithelial Cell Growth Medium
(see page 65)

Airway

https://www.promocell.com/shop
Product
Human Nasal Epithelial Cells (HNEpC)
HNEpC Pellet

Human Tracheal Epithelial Cells


(HTEpC)
HTEpC are isolated from the surface
epithelium of human trachea and stain
positive for cytokeratin.
The respiratory epithelia are responsible for the lubrication of the lungs,
the maintenance of humidity, and the
cleaning of the respiratory tract. They

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12620
C-12621

1x106 cells in RNAlater

C-14062

are an important target for drugs, toxins, and carcinogens. In addition, they
are involved in many diseases such as
cystic fibrosis. Consequently, HTEpC are
useful for investigating the function and
pathology of the respiratory system.
Recommended media & reagents
Epithelial Cell Growth Medium
(see page 65)

Airway

DetachKit
Cryo-SFM

(see page 95)


(see page 79)

Related products
Pellet (see page 92)
Human Tracheal Smooth Muscle Cells
(see page 39)
PromoFectin Transfection Reagent
(see page 157)
HTEpC

Human Primary
Cells

Applications

24

1 Human Primary Cells

https://www.promocell.com/shop
Product
Human Tracheal Epithelial Cells (HTEpC)

Human Primary
Cells

HTEpC Pellet

Human Bronchial Epithelial Cells


(HBEpC)
HBEpC are isolated from the surface
epithelium of human bronchi and stain
positive for cytokeratin.
The respiratory epithelia are responsible for the lubrication of the lungs,
the maintenance of humidity, and the
cleaning of the respiratory tract. They

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12644
C-12645

1x106 cells in RNAlater

C-14064

are an important target for drugs, toxins, and carcinogens. In addition, they
are involved in many diseases such as
cystic fibrosis. Consequently, HBEpC are
useful for investigating the function and
pathology of the respiratory system.
Recommended media & reagents
Epithelial Cell Growth Medium
(see page 65)

Airway

DetachKit
Cryo-SFM

(see page 95)


(see page 79)

Related products
Pellet (see page 91)
Human Bronchial Smooth Muscle
Cells (see page 40)
PromoFectin Transfection Reagent
(see page 157)
HBEpC

https://www.promocell.com/shop
Product
Human Bronchial Epithelial Cells (HBEpC)
HBEpC Pellet

Human Small Airway Epithelial


Cells (HSAEpC)
HSAEpC are isolated from the distal portion of the human respiratory tract in the
1 mm bronchiole area. They stain positive for cytokeratin.
The distal respiratory tract mainly consists of pulmonary alveoli, which are
spherical outcroppings of the respiratory
bronchioles and are the primary sites of
gas exchange with the blood.
HSAEpC are qualified for functional

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12640
C-12641

1x106 cells in RNAlater

C-14063

studies to investigate disorders such as


asthma or pulmonary inflammation.

Recommended media & reagents


Airway Epithelial Cell
Growth Medium (see page 66)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Small

Related products
Pellet (see page 92)
Human Pulmonary Microvascular
Endothelial Cells (see page 21)
Human Pulmonary Fibroblasts
(see page 28)
PromoFectin Transfection Reagent
(see page 157)
HSAEpC

Fig. 3: HSAEpC culture in phase contrast.

https://www.promocell.com/shop
Product
Human Small Airway Epithelial Cells
(HSAEpC)
HSAEpC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12642
C-12643

1x106 cells in RNAlater

C-14065

1 Human Primary Cells

HMEpC are isolated from the human


adult mammary gland and stain positive
for cytokeratin.
Hormones extensively control the development of the mammary glands.
Throughout puberty, the glands begin to
develop in females in response to ovarian
hormones. Further on during pregnancy,
rising levels of estrogen and progesterone cause additional growth and differentiation. Finally, due to the presence of
the hormone prolactin in late pregnancy,

milk secretion (lactation) begins.


Since mammary gland cells can easily be
induced to grow and multiply by hormones, HMEpC are very well suited for
developmental investigations. In addition, they are useful for breast cancer in
vitro research since breast cancer usually
originates in the lobules or ducts of the
mammary glands.
Recommended media & reagents
Epithelial Cell Growth
Medium (see page 67)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Related products
Pellet (see page 91)
PromoFectin Transfection Reagent
(see page 157)
HMEpC

Human Primary
Cells

Human Mammary Epithelial Cells


(HMEpC)

25

Mammary

Fig. 4: HMEpC culture in phase contrast.

https://www.promocell.com/shop
Product
Human Mammary Epithelial Cells
(HMEpC)
HMEpC Pellet

Human Renal Epithelial Cells


(HREpC)
HREpC are isolated from the adult kidney and stain positive for cytokeratin.
Kidneys have a very complex structure
and contain diverse epithelia such as
those in the convoluted tubules, the
cortical collecting ducts, the calyxes,

Renal cortex

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12650
C-12651

1x106 cells in RNAlater

C-14066

and the renal pelvis. The epithelial cells


present in the distinct ducts and tubules
differ in their morphology and physiological function. Consequently, HREpC
are comprised of a heterogeneous population of epithelial cells.
Important physiological processes like
osmoregulation and excretion can be
studied with HREpC in vitro.

Recommended media & reagents


Epithelial Cell Growth
Medium 2 (see page 68)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Renal

Related products
Pellet (see page 92)
Human Renal Cortical Epithelial Cells
(see page 26)
PromoFectin Transfection Reagent
(see page 157)
HREpC

Renale capsule
Renal medulla
Renal calices
Renal pelvis
Ureter

Fig. 5: Schematic structure of a kidney.

Fig. 6: HREpC culture in phase contrast.

26

1 Human Primary Cells

https://www.promocell.com/shop
Product
Human Renal Epithelial Cells (HREpC)

Human Primary
Cells

HREpC Pellet

Human Renal Cortical Epithelial


Cells (HRCEpC)
HRCEpC are isolated from the cortex of
the human kidney and stain positive for
cytokeratin. The renal cortex is the outer
portion of the kidney between the renal
capsule and the renal medulla. It contains the renal corpuscles, the proximal
and distal convoluted tubules, and the

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12665
C-12666

1x106 cells in RNAlater

C-14067

cortical collecting ducts.


Important physiological processes like
osmoregulation and excretion can be
studied with HRCEpC in vitro.
Recommended media & reagents
Renal Epithelial Cell Growth
Medium 2 (see page 68)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Related products
Pellet (see page 92)
Human Renal Epithelial Cells
(see page 25)
PromoFectin Transfection Reagent
(see page 157)
HRCEpC

https://www.promocell.com/shop
Product
Human Renal Cortical Epithelial Cells
(HRCEpC)
HRCEpC Pellet

Human Placental Epithelial Cells


(HPlEpC)
HPlEpC are isolated from the amniotic
membrane and stain positive for cytokeratin.
Placental Epithelial Cells are of fetal origin and form a single layer at the inner
surface of the amnion. They protect the
fetus against maternal leukocytes, neo-

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12660
C-12662

1x106 cells in RNAlater

C-14068

plastic cells, and pathogens. Cultured


HPlEpC are suitable for physiological and
pathological studies of amniotic functions.
Recommended media & reagents
Epithelial Cell Growth
Medium (see page 69)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Related products
Villous Mesenchymal
Fibroblasts (see page 29)
HPlEpC Pellet (see page 92)
PromoFectin Transfection Reagent
(see page 157)
Human

Placental

https://www.promocell.com/shop
Product
Human Placental Epithelial Cells (HPlEpC)
HPIEpC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12395
C-12397

1x106 cells in RNAlater

C-14069

1 Human Primary Cells

27

1.6 Fibroblasts

Wound

healing research
engineering research
Regeneration studies
Toxicological research
Oncology
Epithelial-mesenchymal interaction
studies
Tissue

Features
CD90

positive

Fibroblasts are morphologically heterogeneous with diverse appearances


depending on their location in the body
and their activity. Injury of tissue displays
a proliferative stimulus for fibroblasts and
induces them to produce wound healing
proteins. Therefore, fibroblasts are well
suited for wound healing studies.

Fig. 1: NHDF culture in phase contrast.

Cryopreserved
Available

at second passage
from different tissues

like collagens, glycosaminoglycans,


and glycoproteins. The composition of
the extracellular matrix determines the
physical properties of connective tissues.

Human Primary
Cells

Applications

Fibroblasts are the most common cells


in connective tissue. Their main function
is to maintain the structural integrity of
the connective tissue by continuously
secreting extracellular matrix proteins

Normal Human Dermal Fibroblasts


(NHDF)
NHDF are isolated from the dermis of
juvenile foreskin or adult skin from different locations like the face, the breasts,
the abdomen, and the thighs*. They can
be used for wound healing studies and
dermatological research to investigate
diseases like scleroderma, fibrosarcoma,
fibrosis, xeroderma pigmentosum, and
histiocytoma. Moreover, fibroblasts are
important for cancer research, tissue
regeneration, and tissue engineering
studies.

Available Fibroblasts:
NHDF (see below)
HPF (see page 28)
HAoAF (see page 28)
HCF (see page 28)
HUF (see page 29)
HVMF (see page 29)

Fig. 2: NHDF culture stained for CD90.


Nuclei counterstained with DAPI.

Recommended media & reagents for


NHDF juvenile foreskin
Fibroblast Growth Medium
(see page 70)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Recommended media & reagents for
NHDF adult donor
Fibroblast Growth Medium 2
(see page 70)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Related products
Pellet (see page 92)
NHDF adult Pellet (see page 92)
Normal Human Epidermal
Keratinocytes (see page 32)
Normal Human Epidermal
Melanocytes (see page 33)
PromoFectin Transfection Reagent
(see page 157)
FGF-1 and FGF-2 (see page 179)
NHDF

https://www.promocell.com/shop
Product
Normal Human Dermal Fibroblasts (NHDF)
juvenile foreskin
NHDF Pellet
Normal Human Dermal Fibroblasts (NHDF)
adult donor
NHDF adult Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12300
C-12350

1x106 cells in RNAlater

C-14030

500,000 cryopreserved cells


500,000 proliferating cells

C-12302
C-12352

1x106 cells in RNAlater

C-14031

* Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are
available on request.

Human Primary
Cells

28

1 Human Primary Cells

Human Pulmonary Fibroblasts


(HPF)

pulmonary hypertension can also be investigated with cultured HPF.

HPF are isolated from human lung tissue. They are often used for studying
lung tissue repair, tissue remodeling after
injury, or the response after pulmonary
inflammation. Fibroblast disorders like
pulmonary fibrosis or hypoxia-induced

Recommended media & reagents


Fibroblast Growth Medium 2
(see page 70)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Related products
Pellet (see page 92)
Human Pulmonary Microvascular
Endothelial Cells (see page 21)
Human Small Airway Epithelial Cells
(see page 24)
PromoFectin Transfection Reagent
(see page 157)
FGF-1 and FGF-2 (see page 179)
HPF

https://www.promocell.com/shop
Product
Human Pulmonary Fibroblasts (HPF)
HPF Pellet

Human Aortic Adventitial


Fibroblasts (HAoAF)
HAoAF are isolated from human aortic
adventitial tissue. HAoAF can be used
for studies concerning vascular diseases
e.g. neointimal formation, vascular remodeling, atherosclerosis, and hypertension. They are also often used for the
examination of fibroblast disorders like

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12360
C-12361

1x106 cells in RNAlater

C-14035

fibrosis or other diseases linked to either


imperfect or excessive accumulation of
fibroblasts.
Recommended media & reagents
Fibroblast Growth Medium 2
(see page 70)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Related products
Pellet (see page 91)
Human Aortic Endothelial Cells
(see page 15)
Human Aortic Smooth Muscle Cells
(see page 37)
PromoFectin Transfection Reagent
(see page 157)
FGF-1 and FGF-2 (see page 179)
HAoAF

https://www.promocell.com/shop
Product
Human Aortic Adventitial Fibroblasts
(HAoAF)
HAoAF Pellet

Human Cardiac Fibroblasts (HCF)


HCF are isolated from the ventricles of
an adult heart. They play a central role in
the maintenance of the extracellular matrix in the normal heart and the synthesis
of growth factors and cytokines. Under
pathophysiological conditions, cardiac
fibroblasts are involved in scar formation

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12380
C-12381

1x106 cells in RNAlater

C-14037

after myocardial infarction, cardiac fibrosis, and cardiac hypertrophy. HCF can be
used to study such processes in vitro.
Recommended media & reagents
Fibroblast Growth Medium 3
(see page 71)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Related products
Pellet (see page 91)
Human Cardiac Myocytes (see page 11)
Human Cardiac Microvascular
Endothelial Cells (see page 20)
PromoFectin Transfection Reagent
(see page 157)
FGF-1 and FGF-2 (see page 179)
HCF

https://www.promocell.com/shop
Product
Human Cardiac Fibroblasts (HCF)
HCF Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12375
C-12377

1x106 cells in RNAlater

C-14036

1 Human Primary Cells

HUF are isolated from the uterine wall.


During pregnancy, the uterine wall undergoes deep but reversible structural
and biochemical changes. The uterine
wall is the target of hormones and
growth factors that act simultaneously
and lead to extensive modifications of
the extracellular matrix. This and other
morphological and biochemical effects
can be studied in vitro using HUF.

Recommended media & reagents


Fibroblast Growth Medium 2
(see page 70)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Related products
Pellet (see page 92)
Human Uterine Microvascular
Endothelial Cell (see page 21)
Human Uterine Smooth Muscle Cells
(see page 41)
PromoFectin Transfection Reagent
(see page 157)
FGF-1 and FGF-2 (see page 179)
HUF

https://www.promocell.com/shop
Product
Human Uterine Fibroblasts (HUF)
HUF Pellet

Human Villous Mesenchymal


Fibroblasts (HVMF)
HVMF are isolated from human villous
placental tissue. They can be used as
model systems in organ development
research. In addition, they are useful for
investigating the mechanism regulating
human placental growth and function.

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12385
C-12386

1x106 cells in RNAlater

C-14038

Recommended media & reagents


Fibroblast Growth Medium 2
(see page 70)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Related products
Pellet (see page 92)
Human Placental Epithelial Cells
(see page 26)
PromoFectin Transfection Reagent
(see page 157)
FGF-1 and FGF-2 (see page 179)
HVMF

https://www.promocell.com/shop
Product
Human Villous Mesenchymal Fibroblasts
(HVMF)
HVMF Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12390
C-12391

1x106 cells in RNAlater

C-14034

Human Primary
Cells

Human Uterine Fibroblasts (HUF)

29

30

1 Human Primary Cells

1.7 Follicle Dermal Papilla Cells


Applications
Hair

growth research
testing
Toxicological testing
Drug

Features

Recommended media & reagents


Follicle Dermal Papilla Cell Growth
Medium (see page 72)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Related products
Pellet (see page 91)
PromoFectin Transfection Reagent
(see page 157)
HFDPC

Human Primary
Cells

Alkaline

phosphatase positive
at second passage
Available from adult lateral scalp
Available from donors with different
hair colors
Cryopreserved

Human Follicle Dermal Papilla Cells


(HFDPC) are isolated from human dermis originating from the lateral scalp.
Information on donor hair and skin color
is available for each lot. HFDPC stain
positive for alkaline phosphatase.

Fig. 1: HFDPC culture in phase contrast.

Sebaceous gland
Hair shaft

Bulge

Matrix cells

Dermal papillae are embedded in a


laminin and collagen IV rich extracellular
matrix at the base of the hair follicles.
They are essential for the induction and
maintenance of hair growth. Since they
have androgen receptors, they can be
used for in vitro screening of androgen
blocking reagents.

Dermal papilla

Fig. 2: Diagram showing the location of the dermal papilla in the hair follicle.

https://www.promocell.com/shop
Product
Human Follicle Dermal Papilla Cells
(HFDPC)
HFDPC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12071
C-12072

1x106 cells in RNAlater

C-14005

1 Human Primary Cells

31

1.8 Hepatocytes

Drug

testing

Toxicological

testing
studies
Liver physiology research
Liver pathology research
Oncology
Metabolism

Features
Cryopreserved

immediately after
isolation
3 - 6 x 106 viable cells per cryovial
Adherence > 50%
Phase I reaction positive
Phase II reaction positive
Albumin positive
Human Hepatocytes (HHpC) are isolated from the normal livers of single
donors. After isolation, the cells are
immediately cryopreserved and then
intensively quality tested. Test criteria are cell viability, cell adherence
efficiency, phase I metabolism, phase II
metabolism, and albumin synthesis. The

cell adherence efficiency is at least 50%.


The liver is the main metabolic organ
in the body performing glycolysis,
glycogenesis, amino acid metabolism,
lipid metabolism, and ureagenesis. In
addition, it also regulates the blood
sugar level. Furthermore, hepatocytes
eliminate toxic substances soon after
absorption since the nutrition rich blood
from the gastro-intestinal system first
has to pass the liver. This detoxification consists of phase I and phase II
reactions. The most important phase I
enzyme system is the cytochrome P450
system. Consequently, cultured human
hepatocytes are perfectly suited for in
vitro metabolism and toxicity/detoxification studies prior to preclinical or
clinical tests.
Note: For culturing Hepatocytes,
Collagen type I coated culture vessels
are necessary.
Under standard in vitro cell culture
conditions, mature human hepatocytes
usually do not survive for longer than
10-14 days and do not proliferate.

Recommended media & reagents


Hepatocyte Growth Medium

(see page 73)
Hepatocyte Maintenance Medium
(see page 73)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Related products
Pellet (see page 91)
PromoFectin-Hepatocyte Transfection
Reagent (see page 158)
HHpC

Fig 1. HHpC culture in phase contrast.

Count
Living cells

Dead cells
Cell Lab Quanta SC TM, Beckman Coulter, Inc.

37,5

25

12,5

Fig. 2: Flow cytometric analysis of PromoCell HHpC


using 7-AAD staining. 77 % viable hepatocytes after
thawing of the cryopreserved cells.

0
0

10

102

103

104

7-AAD

https://www.promocell.com/shop
Product
Human Hepatocytes (HHpC)
HHpC Pellet

Size

Catalog Number

3 - 6 x 106 cryopreserved cells

C-12850

1x106 cells in RNAlater

C-14085

Human Primary
Cells

Applications

32

1 Human Primary Cells

1.9 Keratinocytes
Applications
Wound

healing research
development and
differentiation studies
Dermatological research
Drug uptake studies
Pharmaceutical testing
Cosmetic and toxicological testing
Oncology

Human Primary
Cells

Epidermal

Features

body and minimizes moisture loss.


Keratinocytes are also able to produce
a variety of cytokines, growth factors,
interleukins, and complement factors.
Therefore keratinocytes are important
for wound healing, inflammation, and
immune response.
Recommended media & reagents
Keratinocyte Growth Medium 2
(see page 74)
DetachKit (see page 95)
Cryo-SFM (see page 79)

positive
from juvenile foreskin or
adult skin from different locations
Available from single and pooled
donors

Related products
NHEK.f single donor Pellet (see page 92)
NHEK.f pooled Pellet (see page 92)
NHEK adult, single donor Pellet

(see page 92)
NHEK adult, pooled Pellet
(see page 92)
Normal Human Dermal Fibroblasts
(see page 27)
Normal Human Epidermal
Melanocytes (see page 33)
PromoFectin Transfection Reagent
(see page 157)

Cytokeratin
Available

Normal Human Epidermal Keratinocytes (NHEK) are available from single or


pooled donors isolated from the epidermis
of juvenile foreskin or adult skin from different locations like the face, the breasts,
the abdomen, and the thighs*. They are
the major cell type in the epidermis, making up about 90% of the cells.
Epidermal keratinocytes originate in the
stratum basale and move up through
the layers of the epidermis. During this
movement, they undergo gradual differentiation and morphology changes until
they reach the stratum corneum, where
they form a layer of nucleus-free, flat,
and highly keratinized squamous cells.
This layer forms an effective barrier to
the entry of infectious agents into the

Stratum corneum
Stratum granulosum
Stratum spinosum

Stratum basale

Fig. 1: Structure of the epidermis.

Fig. 2: NHEK culture in phase contrast.


Note the cobblestone shape typical for
keratinocytes.

Fig. 3: NHEK culture stained for Cytokeratin. Nuclei counterstained with


DAPI.

https://www.promocell.com/shop
Product
Normal Human Epidermal Keratinocytes
(NHEK) juvenile foreskin, single donor
NHEK.f single donor Pellet
Normal Human Epidermal Keratinocytes
(NHEK) juvenile foreskin, pooled
NHEK.f pooled Pellet
Normal Human Epidermal Keratinocytes
(NHEK) adult, single donor
NHEK adult, single donor Pellet
Normal Human Epidermal Keratinocytes
(NHEK) adult, pooled
NHEK adult, pooled Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12001
C-12002

1x106 cells in RNAlater

C-14001

500,000 cryopreserved cells


500,000 proliferating cells

C-12005
C-12007

1x106 cells in RNAlater

C-14003

500,000 cryopreserved cells


500,000 proliferating cells

C-12003
C-12004

1x106 cells in RNAlater

C-14002

500,000 cryopreserved cells


500,000 proliferating cells

C-12006
C-12008

1x106 cells in RNAlater

C-14004

* Normal Human Dermal Fibroblasts (NHDF) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available
on request.

1 Human Primary Cells

33

1.10 Melanocytes

Dermatological

research
research
Melanogenesis studies
Cosmetic and toxicological testing
Skin compound screening
Melanoma

Features
Available

from juvenile foreskin or


adult skin from different locations
Available from light, moderate, and
dark skin types
Donor hair and eye color available on
request
Mel-5 positive
Cultured in PMA-containing or
PMA-free Medium
Normal Human Epidermal Melanocytes
(NHEM) are isolated from the epidermis
of juvenile foreskin or adult skin from
different locations including the face, the
breasts, the abdomen, and the thighs*.
They are located in the stratum basale,
but branch out between the keratinocytes in suprabasal layers (see Fig. 1).
About 5-10 % of the cells in the epidermis are melanocytes.
The main purpose of melanocytes is
the production of melanin, the protein
responsible for the pigmentation of the
skin, eyes, and hair. Melanin protects the
cells in the skin and in deeper layers from
the hazardous effects of UV radiation. It
is produced and stored in melanosomes,
which are located close to the melanocyte membrane, but it can also be transferred to neighboring keratinocytes.
PromoCell melanocytes are isolated

using either the serum-free, PMA (Phorbol Myristate Acetate)-containing Melanocyte Growth Medium or the serumfree and PMA-free Melanocyte Growth
Medium M2.
Since PMA is a tumor promoting mitogen, it can interfere with experimental
approaches. Therefore, we recommend using cells isolated in Melanocyte
Growth Medium M2 and also using this
medium for cultivation.
Recommended media & reagents
Melanocyte Growth Medium
(see page 75)

Melanocyte

Growth Medium M2
(see page 75)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Related products
Pellet (see page 92)
NHEM.f M2 Pellet (see page 92)
NHEM M2 adult Pellet (see page 92)
Normal Human Dermal Fibroblasts
(see page 27)
Normal Human Epidermal
Keratinocytes (see page 32)
PromoFectin Transfection Reagent
(see page 157)
NHEM.f

Stratum corneum
Stratum granulosum
Stratum spinosum

Stratum basale

Melanosoma
Keratinocytes
Melanocyte

Fig. 1: Structure of the epidermis with


the location of a melanocyte.

Fig. 2: NHEM cultured in Melanocyte


Growth Medium in phase contrast.

Fig. 3: NHEM cultured in Melanocyte


Growth Medium M2 in phase contrast.

https://www.promocell.com/shop
Product
Normal Human Epidermal Melanocytes
(NHEM) juvenile foreskin
NHEM.f Pellet
Normal Human Epidermal Melanocytes
(NHEM) juvenile foreskin, cultured in M2
Medium
NHEM.f M2 Pellet
Normal Human Epidermal Melanocytes
(NHEM) adult donor, cultured in M2
Medium
NHEM M2 adult Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12400
C-12450

1x106 cells in RNAlater

C-14040

500,000 cryopreserved cells


500,000 proliferating cells

C-12402
C-12452

1x106 cells in RNAlater

C-14042

500,000 cryopreserved cells


500,000 proliferating cells

C-12403
C-12453

1x106 cells in RNAlater

C-14043

* Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Dermal Fibroblasts (NHDF) from the same donor are available on request.

Human Primary
Cells

Applications

34

1 Human Primary Cells

1.11 Osteoblasts
Applications
Bone

differentiation
remodeling
Bone physiology research
Oncology
Inflammatory studies
Osteoporosis research
Human Primary
Cells

Bone

Features
From

knee and hip femoral tissue


at second passage
Alkaline phosphatase positive
Tested for mineralization
Growth and Mineralization Media
available
Cryopreserved

Human Osteoblasts (HOB) are isolated


from femoral trabecular bone tissue from
the knee or hip joint region (lot specific
source information is available on request). They stain positive for alkaline
phosphatase and can be mineralized using PromoCell Mineralization Medium.
Osteoblasts are of mesenchymal origin
and are mainly responsible for bone formation and remodeling. In vivo, they
produce a collagen type I rich extracellular
matrix called the osteoid.
During their development, osteoblasts
undergo a maturation process called
osteogenesis. When they become encased in the bone matrix, they stop proliferating and become osteocytes.

Recommended media & reagents


Osteoblast Growth Medium
(see page 76)
Osteoblast Mineralization Medium
(see page 76)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Fig. 2: Alkaline phosphatase activity in


HOB stained with BCIP/NBT.

Fig. 3: Mineralized HOB. Calcium deposits stained with Alizarin Red S.

Related products
Pellet (see page 92)
PromoFectin Transfection Reagent
(see page 157)
HOB

For more information please see


www.promocell.com/knowledge-base

Fig. 1: HOB culture in phase contrast.

https://www.promocell.com/shop
Product
Human Osteoblasts (HOB)
HOB Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12720
C-12760

1x106 cells in RNAlater

C-14071

1 Human Primary Cells

35

1.12 Preadipocytes

Proliferation/differentiation
Physiological

studies
and pathological

research
metabolism research
Obesity/diabetes studies
Oncology (e.g. breast cancer)
Fat

Features
From

subcutaneous and visceral


adipose tissue
Available from different locations
Cryopreserved at second passage
Differentiation capacity to adipocytes
Growth and Differentiation Media
available
Human White Preadipocytes (HWP) are
isolated from adult subcutaneous or visceral adipose tissue from different locations (lot specific source information is
available on request).

Adipose tissue is crucial in energy storage


and metabolic homeostasis. An increase
in adipose tissue results either from an
enlargement of mature adipocytes or
from the differentiation of adipocyte
precursor cells (preadipocytes) into new
mature adipocytes. These preadipocytes
are already present in adipose tissues.
They can proliferate throughout adult
life and replace the cells that have differentiated into mature adipocytes.
Since this differentiation process is controlled by a variety of growth factors and
hormones, preadipocytes are suitable
for the investigation of the physiological mechanisms controlling proliferation,
differentiation, and function of adipose
tissue.

Recommended media & reagents


Preadipocyte Growth Medium
(see page 77)
Preadipocyte Differentiation Medium
(see page 77)
Adipocyte Nutrition Medium
(see page 77)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Related products
subcutaneous Pellet
(see page 92)
HWP visceral Pellet (see page 92)
PromoFectin Transfection Reagent
(see page 157)
HWP

The PromoCell adipocyte cell culture


system provides preadipocytes with optimized growth media and serum-free
differentiation media.

Fig. 1: Left: Undifferentiated HWP (phase contrast). Middle: HWP after in vitro differentiation into adipocytes using the PromoCell
Preadipocyte Differentiation Medium (phase contrast). Right: HWP after in vitro differentiation into adipocytes (Lipid droplets
stained with Oil Red O).

https://www.promocell.com/shop
Product
Human White Preadipocytes (HWP)
subcutaneous
HWP subcutaneous Pellet
Human White Preadipocytes (HWP)
visceral
HWP visceral Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12730
C-12731

1x106 cells in RNAlater

C-14072

500,000 cryopreserved cells


500,000 proliferating cells

C-12732
C-12733

1x106 cells in RNAlater

C-14073

Human Primary
Cells

Applications

36

1 Human Primary Cells

1.13 Skeletal Muscle Cells


Applications

Human Primary
Cells

Gene therapy research


Transplantation research
Developmental/differentiation
studies
Tissue repair research
Pharmacological testing
Neuromuscular disease research

Features

liferate and fuse with damaged muscle


fibers or with one another forming new
myotubes.
SkMC are optimal for in vitro muscle
studies. They proliferate very well in
the mitogen-rich PromoCell Skeletal
Muscle Cell Growth Medium. Fusion to
myotubes with typical multinucleated
syncytia can be induced by using the
PromoCell Skeletal Muscle Cell Differentiation Medium.

Recommended media & reagents


Skeletal Muscle Cell Growth Medium
(see page 78)
Skeletal Muscle Cell Differentiation
Medium (see page 78)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Related products
Pellet (see page 92)
PromoFectin Transfection Reagent
(see page 157)
SkMC

Sarcomeric myosin positive


Available from different muscles
Differentiation capacity to multi nucleate syncytia
Growth and Differentiation Media
available
Human Skeletal Muscle Cells (SkMC) are
isolated from different skeletal muscles
(e.g. Musculus pectoralis major) from
adult single donors (lot specific source
information is available on request).
They are positive for sarcomeric myosin
and negative for smooth muscle specific
-actin.
New skeletal muscle cells originate from
quiescent satellite cells, which are located in the muscle fibers between the basal
lamina and the sarcolemma. Quiescent
satellite cells are activated by stimuli such
as muscle damage. After activation, the
cells, now called myoblasts, start to pro-

Fig. 1: SkMC culture in phase contrast.

Fig. 2: Differentiated SkMC culture in


phase contrast. Arrows indicate multinucleated syncytia.

For more information please see


www.promocell.com/knowledge-base

https://www.promocell.com/shop
Product
Human Skeletal Muscle Cells (SkMC)
SkMC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12530
C-12580

1x106 cells in RNAlater

C-14060

1 Human Primary Cells

37

1.14 Smooth Muscle Cells

Organ

specific drug testing


matrix interaction

Cell-extracellular

studies
Physiological

and biochemical studies

Oncology
Pathological

studies
(e.g. hypertension)
Atherosclerosis research

the walls of blood vessels and hollow organs like the bladder, the uterus, and the
gastrointestinal tract and are responsible
for involuntary movements like peristaltic contractions, which propel food along
the gastrointestinal tract.
PromoCell Smooth Muscle Cells are
available from the aorta, the coronary
artery, the pulmonary artery, the umbilical artery, the trachea, the bronchi, the
bladder, the prostate, and the uterus.

Fig. 1: HTSMC culture in phase contrast.


Human Primary
Cells

Applications

Features
muscle -actin positive
CD90 negative
Routinely morphology tested
Cryopreserved at second passage
Smooth

Smooth muscle cells, which form nonstriated muscles, are structurally and
functionally different from skeletal and
cardiac muscle cells. They are located in

Available Smooth Muscle Cells:


HAoSMC (see below)
HCASMC (see page 38)
HPASMC (see page 38)
HUASMC (see page 39)
HTSMC (see page 39)
HBSMC (see page 40)
HBdSMC (see page 40)
HPrSMC (see page 41)
HUtSMC (see page 41)

Fig. 2: HTSMC culture stained for smooth


muscle -actin. Nuclei counterstained
with DAPI.

Human Aortic Smooth Muscle


Cells (HAoSMC)
HAoSMC are isolated from plaque-free
regions of the human aorta (see Fig. 3)
and stain positive for smooth muscle
-actin. The pulsatile pressure produced
by the heart causes a cyclic distention
of the aorta. The smooth muscle cells in
the aortic wall subsequently contract it
again. Changes in the arterial wall, associated with vascular diseases such as atherosclerosis and hypertension, strongly
influence this process.

Tunica externa
(Connective tissue)

Tunica media
(Smooth muscle cells)

HAoSMC are suitable for studying the


role of smooth muscle cells under normal
or disease conditions in vitro.

Tunica intima
(Endothelial cells)

Fig. 3: Diagram of a blood vessel.


Recommended media & reagents
Smooth Muscle Cell Growth
Related products
HAoSMC Pellet (see page 91)
Medium 2 (see page 79)
DetachKit (see page 95)
Human Aortic Adventitial Fibroblasts
Cryo-SFM (see page 79) (see page 28)

Human

Aortic Endothelial Cells


(see page 15)
PromoFectin Transfection Reagent
(see page 157)

https://www.promocell.com/shop
Product
Human Aortic Smooth Muscle Cells
(HAoSMC)
HAoSMC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12533
C-12532

1x106 cells in RNAlater

C-14053

38

1 Human Primary Cells

Human Coronary Artery Smooth


Muscle Cells (HCASMC)
Left main coronary
artery

Human Primary
Cells

HCASMC are isolated from human coronary arteries (see Fig. 4) and stain positive for smooth muscle -actin. Coronary arteries supply the heart with blood
and obstruction in atherosclerosis causes
myocardial infarction.

Circumflex coronary
artery

HCASMC are qualified to study the


function of smooth muscle cells in the
development of atherosclerotic plaques
or in other diseases like hypertension.
They are also useful for stent-graft compatibility testing.

Right coronary
artery

Left anterior descending


coronary artery

Fig. 4: Location of the coronary arteries.


Recommended media & reagents
Smooth Muscle Cell Growth
Medium 2 (see page 79)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Related products
Pellet (see page 91)
Human Coronary Artery Endothelial
Cells (see page 15)
HCASMC

PromoFectin

Transfection Reagent
(see page 157)

https://www.promocell.com/shop
Product
Human Coronary Artery Smooth Muscle
Cells (HCASMC)
HCASMC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12511
C-12512

1x106 cells in RNAlater

C-14052

Human Pulmonary Artery Smooth


Muscle Cells (HPASMC)

DetachKit

HPASMC are isolated from human pulmonary arteries and stain positive for
smooth muscle -actin. They are suited
for studying the function of pulmonary
arteries under normal and disease conditions (e.g. vasoconstriction induced
by alveolar hypoxia). They are also useful for physiological studies of vascular
smooth muscle cells.

Related products
Pellet (see page 92)
Human Pulmonary Artery Endothelial
Cells (see page 16)
PromoFectin Transfection Reagent
(see page 157)

Recommended media & reagents


Smooth Muscle Cell Growth
Medium 2 (see page 79)

Cryo-SFM

(see page 95)


(see page 79)

HPASMC

Fig. 5: Top: HPASMC culture in phase


contrast. Bottom: HPASMC culture
stained for smooth muscle -actin.
Nucleus counterstained with DAPI.
https://www.promocell.com/shop

Product
Human Pulmonary Artery Smooth Muscle
Cells (HPASMC)
HPASMC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12521
C-12522

1x106 cells in RNAlater

C-14051

1 Human Primary Cells

HUASMC are isolated from the arteries


of the umbilical cord and stain positive
for smooth muscle -actin. Physiological
and biochemical processes of vascular
diseases can be analyzed with HUASMC
in culture.

Recommended media & reagents


Smooth Muscle Cell Growth
Medium 2 (see page 79)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Related products
HUASMC Pellet (see page 92)
Human Umbilical Artery Endothelial
Cells (see page 14)
PromoFectin Transfection Reagent
(see page 157)

https://www.promocell.com/shop
Product
Human Umbilical Artery Smooth Muscle
Cells (HUASMC)
HUASMC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12500
C-12550

1x106 cells in RNAlater

C-14050

Human Tracheal Smooth Muscle


Cells (HTSMC)

Paries
membranaceus

HTSMC are isolated from the trachea


(see Fig. 6) from single donors and stain
positive for smooth muscle -actin. They
connect the endings of the tracheal
cartilage by a contractile bridge called
the paries membranaceus and consequently regulate the airway lumen. Under pathological conditions like asthma,
the amount of smooth muscle cells in
the trachea walls is increased leading to
smooth muscle hypertrophy or hyperplasia. Hence, HTSMC are suitable for in
vitro studies of asthma and other pulmonary diseases.
Recommended media & reagents
Smooth Muscle Cell Growth
Medium 2 (see page 79)
DetachKit (see page 95)
Cryo-SFM (see page 79)
Related products
Pellet (see page 92)

HTSMC

Hyaline cartilage
Mucosa

Fig. 6: Cross section through the trachea with c-shaped cartilage connected by the
tracheal muscles.
Human

Tracheal Epithelial Cells


(see page 23)
PromoFectin Transfection Reagent
(see page 157)

https://www.promocell.com/shop
Product
Human Tracheal Smooth Muscle Cells
(HTSMC)
HTSMC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12565
C-12566

1x106 cells in RNAlater

C-14056

Human Primary
Cells

Human Umbilical Artery Smooth


Muscle Cells (HUASMC)

39

40

1 Human Primary Cells

Human Primary
Cells

Human Bronchial Smooth Muscle


Cells (HBSMC)
HBSMC are isolated from the bronchi
from single donors and stain positive for
smooth muscle -actin. They connect
the endings of the bronchial cartilage
and consequently regulate the airway lumen. Under pathological conditions like
asthma, the amount of smooth muscle
cells in the bronchi walls is increased

leading to smooth muscle hypertrophy


or hyperplasia. Hence, HBSMC are suitable for in vitro studies of asthma and
other pulmonary diseases.
Recommended media & reagents
Smooth Muscle Cell Growth
Medium 2 (see page 79)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Related products
Pellet (see page 91)
Human Bronchial Epithelial Cells
(see page 24)
PromoFectin Transfection Reagent
(see page 157)
HBSMC

https://www.promocell.com/shop
Product
Human Bronchial Smooth Muscle Cells
(HBSMC)
HBSMC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12561
C-12562

1x106 cells in RNAlater

C-14055

Human Bladder Smooth Muscle


Cells (HBdSMC)
HBdSMC are isolated from the detrusor
muscle from the urinary bladder (see
Fig. 7) of single donors and stain positive for smooth muscle -actin. The thick
bladder wall contains smooth muscle cells
arranged in muscle bundles, which empty
the bladder by coordinated contraction.
HBdSMC can be used for in vitro studies
of physiological processes as well as the
effect of drugs on the bladder.
Recommended media & reagents
Smooth Muscle Cell Growth
Medium 2 (see page 79)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Mucosa
Submucosa

Detrusor Muscle

Adventitia

Fig. 7: Diagram of the bladder.

Related products
Pellet (see page 91)
Human Bladder Microvascular
Endothelial Cells (see page 20)
PromoFectin Transfection Reagent
(see page 157)
HBdSMC

https://www.promocell.com/shop
Product
Human Bladder Smooth Muscle Cells
(HBdSMC)
HBdSMC Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12571
C-12572

1x106 cells in RNAlater

C-14057

1 Human Primary Cells

41

Human Prostate Smooth Muscle


Cells (HPrSMC)
HPrSMC are isolated from the prostate
from single donors and stain positive
for smooth muscle a-actin. The prostate produces secretions that get into
the urethra through contraction of the
smooth muscle cells.
Prostate smooth muscle cells can be involved in prostitis and benign prostatic
hyperplasia (BPH). HPrSMC can be used
to study these prostate diseases or physiological processes in vitro.
Recommended media & reagents
Smooth Muscle Cell Growth
Medium 2 (see page 79)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Lateral lobes
Capsule
Human Primary
Cells

Verumontanum
Ejaculatory duct

Fig. 8: Diagram of the prostate.


PromoFectin

Transfection Reagent
(see page 157)

Related products
HPrSMC Pellet (see page 92)

https://www.promocell.com/shop
Product
Human Prostate Smooth Muscle Cells
(HPrSMC)

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12573
C-12574

1x106 cells in RNAlater

C-14058

HPrSMC Pellet

Human Uterine Smooth Muscle


Cells (HUtSMC)
HUtSMC are isolated from the myometrium (the middle layer of the uterine wall,
see Fig. 9) and stain positive for smooth
muscle -actin. During pregnancy, hormones and growth factors cause an extensive hypertrophy of uterine smooth
muscle cells.
This and other morphological and biochemical effects on the uterus can be
studied in vitro using HUtSMC.
Recommended media & reagents
Smooth Muscle Cell Growth
Medium 2 (see page 79)
DetachKit (see page 95)
Cryo-SFM (see page 79)

Uterine tube
Perimetrium (peritoneum)
Myometrium (muscle)

Uterine cavity

Endometrium (epithelium)
Cavity of cervix

Vagina
Fig. 9: Diagram of the uterus.
Human

Uterine Microvascular
Endothelial Cells (see page 21)

Related products
HUtSMC Pellet (see page 92)
Fig. 9: Diagram of the uterus.

Product
Human Uterine Smooth Muscle Cells
(HUtSMC)
HUtSMC Pellet

https://www.promocell.com/shop
Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12575
C-12576

1x106 cells in RNAlater

C-14059

42

1 Human Primary Cells

Overview Human Primary Cells

Population
Doublings

Page

Sarcomeric -actinin+
Slow muscle myosin+

> 15

11

P2

Differentiation tested

> 10

12

5,000 - 10,000
cells per cm

P1

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

13

C-22010
C-22011

5,000 - 10,000
cells per cm

P1

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

13

C-12206

C-22011

5,000 - 10,000
cells per cm

P1

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

13

Human Umbilical Vein


Endothelial Cells (HUVEC)
isolated in Growth Medium 2,
pooled

C-12208

C-22011

5,000 - 10,000
cells per cm

P1

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

13

Human Umbilical Vein


Endothelial Cells (HUVEC)
pre-screened

C-12205

C-22010
C-22011

5,000 - 10,000
cells per cm

P1

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

13

Human Umbilical Artery


Endothelial Cells (HUAEC)

C-12202

C-22010
C-22011

5,000 - 10,000
cells per cm

P1

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

14

Human Aortic
Endothelial Cells (HAoEC)

C-12271

C-22020
C-22022

5,000 - 10,000
cells per cm

P2

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

15

Human Coronary Artery


Endothelial Cells (HCAEC)

C-12221

C-22020
C-22022

5,000 - 10,000
cells per cm

P2

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

15

Human Pulmonary Artery


Endothelial Cells (HPAEC)

C-12241

C-22010
C-22011

5,000 - 10,000
cells per cm

P2

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

16

Human Saphenous Vein


Endothelial Cells (HSaVEC)

C-12231

C-22010
C-22011

5,000 - 10,000
cells per cm

P2

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

16

Human Dermal Microvascular


Endothelial Cells (HDMEC)
juvenile foreskin

C-12210

C-22020
C-22022

10,000 - 20,000
cells per cm

P2

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

17

Human Dermal Microvascular


Endothelial Cells (HDMEC)
adult donor

C-12212

C-22020
C-22022

10,000 - 20,000
cells per cm

P2

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

17

Human Dermal Microvascular


Endothelial Cells (HDMEC)
pre-screened

C-12215

C-22020
C-22022

10,000 - 20,000
cells per cm

P2

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

17

Human Dermal Blood


Endothelial Cells (HDBEC)
juvenile foreskin

C-12211

C-22020

10,000 - 20,000
cells per cm

P2

PodoplaninProx-1CD31+

> 15

18

Human Dermal Blood


Endothelial Cells (HDBEC)
adult donor

C-12225

C-22020

10,000 - 20,000
cells per cm

P2

PodoplaninProx-1CD31+

> 15

18

Human Dermal Lymphatic


Endothelial Cells (HDLEC)
juvenile foreskin

C-12216

C-22022

10,000 - 20,000
cells per cm

P2

Podoplanin+
Prox-1+
CD31+

> 15

19

Human Dermal Lymphatic


Endothelial Cells (HDLEC)
adult donor

C-12217

C-22022

10,000 - 20,000
cells per cm

P2

Podoplanin+
Prox-1+
CD31+

> 15

19

Human Bladder Microvascular


Endothelial Cells (HBdMEC)

C-12291

C-22020
C-22022

10,000 - 20,000
cells per cm

P2

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

20

Human Primary
Cells

Product

Cat. No.
Cryopr. Cells

Recommended
Culture Media*

Plating Density

Passage after
Thawing

Human Cardiac Myocytes


(HCM)

C-12810

C-22070

10,000 - 15,000
cells per cm

P2

Human Chondrocytes (HCH)

C-12710

C-27101

10,000 - 20,000
cells per cm

Human Umbilical Vein


Endothelial Cells (HUVEC)
single donor

C-12200

C-22010
C-22011

Human Umbilical Vein


Endothelial Cells (HUVEC)
pooled

C-12203

Human Umbilical Vein


Endothelial Cells (HUVEC)
isolated in Growth Medium 2,
single donor

* The media in this table are ready-to-use. For Media Kits see page 60.

Marker

43

Population
Doublings

Page

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

20

P2

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

21

10,000 - 20,000
cells per cm

P2

vWF+
CD31+
Dil-Ac-LDL uptake+

> 15

21

C-22022

10,000 - 20,000
cells per cm

P2

vWF+
CD31+
Dil-Ac-LDL uptake+

> 10

22

C-12620

C-21060

10,000 - 15,000
cells per cm

P2

Cytokeratin+

> 15

23

Human Tracheal
Epithelial Cells (HTEpC)

C-12644

C-21060

10,000 - 15,000
cells per cm

P2

Cytokeratin+

> 15

23

Human Bronchial
Epithelial Cells (HBEpC)

C-12640

C-21060

10,000 - 15,000
cells per cm

P2

Cytokeratin+

> 15

24

Human Small Airway


Epithelial Cells (HSAEpC)

C-12642

C-21070

10,000 - 15,000
cells per cm

P2

Cytokeratin+

> 15

24

Human Mammary
Epithelial Cells (HMEpC)

C-12650

C-21010

10,000 - 15,000
cells per cm

P2

Cytokeratin+

> 15

25

Human Renal
Epithelial Cells (HREpC)

C-12665

C-26001
C-26030

10,000 - 15,000
cells per cm

P2

Cytokeratin+

> 15

25

Human Renal Cortical


Epithelial Cells (HRCEpC)

C-12660

C-26001
C-26030

10,000 - 15,000
cells per cm

P2

Cytokeratin+

> 15

26

Human Placental
Epithelial Cells (HPlEpC)

C-12395

C-26040

10,000 - 15,000
cells per cm

P2

Cytokeratin+

>5

26

Normal Human Dermal


Fibroblasts (NHDF)
juvenile foreskin

C-12300

C-23010

3,500 - 7,000
cells per cm

P2

CD90+

> 15

27

Normal Human Dermal


Fibroblasts (NHDF)
adult donor

C-12302

C-23020

3,500 - 7,000
cells per cm

P2

CD90+

> 15

27

Human Pulmonary
Fibroblasts (HPF)

C-12360

C-23020

3,500 - 7,000
cells per cm

P2

CD90+

> 15

28

Human Aortic Adventitial


Fibroblasts (HAoAF)

C-12380

C-23020

3,500 - 7,000
cells per cm

P2

CD90+

> 10

28

Human Cardiac
Fibroblasts (HCF)

C-12375

C-23025

3,500 - 7,000
cells per cm

P2

CD90+

> 15

28

Human Uterine
Fibroblasts (HUF)

C-12385

C-23020

3,500 - 7,000
cells per cm

P2

CD90+

> 15

29

Human Villous Mesenchymal


Fibroblasts (HVMF)

C-12390

C-23020

3,500 - 7,000
cells per cm

P2

CD90+

> 15

29

Human Follicle Dermal


Papilla Cells (HFDPC)

C-12071

C-26501

5,000 - 10,000
cells per cm

P2

Alkaline
phosphatase+

> 10

30

Human Hepatocytes (HHpC)

C-12850

C-25010
C-25020

150,000
cells per cm

P0

Phase I reaction+
Phase II reaction+
Albumin+

n/a

31

Normal Human Epidermal


Keratinocytes (NHEK)
juvenile foreskin, single donor

C-12001

C-20011

5,000
cells per cm

P2

Cytokeratin+

> 15

32

Normal Human Epidermal


Keratinocytes (NHEK)
juvenile foreskin, pooled

C-12005

C-20011

5,000
cells per cm

P2

Cytokeratin+

> 15

32

Normal Human Epidermal


Keratinocytes (NHEK)
adult, single donor

C-12003

C-20011

5,000
cells per cm

P2

Cytokeratin+

> 15

32

Product

Cat. No.
Cryopr. Cells

Recommended
Culture Media*

Plating Density

Passage after
Thawing

Human Cardiac Microvascular


Endothelial Cells (HCMEC)

C-12285

C-22020
C-22022

10,000 - 20,000
cells per cm

P2

Human Pulmonary
Microvascular
Endothelial Cells (HPMEC)

C-12281

C-22020
C-22022

10,000 - 20,000
cells per cm

Human Uterine Microvascular


Endothelial Cells (HUtMEC)

C-12295

C-22020
C-22022

Human Brain Microvascular


Endothelial Cells (HBMEC)

C-12287

Human Nasal
Epithelial Cells (HNEpC)

* The media in this table are ready-to-use. For Media Kits see page 60.

Marker

Human Primary
Cells

1 Human Primary Cells

44

1 Human Primary Cells

Population
Doublings

Page

Cytokeratin+

> 15

32

P2

Mel-5+

> 15

33

5,000 - 10,000
cells per cm

P2

Mel-5+

> 15

33

C-24300

5,000 - 10,000
cells per cm

P2

Mel-5+

> 15

33

C-12720

C-27001
C-27020

10,000 - 20,000
cells per cm

P2

Alkaline
phosphatase+
Mineralization tested

> 10

34

Human White Preadipocytes


(HWP) subcutaneous

C-12730

C-27410
C-27436
C-27438

5,000
cells per cm

P2

Differentiation
tested

> 10

35

Human White Preadipocytes


(HWP) visceral

C-12732

C-27410
C-27436
C-27438

5,000
cells per cm

P2

Differentiation
tested

> 10

35

Human Skeletal Muscle Cells


(SkMC)

C-12530

C-23060
C-23061

3,500 - 7,000
cells per cm

P2

Sarcomeric myosin+
Differentiation tested

> 15

36

Human Aortic Smooth Muscle


Cells (HAoSMC)

C-12533

C-22062

7,500 - 10,000
cells per cm

P2

Smooth muscle
-actin+

> 15

37

Human Coronary Artery


Smooth Muscle Cells (HCASMC)

C-12511

C-22062

7,500 - 10,000
cells per cm

P2

Smooth muscle
-actin+

> 15

38

Human Pulmonary Artery


Smooth Muscle Cells (HPASMC)

C-12521

C-22062

7,500 - 10,000
cells per cm

P2

Smooth muscle
-actin+

> 15

38

Human Umbilical Artery


Smooth Muscle Cells (HUASMC)

C-12500

C-22062

7,500 - 10,000
cells per cm

P2

Smooth muscle
-actin+

> 15

39

Human Tracheal
Smooth Muscle Cells (HTSMC)

C-12565

C-22062

7,500 - 10,000
cells per cm

P2

Smooth muscle
-actin+

> 15

39

Human Bronchial
Smooth Muscle Cells (HBSMC)

C-12561

C-22062

7,500 - 10,000
cells per cm

P2

Smooth muscle
-actin+

> 15

40

Human Bladder
Smooth Muscle Cells (HBdSMC)

C-12571

C-22062

7,500 - 10,000
cells per cm

P2

Smooth muscle
-actin+

> 15

40

Human Prostate
Smooth Muscle Cells (HPrSMC)

C-12573

C-22062

7,500 - 10,000
cells per cm

P2

Smooth muscle
-actin+

> 15

41

Human Uterine
Smooth Muscle Cells (HUtSMC)

C-12575

C-22062

7,500 - 10,000
cells per cm

P2

Smooth muscle
-actin+

> 15

41

Human Primary
Cells

Product

Cat. No.
Cryopr. Cells

Recommended
Culture Media*

Normal Human Epidermal


Keratinocytes (NHEK)
adult, pooled

C-12006

C-20011

5,000
cells per cm

P2

Normal Human Epidermal


Melanocytes (NHEM)
juvenile foreskin

C-12400

C-24010

5,000 - 10,000
cells per cm

Normal Human Epidermal


Melanocytes (NHEM) juvenile
foreskin, cultured in M2 Medium

C-12402

C-24300

Normal Human Epidermal


Melanocytes (NHEM) adult
donor, cultured in M2 Medium

C-12403

Human Osteoblasts (HOB)

* The media in this table are ready-to-use. For Media Kits see page 60.

Plating Density

Passage after
Thawing

Marker

1 Human Primary Cells

45

Human Primary Cells by Tissue Type

Page

Human Mesenchymal Stem Cells


from Adipose Tissue (hMSC-AT)

49

Human White Preadipocytes (HWP)


subcutaneous

35

Human White Preadipocytes (HWP)


visceral

35

n Bladder
Human Bladder Microvascular
Endothelial Cells (HBdMEC)

20

Human Bladder
Smooth Muscle Cells (HBdSMC)

40

n Blood/Bone Marrow
Human Mesenchymal Stem Cells
from Bone Marrow (hMSC-BM)

49

Human Mononuclear Cells from Peripheral


Blood (hMNC-PB) single donor, ultra-pure

56

Human Mononuclear Cells from Peripheral


Blood (hMNC-PB) pooled, ultra-pure

56

Human Mononuclear Cells from Cord


Blood (hMNC-CB) single donor, ultra-pure

57

Human Mononuclear Cells from


Cord Blood (hMNC-CB) pooled, ultra-pure

57

Human CD34 Progenitor Cells from


Cord Blood (hCD34+-CB) single donor

52

Human CD34+ Progenitor Cells from


Cord Blood (hCD34+-CB) pooled

52

Human CD133 Progenitor Cells from


Cord Blood (hCD133+-CB) single donor

53

Human CD133+ Progenitor Cells from


Cord Blood (hCD133+-CB) pooled

53

Human CD14 Monocytes from Peripheral


Blood (hMoCD14+-PB), single donor

54

Human CD14+ Monocytes from Peripheral


Blood (hMoCD14+-PB), single donor,
pre-screened

54

Human Monocytes from Peripheral Blood


(hMo-PB), single donor
Human Monocytes from Peripheral Blood
(hMo-PB), single donor, pre-screened

Page

n Brain

n Adipose Tissue

Product

Human Brain Microvascular Endothelial


Cells (HBMEC)

22

n Heart
Human Cardiac Fibroblasts (HCF)

28

Human Cardiac Myocytes (HCM)

11

Human Cardiac Microvascular


Endothelial Cells (HCMEC)

20

n Kidney
Human Renal Epithelial Cells (HREpC)

25

Human Renal Cortical Epithelial Cells


(HRCEpC)

26

n Large Vessels
Human Aortic Adventitial
Fibroblasts (HAoAF)

28

Human Aortic Endothelial Cells (HAoEC)

15

Human Aortic
Smooth Muscle Cells (HAoSMC)

37

Human Coronary Artery


Endothelial Cells (HCAEC)

15

Human Coronary Artery


Smooth Muscle Cells (HCASMC)

38

Human Pulmonary Artery


Endothelial Cells (HPAEC)

16

Human Pulmonary Artery


Smooth Muscle Cells (HPASMC)

38

Human Umbilical Artery


Endothelial Cells (HUAEC)

14

Human Umbilical Artery


Smooth Muscle Cells (HUASMC)

39

54

Human Umbilical Vein


Endothelial Cells (HUVEC) single donor

13

54

Human Umbilical Vein


Endothelial Cells (HUVEC) pooled

13

Human Umbilical Vein Endothelial Cells


(HUVEC) isolated in Growth Medium 2,
single donor

13

Human Umbilical Vein Endothelial Cells


(HUVEC) isolated in Growth Medium 2,
pooled

13

n Bone/Joint/Skeletal System
Human Osteoblasts (HOB)

34

Human Chondrocytes (HCH)

12

Human Umbilical Vein


Endothelial Cells (HUVEC) pre-screened

13

Human Skeletal Muscle Cells (SkMC)

36

Human Saphenous Vein


Endothelial Cells (HSaVEC)

16

Human Primary
Cells

Product

46

1 Human Primary Cells

Product

Page

Human Primary
Cells

31

n Mammary Tissue
Human Mammary
Epithelial Cells (HMEpC)

Page

n Respiratory System

n Liver
Human Hepatocytes (HHpC)

Product

25

n Umbilical Cord
Human Umbilical Artery
Endothelial Cells (HUAEC)

14

Human Umbilical Artery


Smooth Muscle Cells (HUASMC)

39

Human Umbilical Vein


Endothelial Cells (HUVEC) single donor

13

Human Umbilical Vein


Endothelial Cells (HUVEC) pooled

13

Human Umbilical Vein Endothelial Cells


(HUVEC) isolated in Growth Medium 2,
single donor

13

Human Umbilical Vein Endothelial Cells


(HUVEC) isolated in Growth Medium 2,
pooled

13

Human Umbilical Vein


Endothelial Cells (HUVEC) pre-screened
Human Mesenchymal Stem Cells
from Umbilical Cord Matrix (hMSC-UC)

Human Nasal Epithelial Cells (HNEpC)

23

Human Tracheal Epithelial Cells (HTEpC)

23

Human Tracheal
Smooth Muscle Cells (HTSMC)

39

Human Bronchial Epithelial Cells (HBEpC)

24

Human Bronchial
Smooth Muscle Cells (HBSMC)

40

Human Small Airway


Epithelial Cells (HSAEpC)

24

Human Pulmonary Fibroblasts (HPF)

28

Human Pulmonary Microvascular


Endothelial Cells (HPMEC)

21

n Skin
Human Dermal Microvascular
Endothelial Cells (HDMEC) juvenile foreskin

17

Human Dermal Microvascular


Endothelial Cells (HDMEC) adult donor

17

13

Human Dermal Microvascular


Endothelial Cells (HDMEC) pre-screened

17

49

Human Dermal Blood


Endothelial Cells (HDBEC) juvenile foreskin

18

Human Dermal Blood


Endothelial Cells (HDBEC) adult donor

18

Human Dermal Lymphatic


Endothelial Cells (HDLEC) juvenile foreskin

19

Human Dermal Lymphatic


Endothelial Cells (HDLEC) adult donor

19

Normal Human Dermal


Fibroblasts (NHDF) juvenile foreskin

27

Normal Human Dermal


Fibroblasts (NHDF) adult donor

27

Human Follicle Dermal


Papilla Cells (HFDPC)

30

n Uterus
Human Uterine Fibroblasts (HUF)

29

Human Uterine Microvascular


Endothelial Cells (HUtMEC)

21

Human Uterine
Smooth Muscle Cells (HUtSMC)

41

n Placenta
Human Pericytes from Placenta (hPC-PL)

51

Normal Human Epidermal Keratinocytes


(NHEK) juvenile foreskin, single donor

32

Human Placental Epithelial Cells (HPlEpC)

26

Normal Human Epidermal Keratinocytes


(NHEK) juvenile foreskin, pooled

32

Human Villous Mesenchymal


Fibroblasts (HVMF)

29

Normal Human Epidermal Keratinocytes


(NHEK) adult, single donor

32

Normal Human Epidermal Keratinocytes


(NHEK) adult, pooled

32

Normal Human Epidermal


Melanocytes (NHEM) juvenile foreskin

33

Normal Human Epidermal Melanocytes (NHEM)


juvenile foreskin, cultured in M2 Medium

33

Normal Human Epidermal Melanocytes (NHEM)


adult donor, cultured in M2 Medium

33

n Prostate
Human Prostate
Smooth Muscle Cells (HPrSMC)

41

Human Stem and


Blood Cells
PromoCell offers a collection of adult stem cells, as well as
differentiated blood cells from normal human bone marrow,
umbilical cord tissue, placenta, adipose tissue, peripheral blood,
and cord blood. All cells are obtained from approved donor programs at associated medical centers. High-quality media that
simulate the in vivo environment as well as optimized differentiation media systems are also available for these cell types.

48 Content Chapter 2

Human Stem and Blood Cells


2.1 Mesenchymal Stem Cells . . . . . . . . . . . . . . 49
2.2 Pericytes . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
2.3 CD34+ Progenitor Cells . . . . . . . . . . . . . . . . 52
2.4 CD133+ Progenitor Cells . . . . . . . . . . . . . . . 53
2.5 Monocytes . . . . . . . . . . . . . . . . . . . . . . . . . 54
2.6 Mononuclear Cells . . . . . . . . . . . . . . . . . . . 56
Overview Human Stem and Blood Cells . . . . . . . . 58

2 Human Stem and Blood Cells

49

2.1 Mesenchymal Stem Cells

cell research
medicine research
Tissue engineering studies
Developmental experiments

Human Mesenchymal Stem Cells


from Bone Marrow (hMSC-BM)

Stem

Regenerative

Features
adult stem cells
for in vitro differentiation
Routinely analyzed by flow cytometry
Two Growth Media and five optmized
Differentiation Media available

PromoCell hMSC-BM are harvested from


normal human bone marrow. The cells
are tested for their ability to differentiate
in vitro into adipocytes, chondrocytes,
osteoblasts, and neuronal cells.

Multipotent
Tested

Mesenchymal Stem Cells (MSC), also


termed Mesenchymal Stromal Cells, are
multipotent cells that can differentiate
into a variety of cell types and have the
capacity for self renewal. MSC have
been shown to differentiate in vitro or
in vivo into adipocytes, chondrocytes,
osteoblasts, myocytes, neurons, hepatocytes, and pancreatic islet cells. Optimized PromoCell media are available to
support both the growth of MSC and
their differentiation into several different lineages. Recent experiments suggest that differentiation capabilities into
diverse cell types vary between MSC of
different origin.

Human Mesenchymal Stem Cells


from Umbilical Cord Matrix
(hMSC-UC)
PromoCell hMSC-UC are harvested from
normal human umbilical cord matrix
(Whartons jelly). The cells are tested for
their ability to differentiate in vitro into
adipocytes, chondrocytes, osteoblasts,
and neuronal cells.

Human Mesenchymal Stem Cells


from Adipose Tissue (hMSC-AT)
PromoCell hMSC-AT are harvested from
normal human adipose tissue. The cells
are tested for their ability to differentiate
in vitro into adipocytes, chondrocytes,
osteoblasts, and neuronal cells.

Recommended media & reagents


Stem Cell
Growth Medium (see page 80)
Mesenchymal Stem Cell
Growth Medium DXF (see page 80)
Mesenchymal Stem Cell Adipogenic
Differentiation Medium (see page 80)
Mesenchymal Stem Cell Chondrogenic
Differentiation Medium (see page 80)
Mesenchymal Stem Cell Chondrogenic
Differentiation Medium w/o Inducers
(see page 80)
Mesenchymal Stem Cell Osteogenic
Differentiation Medium (see page 80)
Mesenchymal Stem Cell Neurogenic
Differentiation Medium (see page 80)
DetachKit (see page 95)
Cell Dissociation Solution ACF
(see page 96)
Fibronectin Solution, human
(see page 99)
Mesenchymal

Related products
MSC-Qualified

Fetal Calf Serum


(see page 88)
hMSC-BM Pellet (see page 92)
hMSC-UC Pellet (see page 92)
hMSC-AT Pellet (see page 92)

For more information please see


www.promocell.com/knowledge-base

Fig. 1: Undifferentiated hMSC-BM cultured in MSC Growth Medium.

Fig. 2: Undifferentiated hMSC-BM cultured in MSC Growth Medium DXF.

Human Stem and


Blood Cells

Applications

Human Stem and


Blood Cells

50

2 Human Stem and Blood Cells

Fig. 3.1

Fig. 3.1: hMSC-BM after in vitro differentiation into adipocytes using PromoCell
Mesenchymal Stem Cell Adipogenic
Differentiation Medium (lipid droplets
stained with Oil Red O).
Fig. 3.2: hMSC-BM spheroid after in vitro
differentiation into cartilage using PromoCell
Mesenchymal Stem Cell Chondrogenic
Differentiation Medium (Alcian Blue
staining).

Fig. 3.2

Fig. 3.3: hMSC-BM after in vitro differentiation into osteoblasts using PromoCell
Mesenchymal StemCell Osteogenic Differentiation Medium (Alizarin Red S staining).
Fig. 3.4: hMSC-BM after in vitro differentiation into neuronal cells using PromoCell
Mesenchymal Stem Cell Neurogenic Differentiation Medium.
Fig. 3.3

Fig. 3.4

For additional stem cell related products, please visit


www.promokine.info/products/stem-cell-research

https://www.promocell.com/shop
Product
Human Mesenchymal Stem Cells
from Bone Marrow (hMSC-BM)
hMSC-BM Pellet
Human Mesenchymal Stem Cells
from Umbilical Cord Matrix (hMSC-UC)
hMSC-UC Pellet
Human Mesenchymal Stem Cells
from Adipose Tissue (hMSC-AT)
hMSC-AT Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12974
C-12975

1x106 cells in RNAlater

C-14090

500,000 cryopreserved cells


500,000 proliferating cells

C-12971
C-12972

1x106 cells in RNAlater

C-14091

500,000 cryopreserved cells


500,000 proliferating cells

C-12977
C-12978

1x106 cells in RNAlater

C-14092

2 Human Stem and Blood Cells

51

2.2 Pericytes
Applications
Angiogenesis
Regenerative

research
medicine research

Oncology

blood vessel walls. They are important


for angiogenesis, the structural integrity of the microvasculature, and blood
flow regulation. However, they can also
develop into malignant tumors called
hemangiopericytomas.

Non-hematopoietic

multipotent
mesenchymal-like cells
Important in angiogenesis
Routinely characterized by flow
cytometry
Isolated from human placenta
Pericytes are multipotent mesenchymallike cells found in association with small

To date, pericytes have demonstrated


the ability to differentiate into adipocytes, osteoblasts, fibroblasts, smooth
muscle cells, and phagocytes (macrophages).

Fig. 4: Proliferating human pericytes


(hPC-PL) from the placenta (phase contrast).

Recommended media & reagents


Pericyte Growth Medium
(see page 81)
DetachKit (see page 95)

Related products
hPC-PL Pellet (see page 92)

Count

CD146

Cell Lab Quanta SC TM, Beckman Coulter, Inc.

50

CD146+

25

Fig. 5: Flow cytometric analysis of PromoCell Pericytes.


> 98% of the cells are CD146+.

0
100

101

102

103

104

CD146

https://www.promocell.com/shop
Product
Human Pericytes from Placenta (hPC-PL)
hPC-PL Pellet

Size

Catalog Number

500,000 cryopreserved cells


500,000 proliferating cells

C-12980
C-12981

1x106 cells in RNAlater

C-14095

Human Stem and


Blood Cells

Features

52

2 Human Stem and Blood Cells

2.3 CD34+ Progenitor Cells


Applications
Stem

cell research
basic research
Hematological and immunological
studies
Vascular and angiogenesis research
Oncology research

Features
Multipotent

hematopoietic/
endothelial progenitors
CD34+ cell purity > 95%
Superior viability
CD34 is a glycosylated transmembrane
protein and represents a well-known

Recommended media & reagents


Hematopoietic Progenitor Cell
Expansion Medium DXF
(see page 81)
Hematopoietic Progenitor Medium
(see page 81)
Endothelial Progenitor Medium
(see page 82)
Related products
(see page 179)
SCF (see page 179)
flt-3 ligand (see page 179)
IL-3 (see page 179)
IL-6 CC (see page 179)
TPO (see page 179)
CD34 antibody
(see page 169)
G-CSF

Count

CD34

Cell Lab Quanta SC TM, Beckman Coulter, Inc.

Human Stem and


Blood Cells

Transplantation

marker for primitive blood- and bone


marrow-derived progenitor cells, especially for hematopoietic and endothelial
stem cells. Although the biological functions of CD34 are largely unknown, recent data suggest that CD34 is involved
in maintenance of the progenitor cells in
a phenotypically undifferentiated state.
PromoCell offers CD34+ Progenitor Cells
from the cord blood of healthy donors.
The CD34+ Progenitor Cells contain
two main cellular subpopulations, hematopoietic and endothelial progenitor
cells. Therefore, CD34+ Progenitor Cells
are suitable for a series of studies, e.g.
directed differentiation into more committed types of blood cells and endothelial lineages.

CD34+

50

25

Fig. 6: Flow cytometric analysis of PromoCell CD34+


Progenitor Cells. > 95% of the cells are CD34+.

0
0

10

102

103

104

CD34

https://www.promocell.com/shop
Product

Size

Catalog Number

Human CD34+ Progenitor Cells from Cord


Blood (hCD34+-CB), single donor

100,000 cryopreserved cells

C-12921

Human CD34+ Progenitor Cells


from Cord Blood (hCD34+-CB), pooled

100,000 cryopreserved cells

C-12924

2 Human Stem and Blood Cells

53

2.4 CD133+ Progenitor Cells

Hematology

research
cell research
Oncology
Stem

Features
progenitors, e.g. of hemato-
poietic lineages
Available from cord blood
CD133+ cell purity > 95%
Superior viability
Expansion and Differentiation Media
available

CD133 (AC133, Prominin-1) is a glycoprotein with sub-cellular localization in


plasma membrane protrusions. In humans, this protein was originally identified as an antigenic marker expressed
on hematopoietic stem cells. In addition,
CD133 is also expressed by neural and
muscle progenitor cells.

Primitive

CD133 cells are isolated from fresh cord


blood. These cells are highly proliferative
primitive progenitors. They can give rise
to mature terminally differentiated cells,
e.g. hematopoietic lineages, by means
of directed differentiation. PromoCell
also provides suitable expansion and differentiation media.
+

Recommended media & reagents


Hematopoietic Progenitor Cell
Expansion Medium DXF (see page 81)
Cytokine Mix E for HPC Expansion
Medium DXF (see page 81)
Hematopoietic Progenitor Medium
(see page 81)
Related products
G-CSF (see page 179)
SCF (see page 179)
flt-3 ligand (see page 179)
IL-3 CC (see page 179)
IL-6 CC (see page 179)
TPO (see page 179)
CD133 antibody
(see page 169)

103

CD133+ cells
102

10

Fig. 7: Flow cytometric analysis of cord blood-derived


CD133+ Progenitor Cells, demonstrating their high
purity.

0
0

10

102

Cell Lab Quanta SC TM, Beckman Coulter, Inc.

CD45

103

CD133

https://www.promocell.com/shop
Product

Size

Catalog Number

Human CD133+ Progenitor Cells from


Cord Blood (hCD133+-CB), single donor

100,000 cryopreserved cells

C-12927

Human CD133+ Progenitor Cells from


Cord Blood (hCD133+-CB), pooled

100,000 cryopreserved cells

C-12929

Human Stem and


Blood Cells

Applications

54

2 Human Stem and Blood Cells

2.5 Monocytes
Applications
Dendritic

Cell Research

(Auto-)Immunity
Infection

biology

Features
Isolated

Human Stem and


Blood Cells

from fresh peripheral blood


analyzed by flow cytometry
CD14+-selected as well as untouched
Monocytes available

gradient-based method completely


devoid of using any immunomagnetic
beads. The advantage of these completely native Monocytes is that potential effects of bound magnetic particles
(which can be ingested by this phagocytic cell type) are excluded.

Routinely

Monocytes (Mo) are immature phagocytic cells circulating in the blood. Acting
as antigen-presenting immune cells, they
can phagocytize and degrade microbes
and particulate matter. Monocytes can
differentiate into several different cell
types. The most common applications
for Monocytes are the in vitro differentiation into monocyte-derived dendritic
cells (moDC), macrophages, and osteoclasts as well as their use as phagocytic
immune cells in immunology and infection biology.
CD14 belongs to the family of LPS receptor antigens and is strongly expressed
on the majority of Monocytes. Thus,
binding of immunomagnetic particles
to this antigen is employed for purification of PromoCell CD14+-Monocytes
(hMoCD14+-PB) from Mononuclear
Cells (MNC).
In contrast, the untouched Monocytes (hMo-PB) provided by PromoCell
are enriched from MNC by a density

Human CD14+ Monocytes


(hMoCD14+-PB)
PromoCell Human CD14+ Monocytes
are isolated from fresh peripheral blood.
After isolation of the ultra-pure mononuclear cell fraction by proprietary methods, the CD14+ Monocytes are isolated
by positive selection with > 95% purity.
In addition, hMoCD14+-PB are available
which are pre-screened for their differentiation capacity into dendritic cells.

Recommended media & reagents


Mononuclear Cell Medium (see page 84)
DC Generation Medium (see page 82)
Related products
hMoCD14+-PB single donor Pellet
(see page 91)
hMoCD14+-PB single donor
pre-screened Pellet (see page 92)
hMo-PB single donor Pellet
(see page 92)
hMo-PB single donor
pre-screened Pellet (see page 92)
PromoFectin-Macrophage
Transfection Reagent
(see page 158)
G-CSF (see page 179)
GM-CSF (see page 179)
IL-4 CC (see page 179)
TNF-alpha (see page 179)
M-CSF (see page 179)
sRANKL (see page 179)
CD14 antibody
(see page 169)

Human Monocytes (hMo-PB)


PromoCell Human untouched Monocytes are isolated from fresh peripheral
blood. After isolation of the ultra-pure
mononuclear cell fraction by proprietary
methods, the Monocyte fraction is further purified to > 70% by an advanced
multi-gradient technique.
In addition, hMo-PB are available which
are pre-screened for their differentiation
capacity into dendritic cells.

Fig. 8: Cluster formation during


differentiation of Monocytes into moDC.

For more information please see


www.promocell.com/knowledge-base

Fig. 9: Mature moDC cultured in


PromoCell DC Generation Medium showing typical dendrite-like structures.

2 Human Stem and Blood Cells

55

Cell Lab Quanta SC TM, Beckman Coulter, Inc.

CD14+

CD14-

12,5

Fig. 10a: Flow cytometric analysis of day 8 mature


moDCs generated in the PromoCell DC Generation
Medium. More than 98% of the cells are negative for
CD14.

0
0

101

102

103

104

103

104

CD14

Count
Cell Lab Quanta SC TM, Beckman Coulter, Inc.

CD83+

CD83-

12,5

Fig. 10b: Flow cytometric analysis of day 8 mature


moDCs generated in the PromoCell DC Generation
Medium. The DC maturation marker CD83 is strongly
expressed by > 98% of the cells.

0
0

101

102

CD83

https://www.promocell.com/shop
Product

Size

Catalog Number

10 x 106 cryopreserved cells

C-12909

1x106 cells in RNAlater

C-14110

10 x 106 cryopreserved cells

C-12912

1x106 cells in RNAlater

C-14112

10 x 106 cryopreserved cells

C-12910

1x106 cells in RNAlater

C-14111

Human Monocytes from Peripheral Blood


(hMo-PB), single donor, pre-screened

10 x 106 cryopreserved cells

C-12913

hMo-PB single donor pre-screened Pellet

1x106 cells in RNAlater

C-14113

Human CD14+ Monocytes from Peripheral


Blood (hMoCD14+-PB), single donor
hMoCD14+-PB single donor Pellet
Human CD14+ Monocytes from Peripheral
Blood (hMoCD14+-PB), single donor,
pre-screened
hMoCD14+-PB single donor
pre-screened Pellet
Human Monocytes from Peripheral Blood
(hMo-PB), single donor
hMo-PB single donor Pellet

Human Stem and


Blood Cells

Count

56

2 Human Stem and Blood Cells

2.6 Mononuclear Cells


Applications
Stem

cell research
basic research
Hematological and immunological
studies
Oncology research
Virology and infection biology

Features
Routinely

analyzed by flow cytometry


content < 5%
Red blood cells depleted without lysis
High purity and viability
Available from single and pooled
donors
Granulocyte

matopoietic and endothelial progenitors,


monocytes, and lymphocyte subpopulations (see Fig. 12).

Human Mononuclear Cells from


Peripheral Blood (hMNC-PB)
Adult peripheral blood contains large
numbers of mature immune cells but
also some progenitor cells. Typical application fields for hMNC-PB from single
donors are immunology, oncology, hematology, virology, and infection biology.
PromoCell hMNC-PB pooled are preferably used for several types of infectious
virus tests, including neutralization assays. For HIV-related research, prior
CD8+ T-cell depletion is recommended.

Size [m]

Granulocytes
Monocytes
Cell Lab Quanta SC TM, Beckman Coulter, Inc.

16

14

Lymphocytes
12

Fig. 11: Flow cytometric analysis of Mononuclear


Cells from Cord Blood (hMNC-CB). Granulocyte
content 1%.

10
8
0
0

10

102

103

104

Side Scatter
CD45
104

CD34+ cells
103

Cell Lab Quanta SC TM, Beckman Coulter, Inc.

Human Stem and


Blood Cells

Transplantation

PromoCell Mononuclear Cells (MNC)


represent the lymphocyte and monocyte
fraction isolated from umbilical cord blood
or peripheral blood. They are prepared
by optimized low-density gradient centrifugation, which effectively reduces the
granulocyte content (see Fig. 11). Red
blood cells are gently but thoroughly depleted by proprietary techniques instead
of osmotic lysis. This results in ultra-pure
mononuclear cells with superior viability
and unchanged biological function. In
addition, the cells do not clump after
thawing, allowing for easy handling and
reproducible results.
The MNC can be used directly for experiments or as a starting material for the
isolation of several cell types, e.g., he-

102

10

Fig. 12: Flow cytometric determination of CD34+ cells


within the PromoCell Mononuclear Cells from Cord
Blood (hMNC-CB).

0
0

10

102

CD34

103

104

2 Human Stem and Blood Cells

hMNC-CB are isolated from umbilical


cord blood, which has a much higher
content of primitive progenitor cells compared to adult peripheral blood.
Recommended media & reagents
Mononuclear Cell Medium
(see page 84)
Hematopoietic Progenitor Medium
(see page 81)

Endothelial

Progenitor Medium
(see page 82)
Hematopoietic Progenitor Cell
Expansion Medium DXF (see page 81)
Cytokine Mix E for HPC Expansion
Medium DXF (see page 81)
Related products
single donor Pellet
(see page 91)
hMNC-CB single donor Pellet
(see page 91)
hMNC-PB pooled Pellet (see page 91)

hMNC-CB

pooled Pellet (see page 91)


Separation
Medium 1077 (see page 84)
GM-CSF (see page 179)
IL-4 CC (see page 179)
M-CSF (see page 179)
TNF-alpha (see page 179)
sRANKL (see page 179)
Lymphocyte

hMNC-PB

Human Stem and


Blood Cells

Human Mononuclear Cells from


Cord Blood (hMNC-CB)

57

https://www.promocell.com/shop
Product

Size

Catalog Number

25 x 106 cryopreserved cells

C-12907

1x106 cells in RNAlater

C-14098

25 x 10 cryopreserved cells

C-12908

1x106 cells in RNAlater

C-14099

Human Mononuclear Cells


from Cord Blood (hMNC-CB),
single donor, ultra-pure

25 x 10 cryopreserved cells

C-12901

hMNC-CB single donor Pellet

1x106 cells in RNAlater

C-14096

Human Mononuclear Cells


from Cord Blood (hMNC-CB),
pooled, ultra-pure

25 x 10 cryopreserved cells

C-12904

1x106 cells in RNAlater

C-14097

Human Mononuclear Cells


from Peripheral Blood (hMNC-PB),
single donor, ultra-pure
hMNC-PB single donor Pellet
Human Mononuclear Cells
from Peripheral Blood (hMNC-PB),
pooled, ultra-pure
hMNC-PB pooled Pellet

hMNC-CB pooled Pellet

* These differentiation media are applicable for CFU-assays for hematopoietic progenitor cells (LTC-IC assays) or Hill-assays for endothelial progenitor cells
(Hill et al. 2003, N Engl J Med 348: 593-600).

58

2 Human Stem and Blood Cells

Overview Human Stem and Blood Cells

Human Stem and


Blood Cells

Product

Cat. No.
Cryopr.
Cells

Recommended
Culture Media

Recommended
Differentiation
Media

Recommended
Sera

Plating
Density

Page

Human Mesenchymal Stem


Cells from Bone Marrow
(hMSC-BM)

C-12974

C-28010
C-28019

C-28011
C-28012
C-28013
C-28014
C-28015

C-37385
C-37386

4,000 cells
per cm2

49

Human Mesenchymal Stem


Cells from Umbilical Cord
Matrix (hMSC-UC)

C-12971

C-28010
C-28019

C-28011
C-28012
C-28013
C-28014
C-28015

C-37385
C-37386

4,000 cells
per cm2

49

Human Mesenchymal Stem


Cells from Adipose Tissue
(hMSC-AT)

C-12977

C-28010
C-28019

C-28011
C-28012
C-28013
C-28014
C-28015

C-37385
C-37386

4,000 cells
per cm2

49

Human Pericytes
from Placenta (hPC-PL)

C-12980

C-28040

C-37385
C-37386

4,000 cells
per cm2

51

Human CD34+ Progenitor Cells


from Cord Blood (hCD34+-CB),
single donor

C-12921

C-28021
C-39890

C-28020*
C-28025*

C-37387
C-37388

5,000 cells
per ml

52

Human CD34+ Progenitor Cells


from Cord Blood (hCD34+-CB),
pooled

C-12924

C-28021
C-39890

C-28020*
C-28025*

C-37387
C-37388

5,000 cells
per ml

52

Human CD133+ Progenitor Cells


from Cord Blood (hCD133+-CB),
single donor

C-12927

C-28021
C-39890

C-28020*

C-37387
C-37388

5,000 cells
per ml

53

Human CD133+ Progenitor Cells


from Cord Blood (hCD133+CB), pooled

C-12929

C-28021
C-39890

C-28020*

C-37387
C-37388

5,000 cells
per ml

53

Human CD14+ Monocytes


from Peripheral Blood
(hMoCD14+-PB), single donor

C-12909

C-28030

C-37387
C-37388

1 x106 cells
per ml

54

Human CD14+ Monocytes


from Peripheral Blood
(hMoCD14+-PB), single donor,
pre-screened

C-12912

C-28030

C-37387
C-37388

1 x106 cells
per ml

54

Human Monocytes from


Peripheral Blood (hMo-PB),
single donor

C-12910

C-28030

C-37387
C-37388

1 x106 cells
per ml

54

Human Monocytes from


Peripheral Blood (hMo-PB),
pre-screened

C-12913

C-28030

C-37387
C-37388

1 x106 cells
per ml

54

Human Mononuclear Cells


from Peripheral Blood (hMNCPB), single donor, ultra-pure

C-12907

C-28030

C-37387
C-37388

1 x106 cells
per ml

56

Human Mononuclear Cells


from Peripheral Blood (hMNCPB), pooled, ultra-pure

C-12908

C-28030

C-37387
C-37388

1 x106 cells
per ml

56

Human Mononuclear Cells


from Cord Blood (hMNC-CB),
single donor, ultra-pure

C-12901

C-28030
C-28021
C-39890

C-28020*
C-28025*

C-37387
C-37388

1 x106 cells
per ml

57

Human Mononuclear Cells


from Cord Blood (hMNC-CB),
pooled, ultra-pure

C-12904

C-28030
C-28021
C-39890

C-28020*
C-28025*

C-37387
C-37388

1 x106 cells
per ml

57

C-28050

C-28050

* These differentiation media are applicable for CFU-assays for hematopoietic progenitor cells (LTC-IC assays) or Hill-assays for endothelial
progenitor cells (Hill et al. 2003, N Engl J Med 348: 593-600).

Media and Sera


High-quality media and sera are vital for successful cell culture.
Therefore, PromoCell adheres to strict quality standards and
produces media and sera only from raw materials from strictly
approved sources. We also have invested a lot of effort in media optimization and have substituted serum by defined substances wherever possible. This ensures effective cell growth
and avoids the unpredictable effects of indeterminate compounds.

60 Content Chapter 3

Media and Sera


3.1

Media for Primary Cells . . . . . . . . . . . . . . 61

3.1.1

Myocyte Medium . . . . . . . . . . . . . . . . . . . . . 62

3.1.2

Chondrocyte Medium . . . . . . . . . . . . . . . . . . 62

3.1.3

Endothelial Cell Media . . . . . . . . . . . . . . . . . 63

3.1.4

Endothelial Cell Media MV . . . . . . . . . . . . . . 64

3.1.5

Epithelial Cell Media . . . . . . . . . . . . . . . . . . . 65

3.1.6

Fibroblast Media . . . . . . . . . . . . . . . . . . . . . . 70

3.1.7

Follicle Dermal Papilla Cell Medium . . . . . . . 72

3.1.8

Hepatocyte Media . . . . . . . . . . . . . . . . . . . . 73

3.1.9

Keratinocyte Medium . . . . . . . . . . . . . . . . . . 74

3.1.10 Melanocyte Media . . . . . . . . . . . . . . . . . . . . 75


3.1.11 Osteoblast Media . . . . . . . . . . . . . . . . . . . . . 76
3.1.12 Preadipocyte/Adipocyte Media . . . . . . . . . . . 77
3.1.13 Skeletal Muscle Cell Media . . . . . . . . . . . . . . 78
3.1.14 Smooth Muscle Cell Medium . . . . . . . . . . . . 79
3.1.15 Freezing Medium . . . . . . . . . . . . . . . . . . . . . 79

3.2

Media for Stem and Blood Cells . . . . . . . . . 80

3.2.1

Mesenchymal Stem Cell Media . . . . . . . . . . . 80

3.2.2

Pericyte Medium . . . . . . . . . . . . . . . . . . . . . 81

3.2.3

Hematopoietic Progenitor Cell Media . . . . . . 81

3.2.4

Endothelial Progenitor Medium . . . . . . . . . . 82

3.2.5

Dendritic Cell Generation Media . . . . . . . . . . 82

3.2.6

Macrophage Generation Media . . . . . . . . . . 83

3.2.7

Mononuclear Cell Medium . . . . . . . . . . . . . . 84

3.2.8

Blood Cell Separation Medium . . . . . . . . . . . 84

3.3

Classical Media . . . . . . . . . . . . . . . . . . . . . . 85

3.4

Insect Cell Culture Media . . . . . . . . . . . . . . 87

3.5 Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

3 Media and Sera

61

3.1 Media for Primary Cells


convenience or as Medium Kit, which
allows user control over the supplement
type and concentration.
Each medium lot is tested for its ability to
support the growth of the intended primary cells. In addition, biochemical and

Medium (Ready-to-use)

in culture (including L-Glutamine and


NaHCO3).
The shelf life of the Basal Medium is
9 months (at 4 to 8C), that of the
SupplementMix is 12 months (at -20C)
from the date of manufacture. After
adding the SupplementMix, the shelf life
of the complete medium is 6 weeks (at
4 to 8C).

The Medium (Ready-to-use) consists of


a 500 ml bottle with Basal Medium and
one vial with SupplementMix. Adding
the SupplementMix to the Basal Medium
results in the complete medium, which
contains all the growth factors and
supplements necessary for the propagation of the specific human primary cells

Medium Kit
The Medium Kit consists of a 500 ml
bottle with Basal Medium and a SupplementPack. The SupplementPack is a set
of individual vials with pre-measured
supplements. Adding all components of
the SupplementPack to the Basal Medium results in the complete medium,
which contains all the growth factors

sterility tests are performed*.


Note: The media for primary cells do not
contain antibiotics.

Media and Sera

The PromoCell media for primary cells


are formulated for the optimal growth
of specific types of primary human cells
and a variety of primary animal cells.
For most cell types, they are available
as Medium (Ready-to-use) for maximal

and supplements necessary for the propagation of specific human primary cells
in culture (including L-Glutamine and
NaHCO3). Since all supplements can be
added separately, the user has full control over the media formulation and can
adapt it to his/her experimental requirements.
The shelf life of the Basal Medium is
9 months (at 4 to 8C), and that of
the SupplementPack is 12 months
(at -20C) from the date of manufacture. After adding the supplements, the shelf life of the complete
medium is 6 weeks (at 4 to 8C).
Basal Media (with or without phenol
red) as well as SupplementMixes and
SupplementPacks can also be purchased
separately.

If you require special media modifications, we offer a Custom Media Service starting at 10 bottles per order.
Please ask for details.

*Certificates of analysis are available on request.

62

3 Media and Sera

Low Serum
3.1.1 Myocyte Medium
Myocyte Growth Medium
Recommended for
Cardiac Myocytes
(see page 11)

Media and Sera

Human

The PromoCell Myocyte Growth Medium


is a low serum (5% V/V) medium supplemented with well chosen hormones and
growth factors. It was developed for the
optimal expansion and maintenance of
primary cardiac myocytes in culture. The

medium is optimized for primary human


cells but can also be used for primary porcine cardiac myocytes.
Related products
Fetal Calf Serum (see page 87)

Final supplement concentrations


(after addition to the medium)
Fetal Calf Serum
Epidermal Growth Factor
(recombinant human)

0.05 ml / ml
0.5 ng / ml

Basic Fibroblast Growth Factor


(recombinant human)

2 ng / ml

Insulin (recombinant human)

5 g / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Myocyte Growth Medium (Ready-to-use)

500 ml

C-22070

Myocyte Growth Medium Kit

500 ml

C-22170

Myocyte Basal Medium

500 ml

C-22270

Myocyte Basal Medium, phenol red-free

500 ml

C-22275

Myocyte Growth Medium SupplementMix

for 500 ml

C-39275

Myocyte Growth Medium


SupplementPack

for 500 ml

C-39270

3.1.2 Chondrocyte Medium


Chondrocyte Growth Medium
Recommended for
Human

Chondrocytes (see page 12)

The PromoCell Chondrocyte Growth


Medium is designed for the optimal
cultivation of human chondrocytes. The
fetal calf serum in the medium is strictly
pretested for optimal performance.

The medium is optimized for primary


human cells but can also be used for primary porcine and rat chondrocytes.

Final supplement concentration


(after addition to the medium)
Fetal Calf Serum

0.1 ml / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Chondrocyte Growth Medium


(Ready-to-use)

500 ml

C-27101

Chondrocyte Basal Medium

500 ml

C-27111

Chondrocyte Basal Medium,


phenol red-free

500 ml

C-27115

for 500 ml

C-39635

Chondrocyte Growth Medium


SupplementMix

3 Media and Sera

63

Low Serum

Endothelial Cell Growth Medium


Endothelial Cell Growth Medium 2
Recommended for
Human

Umbilical Vein Endothelial


Cells (see page 13)
Human Umbilical Artery Endothelial
Cells (see page 14)
Human Pulmonary Artery Endothelial
Cells (see page 16)
Human Saphenous Vein Endothelial
Cells (see page 16)

Final supplement concentrations


(after addition to the medium)
Fetal Calf Serum

Endothelial Cell Growth Medium and


Endothelial Cell Growth Medium 2 are
low-serum (2% V/V) media optimized
for the cultivation of endothelial cells
from large blood vessels.
Endothelial Cell Growth Medium 2 lacks
Endothelial Cell Growth Supplement
(ECGS, bovine hypothalamic extract)
but contains Insulin-like Growth Factor
(Long R3 IGF) and Vascular Endothelial
Growth Factor (VEGF). Generally, VEGF
leads to higher endothelial cell proliferation in culture. But, because of its mul-

tiple effects on cell metabolism, it may


also interfere with certain experimental
setups. In these cases, we recommend
the use of Endothelial Cell Growth Medium, which contains no VEGF.
The media are optimized for primary
human cells but can also be used for
bovine, porcine, murine, caprine, rabbit,
elephant, dog, and rat endothelial cells
from large vessels as well as for various
endothelial cell lines.
Related products
Fetal Calf Serum (see page 87)

Endothelial Cell Growth Medium

Endothelial Cell Growth Medium 2

0.02 ml / ml

0.02 ml / ml

0.004 ml / ml

0.1 ng / ml

5 ng / ml

1 ng / ml

10 ng / ml

Insulin-like Growth Factor (Long R3 IGF)

20 ng / ml

Vascular Endothelial Growth Factor 165


(recombinant human)

0.5 ng / ml

Endothelial Cell Growth Supplement


Epidermal Growth Factor
(recombinant human)
Basic Fibroblast Growth Factor
(recombinant human)

Ascorbic Acid
Heparin
Hydrocortisone

1 g / ml

90 g / ml

22.5 g / ml

1 g / ml

0.2 g / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Endothelial Cell Growth Medium


(Ready-to-use)

500 ml

C-22010

Endothelial Cell Growth Medium Kit

500 ml

C-22110

Endothelial Cell Basal Medium

500 ml

C-22210

Endothelial Cell Basal Medium,


phenol red-free

500 ml

C-22215

Endothelial Cell Growth Medium


SupplementMix

for 500 ml

C-39215

Endothelial Cell Growth Medium


SupplementPack

for 500 ml

C-39210

Endothelial Cell Growth Medium 2


(Ready-to-use)

500 ml

C-22011

Endothelial Cell Growth Medium 2 Kit

500 ml

C-22111

Endothelial Cell Basal Medium 2

500 ml

C-22211

Endothelial Cell Basal Medium 2,


phenol red-free

500 ml

C-22216

Endothelial Cell Growth Medium 2


SupplementMix

for 500 ml

C-39216

Endothelial Cell Growth Medium 2


SupplementPack

for 500 ml

C-39211

Media and Sera

3.1.3 Endothelial Cell Media

64

3 Media and Sera

Low Serum
3.1.4 Endothelial Cell Media MV
Endothelial Cell Growth
Medium MV
Endothelial Cell Growth
Medium MV2

Media and Sera

Recommended for
Human

Dermal Microvascular
Endothelial Cells (see page 17)
Human Coronary Artery Endothelial
Cells (see page 15)
Human Aortic Endothelial Cells
(see page 15)
Human Pulmonary Microvascular
Endothelial Cells (see page 21)

Final supplement concentrations


(after addition to the medium)
Fetal Calf Serum
Endothelial Cell Growth Supplement
Epidermal Growth Factor
(recombinant human)
Basic Fibroblast Growth Factor
(recombinant human)

Human

Dermal Lymphatic Endothelial


Cells (see page 19)
Human Dermal Blood Endothelial
Cells (see page 18)
PromoCell Endothelial Cell Growth Medium MV and Endothelial Cell Growth
Medium MV2 were developed for the
in vitro cultivation of endothelial cells
from microvascular vessels, the coronary
artery, and the aorta.
Endothelial Cell Growth Medium MV2
lacks the Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic
extract) but contains the basic Fibroblast
Growth Factor, the Insulin-like Growth

Factor (Long R3 IGF-1) and the Vascular Endothelial Growth Factor (VEGF). Generally,
VEGF leads to higher endothelial cell proliferation in culture. But, because of its multiple
effects on the cell metabolism, it may also
interfere with certain experimental setups.
In these cases, we recommend the use of
Endothelial Cell Growth Medium MV.
The media are optimized for primary human cells but can also be used for bovine,
porcine, murine, rat, and dog endothelial
cells from microvascular vessels as well
as for various endothelial cell lines.
Related products
Fetal Calf Serum (see page 87)

Endothelial Cell Growth Medium MV

Endothelial Cell Growth Medium MV2

0.05 ml / ml

0.05 ml / ml

0.004 ml / ml

10 ng / ml

5 ng / ml

10 ng / ml

Insulin-like Growth Factor (Long R3 IGF-1)

20 ng / ml

Vascular Endothelial Growth Factor 165


(recombinant human)

0.5 ng / ml

Ascorbic Acid

1 g / ml

Heparin
Hydrocortisone

90 g / ml

1 g / ml

0.2 g / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Endothelial Cell Growth Medium MV


(Ready-to-use)

500 ml

C-22020

Endothelial Cell Growth Medium MV Kit

500 ml

C-22120

Endothelial Cell Basal Medium MV

500 ml

C-22220

Endothelial Cell Basal Medium MV,


phenol red-free

500 ml

C-22225

Endothelial Cell Growth Medium MV


SupplementMix

for 500 ml

C-39225

Endothelial Cell Growth Medium MV


SupplementPack

for 500 ml

C-39220

Endothelial Cell Growth Medium MV2


(Ready-to-use)

500 ml

C-22022

Endothelial Cell Growth Medium MV2 Kit

500 ml

C-22121

Endothelial Cell Basal Medium MV2

500 ml

C-22221

Endothelial Cell Basal Medium MV2,


phenol red-free

500 ml

C-22226

Endothelial Cell Growth Medium MV2


SupplementMix

for 500 ml

C-39226

Endothelial Cell Growth Medium MV2


SupplementPack

for 500 ml

C-39221

3 Media and Sera

65

Serum-Free
3.1.5 Epithelial Cell Media

Recommended for
Human

Nasal Epithelial Cells


(see page 23)
Human Bronchial Epithelial Cells
(see page 24)
Human Tracheal Epithelial Cells
(see page 23)

The PromoCell Airway Epithelial Cell


Growth Medium is a serum-free medium
optimized for the cultivation of epithelial
cells from large air passages.
The medium is optimized for primary
human cells but can also be used for
murine, porcine, equine, and rat airway
epithelial cells.

Media and Sera

Airway Epithelial Cell Growth


Medium

Final supplement concentrations


(after addition to the medium)
Bovine Pituitary Extract
Epidermal Growth Factor
(recombinant human)
Insulin (recombinant human)

0.004 ml / ml
10 ng / ml
5 g / ml

Hydrocortisone

0.5 g / ml

Epinephrine

0.5 g / ml

Triiodo-L-thyronine

6.7 ng / ml

Transferrin, holo (human)

10 g / ml

Retinoic Acid

0.1 ng / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Airway Epithelial Cell Growth Medium


(Ready-to-use)

500 ml

C-21060

Airway Epithelial Cell Growth Medium Kit

500 ml

C-21160

Airway Epithelial Cell Basal Medium

500 ml

C-21260

Airway Epithelial Cell Basal Medium,


phenol red-free

500 ml

C-21265

Airway Epithelial Cell Growth Medium


SupplementMix

for 500 ml

C-39165

Airway Epithelial Cell Growth Medium


SupplementPack

for 500 ml

C-39160

66

3 Media and Sera

Serum-Free
Small Airway Epithelial Cell
Growth Medium
Recommended for
Small Airway Epithelial Cells
(see page 24)

Media and Sera

Human

PromoCell Small Airway Epithelial Cell


Growth Medium is a serum-free medium
developed for the cultivation of epithelial
cells from the distal respiratory system.
The optimal adjustment of growth factors
in this medium ensures excellent in vitro
growth.

The medium is optimized for primary human cells but can also be used for porcine small airway epithelial cells.

Final supplement concentrations


(after addition to the medium)
Bovine Pituitary Extract
Epidermal Growth Factor
(recombinant human)
Insulin (recombinant human)

0.004 ml / ml
10 ng / ml
5 g / ml

Hydrocortisone

0.5 g / ml

Epinephrine

0.5 g / ml

Triiodo-L-thyronine

6.7 ng / ml

Transferrin, holo (human)

10 g / ml

Retinoic Acid

0.1 ng / ml

Bovine Serum Albumin-Fatty Acid Free


(BSA-FAF)

2.5 mg / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Small Airway Epithelial Cell Growth


Medium (Ready-to-use)

500 ml

C-21070

Small Airway Epithelial Cell Growth


Medium Kit

500 ml

C-21170

Small Airway Epithelial Cell Basal Medium

500 ml

C-21270

Small Airway Epithelial Cell Basal Medium,


phenol red-free

500 ml

C-21275

Small Airway Epithelial Cell Growth


Medium SupplementMix

for 500 ml

C-39175

Small Airway Epithelial Cell Growth


Medium SupplementPack

for 500 ml

C-39170

3 Media and Sera

67

Serum-Free
Mammary Epithelial Cell Growth
Medium
Recommended for

The PromoCell Mammary Epithelial Cell


Growth Medium is a serum-free medium
developed for the cultivation of epithelial
cells from the mammary gland.

The medium is optimized for primary human cells but can also be used for rat and
murine mammary epithelial cells as well
as mamma carcinoma cell lines.

Mammary Epithelial Cells


(see page 25)
Media and Sera

Human

Final supplement concentrations


(after addition to the medium)
Bovine Pituitary Extract
Epidermal Growth Factor
(recombinant human)
Insulin (recombinant human)
Hydrocortisone

0.004 ml / ml
10 ng / ml
5 g / ml
0.5 g / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Mammary Epithelial Cell Growth Medium


(Ready-to-use)

500 ml

C-21010

Mammary Epithelial Cell Growth Medium


Kit

500 ml

C-21110

Mammary Epithelial Cell Basal Medium

500 ml

C-21210

Mammary Epithelial Cell Basal Medium,


phenol red-free

500 ml

C-21215

Mammary Epithelial Cell Growth Medium


SupplementMix

for 500 ml

C-39115

Mammary Epithelial Cell Growth Medium


SupplementPack

for 500 ml

C-39110

68

3 Media and Sera

Serum-Free/Low Serum
Renal Epithelial Cell Growth
Medium
Renal Epithelial Cell Growth
Medium 2

Media and Sera

Recommended for
Human

Renal Epithelial Cells


(see page 25)
Human Renal Cortical Epithelial Cells
(see page 26)

Final supplement concentrations


(after addition to the medium)
Fetal Calf Serum
Epidermal Growth Factor
(recombinant human)
Insulin (recombinant human)
Epinephrine
Hydrocortisone

The PromoCell Renal Epithelial Cell


Growth Medium is a serum-free medium
for the cultivation of epithelial cells from
the kidneys.
The PromoCell Renal Epithelial Cell
Growth Medium 2 is an optimized lowserum (5% V/V) medium with epinephrine. The combination of low serum and
selected growth factors ensures successful
growth in vitro and makes this medium
the first choice for renal epithelial cells.

The media are optimized for primary human cells but can also be used for porcine renal epithelial cells.
Related products
Fetal Calf Serum (see page 87)

Renal Epithelial Cell Growth Medium

Renal Epithelial Cell Growth Medium 2

0.05 ml / ml

10 ng / ml

10 ng / ml

5 g / ml

5 g / ml

0.5 g / ml

36 ng / ml

36 ng / ml

Transferrin, holo (human)

5 g / ml

5 g / ml

Triiodo-L-thyronine

4 pg / ml

4 pg / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Renal Epithelial Cell Growth Medium


(Ready-to-use)

500 ml

C-26001

Renal Epithelial Cell Growth Medium Kit

500 ml

C-26011

Renal Epithelial Cell Basal Medium

500 ml

C-26021

Renal Epithelial Cell Basal Medium,


phenol red-free

500 ml

C-26022

Renal Epithelial Cell Growth Medium


SupplementMix

for 500 ml

C-39604

Renal Epithelial Cell Growth Medium


SupplementPack

for 500 ml

C-39600

Renal Epithelial Cell Growth Medium 2


(Ready-to-use)

500 ml

C-26030

Renal Epithelial Cell Growth Medium 2 Kit

500 ml

C-26130

Renal Epithelial Cell Basal Medium 2

500 ml

C-26230

Renal Epithelial Cell Basal Medium 2,


phenol red-free

500 ml

C-26235

Renal Epithelial Cell Growth Medium 2


SupplementMix

for 500 ml

C-39606

Renal Epithelial Cell Growth Medium 2


SupplementPack

for 500 ml

C-39605

3 Media and Sera

69

Low Serum

Recommended for
Human

Placental Epithelial Cells


(see page 26)

The PromoCell Placental Epithelial Cell


Growth Medium is a low-serum (5%
V/V) medium optimized for the cultivation of epithelial cells from the amniotic
membrane of the placenta. The combination of low serum und selected growth
factors ensures successful growth in vitro.

Related products
Fetal Calf Serum (see page 87)

Media and Sera

Placental Epithelial Cell Growth


Medium

Final supplement concentrations


(after addition to the medium)
Fetal Calf Serum
Epidermal Growth Factor
(recombinant human)
Insulin (recombinant human)

0.05 ml / ml
10 ng / ml
5 g / ml

Epinephrine

0.5 g / ml

Hydrocortisone

36 ng / ml

Transferrin, holo (human)

5 g / ml

Triiodo-L-thyronine

4 pg / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Placental Epithelial Cell Growth Medium


(Ready-to-use)

500 ml

C-26040

Placental Epithelial Cell Growth


Medium Kit

500 ml

C-26140

Placental Epithelial Cell Basal Medium

500 ml

C-26240

Placental Epithelial Cell Basal Medium,


phenol red-free

500 ml

C-26245

Placental Epithelial Cell Growth Medium


SupplementMix

for 500 ml

C-39612

Placental Epithelial Cell Growth Medium


SupplementPack

for 500 ml

C-39610

70

3 Media and Sera

Serum-Free
3.1.6 Fibroblast Media
Fibroblast Growth Medium
Recommended for

The PromoCell Fibroblast Growth Medium is a serum-free medium optimized


for the cultivation of neonatal and juvenile fibroblasts.

The medium is optimized for primary human cells but can also be used for rat,
porcine, and bovine fibroblasts as well as
fibroblast cell lines.

Human Dermal Fibroblasts,


juvenile foreskin (see page 27)

Media and Sera

Normal

Final supplement concentrations


(after addition to the medium)
Basic Fibroblast Growth Factor
(recombinant human)

1 ng / ml

Insulin (recombinant human)

5 g / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Fibroblast Growth Medium (Ready-to-use)

500 ml

C-23010

Fibroblast Growth Medium Kit

500 ml

C-23110

Fibroblast Basal Medium

500 ml

C-23210

Fibroblast Basal Medium, phenol red-free

500 ml

C-23215

Fibroblast Growth Medium


SupplementMix

for 500 ml

C-39315

Fibroblast Growth Medium


SupplementPack

for 500 ml

C-39310

Low Serum
Fibroblast Growth Medium 2
Recommended for
Normal

Human Dermal Fibroblasts,


adult donor (see page 27)
Human Pulmonary Fibroblasts
(see page 28)
Human Aortic Adventitial Fibroblasts
(see page 28)
Human Uterine Fibroblasts
(see page 29)
Human Villious Mesenchymal
Fibroblasts (see page 29)

PromoCell Fibroblast Growth Medium 2


is a low-serum (2% V/V) medium developed for adult human fibroblasts.
The medium is optimized for primary human cells but can also be used for rat,
porcine, and bovine fibroblasts as well as
fibroblast cell lines.

Final supplement concentrations


(after addition to the medium)
Fetal Calf Serum

0.02 ml / ml

Basic Fibroblast Growth Factor


(recombinant human)

1 ng / ml

Insulin (recombinant human)

5 g / ml

Related products
Calf Serum (see page 87)

Fetal

3 Media and Sera

71

Product

Size

Catalog Number

Fibroblast Growth Medium 2


(Ready-to-use)

500 ml

C-23020

Fibroblast Growth Medium 2 Kit

500 ml

C-23120

Fibroblast Basal Medium 2

500 ml

C-23220

Fibroblast Basal Medium 2,


phenol red-free

500 ml

C-23225

Fibroblast Growth Medium 2


SupplementMix

for 500 ml

C-39325

Fibroblast Growth Medium 2


SupplementPack

for 500 ml

C-39320

Fibroblast Growth Medium 3


Recommended for
Human

Cardiac Fibroblasts
(see page 28)

PromoCell Fibroblast Growth Medium 3


has been developed for the in vitro cultivation of adult Human Cardiac Fibroblasts (HCF). The medium is optimized
for primary human cells but can also be
used for porcine cardiac fibroblasts.

Related products:
calf serum (see page 87)

Fetal

Final supplement concentrations


(after addition to the medium)
Fetal Calf Serum

0.1 ml / ml

Basic Fibroblast Growth Factor


(recombinant human)

1 ng / ml

Insulin (recombinant human)

5 g / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Fibroblast Growth Medium 3


(Ready-to-use)

500 ml

C-23025

Fibroblast Growth Medium 3 Kit

500 ml

C-23130

Fibroblast Basal Medium 3

500 ml

C-23230

Fibroblast Basal Medium 3,


phenol red-free

500 ml

C-23235

Fibroblast Growth Medium 3


SupplementMix

for 500 ml

C-39345

Fibroblast Growth Medium 3


SupplementPack

for 500 ml

C-39350

Media and Sera

https://www.promocell.com/shop

72

3 Media and Sera

Low Serum
3.1.7 Follicle Dermal Papilla Cell Medium
Follicle Dermal Papilla Cell Growth
Medium
Recommended for
Follicle Dermal Papilla Cells
(see page 30)

Media and Sera

Human

The PromoCell Follicle Dermal Papilla


Cell Growth Medium is a low-serum
(4% V/V) medium. The combination of
low serum and growth factors leads to
excellent proliferation of human follicle
dermal papilla cells in vitro.

Related products
Fetal Calf Serum (see page 87)

Final supplement concentrations


(after addition to the medium)
Fetal Calf Serum
Bovine Pituitary Extract

0.04 ml / ml
0.004 ml / ml

Basic Fibroblast Growth Factor


(recombinant human)

1 ng / ml

Insulin (recombinant human)

5 g / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Follicle Dermal Papilla Cell Growth


Medium (Ready-to-use)

500 ml

C-26501

Follicle Dermal Papilla Cell Growth


Medium Kit

500 ml

C-26502

Follicle Dermal Papilla Cell Basal Medium

500 ml

C-26500

Follicle Dermal Papilla Cell Basal Medium,


phenol red-free

500 ml

C-26505

Follicle Dermal Papilla Cell Growth


Medium SupplementMix

for 500 ml

C-39625

Follicle Dermal Papilla Cell Growth


Medium SupplementPack

for 500 ml

C-39620

3 Media and Sera

73

Serum-Free

Hepatocyte Growth Medium


Hepatocyte Maintenance Medium
Recommended for
Human

Hepatocytes (see page 31)

The PromoCell Hepatocyte Growth Medium is a serum-free medium developed


for the cultivation of human hepato-

Final supplement concentrations


(after addition to the medium)
Epidermal Growth Factor
(recombinant human)
Insulin (recombinant human)

cytes. It is supplemented with well chosen growth factors and hormones and
suited for long-term cultivation.

The media are optimized for primary human hepatocytes but can also be used
for porcine hepatocytes.

In situations where the media compounds


of the Hepatocyte Growth Medium interfere with certain experimental setups, we
recommend using the PromoCell Hepatocyte Maintenance Medium. This medium
is developed for short-term cultivation
and contains fewer supplements.

Note: For culturing Hepatocytes,


Collagen type I coated culture vessels
are necessary.
Under standard in vitro cell culture
conditions, mature human hepatocytes
usually do not survive for longer than
10-14 days and do not proliferate.

Hepatocyte Growth Medium

Hepatocyte Maintenance Medium

10 ng / ml

5 g / ml

6 ng / ml

Hydrocortisone

0.5 g / ml

Transferrin, holo (human)

10 g / ml

Ascorbic Acid
Bovine Serum Albumin-Fatty Acid Free
(BSA-FAF)
Dexamethasone

250 g / ml

3.75 mg / ml

400 ng / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Hepatocyte Growth Medium


(Ready-to-use)

500 ml

C-25010

Hepatocyte Growth Medium Kit

500 ml

C-25110

Hepatocyte Basal Medium

500 ml

C-25210

Hepatocyte Basal Medium,


phenol red-free

500 ml

C-25215

Hepatocyte Growth Medium


SupplementMix

for 500 ml

C-39642

Hepatocyte Growth Medium


SupplementPack

for 500 ml

C-39640

Hepatocyte Maintenance Medium


(Ready-to-use)

500 ml

C-25020

Hepatocyte Maintenance Medium Kit

500 ml

C-25120

Hepatocyte Maintenance Medium


SupplementMix

for 500 ml

C-39652

Hepatocyte Maintenance Medium


SupplementPack

for 500 ml

C-39650

Media and Sera

3.1.8 Hepatocyte Media

74

3 Media and Sera

Serum-Free
3.1.9 Keratinocyte Medium
Keratinocyte Growth Medium 2
Recommended for

Media and Sera

Normal

Human Epidermal
Keratinocytes, juvenile foreskin,
single donor (see page 32)
Normal Human Epidermal
Keratinocytes, juvenile foreskin,
pooled (see page 32)

Normal

Human Epidermal
Keratinocytes, adult, single donor
(see page 32)
Normal Human Epidermal

Keratinocytes, adult, pooled
(see page 32)
PromoCell Keratinocyte Growth Medium 2 is a serum-free medium developed
for the cultivation of epidermal keratino-

cytes without feeder cells.


The medium is optimized for primary human cells but can also be used for horse
and porcine keratinocytes, as well as
mouse and rat keratinocytes after adapting the CaCl2 concentration. In addition,
it is suited for rat corneal keratinocytes.
Related products
-Solution (see page 98)
2

CaCl

Final supplement concentrations


(after addition to the medium)
Bovine Pituitary Extract

0.004 ml / ml

Epidermal Growth Factor


(recombinant human)

0.125 ng / ml

Insulin (recombinant human)

5 g / ml

Hydrocortisone

0.33 g / ml

Epinephrine

0.39 g / ml

Transferrin, holo (human)


CaCl2

10 g / ml
0.06 mM

https://www.promocell.com/shop
Product

Size

Catalog Number

Keratinocyte Growth Medium 2


(Ready-to-use)

500 ml

C-20011

Keratinocyte Growth Medium 2 Kit

500 ml

C-20111

Keratinocyte Basal Medium 2

500 ml

C-20211

Keratinocyte Basal Medium 2,


phenol red-free

500 ml

C-20216

Keratinocyte Growth Medium 2


SupplementMix

for 500 ml

C-39016

Keratinocyte Growth Medium 2


SupplementPack

for 500 ml

C-39011

3 Media and Sera

75

Serum-Free
3.1.10 Melanocyte Media

Recommended for
Normal

Human Epidermal Melanocytes,


juvenile foreskin (see page 33)

The PromoCell Melanocyte Growth


Medium is a serum-free, PMA (Phorbol
Myristate Acetate) containing medium
optimized for the cultivation of human
melanocytes. Since PMA is a tumor promoting mitogen, it may interfere with

some experimental approaches. In these


cases, we recommend using Melanocyte
Growth Medium M2.
The medium is optimized for primary human cells but can also be used for horse,
porcine, murine, and rat melanocytes.
Media and Sera

Melanocyte Growth Medium

Final supplement concentrations


(after addition to the medium)
Bovine Pituitary Extract

0.004 ml / ml

Basic Fibroblast Growth Factor


(recombinant human)

1 ng / ml

Insulin (recombinant human)

5 g / ml

Hydrocortisone

0.5 g / ml

Phorbol Myristate Acetate

10 ng / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Melanocyte Growth Medium (Ready-to-use)

500 ml

C-24010

Melanocyte Growth Medium Kit

500 ml

C-24110

Melanocyte Basal Medium

500 ml

C-24210

Melanocyte Basal Medium, phenol red-free

500 ml

C-24215

Melanocyte Growth Medium SupplementMix

for 500 ml

C-39415

Melanocyte Growth Medium SupplementPack

for 500 ml

C-39410

Serum-Free
Melanocyte Growth Medium M2
Recommended for
Normal

Human Epidermal
Melanocytes, juvenile foreskin,
cultured in M2 Medium (see page 33)

Normal

Human Epidermal
Melanocytes, adult donor, cultured
in M2 Medium (see page 33)
The unique PromoCell Melanocyte
Growth Medium 2 is a serum-free and
PMA (Phorbol Myristate Acetate) free

medium optimized for the cultivation of


human melanocytes.
It is optimized for primary human cells
but can also be used for equine, porcine,
murine, and rat melanocytes. It is also
suitable for human pigment epithelial
cells, neuronal cells and melanoma cells.

List of supplements and their concentrations: confidential


https://www.promocell.com/shop
Product

Size

Catalog Number

Melanocyte Growth Medium M2


(Ready-to-use)

500 ml

C-24300

Melanocyte Growth Medium M2


(Ready-to-use), phenol red-free

500 ml

C-24305

Melanocyte Basal Medium M2

500 ml

C-24400

Melanocyte Basal Medium M2,


phenol red-free

500 ml

C-24405

for 500 ml

C-39420

Melanocyte Growth Medium M2


SupplementMix

76

3 Media and Sera

3.1.11 Osteoblast Media


Osteoblast Growth Medium
Recommended for

Media and Sera

Human

Osteoblasts (see page 34)

PromoCell Osteoblast Growth Medium


was developed to establish and maintain human osteoblast cultures. The
fetal calf serum in the medium is strictly
pretested for optimal performance.

The media are optimized for primary human cells but can also be used for bovine, murine, and rat osteoblasts as well
as osteoblast cell lines.

Final supplement concentration


(after addition to the medium)
Fetal Calf Serum

0.1 ml / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Osteoblast Growth Medium


(Ready-to-use)

500 ml

C-27001

Osteoblast Basal Medium

500 ml

C-27010

Osteoblast Basal Medium,


phenol red-free

500 ml

C-27015

for 500 ml

C-39615

Osteoblast Growth Medium


SupplementMix

Osteoblast Mineralization Medium


Recommended for
Human

Osteoblasts (see page 34)

PromoCell Osteoblast Mineralization


Medium was developed for the efficient induction of strong mineralization
in human osteoblast cultures. Extensive
extracellular calcium deposits can be ob-

served after 21 days, following an easy


one-step protocol (see page 34, Fig. 3).

List of supplements and their concentrations: confidential


https://www.promocell.com/shop
Product
Osteoblast Mineralization Medium

Size

Catalog Number

100 ml

C-27020

3 Media and Sera

77

Low Serum/Serum-Free
3.1.12 Preadipocyte/Adipocyte Media

Preadipocyte Differentiation
Medium
Adipocyte Nutrition Medium
Recommended for
Human

White Preadipocytes
(see page 35)

The PromoCell Preadipocyte Growth


Medium is a low-serum (5% V/V) medium optimized for the expansion of human preadipocytes.
The Preadipocyte Differentiation Medium is a serum-free medium, which
induces the differentiation of preadipocytes into mature adipocytes.

The media are optimized for primary human cells but can also be used for murine
preadipocytes/adipocytes.
Related products
Calf Serum (see page 87)

Fetal

Media and Sera

Preadipocyte Growth Medium

The mature adipocytes can be cultivated


using the Adipocyte Nutrition Medium.

Final supplement concentrations


(after addition to the medium)
Fetal Calf Serum
Endothelial Cell Growth Supplement
Epidermal Growth Factor
(recombinant human)
Hydrocortisone

Preadipocyte
Growth Medium

Preadipocyte
Differentiation Medium

Adipocyte
Nutrition Medium

0.05 ml / ml

0.03 ml / ml

0.004 ml / ml

10 ng / ml

1 g / ml

d-Biotin

8 g / ml

8 g / ml

Insulin (recombinant human)

0.5 g / ml

0.5 g / ml

Dexamethasone

400 ng / ml

400 ng / ml

IBMX

44 g / ml

L-Thyroxine

9 ng / ml

Ciglitazone

3 g / ml

90 g / ml

Heparin

https://www.promocell.com/shop
Product

Size

Catalog Number

Preadipocyte Growth Medium (Ready-to-use)

500 ml

C-27410

Preadipocyte Growth Medium Kit

500 ml

C-27417

Preadipocyte Basal Medium

500 ml

C-27411

Preadipocyte Basal Medium,


phenol red-free

500 ml

C-27415

Preadipocyte Growth Medium


SupplementMix

for 500 ml

C-39425

Preadipocyte Growth Medium


SupplementPack

for 500 ml

C-39427

Preadipocyte Differentiation Medium


(Ready-to-use)

500 ml

C-27436

Preadipocyte Differentiation Medium Kit

500 ml

C-27437

Preadipocyte Differentiation Medium


SupplementMix

for 500 ml

C-39436

Preadipocyte Differentiation Medium


SupplementPack

for 500 ml

C-39437

Adipocyte Nutrition Medium (Ready-to-use)

500 ml

C-27438

Adipocyte Nutrition Medium Kit

500 ml

C-27439

Adipocyte Basal Medium

500 ml

C-27431

Adipocyte Basal Medium, phenol red-free

500 ml

C-27435

Adipocyte Nutrition Medium SupplementMix

for 500 ml

C-39438

Adipocyte Nutrition Medium SupplementPack

for 500 ml

C-39439

78

3 Media and Sera

Low Serum/Serum-Free
3.1.13 Skeletal Muscle Cell Media
Skeletal Muscle Cell Growth
Medium
Skeletal Muscle Cell Differentiation
Medium

Media and Sera

Recommended for
Human

Skeletal Muscle Cells


(see page 36)

The PromoCell Skeletal Muscle Cell


Growth Medium is a low-serum (5%
V/V) medium optimized for the expansion of human skeletal muscle cells.

The media are optimized for primary human cells but can also be used for rat,
murine, and rabbit skeletal muscle cells.

The unique Skeletal Muscle Cell Differentiation Medium is a serum-free


medium that induces the fusion of
myoblasts to myotubes with typical
multinucleated syncytia.

Fetal

Related products
Calf Serum (see page 87)

For more information please see


www.promocell.com/knowledge-base

Final supplement concentrations


(after addition to the medium)

Skeletal Muscle Cell


Growth Medium

Skeletal Muscle Cell


Differentiation Medium

Fetal Calf Serum

0.05 ml / ml

Fetuin (bovine)

50 g / ml

Epidermal Growth Factor


(recombinant human)

10 ng / ml

Basic Fibroblast Growth Factor


(recombinant human)

1 ng / ml

Insulin (recombinant human)

10 g / ml

10 g / ml

Dexamethasone

0.4 g / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Skeletal Muscle Cell Growth Medium


(Ready-to-use)

500 ml

C-23060

Skeletal Muscle Cell Growth Medium Kit

500 ml

C-23160

Skeletal Muscle Cell Basal Medium

500 ml

C-23260

Skeletal Muscle Cell Basal Medium,


phenol red-free

500 ml

C-23265

Skeletal Muscle Cell Growth Medium


SupplementMix

for 500 ml

C-39365

Skeletal Muscle Cell Growth Medium


SupplementPack

for 500 ml

C-39360

Skeletal Muscle Cell Differentiation


Medium (Ready-to-use)

500 ml

C-23061

Skeletal Muscle Cell Differentiation


Medium SupplementMix

for 500 ml

C-39366

3 Media and Sera

79

Low Serum

Smooth Muscle Cell Growth


Medium 2
Recommended for
Human

Umbilical Artery Smooth


Muscle Cells (see page 39)
Human Coronary Artery Smooth
Muscle Cells (see page 38)
Human Pulmonary Artery Smooth
Muscle Cells (see page 38)

Human

Aortic Smooth Muscle Cells


(see page 37)
Human Bronchial Smooth Muscle
Cells (see page 40)
Human Tracheal Smooth Muscle Cells
(see page 39)
Human Bladder Smooth Muscle Cells
(see page 40)
Human Prostate Smooth Muscle Cells
(see page 41)
Human Uterine Smooth Muscle Cells
(see page 41)

The PromoCell Smooth Muscle Cell


Growth Medium 2 is a low-serum (5%
V/V) medium developed to establish and
maintain smooth muscle cell cultures.
The medium is optimized for primary
human cells but can also be used for
bovine, porcine, murine, dog, and rat
smooth muscle cells.
Related products
Fetal Calf Serum (see page 87)

Final supplement concentrations


(after addition to the medium)
Fetal Calf Serum
Epidermal Growth Factor
(recombinant human)

0.05 ml / ml
0.5 ng / ml

Basic Fibroblast Growth Factor


(recombinant human)

2 ng / ml

Insulin (recombinant human)

5 g / ml

https://www.promocell.com/shop
Product

Size

Catalog Number

Smooth Muscle Cell Growth Medium 2


(Ready-to-use)

500 ml

C-22062

Smooth Muscle Cell Growth Medium 2 Kit

500 ml

C-22162

Smooth Muscle Cell Basal Medium 2

500 ml

C-22262

Smooth Muscle Cell Basal Medium 2,


phenol red-free

500 ml

C-22267

Smooth Muscle Cell Growth Medium 2


SupplementMix

for 500 ml

C-39267

Smooth Muscle Cell Growth Medium 2


SupplementPack

for 500 ml

C-39262

3.1.15 Freezing Medium


Recommended for
Human

and Animal Primary Cells


Cell Lines
Induced Pluripotent Stem Cells
Embryonic Stem Cells
Established

Serum-Free
The PromoCell Cryo-SFM is an animal
component-free, chemically defined medium with DMSO, methylcellulose, and
other cryoprotective components developed for the cryopreservation of human
and animal primary cells. It ensures high

cell viability, excellent growth rates, and


good attachment of the cells after thawing. The medium is the first choice for all
types of primary cells and cell lines.

https://www.promocell.com/shop
Product
Cryo-SFM

Size

Catalog Number

30 ml
125 ml

C-29910
C-29912

Media and Sera

3.1.14 Smooth Muscle Cell Medium

80

3 Media and Sera

Media and Sera

3.2. Media for Stem and Blood Cells


The PromoCell media for stem and blood
cells are formulated for either the optimal growth of specific cell types or to
support the differentiation into the respective mature cells.
Each lot is tested for its ability to support
growth or differentiation, respectively. In
addition, biochemical and sterility tests
are performed*.
The Media (Ready-to-use) consist of a
100 ml, 250 ml, or 500 ml bottle of Basal
Medium and a vial of SupplementMix.
Adding the SupplementMix to the Basal
Medium results in the complete medium.

Some Media are provided with additional Cytokine Mixes to achieve optimal
performance.
The shelf life of the Basal Medium is
9 months (at 4 to 8C), and that of the
SupplementMix is 12 months (at -20C)
from the date of manufacture. After
adding the SupplementMix, the shelf life
of the complete medium is 6 weeks (at
4 to 8C).
Note: The media for stem and blood cells
do not contain antibiotics.

If you require special media modifications we offer a Custom Media Service starting at 10 bottles per order.
Please ask for details.

3.2.1 Mesenchymal Stem Cell Media


Mesenchymal Stem Cell
Growth Medium
Mesenchymal Stem Cell
Growth Medium DXF
Mesenchymal Stem Cell
Differentiation Media
Recommended for
Human

Mesenchymal Stem Cells from


Bone Marrow, Umbilical Cord Matrix,
and Adipose Tissue (see page 49)

The PromoCell Mesenchymal Stem Cell


Growth Medium supports the expansion of multipotent human Mesenchymal Stem Cells (MSC) without inducing
early senescence and differentiation as
observed in standard culture media.
The Mesenchymal Stem Cell Growth Medium DXF allows for the robust proliferation of multipotent MSC in a chemically
defined and xeno-free culture environment. It is devoid of all animal-derived
components and substances of human
origin with Human Serum Albumin as
the only exception. Fibronectin-coated

plates are necessary in conjunction with


this medium.
In addition, PromoCell offers five
media to efficiently induce differentiation
of MSC into adipogenic, chondrogenic,
osteogenic or neurogenic lineages, respectively (see also Fig. 3, page 50).
Related products
Cell Dissociation Solution ACF
(see page 96)
Fibronectin Solution, human
(see page 99)

List of supplements and their concentrations: confidential


https://www.promocell.com/shop
Product

Size

Catalog Number

Mesenchymal Stem Cell Growth Medium


(Ready-to-use)

500 ml

C-28010

Mesenchymal Stem Cell Growth Medium


DXF (Ready-to-use)

500 ml

C-28019

Fibronectin Solution, human (1 mg/ml)

5 ml

C-43060

Mesenchymal Stem Cell Adipogenic


Differentiation Medium (Ready-to-use)

100 ml

C-28011

Mesenchymal Stem Cell Chondrogenic


Differentiation Medium (Ready-to-use)

100 ml

C-28012

Mesenchymal Stem Cell Chondrogenic


Differentiation Medium w/o Inducers
(Ready-to-use)

100 ml

C-28014

Mesenchymal Stem Cell Osteogenic


Differentiation Medium (Ready-to-use)

100 ml

C-28013

Mesenchymal Stem Cell Neurogenic


Differentiation Medium (Ready-to-use)

100 ml

C-28015

*Certificates of analysis are available on request.

3 Media and Sera

81

3.2.2 Pericyte Medium


Pericyte Growth Medium
Recommended for
Human

Pericytes (see page 51)

PromoCell Pericyte Growth Medium has


been developed and optimized for the in
vitro cultivation of human pericytes from
all tissues.
Whereas in standard culture media peri-

cytes tend to grow slowly and induce


early senescence, PromoCell Pericyte
Growth Medium optimally supports cell
proliferation and maintenance of multipotency in these cells.

List of supplements and their concentrations: confidential

Product
Pericyte Growth Medium (Ready-to-use)

Size

Catalog Number

500 ml

C-28040

3.2.3 Hematopoietic Progenitor Cell Media


Hematopoietic Progenitor Cell
Expansion Medium DXF
Hematopoietic Progenitor Medium
Recommended for
Human

Mononuclear Cells from


Cord Blood (see page 57)
Human CD34+ Progenitor Cells
(see page 52)
Human CD133+ Progenitor Cells
(see page 53)
Human Hematopoietic Progenitor
Cells
The PromoCell Hematopoietic Progenitor
Cell (HPC) Expansion Medium DXF has
been designed for the maximum expan-

sion of primitive HPCs in a fully defined,


xeno-free culture environment. Due to
the utilization of exclusively synthetic,
recombinant or plant-sourced materials,
this medium is chemically defined and
free of all animal-derived components
and substances of human origin with
Human Serum Albumin as the only
exception.
Note: The medium must be supplemented with additional cytokines, e.g.
Cytokine Mix E (see below). Individual
cytokines can be found on our website
www.promokine.info or page 179.
Cytokine Mix E is a ready-to-use mixture
of growth factors for the HPC Expansion
Medium DXF. The animal component-free,

defined formula contains recombinant human TPO, SCF, flt-3 ligand and IL-3. The
optimized formulation allows vigorous
expansion of hematopoietic progenitor
cells, while maintaining their primitive
phenotype. Differentiation is largely
inhibited.
The PromoCell Hematopoietic Progenitor Medium has been developed
for the expansion of hematopoietic
progenitors by means of differentiation
into more committed lineages of blood
cells. Hence, this progenitor medium
is suitable for CFU-based assays, e.g.
long-term culture initiating cell (LTC-IC)
assays.

For more information please see


www.promocell.com/knowledge-base

List of supplements and their concentrations: confidential


https://www.promocell.com/shop
Product
Hematopoietic Progenitor Cell
Expansion Medium DXF
Cytokine Mix E
for HPC Expansion Medium DXF
Hematopoietic Progenitor Medium
(Ready-to-use)

Size

Catalog Number

500 ml

C-28021

1 ml (sufficient for 100 ml Medium)


5 ml (sufficient for 500 ml Medium)

C-39890
C-39891

100 ml

C-28020

Media and Sera

https://www.promocell.com/shop

82

3 Media and Sera

3.2.4 Endothelial Progenitor Medium


Endothelial Progenitor Medium
Recommended for
Mononuclear Cells
(see page 56)
Human CD34+ Progenitor Cells
(see page 52)
Human Endothelial Progenitor Cells
Media and Sera

Human

The PromoCell Endothelial Progenitor


Medium has been developed for the
expansion of endothelial progenitors by
means of differentiation into more committed lineages of endothelial cells.
Hence, this progenitor medium is suitable for the differentiation of primitive
progenitors into mature endothelial cells
as well as for CFU-based assays, e.g. the

Hill-Assay for endothelial progenitor cells (Hill et al. 2003, N Engl J Med
348: 593-600), which is carried out on
fibronectin-coated plates.

List of supplements and their concentrations: confidential


https://www.promocell.com/shop
Product
Endothelial Progenitor Medium
(Ready-to-use)

Size

Catalog Number

100 ml

C-28025

3.2.5 Dendritic Cell Generation Media


DC Generation Medium
DC Generation Medium DXF
Monocyte Attachment Medium
Recommended for
Human

Monocytes, freshly isolated


and cryopreserved (see page 54)
Human Mononuclear Cells, freshly
isolated
The PromoCell Dendritic Cell (DC) Generation Media have been developed for the
easy and efficient generation of immature
as well as fully mature myeloid Dendritic
Cells from peripheral blood monocytes.

For more information please see


www.promocell.com/knowledge-base

PromoCell DC Generation Medium allows for the generation of DC from


cryopreserved as well as freshly isolated
cells. In the latter case, the Monocyte Attachment Medium is also needed.
The DC Generation Medium DXF is a
chemically defined and xeno-free formulation for use with freshly isolated
cells. It is free of all animal derived components and substances of human origin
with Human Serum Albumin as the only
exception.
The DC Base Media do not include cytokines and must therefore be supplemented according to the users individual
needs.

The Monocyte Attachment Medium


allows for the efficient adherence selection of Monocytes from freshly isolated Mononuclear Cells (MNC) while
maintaining optimal cell health. Thus,
the time-consuming and costly immunomagnetic purification of monocytes
prior to dendritic cell generation is not
necessary.
Note: See our Instruction Manual and
Application Note for details. Please visit
www.promocell.com/application-notes.

3 Media and Sera

83

List of supplements and their concentrations: confidential

Product

Size

Catalog Number

DC Generation Medium (Ready-to-use)

250 ml

C-28050

DC Generation Medium DXF


(Ready-to-use)

250 ml

C-28052

DC Base Medium

250 ml

C-28053

DC Base Medium DXF

250 ml

C-28054

Monocyte Attachment Medium


(Ready-to-use)

250 ml

C-28051

3.2.6 Macrophage Generation Media


M1-Macrophage Generation
Medium DXF
M2-Macrophage Generation
Medium DXF
Monocyte Attachment Medium
Recommended for
Human

Mononuclear Cells, freshly


isolated
Human Monocytes, freshly isolated
The PromoCell Macrophage Generation Media were developed for
easy and efficient generation of
M1- or M2-polarized macrophages from
peripheral blood monocytes. A detailed
step-by-step protocol is available also,
which allows optional user-customized
macrophage activation.

Completely defined

In combination with the Monocyte


Attachment Medium, freshly isolated
peripheral blood mononuclear cells can
be directly used as a starting material.
The PromoCell M1-Macrophage Generation Medium DXF allows for the
generation of M1-polarized macrophages from freshly isolated cells, whereas the M2-Macrophage Generation
Medium DXF produces M2-polarized
macrophages. The chemically defined
and xeno-free media formulations are
free of all animal derived components
and substances of human origin with
Human Serum Albumin as the only
exception.

The Monocyte Attachment Medium allows for the efficient adherence selection
of Monocytes from freshly isolated
Mononuclear Cells (MNC) while maintaining optimal cell health. Thus, the
time-consuming and costly immunomagnetic purification of monocytes prior
to macrophage differentiation is not
necessary.
Note: See our Instruction Manual and
Application Note for details. Please visit
www.promocell.com/application-notes.
Related products
Detachment Solution
DXF (see page 96)

Macrophage

The Macrophage Base Medium does not


include cytokines and must therefore be
supplemented according to the users
individual needs.

List of supplements and their concentrations: confidential


https://www.promocell.com/shop
Product

Size

Catalog Number

M1-Macrophage Generation Medium DXF


(Ready-to-use)

250 ml

C-28055

M2-Macrophage Generation Medium DXF


(Ready-to-use)

250 ml

C-28056

Macrophage Base Medium DXF

250 ml

C-28057

Monocyte Attachment Medium


(Ready-to-use)

250 ml

C-28051

Media and Sera

https://www.promocell.com/shop

84

3 Media and Sera

3.2.7 Mononuclear Cell Medium


Mononuclear Cell Medium

Human

Recommended for

The PromoCell Mononuclear Cell Medium is a very rich short-term maintenance medium which provides utmost
cell viability.

Mononuclear Cells
from Cord Blood (see page 57)
Human Mononuclear Cells
from Peripheral Blood (see page 56)
Media and Sera

Human

Monocytes (see page 54)

48 hours before proceeding with your


experiments.
Related products
Lymphocyte Separation Medium
1077 (see below)

It is suitable to maintain MNCs for up to

List of supplements and their concentrations: confidential


https://www.promocell.com/shop
Product
Mononuclear Cell Medium (Ready-to-use)

Size

Catalog Number

500 ml

C-28030

3.2.8 Blood Cell Separation Medium


Lymphocyte Separation Medium
1077
Recommended for
Peripheral

blood
cord blood
Buffy coats
Apheresis products
Bone marrow
Umbilical

The PromoCell Lymphocyte Separation


Medium 1077 is a sterile, ready-to-use
solution optimized for the separation of
lymphocytes from whole blood or buffy
coats without any loss of viability. It is
based on Ficoll 400 and sodium diatrizoate and provides optimal physiological
parameters. It has a density of 1,077 g/ml
at +20C.

https://www.promocell.com/shop
Product
Lymphocyte Separation Medium 1077

Size

Catalog Number

500 ml

C-44010

3 Media and Sera

85

3.3 Classical Media


n DMEM Media
Size

Catalog Number

DMEM, w/o L-Glutamine

500 ml

C-71110

DMEM, with L-Glutamine

500 ml

C-71111

DMEM, with 25 mM HEPES,


w/o L-Glutamine

500 ml

C-71115

DMEM, with 25 mM HEPES,


with L-Glutamine

500 ml

C-71116

DMEM, with stable Glutamine

500 ml

C-71120

DMEM, High Glucose (4.5 g/l),


w/o L-Glutamine

500 ml

C-71210

DMEM, High Glucose (4.5 g/l),


with L-Glutamine

500 ml

C-71212

DMEM, High Glucose (4.5 g/l),


with stable Glutamine

500 ml

C-71220

DMEM, High Glucose (4.5 g/l),


with stable Glutamine, with Na-Pyruvate

500 ml

C-71221

DMEM, High Glucose (4.5 g/l),


with L-Glutamine, with Na-Pyruvate

500 ml

C-71222

DMEM, High Glucose (4.5 g/l),


w/o L-Glutamine, with Na-Pyruvate

500 ml

C-71223

n DMEM/Hams F-12 Media


Product

https://www.promocell.com/shop
Size

Catalog Number

DMEM/Hams F-12, w/o L-Glutamine

500 ml

C-71310

DMEM/Hams F-12, with L-Glutamine

500 ml

C-71311

DMEM/Hams F-12, with stable Glutamine

500 ml

C-71320

n Hams Media
Product

https://www.promocell.com/shop
Size

Catalog Number

Hams F-10, w/o L-Glutamine

500 ml

C-72010

Hams F-10, with L-Glutamine

500 ml

C-72020

Hams F-12, w/o L-Glutamine

500 ml

C-72110

Hams F-12, with L-Glutamine

500 ml

C-72120

n Leibovitz L-15 Medium


Product
Leibovitz L-15, w/o L-Glutamine

https://www.promocell.com/shop
Size

Catalog Number

500 ml

C-73110

Media and Sera

Product

https://www.promocell.com/shop

86

3 Media and Sera

n McCoys 5A Media

Media and Sera

Product

https://www.promocell.com/shop
Size

Catalog Number

McCoys 5A, w/o L-Glutamine

500 ml

C-73210

McCoys 5A, with L-Glutamine

500 ml

C-73211

McCoys 5A, with stable Glutamine

500 ml

C-73220

McCoys 5A, with stable Glutamine,


phenol red-free

500 ml

C-73227

n MEM Alpha and MEM D-Valine Media


Product

https://www.promocell.com/shop
Size

Catalog Number

MEM Alpha, w/o Nucleosides,


w/o L-Glutamine

500 ml

C-75012

MEM Alpha, w/o Nucleosides,


with L-Glutamine

500 ml

C-75013

MEM Alpha, with Nucleosides,


with L-Glutamine

500 ml

C-75014

MEM D-Valine, w/o L-Glutamine

500 ml

C-75100

n MEM Earle Media


Product

https://www.promocell.com/shop
Size

Catalog Number

MEM Earle, w/o L-Glutamine

500 ml

C-75210

MEM Earle, with L-Glutamine

500 ml

C-75211

MEM Earle, with stable Glutamine

500 ml

C-75220

n RPMI 1640 Media


Product

https://www.promocell.com/shop
Size

Catalog Number

RPMI 1640, w/o L-Glutamine

500 ml

C-76010

RPMI 1640, with L-Glutamine

500 ml

C-76011

RPMI 1640, with 25 mM HEPES,


w/o L-Glutamine

500 ml

C-76015

RPMI 1640, with 25 mM HEPES,


with L-Glutamine

500 ml

C-76016

RPMI 1640, w/o L-Glutamine,


phenol red-free

500 ml

C-76017

RPMI 1640, with stable Glutamine

500 ml

C-76020

3 Media and Sera

87

The medium requirements of insect cells


are significantly different from vertebrate
cell culture. For instance, the optimal pH
range for most insect cells is more acidic

(pH 6.2 to 6.5). Also, the amino acid


concentration needs to be higher than in
vertebrate cell media.

Mitsuhashi/Maramorosh Insect
Medium

Mitsuhashi and Maramorosh Insect


Medium is well-suited for Mosquito cell
culture and must be supplemented with
FCS prior to use.

Recommended for
Mosquito

Cells
https://www.promocell.com/shop

Product
Mitsuhashi/Maramorosh Insect Medium

Schneiders Drosophila Medium


Recommended for
Dipteran

Size

Catalog Number

500 ml

C-77110

Schneiders Drosophila Medium was


originally developed for the culture of
Drosophila cells but can also be used for
the culture of other dipteran cell lines.

Cells
https://www.promocell.com/shop

Product
Schneiders Drosophila Medium,
w/o L-Glutamine

Size

Catalog Number

500 ml

C-77210

3.5 Sera
Fetal Calf Serum
Recommended for
Primary

Human Cells (all types)

The PromoCell Fetal Calf Sera are high


quality, pretested fetal calf serum batches. They are extensively tested for their
ability to support in vitro expansion of
human primary cells.

In addition, histochemical staining is performed to ensure that the cultured primary cells maintain their cell type specific
marker expression.

https://www.promocell.com/shop
Product
Fetal Calf Serum (EU Quality)
primary human cell culture tested

Size

Catalog Number

100 ml
500 ml

C-37355
C-37350

Media and Sera

3.4 Insect Cell Culture Media

88

3 Media and Sera

MSC-Qualified Fetal Calf Serum


Recommended for
Mesenchymal

Stem Cells (all types)

The PromoCell MSC-Qualified Fetal Calf


Sera are high quality, pre-selected fetal
calf serum batches. They are extensively
tested for their ability to support in vitro
expansion of human Mesenchymal Stem

Cells. In addition, histochemical staining


is performed to ensure that the cultured
stem cells maintain the ability to differentiate into diverse lineages.

Media and Sera

Fig. 1: Expansion and differentiation of


Mesenchymal Stem Cells (MSC) using
media with the PromoCell MSC-Qualified Fetal Calf Serum. Left: Undifferentiated hMSC-BM cultured for 4 passages
(phase contrast). Right: The same cells
after 14 days of adipogenic induction
(Oil Red O staining of lipid droplets
with Hematoxylin counterstain).
https://www.promocell.com/shop
Product
MSC-Qualified Fetal Calf Serum

ESC-Qualified Fetal Calf Serum


Recommended for
Pluripotent

Embryonic Stem Cells


Progenitor Cells
Endothelial Progenitor Cells
Hematopoietic

Size

Catalog Number

100 ml
500 ml

C-37386
C-37385

The PromoCell ESC-Qualified Fetal Calf


Sera are high quality, pre-selected fetal
calf serum batches. They are extensively tested for their ability to support
undifferentiated, feeder-free culture of
pluripotent murine embryonic stem cells
in the presence of leukemia inhibitory

factor. The maintenance of pluripotency


is assayed by immunostaining for basal
pluripotency markers. In addition, this
serum is also recommended for the culture of hematopoietic and endothelial
progenitor cells.

Fig. 2: Growth morphology of murine


embryonic stem cells (mESC). Left:
mESC in standard Fetal Calf Serum.
Note the large, flat, differentiated
cells. Right: mESC after 5 passages in
PromoCell ESC-Qualified Fetal Calf
Serum. The cells are small and have a
compact colony morphology typical for
mESC.
https://www.promocell.com/shop
Product
ESC-Qualified Fetal Calf Serum

Size

Catalog Number

100 ml
500 ml

C-37388
C-37387

Cell Pellets
PromoCell Cell Pellets are an easily accessible source of RNA,
DNA, or proteins from a wide range of human primary cell
types without laborious cell cultivation work. This allows you
to focus on your molecular biology experiments and maximize
your valuable research time.

90 Content Chapter 4

Cell Pellets
4.1

Cell Pellets . . . . . . . . . . . . . . . . . . . . . . . . . 91

4 Cell Pellets

91

4.1 Cell Pellets


Indefinitely

stable at -20C
with established nucleic
acid purification protocols

Compatible
Gene

expression analysis
characterization studies
Northern blots
Southern blots
Western blots/2D gel electrophoresis
Cell

Features
Available

from all PromoCell


cell types
1 million cells per pellet
Stored in RNAlater

PromoCell Cell Pellets are stored in


RNAlater - an aqueous protection solution
that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins
and protects them against degradation.
This makes the PromoCell Cell Pellets
very useful for a wide range of research
applications* like gene expression analysis, cell characterization studies, and protein characterization experiments.

Note: RNAlater denatures proteins.


Therefore, proteins will be suitable for
e.g. Western blotting but not for applications that require native proteins.
Each Cell Pellet is made from 1 million
cells and is dissolved in 200 l RNAlater.
The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell
type specific antigens. On request the
cells from the same lot are available for
cultivation. The Cell Pellets can be stored
indefinitely at -20C.
https://www.promocell.com/shop

Product

Product Description

HAoAF Pellet

Pellet from Human Aortic Adventitial Fibroblasts

C-14037

HAoEC Pellet

Pellet from Human Aortic Endothelial Cells

C-14023

HAoSMC Pellet

Pellet from Human Aortic Smooth Muscle Cells

C-14053

HBdMEC Pellet

Pellet from Human Bladder Microvascular Endothelial Cells

C-14026

HBdSMC Pellet

Pellet from Human Bladder Smooth Muscle Cells

C-14057

HBEpC Pellet

Pellet from Human Bronchial Epithelial Cells

HBMEC Pellet

Pellet from Human Brain Microvascular Endothelial Cells

HBSMC Pellet

Pellet from Human Bronchial Smooth Muscle Cells

C-14055

HCAEC Pellet

Pellet from Human Coronary Artery Endothelial Cells

C-14022

HCASMC Pellet

Pellet from Human Coronary Artery Smooth Muscle Cells

C-14052

HCF Pellet

Pellet from Human Cardiac Fibroblasts

C-14036

HCH Pellet

Pellet from Human Chondrocytes

C-14070

HCM Pellet

Pellet from Human Cardiac Myocytes

C-14080

HCMEC Pellet

Pellet from Human Cardiac Microvascular Endothelial Cells

C-14029

HDBEC adult Pellet

Pellet from Human Dermal Blood Endothelial Cells adult donor

C-14019

HDBEC Pellet

Pellet from Human Dermal Blood Endothelial Cells juvenile foreskin

C-14018

HDLEC adult Pellet

Pellet from Human Dermal Lymphatic Endothelial Cells adult donor

C-14021

HDLEC Pellet

Pellet from Human Dermal Lymphatic Endothelial Cells


juvenile foreskin

C-14020

HDMEC adult Pellet

Pellet from Human Dermal Microvascular Endothelial Cells


adult donor

C-14016

HDMEC Pellet

Pellet from Human Dermal Microvascular Endothelial Cells


juvenile foreskin

C-14015

HDMEC pre-screened Pellet

Pellet from Human Dermal Microvascular Endothelial Cells


pre-screened

C-14017

HFDPC Pellet

Pellet from Human Follicle Dermal Papilla Cells

C-14005

HHpC Pellet

Pellet from Human Hepatocytes

C-14085

HMEpC Pellet

Pellet from Human Mammary Epithelial Cells

C-14066

hMNC-CB pooled Pellet

Pellet from Human Mononuclear Cells from Cord Blood


pooled, ultra-pure

C-14097

hMNC-CB single donor Pellet

Pellet from Human Mononuclear Cells from Cord Blood


single donor, ultra-pure

C-14096

hMNC-PB single donor Pellet

Pellet from Human Mononuclear Cells from Peripheral Blood


single donor, ultra-pure

C-14098

hMNC-PB pooled Pellet

Pellet from Human Mononuclear Cells from Peripheral Blood


pooled, ultra-pure

C-14099

hMoCD14+-PB single donor Pellet

Pellet from Human CD14+ Monocytes from Peripheral Blood,


single donor

C-14110

* More information on RNAlater (FAQs, protocols) at www.invitrogen.com/site/us/en/home/brands/Product-Brand/rnalater.html

Catalog Number

C-14063
Limited Availability

C-14032

Cell Pellets

Applications

92

4 Cell Pellets

Cell Pellets

https://www.promocell.com/shop
Product

Product Description

Catalog Number

hMoCD14+-PB single donor


pre-screened Pellet

Pellet from Human CD14+ Monocytes from Peripheral Blood,


single donor pre-screened

C-14112

hMo-PB single donor Pellet

Pellet from Human Monocytes from Peripheral Blood, single donor

C-14111

hMo-PB single donor


pre-screened Pellet

Pellet from Human Monocytes from Peripheral Blood, single


donor pre-screened

C-14113

hMSC-AT Pellet

Pellet from Human Mesenchymal Stem Cells from Adipose Tissue

C-14092

hMSC-BM Pellet

Pellet from Human Mesenchymal Stem Cells from Bone Marrow

C-14090

hMSC-UC Pellet

Pellet from Human Mesenchymal Stem Cells from Umbilical Cord Matrix

C-14091

HNEpC Pellet

Pellet from Human Nasal Epithelial Cells

C-14062

HOB Pellet

Pellet from Human Osteoblasts

C-14071

HPAEC Pellet

Pellet from Human Pulmonary Artery Endothelial Cells

C-14024

HPASMC Pellet

Pellet from Human Pulmonary Artery Smooth Muscle Cells

C-14051

hPC-PL Pellet

Pellet from Human Pericytes from Placenta

C-14095

HPF Pellet

Pellet from Human Pulmonary Fibroblasts

C-14035

HPlEpC Pellet

Pellet from Human Placental Epithelial Cells

C-14069

HPMEC Pellet

Pellet from Human Pulmonary Microvascular Endothelial Cells

C-14027

HPrSMC Pellet

Pellet from Human Prostate Smooth Muscle Cells

C-14058

HRCEpC Pellet

Pellet from Human Renal Cortical Epithelial Cells

C-14068

HREpC Pellet

Pellet from Human Renal Epithelial Cells

C-14067

HSAEpC Pellet

Pellet from Human Small Airway Epithelial Cells

C-14065

HSaVEC Pellet

Pellet from Human Saphenous Vein Endothelial Cells

C-14025

HTEpC Pellet

Pellet from Human Tracheal Epithelial Cells

C-14064

HTSMC Pellet

Pellet from Human Tracheal Smooth Muscle Cells

C-14056

HUAEC Pellet

Pellet from Human Umbilical Artery Endothelial Cells

C-14013

HUASMC Pellet

Pellet from Human Umbilical Artery Smooth Muscle Cells

C-14050

HUF Pellet

Pellet from Human Uterine Fibroblasts

C-14038

HUtMEC Pellet

Pellet from Human Uterine Microvascular Endothelial Cells

C-14028

HUtSMC Pellet

Pellet from Human Uterine Smooth Muscle Cells

C-14059

HUVEC single donor Pellet

Pellet from Human Umbilical Vein Endothelial Cells, single donor

C-14010

HUVEC pooled Pellet

Pellet from Human Umbilical Vein Endothelial Cells, pooled

C-14011

HUVEC Growth Medium 2 single donor


Pellet

Pellet from Human Umbilical Vein Endothelial Cells isolated in


Growth Medium 2, single donor

C-14008

HUVEC Growth Medium 2 pooled


Pellet

Pellet from Human Umbilical Vein Endothelial Cells isolated in


Growth Medium 2, pooled

C-14009

HUVEC pre-screened Pellet

Pellet from Human Umbilical Vein Endothelial Cells pre-screened

C-14012

HVMF Pellet

Pellet from Human Villous Mesenchymal Fibroblasts

C-14034

HWP subcutaneous Pellet

Pellet from Human White Preadipocytes subcutaneous

C-14072

HWP visceral Pellet

Pellet from Human White Preadipocytes visceral

C-14073

NHDF adult Pellet

Pellet from Normal Human Dermal Fibroblasts adult donor

C-14031

NHDF Pellet

Pellet from Normal Human Dermal Fibroblasts juvenile foreskin

C-14030

NHEK adult, single donor Pellet

Pellet from Normal Human Epidermal Keratinocytes adult, single donor

C-14002

NHEK adult pooled Pellet

Pellet from Normal Human Epidermal Keratinocytes adult, pooled

C-14004

NHEK.f single donor Pellet

Pellet from Normal Human Epidermal Keratinocytes


juvenile foreskin, single donor

C-14001

NHEK.f pooled Pellet

Pellet from Normal Human Epidermal Keratinocytes


juvenile foreskin, pooled

C-14003

NHEM M2 adult Pellet

Pellet from Normal Human Epidermal Melanocytes


adult donor, cultured in M2 Medium

C-14043

NHEM.f M2 Pellet

Pellet from Normal Human Epidermal Melanocytes


juvenile foreskin, cultured in M2 Medium

C-14042

NHEM.f Pellet

Pellet from Normal Human Epidermal Melanocytes


juvenile foreskin

C-14040

SkMC Pellet

Pellet from Human Skeletal Muscle Cells

C-14060

Reagents and Supplements


PromoCell offers a broad range of reagents and supplements
for cell culture. This includes enzyme mixtures for tissue and
cell dissociation, buffers for daily cell culture work, antibiotics
and antimycotics in different concentrations, components
to supplement various culture media, such as cytokines and
growth factors, and reagents needed for cell assays. All of these
are intensively tested and meet our stringent quality criteria.

94 Content Chapter 5

Reagents and Supplements


5.1

Detachment Reagents . . . . . . . . . . . . . . . . 95

5.2

Antibiotics and Antimycotics . . . . . . . . . . . . 97

5.3

Buffers and Salt Solutions . . . . . . . . . . . . . . 98

5.4 Supplements . . . . . . . . . . . . . . . . . . . . . . . 99

5 Reagents and Supplements

95

5.1 Detachment Reagents

Recommended for
All

human primary cell types

The PromoCell DetachKit was developed


for gentle and effective detachment of
adherent primary human cells in culture.
Each batch is tested on primary human cells.

The DetachKit is optimized for primary


human cells but can also be used for primary animal cells and cell lines.
The kit contains three components:
HepesBSS (30 mM Hepes)
Trypsin / EDTA Solution
(0.04% / 0.03%)
Trypsin Neutralizing Solution (TNS)

https://www.promocell.com/shop
Product
DetachKit

DetachKit 2
The PromoCell DetachKit 2 was developed for very gentle detachment of primary human cells in culture. It contains
less trypsin and EDTA than the standard
DetachKit and is suited for applications,
which require very gentle cell handling.

Size

Catalog Number

30 ml
125 ml
250 ml

C-41200
C-41210
C-41220

The DetachKit 2 is optimized for primary


human cells but can also be used for primary animal cells and cell lines.
The kit contains three components:
(30 mM Hepes)
Trypsin / EDTA Solution
(0.025% / 0.01%)
Trypsin Neutralizing Solution (TNS)
HepesBSS

https://www.promocell.com/shop
Product
DetachKit 2

Accutase-Solution
Recommended for
Primary

cells
lines
Tissue dissociation
Cell

Size

Catalog Number

30 ml
125 ml
250 ml

C-41202
C-41212
C-41222

Accutase is an alternative cell detachment solution to trypsin and can also


be used for tissue dissociation. It was
developed for very gentle and effective
detachment of adherent cells. The well
balanced combination of proteolytic and
collagenolytic activities ensures that sur-

face proteins and epitopes stay entirely


intact. Accutase is therefore perfectly
suited for applications, which require an
unchanged cell surface.
Each batch of the PromoCell AccutaseSolution is tested on primary human
cells.
https://www.promocell.com/shop

Product
Accutase-Solution,
primary human cell culture tested

Size

Catalog Number

100 ml

C-41310

Reagents and
Supplements

DetachKit

96

5 Reagents and Supplements

Cell Dissociation Solution ACF


Cell Dissociation Solution ACF is a proprietary non-enzymatic reagent for de-

tachment of adherent cell types from


tissue culture plastic or glass surfaces.
Due to its gentle yet effective action it
presents a good animal component-free

alternative to trypsin and other enzymatic


detachment solutions.

https://www.promocell.com/shop
Product

Reagents and
Supplements

Cell Dissociation Solution ACF

Macrophage Detachment Solution DXF


The Macrophage Detachment Solution DXF is a non-enzymatic reagent

Size

Catalog Number

100 ml

C-41320

especially optimized for the gentle


detachment of strongly adherent macrophages.

https://www.promocell.com/shop
Product
Macrophage Detachment Solution DXF

Trypsin Solutions
Trypsin is still the most widely employed
enzyme used for cell detachment.

Size

Catalog Number

250 ml

C-41330

PromoCells Trypsin Solutions exhibit


standardized enzyme activity and contain trypsin from extra-pure lots.

https://www.promocell.com/shop
Product

Size

Catalog Number

Trypsin / EDTA (0.04% / 0.03%)

30 ml
125 ml
250 ml

C-41000
C-41010
C-41020

Trypsin / EDTA 2 (0.025% / 0.01%)

30 ml
125 ml
250 ml

C-41002
C-41012
C-41022

Trypsin-EDTA PBS 1:250 (0.05% / 0.02%),


w/o Ca++/Mg++

100 ml

C-41060

Trypsin-EDTA PBS 1:250, (0.5% / 0.2%),


w/o Ca++/Mg++, 10x conc.

100 ml

C-41070

Trypsin Neutralizing Solution

https://www.promocell.com/shop
Product
Trypsin Neutralizing Solution (TNS)
(0.05% Trypsin Inhibitor in 0.1% BSA)

Size

Catalog Number

30 ml
125 ml
250 ml

C-41100
C-41110
C-41120

5 Reagents and Supplements

97

5.2 Antibiotics and Antimycotics


However, antibiotics may not only harm
contaminating organisms, but can also
unfavorably alter the physiology and
lifespan of primary human cells. Therefore PromoCell does not recommend
the routine use of antibiotics in cell

n Antibiotics and Antimycotics


Product

culture as a general preventive measure.


As a further application antibiotics may
be utilized in genetic selection procedures, e.g. G-418.

https://www.promocell.com/shop
Size

Catalog Number

Pen/Strep Solution
(10,000 IU/ml / 10 mg/ml)

100 ml

C-42010

Pen/Strep/Fungizone Solution
(10,000 IU/ml / 10,000 g/ml / 25 g/ml)

100 ml

C-42020

Amphotericin B Solution (0.25 mg/ml)

100 ml

C-42040

Gentamicin Solution (5 mg/ml)

100 ml

C-42060

n Selection Antibiotics
Product

https://www.promocell.com/shop
Size

Catalog Number

G-418 Sulphate Solution (50 mg/ml)

20 ml

C-42094

Hygromycin B Solution (50 mg/ml)

20 ml

C-42095

Reagents and
Supplements

Antibiotics are used in order to prevent


microbial contaminations in cell culture
during initial isolation steps or to cure
precious cultures exhibiting microbial
contamination, e.g. with mycoplasma or
yeast/fungi.

98

5 Reagents and Supplements

5.3 Buffers and Salt Solutions


Buffers and Salt Solutions are frequently
used in tissue culture, cell isolation procedures, and molecular biology.

For convenience, PromoCell offers


ready-to-use Buffers and Salt Solutions.
They are prepared from raw materials of

n Dulbeccos Phosphate Buffered Saline

Reagents and
Supplements

Product
Dulbeccos PBS

the highest quality using tissue culture


grade water.

https://www.promocell.com/shop
Size

Catalog Number

500 ml

C-40230

Dulbeccos PBS, w/o Ca /Mg

500 ml

C-40232

Dulbeccos PBS, w/o Ca++/Mg++, 10x conc.

500 ml

C-40238

++

++

n Hanks Balanced Salt Solutions


Product
Hanks BSS

https://www.promocell.com/shop
Size

Catalog Number

500 ml

C-40310

++

Hanks BSS, w/o Ca /Mg

500 ml

C-40320

Hanks BSS, phenol red-free

500 ml

C-40370

Hanks BSS, w/o Ca /Mg ,


phenol red-free

500 ml

C-40390

++

++

++

n Endotoxin-free Buffers and Water


Product

https://www.promocell.com/shop
Size

Catalog Number

PBS, endotoxin-free

500 ml

C-40240

PBS, w/o Ca /Mg , endotoxin-free

500 ml

C-40242

TC Water

500 ml

C-49998

++

++

n Other Solutions

https://www.promocell.com/shop

Product

Size

Catalog Number

CaCl2-Solution (0.5 M)

2 ml

C-34006

HEPES Buffered Saline (30 mM HEPES)

30 ml
125 ml
250 ml

C-40000
C-40010
C-40020

HEPES Buffer (1 M)

100 ml

C-40030

Sodium bicarbonate Solution (7.5%)

100 ml

C-42340

5 Reagents and Supplements

99

5.4 Supplements

n Growth Supplements
Size

Catalog Number

Endothelial Cell Growth Supplement*


(bovine hypothalamic extract)

2 ml
10 x 2 ml

C-30160
C-30180

Endothelial Cell Growth Supplement/


Heparin* (bovine hypothalamic extract)

2 ml
10 x 2 ml

C-30120
C-30140

Bovine Pituitary Extract 15*


(7.5 mg protein/ml)

2 ml
10 x 2 ml

C-30020
C-30040

Bovine Pituitary Extract 26*


(13 mg protein/ml)

2 ml
10 x 2 ml

C-30021
C-30041

50 mg

C-52310

Human Transferrin APO, low iron content

1g

C-52322

Human Transferrin HOLO, iron saturated

1g

C-52332

Human Insulin,
primary human cell culture tested

n Amino Acid Solutions


Product

https://www.promocell.com/shop
Size

Catalog Number

L-Glutamine Solution

100 ml

C-42210

Stable Glutamine Solution

100 ml

C-42215

MEM Amino Acids Solution

100 ml

C-42320

MEM NEAA Solution

100 ml

C-42322

n Vitamin and Trace Element Solutions


Product

https://www.promocell.com/shop
Size

Catalog Number

MEM Vitamin Solution

100 ml

C-42328

Trace Element Mix (1000x)

100 ml

C-42331

n Extracellular Matrix Proteins

https://www.promocell.com/shop

Product

Size

Catalog Number

Fibronectin Solution, bovine (1 mg/ml)

5 ml

C-43050

Fibronectin Solution, human (1 mg/ml)

5 ml

C-43060

* Endothelial Cell Growth Supplement (ECGS) and Bovine Pituitary Extract (BPE) are popular growth supplements in primary cell culture. PromoCell ECGS
and BPE are manufactured from raw material which is exclusively collected in New Zealand to ensure maximum BSE-safety.

Reagents and
Supplements

Product

https://www.promocell.com/shop

Cell Analysis
The specific analysis of cellular processes and conditions has
played an increasingly vital role in cell biology. Monitoring
reporter gene expression in cells, determining cell viability and
proliferation, testing for cytotoxicity, and distinguishing live
and dead cells are important procedures in most cell biology
labs. Measuring intracellular signaling and assaying distinct
cell metabolic activities have also become routine. These
applications all require reliable, sophisticated, and user-friendly
research tools.

102 Content Chapter 6

3
6

Cell Analysis
6.1 Cell Viability and Proliferation Kits . . . . . . 103
6.2 Live/Dead Cell Staining Kits . . . . . . . . . . . 105
6.3 Cytotoxicity Kits . . . . . . . . . . . . . . . . . . . . 106
6.4 Senescence Detection Kit . . . . . . . . . . . . . 106
6.5 Cell-Based Reporter Assays . . . . . . . . . . . 107
6.6 Cell Staining Reagents and

Related Products . . . . . . . . . . . . . . . . . . . 109
6.7 Intracellular Indicators and

Related Products . . . . . . . . . . . . . . . . . . . 115
6.8

Cell Signaling Assays . . . . . . . . . . . . . . . . 119

6.9

Cell Stress and Metabolism Kits . . . . . . . . 120

6.10 Cell Fractionation and



Protein Extraction Kits . . . . . . . . . . . . . . . 122
6.11 Protein Size Markers and

Protein Gel Stains . . . . . . . . . . . . . . . . . . . 123
6.12 Nucleic Acid Isolation Kits . . . . . . . . . . . . . 124
6.13 DNA Size Markers and DNA Gel Stains . . . 125

6 Cell Analysis

103

Applications



Cell viability and proliferation assays


Growth factor/cytokine assays
Optimization of cell culture conditions
Cell number determination

Features
Non-radioactive method
Easy and fast procedure
Reliable and reproducible results
Appropriate for various multiwell
formats and automation

PromoKine offers several types of cell
viability kits: The Fluorometric Cell
Viability Kits I and II; the Colorimetric
Cell Viability Kits I, II, III, and IV; and the
Bioluminescent Cell Viability Kits I and II.
Because all of the kits are designed for
use with various multiwell formats, they
are also suited for automation.

Fluorometric Cell Viability Kit I


(Resazurin)

Fluorometric Cell Viability Kit II


(Calcein)

The resazurin-based PromoKine Cell


Viability Kit I provides a simple and rapid
measurement of cell viability. Moreover,
this single-step, homogenous cell proliferation assay is sensitive, safe, and costeffective. It only requires as few as 80
cells for reproducible results. The assay is
based on the ability of viable cells to convert the non-fluorescent, blue redox dye
Resazurin (also known as Alamar Blue)
into the highly red fluorescent, pink dye
resorufin.The fluorescent or colorimetric
signal generated from the assay is proportional to the number of living cells in
the sample. The kit reagents are nontoxic to cells and allow kinetic monitoring.
While fluorometric resorufin quantitation is more sensitive, colorimetric measurement is also possible.

The PromoKine Fluorometric Cell Viability Kit II is intended for the reliable
fluorometric determination of viable cell
numbers. It utilizes the cell membranepermeable, non-fluorescent dye CalceinAM that is hydrolyzed by esterases into
the cell membrane-impermeable greenfluorescent Calcein (Fig. 1). Since esterases are only active in viable cells, the
amount of Calcein-AM hydrolyzed by
cellular esterases is directly proportional
to the number of viable cells in culture.
The kit has a detection range from less
than 50 to more than 25,000 cells per
well.

The Colorimetric Cell Viability Kits I and


II allow for easy and reliable colorimetric determination of viable cell numbers
with excellent sensitivity and linearity. The kits utilize the tetrazolium salts
WST-8 (Kit I) and WST-1 (Kit II) that are
reduced to water-soluble, orange formazan dyes by dehydrogenases present in
viable cells (see Fig. 2). The absorbance
of the formazan dye is proportional to
the number of metabolically active cells
and can be measured directly from a 96well plate without additional processing.

Calcein-AM
R: acetoxymethyl
Esterase

Viable cell

Colorimetric Cell Viability Kits I


(WST-8) and II (WST-1)

Calcein

Colorimetric Cell Viability Kits III


(XTT) and IV (MTT)
Fig. 1: Assay mechanism of Calcein-AM conversion by intracellular esterases.

WST-8 Formazan
orange dye

WST-8
slightly yellow

1-Methoxy PMS

Viable cell

NADH, NADPH
Dehydrogenase
NAD, NADP

Fig. 2: Assay mechanism of WST-8 conversion by dehydrogenases in viable cells.

The PromoKine Colorimetric Cell Viability Kits III and IV were developed for
determining the number of viable cells in
culture. They are based on the reduction
of the tetrazolium salts XTT or MTT into
colored formazan compounds by mitochondrial enzymes. Since these enzymes
are inactivated shortly after cell death,
this is a reliable method for the detection of viable cells. The absorbance of
the formazan product can be measured
directly from a 96-well plate without additional processing.

Cell Analysis

6.1 Cell Viability and Proliferation Kits

Cell Analysis

104

6 Cell Analysis

Bioluminescent Cell Viability Kit I


(ATP)

Bioluminescent Cell Viability Kit II


(ADP/ATP)

The Promokine Bioluminescent Cell


Viability Kit I employs bioluminescence
to measure the ATP level as a marker for
cell viability in mammalian cells. It utilizes
luciferase to catalyze the ATP dependent, light-producing oxidation of luciferin. This light formation can be detected
using a luminometer or beta counter.
The assay can be performed directly in
culture plates requiring no harvesting,
washing, or sample preparation and can
be fully automated for high throughput
(10 seconds/sample). It is highly sensitive, detecting 10-100 mammalian cells
per well. It also offers applications besides cell viability testing, e.g., detecting low level bacterial contamination in
blood, milk, urine, soil, and sludge.

The ADP/ATP ratio is a good indicator for


cell viability. High ATP and low ADP levels are usually found in proliferating cells.
In contrast, decreased levels of ATP and
increased levels of ADP are characteristic for apoptotic cells. In necrotic cells,
the ADP levels are even higher, and ATP
can be barely detected. The Bioluminescent Cell Viability Kit II utilizes bioluminescence to measure the ADP/ATP ratio
providing an innovative system for fast,
simultaneous screening of cell proliferation, apoptosis, necrosis, and growth arrest in mammalian cells. The ATP level is
determined using the luciferin-luciferase
reaction used in the Bioluminescent Cell
Viability Kit I. The ADP level is then measured by its conversion to ATP, which is
subsequently detected using the same
luciferase-catalyzed reaction. The assay works with full automation for high
throughput, detects as few as 100 mammalian cells per well, and offers highly
consistent results with excellent correlation to other apoptosis markers (e.g.,
TUNEL-based or caspase assays).

Cell Proliferation Kit I (CFSE)


PromoKines Cell Proliferation Kit I
provides a convenient and sensitive
way to determine cell proliferation or
cell division. The assay is based on the
amine-reactive dye CFDA SE (5-(and
6)-carboxyfluorescein diacetate, succinimidyl ester; also known as CFSE) which
is non-fluorescent and can diffuse passively into cells. When it enters the cells,
the acetate group is removed by intracellular esterases yielding the bright greenfluorescent dye carboxyfluorescein SE.
Its succinimidyl ester (SE) reactive group
reacts with intracellular amines and attaches the fluorescent dye to intracellular
proteins, retaining it in the cells and allowing fixation with aldehyde fixatives.
CFDA SE has been used as a tracer for
several cell divisions as the label is stably
inherited by daughter cells through successive cell divisions or after cell fusion
and is not transferred to adjacent cells in
a population.
The kit is compatible with flow cytometry and microscopy and contains 10
single-use vials as well as a detailed protocol for cellular labeling.

https://www.promokine.info/shop
Product

Size

Catalog Number

500 assays
2500 assays
10000 assays

PK-CA707-30025-0
PK-CA707-30025-1
PK-CA707-30025-2

1000 assays

PK-CA707-30026

Colorimetric Cell Viability Kit I (WST-8)

100 assays
500 assays

PK-CA705-CK04-100
PK-CA705-CK04

Colorimetric Cell Viability Kit II (WST-1)

500 assays
2500 assays

PK-CA577-K304-500
PK-CA577-K304-2500

Colorimetric Cell Viability Kit III (XTT)

200 assays
1000 assays

PK-CA20-300-200
PK-CA20-300-1000

Colorimetric Cell Viability Kit IV (MTT)

200 assays
1000 assays

PK-CA707-30006-200
PK-CA707-30006

Bioluminescent Cell Viability Kit I (ATP)

200 assays
1000 assays

PK-CA577-K254-200
PK-CA577-K254-1000

Bioluminescent Cell Viability Kit II (ADP/ATP)

200 assays

PK-CA577-K255

Cell Proliferation Kit I (CFSE)

10 x 50 g

PK-CA707-30050

10 mg

PK-CA707-10054

Fluorometric Cell Viability Kit I (Resazurin)

Fluorometric Cell Viability Kit II (Calcein)

Resazurin, sodium salt

Note: Please check our website regularly for new cell-based assays.

6 Cell Analysis

105

6.2 Live/Dead Cell Staining Kits

Monitoring of viable and dead cells


Cell counting by fluorescence micros copy and fluor cytometry

Features






Simultaneous monitoring of viable


and dead cells
High fluorescent intensity
Fast and convenient procedure
Excitation and emission spectra are
compatible with common excitation
sources and filter sets

Live/Dead Cell Staining Kit II


The Live/Dead Cell Staining Kit II utilizes
two fluorescent dyes, Calcein-AM and

Calcein-AM
R: acetoxymethyl

Ethidium homodimer III (EthD-III). Calcein-AM penetrates the cell membrane


of living cells. Once inside, the cellular
esterases present in living cells hydrolyze it to cell membrane-impermeable,
green-fluorescent Calcein (emission
maximum at 515 nm).
In contrast, EthD-III can only pass
through the disrupted membrane areas
of dead cells where it intercalates with
the DNA of the nucleus, emitting red
light at 620 nm. Compared with PI,
EthD-III is more stable and emits brighter
fluorescent signals. Since both Calcein
and EthD-III can be excited at 490 nm,
a simultaneous monitoring of viable and
dead cells is possible using a fluorescence
microscope (see Fig. 4). At an excitation
of 545 nm, the number of dead cells can
be monitored.

Bacteria Live/Dead Staining Kit


The Bacteria Live/Dead Staining Kit enables a two-color fluorescent staining
on both live bacteria (green) and dead
bacteria (red) using the two nucleic
acid dyes DMAO and EthD-III. DMAO
is a green-fluorescent dye which stains
both live and dead bacteria with intact
and damaged cell membranes while
the red-fluorescing EthD-III only stains
dead bacteria with damaged cell membranes. With an appropriate mixture of
both dyes, viable bacteria with intact cell
membranes are stained green, whereas
bacteria with damaged cell membranes
fluoresce red. The kit is applicable to
most bacteria types for viability and
cytotoxicity studies and is suitable for
fluorescence microscopy and flow cytometry.

EthD-III

Esterase

Viable cell

DNA intercalation
red fluorescence

Fig. 4: Double-staining of viable


(green) and dead (red) cells using the
PromoKine Live/Dead Cell Staining
Kit II.

Calcein
green fluorescence

Fig. 3: Assay mechanism of the PromoKine Live/Dead Cell Staining Kit II.

https://www.promokine.info/shop
Product

Size

Catalog Number

Live/Dead Cell Staining Kit II

300-1000 assays

PK-CA707-30002

Bacteria Live/Dead Staining Kit

100-1000 assays

PK-CA707-30027

Please see also the PromoKine Apoptotic/Necrotic/Healthy Cells Detection Kit on page 140 and our individual stains for viable
and dead cells on page 110.
Note: Please check our website regularly for new cell-based assays.

Cell Analysis

Applications

106

6 Cell Analysis

6.3 Cytotoxicity Kits


Applications
Cytotoxicity assays
Chemosensitivity testing
Toxicity screening

Cell Analysis

Features



Non-radioactive method
Highly sensitive
Easy and fast procedure
Compatible with common plate readers

It oxidizes lactate to pyruvate, triggering


the conversion of the yellow tetrazolium
salt WST into the water-soluble red
formazan dye.
The increase in the amount of formazan
produced in culture medium can be
analyzed spectrophotometrically and
correlates directly to the increase in the
number of lysed cells.
The kit can be used for many different in
vitro cell systems to quantify cytotoxicity
and cytolysis.

LDH Cytotoxicity Kit II

AK Cytotoxicity Kit

The colorimetric LDH Cytotoxicity Kit


II provides a fast, sensitive, and precise
alternative to the radioactive [3H]-thymidine and [51Cr] release assays. It is based
on the activity of lactate dehydrogenase
(LDH), a stable cytoplasmic enzyme that
is present in all cells. Damaged cells
rapidly release this enzyme into the surrounding medium. LDH activity is determined by a coupled enzymatic reaction.

The bioluminometric AK Cytotoxicity Kit


is based on the measurement of adenylLDH

Lactate
NAD+
Formazan
(red)

ate kinase (AK), a ubiquitous protein


present in all cells that is rapidly released
into the culture medium upon damage
to the plasma membrane. The simple
one-step procedure involves two chemical reactions. The adenylate kinase release from the damaged cells catalyses
the conversion of ADP to ATP. The luciferase catalyses a light-producing reaction between the ATP, oxygen, and luciferin. The light can be measured using a
luminometer or beta counter. This highly
sensitive assay allows the rapid quantitation of plasma membrane damage for
evaluation of cell death or cytotoxicity
events and it is well suited for automation and high throughput.

Pyruvate
NADH + H+

Catalyst

WST-1
(yellow)

Fig. 5: Assay mechanism of the PromoKine LDH Cytotoxicity Kit II.


https://www.promokine.info/shop
Product

Size

Catalog Number

LDH Cytotoxicity Kit II

500 assays

PK-CA577-K313

AK Cytotoxicity Kit

500 assays

PK-CA577-K312

6.4 Senescence Detection Kit


Applications
Histochemical detection of senescent
cells
Cell senescence and oncology studies

Features
High sensitivity and specificity
Compatible with common lab
equipment

Senescence is an arrested state in which


the cells remain viable but are not stimulated to divide by serum or passage in
culture. It is thought to be a tumor-suppressive mechanism and an underlying
cause of aging. Senescent cells display an
increase in size, senescence-associated
expression of b-galactosidase (SA-b-Gal)
activity, and altered patterns of gene
expression. The PromoKine Senescence

Detection Kit has been designed to histochemically detect a specific senescence


marker (SA-b-Gal activity) in cultured
cells and tissue sections.
The specific histochemical marker is
only present in senescent cells and is not
found in presenescent, quiescent, or immortal cells.

https://www.promokine.info/shop
Product
Senescence Detection Kit

Note: Please check our website regularly for new cell-based assays.

Size

Catalog Number

250 assays

PK-CA577-K320

6 Cell Analysis

107

6.5 Cell-Based Reporter Assays

Monitoring and quantification of


reporter gene expression (Luciferase,
b-Galactosidase, GFP)
Determination of transfection
efficiencies

Features



Highly sensitive and reproducible


Fast and convenient protocols
Compatible with common
plate readers

under these conditions, light emitted


from the reaction is directly proportional
to the number of luciferase enzyme
molecules. The light emission from the
luciferase reaction can be recorded using
a luminometer.
The Luciferase Reporter HTS Kits have
been specifically designed for economic
high-throughput applications using
high-density microplates.
In addition to the Firefly luciferase substrate D-Luciferin, we also provide a
wide range of coelenterazine substrates
for the Renilla luciferase catalysed reaction.

Luciferase Reporter Assay Kits


GFP Reporter Assay Kit
The PromoKine ready-to-use Luciferase
Reporter Assays provide complete systems for rapid, convenient, and quantitative detection of luciferase activity in
eukaryotic cells, e.g. in cells transfected
with plasmids carrying the Firefly and/
or Renilla luciferase reporter genes. They
are optimized to provide excellent sensitivity as well as consistent light output
when working with multiple samples.
Firefly luciferase catalyzes the oxidative
carboxylation of luciferin, a reaction
with the highest efficiency of any known
bioluminescence reaction. Consequently,
this sensitive reporter is ideally suited for
studying gene regulation and function.
Renilla luciferase is often used in conjunction with Firefly luciferase as a
normalizing transfection control or for
multiplexing transcriptional reporter assays. Both Firefly luciferase and Renilla
luciferase show maximum light output
when substrates and co-factors are present in large quantities. When assayed

GFP (Green Fluorescent Protein) is a fluorescent protein originally isolated from


the jellyfish Aequorea victoria. GFP and
its enhanced variant EGFP have been
widely used as reporter proteins for in
vivo monitoring of gene expression in a
variety of cell types and organisms. Since
GFP requires no additional substrates or
cofactors, GFP fluorescence can easily
be detected using a fluorescence microscope. However, most imaging studies of GFP are qualitative. Quantitative
analyses of GFP expression levels in cells
or tissues are more informative and have
wider applications.
The PromoKine GFP Reporter Assay Kit
was designed for use in a 96-well microplate format (Ex/Em = 488 nm/507nm).
Cells or tissues can be homogenized directly in the provided GFP assay buffer
and the supernatant of the lysed cells/
tissues is transfered to the microwell
plates. The amount of GFP is determined

by comparing its fluorescence with that


of a GFP standard. The kit can detect a
wide range of GFP concentrations (0.0110 g/ml). Furthermore, a GFP quench
solution is provided for determining
autofluorescence levels of cell or tissue
extracts.

Beta-Galactosidase Kits
The b-Galactosidase Staining Kit and
the b-Galactosidase Reporter Assay Kit
were designed to detect and quantify
b-Gal activity in cells transfected with
a plasmid carrying the frequently-used
LacZ reporter gene. This gene encodes
b-galactosidase, an enzyme that is very
stable and resistant to proteolytic degradation. When expressed in transfected
cells, it converts a colorless substrate
(e.g., CPRG, X-Gal, or ONPG) into a
colored reaction product that can be
easily detected microscopically (b-Galactosidase Staining Kit) or quantified using
a spectrophotometer (b-Galactosidase
Reporter Assay Kit). In addition, PromoKine provides a range of chromogenic
beta-Galactosidase substrates.

Fig. 6: b-Gal staining of CHO-K1 cells


transfected with the pPK-CMV-R1 reporter vector expressing the lacZ gene
(see page 166).

https://www.promokine.info/shop
Product

Size

Catalog Number

Luciferase Reporter Assay Kit I (Firefly)

150 assays
1000 assays

PK-CA707-30003-1
PK-CA707-30003-2

Luciferase Reporter Assay Kit II (Renilla)

150 assays
1000 assays

PK-CA707-30004-1
PK-CA707-30004-2

Luciferase Reporter Assay Kit III


(Firefly & Renilla)

100 assays
1000 assays

PK-CA707-30005-1
PK-CA707-30005-2

Luciferase Reporter HTS Kit (Firefly)

4 ml
10 ml
100 ml
10 x 100 ml

PK-CA707-30028-0
PK-CA707-30028-1
PK-CA707-30028-2
PK-CA707-30028-3

Cell Analysis

Applications

108

6 Cell Analysis

https://www.promokine.info/shop
Product

Size

Catalog Number

beta-Galactosidase Reporter Assay Kit

500 assays

PK-CA708-10100

beta-Galactosidase Staining Kit

200 assays

PK-CA577-K802

GFP Reporter Assay Kit

100 assays

PK-CA577-K815

D-Luciferin, Free Acid

10 mg
50 mg
1g

PK-CA707-10100
PK-CA707-10100-1
PK-CA707-10100-2

D-Luciferin, Potassium Salt

10 mg
50 mg
1g

PK-CA707-10101
PK-CA707-10101-1
PK-CA707-10101-2

D-Luciferin, Sodium Salt

10 mg
50 mg
1g

PK-CA707-10102
PK-CA707-10102-1
PK-CA707-10102-2

5 mg

PK-CA707-10103

Coelenterazine (native)

50 g

PK-CA707-10110

Coelenterazine h

50 g

PK-CA707-10111

Coelenterazine cp

50 g

PK-CA707-10112

Coelenterazine hcp

50 g

PK-CA707-10113

Coelenterazine f

50 g

PK-CA707-10114

Coelenterazine n

50 g

PK-CA707-10115

Coelenterazine ip

50 g

PK-CA707-10116

Coelenterazine fcp

50 g

PK-CA707-10117

Coelenterazine i

50 g

PK-CA707-10121

Coelenterazine, 2-methyl

50 g

PK-CA707-10122

Coelenterazine 400a
(also known as DeepBlue C,
a trademark of Perkin Elmer)

50 g

PK-CA707-10125

1 Kit

PK-CA707-10123

X-Gal

100 mg
1g
5g

PK-CA707-10011
PK-CA707-10011-1
PK-CA707-10011-2

Red-beta-D-Gal

100 mg

PK-CA707-10012

Rose-beta-D-Gal

100 mg

PK-CA707-10013

25 mg

PK-CA707-10014

Cell Analysis

Firefly Luciferase Substrates

DMNPE-caged D-Luciferin
Renilla Luciferase Substrates

Coelenterazine Sampler Kit


Chromogenic beta-Galactosidase Substrates

Purple-beta-D-Gal
Green-beta-D-Gal
Chromogenic beta-Galactosidase
Substrate Sampler Kit

25 mg

PK-CA707-10015

1 kit (5 x 5 mg)

PK-CA707-10024

Please see page 166 and www.promokine.info for luciferase, beta-Gal, and GFP reporter vectors.
Note: Please check our website regularly for new cell-based assays.

6 Cell Analysis

109

6.6 Cell Staining Reagents and Related Products

Detection of cellular structures


Fluorescence microscopy, Flow
cytometry

Features
High quality
Thoroughly tested
Highly sensitive
Fluorescent dyes offer extraordinary sensitivity and extended possibilities for the
visualization and analysis of intracellular
processes and cell structures such as the
cell membrane, mitochondria, the nucleus, and the cytoskeleton. Some dyes

are also suited for staining only viable


or dead cells. That is an important feature for elucidating the effects of certain
agents on cell viability.
PromoKine provides a select choice of
reagents for staining different cell organelles and structures as well as for
determining processes like membrane
potential changes. Some of these reagents are available as esters, which pass
through the membranes of viable cells.
Once inside, the ester group is hydrolyzed by cellular esterases, and the dye
can accumulate in the cell. Some dyes
are nonfluorescent in their ester form but
fluoresce strongly when the ester group
has been removed.

Fig. 7: Primary human fibroblasts (NHDF,


see page 27) stained with Calcein and
counterstained with DAPI.

https://www.promokine.info/shop
Product

Product Description

Size

Catalog Number

1 mg

PK-CA707-40037

10 ml
(10 mg/ml)

PK-CA707-40039

10 mg

PK-CA707-40011

10 mg in 1 ml

PK-CA707-40043

10 mg

PK-CA707-40009

Nucleus Stains
7-AAD
(7-aminoactinomycin D)

Fluorescent DNA intercalator

Acridine Orange (AO)


Solution

Nucleus stain (DNA/RNA)

DAPI

Nucleus stain (DNA)

DAPI Solution

Nucleus stain (DNA); aqueous solution

DAPI, dilactate

4,6-diamidino-2-phenylindole, dilactate

DMAO

Nucleus stain (DNA); 2 mM solution in DMSO

EB (ethidium bromide)
Solution

Dead cell stain (DNA), aqueous solution

Ethidium Homodimer I

Nucleus stain (DNA); also known as EthD-1, or EtDi

Ethidium Homodimer III

Nucleus stain (DNA)

Ethidium Homodimer III,


1 mM in DMSO

Nucleus stain (DNA); in DMSO

Hoechst 33258

Nucleus stain (DNA)

Hoechst 33258 Solution

Nucleus stain (DNA); aqueous solution

Hoechst 33342

Nucleus stain (DNA)

Hoechst 33342 Solution

Nucleus stain (DNA); aqueous solution

Propidium Iodide (PI)

Dead cell stain (DNA)

Propidium Iodide (PI) Solution

Dead cell stain (DNA); aqueous solution

1 ml

PK-CA707-40012

10 ml
(10 mg/ml)

PK-CA707-40042

1 mg

PK-CA707-40010

1 mg

PK-CA707-40050

200 l

PK-CA707-40051

100 mg

PK-CA707-40045

10 ml
(10 mg/ml)

PK-CA707-40044

100 mg

PK-CA707-40047

10 ml
(10 mg/ml)

PK-CA707-40046

100 mg

PK-CA707-40016

10 ml
(1 mg/ml)

PK-CA707-40017

Mitochondria Stains
DASPEI

2-(4-(dimethylamino)styryl)-N-ethylpyridinium iodide;
for staining of mitochondria in live cells

100 mg

PK-CA707-70018

Dihydrorhodamine 123

Reduced form of Rhodamine 123; mitochondria stain

10 mg

PK-CA707-10055

Dihydrorhodamine 123,
dihydrochloride salt

Reduced form of Rhodamine 123; mitochondria stain

10 mg

PK-CA707-10056

DiOC6(3)

3,3-dihexyloxacarbocyanine, iodide; membrane


potential sensitive dye

100 mg

PK-CA707-70009

Cell Analysis

Applications

110

6 Cell Analysis

Cell Analysis

https://www.promokine.info/shop
Product

Product Description

Size

Catalog Number

JC-1 chloride

Stains mitochondria in living cells in a membrane


potential-dependent function

5 mg

PK-CA707-70011

JC-1 iodide

Stains mitochondria in living cells in a membrane


potential-dependent function

5 mg

PK-CA707-70014

Nonyl Acridine Orange


(or NAO)

Mitochondria stain; not dependent on mitochondrial


membrane potential

50 mg

PK-CA707-70012

MitoGreen

Green-fluorescent mitochondrial dye

20 x 50 g

PK-CA707-70054

MitoRed

Red-fluorescent mitochondrial dye

20 x 50 g

PK-CA707-70055

Rhodamine 123

Green-fluorescent mitochondria stain

50 mg

PK-CA707-70010

Tetrabromorhodamine 123,
bromide

Green-fluorescent mitochondria stain

5 mg

PK-CA707-70013

TMRE

Tetramethylrhodamine ethyl ester, perchlorate;


membrane potential sensitive dye

25 mg

PK-CA707-70016

TMRM

Tetramethylrhodamine methyl ester, perchlorate;


membrane potential sensitive dye

25 mg

PK-CA707-70017

100 mg

PK-CA707-70009

ER Stains
DiOC6(3)

3,3-dihexyloxacarbocyanine, iodide;
membrane potential sensitive dye

Viable Cell Stains


BCECF

Viable cell stain (cytosol)

1 mg

PK-CA707-51010

BCECF AM

Viable cell stain (cytosol); acetoxymethyl ester

10 x 100 g
20 x 50 g
1 mg

PK-CA707-51011
PK-CA707-51011-1
PK-CA707-51012

BCECF AM Solution

Viable cell stain (cytosol); acetoxymethyl ester;


DMSO solution

1 mg in 1 ml

PK-CA707-51009

Calcein AM

Viable cell stain (cytosol); acetoxymethyl ester

20 x 50 g
1 mg

PK-CA707-80011-3
PK-CA707-80011

Calcein AM Solution,
1 mg/ml in anhydrous DMSO

Viable cell stain (cytosol); acetoxymethyl ester,


DMSO solution

1 mg in 1 ml

PK-CA707-80011-2

Calcein AM Solution,
4 mM DMSO solution

Viable cell stain (cytosol); acetoxymethyl ester,


4 mM DMSO solution

100 l

PK-CA707-80011-1

Calcein, high purity

Viable cell stain (cytosol); ultra-pure

100 mg

PK-CA707-80013

CFSE; 5(6)-CFDA, SE

5-(and-6)-carboxyfluorescein diacetate,
succinimidyl ester; viable cell stain (cytosol)

25 mg

PK-CA707-90041

Hoechst 33258

Nucleus stain (DNA)

100 mg

PK-CA707-40045

Hoechst 33258 Solution

Nucleus stain (DNA); aqueous solution

10 ml
(10 mg/ml)

PK-CA707-40044

Hoechst 33342

Nucleus stain (DNA)

100 mg

PK-CA707-40047

Hoechst 33342 Solution

Nucleus stain (DNA); aqueous solution

10 ml
(10 mg/ml)

PK-CA707-40046

EB (ethidium bromide)
Solution

Dead cell stain (DNA); aqueous solution

10 ml
(10 mg/ml)

PK-CA707-40042

Ethidium Homodimer I

Nucleus stain (DNA); also known as EthD-1, or EtDi

1 mg

PK-CA707-40010

Ethidium Homodimer III

Nucleus stain (DNA)

1 mg

PK-CA707-40050

Ethidium Homodimer III,


1 mM in DMSO

Nucleus stain (DNA); in DMSO

200 l

PK-CA707-40051

Propidium Iodide (PI)

Dead cell stain (DNA)

Propidium Iodide (PI) Solution

Dead cell stain (DNA); aqueous solution

Trypan Blue Solution (0.5%)

Dead cell stain; tetrasodium salt, 0.5% solution

Dead Cell Stains

100 mg

PK-CA707-40016

10 ml (1 mg/ml)

PK-CA707-40017

10 ml

PK-CA902-1209

6 Cell Analysis

111

https://www.promokine.info/shop
Product

Product Description

Size

Catalog Number

Blue-fluorescent Cytoplasmic
Membrane Staining Kit

Convenient and sensitive cell staining


with low cytotoxicity

50 assays

PK-CA707-30024

Green-fluorescent Cytoplasmic Membrane Staining Kit

Convenient and sensitive cell staining


with low cytotoxicity

1 ml

PK-CA707-30021

Orange-fluorescent Cytoplasmic Membrane Staining Kit

Convenient and sensitive cell staining


with low cytotoxicity

1 ml

PK-CA707-30022

Red-fluorescent Cytoplasmic
Membrane Staining Kit

Convenient and sensitive cell staining


with low cytotoxicity

1 ml

PK-CA707-30023

Lipophilic Carbocyanine Dyes


DiD (DiIC18[5])

1,1'-dioctadecyl-3,3,3',3'- tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt

50 mg

PK-CA707-60014

DiI (or DiIC18[3])

1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine
perchlorate

50 mg

PK-CA707-60010

DiI in vegetable oil

Optimized DiI formulation for microinjection

0.5 ml

PK-CA707-60018

Dilinoleyl DiI

1,1'-dilinoleyl-3,3,3',3'-tetramethylindocarbocyanine
perchlorate (also called Fast DiI, a trademark of
Molecular Probes)

5 mg

PK-CA707-60034

Dilinoleyl DiO

1,1'-dilinoleyl-3,3'-oxacarbocyanine perchlorate
(also called Fast DiO, a trademark of Molecular Probes)

5 mg

PK-CA707-60035

DiO (or DiOC18[3])

3,3'-dioctadecyloxacarbocyanine, perchlorate

50 mg

PK-CA707-60011

DiOC14(3),
hydroxyethanesulfonate

3,3'-ditetradecyloxacarbocyanine,
hydroxyethanesulfonate

50 mg

PK-CA707-60012

DiOC16(3)

Lipophilic cyanine dye for staining cytoplasmic


membranes

25 g

PK-CA707-60038

DiR (DiIC18[7])

1,1'-dioctadecyl-3,3,3',3'-tetramethyl
indotricarbocyanine Iodide

25 mg

PK-CA707-60017

Neuro-DiI

Improved alternative to DiI

25 mg

PK-CA707-60016

Neuro-DiI in vegetable oil

Optimized formulation for microinjection

0.2 ml

PK-CA707-60020

Neuro-DiO

Improved alternative to DiO

25 mg

PK-CA707-60015

Neuro-DiO in vegetable oil

Optimized formulation for microinjection

0.2 ml

PK-CA707-60019

Biotin-DHPE

N-(biotinoyl)-1,2-dihexadecanoyl-sn-glycero-3phosphoethanolamine, triethylammonium salt

10 mg

PK-CA707-60022

Biotin-X-DHPE

N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine, triethylammonium
salt

5 mg

PK-CA707-60023

Fluorescein-DHPE

N-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-snglycero-3-phosphoethanolamine, triethylammonium salt

5 mg

PK-CA707-60024

NBD-DHPE

N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt

10 mg

PK-CA707-60025

Rhodamine-DHPE

N-(lissamine rhodamine B sulfonyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine, triethylammonium


salt

5 mg

PK-CA707-60026

Texas Red-DHPE

N-(Texas Red sulfonyl)-1,2-dihexadecanoyl-sn-glycero3-phosphoethanolamine, triethylammonium salt

1 mg

PK-CA707-60027

TRITC-DHPE

N-(teramethylrhodamine-6-thiocarbamoyl)-1,2dihexadecanoyl-sn-glycero-3-phosphoethanolamine,
triethylammonium salt

1 mg

PK-CA707-60028

Lipophilic styryl dye with an amine group at the


hydrophilic head

1 mg

PK-CA707-70051

Phospholipid Membrane Probes

Other Membrane Dyes


AM3-25

Cell Analysis

Cytoplasmic Membrane Staining

112

6 Cell Analysis

Cell Analysis

https://www.promokine.info/shop
Product

Product Description

Size

Catalog Number

Biotin-AM3-25

AM3-25 conjugate with biotin that can be coupled


to avidin or streptavidin

1 mg

PK-CA707-60032

DiA

4-(4-dihexadecylaminostyryl)-N-methylpyridinium iodide

50 mg

PK-CA707-60013

DiB

Lipophilic blue fluorescent dye useful for staining


cytoplasmic membranes

10 g

PK-CA707-60036

DPA

Nonfluorescent anionic membrane dye that can be used


as a FRET quencher for a variety of fluorescent donor
dyes

25 g

PK-CA707-60037

NBD C6-ceramide

6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)
hexanoyl)sphingosine

1 mg

PK-CA707-60030

NBD C6-sphingomyelin

6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)
hexanoyl)sphingosyl phosphocholine

1 mg

PK-CA707-60031

Octadecyl Rhodamine B
Chloride (R18)

Useful for membrane fusion assays

10 mg

PK-CA707-60033

Nerve Terminal Staining Kits


Nerve Terminal Green
Staining Kit I

Kit contains 5 x 1 mg SynapseGreen C4


and 250 mg ADVASEP-7

1 set

PK-CA707-70030

Nerve Terminal Green


Staining Kit II

Kit contains 1 mg AM1-43 and 100 mg ADVASEP-7

1 set

PK-CA707-70031

Nerve Terminal Green


Staining Kit III

Kit contains 1 mg AM1-43 and 100 mg SCAS

1 set

PK-CA707-70031-1

Nerve Terminal Green


Staining Kit IV

Kit contains 5 x 1 mg SynapseGreen C4


and 100 mg Sulforhodamine 101

1 set

PK-CA707-70032

Nerve Terminal Red


Staining Kit I

Kit contains 5 x 1 mg SynapseRed C2


and 250 mg ADVASEP-7

1 set

PK-CA707-70034

Nerve Terminal Red


Staining Kit II

Kit contains 5 x 1 mg SynapseRed C2


and 100 mg Sulforhodamine 101

1 set

PK-CA707-70035

250 mg

PK-CA707-70029

Nerve Terminal Probes & Related Reagents


ADVASEP-7
(sulfobutyl b-cyclodextrin)

For background fluorescence reduction

Alpha-Bungarotoxin
(from Bungarus multicinctus)

Acetylcholine receptor inhibitor

1 mg

PK-CA707-00010-1

AM1-43

Fixable, activity-dependent fluorescent nerve


terminal probe

1 mg

PK-CA707-70024

AM1-44

Nerve Terminal Probe

1 mg

PK-CA707-70038

AM2-10

Nerve Terminal Probe

1 mg

PK-CA707-70036

AM3-25

Nerve Terminal Probe

1 mg

PK-CA707-70051

AM4-64

Nerve Terminal Probe

1 mg

PK-CA707-70025

AM4-66

Nerve Terminal Probe

1 mg

PK-CA707-70050

Biotin-XX-a-Bungarotoxin

Can be used to isolate and/or image the distribution of


the nicotinic acetylcholine receptor at the neuromuscular
junctions

500 g

PK-CA707-00017

Fluorescein-a-Bungarotoxin

Can be used to image the distribution of the nicotinic


acetylcholine receptor at the neuromuscular junctions

500 g
10 x 50 g

PK-CA707-00011
PK-CA707-00013

SCAS

4-sulfonato calix[8]arene, sodium salt; for background


fluorescence reduction

100 mg

PK-CA707-70037

Sulforhodamine 101-aBungarotoxin

Also known as Texas Red-a-Bungarotoxin

500 g
10 x 50 g

PK-CA707-00015
PK-CA707-00016

SynapseGreen C1

Nerve Terminal Probe

5 mg
5 x 1 mg

PK-CA707-70042
PK-CA707-70043

SynapseGreen C18
(FM3-25)

Nerve Terminal Probe (also known as FM3-25,


a trademark of Molecular Probes, Inc.)

5 mg
5 x 1 mg

PK-CA707-70048
PK-CA707-70049

SynapseGreen C2 (FM2-10)

Nerve Terminal Probe (also known as FM2-10,


a trademark of Molecular Probes, Inc.)

5 mg
5 x 1 mg

PK-CA707-70044
PK-CA707-70045

6 Cell Analysis

113

Product

Product Description

Size

Catalog Number

SynapseGreen C3

Nerve Terminal Probe

5 mg
5 x 1 mg

PK-CA707-70023
PK-CA707-70026

SynapseGreen C4 (FM1-43)

Nerve Terminal Probe (also known as FM1-43,


a trademark of Molecular Probes, Inc.)

5 mg
5 x 1 mg

PK-CA707-70020
PK-CA707-70022

SynapseGreen C5 (FM1-84)

Nerve Terminal Probe (also known as FM1-84,


a trademark of Molecular Probes, Inc.)

5 mg
5 x 1 mg

PK-CA707-70046
PK-CA707-70047

SynapseRed C1

Nerve Terminal Probe

5 mg
5 x 1 mg

PK-CA707-70040
PK-CA707-70041

SynapseRed C2 (FM4-64)

Nerve Terminal Probe (also known as FM4-64, a trademark of Molecular Probes, Inc.)

5 mg
5 x 1 mg

PK-CA707-70021
PK-CA707-70027

SynapseRed C2M (FM5-95)

Nerve Terminal Probe (also known as FM5-95, a trademark of Molecular Probes, Inc.)

5 mg
5 x 1 mg

PK-CA707-70028
PK-CA707-70019

Tetramethylrhodaminea-Bungarotoxin

Can be used to image the distribution of the nicotinic


acetylcholine receptor at the neuromuscular junctions

500 g
10 x 50 g

PK-CA707-00012
PK-CA707-00014

Membrane Potential Sensitive Dyes


DiO/DPA Membrane
Potential Detection Kit

For fluorescent detection of cytoplasmic


membrane potential changes

1 kit

PK-CA707-30037

Di-12-ANEPPQ

Fast-responding membrane potential dye

5 mg

PK-CA707-61015

Di-2-ANEPEQ (JPW1114)

Fast-responding membrane potential dye;


also called JPW1114

5 mg

PK-CA707-61013

Di-4-ANEPPS

Fast-responding membrane potential dye

5 mg

PK-CA707-61010

Di-8-ANEPPQ

Fast-responding membrane potential dye

5 mg

PK-CA707-61014

Di-8-ANEPPS

Fast-responding membrane potential dye

DiBAC4(3)

Bis-(1,3-dibarbituric acid)-trimethine oxanol;


membrane potential sensitive dye

DiIC1(5)

5 mg

PK-CA707-61012

25 mg

PK-CA707-61011

1,1'-dimethyl-3,3,3',3'-tetramethylindodicarbocyanine,
iodide

100 mg

PK-CA707-70015

DiOC2(3)

3,3-diethyloxacarbocyanine, iodide;
membrane potential sensitive dye

100 mg

PK-CA707-70008

DiOC5(3)

3,3-dipentyloxacarbocyanine, iodide;
membrane potential sensitive dye

100 mg

PK-CA707-70007

DiOC6(3)

3,3-dihexyloxacarbocyanine, iodid;
membrane potential sensitive dye

100 mg

PK-CA707-70009

RH237

Fast-responding potentiometric probe

5 mg

PK-CA707-61018

RH414

Fast-responding potentiometric probe

5 mg

PK-CA707-61016

RH421

Fast-responding membrane potential dye

25 mg

PK-CA707-61017

RH795

Fast-responding potentiometric probe

5 mg

PK-CA707-61019

TMRE

Tetramethylrhodamine ethyl ester, perchlorate;


membrane potential sensitive dye

25 mg

PK-CA707-70016

TMRM

Tetramethylrhodamine methyl ester, perchlorate;


membrane potential sensitive dye

25 mg

PK-CA707-70017

Cytosolic Tracers and Related Reagents


Biocytin (e-biotinoylL-lysine)

Can be introduced into cells by microinjection

100 mg

PK-CA707-90055

Biocytin-Hydrazide

Can be introduced into cells by microinjection;


aldehyde-fixable

25 mg

PK-CA707-90060

Biotin-4-Fluorescein

Can be used as polar tracer to study cell morphology

10 mg

PK-CA707-90062

Biotin-Ethylenediamine,
hydrobromide

Equivalent to Neurobiotin; transneuronal tracer

25 mg

PK-CA707-90057

Biotin-Ethylenediamine,
hydrochloride

Functionally equivalent to Neurobiotin;


transneuronal tracer

25 mg

PK-CA707-90075

Biotin-Rhodamine 110

Can be used as polar tracer to study cell morphology

5 mg

PK-CA707-80022

Cell Analysis

https://www.promokine.info/shop

114

6 Cell Analysis

Cell Analysis

https://www.promokine.info/shop
Product

Product Description

Size

Catalog Number

Calcein AM

Viable cell stain (cytosol); acetoxymethyl ester

1 mg
20 x 50 g

PK-CA707-80011
PK-CA707-80011-3

Calcein AM Solution,
1 mg/ml in anhydrous DMSO

Viable cell stain (cytosol); acetoxymethyl ester

1 ml

PK-CA707-80011-2

Calcein AM Solution,
4 mM DMSO solution

Viable cell stain (cytosol); acetoxymethyl ester

100 l

PK-CA707-80011-1

Calcein, high purity

Viable cell stain (cytosol); ultra-pure

100 mg

PK-CA707-80013

DPA/Terbium for
membrane fusion assay

Can be used for vesicle fusion assays

1 set

PK-CA707-80104

DPX

Can be used to study membrane fusion or permeability

500 mg

PK-CA707-80012

Fluorescein-Biotin

5-((N-(5-(N-(6-(biotinoyl)amino)hexanoyl)amino)pentyl)
thioureidyl) fluorescein

5 mg

PK-CA707-80019

Hydroxystilbamidine

Also known as FluoroGoldTM

10 mg

PK-CA707-80014

Hydroxystilbamidine

TM

4% in H2O; also known as FluoroGold

200 l

PK-CA707-80023

Lucifer Yellow Cadaverine

Can be used as fixable fluorescent tracer

25 mg

PK-CA707-80018

Lucifer Yellow Cadaverine


Biotin-X, dipotassium salt

Useful tracer for studying neuronal morphology

10 mg

PK-CA707-80017

Lucifer Yellow CH lithium salt

Useful polar tracer for studying neuronal morphology

25 mg

PK-CA707-80015

Lucifer Yellow CH potassium


salt

Useful polar tracer for studying neuronal morphology

25 mg

PK-CA707-80016

RuBP-2S

Ruthenium(bis(bathophenanthroline))(bathophenanthrolinedisulfonate

5 mg

PK-CA707-80024

RuBP-4S

Ruthenium(bathophenanthroline)(bis(bathophenanthroli
nedisulfonate)), disodium salt

5 mg

PK-CA707-80025

RuBPS

Ruthenium(tris(bathophenanthrolinedisulfonate),
tetrasodium salt

5 mg

PK-CA707-80021

SDIP/Europium
for membrane fusion assay

Can be used for vesicle fusion assays

1 set

PK-CA707-80105

Sulforhodamine 101

Useful polar tracer for studying neuronal morphology


and cell-cell communication

500 mg

PK-CA707-80101

Sulforhodamine B

Useful polar tracer for studying neuronal morphology


and cell-cell communication

5g

PK-CA707-80100

Sulforhodamine G

Useful polar tracer for studying neuronal morphology


and cell-cell communication

5g

PK-CA707-80102

10 ml

PK-CA707-90082

Related Products
DMSO, anhydrous

Dimethylsulfoxide, anhydrous

Pluronic F-127

Nonionic detergent useful for solubilizing large dye


molecules such as Calcein/AM, Fura-2/AM, Fluo-3/AM,
Indo-1/AM and Rhod-2/AM to facilitate cell loading

1g

PK-CA707-59000

Pluronic F-127,
20% solution in DMSO

Nonionic detergent useful for solubilizing large dye


molecules such as Calcein/AM, Fura-2/AM, Fluo-3/AM,
Indo-1/AM and Rhod-2/AM to facilitate cell loading

1 ml

PK-CA707-59004

Pluronic F-127,
10% in H2O

Nonionic detergent useful for solubilizing large dye


molecules such as Calcein/AM, Fura-2/AM, Fluo-3/AM,
Indo-1/AM and Rhod-2/AM to facilitate cell loading

30 ml

PK-CA707-59005

Note: Please check our website regularly for new cell stains.

6 Cell Analysis

115

6.7 Intracellular Indicators and Related Products

6.7.1 Calcium Indicators


Applications



Intracellular calcium signaling studies


Investigation of signal transduction
and apoptotic cascades
Neuroscience research

Features
High quality and sensitivity

Derivatives for special applications


available
Compatible with standard fluores cence equipment
Calcium ions are important second messengers in cells. They are involved in
muscle contraction, nerve signal transmission, immune cell activation, hormone secretion, etc.
Fluorescent calcium indicators selectively
bind to calcium ions and show a spectral response upon binding Ca2+, i.e.,
fluorescence wavelength or intensity are
altered. This shift of fluorescence wavelength and change of intensity upon calcium chelation can be monitored using
fluorescence microscopy.
PromoKine offers a wide range of fluorescent calcium indicators for detecting
changes in the intracellular Ca2+ concentration, including Fura-2, Fluo-3, Rhod2, and Indo-1 (as cell-impermeable salts
or cell-permeable AM esters) as well as
their low-affinity derivatives Fura-2FF,

n Calcium Indicators
Product

Fluo-3FF, and Indo-1FF which are particularly useful for certain applications
(e.g., spatial and functional characterization of Ca2+ stores or detection of
NMDA-induced neuronal Ca2+ fluxes).
PromoKine also provides a range of nonfluorescent calcium chelators as well as
coelenterazine derivatives which are effective luminescent calcium indicators.

6.7.2 Other Indicators


Applications
pH determination
Iron, zinc, and chloride ion detection

Features
High quality and sensitivity
In addition to our calcium probes, PromoKine also offers pH indicators as well
as indicators for iron, zinc, and chloride
ion detection.

https://www.promokine.info/shop
Product Description

Size

Catalog Number

Fluorescent Calcium Indicators


Fluo-3 (pentaammonium salt)

Fluorescent calcium indicator; Ex/Em: 490-506/526

1 mg

PK-CA707-50010

Fluo-3 (pentapotassium salt)

Fluorescent calcium indicator; Ex/Em: 490-506/526

1 mg

PK-CA707-50011

Fluo-3 (pentasodium salt)

Fluorescent calcium indicator; Ex/Em: 490-506/526

1 mg

PK-CA707-50012

Fluo-3 AM

Fluorescent calcium indicator (acetoxymethyl ester)

10 x 100 g
20 x 50 g
1 mg

PK-CA707-50013
PK-CA707-50016
PK-CA707-50014

Fluo-3 AM Solution

Fluorescent calcium indicator (acetoxymethyl ester; 1 mM


in anhydrous DMSO)

1 ml

PK-CA707-50015

Fura-2 (pentaammonium salt)

Fluorescent calcium indicator; Ex/Em: 340-380/510

1 mg

PK-CA707-50030

Fura-2 (pentapotassium salt)

Fluorescent calcium indicator; Ex/Em: 340-380/510

1 mg

PK-CA707-50031

Fura-2 (pentasodium salt)

Fluorescent calcium indicator; Ex/Em: 340-380/510

1 mg

PK-CA707-50032

Fura-2 AM

Fluorescent calcium indicator (acetoxymethyl ester)

10 x 100 g
20 x 50 g
1 mg

PK-CA707-50033
PK-CA707-50033-1
PK-CA707-50034

Fura-2 AM Solution

Fluorescent calcium indicator (acetoxymethyl ester; 1 mM


in anhydrous DMSO)

1.0 ml

PK-CA707-50029

Indo-1 (pentaammonium salt)

Fluorescent calcium indicator; Ex/Em: 330/405-485

1 mg

PK-CA707-50040

Indo-1 (pentapotassium salt)

Fluorescent calcium indicator; Ex/Em: 330/405-485

1 mg

PK-CA707-50041

Indo-1 (pentasodium salt)

Fluorescent calcium indicator; Ex/Em: 330/405-485

1 mg

PK-CA707-50042

Indo-1 AM

Fluorescent calcium indicator (acetoxymethyl ester)

10 x 100 g
20 x 50 g
1 mg

PK-CA707-50043
PK-CA707-50043-1
PK-CA707-50044

Rhod-2 (triammonium salt)

Fluorescent calcium indicator; Ex/Em: 533-556/~578

1 mg

PK-CA707-50020

Cell Analysis

Signal transduction often starts with the


activation of a receptor on the cell surface which subsequently triggers a cascade of second messengers in the cell.
The change of intracellular ion concentration is a crucial step that helps second
messengers transmit information. Consequently, monitoring and control of intracellular ion concentration is a prerequisite understanding second messengers
and signal transduction in living cells.

116

6 Cell Analysis

https://www.promokine.info/shop
Product

Product Description

Size

Catalog Number

Rhod-2 (tripotassium salt)


Rhod-2 (trisodium salt)

Fluorescent calcium indicator; Ex/Em: 533-556/~578

1 mg

PK-CA707-50021

Fluorescent calcium indicator; Ex/Em: 533-556/~578

1 mg

PK-CA707-50022

Rhod-2 AM

Fluorescent calcium indicator (acetoxymethyl ester)

10 x 100 g
1 mg

PK-CA707-50023
PK-CA707-50024

Cell Analysis

Fluorescent High Calcium Indicators


5,5'-dibromo-Bapta
(tetrapotassium salt)

Fluorescent calcium indicator

100 mg

PK-CA707-50004

5,5'-difluoro-Bapta
(tetrapotassium salt)

Fluorescent calcium indicator

100 mg

PK-CA707-50006

5,5'-difluoro-Bapta AM

Fluorescent calcium indicator (acetoxymethyl ester)

25 mg

PK-CA707-50005

1 mg

PK-CA707-50045

500 g

PK-CA578-F1046

Bis-Fura-2, hexapotassium salt

Fluorescent calcium indicator

Fura-2FF (K+ Salt)

Difluorinated derivative of Fura-2 (K+ Salt)

Fura-2FF AM

Difluorinated derivative of Fura-3 (acetoxymethyl ester)

500 g

PK-CA578-F1044

Furaptra, AM ester

Fluorescent calcium indicator (also known as Mag-Fura-2)

10 x 100 g
20 x 50 g
1 mg

PK-CA707-50037
PK-CA707-50039
PK-CA707-50038

Furaptra, tetrapotassium salt

Fluorescent calcium indicator (also known as Mag-Fura-2)

1 mg

PK-CA707-50035

Furaptra, tetrasodium salt

Fluorescent calcium indicator (also known as Mag-Fura-2)

1 mg

PK-CA707-50036

Indo-1FF (K Salt)

Difluorinated derivative of Indo-1 (K Salt)

500 g

PK-CA578-I1086

Indo-1FF AM

Difluorinated derivative of Indo-1 (acetoxymethyl ester)

500 g

PK-CA578-I1084

Fluorescent Near-Membrane Calcium Indicators


FFP-18 AM

Fluorescent near-membrane calcium indicator


(Fura-derivative; acetoxymethyl ester)

1 mg

PK-CA578-F1052-1

FIP-18 AM

Fluorescent near-membrane calcium indicator


(Indo-derivative; acetoxymethyl ester)

500 ug

PK-CA578-F1054

5,5'-dibromo-Bapta
(tetrapotassium salt)

Fluorescent calcium indicator

100 mg

PK-CA707-50004

5,5'-dimethyl BAPTA, AM
ester

Fluorescent calcium indicator (acetoxymethyl ester)

25 mg

PK-CA707-50007

5,5'-dimethyl BAPTA,
tetrapotassium salt

Fluorescent calcium indicator

100 mg

PK-CA707-50008

Bapta (tetracesium salt)

Non-fluorescent calcium chelator

1g

PK-CA707-50001

Bapta (tetrapotassium salt)

Non-fluorescent calcium chelator

1g

PK-CA707-50002

Bapta (tetrasodium salt)

Non-fluorescent calcium chelator

1g

PK-CA707-50003

Bapta AM

Non-fluorescent calcium chelator (acetoxymethyl ester)

25 mg
20 x 1 mg

PK-CA707-50000
PK-CA707-50000-1

Bapta-FF (free acid)

Difluorinated derivative of BAPTA

10 mg

PK-CA578-B1091

Bapta-FF AM

Difluorinated derivative of BAPTA (acetoxymethyl ester)

10 mg

PK-CA578-B1093

Coelenterazine (native)

Coelenterazine; luminescent calcium indicator


& luciferase substrate

50 g

PK-CA707-10110

Coelenterazine 400a

Coelenterazine; luminescent calcium indicator


& luciferase substrate (also known as DeepBlue C,
a trademark of Perkin Elmer)

50 g

PK-CA707-10125

Coelenterazine cp

Coelenterazine derivative; luminescent calcium indicator


& luciferase substrate

50 g

PK-CA707-10112

Coelenterazine f

Coelenterazine derivative; luminescent calcium indicator


& luciferase substrate

50 g

PK-CA707-10114

Coelenterazine fcp

Coelenterazine derivative; luminescent calcium indicator


& luciferase substrate

50 g

PK-CA707-10117

Calcium Chelators

Luminescent Calcium Indicators

6 Cell Analysis

117

Product

Product Description

Size

Catalog Number

Coelenterazine h

Coelenterazine derivative; luminescent calcium indicator


& luciferase substrate

50 g

PK-CA707-10111

Coelenterazine hcp

Coelenterazine derivative; luminescent calcium indicator


& luciferase substrate

50 g

PK-CA707-10113

Coelenterazine i

Coelenterazine derivative; luminescent calcium indicator


& luciferase substrate

50 g

PK-CA707-10121

Coelenterazine ip

Coelenterazine derivative; luminescent calcium indicator


& luciferase substrate

50 g

PK-CA707-10116

Coelenterazine n

Coelenterazine derivative; luminescent calcium indicator


& luciferase substrate

50 g

PK-CA707-10115

Coelenterazine, 2-methyl

Coelenterazine derivative; luminescent calcium indicator


& luciferase substrate

50 g

PK-CA707-10122

Coelenterazine Sampler Kit

Coelenterazine and derivatives thereof

1 Kit

PK-CA707-10123

n Other Indicators
Product

https://www.promokine.info/shop
Product Description

Size

Catalog Number

Zn Indicators
Zinquin, ethyl ester

Blue-fluorescent Zn indicator

5 mg

PK-CA707-52020

Zinquin, free acid

Blue-fluorescent Zn indicator

5 mg

PK-CA707-52022

100 mg

PK-CA707-52011

Cl Indicators
MQAE

N-(ethoxycarbonylmethyl)-6-methoxyquinolinium
bromide; fluorescent chloride ion indicator

SPQ

6-methoxy-N-(3-sulfopropyl)quinolinium;
fluorescent chloride ion indicator

50 mg

PK-CA707-52010

Tetrakis-(2-pyridylmethyl)ethylenediamine;
heavy metal chelator

100 mg

PK-CA707-59003

5(6)-CFDA

5-(and-6)-carboxyfluorescein diacetate;
fluorescent pH indicator

100 mg

PK-CA707-51014

5(6)-CFDA, SE (CFSE)

5-(and-6)-carboxyfluorescein diacetate,
succinimidyl ester

25 mg

PK-CA707-90041

5-(and-6)-Carboxy-2',7'dichlorofluorescein

Fluorescent pH indicator

200 mg

PK-CA707-51015

5-(and-6)-Carboxy-2',7'dichlorofluorescein diacetate

Fluorescent pH indicator; cell membrane-permeant

100 mg

PK-CA707-51016

5-(and-6)-Carboxy-2',7'dichlorofluorescein diacetate,
succinimidyl ester (CDFDA,
SE)

Fluorescent pH indicator; cell membrane-permeant

25 mg

PK-CA707-90040

5-(and-6)-Carboxyfluorescein

Fluorescent pH indicator

100 mg

PK-CA707-51013

5-Carboxy-2',7'dichlorofluorescein

Fluorescent pH indicator (single isomer)

100 mg

PK-CA707-51017

5-Carboxy-2',7'dichlorosulfonefluorescein

Fluorescent pH indicator (single isomer)

10 mg

PK-CA707-51023

5-CFDA

5-Carboxyfluorescein diacetate (single isomer);


fluorescent pH indicator

100 mg

PK-CA707-51018

5-FAM

5-Carboxyfluorescein; fluorescent pH indicator

100 mg

PK-CA707-51019

6-CFDA

6-Carboxyfluorescein diacetate (single isomer);


fluorescent pH indicator

100 mg

PK-CA707-51021

Heavy Metal Ion Indicators


TPEN
pH Indicators

Cell Analysis

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118

6 Cell Analysis

Cell Analysis

https://www.promokine.info/shop
Product

Product Description

Size

Catalog Number

6-FAM

6-carboxyfluorescein; fluorescent pH indicator

BCECF

2',7'-bis-(carboxyethyl)-5-(and-6)-carboxyfluorescein;
fluorescent pH indicator

100 mg

PK-CA707-51020

1 mg

PK-CA707-51010

BCECF AM

Fluorescent pH indicator; acetoxymethyl ester

10 x 100 g
20 x 50 g
1 mg

PK-CA707-51011
PK-CA707-51011-1
PK-CA707-51012

BCECF AM Solution

Fluorescent pH indicator; acetoxymethyl ester


(1 mg/ml in dry DMSO)

1 ml

PK-CA707-51009

Flubida-2

Fluorescent pH indicator

5 mg

PK-CA707-51022

n Related Products

https://www.promokine.info/shop

Product

Product Description

4-Bromo A-23187 free acid

For in situ calibration of fluorescent calcium indicators

A-23187 free acid

A-23187 Free Acid (calcimycin; or Calcium Ionophore III)

Calcium Calibration Buffer Kit

For calibration of fluorescent calcium indicators

DMSO, anhydrous

Dimethylsufoxide, anhydrous

EDC (EDAC)

1-(3-dimethylaminopropyl-3-ethylcarbodiimide,
hydrochloride)

Flubi-2

Calibration standard for Flubida-2

5 mg

PK-CA707-51024

Ionomycin (calcium salt)

Useful in cell activation experiments when calcium


dose-response data are not required (>90% pure)

5 mg

PK-CA577-1566-5

Ionomycin (free acid)

Useful for studies of Ca2+ transport across biological


membranes and measurement of cytoplasmic free Ca2+;
induces apoptotic neuronal degeneration in embryonic
cortical neurons (>90% pure)

5 mg

PK-CA577-1565-5

Pluronic F-127

Nonionic detergent useful for solubilizing large dye


molecules such as Fura-2/AM, Fluo-3/AM,
Indo-1/AM, and Rhod-2/AM to facilitate cell loading

1g

PK-CA707-59000

Pluronic F-127, 10% in H2O

Nonionic detergent useful for solubilizing large dye


molecules such as Fura-2/AM, Fluo-3/AM,
Indo-1/AM, and Rhod-2/AM to facilitate cell loading

30 ml

PK-CA707-59005

Pluronic F-127, 20% solution


in DMSO

Nonionic detergent useful for solubilizing large dye


molecules such as Fura-2/AM, Fluo-3/AM,
Indo-1/AM, and Rhod-2/AM to facilitate cell loading

1 ml

PK-CA707-59004

Note: Please check our website regularly for new intracellular indicators.

Size

Catalog Number

1 mg

PK-CA707-59006

1 mg
10 mg

PK-CA707-59001
PK-CA577-1501

2 x 50 ml

PK-CA707-59100

10 ml

PK-CA707-90082

100 mg

PK-CA707-59002

6 Cell Analysis

119

6.8 Cell Signaling Assays

Quantitative determination of
cAMP/cGMP levels
Cell signaling and cell growth/
differentiation studies
b-Secretase activity detection
Alzheimers disease drug development

Features
Fast and simple assays
Highly sensitive

cAMP and cGMP Immunoassay Kits


Adenosine 3,5-cyclic monophosphate
(cyclic AMP, cAMP) is one of the most
important second messengers involved
as a modulator in physiological processes. It regulates neuronal, glandular, and
cardiovascular processes as well as immune mechanisms, the nervous system,
cell growth, and cell differentiation. A
number of hormones (e.g. ACTH, TSH,
LH) are known to activate cAMP via the
action of the enzyme adenylate cyclase
which converts ATP to cAMP.

Guanosine 3,5-cyclic monophosphate


(cyclic GMP, cGMP) has been shown to
be present at levels typically 10-100 fold
lower than cAMP in most tissues and is
formed by the action of the enzyme guanylate cyclase on GTP. Some hormones
(e.g., acetylcholine, insulin, and oxytocin) and chemicals (e.g., serotonin and
histamine) cause an increase in cGMP
levels.
The PromoKine cAMP and cGMP competitive immunoassays were developed
for the quantitative determination of
cAMP and cGMP levels. They utilize
polyclonal capture antibodies against
cAMP or cGMP coated on a 96-well
plate and HRP-conjugated cAMP or
cGMP-HRP which competes with the
cAMP or cGMP from the sample. After
HRP-substrate addition, the amount of
cAMP-HRP or cGMP-HRP bound to the
plate can easily be determined spectrophotometrically at 450 nm. The intensity
of the blue color is inversely proportional
to the cAMP or cGMP concentration in
the sample. Due to a novel acetylation
procedure 1-100 fmol cAMP or 0.1-10

pmol cGMP can be detected. This is


approximately 10 times more sensitive
than similar kits.

b-Secretase Fluorometric Assay Kit


b-Secretase (BACE) is a membranebound aspartyl protease that cleaves
the amyloid precursor protein and is
consequently an excellent target for
anti-amyloid therapy in the treatment of Alzheimers disease. Finding
inhibitors of b-secretase is one of the
major goals of Alzheimers disease
drug development. The PromoKine
b-Secretase Fluorometric Assay provides
a convenient, non-radioactive system for
detecting b-secretase activity in biological samples. The kit provides active bsecretase as positive control, b-secretase
inhibitor as negative control, optimized
peptide substrate (conjugated to two
reporter molecules), and buffers for
convenient measurement of b-secretase
activity in mammalian samples.
b-Secretase inhibitors are also available
separately.

https://www.promokine.info/shop
Product

Size

Catalog Number

cAMP Immunoassay Kit

100 assays

PK-CA577-K371

cGMP Immunoassay Kit

100 assays

PK-CA577-K372

Beta-Secretase Fluorometric Assay Kit

100 assays

PK-CA577-K360

Beta-Secretase Inhibitor I

1 mg

PK-CA577-7501-1

Beta-Secretase Inhibitor II

1 mg

PK-CA577-7502-1

Note: Please check our website regularly for new cell-based assays.

Cell Analysis

Applications

120

6 Cell Analysis

6.9 Cell Stress and Metabolism Kits

Cell Analysis

Applications
GST activity measurement and quanti-
tation of GST-tagged fusion proteins
SOD assays
Determination of the concentration of
NO metabolites (nitrate/nitrite)
HAT activity measurement
Metabolic studies and analysis of
mutagenic, carcinogenic, and toxic
effects
DNA damage detection and
quantification
Analysis of the cell redox state

CDNB is totally dependent on the presence of active GST. The detection limit
is approximately 10 ng active GST/assay.
The free form of MCB is almost nonfluorescent, whereas the dye fluoresces
blue (Ex/Em = 380/461 nm) when reacting with glutathione. GST catalyzes the
MCB-glutathione reaction and the fluorescence levels are proportional to the
amount of GST present in the reaction.
Therefore, the GST level in samples can
be easily detected using a fluorometer or
a 96-well fluorometric plate reader.

fluorometric reagent DAN followed by


NaOH which converts nitrite into a fluorescent compound (Fluorometric Nitric
Oxide Kits) or the addition of Griess Reagent which converts nitrite into a deep
purple azo compound (Colorimetric
Nitric Oxide Kits). Measurement of the
fluorescent compound reaction product
or azo chromophore accurately determines the total nitric oxide production.
The Fluorometric Assay Kit has been
validated with culture media, plasma,
and tissue homogenates.

SOD Assay Kit

HAT Activity Assay Kit

Superoxide dismutase (SOD) is one of


the most important antioxidative enzymes. The sensitive SOD Assay Kit
utilizes WST-1 that produces a watersoluble formazan dye upon reduction
with the superoxide anion. The rate of
the reduction with a superoxide anion is
linearly related to the xanthine oxidase
(XOD) activity and is inhibited by SOD
(Fig. 8). Therefore, the inhibition activity
of SOD can be determined by a colorimetric method.

Histone acetyltransferases (HATs) have


been implicated in playing a crucial role
in various cellular functions such as gene
transcription, cell differentiation, and
proliferation.
The colorimetric HAT Activity Assay
Kit offers a convenient non-radioactive
system for rapid and sensitive spectrophotometric detection of HAT activity in
mammalian samples. The detection can
be continuous and is suitable for kinetic
studies.

Fluorometric and Colorimetric


Nitric Oxide Kits

DNA Damage Detection Kit

Features
Highly sensitive and reproducible
detection/measurement
Ready-to-use, convenient assays

Fluorometric and Colorimetric GST


Assay Kits
Glutathione (GSH) is the major intracellular low-molecular-weight thiol and plays
a critical role in cellular defense against
oxidative stress in tissues and cells. Glutathione S-transferase (GST) is involved
in detoxification of xenobiotics mediating
conjugation of glutathione with those
compounds.
The Fluorometric and Colorimetric GST
Assays can detect GST activity in crude
cell lysates or purified protein fractions
and the kits also quantitate GST-tagged
fusion proteins. They are based upon the
GST-catalyzed reaction between GSH
and the GST substrate CDNB (Colorimetric Kit) or MCB (Fluorometric Kit).
CDNB has the broadest range of isozyme
detectability (a-, -, f-, and other GST
isoforms). Under certain conditions, the
interaction between glutathione and

Nitric oxide (NO) plays an important role


in neurotransmission, vascular regulation, immune responses, and apoptosis.
The Fluorometric and Colorimetric Nitric
Oxide Assay Kits provide an accurate and
convenient tool for total nitrate/nitrite
concentration determination in a simple
two-step process that can be used as a
quantitative measure of NO production.
The first step is the conversion of nitrate
to nitrite utilizing nitrate reductase. The
second step involves the addition of the

Xanthine
O2

2O2

SO3

O3S

I
N N
H

NO2

Xanthine oxidase

Uric acid
H2O2

WST-1 Formazan

N N

-.

2O2

SO3

O3S

I
N

+
N N

Na +
NO2

SOD

O2 + H2O2

Inhibition reaction

Fig. 8: Principle of the SOD Assay Kit.

WST-1

Different physiological and environmental factors such as chemically induced


damage and cell aging cause DNA lesions. AP sites (apurinic/apyrimidinic
sites) are one of the major types of
DNA lesions formed during the process
of base excision and repair of oxidized,
deaminated, or alkylated bases. It has
been estimated that about 2x105 base
lesions are generated per cell per day.
For this reason, the level of AP sites in
cells can be a good indicator of DNA lesions and repairs. The PromoKine DNA
Damage Detection Kit utilizes the ARP
(Aldehyde Reactive Probe) reagent that
reacts specifically with the aldehyde
group in the open ring form of the AP
sites. After treating DNA containing AP
sites with ARP reagents, the AP sites are
tagged with biotin residues, which can
be quantified using an avidin-biotin assay followed by colorimetric detection.
The kit provides the necessary reagents
for convenient determination of abasic
sites in purified DNA samples in a 96-well
plate format.

NAD/NADH and NADP/NADPH


Quantitation
PromoKines NAD/NADH and NADP/
NADPH Quantitation Kits provide a convenient tool for sensitive detection of the
intracellular nucleotides NAD, NADH,
NADP, and NADPH along with the determination of their ratios. They can be
used for studies of energy transformation and the determination of the redox
state of cells or tissue.
The kits have been designed to specifically recognize NAD/NADH only or NADP/
NADPH only in an enzyme cycling reaction that significantly increases detection
sensitivity. Purification of NAD/NADH or
NADP/NADPH from sample mixes is not
required.

Fluorometric & Colorimetric ATP


Quantitation Kit
ATP that is formed exclusively in the mitochondria is the major energy currency
of living systems and virtually all energy
requiring processes utilize the chemical
energy stored in the phosphate bond

of ATP. A variety of genetic diseases


can affect ATP formation in the mitochondria. There are a number of commercially available ATP assays available
that can detect very low amounts of ATP
by measuring luminescence (PromoKine
Kit PK-CA577-254-200, for example).
However, these kits require specialized
luminescence instrumentation and utilize
luciferase which can be difficult to maintain in an active form.
PromoKines new Colorimetric & Fluorometric ATP Quantitation Kit is designed
to be robust and has a simple straightforward method which utilizes the phosphorylation of glycerol to generate a
product that is easily quantified by colorimetric (max = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The
assay can detect as low as 50 picomole
(1 M) of ATP in various samples.

Fluorometric & Colorimetric ADP


Quantitation Kit
ADP is a product of ATP dephosphorylation and can be rephosphorylated back
to ATP. Dephosphorylation and rephos-

121

phorylation occur via various phosphatases, phosphorylases and kinases. ADP


is stored in platelets and can be released
to interact with a variety of purinergic
receptors. ADP levels regulate several
enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and
chloroplast, although several such processes occur in the cytoplasm. Conventionally, ADP levels have been measured
by luciferase/luciferin mediated assays
after ADP is converted to ATP. However,
the luciferase system is unstable and luminescence equipment is not generally
available in most laboratories.
PromoKines new Fluorometric & Colorimetric ADP Quantitation Kit provides a
convenient colorimetric and fluorometric
method to measure the ADP level. In this
assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be
quantified colorimetrically (max = 570
nm) or fluorometrically (Ex/Em 535/587
nm). The assay is simple, sensitive, stable, and high-throughput adaptable. It
can detect as low as 1 M ADP in biological samples.

https://www.promokine.info/shop
Product
Colorimetric GST Assay Kit

Size

Catalog Number

100 assays

PK-CA577-K263

Fluorometric GST Assay Kit

100 assays

PK-CA577-K260

HAT Activity Assay Kit

100 assays

PK-CA577-K332

Colorimetric Nitric Oxide Assay Kit

2 x 96 assays

PK-CA577-K262

Fluorometric Nitric Oxide Assay Kit

2 x 96 assays

PK-CA577-K252

SOD Assay Kit


DNA Damage Detection Kit

100 assays

PK-CA577-K335

25 assays

PK-CA577-K253

NAD/NADH Quantitation Kit

100 assays

PK-CA577-K337

NADP/NADPH Quantitation Kit

100 assays

PK-CA577-K347

Fluorometric & Colorimetric ATP


Quantitation Kit

100 assays

PK-CA577-K354

Fluorometric & Colorimetric ADP


Quantitation Kit

100 assays

PK-CA577-K355

100 g

PK-RP577-1137-100

1g

PK-CA577-1556-1

HAT protein (P/CAF, active)


GST Inhibitor-2 (ethacrynic acid)
Oxidized Glutathione (GSSG)

1g

PK-CA577-1241-1

Reduced Glutathione (GSSG)

1g

PK-CA577-1242-1

GST protein (active)


Superoxide Dismutase (SOD, human)

Note: Please check our website regularly for new cell-based assays.

1 mg

PK-RP577-1243-1

100 g

PK-RP577-4802-100

Cell Analysis

6 Cell Analysis

122

6 Cell Analysis

6.10 Cell Fractionation and Protein Extraction Kits


Applications

Membrane Protein Extraction Kit

The Membrane Protein Extraction Kit


provides an easy and complete protocol
for isolating integral membrane proteins
efficiently from cultured mammalian
cells. The kit was designed to not only
extract the total cellular membrane proteins but also to specifically purify the
plasma membrane proteins. The procedure offers consistent yield and high
purity (>90%). Membrane proteins prepared using the kit can be utilized in a
variety of applications such as Western
blotting, 2-D gels, enzyme analyses,
etc. The entire procedure takes less than
1 hour.

Cellular protein extraction from


mammalian cells
Integral membrane protein extraction
Preparation of highly enriched cellular
fractions (mitochondria, cytosol,
nucleus) from mammalian cells

Cell Analysis

Features




Convenient and efficient cell fractionation and protein extraction


Easy protocols
Proteins and cell fractions can be used
for many downstream applications

Mammalian Whole Cell Protein


Extraction Kit
The Mammalian Whole Cell Protein
Extraction Kit was optimized for complete extraction of all cellular proteins.
It contains an optimized lysis buffer and
a protease inhibitor cocktail as well as
other reagents for convenient extraction
of mammalian proteins from tissues and
cells under non-denaturing conditions.
The entire procedure takes less than 20
minutes and the proteins can be used for
many downstream applications such as
gel electrophoresis, Western blotting, etc.

Mitochondria/Cytosol
Fractionation Kit

Nuclear/Cytosol Fractionation Kit


The Nuclear/Cytosol Fractionation Kit
provides a complete system that enables the separation of nuclear and
cytoplasmic fractions of mammalian
cells and tissues with little or no crosscontamination, maintaining the nuclear
and cytoplasmic compartments separate
and intact.

The Mitochondria/Cytosol Fractionation


Kit allows isolation of a highly enriched
mitochondrial fraction from the cytosol of mammalian cells in a simple and
staightforward procedure without ultracentrifugation and toxic chemicals.

Cytosol/Particulate Fractionation Kit


The Cytosol/Particulate Fractionation
Kit provides an easy and convenient system for rapid separation of cytosol and
particulate fractions. The separated fractions are compatible with downstream
applications such as assaying cellular
distributions of small molecules and proteins or examining signal transduction
pathways.

Complete Cell Fractionation Kit


The Complete Cell Fractionation Kit is
designed for serial sample preparation of
four distinct protein fractions, including
cytosol, particulate, cytoskeleton, and
nuclear fractions from one sample.
The prepared fractions are suitable for
many downstream assays such as twodimensional gel electrophoresis, protein
immunoblotting, gel-shift assays, enzyme activity assays, reporter assays,
and more.

https://www.promokine.info/shop
Product
Mammalian Whole Cell Protein Extraction
Kit

Size

Catalog Number

500 assays

PK-CA577-K269

Membrane Protein Extraction Kit

50 assays

PK-CA577-K268

Nuclear/Cytosol Fractionation Kit

25 assays
100 assays

PK-CA577-K266-25
PK-CA577-K266-100

Mitochondria/Cytosol Fractionation Kit

25 assays
100 assays

PK-CA577-K256-25
PK-CA577-K256-100

Cytosol/Particulate Fractionation Kit

50 assays

PK-CA577-K267

Complete Cell Fractionation Kit

50 assays

PK-CA577-K270

6 Cell Analysis

123

6.11 Protein Size Markers and Protein Gel Stains

FluoProtein Gel Stain is a fast and sensitive fluorescent dye for visualization and
quantitation of proteins separated by
1-D or 2-D SDS-PAGE and an excellent,
economic alternative to common fluorescent gel stains. FluoProtein Gel Stain
normally emits a low fluorescence, but is
strongly fluorescent (bright golden color)
when bound to proteins. The maximum
emission wavelength of protein-bound
FluoProtein Gel Stain is near 570 nm.
FluoProtein Gel Stain gives exceptional
sensitivity and a wide dynamic range for
protein detection.
FluoProtein Gel Stain comes as a 100x
stock solution that is simply diluted with
water by the user to its working concentration. The staining procedure is a simple
two-step protocol (fix and stain) that can
be completed in only 30 minutes. Gels
are fixed with ethanol/acetic acid solution prior to staining with FluoProtein Gel
Stain solution. A destain step is normally
not required. Gels stained with FluoProtein Gel Stain may be directly visualized
with a variety of different UV-based fluorescence imaging systems. The bound
FluoProtein Gel Stain is easily removed
from the protein by immersing the gel
in sufficient water, thus it is compatible
with subsequent enzymatic digestion
and mass spectrometry for proteomic applications.

GelQuick Protein Gel Stain/


Destaining Kit
Traditional staining and destaining
methods for SDS-PAGE protein gels

are known to take several hours and


also leave a high blue background
which reduces the sensitivity of band
detection.

ing a broad range (10-245 kDa) with


ten blue-stained protein bands and
two reference bands at 25 kDa (green)
and 75 kDa (red).

PromoKines GelQuick Gel Stain/Destaining Kit provides a fast and sensitive


method for SDS-PAGE gel staining and
destaining. The procedure only takes 5
minutes for gel staining and 15 minutes
for destaining.
After a total of 20 minutes, visualization
of as little as 5 ng of protein on a waterclear background can be seen.
The kit contains sufficient solutions to
stain and destain up to 40 protein mini
gels.

Cell Analysis

FluoProtein Gel Stain

Prestained RTU Protein Ladders


PromoKines Prestained RTU Protein
Ladders are very cost-effective and
supplied ready-to-use for direct loading onto gels. They can be used for
monitoring protein separation during
SDS-polyacrylamide gel electrophoresis,
estimation of protein sizes, and verification of Western transfer efficiency onto
membranes (PVDF, nylon, or nitrocellulose). They show excellent stability with
sharp bands and allow easy identification as bands of different colors are used.
Each Prestained RTU Protein Ladder is
sufficient for 100-200 mini gels.
The Prestained RTU Protein Ladder I
contains 9 pink-stained protein markers
covering the range from 10 to 175 kDa
and three blue-stained reference bands
at 10, 40 and 90 kDa.
The Prestained RTU Protein Ladder II
is a three-color protein standard cover-

Fig. 9: PromoKines RTU Protein Ladder


I (above) and RTU Protein Ladder II (below) separated on different SDS polyacrylamide gels.

https://www.promokine.info/shop
Product

Size

Catalog Number

1 ml
10 ml

PK-MB713-004-1
PK-MB713-004-10

For 40 minigels

PK-MB577-K901

Prestained RTU Protein Ladder I

2 x 0.25 ml

PK-MB713-001P

Prestained RTU Protein Ladder II

0.5 ml

PK-MB713-002P

FluoProtein Gel Stain


GelQuick Protein Gel Stain/Destaining Kit

124

6 Cell Analysis

6.12 Nucleic Acid Isolation Kits


Applications

Mitochondrial DNA Isolation Kit

DNA isolation

Fast, simple, and reproducible


procedures
Optimal yields
Highly pure DNA suitable for various
molecular genetic applications

The Mitochondrial DNA Extraction Kit


provides a convenient tool for isolating
mtDNA from a variety of cells and tissues with high yield and purity, without
contamination from genomic DNA. The
purified mtDNA can be used for a variety
of studies such as enzyme manipulation,
Southern blotting, cloning, PCR analysis,
and amplifications.

Genomic DNA Isolation Kit I

PCR Purification Kit

The Genomic DNA Isolation Kit I provides a simple and convenient procedure
for rapid isolation of genomic DNA from
mammalian cells and tissue samples with
high yield and purity. The novel method
requires less than 90 minutes to prepare
highly pure genomic DNA that is free
of protein and RNA and is suitable for
a variety of applications such as PCR,
DNA hybridization, cloning, Southern
blotting, and array-based experiments.

The PromoKine PCR Purification Kit has


been designed for the work-up of PCR
reactions (removal of primer dimers,
primers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities). The preparation is based on a
silica-membrane technology for binding
DNA in high-salt and elution in low-salt
buffer. The kit provides a simple and
efficient way to purify linear or circular
DNA and is optimized for working with

Cell Analysis

Features

DNA amounts of up to 50 g. It requires


no organic extractions or precipitation
and guarantees high and stable recovery
rates.

https://www.promokine.info/shop
Product
Genomic DNA Isolation Kit I
Mitochondrial DNA Isolation Kit
PCR Purification Kit

Size

Catalog Number

50 assays

PK-MB577-K281

50 isolations

PK-CA577-K280

50 preparations
250 preparations

PK-MB20-300-50
PK-MB20-300-250

6 Cell Analysis

125

6.13 DNA Size Markers and DNA Gel Stains


RTU DNA Ladders

PromoKines FluoDNA Gel Stain is a nonmutagenic fluorescent reagent that produces instant visualization of DNA bands
upon Blue Light or UV illumination of
agarose gels.
Supplied in 6x DNA Loading Buffer, it
is used to prepare DNA samples and
markers for loading onto agarose or
polyacrylamide gels. FluoDNA Gel Stain
is a highly sensitive stain available for detecting double-stranded DNA (dsDNA)
and contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for monitoring the DNA migration during the electrophoresis process.
FluoDNA Gel Stain is an economic, safe,
and non-hazardous alternative to ethidum bromide. It provides high sensitivity,
shows a low background, and causes less
DNA damage. It can therefore improve
efficiency in downstream applications
such as gene cloning.

PromoKines RTU DNA Ladders are suitable for use as molecular weight standards for agarose gel electrophoresis.
They are very cost-effective and supplied
ready-to-use, sufficient for 100-200 mini
gels.
The RTU DNA Ladder I displays 11 sharp
bands ranging from 100 to 1,500 base
pairs. Two bands (100 and 1,500 base
pairs) have an increased intensity and
serve as reference points. The ladder
comes ready-to-use containing Orange G
and Xylene Cyanol FF as tracking dyes.
The RTU DNA Ladder II includes 13 fragments ranging from 250 to 10,000 bp
with two bands (1,000 and 3,000 base
pairs) which have an increased intensity to serve as reference points. It comes
ready-to-use containing Bromphenol
Blue as tracking dye.

Base
Pairs

DNA Mass
(ng/5ml)

Base
Pairs

Cell Analysis

DNA Mass
(ng/5ml)

FluoDNA Gel Stain

Fig. 10: PromoKines RTU DNA


Ladder I (left) and RTU DNA Ladder II
(right) separated on 1.7% and 1.0%
TAE agarose gels, respectively.

https://www.promokine.info/shop
Product

Size

Catalog Number

RTU DNA Ladder I (100 bp)

0.5 ml

PK-MB713-001N

RTU DNA Ladder II (1000 bp)

0.5 ml

PK-MB713-002N

1 ml

PK-MB713-003

FluoDNA Gel Stain

Apoptosis
Apoptosis, or programmed cell death, is a sophisticated process
that involves an intricate series of biochemical events. Specific
cell death signals are transduced by different biomolecules such
as caspases, which initiate an apoptotic cascade. This process
culminates in multiple events such as degradation of cellular
proteins and chromosomal DNA, mitochondrial disruption, and
changes in plasma membrane integrity.

128 Content Chapter 7

3
7

Apoptosis
7.1 DNA Fragmentation Detection Kits . . . . . 129
7.2 Mitochondrial Apoptosis Staining Kit . . . . 130
7.3 Cytochrome c Apoptosis Detection Kit . . . 130
7.4 Glutathione Detection Kits . . . . . . . . . . . . 131
7.5 Annexin V Detection Kits . . . . . . . . . . . . . 132
7.6 Caspase Kits . . . . . . . . . . . . . . . . . . . . . . . 134
7.6.1

Caspase Detection Kits . . . . . . . . . . . . . . . . 134

7.6.2

Caspase Quantification Kits . . . . . . . . . . . . 136

7.7 Cathepsin/Calpain Detection Kits . . . . . . . 138


7.8

Kinase Detection Kits . . . . . . . . . . . . . . . . 139

7.9

Apoptotic and Necrotic Cell Detection . . . 140

7.10 Apoptotic Cell Isolation Kit . . . . . . . . . . . . 141


7.11 Apoptosis Inducers . . . . . . . . . . . . . . . . . . 141

7 Apoptosis

129

7.1 DNA Fragmentation Detection Kits

Detecting fragmented
nucleosomal DNA
Screening for apoptotic cells
Fluorescence microscopy
Flow cytometry
Western blotting

Features
High sensitivity
Rapid detection of apoptotic cells at
the single-cell level
Compatible with standard lab
equipment
Internucleosomal DNA fragmentation is
a key sign of apoptosis in mammalian
cells. PromoKine offers four different kits
for analyzing DNA fragmentation which
are based on fluorometric or colorimetric
assays. Since all of the kits are designed
for use with various multiwell formats,
they are also suited for automation.

Fluorometric DNA Fragmentation


Detection Kits I and II (BrdU)
The PromoKine DNA Fragmentation
Detection Kits I and II are based on the
TUNEL method and identify DNA strand
breaks that occur during apoptosis. The
kits provide all required components including positive and negative control
cells and tissue slides - for convenient
detection of DNA fragmentation by fluorescence microscopy or flow cytometry.
The TUNEL assay employs a terminal
deoxynucleotidyl transferase (TdT) to

add Br-dUTP (bromolated deoxyuridine


triphosphate nucleotides) to the fragmented DNA strands. Br-dUTP is more
readily incorporated into DNA strand
breaks than other larger ligands (e.g.,
fluorescein, biotin, or digoxigenin). The
incorporated Br-dUTP is subsequently
labeled with a green- (Kit I) or a redfluorescent (Kit II) anti-BrdU monoclonal
antibody resulting in a very bright signal.
The labeled DNA can then be observed
by fluorescence microscopy or analyzed
by flow cytometry. The DNA Fragmentation Kit II works also very well with GFP
transfected cells.

Fluorometric DNA Fragmentation


Detection Kit III (F-dUTP)
The Fluorometric DNA Fragmentation
Detection Kit III is a modified TUNEL assay. It provides all reagents required for
the specific detection of apoptotic cells in
a cell population, in tissue sections, and
in fixed cell preps. The kit utilizes terminal deoxynucleotidyl transferase (TdT) to
catalyze the incorporation of fluorescein12-dUTP at the free 3-hydroxyl ends of
the fragmented DNA. The fluoresceinlabeled DNA can then be observed by
fluorescence microscopy or analyzed by
flow cytometry.

Colorimetric DNA Fragmentation


Detection Kit (IHC)
The ready-to-use TUNEL-based Colorimetric DNA Fragmentation Detection
Kit (IHC) has been specifically designed
for expedient detection of DNA frag-

mentation in tissue sections and fixed


cell preparations. It includes positive
and negative control slides and utilizes
Br-dUTP, biotin-labeled anti-BrdU antibodies, and HRP-streptavidin conjugate.
Immunohistochemical staining results
can be analyzed with a microscope.

Apoptotic DNA Ladder


Detection Kit I
The Apoptotic DNA Ladder Detection
Kit I provides an easy and sensitive
method for detecting DNA fragmentation in apoptotic cells. DNA preparation
is done in less than 90 minutes without
the need for extraction or separation
columns. Afterwards, the DNA fragments can easily be visualized by agarose gel electrophoresis. This kit allows
the efficient recovery of small DNA fragments ensuring high sensitivity.
The Apoptotic DNA Ladder Detection
Kit IPlus additionally includes a highly
sensitive DNA staining dye for improved
visualization of DNA fragmentation by
agarose gel electrophoresis.

Apoptotic DNA Ladder


Detection Kit II
Using the Apoptotic DNA Ladder Extraction Kit II allows the selective isolation
of DNA ladders without interference of
regular genomic DNA. The kit can detect
apoptotic DNA ladders from tissues and
cells with an apoptotic cell population
of as little as 5%. The kit specifically extracts laddered DNA, not genomic DNA.

https://www.promokine.info/shop
Product

Size

Catalog Number

Fluorometric DNA Fragmentation Detection Kit I (BrdU)

60 assays

PK-CA577-K401

Fluorometric DNA Fragmentation Detection Kit II (BrdU)

60 assays

PK-CA577-K404

Fluorometric DNA Fragmentation Detection Kit III (F-dUTP)

50 assays

PK-CA577-K402

Colorimetric DNA Fragmentation Detection Kit (IHC)

50 assays

PK-CA577-K403

Apoptotic DNA Ladder Detection Kit I

50 assays

PK-CA577-K120

Apoptotic DNA Ladder Detection Kit I

50 assays

PK-CA577-K130

Apoptotic DNA Ladder Detection Kit II

50 assays

PK-CA577-K170

Plus

Note: Please check our website regularly for new cell-based apoptosis assays.

Apoptosis

Applications

130

7 Apoptosis

7.2 Mitochondrial Apoptosis Staining Kit


Applications
Screening for early apoptotic cells
Fluorescence microscopy
Flow cytometry

Apoptosis

Features






Excellent sensitivity due to high


fluorescence intensity
Simultaneous detection of apoptotic
and healthy cells
Excitation and emission spectra are
compatible with common excitation
sources and filter sets

Disruption of mitochondrial transmembrane potential is one of the earliest


intracellular events that occurs upon the
induction of apoptosis. The Mitochondrial Apoptosis Staining Kit provides a
simple, fluorescent-based in vitro assay
for detecting the mitochondrial changes
in apoptosis, and the kit distinguishes
between healthy and apoptotic cells.
The assay utilizes the novel cationic
dye MitoCaptureTM that fluoresces
differently in healthy versus apoptotic
cells. In healthy cells, MitoCaptureTM
accumulates and aggregates in the mitochondria - fluorescing brightly red. In

apoptotic cells, a shift in mitochondrial


transmembrane potential prevents the
dye from accumulating in the mitochondria. Instead, it remains in the cytoplasm
in its monomer form - fluorescing green.
The fluorescent signals can easily be
detected by fluorescence microscopy or
flow cytometry using common filters
and channels (FITC, rhodamine, PI). The
assay takes approximately 20 minutes, is
highly sensitive, and detects apoptosis
reliably in living cells.

https://www.promokine.info/shop
Product
Mitochondrial Apoptosis Staining Kit

Size

Catalog Number

25 assays
100 assays

PK-CA577-K250-25
PK-CA577-K250-100

7.3 Cytochrome c Apoptosis Detection Kit


Applications
Screening for apoptotic cells
Analysis of apoptotic signal cascades

Features
Non-radioactive method
Easy and fast procedure
No toxic chemicals necessary

Cytochrome c has been shown to play


an important role in apoptosis. The protein is located between the inner and
outer mitochondrial membranes. An
apoptotic stimulus triggers the release
of cytochrome c into the cytosol where
it binds to Apaf-1. The cytochrome
c/Apaf-1 complex activates caspase-9
which then activates caspase-3 and
other downstream caspases of the apoptotic cascade.
The PromoKine Cytochrome c Apoptosis
Detection Kit provides an effective means

for detecting cytochrome c translocation


from mitochondria into the cytosol during apoptosis. The kit is easy to use and
provides unique reagents for separating
a highly enriched mitochondria fraction
from the cytosol. Cytochrome c released
from mitochondria into the cytosol is
then detected by Western blotting using
the cytochrome c antibody provided in
the kit. The procedure is very simple and
straightforward. No ultracentrifugation
is required and no toxic chemicals are
involved.

https://www.promokine.info/shop
Product
Cytochrome c Apoptosis Detection Kit

Size

Catalog Number

100 assays

PK-CA577-K257

Note: Please check our website regularly for new cell-based apoptosis assays.

7 Apoptosis

131

7.4 Glutathione Detection Kits

Screening for apoptotic cells


Glutathione detection
Quantitation of glutathione
reductase activity

Features
Non-radioactive, highly sensitive
method
Easy and fast procedure
Compatible with standard lab
equipment

Fluorometric Glutathione
Detection Kit
Diminished glutathione (GSH) levels
occur at the early stages of apoptosis.
The Fluorometric Glutathione Detection
Kit provides a simple in vitro assay for
detecting the GSH changes in apoptosis
and other pathological processes. The
assay utilizes a dye (monochlorobimane,
MCB) that has a high affinity for glutathione. The unbound dye is almost nonfluorescent, whereas the dye fluoresces
blue (excitation=380 nm; emission=461
nm) when bound to reduced or oxidized
glutathione. Consequently, the decrease
in glutathione level in apoptotic cells can
be easily detected using a fluorometer or
a 96-well fluorometric plate reader.

Colorimetric Glutathione
Detection Kit
The Colorimetric Glutathione Detection
Kit provides a convenient colorimetric
method for analyzing either total glutathione or only the reduced form of glutathione using a microplate reader. The
assay utilizes the glutathione recycling
system with DTNB and glutathione reductase. GSH is regenerated from GSSG
(oxidized glutathione) by glutathione
reductase and reacts again with DTNB
to produce yellow 2-nitro-5-thiobenzoic
acid that can be detected spectrophotometrically at 412 nm (Fig. 1). This recycling reaction dramatically improves the
sensitivity of total glutathione detection.
The kit can quantify total glutathione
from 1-100 ng/well. For detecting lower
glutathione concentrations, such as in

DTNB

blood samples, increasing the reaction


time significantly improves the signal.
The kit can also specifically detect the reduced form of glutathione (GSH) when
glutathione reductase is omitted from
the reaction mixture.

Colorimetric Glutathione
Reductase Activity Kit
PromoKines Colorimetric Glutathione
Reductase Activity Kit was designed
for measuring glutathione reductase
(GR) activity in biological samples. It
is highly sensitive and well suited for
high-throughput. The assay is based on
the GR-catalysed reduction of GSSG to
GSH, which reacts with 5,5-Dithiobis
(2-nitrobenzoic acid) (DTNB) to generate the yellow TNB2-. The kit can detect
0.1 40 mU/ml GR in various samples.

2GSH

Glutathione
+ NADPH
reductase
2-Nitro-5-thiobenzoic acid

GSSG

Fig. 1: Principle of the Colorimetric Glutathione Detection Kit.

https://www.promokine.info/shop
Product

Size

Catalog Number

Fluorometric Glutathione Detection Kit

100 assays

PK-CA577-K251

Colorimetric Glutathione Detection Kit

100 assays

PK-CA577-K261

Colorimetric Glutathione Reductase


Activity Kit

200 assays

PK-CA577-K761

Note: Please check our website regularly for new cell-based apoptosis assays.

Apoptosis

Applications

132

7 Apoptosis

7.5 Annexin V Detection Kits


Applications





Screening for apoptotic cells


Differentiating apoptotic and
necrotic cells
Annexin detection
Flow cytometry
Fluorescence microscopy

Apoptosis

Features
High fluorescence intensity and
sensitivity
Many different labels and tags
available
Fast, convenient protocol suited for
automation and high-troughput
Excitation and emission spectra are
compatible with common excitation
sources and filter sets
In the early stages of apoptosis, changes
can be observed on the cell surface.
Cells lose their phospholipid membrane
asymmetry and expose phosphatidylserine (PS) on the exterior plasma membrane. Annexin V is a Ca2+-dependent,
phospholipid-binding protein with high
affinity for PS and is very useful for
identifying apoptotic cells that expose
PS on their surfaces to the extracellular
environment. Annexin V may be conjugated to biotin or fluorophores such as
FITC and phycoerythrin (PE). It can also

be tagged with EGFP (Enhanced Green


Fluorescent Protein). Since Annexin V
conjugates and tagged annexins retain
their high affinity for PS, they are ideally suited as highly sensitive probes to
identify early apoptotic cells and to detect apoptosis much earlier than assays
based on DNA fragmentation or loss of
membrane integrity. The stained cells
can be evaluated by flow cytometry or
fluorescence microscopy using common
filter sets and flow cytometer channels.

Annexin V Detection Kits


The Annexin V Apoptosis Detection
Kits include an Annexin V conjugate
or tagged Annexin V as well as binding buffer and in some cases Propidium
Iodide (PI) for differentiating between
apoptotic and dead cells. The kits allow
a one-step staining procedure that takes
only 10 minutes. In addition, the assay
can be performed directly on living cells.
Promokine Annexin V Apoptosis Detection KitPlus includes an additional dye, SYTOX green, that is impermeant to living
and apoptotic cells, but stains necrotic
cells with intense green fluorescence
by binding to cellular nucleic acids. After staining a cell population with e.g.,
fluorescent Annexin V-Cy3 or Annexin
V-PE conjugates, and SYTOX Green dye

in the provided binding buffer, apoptotic


cells show red fluorescence, necrotic cells
show green fluorescence, and live cells
show little or no fluorescence. These
populations can easily be distinguished
by fluorescence microscopy and flow cytometry using the appropriate filters and
channels.

Individual Annexin V Reagents


PromoKine also offers a wide range
of Annexin V reagents separately. For
easy staining of early apoptotic cells,
Annexin V conjugated to FITC, PE, Cy3,
Cy5, or biotin is available, as well as Annexin V tagged with EGFP. The stained
cells can be evaluated by flow cytometry
or fluorescence microscopy using common filter sets and flow cytometer channels. Biotinylated Annexin V can be detected in conjunction with conventional
dye-staining using any streptavidin- or
avidin-dye reagents such as PromoFluorstreptavidin conjugates, fluorescein-, PE-,
or APC-streptavidin as well as streptavidin/avidin conjugated to peroxidase, alkaline phosphatase (AP), b-galactosidase,
etc.
Please visit www.promokine.info to find
our complete range of individual Annexin
V reagents as well as Annexin V antibodies and ELISAs.

https://www.promokine.info/shop
Product

Size

Catalog Number

Annexin V-FITC Detection Kit

25 assays
100 assays
400 assays

PK-CA577-K101-25
PK-CA577-K101-100
PK-CA577-K101-400

Annexin V-FITC Detection KitPlus

25 assays
100 assays
400 assays

PK-CA577-K201-25
PK-CA577-K201-100
PK-CA577-K201-400

Annexin V-Cy3 Detection Kit

25 assays
100 assays
400 assays

PK-CA577-K102-25
PK-CA577-K102-100
PK-CA577-K102-400

Annexin V-Cy3 Detection KitPlus

25 assays
100 assays
400 assays

PK-CA577-K202-25
PK-CA577-K202-100
PK-CA577-K202-400

Annexin V-Cy5 Detection Kit

25 assays
100 assays
400 assays

PK-CA577-K103-25
PK-CA577-K103-100
PK-CA577-K103-400

Annexin V-EGFP Detection Kit

25 assays
100 assays
400 assays

PK-CA577-K104-25
PK-CA577-K104-100
PK-CA577-K104-400

7 Apoptosis

133

Product

Size

Catalog Number

Annexin V-PE Detection Kit

25 assays
100 assays
400 assays

PK-CA577-K128-25
PK-CA577-K128-100
PK-CA577-K128-400

Annexin V-PE Detection KitPlus

25 assays
100 assays
400 assays

PK-CA577-K203-25
PK-CA577-K203-100
PK-CA577-K203-400

Annexin V-PE-Cy5 Detection Kit

25 assays
100 assays
400 assays

PK-CA577-K129-25
PK-CA577-K129-100
PK-CA577-K129-400

Annexin V-Biotin Detection Kit

25 assays
100 assays
400 assays

PK-CA577-K109-25
PK-CA577-K109-100
PK-CA577-K109-400

Annexin V-FITC Reagent

200 assays
1000 assays

PK-CA577-1001-200
PK-CA577-1001-1000

Annexin V-Cy3 Reagent

200 assays
1,000 assays

PK-CA577-1002-200
PK-CA577-1002-1000

Annexin V-Cy5 Reagent

200 assays
1,000 assays

PK-CA577-1013-200
PK-CA577-1013-1000

Annexin V-EGFP Reagent

200 assays
1,000 assays

PK-CA577-1004-200
PK-CA577-1004-1000

Annexin V-PE Reagent

200 assays
1,000 assays

PK-CA577-1014-200
PK-CA577-1014-1000

Annexin V-PE-Cy5 Reagent

200 assays
1,000 assays

PK-CA577-1015-200
PK-CA577-1015-1000

Annexin V-Biotin Reagent

100 g
1 mg

PK-CA577-1003-100
PK-CA577-1003-1000

Annexin V Unlabeled Reagent

100 g

PK-CA577-1005-100

1X Annexin V Binding Buffer

100 ml

PK-CA577-1035

10X Annexin V Binding Buffer

100 ml

PK-CA577-1006

Note: Please check our website regularly for new cell-based apoptosis assays.

Apoptosis

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134

7 Apoptosis

Apoptosis

7.6 Caspase Kits


Caspases, a family of cysteine aspartic
acid proteases, are important factors
in the progression of apoptosis and
can be activated by various harmful
physiological stimuli such as cytochrome c
release due to mitochondrial membrane
disruption, DNA damage, ER stress,
or inducers such as TNF-alpha and
FasL. Caspases are normally present in
the cell as inactive pro-enzymes. During apoptosis, they are activated by
proteolysis forming a proteolytic cascade that results in the cleavage of distinct proteins that are essential for cell
viability.

7.6.1 Caspase Detection Kits

Caspase Staining Kits

Applications

The fluorometric Caspase Staining Kits


are the ideal tools for detecting activated
caspases in situ in living cells. The kits
utilize potent caspase inhibitors conjugated to FITC or rhodamine as fluorescent markers. The caspase inhibitors
are cell-permeable, nontoxic, and bind
specifically and irreversibly to the activated caspases in apoptotic cells. With
the FITC or rhodamine labels, activated
caspases in apoptotic cells can be directly
visualized by fluorescence microscopy or
analyzed by using flow cytometry, or a
fluorescence plate reader. Caspase inhibitors without FITC or rhodamine labels
are also included in the kits as negative
controls.
The components of the kits (e.g. the
Wash Buffer) are also available separately (please see www.promokine.info).

Screening for apoptotic cells


Flow cytometry and fluorescence
microscopy
Caspase detection

Features
High fluorescent intensity and
sensitivity
Highly specific
Fast and simple
Appropriate for various multiwell
formats and automation
Excitation and emission spectra are
compatible with common excitation
sources and filter sets

https://www.promokine.info/shop
Product

Size

Catalog Number

Green Multi-Caspase Staining Kit

25 assays
100 assays

PK-CA577-K180-25
PK-CA577-K180-100

Green Caspase-2 Staining Kit

25 assays
100 assays

PK-CA577-K182-25
PK-CA577-K182-100

Green Caspase-3 Staining Kit

25 assays
100 assays

PK-CA577-K183-25
PK-CA577-K183-100

Green Caspase-8 Staining Kit

25 assays
100 assays

PK-CA577-K188-25
PK-CA577-K188-100

Green Caspase-9 Staining Kit

25 assays
100 assays

PK-CA577-K189-25
PK-CA577-K189-100

Green Caspase-12 Staining Kit

25 assays
100 assays

PK-CA577-K172-25
PK-CA577-K172-100

Red Multi-Caspase Staining Kit

25 assays
100 assays

PK-CA577-K190-25
PK-CA577-K190-100

Red Caspase-3 Staining Kit

25 assays
100 assays

PK-CA577-K193-25
PK-CA577-K193-100

Red Caspase-8 Staining Kit

25 assays
100 assays

PK-CA577-K198-25
PK-CA577-K198-100

Red Caspase-9 Staining Kit

25 assays
100 assays

PK-CA577-K199-25
PK-CA577-K199-100

Note: Please check our website regularly for new cell-based apoptosis assays. For a complete list of active recombinant caspases,
caspase substrates, inhibitors, and antibodies please visit our website www.promokine.info, or see the PromoKine Price List.

7 Apoptosis

Caspase Drug Screening Kits

The PromoKine Caspase Screening Kit


(FC) provides a convenient means for
detecting the activation of caspases by
flow cytometry in living cells. The assay
is based on the cleavage of (aspartyl)2Rhodamine 110 (D2R), a reported substrate for members of caspase family
proteases. The caspase substrate D2R is
nonfluorescent. However, upon cleavage of the substrate by cellular activated
caspases, the released rhodamine 110
gives rise to fluorescence that can be
measured by flow cytometry (excitation
maximum at 488 nm, emission maximum at 530 nm).

Inhibition of caspases has been shown


to delay apoptosis, implicating a potential role in drug screening efforts.
PromoKine provides a full line of Caspase Drug Screening Kits for effective
screening of caspase inhibitors using
fluorometric methods. The assays utilize
peptide substrates containing consensus
cleavage sequences specific for each
caspase, labeled with AFC (7-amino4-trifluoromethyl coumarin). Active
caspases (provided in the kits) cleave
the synthetic substrates to release free

AFC which can then be quantified using


a fluorescence plate reader. Compounds
to be screened can be directly added to
the reaction, and the level of caspase
inhibition can then be determined. The
assays are simple, straightforward, and
can be performed directly in microtiter
plates. Assay conditions for each caspase
have been optimized to obtain the maximal activity.
Apoptosis

Caspase Screening Kit (FC)

135

https://www.promokine.info/shop
Product
Caspase Screening Kit (FC)

Size

Catalog Number

25 assays
100 assays

PK-CA577-K200-25
PK-CA577-K200-100

Caspase-1 Drug Screening Kit

100 assays

PK-CA577-K151-100

Caspase-2 Drug Screening Kit

100 assays

PK-CA577-K152-100

Caspase-3 Drug Screening Kit

100 assays

PK-CA577-K153-100

Caspase-4 Drug Screening Kit

100 assays

PK-CA577-K154-100

Caspase-5 Drug Screening Kit

100 assays

PK-CA577-K155-100

Caspase-6 Drug Screening Kit

100 assays

PK-CA577-K156-100

Caspase-7 Drug Screening Kit

100 assays

PK-CA577-K157-100

Caspase-8 Drug Screening Kit

100 assays

PK-CA577-K158-100

Caspase-9 Drug Screening Kit

100 assays

PK-CA577-K159-100

Caspase-10 Drug Screening Kit

100 assays

PK-CA577-K160-100

Note: Please check our website regularly for new cell-based apoptosis assays. For a complete list of active recombinant caspases,
caspase substrates, inhibitors, and antibodies please visit our website www.promokine.info, or see the PromoKine Price List.

136

7 Apoptosis

7.6.2 Caspase Quantification Kits


Applications
Apoptosis screening
Quantification of caspase activity

Apoptosis

Features
Highly sensitive and specific
Fast and convenient
Compatible with standard lab
equipment
Suited for automation

Caspase Fluorometric &


Colorimetric Assay Kits
With the Caspase Fluorometric & Colorimetric Assay Kits caspase activity in mammalian cells can be quantified by either
fluorescence or absorbance in a fast and
simple procedure. The kits utilize the caspase substrate (Ac-DEVD)2-R110 that is
fluorogenic as well as chromogenic. It
has an extinction coefficient that is 10
times higher than that of pNA - making
the assay extremely sensitive.

Caspase Fluorometric Assay Kits


The Caspase Fluorometric Assay Kits
are intended for convenient and sensitive quantification of caspase activity
in cell lysates. The kits utilize synthetic
peptides which mimic the optimal substrate for distinct caspases and are labeled with 7-amino-4-trifluoromethyl
coumarin (AFC). Activated caspases in
apoptotic cells specifically cleave their
synthetic substrates, resulting in the release of free AFC which is then quantified using a fluorometer. The kits are
fast, straight-forward, and adaptable to
96-well plates.

Caspase Colorimetric Assay Kits


The Caspase Colorimetric Assay Kits are
intended for convenient and sensitive
quantification of caspase activity in cell
lysates. The kits utilize synthetic peptides
which mimic the optimal substrate for
distinct caspases and are labeled with
p-nitroaniline (pNA). Activated caspases
in apoptotic cells specifically cleave their
synthetic substrates, resulting in the release of free pNA, which is then quantified using a spectrophotometer. The kits
are fast, straightforward, and adaptable
to 96-well plates.

Caspase Fluorometric Assay


Kits (HTS)
The Caspase Fluorometric Assay Kits
(HTS) were designed for economic highthroughput applications. They use the
fluorogenic and chromogenic substrate
(Ac-DEVD)2-R110 and include specific
caspase inhibitors as negative controls.

Caspase-3 and Caspase-7


Immunoassay Kits
Caspase-3 plays a key role in the
initiation of cellular events during the
apoptotic process. The PromoKine
Caspase-3 and Caspase-7 Immunoassay
Kits allow quantification of the specific
activity of caspase-3 and -7. Since caspase-3 and -7 share the same target
substrate sequence (DEVD), it is difficult
to differentiate the cleavage activity contributed by these two caspases in vitro.
Consequently, the assays utilize caspase-3 or -7 specific antibodies to capture the activated caspase-3 or -7 in cell
lysate. The specific activity of caspase-3
or -7 can then be analyzed using the
common caspase-3/7 substrate DEVDAFC. The assay system ensures specific
detection of caspase-3 or caspase-7 ac-

tivity in apoptotic samples. Other caspases and non-specific proteases are not
detected.

Individual Caspase Reagents


In addition to the complete caspase
staining and activity kits, PromoKine also
provides a variety of high quality, purified active recombinant caspases and
pro-caspases from human, mouse and
rat, as well as caspase substrates and
inhibitors. Optimized assay buffers and
other supplementary reagents for setting
up custom assays are also available separately. These reagents allow researchers
to perform numerous caspase assays
economically. Sets of ready-to-use caspase enzymes, caspase substrates, and
caspase inhibitors provide more convenience in setting up individual assays to
evaluate multiple caspases in the same
experiment.
PromoKines active caspases are very
useful for studying enzyme regulation
and kinetics, screening caspase inhibitors, determining target substrates, and
as positive controls in caspase activity
assays and Western blot analyses.
The caspase substrates come in readyto-use form for both fluorometric and
colorimetric caspase assays.
Please visit www.promokine.info or
see the PromoKine Price List to find the
complete range of PromoKine individual
caspase reagents.
A range of caspase antibodies and
ELISAs can also be found on our website.

7 Apoptosis

137

Product

Size

Catalog Number

Caspase-1 Fluorometric Assay Kit

25, 100, 200, 400 assays

PK-CA577-K110-XXX*

Caspase-2 Fluorometric Assay Kit

25, 100, 200, 400 assays

PK-CA577-K116-XXX*

Caspase-3 Fluorometric Assay Kit

25, 100, 200, 400 assays

PK-CA577-K105-XXX*

Caspase-4 Fluorometric Assay Kit

25, 100, 200 assays

PK-CA577-K126-XXX*

Caspase-5 Fluorometric Assay Kit

25, 100, 200, 400 assays

PK-CA577-K122-XXX*

Caspase-6 Fluorometric Assay Kit

25, 100, 200, 400 assays

PK-CA577-K114-XXX*

Caspase-8 Fluorometric Assay Kit

25, 100, 200, 400 assays

PK-CA577-K112-XXX*

Caspase-9 Fluorometric Assay Kit

25, 100, 200, 400 assays

PK-CA577-K118-XXX*

25, 100 assays

PK-CA577-K124-XXX*

Caspase-10 Fluorometric Assay Kit


Caspase-12 Fluorometric Assay Kit

25, 100 assays

PK-CA577-K139-XXX*

Caspase-1 Colorimetric Assay Kit

25, 100, 200, 400 assays

PK-CA577-K111-XXX*

Caspase-2 Colorimetric Assay Kit

25, 100, 200, 400 assays

PK-CA577-K117-XXX*

Caspase-3 Colorimetric Assay Kit

25, 100, 200, 400 assays

PK-CA577-K106-XXX*

Caspase-4 Colorimetric Assay Kit

25, 100, 200 assays

PK-CA577-K127-XXX*

Caspase-5 Colorimetric Assay Kit

25, 100, 200, 400 assays

PK-CA577-K123-XXX*

Caspase-6 Colorimetric Assay Kit

25, 100, 200, 400 assays

PK-CA577-K115-XXX*

Caspase-8 Colorimetric Assay Kit

25, 100, 200, 400 assays

PK-CA577-K113-XXX*

Caspase-9 Colorimetric Assay Kit

25, 100, 200, 400 assays

PK-CA577-K119-XXX*

25, 100 assays

PK-CA577-K125-XXX*

25 assays
100 assays

PK-CA707-30011-1
PK-CA707-30011-2

1 ml
10 ml
100 ml

PK-CA707-30012-1
PK-CA707-30012-2
PK-CA707-30012-3

Caspase-3 Immunoassay Kit

100 assays

PK-CA577-K163

Caspase-7 Immunoassay Kit

100 assays

PK-CA577-K167

Caspase-10 Colorimetric Assay Kit


Caspase-8 Fluorometric & Colorimetric Assay Kit
Caspase-8 Fluorometric Assay Kit (HTS)

Note: Please check our website regularly for new cell-based apoptosis assays. For a complete list of active recombinant caspases,
caspase substrates, inhibitors, and antibodies please visit our website www.promokine.info or see the PromoKine Price List.

* XXX stands for the respective package size: 25, 100, 200 or 400 tests.

Apoptosis

https://www.promokine.info/shop

138

7 Apoptosis

7.7 Cathepsin/Calpain Detection Kits


Applications
Screening for apoptotic cells

Apoptosis

Features





Non-radioactive method
Easy and fast procedure
Appropriate for various multiwell
formats and automation
Compatible with standard lab equipment
and various multi-well formats

Apoptosis can be mediated by mechanisms other than the well-studied caspase-mediated cleavage cascade. There
is growing knowledge that alternative
proteolytic enzymes such as the lysosomal cathepsin proteases may initiate
or propagate proapoptotic signals, but
it is currently unclear how cathepsin
mediates apoptosis.

Cathepsin Activity Assay and


Cathepsin Inhibitor Screening Kits
The Cathepsin Activity Assay Kits are
based on fluorometric or colorimetric
(Cathepsin G assay) detection and utilize the preferred substrate sequence
for each cathepsin, labeled with AFC

(amino-4-trifluoromethylcoumarin), MCA
(7-amino-4-methyl coumarin) or pNA
(p-nitroaniline). Cell lysates and other
samples that contain cathepsins cleave
the synthetic substrate to release free
AFC, MCA or pNA that can be easily
quantified using a fluorometer/photometer or a fluorescence plate reader/
ELISA reader. The cathepsin assays are
easy to perform and can be adapted to
96-well plate assays. Assay conditons
have been optimized to obtain the maximal activity.
The fluorometric Cathepsin D Inhibitor
Screening Kit includes active Cathepsin
D enzyme and a synthetic Cathepsin
D substrate coupled to MCA. It is a
simple, straightforward assay that can
be adapted to 96-well plate assays and
high-throughput screening. The relative
efficacy of test inhibitors are compared
to the positive control inhibitor, Pepstatin A.
A range of Cathepsin antibodies and
ELISAs can also be found on our website.

processes (e.g., apoptosis). The Calpain


Activity Assay Kit provides a simple and
convenient means for analysing calpain
activity in apoptotic cells and other
samples. The assay is based on fluorometric detection and quantitation of the
cleavage of a calpain substrate at 505
nm using a fluorometer or a fluorescence
plate reader. The kit contains positive
and negative controls. Comparison of
the fluorescence intensity from a treated
sample with a normal control allows
determination of the changes in calpain
activity.
The fluorometric Calpain-1 Inhibitor
Screening Kit includes active Calpain-1
enzyme and a synthetic Calpain-1
substrate. It provides a rapid, simple,
sensitive, and reliable test suitable for
high-throughput screening of Calpain-1
inhibitors. In the presence of potent Calpain-1 inhibitors, the hydrolyzation of
the calpain substrate will be impeded.
An inhibitor control is included to compare the efficacy of test inhibitors.

Calpain Activity Assay and Calpain


Inhibitor Screening Kits

A range of different Calpain antibodies


can also be found on our website.

The activation of calpain is involved in


many physiological and pathological
https://www.promokine.info/shop

Product
Cathepsin B Activity Assay Kit

Size

Catalog Number

100 assays

PK-CA577-K140

Cathepsin D Activity Assay Kit

100 assays

PK-CA577-K143

Cathepsin D Inhibitor Screening Kit

100 assays

PK-CA577-K148

Cathepsin G Activity Assay Kit

100 assays

PK-CA577-K146

Cathepsin H Activity Assay Kit

100 assays

PK-CA577-K145

Cathepsin K Activity Assay Kit

100 assays

PK-CA577-K141

Cathepsin L Activity Assay Kit

100 assays

PK-CA577-K142

Cathepsin S Activity Assay Kit

100 assays

PK-CA577-K144

Calpain Activity Assay Kit

100 assays

PK-CA577-K240

Calpain-1 Inhibitor Screening Kit

100 assays

PK-CA577-K244

Cathepsin B, human (native)

5 g

PK-RP577-1021

Cathepsin D, human (native)

5 g

PK-RP577-1022

Cathepsin H, human (native)

5 g

PK-RP577-1023

5 g
100 g

PK-RP577-1135-5
PK-RP577-1135-100

5 g

PK-RP577-1025

Cathepsin L, human, recombinant


Cathepsin S, human (native)
Calpain I, human (native)
Cathepsin B & L Substrate Z-FR-R110
Cathepsin Substrate Z-FR-AMC

100 g

PK-RP577-1134

5 mg

PK-CA707-10209

25 mg

PK-CA707-10201

Calpain Inhibitor Z-LLY-FMK

20 l (10 mM)

PK-CA577-1125-20C

Cathepsin B&L Inhibitor Z-Phe-Phe-FMK

20 l (10 mM)

PK-CA577-1126-20C

7 Apoptosis

139

7.8 Kinase Detection Kits

Screening for apoptotic cells


Kinase detection and activity
screening

Features
Non-radioactive method
Easy, accurate, and fast procedure
Compatible with standard lab
equipment

large numbers of samples. The assay


utilizes an N-terminal c-Jun fusion protein bound to Glutathione-Sepharose
beads to selectively pull down JNK
from cell lysate. After a washing step to
remove non-specifically bound proteins,
the kinase reaction is carried out in the
presence of cold ATP. c-Jun phosphorylation is then determined by Western blot
analysis using a phospho-c-Jun specific
antibody.

JNK Activity Detection Kit


The activation of protein kinases, such
as JNK and Akt, plays an important role
in apoptosis and other physiological and
pathological processes. PromoKine provides a novel non-radioactive system for
convenient measurement of JNK and
Akt activities.

JNK Activity Screening Kit


The JNK Activity Screening Kit can
screen JNK activity quickly even with

The JNK Activity Detection Kit utilizes


a JNK-specific antibody to immunoprecipitate JNK from cell lysates. The
activity of the JNK is then detected in a
kinase reaction using recombinant c-Jun
as substrate. c-Jun phosphorylation is
determined by Western blotting using
a phospho-c-Jun specific antibody. The
kit specifically detects JNK activity; other
kinase activities are not detected.

Akt Activity Detection Kit


The Akt Activity Detection Kit utilizes
an Akt-specific antibody to immunoprecipitate Akt from cell lysates. Akt-specific
activity is then detected in a kinase reaction using recombinant GSK-3a as
a substrate. Phosphorylation levels of
GSK-3a are determined by Western blot
analysis using a phospho-GSK-3a specific antibody included in the kit. The kit
specifically detects Akt1, Akt2, and Akt3
activities. Other kinase activities are not
detected.
Active JNK and Akt kinases as well
as JNK/Akt substrates, activators, inhibitors, and control cell lysates are also
available separately.
Please visit www.promokine.info or see
the PromoKine Price List to find more
active kinases that can be used for
cell analysis and apoptosis research. A
range of kinase antibodies can also be
found on our website.

https://www.promokine.info/shop
Product

Size

Catalog Number

JNK Activity Screening Kit

40 assays

PK-CA577-K430

JNK Activity Detection Kit

40 assays

PK-CA577-K431

Akt Activity Detection Kit

40 assays

PK-CA577-K435

1 mg

PK-CA577-1701

Akt Inhibitor
Chk2 Inhibitor

1 mg

PK-CA577-1702

c-Jun (1-79)-GST Fusion Protein

100 ug

PK-CA577-7001

GSK-3a-GST Fusion Protein

100 ug

PK-CA577-7003

GSK-3, human, recombinant

100 ug

PK-CA577-7004

L-JNK Peptide Inhibitor I

50 l (1 mM)

PK-CA577-7011

L-JNKI1 Negative Control Peptide

50 l (1 mM)

PK-CA577-7021

JNK Negative Control Cell Lysate

1 mg

PK-CA577-7031

JNK Activated Cell Lysate

1 mg

PK-CA577-7032

Akt Negative Control Cell Lysate

1 mg

PK-CA577-7035

Akt Activated Cell Lysate

1 mg

PK-CA577-7036

Note: Please check our website regularly for new cell-based apoptosis assays. For a complete list of active recombinant kinases,
kinase substrates, inhibitors, and antibodies please visit our website www.promokine.info or see the PromoKine Price List.

Apoptosis

Applications

140

7 Apoptosis

7.9 Apoptotic and Necrotic Cell Detection


Applications



Simultaneous detection of apoptotic,


necrotic, and healthy cells
Fluorescence microscopy
Flow cytometry

Apoptosis

Features
Convenient ready-to-use kit
Fast procedure
Highly sensitive

Apoptosis and necrosis, the two major


processes leading to cell death, show
several distinct differences. One characteristic of apoptotic cells is the translocation of phosphatidylserine from the
inner to the outer side of the plasma
membrane. This allows phagocytic cells
to recognize and destroy apoptotic
cells but can also be used for identifying apoptotic cells. Necrotic cells, on
the other hand, are characterized by a
loss of membrane integrity. This allows
membrane-impermeable probes to enter
and stain them.

Apoptotic/Necrotic Cells
Detection Kit
The Apoptotic/Necrotic Cells Detection
Kit provides convenient quantification of
apoptotic (green) and necrotic (red) cells

within the same cell population by flow


cytometry or fluorescence microscopy.
For tagging apoptotic cells, fluorescein
labeled Annexin V is used. Annexin V
specifically binds to the phosphatidylserine exposed on the outer membrane of
apoptotic cells. In order to mark necrotic
cells, Ethidium homodimer III (EthD-III)
is utilized. This fluorescent DNA binding substance can enter necrotic cells
because of the damaged plasma membrane, but not living or apoptotic cells.
Once in the necrotic cell, it binds to cellular DNA and fluoresces red. Due to its
significantly higher fluorescence quantum yield, Ethidium homodimer III is
superior to propidium iodide or Ethidium
homodimer I.

Fig. 2: Apoptotic U937 cells stained


with Annexin V-FITC using PromoKines
Apoptotic/Necrotic/Healthy Cells Detection Kit.

Apoptotic/Necrotic/Healthy Cells
Detection Kit
The Apoptotic/Necrotic/Healthy Cells
Detection Kit provides convenient quantification of apoptotic (green), necrotic
(red), and healthy cells (blue) within the
same cell population by flow cytometry
or fluorescence microscopy. In addition
to the fluorescent dyes utilized in the
Apoptotic/Necrotic Cells Detection Kit,
it also contains a membrane-permeable
blue fluorescent DNA stain (Hoechst
33342) for quantifying healthy cells.

Fig. 3: Apoptotic/Necrotic U937 cell


stained with Ethidium homodimer III
(EthD-III) using PromoKines Apoptotic/
Necrotic/Healthy Cells Detection Kit.

https://www.promokine.info/shop
Product

Size

Catalog Number

Apoptotic/Necrotic Cells Detection Kit

50 assays

PK-CA707-30017

Apoptotic/Necrotic/Healthy Cells
Detection Kit

50 assays

PK-CA707-30018

7 Apoptosis

141

Applications
Isolation of apoptotic cells
Removal of dead cells from culture

Features
Fast, simple, and efficient
Complete ready-to-use kit
The PromoKine Apoptotic Cell Isolation
Kit provides a simple and efficient means
for isolating apoptotic cells or removing

MagBeads adhere to the magnet while normal cells stay in suspension.


The bound apoptotic cells can then be
released from the MagBeads using the
Elution Buffer provided in the kit. The
separated apoptotic cells and normal
cells can be used in a variety of assays to
study apoptotic mechanisms and pathways. The kit has also been successfully
used to remove dead cells from normal
cultures.
A re-usable magnetic separator optimized for the kit is also available.

dead cells from cell culture or tissue


preparations using Annexin V magnetic
beads (MagBeads). Since Annexin V is a
phospholipid-binding protein with high
affinity for phosphatidylserine (PS), it
binds strongly to the PS that is redistributed from the inner to the outer surface
of the plasma membrane during apoptosis. Annexin V-biotin first binds to apoptotic cells. Then, biotin binds to streptavidin-MagBeads, which enables the
separation of apoptotic cells from normal
cells. The apoptotic cells bound to the

https://www.promokine.info/shop
Product
Apoptotic Cell Isolation Kit

Size

Catalog Number

30 isolations

PK-CA577-K258

PK-CA577-K1999-1

Magnetic Separator (re-usable)

7.11 Apoptosis Inducers


Applications
Induction of apoptotic processes
Ideally suited for use with diverse
apoptosis detection systems

Features
High quality
Ready-to-use

PromoKine offers a select choice of high


quality apoptosis inducers in ready-touse form. Some of them are also available
in a convenient set. The apoptosis inducers are well-characterized and effective
in disrupting mitochondrial transmembrane potential and activating caspases
as well as inducing phosphatidyl-serine
(PS) exposure, DNA fragmentation, and
other apoptotic characteristics. All inducers are ideally suited for use with the

various PromoKine apoptosis detection


systems.
PromoKine provides a wide range of
high quality growth factors, cytokines,
ELISAs, and antibodies that are relevant
to apoptosis research (see our website).

https://www.promokine.info/shop
Product

Size

Catalog Number

50 l

PK-CA577-1036-50

Actinomycin D

5 mg
50 mg

PK-CA577-1036-5MG
PK-CA577-1036-50MG

Anisomycin

10 mg

PK-CA577-1549-10

1 kit

PK-CA577-K121-5

25 mg

PK-CA577-1552-25

5 mg

PK-CA577-1560-5

50 mg
500 mg

PK-CA577-1039-50MG
PK-CA577-1039-500MG

Actinomycin D (10 mM)

Apoptosis Inducer Set


Betulinic acid
Brefeldin A
Camptothecin

Apoptosis

7.10 Apoptotic Cell Isolation Kit

142

7 Apoptosis

https://www.promokine.info/shop
Product
Camptothecin (2 mM)
Carboplatin

Catalog Number

1 ml

PK-CA577-1039-1

100 mg

PK-CA577-1553-100

Colchicine

1g

PK-CA577-1519

Cycloheximide

1g

PK-CA577-1041-1G

Cycloheximide (100 mM)

1 ml

PK-CA577-1041-1

10 mg
50 mg

PK-CA577-1524-10
PK-CA577-1524-50

Dexamethasone

1g
10 g

PK-CA577-1042-1G
PK-CA577-1042-10G

Dexamethasone (10 mM)

1 ml

PK-CA577-1042-1

Daunorubicin.HCl
Apoptosis

Size

Doxorubicin.HCl
Etoposide
Etoposide (10 mM)
Genistein

5 mg

PK-CA577-1527-5

25 mg
250 mg

PK-CA577-1043-25MG
PK-CA577-1043-250MG

100 l

PK-CA577-1043-100

10 mg
100 mg

PK-CA577-1533-10
PK-CA577-1533-100

Ionomycin (calcium salt)

5 mg

PK-CA577-1566-5

Ionomycin (free acid)

5 mg

PK-CA577-1565-5

Mifepristone

50 mg

PK-CA577-1561-50

Mitomycin C

5 mg
10 mg
100 mg

PK-CA578-M1108-5
PK-CA578-M1108-10
PK-CA578-M1108-100

Okadaic acid

25 g

PK-CA577-1543-25

Phorbol-12-myristate 13-acetate (PMA)

5 mg

PK-CA577-1544-5

5 mg
50 mg

PK-CA577-1568-5
PK-CA577-1568-50

Rosiglitazone

5 mg
100 mg

PK-CA577-1559-5
PK-CA577-1559-100

Staurosporine

100 g
1 mg

PK-CA577-1048-010
PK-CA577-1048-1

1g

PK-CA577-1551

Rapamycin

Tamoxifen citrate
Thapsigargin

1 mg

PK-CA577-1558-1

Tyrphostin AG 1295

5 mg

PK-CA577-1571-5

Tyrphostin AG 490

5 mg

PK-CA577-1570-5

Note: Please check our website regularly for new apoptosis inducers.

Fluorescent Labeling
The availability of new fluorophores has dramatically changed
the possibilities for the sensitive detection of biomolecules and
the analysis of their interactions. Improved fluorescent dyes are
now enabling previously impossible studies of cellular structures and processes. PromoKine provides a wide range of highquality fluorophores for easy labeling of diverse biomolecules
including proteins, antibodies, and DNA, as well as complete
labeling kits and ready-to-use fluorescent conjugates.

144 Content Chapter 8

3
8

Fluorescent Labeling
8.1

PromoFluor Dyes . . . . . . . . . . . . . . . . . . . 145

8.2

PromoFluor Labeling Kits . . . . . . . . . . . . . 148

8.2.1

PromoFluor Biomolecules Labeling Kits . . . . 148

8.2.2

PromoFluor DNA Labeling Kits . . . . . . . . . . 149

8.3

PromoFluor Labeled Biomolecules . . . . . . 150

8.3.1

PromoFluor Labeled Antibodies . . . . . . . . . 150

8.3.2

PromoFluor Labeled Phalloidin . . . . . . . . . . 150

8.3.3

PromoFluor Labeled Nucleotides . . . . . . . . . 151

8.3.4

PromoFluor Labeled Biotin and Streptavidin 152

8.4

Phycobiliprotein Dyes . . . . . . . . . . . . . . . . 152

8.5

PromoFluor Antifade Reagent . . . . . . . . . 153

8 Fluorescent Labeling

145

8.1 PromoFluor Dyes

Labeling biomolecules such as


proteins, antibodies, and nucleic acids
Fluorescence microscopy
Flow cytometry
Fluorescent in situ hybridization
(FISH)
Fluorescence correlation spectroscopy
(FCS)
DNA and protein microarrays

Features









Strong light absorption and high


fluorescence intensity
Very photostable
Highly fluorescent over a broad pH
range (pH 4 - 9)
Water-soluble without the need to
use organic solvents
Excitation and emission spectra are
compatible with common excitation
sources and filter sets

PromoFluor dyes are powerful fluorophores spanning almost the whole visible
and infrared light spectrum (see Table 1).
They are superior to many other commonly used fluorescent dyes and well

qualified for a wide range of cell and


molecular biology applications. In addition to the standard dyes, specifically
designed PromoFluor-LSS dyes offer an
extra large Stokes shift, which makes
them ideally suited for multicolor labeling and flow cytometry applications.
All PromoFluor dyes are provided
either as free carboxylic acids or in
three reactive variants ready for covalent coupling to biomolecules:
amine-reactive NHS-Esters, AminoModified Labels, and thiol-reactive
Maleimides.

PromoFluor NHS-Esters
PromoFluor NHS-Esters (N-hydroxysuccinimidyl-esters) are intended for covalently labeling biomolecules containing a
primary amino group (all peptides/proteins contain at least one amino group
at the N-terminus). Moreover, amino
groups can also be incorporated in other
biomolecules without changing their
activity or functionality. The most common examples are 5-amino-modified
DNA oligomers and cDNA containing
aminoallyl-dU.

PromoFluor Amino-Modified Labels


PromoFluor Amino-Modified Labels are
intended for the covalent labeling of biomolecules containing a carboxy group
e.g., glycoproteins. The amino group
of PromoFluor Amino-Modified Labels
can be cross-coupled to carboxy groups
through a mild in situ activation.

PromoFluor Maleimides
PromoFluor Maleimides are intended for
covalently labeling biomolecules containing a thiol group (e.g. proteins). A
neutral thiol-reactive label has little influence on the isoelectric point of a protein.
In effect, PromoFluor Maleimides have
an advantage over amino group labels
because they do not interfere with techniques like differential 2D gel electrophoresis. Since most proteins contain
only a few thiol functional groups, thiolreactive dyes can be used to selectively
modify a protein or peptide containing
a thiol group at a specific site. Moreover,
these dyes are well suited for labeling
oligonucleotides. Maleimides couple to
thiol groups under very mild conditions
at a pH around 7.

Fig. 1: Left: Cell structures of HeLa cells visualized using primary anti-beta-tubulin antibodies and secondary PromoFluor 488-labeled antibodies (green) as well as primary anti-centromere antibodies and secondary PromoFluor 547-labeled antibodies (red).
Middle: Rat cytokeratin (green) detected with primary rabbit anti-cytokeratin antibodies and secondary anti-rabbit IgGs labeled
with PromoFluor 488. Actin (red) visualized using mouse anti-actin antibodies and secondary goat anti-mouse IgGs labeled with
PromoFluor 520LSS. Right: Solid-state fluorescence of PromoFluor 633.

Fluorescent
Labeling

Applications

146

8 Fluorescent Labeling

Fluorescent
Labeling

Name

Absorption
Maximum
[nm]

Emission
Maximum
[nm]

Description

Laser Excitation Source*

PromoFluor 350

354

431

Alternative to Alexa Fluor 350,


AMCA, AMCA-X

PromoFluor 405

400

420

Alternative to Alexa Fluor 405, Pacific Blue,


Cascade Blue

Blue diode laser

PromoFluor 415

418

467

Alternative to Pacific Blue, Spectrum Aqua,


and Diethylaminocoumarin

Blue diode laser

PromoFluor 488LSS

485

560

Dye with extra large Stokes shift.


Ideally suited for multicolor labeling
and flow cytometry applications

Argon 488 laser

PromoFluor 488

493

521

Alternative to Fluorescein (FITC), Alexa


Fluor 488 and CyTM2

Argon 488 laser

PromoFluor 488
Premium

490

516

Very photostable alternative to Fluorescein


(FITC), Alexa Fluor 488, CyTM2

Argon 488 laser

PromoFluor 500LSS

500

630

Dye with extra large Stokes shift.


Ideally suited for multicolor labeling
and flow cytometry applications

Argon 488 laser

PromoFluor 505

505

530

Alternative to Rhodamine GreenTM

Argon 488 laser

PromoFluor 510LSS

509

590

Dye with extra large Stokes shift.


Ideally suited for multicolor labeling
and flow cytometry applications

Argon 514 laser

PromoFluor 514LSS

515

650

Dye with extra large Stokes shift.


Ideally suited for multicolor labeling
and flow cytometry applications

Argon 514 laser

PromoFluor 520LSS

520

664

Dye with extra large Stokes shift.


Ideally suited for multicolor labeling
and flow cytometry applications

Argon 514 laser

PromoFluor 532

539

561

Alternative to Alexa Fluor 532,


Tetramethylrhodamine (TMR and TRITC)

Nd:YAG laser

PromoFluor 546

551

572

Alternative to Alexa Fluor 546,


Tetramethylrhodamine (TMR and TRITC)

Helium-Neon 543 laser

PromoFluor 555

557

574

Alternative to Alexa Fluor 555, CyTM3,


and Tetramethylrhodamine (TMR)

Helium-Neon 543 laser

PromoFluor 590

581

598

Alternative to Texas Red

Helium-Neon 594 laser

PromoFluor 610

610

630

Alternative to Texas Red

Helium-Neon 594 laser

PromoFluor 633

637

657

Alternative to Alexa Fluor 633

Helium-Neon 633 laser


and 635 diode laser

PromoFluor 647

653

672

Alternative to Alexa Fluor 647,


CyTM5, and Allophycocyanin

Krypton-Argon 647
laser

PromoFluor 670

673

694

Alternative to Alexa Fluor 680 and CyTM5.5

Helium-Neon laser

Argon laser or
UV-light

PromoFluor 680

690

709

Alternative to Alexa Fluor 680, Cy 5.5

Helium-Neon laser

PromoFluor 700

704

723

Alternative to Alexa Fluor 700

Far-red diode lasers

PromoFluor 750

748

772

Alternative to Alexa Fluor 750, CyTM7

Far-red diode lasers

PromoFluor 770

769

796

Alternative to Alexa Fluor 750 and CyTM7

Xenon-arc lamp or
dye-pumped laser

PromoFluor 780

783

800

Near infrared dye allowing for deep tissue


penetration and low background

Xenon-arc lamp or
dye-pumped laser

PromoFluor 840

844

Near infrared dye allowing for deep tissue


penetration and low background

Xenon-arc lamp or
dye-pumped laser

TM

Table 1: List of currently available PromoFluor dyes (for new PromoFluor dyes that may be added in the future please visit our website).
*PromoFluor dyes are compatible with common excitation sources including diode lasers, LEDs, tungsten lamps, xenon arc lamps, and custom made systems.

8 Fluorescent Labeling

147

Excitation / Emission [%]


100
80
60

Fig. 2: Excitation (left) and


emission (right) spectra of
PromoFluor 488LSS.

Fluorescent
Labeling

40
20

300

350

400

450

500

550

600

650

700

Wavelength [nm]

PromoFluor ChipPacks
PromoFluor ChipPacks are pre-packed
PromoFluor dyes intended to label

cDNA for microarray assays. The packs


contain 20 g aliquots of PromoFluor
555 and PromoFluor 647 NHS-Ester.
These aliquots are sufficient for labeling

the amount of cDNA synthesized in a


standard reverse transcription assay with
enzymatic incorporation of aminoallyldUTP.

https://www.promokine.info/shop
Product

Size

Catalog Number*

PromoFluor Carboxylic Acid

1 mg
5 mg

PK-PFXXX-0-01
PK-PFXXX-0-05

PromoFluor NHS-Ester

1 mg
5 mg

PK-PFXXX-1-01
PK-PFXXX-1-05

PromoFluor Amino-Modified Label

1 mg
5 mg

PK-PFXXX-2-01
PK-PFXXX-2-05

0.5 mg
1 mg
5 mg

PF-PFXXX-3-005
PK-PFXXX-3-01
PK-PFXXX-3-05

20 labeling reactions
50 labeling reactions
100 labeling reactions

PK-PF-CP-S
PK-PF-CP-M
PK-PF-CP-L

PromoFluor Maleimide

PromoFluor ChipPack

Coupling protocols for proteins are available on our website: www.promokine.info


Note: Different standard sizes are available for PF-488 and PF-505 carboxylic acids and NHS esters.

* XXX- stands fo the respective PromoFluor dye (e.g. PF-350, PF-405, PF-415 etc., see Table 1). Please see our website for an up-to-date list of available
PromoFluor Dyes.

148

8 Fluorescent Labeling

8.2 PromoFluor Labeling Kits

Fluorescent
Labeling

8.2.1 PromoFluor Biomolecules Labeling Kits


Applications

Features

Labeling biomolecules such as


proteins, antibodies, and nucleic acids
Fluorescence microscopy
Flow cytometry
Fluorescent in situ hybridization
(FISH)
Fluorescence correlation spectroscopy
(FCS)

Convenient ready-to-use kits


Contain superior PromoFluor dyes
(see page 146)
Highly efficient labeling
Very bright and stable fluorescent
signals

PromoFluor Biomolecules Labeling Kits


were developed for the convenient and
highly efficient labeling of target proteins or antibodies with PromoKines
superior PromoFluor dyes. They contain
the respective PromoFluor NHS-Ester
as well as all the other components required for labeling biomolecules.
PromoFluor Biomolecules Labeling Kits
work with all molecules with a primary
amino group such as peptides, proteins,
5-aminomodified DNA oligomers, and
cDNA containing aminoallyl-dU.

Fig. 3: Cell structures labeled with


antibodies bound to green-fluorescing
PromoFluor 505 (left) and red-fluorescing PromoFluor 647 (red) dyes.
Illustrations kindly provided by Covalys
Biosciences AG, Switzerland.

https://www.promokine.info/shop
Product
PromoFluor-XXX-Labeling-Kit

Size

Catalog Number*

5 reactions
10 reactions

PK-PF-LK-XXX-05
PK-PF-LK-XXX-10

*XXX- stands fo the respective PromoFluor dye (PF-405, PF-415, PF-488P, PF-488LSS, PF-514LSS, PF-546, PF-555, PF-633, PF-647, PF-670, PF-750,
PF-770; please see our website for an up-to-date list of available PromoFluor Labeling Kits).

8 Fluorescent Labeling

149

Applications



DNA labeling by PCR


DNA labeling by nick translation
Labeling of probes for FISH, micro-
arrays, Southern hybridizations, etc.

Features
Convenient and fast DNA labeling
Optimal incorporation of labeled
dUTPs
Highly fluorescent and stable fluoro-
phores

PromoKines PCR Labeling Kits and Nick
Translation Labeling Kits were designed
for convenient direct DNA labeling by
PCR or nick translation using Taq polymerase or DNA PolymeraseI/DNaseI.
The fluorescently labeled dUTP analogs provided with the kits have been
optimized for enzymatic incorporation
into DNA by using a proprietary linker
technology. The high incorporation rates

of the dye-dUTP analogs combined with


the outstanding stability and quantum
yield of the fluorophores make the kits
the ideal choice for all typical DNA labeling applications such as FISH, single
molecule detection, microarray gene
expression profiling, and other nucleic
acid hybridization assays. PromoKines
PCR Labeling Kits contain all reagents
required for highly efficient, rapid, and
easy-to-perform DNA labeling (excluding primers and template DNA). The
Nick Translation Labeling Kits contain all
reagents required for efficient, rapid and
easy-to-perform DNA labeling by nick
translation (excluding template DNA).
The labeled dUTPs are also available
separately (see page 151).
In addition, we also provide a kit for nonfluorescent labeling of DNA with biotin.
The biotinylated DNA can then interact
with streptavidin or strepatavidin conjugates (see page 152) for detection or
purification applications.

Fluorescent
Labeling

8.2.2 PromoFluor DNA Labeling Kits

Fig 4: PromoKines DNA Labeling Kits


have been used to generate fluorescently labeled DNA probes for staining
specific chromosome regions.

https://www.promokine.info/shop
Product

Product Description

Size

Catalog Number

PromoFluor-430 PCR Labeling Kit

Blue-green fluorescent DNA labeling


by PCR

10 reactions
50 reactions

PK-PF430-PCRLK-10
PK-PF430-PCRLK-50

PromoFluor-500 PCR Labeling Kit

Green-fluorescent DNA labeling


by PCR

10 reactions
50 reactions

PK-PF500-PCRLK-10
PK-PF500-PCRLK-50

PromoFluor-550 PCR Labeling Kit

Yellow-fluorescent DNA labeling


by PCR

10 reactions
50 reactions

PK-PF550-PCRLK-10
PK-PF550-PCRLK-50

PromoFluor-594 PCR Labeling Kit

Orange-fluorescent DNA labeling


by PCR

10 reactions
50 reactions

PK-PF594-PCRLK-10
PK-PF594-PCRLK-50

PromoFluor-640 PCR Labeling Kit

Red-fluorescent DNA labeling


by PCR

10 reactions
50 reactions

PK-PF640-PCRLK-10
PK-PF640-PCRLK-50

TexasRed PCR Labeling Kit

Orange-fluorescent DNA labeling


by PCR

10 reactions
50 reactions

PK-PFTXR-PCRLK-10
PK-PFTXR-PCRLK-50

Biotin PCR Labeling Kit

Kit for biotin-labeling of DNA by PCR

100 reactions
500 reactions

PK-CA-BIO-LK100
PK-CA-BIO-LK500

PromoFluor-430 Nick Translation


Labeling Kit

Blue-green fluorescent DNA labeling


by nick translation

10 reactions
50 reactions

PK-PF430-NTLK-10
PK-PF430-NTLK-50

PromoFluor-500 Nick Translation


Labeling Kit

Green-fluorescent DNA labeling


by nick translation

10 reactions
50 reactions

PK-PF500-NTLK-10
PK-PF500-NTLK-50

PromoFluor-550 Nick Translation


Labeling Kit

Yellow-fluorescent DNA labeling


by nick translation

10 reactions
50 reactions

PK-PF550-NTLK-10
PK-PF550-NTLK-50

PromoFluor-594 Nick Translation


Labeling Kit

Orange-fluorescent DNA labeling


by nick translation

10 reactions
50 reactions

PK-PF594-NTLK-10
PK-PF594-NTLK-50

PromoFluor-640 Nick Translation


Labeling Kit

Red-fluorescent DNA labeling


by nick translation

10 reactions
50 reactions

PK-PF640-NTLK-10
PK-PF640-NTLK-50

Note: Please see our website for an up-to-date list of available PromoFluor DNA Labeling Kits.

150

8 Fluorescent Labeling

8.3 PromoFluor Labeled Biomolecules


8.3.1 PromoFluor Labeled Antibodies
Applications

Fluorescent
Labeling

Fluorescence microscopy
Flow cytometry
Fluorescent in situ hybridization
(FISH)
Fluorescence correlation spectroscopy
(FCS)

Features



Ready-to-use antibody conjugates of


all PromoFluor dyes (see page 146) are
.
available as goat anti-human IgGs, goat
anti-rabbit IgGs, and goat anti-mouse
IgGs. The labeled secondary antibodies
(polyclonal) are affinity-purified and
dissolved in 100 mM PBS (pH 7.5) at a
concentration of 1 mg/ml. Each antibody
comes with a batch-specific absorption
spectrum.
Fig 5: Tubulin and centromere structures of HeLa cells visualized by
using specific primary antibodies and
secondary antibodies labeled with
PromoFluor 488 and PromoFluor 555.

High quality and ready-to-use


Labeled with PromoFluor dyes
High intensity yields and stable
fluorescent signals

https://www.promokine.info/shop
Product

Size

Catalog Number*

PromoFluor-XXX-Goat Anti-Mouse IgG

1 mg

PK-PFXXX-AK-M1

PromoFluor-XXX-Goat Anti-Rabbit IgG

1mg

PK-PFXXX-AK-R1

PromoFluor-XXX-Goat Anti-Human IgG

1 mg

PK-PFXXX-AK-H1

*XXX- stands for the respective PromoFluor dye (PF405, PF488P, PF488LSS, PF500LSS, PF505 etc). Please see page 146 for details.

8.3.2 PromoFluor Labeled Phalloidin


Applications
Localization and visualization of actin
filaments
Fluorescence microscopy

Features




High quality and ready-to-use


Labeled with the superior PromoFluor
dyes (see page 146)
Yields highly intensive and stable
fluorescent signals

Phalloidin binds very selectively to actin


(much more tightly to actin filaments
than to actin monomers). In combination with conjugated PromoFluor dyes,
phalloidin is enormously useful in localizing actin filaments in living or fixed cells
as well as for visualizing individual actin
filaments in vitro. Ready-to-use phalloidin conjugates of all PromoFluor dyes
are available (10 nmol, 300 units).
Fig. 6: Phalloidin and centromere
structures of HeLa cells stained using a
PromoFluor 647-Phalloidin conjugate
and primary anti-centromer antibodies
in combination with secondary antibodies labeled with PromoFluor 488.

https://www.promokine.info/shop
Product
PromoFluor-XXX, phalloidin conjugate

Size*

Catalog Number**

10 nmol

PK-PFXXX-7-01

*10 nmol are sufficient for staining approximately 300 microscopic slides.
**XXX- stands for the respective PromoFluor dye (PF405, PF488P, PF488LSS, PF500LSS, PF505 etc). Please see page 146 for details.

8 Fluorescent Labeling

151

Applications

Features

properties with regard to signal intensity,


water solubility, and stability. In addition,
the low molecular weight of the dyes
results in minimal steric hindrance and
unproblematic incorporation into DNA
using conventional enzymatic techniques like PCR/RT-PCR. AminoallyldUTP can be incorporated into DNA by
Taq polymerase, Klenow fragment, and
reverse transcriptases. All PromoFluor labeled nucleotides are delivered as 1 mM
ready-to-use aqueous solutions.

Fluorescent labeling of nucleic acids


Fluorescent in situ hybridization
(FISH)
Multicolor FISH (chromosome
painting)
Microarrays

High quality and ready-to-use


Labeled with superior PromoFluor
dyes (see page 146)
High intensity yields and stable
fluorescent signals
Minimal steric hindrance
PromoKine provides aminoallyl-dUTP
(deoxyuridine-triphosphate) conjugates
for all PromoFluor dyes (see page 146).
The labeled nucleotides offer excellent

Fig 7: 2-color labeling of chromosomes


1 and 8 using dUTPs conjugated to
PromoFluor 505 and PromoFluor 555.
Picture kindly provided by Applied
Spectral Imaging.

Fig 8: Fluorescence laser scan of a microarray slide using PromoFluor dyes. Different
matching oligonucleotides, negative controls, and reference oligonucleotides were
spotted in 25 replicates and at 4 different concentrations to epoxysilane coated slides
and hybridized with PromoFluor labeled samples. Picture kindly provided by the
Functional Genomics Center Zurich, Switzerland.
https://www.promokine.info/shop

Product
PromoFluor-XXX-aadUTP

Size

Catalog Number*

100 l

PK-PFXXX-8-100

PromoFluor-430-dUTP (NT)

10 l
50 l

PK-PF430-DUTP-10N
PK-PF430-DUTP-50N

PromoFluor-430-dUTP (PCR)

10 l
50 l

PK-PF430-DUTP-10P
PK-PF430-DUTP-50P

PromoFluor-500-dUTP (NT)

10 l
50 l

PK-PF500-DUTP-10N
PK-PF500-DUTP-50N

PromoFluor-500-dUTP (PCR)

10 l
50 l

PK-PF500-DUTP-10P
PK-PF500-DUTP-50P

PromoFluor-550-dUTP (NT)

10 l
50 l

PK-PF550-DUTP-10N
PK-PF550-DUTP-50N

PromoFluor-550-dUTP (PCR)

10 l
50 l

PK-PF550-DUTP-10P
PK-PF550-DUTP-50P

PromoFluor-594-dUTP (NT)

10 l
50 l

PK-PF594-DUTP-10N
PK-PF594-DUTP-50N

PromoFluor-594-dUTP (PCR)

10 l
50 l

PK-PF594-DUTP-10P
PK-PF594-DUTP-50P

PromoFluor-640-dUTP (NT)

10 l
50 l

PK-PF640-DUTP-10N
PK-PF640-DUTP-50N

PromoFluor-640-dUTP (PCR)

10 l
50 l

PK-PF640-DUTP-10P
PK-PF640-DUTP-50P

TexasRed-dUTP (PCR)

10 l
50 l

PK-PFTXR-DUTP-10P
PK-PFTXR-DUTP-50P

Biotin-dATP (PCR)

10 l (1 mM)
50 l (1 mM)

PK-CA-BIO-DATP-10P
PK-CA-BIO-DATP-50P

Biotin-dCTP (PCR)

200 l (1 mM)
1 ml (1 mM)

PK-CA-BIO-DCTP-200P
PK-CA-BIO-DCTP-1000P

Biotin-dUTP (PCR)

200 l (1 mM)
1 ml (1 mM)

PK-CA-BIO-DUTP-200P
PK-CA-BIO-DUTP-1000P

* XXX- stands for the respective PromoFluor dye (PF-405, PF-488P, PF-488LSS, PF-500LSS, PF-505 etc). Please see page 146 for details.

Fluorescent
Labeling

8.3.3 PromoFluor Labeled Nucleotides

152

8 Fluorescent Labeling

8.3.4 PromoFluor Labeled Biotin and Streptavidin


Applications
Fluorescence microscopy
Flow cytometry
Fluorescent in situ hybridization (FISH)
Fluorescence correlation spectroscopy
(FCS)

Fluorescent
Labeling

Features




High quality and ready-to-use


Labeled with superior PromoFluor
dyes (see page 146)
High intensity yields and stable
fluorescent signals

Streptavidin has a very strong affinity


for biotin with a dissociation constant
of approximately 10-15 mol/l. This is

the highest known affinity between any


protein and its ligand. Extensive chemical
modifications have little effect on this affinity. Streptavidin is a tetrameric 53 kDa
protein. Since it lacks any carbohydrate
modification and has a near-neutral pH,
it has the advantage of much lower nonspecific binding than avidin. On the other hand, streptavidin is less watersoluble
than avidin.
Ready-to-use streptavidin and biotin
conjugates of all PromoFluor dyes (see
page 146) are available. The streptavidin conjugates are affinity-purified and
dissolved in 100 mM PBS (pH 7.5) at a
concentration of 1 mg/ml. The biotin
conjugates are affinity-purified and lyophilized. Each conjugate comes with a
batch-specific absorption spectrum.

Fig. 9: Staining of plasma membranebound neurokinin-1 receptor (red) in a


U2OS cell using a PromoFluor 547-conjugate. Picture kindly provided by Covalys Biosciences AG, Switzerland.

https://www.promokine.info/shop
Product

Size

PromoFluor-XXX, streptavidin conjugate


PromoFluor-XXX, biotin conjugate

Catalog Number*

1 mg in 1 ml PBS

PK-PFXXX-5-01

1 mg

PK-PFXXX-6-01

Note: Please see our website www.promokine.info for an up-to-date list of available fluorescent labeled biomolecules.

*XXX- stands for the respective PromoFluor dye (PF-405, PF-488P, PF-488LSS, PF-500LSS, PF-505 etc). Please see page 146 for details.

8.4 Phycobiliprotein Dyes


Phycobiliproteins are water-soluble
proteins with covalently bound chromophores present in cyanobacteria
and certain algae that capture light energy during photosynthesis. The major
phycobiliproteins are R-Phycoerythrin
(R-PE), B-Phycoerythrin (B-PE), and Allophycocyanin (APC).

as large Stokes shifts. The fluorescence


yield is equivalent to at least 30 unquenched fluorescein or 100 rhodamine
molecules at comparable wavelengths
(on a molar basis), and fluorescence is
not quenched by external agents since it
is protected by covalent binding to the
protein backbone.

Phycobiliproteins can be used as fluorescent dyes to label biomolecules to obtain


specific probes or markers for highly sensitive cell and molecular biological applications such as immunofluorescence
microscopy, flow cytometry, microarrays,
FISH, enzyme kinetic studies, etc. They
show optimal quantum yields (up to
98%) and extinction coefficients as well

PromoKines phycobiliproteins can easily


be linked to antibodies. The sensitivity
of antibodies conjugated with R-PE and
B-PE is usually five to ten times greater
than that of the corresponding fluorescein conjugate.
PromoKine offers R-PE, B-PE, and APC
for labeling of biomolecules such as pro-

teins, antibodies, and nucleic acids. They


show strong light absorption, high fluorescence yields, excellent stability and
high fluorescence over a broad pH range
and are compatible with common excitation sources and filter sets.
Please see www.promokine.info/products/fluorescent-labeling for more information and pricing.

8 Fluorescent Labeling

153

Applications
Mounting medium for fluorescence
microscopy

Features



Significantly increased photostability


Compatible with many common dyes
Conserves cell preparations
Convenient packaging

Irreversible photobleaching processes


lead to a loss of fluorescent signal sensitivity particularly when high intensity excitation light is used for a longer duration
and the target molecules in samples are
in low abundance. PromoFluor Antifade
Reagent significantly increases the photostability of fluorochromes in fixed cells,
fixed tissues, and cell-free preparations.
It is compatible with many common
dyes and dye complexes resulting in prolonged signals and improved image con-

trast especially in multicolor applications.


Moreover, it gently solidifies after the attachment of the cover slide, permanently
conserving the cell preparation and fixing the coverslip. PromoFluor Anitfade
Reagent makes slide immobilization using e.g. nail polish unnecessary. Samples
can be saved for months after mounting,
retaining fluorescence during prolonged
storage.
PromoFluor Antifade Reagent comes
premixed in a convenient dropper bottle.

Fig. 10: Immunofluorescence imaging


of a human keratinocyte stained with
FITC-labeled antibodies without (top)
and with (bottom) PromoFluor Antifade
Reagent at different excitation intervals
(5, 10, 15, 20, 25 seconds).

https://www.promokine.info/shop
Product

Product Description

Size

Catalog Number

PromoFluor Antifade Reagent

Mounting Medium for Fluorescence


Microscopy

8 ml

PK-PF-AFR1

Fluorescent
Labeling

8.5 PromoFluor Antifade Reagent

Cell Transfection
The ability to introduce foreign nucleic acids into eukaryotic
cells has become a powerful tool in studying and controlling
gene expression. Cloned genes can be transfected into cells for
biochemical characterization, mutational analyses, investigating
gene regulatory elements, and producing specific proteins. Cell
transfection promotes the rapid advancement of our knowledge
of genetic regulation and protein function.

156 Content Chapter 9

3
9

Cell Transfection
9.1 PromoFectin Transfection Reagents . . . . . 157
9.2 Magnet Assisted Transfection (MATra) . . . 161
9.3 Transfection Accessories . . . . . . . . . . . . . . 165
9.3.1 GeneBooster . . . . . . . . . . . . . . . . . . . . . . . . 165
9.3.2

9.4

Cell Clean . . . . . . . . . . . . . . . . . . . . . . . . . . 165

Reporter, Expression and Fusion Vectors . . 166

9.4.1

pPK-CMV Reporter Vectors . . . . . . . . . . . . 166

9.4.2

pPK-CMV Expression Vectors . . . . . . . . . . . 166

9.4.3

pPK-CMV Fusion Vectors . . . . . . . . . . . . . . 166

9 Cell Transfection

157

9.1 PromoFectin Transfection Reagents


PromoFectin

PromoFectin is a series of reagents for


highly efficient and reproducible transfection of a variety of adherent and
non-adherent cells that can be used
to transfect nucleic acids or proteins.
PromoFectin comes either as a broadrange reagent or as specialized reagents
for specific cell types and biomolecules.

Efficient transfection of many cell


types including primary cells
Suited for plasmids, oligonucleotides,
siRNA, and polypeptides
For stable and transient transfections

Features
Superior transfection efficiency
Fast procedure and easy handling
without medium change
Compatible with serum and
antibiotics
Good cell survival and viability after
transfection

The standard broad-range PromoFectin


is a non-liposomal transfection reagent
based on a water-soluble, cationic polymer. It is free of animal-derived components and shows very low cytotoxicity.
PromoFectin ensures highly effective
delivery of plasmids, oligonucleotides,
and siRNA into a variety of adherent

Cytoplasm

Nucleus

Nucleic acid

PromoFectin

Cationic
complexes

Endocytosis

Endosome

Proton
sponge

Nucleic acid
in 150 mM NaCl
solution

Incubate
30 minutes at RT
PromoFectin

Incubate 24-28 h.
Then, measure
protein expression.

and non-adherent cell types with a high


degree of reproducibility. Since its development, PromoFectin has been used
successfully with many problematic cell
lines and sensitive primary cells.

Cell Transfection

Applications

Fig. 1: Primary fibroblasts (NHDF, see


page 27) transfected with the GFP
reporter vector pPK-CMV-R4 (see page
166) using PromoFectin.

Fig. 2: PromoFectin assembles nucleic


acids into compact structures that bind
to the cell surface and are delivered
into the cell by endocytosis. In the
endosome, PromoFectin acts as a proton sponge, buffering the endosomal
pH that leads to pH inhibition of endosomal nucleases. Then, continuous
proton influx leads to osmotic swelling
and rupture of the endosome - allowing for almost complete release of the
nucleic acids into the cytosol. Moreover, PromoFectin also favours DNA
trafficking to the cell nucleus a property that is particularly useful for transfecting slow-dividing or resting cells.

Fig. 3: The PromoFectin transfection procedure is fast and easy. Add


PromoFectin to the nucleic acid solution, mix, incubate for 15-30 minutes, add growth medium (which can
contain serum and antibiotics), and
pipette the complexes onto the cells.
The cells are then incubated for further
analysis. No post-transfection removal
of complexes or medium change/addition is necessary.

158

9 Cell Transfection

Cell Transfection

Fig. 4: Comparison of transfection efficiencies for PromoFectin


and three other common transfection reagents recommended
for primary cells. Primary human fibroblasts (NHDF, see page
27) cultured in 24-well plates were transfected with a luciferase reporter plasmid*. The appropriate protocols recommended by the manufacturers were used for transfection
with PromoFectin and the competing reagents. All transfections were performed in triplicate (values normalized to
1 mg protein).

* See page 166 for reporter plasmids and page 107 for reporter assays.

PromoFectin-HUVEC

PromoFectin-Hepatocyte

PromoFectin-Macrophage

PromoFectin-HUVEC is a non-liposomal
PromoFectin derivative optimized for
the transfection of primary endothelial
cells such as HUVEC1 and HDMEC2. It
shows very little cytotoxicity and enables
very high transfection rates also with
fragile, sensitive cell types. Once inside
the cell, PromoFectins unique proton
sponge property provides an excellent
escape mechanism for DNA particles to
the cytoplasm.

PromoFectin-Hepatocyte is a ligandconjugated, non-liposomal derivative of


PromoFectin specialized for hepatocyte
transfection (e.g., primary human and
murine hepatocytes, hepatocyte cell
lines, and hepatocarcinoma cells). The
reagent/nucleic acid complexes bind
specifically to surface receptors on the
target cells leading to extremely high
transfection rates.

PromoFectin-Macrophage was developed for optimal transfection of the


known hard-to-transfect macrophages
(e.g. primary human macrophages
obtained from blood monocytes) and
macrophage-derived cell lines such as
RAW 264.7. PromoFectin-Macrophage
is a mannose-bearing variant of the
standard PromoFectin reagent. It binds
to cells expressing mannose-specific
membrane receptors - such as macrophages, monocytes and dendritic cells leading to enhanced internalization of
the DNA complexes.

Fig. 5: Primary human endothelial cells


(HUVECs) transfected with the reporter
vector pPK-CMV-R4 (see page 166)
using PromoFectin-HUVEC. Nuclei counterstained with DAPI.

Fig. 6: Primary human hepatocytes


transfected with the reporter vector
pPK-CMV-R4 (see page 166) using PromoFectin-Hepatocyte.

Fig. 7: Human monocyte-derived macrophages were transfected with a galactosidase-expressing plasmid after 7 days
of culture in the presence of 100 U/ml
GM-CSF and serum-containing medium .

1
2

Human Umbilical Vein Endothelial Cells (see page 13)


Human Dermal Microvascular Endothelial Cells (see page 17)

9 Cell Transfection

159

Cell Transfection

Fig. 8: Comparison of transfection efficiencies for PromoFectinHUVEC, PromoFectin, and three other common transfection reagents recommended for primary cells. Primary
HUVEC (see page 13) cultured in 24-well plates were transfected with a luciferase reporter plasmid*. The appropriate
protocols recommended by the manufacturers were used for
transfection with PromoFectin and the competing reagents.
All transfections were performed in triplicate (values normalized to 1 mg protein).

* See page 166 for reporter plasmids and page 107 for reporter assays.

PromoFectin-Neuron
PromoFectin-Neuron is a cationic polymer-based transfection reagent created
for the transfection of primary neuronal
cells, glial cells, and neuronal cell lines.
Cells take up the polymer/DNA complex
through endocytosis and the polymer is
biodegraded into non-toxic molecules.
Compared to viral methods, PromoFectinNeuron allows less complicated and
faster nucleic acid delivery with high
transfection rates.

PromoFectin-Insect
PromoFectin-Insect is a unique, lipsomebased transfection reagent optimized
for nucleic acid delivery into insect cells,
especially those derived from Spodoptera frugiperda (e.g. Sf9 or Sf21). It
outperforms other insect cell transfec-

Fig. 9: Primary neurons transfected with


the reporter vector pPK-CMV-R4 (see
page 166) using PromoFectin-Neuron.

tion reagents in comparison studies and


requires less DNA. PromoFectin-Insect
demonstrates broad vector delivery capability, excellent reproducibility, and
high expression levels for small- or largescale protein production.

a wide variety of cell types, including


problematic cell lines and primary cells. It
enables optimal gene silencing with low
cytoxicity.

PromoFectin-CellLine

PromoFectin-Polypeptide is a novel liposomal reagent created for the highly


efficient and fast delivery of proteins and
polypeptides (e.g. blocking peptides and
labeled antibodies) into a wide variety of
cells. It captures protein molecules and
transports them into the cytosol of living
cells while maintaining the proteins
structure and functionality. This allows
researchers to study intracellular protein
functions without the need for DNA
cloning and DNA transfection.
PromoFectin-Polypeptide is also available
in pre-coated single-use tubes, providing
additional convenience and speed.

PromoFectin-CellLine is a cationic lipidbased reagent developed to obtain optimal transfection results with several
widely used cell lines (CHO, HeLa, COS,
293, NIH-3T3) at low costs. Its formulation and the provided protocol allow fast
and convenient cell transfection.

PromoFectin-siRNA
PromoFectin-siRNA is a cationic lipidbased transfection reagent designed for
the highly efficient delivery of siRNA into

PromoFectin-Polypeptide

Fig. 10: Left: NIH-3T3 cells transfected with the GFP reporter plasmid pPK-CMV-R4
(see page 166) using PromoFectin-CellLine. Right: CHO cells transfected with the
GFP reporter plasmid pPK-CMV-R4 (see page 166) using PromoFectin-CellLine.

160

9 Cell Transfection

Cell Transfection

n Some cell types successfully transfected with PromoFectin

Adipocyte cells

Primary human pre-adipocytes

Endothelial-like cells

BBMC; BAEC; HLMECa, HUVECa, H-MVEC, primary human Lung Lymphatic Endothelial
cells, primary HUVECa, primary HDMECa

Epithelial-like cells

2CSFMEo, 3LL, 56FHTE8o, 6CSFMEo, 9HTEo-, A549, AsPC-1, BxPC-3, CaCo2, Ca Ski,
CFNPE90- CF, CFPEo, CFSMEo, CHO, D 407, DHD Pro.b, HeLa, Huh-7D12, IGROV1,
MCF-7, MDA-MB435S, MDCK, MLE-12, MLE-15, MRC-5, PGHAM-1, SAOS-2, SiHa,
SKOV3, U-2 OS, HEK293, HEK293FT

Fibroblast-like cells

BHK-21, COS-7, CV-1, L929, NIH 3T3, MEF, primary human skin fibroblasts (NHDF)

Hepatocyte-like cells

BNL, CL 2b, Hepa 1-6, HepG2b, Huh-7b, M-PAC, primary human/mouse/rat hepatocytesb

Keratinocytes

Primary human keratinocytes

Pancreatic carcinoma cells

AR42J, BxPC-3, MIAPaCa-2, PanC-1, PGHAM-1

Leukemia cells / lymphoblasts

Daudi, Jurkat, K562, Molt 4

Melanoma cells / melanocytes

B16-F10, primary human epidermal melanocytese

Myotubes / myoblasts / muscle cells

C2C12

Neuronal cells

CLBPEC, Neuro2A, PC12, SH-SY5Y, SHEP, SK-N-MC, F11, NG 108-15d, primary chicken
embryonic neurons, primary rat and mouse cortical neuronsd, primary rat hippocampal
neuronsd, primary rat cerebellar granular neuronsd, primary rat striatal neuronsd

Chondrocytes

HCS-2/8

Glioblastoma

U87

Blood cells

Human macrophagesc, monocyte-derived macrophagesc, RAW 264.7c, THP-1c

Mesenchymal stem cell

STRO-1+ DaMSCs cells

PromoFectin-HUVEC,

PromoFectin-Hepatocyte,

PromoFectin-Macrophage,

PromoFectin-Neuron,

Promofectin-siRNA

Please contact us or see www.promokine.info for information about new cell types that have been successfully transfected
using PromoFectin.
https://www.promokine.info/shop
Product

Size

Catalog Number

PromoFectin

0.1 ml
0.5 ml
1 ml

PK-CT-2000-10
PK-CT-2000-50
PK-CT-2000-100

PromoFectin-HUVEC

0.1 ml
0.5 ml

PK-CT-2000-HUV-10
PK-CT-2000-HUV-50

PromoFectin-Hepatocyte

0.1 ml
0.5 ml

PK-CT-2000-HEP-10
PK-CT-2000-HEP-50

PromoFectin-Macrophage

0.1 ml
0.5 ml

PK-CT-2000-MAC-10
PK-CT-2000-MAC-50

0.15 ml
0.75 ml
5 x 0.75 ml

PK-CT-2000-NEU-015
PK-CT-2000-NEU-075
PK-CT-2000-NEU-375

0.5 ml
1 ml

PK-CT-2000-InC-50
PK-CT-2000-InC-100

0.75 ml
1.5 ml

PK-CT-2000-CL-075
PK-CT-2000-CL-150

0.2 ml
0.75 ml
5 x 0.75 ml

PK-CT-2000-RNA-50
PK-CT-2000-RNA-200
PK-CT-2000-RNA-1000

24 reactions
24 Single Use Vials
96 reactions
96 Single Use Vials

PK-CT-2000-POL-24
PK-CT-2000-POL-24Q
PK-CT-2000-POL-96
PK-CT-2000-POL-96Q

PromoFectin-Neuron

PromoFectin-Insect
PromoFectin-CellLine
PromoFectin-siRNA

PromoFectin-Polypeptide

9 Cell Transfection

161

9.2 Magnet Assisted Transfection (MATra)


Magnet Assisted Transfection

Transfection of adherent and suspen-


sion cells including primary cells
Suited for plasmids, oligonucleotides,
siRNA, and adenovirus/retrovirus
transfection
For stable and transient transfections
Also suited for high-thoughput
transfection

Adherent Cells

Suspension Cells

Magnet Assisted Lipofection


Adherent Cells

Use MATra-S
Immobilizer to make
the cells adherent.

Suspension Cells
Use MATra-S
Immobilizer to make
the cells adherent.

MATra-A
or MATra-si
Reagent

MA Lipofection Enhancer and lipofection


reagent of choice

Transfected
Cells

Transfected
Cells

Features








Easy, fast, and reproducible procedure


Functional with and without serum
and antibiotics
Very high transfection rates also with
many hard-to-transfect cell types
Up to several thousand fold increased
transgene expression
Very low amounts of nucleic acid
necessary for transfection

Magnet Assisted Transfection (MATra) is


a very fast, easy-to-handle, and highly
efficient technology for transfecting cells
in culture. It can be used for cells from a
broad variety of species. The major components of MATra are coated magnetic
nanoparticles, which effectively bind either nucleic acids or liposomes. Using a
special magnet plate, the nanoparticles
with the bound nucleic acids or liposomes are rapidly drawn towards the
cells and are deposited at a high concentration on the outer cell membrane.
Afterwards, the cells endocytose the
particles, which leads to a high transfection rate even with problematic cells.

MATra
nanoparticles

The MATra technology is distributed for IBA GmbH, Germany. For research use only!

Two approaches are possible:


Magnet Assisted Transfection using
MATra-A or MATra-siRNA Reagent.
Magnet Assisted Lipofection using
the MA Lipofection Enhancer and a
lipofection reagent of choice.
Both techniques can be used with adherent cells as well as with suspension cells.
For suspension cells, the cells have to be
immobilized at the bottom of the wells
to make use of the magnetic field. This
can be achieved by either temporarily
immobilizing the cells with MATra-S Immobilizer or by using modified culture
vessels. MATra is also suited for highthroughput transfection assays using
adapted protocols.

DNA

DNA + nanoparticle
complex

Magnet
Magnet Plate

Adherent cells

Fig. 11: Magnet Assisted Transfection (MATra) principle.

MATra-A Reagent
The MATra-A Reagent contains magnetic nanoparticles with an innovative
polyelectrolyte coating, which effectively
bind nucleic acids (e.g. plasmid DNA or
oligonucleotides) by means of electrostatic interactions. Using this technique,
nucleic acids are initially mixed with specifically coated magnetic nanoparticles.
The magnetic particles loaded with the
nucleic acids are then added to cells in
culture and a strong magnetic force is
applied beneath the cells. Exploiting this
strong magnetic field, the full nucleic
acid dose is rapidly drawn towards the
target cells on the bottom of the multiwell plate or cell culture flask. This results
in very close contact between the cell
membrane and the highly concentrated
nucleic acid loaded magnetic complexes
allowing optimal nucleic acid delivery.
MATra allows efficient transfection
without disturbing the cell membrane
architecture or leaving a hole in the cell
membrane as with other transfection
technologies. The transfection rate consequently is clearly increased - also with
difficult-to-transfect cells such as primary
cells. In addition, the amounts of nucleic
acids required for successful transfection
can be significantly reduced, which in
turn further reduces cytotoxicity.

Cell Transfection

Applications

162

9 Cell Transfection

GFP fluorescence

Overlay

Fig. 12: Mouse fibrosarcoma cells transfected with GFP plasmid using MATra (Data kindly provided for IBA GmbH by Dr. Lutz
Thon and Dr. Dieter Adam, Institute for Immunology, University Schleswig-Holstein, Kiel, Germany).

MATra-siRNA Reagent
siRNAs are small interfering/inhibitory
sequence-specific RNA molecules that
bind to a complementary sequence
of a target mRNA, block translation of
that target mRNA, and initiate its degradation. Consequently, synthesis of
the corresponding protein of interest is
prevented and the specific gene function
is knocked-out. This process is termed
RNA interference or gene-silencing and
it is used to elucidate gene functions.

Specifically designed small inhibitory


RNA oligonucleotides (siRNAs) allow
transient inhibition of gene expression in
vitro and in vivo.
MATra-siRNA contains specifically coated magnetic nanoparticles, which are
optimized to effectively bind and stabilize siRNA. It provides optimal delivery
of siRNA into many different cell types
enabling efficient and prolonged genesilencing.

Fig. 13: Human endometrial stromal cells


transfected with fluorescein-labeled
siRNA using MATra-siRNA Reagent
(Data kindly provided for IBA GmbH by
Dr. Birgit Gellersen, Endokrinologikum
Hamburg, Germany).

%GFP positive cells


50

40

30

20

* See page 166 for reporter plasmids.

Reagent LF

Reagent S18

Reagent FG6

10

PromoFectin-HUVEC

Fig. 14: Comparison of transfection efficiencies of MATra,


PromoFectin-HUVEC, and three other common transfection
reagents recommended for primary cells. Primary human
dermal microvascular endothelial cells (HDMEC, see page
17) cultured in 24-well plates were transfected with a GFP
reporter plasmid*. The appropriate protocols recommended
by the manufacturers were used for transfection with MATra,
PromoFectin, and the competing reagents. All transfections
were performed in triplicate.

MATra

Cell Transfection

Phase contrast

9 Cell Transfection

The MA Lipofection Enhancer contains


coated magnetic nanoparticles which
bind liposomes and any polycationic
transfection reagents (e.g. PromoFectin)
which in turn bind nucleic acids (e.g.
plasmid DNA, oligonucleotides, siRNA).
Using the MA Lipofection Enhancer, cells
are transfected more efficiently than
with liposomal or polycationic transfection reagents alone.

MATra-S Immobilizer
Suspension cells have to be made adherent first to perform Magnet Assisted

Transfection (as well as many other conventional transfection procedures). The


MATra-S Immobilizer contains coated
magnetic nanoparticles which bind suspension cells. Using a special magnet
plate, the magnetic nanoparticles with
the bound cells are rapidly drawn towards the bottom of the cell culture vessel where they are immobilized. By combining the MATra-S Immobilizer with the
other MATra reagents, suspension cells
can be transfected conveniently.
See www.promokine.info for a listing of more than 100 cell types
successfully transfected using Magnet Assisted Transfection and Magnet
Assisted Lipofection.

Liposome

Cell membrane

Magnetic nanoparticles

Fig. 15: Principle of Magnet Assisted


Lipofection. The MA Lipofection
Enhancer can be combined with any
commercially available transfection
reagent based on polycations or lipids.

Response (expression level)


200

150

MATra

100

Fig. 16: Magnet Assisted Transfection and lipofection in comparison. Luciferase expression was assayed after 24 hours.
With MATra reagents, low vector doses are sufficient to obtain good results.

50
2.

tion
genera

fection reagents

0.05

0.1

%GFP positive cells


10
8
6
4

Reagent LF

Reagent LF +
MA Lipopfection Enhancer

IBAfect

IBAfect +
MA Lipopfection
Enhancer
* See page 166 for reporter plasmids.

nts

reage

1. generation lipo

Dose (g DNA)

Fig. 17: Comparison of transfection efficiencies of two lipofection reagents with and without MA Lipofection Enhancer. Primary human dermal microvascular endothelial
cells (HDMEC, see page 17) cultured in 24-well plates were
transfected with a GFP reporter plasmid*. The appropriate
protocols recommended by the manufacturers were used for
transfection. All transfections were performed in triplicate.
IBAfect liposomal transfection reagent is also available at
PromoKine. Please visit www.promokine.info for more information.

ction

lipofe

0.15

Cell Transfection

MA Lipofection Enhancer

163

164

9 Cell Transfection

Magnet Plates

24 Magnet Bar Plate (8 x 12 cm):


optimized for 24-well plates.
96 Magnet Bar Plate (8 x 12 cm):
optimized for 96-well plates
Also available: Magnet Bar Plate Sets.

Cell Transfection

To perform Magnet Assisted Transfection, specially designed neodymium


magnet plates are necessary. PromoKine
offers them in different sizes, optimized
for different cell culture vessels.

Universal Magnet Plate (8 x 13 cm):


suitable for 6-well, 12-well, 24-well,
48-well, or 96-well plates, T75 flasks, 60
mm dishes etc.
Universal Magnet Plate (26 x 26 cm):
suitable for 6-well, 12-well, 24-well,
48-well, or 96-well plates, T75 flasks,
60 mm dishes, 100 mm dishes etc.

Fig. 18: Universal magnet plate (8 x 13 cm) with collection of different culture plates
and MATra reagents.

Fig. 19: 96 magnet bar plate (8 x 12 cm)


with 96-well culture plate.

https://www.promokine.info/shop
Product

Size

Catalog Number

MATra-A Reagent

0.05 ml
0.2 ml
1 ml

PK-CT-2001-005
PK-CT-2001-020
PK-CT-2001-100

MATra-siRNA Reagent

0.05 ml
0.2 ml
1 ml

PK-CT-2021-005
PK-CT-2021-020
PK-CT-2021-100

MA Lipofection Enhancer

0.05 ml
0.2 ml
1 ml

PK-CT-2003-005
PK-CT-2003-020
PK-CT-2003-100

MATra-S Immobilizer

0.05 ml
0.2 ml
1 ml

PK-CT-2002-005
PK-CT-2002-020
PK-CT-2002-100

Universal Magnet Plate, 8 x 13 cm plate


Universal Magnet Plate, 26 x 26 cm plate
24 Magnet Bar Plate, 8 x 12 cm plate
96 Magnet Bar Plate, 8 x 12 cm plate

PK-CT-2011-000
PK-CT-2012-000
PK-CT-2006-000
PK-CT-2004-000

Four 24 Magnet Bar Plates


Four 96 Magnet Bar Plates
Two 24 and two 96 Magnet Bar Plates

PK-CT-2008-000
PK-CT-2009-000
PK-CT-2010-000

Magnet Plate

Set of four Magnet Bar Plates

Note: Please contact us to request a test offer for MATra.

9 Cell Transfection

165

9.3 Transfection Accessories

Applications
Enhancement of gene expression in
transfected cells

Fast and easy protocol


Optimized conditions for popular
cell lines
Efficient for both transient and
stable transfections

Features




Up to 12-fold increased gene


expression levels in diverse cell
lines after transfection
Compatible with all commonly used
cell transfection reagents

The GeneBooster Reagents are chemical cocktails designed to significantly


enhance gene expression levels in transfected cells. Three unique formulations
have been developed for different cell
types. Simply add GeneBooster reagent

to the culture medium four hours after


transfection and an increase in expression levels up to 12-fold can be
achieved. The reagents can be used in
combination with all commercially available DNA transfection reagents (including all PromoFectin Reagents). One vial
of GeneBooster Reagent is sufficient for
150 transfections using 1 ml transfection
volume.
The three GeneBooster Reagents are
also available in a kit.

Fig. 20: K562, HeLa-S3, MCF7, and P19 cells were transfected with the beta-Gal reporter plasmid pPK-CMV-R1 (see page 166).
After 4 hours, the appropriate GeneBooster reagent was added to the cells which were stained 48 hours post transfection using a
beta-Gal Staining Kit (see page 107) and examined under a microscope.

9.3.2 Cell Clean


Applications
Highly efficient cleanup of
transfected cells

Features
Efficient removal of extracellular lipid/
DNA complexes after cell transfection
Non-toxic to cells
Fast and easy procedure
Better downstream results post
transfection
CellClean is a unique washing buffer that
has been designed to remove all com-

plexes of DNA and cationic lipids that


associate with cell surfaces during transfection. It shows no cytotoxicity and can
distinguish between extracellular and
intracellular plasmid DNA. When CellClean is used as part of the cell washing
procedure, more precise quantitation of
the amount of DNA delivered into cells
can be achieved. Moreover, it improves
downstream results such as DNA stability, cytometry, and gene delivery studies.
Fig. 21: Adherent cells were incubated with cationic lipid/DNA complexes for 4 hours
and washed with PBS (lane 1) or CellClean (lane 2). Cells were lysed, the DNA extracted and analysed by agarose gel electrophoresis. Arrows show extracellular plasmid
DNA that could be removed from cell surfaces when using Cell Clean.

https://www.promokine.info/shop
Product

Size

Catalog Number

GeneBooster Reagent I

3 vials x 1.5 ml each

PK-CT-2020-1

GeneBooster Reagent II

3 vials x 1.5 ml each

PK-CT-2020-2

GeneBooster Reagent III

3 vials x 1.5 ml each

PK-CT-2020-3

GeneBooster Reagent Kit

3 vials x 1.5 ml each

PK-CT-2020-0

100 ml

PK-CT-1000-01

CellClean

Cell Transfection

9.3.1 GeneBooster

166

9 Cell Transfection

Cell Transfection

9.4 Reporter, Expression and Fusion Vectors


Applications

Features

Transient high-level expression of five


popular reporter genes
Constitutive high-level target gene ex pression yielding native or HA-tagged
proteins
Constitutive high-level GFP or Lucifer-
ase fusion protein expression
Monitoring transfection efficiency and
gene expression
Creating GFP or Luciferase expressing
stable cell lines

Optimized CMV promoter provides


high level gene expression
Suited for a wide variety of cell types
for both stable and transient gene ex-
pression
Small vector sizes ensure high transfec-
tion efficiency and high plasmid yield
in E. coli
Convenient multiple cloning site with
most commonly-used restriction sites
for optimized cloning and expression
of target genes

The pPK-CMV Vectors contain a modified human cytomegalovirus (CMV)


based promoter/enhancer sequence that
has been optimized for high level gene
expression. Sequence motives that limit
expression have been removed, while
others that promote high expression
levels have been retained. Consequently,
these vectors yield significantly higher
expression levels than other expression
vectors based on the standard CMV
promoter. In addition, the plasmid backbones are optimized to allow for very
small vector sizes and higher plasmid
yields when amplified in E. coli.

Please check our website at www.promokine.info for details and prices.

9.4.1 pPK-CMV Reporter Vectors


The pPK-CMV Reporter Vectors have
been designed for high level, transient
expression of reporter or target genes in
a wide variety of mammalian cells and
tissues (in vitro or in vivo). The vectors
are available without a reporter gene
to clone and express a gene of interest

(blank vector) as well as with the following reporter genes: -galactosidase


(lacZ), green fluorescent protein (GFP),
luciferase (Luc), chloramphenicol acetyltransferase (CAT), and secreted alkaline
phosphatase (SEAP).

See also page 107/108 for luciferase,


beta-galactosidase and GFP reporter
assays.

Plasmid maps, multiple cloning sites, and


DNA sequences are available on our website.

9.4.2 pPK-CMV Expression Vectors


The pPK-CMV Expression vectors have
been designed for constitutive high-level
transgene expression. Three versions
of the pPK-CMV Expression Vector are
available: pPK-CMV-E1 does not have
an expression tag. It is intended for
expressing recombinant proteins in the

native forms. pPK-CMV-E2 comes with


an N-terminal HA (hemagglutinin) fusion tag. pPK-CMV-E3 comes with a
C-terminal HA-fusion tag. The HA fusion
tags allow easy detection and purification of proteins.

All three Expression Vectors come with a


positive control vector containing a CAT
gene.

multiple cloning sites upstream or downstream of the reporter gene.


Four versions of the fusion vector are
available for convenient and efficient
gene cloning and expression of the target gene fused with a reporter gene:
pPK-CMV-F1 (C-GFP) has a GFP coding sequence with a stop codon inframe
in the MCS. The gene of interest can
therefore be expressed with GFP at the
C-terminus. pPK-CMV-F2 (N-GFP) has
a GFP coding sequence without a stop
codon inframe in the MCS. The gene
of interest can therefore be expressed
with GFP at the N-terminus. pPK-CMVF3 (C-Luc) has a Luciferase coding se-

quence with a stop codon inframe in the


MCS. The gene of interest can therefore
be expressed with Luciferase at the Cterminus.
pPK-CMV-F4 (N-Luc) has a Luciferase
coding sequence without a stop codon
inframe in the MCS. The gene of interest
can therefore be expressed with Luciferase at the N-terminus.

9.4.3 pPK-CMV Fusion Vectors


The pPK-CMV Fusion Vectors have been
designed for constitutive high-level expression of GFP or Luciferase fusion
proteins and efficient selection in both
E. coli (using kanamycin) and mammalian cells (using neomycin). An SV40
polyadenylation signal provides efficient
transcription termination and polyadenylation - thus ensuring stable mRNA. A
pUC origin of replication allows for high
copy numbers in E. coli.
The vectors can be used for either
transient transfection or for generating
stable cell lines expressing only the reporter gene or the fusion target-reporter
gene construct. The vectors contain two

Please check our website at www.promokine.info for details and prices.

See also page 107/108 for luciferase


and GFP reporter assays.

Antibodies and ELISAs


Antibodies have a broad range of applications like the detection of cellular structures as well as the specific identification,
localization, and purification of intracellular and extracellular
biomolecules such as proteins. They are also essential tools in
describing cellular processes and for diagnostic purposes such
as ELISAs. Consequently, they are important for many research
disciplines like apoptosis, signal transduction, neurobiology,
virology, and tumor biology.

10

168 Content Chapter 10

10

Antibodies and ELISAs


10.1 Mono- and Polyclonal Antibodies . . . . . . . 169
10.2 Antigens and Cell Lysates . . . . . . . . . . . . . 172
10.3 Labeled Antibodies and Labeling Kits . . . . 173
10.4 ELISA Kits . . . . . . . . . . . . . . . . . . . . . . . . . 175

10 Antibodies and ELISAs

169

10.1 Mono- and Polyclonal Antibodies


ies, immunofluorescence microscopy,
immunohistochemistry, immunoprecipitation or Westem blotting. The antibodies have been produced in several different hosts (e.g. rabbit, mouse, rat, goat)
and show specific reactivities against
antigens from different organisms (e.g.
human, mouse, rat). For many of our
antibodies, blocking peptides as well as
cell and tissue lysates are available that
can be used for functional studies or as
positive controls (see page 172). Other

primary antibodies are also available as


conjugates with e.g. FITC or biotin.
PromoKine lists several thousand
primary antibodies on its website. Please
visit www.promokine.info/antibodies to
find your specific antibody of interest using our convenient search function.
PromoKine provides a variety of primary
antibodies for many different research
topics:

https://www.promokine.info/shop

n Cell Signaling: Cytokines, Growth Factors, CD Antigens, soluble Receptors, and Ligands
Numerous antibodies against Interleukins (ILs), Interferons (IFNs), Fibroblast
Growth Factors (FGFs), Monocyte
Chemotactic Proteins (MCPs), Macrophage Inflammatory Proteins (MIPs),
Transforming Growth Factors (TGFs),
Tumor Necrosis Factors (TNFs), Vascular Endothelial Growth Factors and

Receptors (VEGFs, VEGFRs), InsulinLike Growth Factors and Binding Proteins (IGFs, IGF-BPs), Platelet-Derived
Growth Factors (PDGFs), CC Chemokine
Receptors (CCRs), Bone Morphogenetic
Proteins (BMPs), Matrix Metalloproteinases (MMPs), Neurotrophins (NTs),
Stromal Cell-Derived Factors (SDFs),

beta-Defensins (BDs), Eotaxins, diverse


CD antigens, WNTs as well as Adiponectin, BAFF, EGF, G-CSF, GM-CSF, M-CSF,
HGF, EPO, Insulin, Leptin, PLGF, Resistin,
RANKL, RANTES, TIMP, TPO and many
more targets.

Please see www.promokine.info/antibodies/cell-signaling


n Signal Transduction
Numerous antibodies against Akt-1,
ATF-6, BAP29 & BAP36, CIKS, CTRP,
DARP, GADPH, Hrk, IKAP, IKKs, IRAKs,
JNK1, MADD, MAPKAP1, MyD88,

Nanos, PAK, PTEN, Raptor, SATB1 &


SATB2, SHP2, Ski, Slug, SOCS1, STAT1,
Sumo, TOR, TRAF, TRPC, TSC, Wnt-10,
XBP-1, XEDAR and many more targets.

Please see www.promokine.info/antibodies/signal-transduction


n Apoptosis
Numerous antibodies against Caspases, Calpains, Death Receptors (DRs),
HDACs and HATs as well as Acinus, AIF,
Annexin V, Apaf-1, APO-1/Fas, ASK1,

Bad, Bax, Bcl-2, Bid, CAD, CARD, CIDE,


Cytochrome c, Daxx, DcR, DFF, DRAK,
DRAM, Fas Ligand, FLASH, FLIP, ICAD,
ILPIP, LAMP, NALP, NOD2, PDCD,

Please see www.promokine.info/antibodies/apoptosis


n p53 Network
Antibodies e.g. against p53, p53DINP1/
SIP, p53R2, PARC, PERP, PID/MTA2,
ChK2, and MTBP.

Please see www.promokine.info/antibodies/p53-network

PHAP, PIST, PUMA, RAIDD, RICK, RIP3,


Smac, SODD, Survivin, TACE, TCCR,
TIGAR, TRAIL, TWEAK, XAF, XIAP, ZIPK
and many more targets.

Antibodies and
ELISAs

PromoKine offers a wide and continuously growing range of mono- and


polyclonal antibodies against a variety
of interesting molecules like cytokines
and growth factors as well as many
other important key targets in immunology, signal transduction, apoptosis,
neurobiology, virology, tumor biology,
autophagy, homeostasis etc. All antibodies are purified and highly active and can
be used for diverse applications such as
ELISAs, flow cytometry, functional stud-

170

10 Antibodies and ELISAs

https://www.promokine.info/shop

n Neurobiology

Antibodies and
ELISAs

Numerous antibodies against APH1,


APP, BACE, BRSK, CAPS, Clusterin/
ApoJ, CDNF, CRMP1, DISC1, FEZ,

GFR-alpha, Grik, KIF5, KLHL1, LGI,


MANF, Neuritin, Neurotrypsin, Neurturin, Nicastrin, NK3R, NPAS3, PEN2,

SAPAP, Slitrk, Syntaphilin, TDP43,


TMP21, TOCA and many other targets.

Please see www.promokine.info/antibodies/neurobiology


n Stem Cell Research
Numerous antibodies against Bad, betaActin, ADAM10, Adiponectin, Akt1/PKB,
Bone Morphogenetic Proteins (BMPs),
CD44, CD90, CD105, CXCR4, DKK,
Dnmt2, Elk-1, Eotaxin, Erk2, FGF-2,

GDF-9, GM-CSF, GRO-beta, HDACs,


IFN-gamma, IKK-alpha, IL-1, IL-3, IL-6,
IL-8, IL-12, IL-13, Lck, LRP-5/6, MCP-1,
MEK1, MIP-1, MIP-2, NFkB, Nanog,
Notch 1, Oct-4, PDGFR, PPAR-gamma,

PTEN, Raf-1, RANTES, SCF, SDF-1,


Smad, Sox, SSEA, Stat, Synapsin, Tau,
TGF-beta, TLR, TRAF6, Tubulin, VEGF,
Vimentin, Wnt and many other targets.

Please see www.promokine.info/antibodies/stem-cell-research


n Cancer Research
Numerous antibodies against BRCA,
Cathepsins, CD26, CD44, CUEDC,
EMAP-2, FAP, Galectin-3, HEC-1,

ICAM-1, IRF7, JMJD, MART-1, Matrix


Metalloproteinases (MMPs), MnSOD,
PARC, PAX6, Podoplanin, PTEN, OCIAD,

SIPA1L, SRC3, TIMP-1, TNF-alpha &


TNF-beta, VCAM-1, VEGF and many
more targets.

Please see www.promokine.info/antibodies/cancer-research


n Virology
Numerous antibodies against HIV and
Chemokine Receptors (e.g. CCRs,
CX3CR1, CXCR4, DC-SIGN, DDX-3,
HIV-I and HIV-II Glycoproteins, TRIM5),
Herpes (e.g. TNFRSF14/HVEM), SARS
and SARS Receptors (e.g. ACE2, SARS E,

SARS M, SARS Spike, SARS-NP), Anthrax


(e.g. Anthrax EF, Anthrax LF, Anthrax
PA, ATR/TEM8), West Nile Virus (e.g.
WNV Core, WNV Env, WNV Matrix),
Influenza (e.g. Influenza-A and Influenza-B), Avian Influenza (e.g. AFHA, H5N1

Neuramidase and Hemagglutinin, H5N1


NS1/NS2), Hepatitis (e.g. HCVNS3,
HDV, Hepatitis B), Epstein-Barr and
Dengue Virus, (e.g. EBV, Dengue type 2)
and virus-related targets (e.g. Rift Valley
Fever Virus, UNG1, UNG2).

Please see www.promokine.info/antibodies/virology


n Toll-Like Receptor Related
Numerous antibodies against Toll-Like
Receptors (TLRs, TLR1-TLR11) as well

as BTK, ECSIT, MD-1 & MD-2, RIG-1,


SARM, SIGIRR, ST2, TAB1, TIRAP, TIRP,

TOLLIP, TRIAD3A, TRIF, UBC13 and


many other targets.

Please see www.promokine.info/antibodies/toll-like-receptor-related


n Homeostasis
Numerous antibodies against ATM,
beta-Actin, Tumor Endothelial Markers
(TEMs), BRCC, CLIP170, CSN8, EVER,

LIMP2, LSD1, MORF4, Nephrin, op18,


PTK7, RAP80, RGP1, RTP801, SCO, Slc,
STEAP, TNPO3, TP1, TRIM25, VPS53

Please see www.promokine.info/antibodies/homeostasis

and many more targets.

10 Antibodies and ELISAs

171

https://www.promokine.info/shop

n Kinases & Phosphatases


IKK, JIK, JKK, Lck, LIMK, LOK, LOX-1,
MAPKAPK-2, Mek, MST-1, NIK, PAK,
PDK1, PKA, PKC, PTP-1B, Raf1, RIPK3,

Please see www.promokine.info/antibodies/kinases-and-phosphatases


n Phospho-specific Antibodies
Numerous antibodies against phosphorylated AFX, Akt, ATF2, Bad, BRCA1,
c-Jun, c-Myc, Elk-1, Erk1/2, FAK, GSK-3,

IRS, JKK, Lck, MAPKAPK-2, Mek1/2,


MSK1, PKC, Raf, Stat, Tau, TrkA, VASP
and many other targets.

Please see www.promokine.info/antibodies/phospho-specific-antibodies


n Immunology
Many antibodies e.g. against ADAP,
Blimp-1, Carabin, CRTH2, IFN-beta,

IL-33, MICA, ORAI, STIM2, TSLP and


TSLP Receptor.

Please see www.promokine.info/antibodies/immunology


n Innate Immunity
Many antibodies e.g. against Cathelicidin, IPR1, LGP2, MDA5, SLPI, VISA,

Unc93b, and ZBP1.

Please see www.promokine.info/antibodies/innate-immunity


n Autophagy
Antibodies e.g. against Ambra1, APG5
& APG7, Apo-1/Fas, ATG, Bad, Bax,

Bcl-2, Bid, Bnip3L, DRAM, IRGM, IRS-1,


LAMP, Mek1, PIST, Presenilin, and PTEN.

Please see www.promokine.info/antibodies/autophagy


n Subcellular Markers
Antibodies e.g. against Apaf-1, Bad, Bax,
Bcl-6, Bcl-xL, Bid, CREB, DKK1, Hsp90,

Oct, Syndecan-4, Toll-like Receptors


(TLRs), Tubulin, and VDAC.

Please see www.promokine.info/antibodies/subcellular-markers

ROCK-1, SEK1, SLK, Trk and many other


targets.

Antibodies and
ELISAs

Numerous antibodies against AAK1,


Akt/PKB, ALK, AMPK, ASK1, BLK, Brk,
Chk2, DNA-PK, Erk, FAK, HER2/ErbB2,

172

10 Antibodies and ELISAs

https://www.promokine.info/shop

n Anti-Tag Antibodies

Antibodies and
ELISAs

Many antibodies e.g. against FLAG-Tag,


GFP, RFP, GST-Tag, HA-Tag, His-Tag,
Myc-Tag, and MBP.

Please see www.promokine.info/antibodies/anti-tag-antibodies


n Infectious Diseases
Antibodies e.g. against Anthrax, Chlamydia LPS, or Neisseria gonorrhea.

Please see www.promokine.info/antibodies/infectious-diseases


n Obesity
Antibodies e.g. against ELOVL6, GPAT1,
Lipe/HSL and UROP11.

Please see www.promokine.info/antibodies/obesity


n Miscellaneous Antibodies
Antibodies e.g. against Cox, Growth
Hormone (GH), HEP-1, Immunglobu-

lins, Luciferase, Megalin, MOG, MPO,


Plectin, and Syndecan.

Please see www.promokine.info/antibodies/miscellaneous-antibodies

10.2 Antigens and Cell Lysates


The PromoKine peptides are perfectly
suited as blocking reagent in combination with the PromoKine antibodies and
as a control in e.g. immunoblotting/
Western blot, ELISA, or immunohistochemistry assays.
PromoKine also provides a wide range

of ready-to-use cell and tissue lysates


that can be used as controls in e.g. gel
electrophoresis, Western blotting, or immunoprecipitation. Moreover, they are
also useful in enzymatic activity analysis,
protein-protein interaction analysis, and
tissue specific expression identification.

The total tissue and cell line protein lysates are available from human, mouse,
and rat origin as well as from a variety
of developmental stages. Furthermore,
beside lysates from normal tissue also
lysates from tumor tissue and diseased
tissue are available.

For a complete list of our peptides and cell lysates please see www.promokine.info/antigens-and-cell-lysates
or the PromoKine Price List.

10 Antibodies and ELISAs

173

10.3 Labeled Antibodies and Labeling Kits


https://www.promokine.info/shop

n Labeled Secondary Antibodies


(HRP), alkaline phosphatase (AP), or
biotin. All antibodies are purified and
highly active, and they can be used for

Product

Product Description

Donkey Anti-Goat antibody (pAb),


Fluorescein-coupled

diverse applications including ELISAs,


immunofluorescence microscopy, immunohistochemistry, and Western blotting.
Size

Catalog Number*

Donkey Anti-Goat IgG conjugated to Fluorescein


(H&L)

2 mg

PK-AB718-9360

Donkey Anti-Goat antibody (pAb),


Texas Red-coupled

Donkey Anti-Goat IgG conjugated to Texas Red (H&L)

2 mg

PK-AB718-9370

Goat Anti-Chicken antibody (pAb),


Texas Red-coupled

Goat Anti-Chicken IgG conjugated to Texas Red (H&L)

2 mg

PK-AB718-9170

Goat Anti-Mouse antibody (pAb),


AP-coupled

Goat Anti-Mouse IgG conjugated to Alkaline


Phosphatase (H&L, minimum cross-reactivity)

1 mg

PK-AB718-9780

Goat Anti-Mouse antibody (pAb),


Biotin-coupled

Goat Anti-Mouse IgG conjugated to Biotin


(H&L, minimum cross-reactivity)

2 mg

PK-AB718-9740

Goat Anti-Mouse antibody (pAb),


Fluorescein-coupled

Goat Anti-Mouse IgG conjugated to Fluorescein


(H&L, minimum cross-reactivity)

2 mg

PK-AB718-9760

Goat Anti-Mouse antibody (pAb),


HRP-coupled

Goat Anti-Mouse IgG conjugated to Horseradish


Peroxidase (H&L, minimum cross-reactivity)

2 mg

PK-AB718-9720

Goat Anti-Mouse antibody (pAb),


Texas Red-coupled

Goat Anti-Mouse IgG conjugated to Texas Red (H&L)

2 mg

PK-AB718-9770

Goat Anti-Rabbit antibody (pAb),


AP-coupled

Goat Anti-Rabbit IgG conjugated to Alkaline


Phosphatase (H&L, minimum cross-reactivity)

1 mg

PK-AB718-9980

Goat Anti-Rabbit antibody (pAb),


Biotin-coupled

Goat Anti-Rabbit IgG conjugated to Biotin


(H&L, minimum cross-reactivity)

2 mg

PK-AB718-9940

Goat Anti-Rabbit antibody (pAb),


Fluorescein-coupled

Goat Anti-Rabbit IgG conjugated to Fluorescein


(H&L, minimum cross-reactivity)

2 mg

PK-AB718-9960

Goat Anti-Rabbit antibody (pAb),


HRP-coupled

Goat Anti-Rabbit IgG conjugated to Horseradish


Peroxidase (H&L, minimum cross-reactivity)

2 mg

PK-AB718-9920

Goat Anti-Rabbit antibody (pAb),


Texas Red-coupled

Goat Anti-Rabbit IgG conjugated to Texas Red (H&L)

2 mg

PK-AB718-9970

Goat Anti-Rabbit antibody (pAb),


HRP-coupled

Goat Anti-Rabbit IgG conjugated to horseradish


peroxidase

0.5 ml

PK-AB577-6401

Goat Anti-Rat antibody (pAb),


AP-coupled

Goat Anti-Rat IgG conjugated to Alkaline Phosphatase


(H&L, minimum cross-reactivity)

1 mg

PK-AB718-9580

Goat Anti-Rat antibody (pAb),


HRP-coupled

Goat Anti-Rat IgG conjugated to Horseradish


Peroxidase (H&L, minimum cross-reactivity)

2 mg

PK-AB718-9520

PF-XXX-Goat Anti-Human IgG

Conjugated to PromoFluor dye of choice

1 mg

PK-PFXXX-AK-H1

PF-XXX-Goat Anti-Mouse IgG

Conjugated to PromoFluor dye of choice

1 mg

PK-PFXXX-AK-M1

PF-XXX-Goat Anti-Rabbit IgG

Conjugated to PromoFluor dye of choice

1 mg

PK-PFXXX-AK-R1

Rabbit Anti-Chicken antibody


(pAb), AP-coupled

Rabbit Anti-Chicken IgG conjugated to


Alkaline Phosphatase (H&L)

1 mg

PK-AB718-9180

Rabbit Anti-Chicken antibody


(pAb), HRP-coupled

Rabbit Anti-Chicken IgG conjugated to Horseradish


Peroxidase (H&L, minimum cross-reactivity)

1.5 mg

PK-AB718-9120

Rabbit Anti-Goat antibody (pAb),


AP-coupled

Rabbit Anti-Goat IgG conjugated to Alkaline


Phosphatase (H&L, minimum cross-reactivity)

0.5 mg

PK-AB718-9380

Rabbit Anti-Goat antibody (pAb),


HRP-coupled

Rabbit Anti-Goat IgG conjugated to Horseradish


Peroxidase (H&L, minimum cross-reactivity)

1 mg

PK-AB718-9320

Note: Please see our website for an up-to-date list of available labeled antibodies.
*XXX- stands for the respective PromoFluor dye (e.g. PF-350, PF-405, etc.; see page 146).

Antibodies and
ELISAs

PromoKine is offering secondary antibodies which are fluorescently labeled


or conjugated to horseradish peroxidase

174

10 Antibodies and ELISAs

Antibodies and
ELISAs

https://www.promokine.info/shop
Product

Product Description

Size

Catalog Number

Rabbit Anti-Goat antibody (pAb),


HRP-coupled

Rabbit Anti-Goat IgG conjugated to horseradish


peroxidase

0.5 ml

PK-AB577-6403

Rabbit Anti-Mouse antibody (pAb),


HRP-coupled

Rabbit Anti-Mouse IgG conjugated to horseradish


peroxidase

0.5 ml

PK-AB577-6402

n Protein and Antibody Labeling Kits


PromoKine also offers ready-to-use
Protein and Antibody Labeling Kits.
These innovative kits have been

developed for convenient and highly


efficient labeling of specific antibodies or target proteins with our superior

Product

Product Description

PromoFluor-XXX-Labeling-Kit
PromoFluor-XXX-Labeling-Kit

PromoFluor dyes (see page 145).

Size

Catalog Number*

Kit for Labeling of Proteins/Antibodies


with a Selected PromoFluor Dye

5 reactions

PK-PF-LK-XXX-05

Kit for Labeling of Proteins/Antibodies


with a Selected PromoFluor Dye

10 reactions

PK-PF-LK-XXX-10

n ECL Chemiluminescence Detection Kit


High resolution and sensitivity (picogram
to femtogram amounts of target)
Stable hard copy results on film
Long signal duration enables multiple
exposures
Only small amounts of antibody
required for strong signals
Detection possible with short
exposure times (minutes)
Excellent price to performance ratio

PromoKines ECL Chemiluminescence


Detection Kit is a convenient, non-radioactive detection system containing all
the reagents required for highly sensitive
chemiluminescent detection of immobilized proteins (Western blotting) or immobilized nucleic acids (Southern Blotting
or Northern Blotting) conjugated with
HRP (Horse Radish Peroxidase). This
ready-to-use kit allows the detection of

Product

Product Description

ECL Chemiluminescence Detection


Kit

Kit for Chemiluminescent Detection of HRP


or HRP-labeled Biomolecules

membrane immobilized specific antigens


or nucleic acid sequencesdirectly labeled
with HRP or indirectly labeled with HRPtagged antibodies/streptavidin/biotin.

Size

Catalog Number

120 ml
500 ml
1000 ml

PK-MB902-500-120
PK-MB902-500-500
PK-MB902-500-1000

Also available: Prestained RTU Protein Ladders, Fluorescent FluoProtein Gel Stain and GelQuick Protein Gel
Stain/Destaining Kit (see page 123).

*XXX- stands fo the respective PromoFluor dye (PF-405, PF-415, PF-488P, PF-488LSS, PF-514LSS, PF-546, PF-555, PF-633, PF-647, PF-670, PF-750,
PF-770; see page 146) Please see our website for an up-to-date list of available PromoFluor Labeling Kits.

10 Antibodies and ELISAs

175

10.4 ELISA Kits


The ready-to-use ELISA kits are carefully
validated and come with a straightforward and easy-to-use manual as well as
all reagents required to perform a convenient ELISA with reliable and reproducible results. The 96-well assay plate
can either be used at once or a desired
number of assays can be used separately
as the wells in the assay plates can be
detached.

economical in medium- to high-throughput applications. The EDKs are intended


for experienced ELISA users and contain
all required reagents to assay your target
protein/peptide in ten 96-well microtiter
plates (approximately 1,000 assays).
We continuously add new ELISA kits to
our inventory.
For an up-to-date product list please
visit www.promokine.info/ELISAs

In addition, we offer ELISA Development


Kits (EDKs, see page 176) which are very

https://www.promokine.info/shop

n Cell Signaling: Cytokines, Growth Factors, Receptors and Ligands


ELISAs for detection and quantification of human, mouse, rat, monkey, and pig:
Interleukins

e.g. IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-6, IL-7, IL-8, IL-12, IL-13, IL-17, IL-22, and
IL-33

Growth Factors & Colony Stimulating


Factors

e.g. BDNF, CTGF, EGF, FGFs (including FGF-2), G-CSF, GM-CSF, HGF, IGF-1, IGF-BPs,
M-CSF, beta-NGF, PDGF-BB, TGF-beta, VEGF-A, and VEGF-C

Interferons

e.g. IFN-alpha, IFN-gamma

Chemokines

e.g. CRG-2, CTACK, Eotaxin-2, Fractalkine, GRO-alpha, GRO-beta, HCC-4, IP-10,


MCP-1, MCP-2, MCP-5, MDC, MIG, NAP-2, LIX, MIP-1, MIP-3, PARC, RANTES, and
SDF-1 alpha

Tumor Necrosis Factors

e.g. TNF-alpha and TNF-beta

We also supply ELISAs for Angiogenin,


Angiopoietin-1, Angiostatin, BAFF, Bone
Morphogenetic Proteins (BMPs), CRP,
Matrix Metalloproteinases (MMPs),

EPO, flt-3 Ligand, Galectins, ICAMs,


Leptin, LIF, MPO, MSP, Myostatin,
PAI-1, Resistin, sRANKL, SCF, Selectins,
TNF Receptors, TIMP-1 & TIMP-2, TPO,

Please see our website for a complete list of our Cell Signaling ELISAs.
n CD Marker
ELISAs for detection and quantification
of human, mouse and monkey: CD8,
CD23, CD26, CD28, sCD30, CD30L,

CD31, CD40, CD40L, CD44, CD50,


CD54, CD62, CD102, CD106, CD134,
CD137, CD144, CD154, and CD162.

Please see our website for a complete list of our CD ELISAs.


n Hormones
ELISAs for detection and quantification
of human Relaxin, Thymosin alpha-1,
Thymosin beta-4, and Thymulin.

Please see our website for an up-to-date list of our Hormone ELISAs.

VCAM-1, VEGF Receptors and many


more interesting targets.

Antibodies and
ELISAs

PromoKine offers a range of highly sensitive and specific ELISA kits that meet
optimal quality standards. They can be
used for accurate and consistent detection and quantitation of numerous
interesting biomolecules in cell biology
research and are applicable for different
sample material (e.g. cell culture supernatant, serum and plasma) from various
mammalian organisms (human, mouse,
rat, monkey or pig). Some ELISAs can
also be used with cell/tissue lysates,
urine or stool samples.

176

10 Antibodies and ELISAs

https://www.promokine.info/shop

n Apoptosis

Antibodies and
ELISAs

ELISAs for detection and quantification of human and monkey:


Annexin V, APO-1/Fas, APRIL, BAFF,

Bcl-2, Caspase-8 & Caspase-9, Cathepsin


K & Cathepsin L, Cytochrome c, DcR3,
Granzyme A & Granzyme B, LIGHT,

OPG, OX40, TRAIL, VE-Cadherin and


many more interesting targets.

Please see our website for a complete list of our Apoptosis ELISAs.
n Other ELISAs
Here you will find a range of ELISAs for
target molecules which play important
roles in oxidative cell stress and inflammation as well as different metabolism
patways and diseases in the skeletal,
cardiovascular and renal systems. Other
ELISAs included herein can be used for
detecting and quantifying targets which

are essential markers e.g. in gastroenterology, fertility, diabetes, oncology,


clinical immunology and orthomolecular
medicine: ADMA, Adiponectin, Calprotectin, CRP, Myostatin, NT-proCNP,
NT-proANP, Leptin, Matrix Metalloproteinases (MMPs), Osteonectin,
Osteoprotegerin, Oxidized LDL, PAI-1,

sRANKL, RANTES, Thyreoglobulin, Urotensin II, VDBP, Vitamin D, Zonulin and


many more interesting targets.

Please see our website at www.promokine.info/ELISAs for a complete list.


n ELISA Development Kits
Each of PromoKines ELISA Development Kits (EDKs) contains the following components to detect and quantify
the target molecule in at least 10 x 96
ELISA plate wells: Capture antibody,

(biotinylated) detection antibody, avidinperoxidase conjugate and a thoroughly


calibrated standard. A huge variety of
natural and/or recombinant proteins that
play a crucial role in cell signaling can be

assayed in a sandwich ELISA format. The


EDKs come with a straightforward and
easy-to-use instruction manual.

ELISA Development Kits for detection and quantification of human, mouse, and rat:
Interleukins

e.g. IL-1 alpha, IL-2, IL-4, IL-6, IL-7, IL-8, IL-12, IL-13, IL-17, IL-22, IL-33

Growth Factors & Colony Stimulating


Factors

e.g. CTGF, EGF, FGF-2, G-CSF, GM-CSF, beta-NGF, PDGF-BB, PLGF-1, VEGF

Chemokines

e.g. Eotaxin, IP-10, MCP-1, MCP-2, GRO-alpha, GRO-beta, MIP-1, RANTES,


SDF-1 alpha

We also supply EDKs for BD-1, BD-2,


BD-3, BMP-2, CNTF, IFN-gamma,
IGF-BP1, Leptin, NOV, PAI-1, Resistin,

RANKL, SCF, TNF-alpha, TPO and many


more interesting targets.

Please see our website for a complete and up-to-date list of our ELISA Development Kits.

Cytokines and Growth


Factors
Cytokines and chemokines are small proteins secreted by a
variety of cell types. They are important signal messengers
mediating cell communication and activating other target cells
through binding to specific receptors. Cytokines play a central
role in the immune system where they induce inflammatory
and immune responses or function as chemoattractants (chemokines). They also coordinate and regulate other biological
processes such as cell growth and development or tissue repair.

11

178 Content Chapter 11

11

Cytokines and Growth


Factors
11.1 Cytokines, Growth Factors, Hormones

and soluble Receptors . . . . . . . . . . . . . . . . 179
11.2 Animal-free Cytokines and

Growth Factors . . . . . . . . . . . . . . . . . . . . 180
11.3 Cytokines and Growth Factors

for Stem Cell Research . . . . . . . . . . . . . . . 181

11 Cytokines and Growth Factors

179

11.1 Cytokines, Growth Factors, Hormones, and soluble Receptors

Cell-based bioassays
Functional studies
Human, mouse, and rat cell culture
supplements
Immunoassays

Features
High purity and biological activity
Increased stability
Produced in a range of different hosts
Economic bulk sizes are available on
request
PromKine offers an exceptional variety of
recombinant proteins or peptides of human, mouse or rat origin (>800 items!)
which function as signaling molecules in
intercellular communication and play important roles in both health and disease
states. They can initiate, regulate or otherwise affect different effector mecha-

nisms within the responding target cells


by binding to specific cell surface receptors and thus activating intracellular
signal transduction cascades. Other
cytokines mediate chemoattraction between cells (chemokines) and yet other
signaling proteins included in our wide
product range are essential growth factors and hormones which stimulate and
regulate diverse cellular processes such
as cell growth, proliferation, maturation,
and differentiation.
All cytokines, chemokines, growth factors, CD antigens, hormones, and soluble receptors available from PromoKine
meet the highest quality standards. They
are highly purified and biologically active and were produced in E. coli, yeast,
insect cells, plants, or mammalian cell
lines (to provide native posttranslational
modifications if required).
Purity is generally assessed by SDS-PAGE
and HPLC analysis and is in most cases
higher than 95%. Most of the prod-

ucts are delivered lyophilized (and are


very stable in that format), while some
come ready-to-use in a buffer solution.
Reconstitution of the lyophilized proteins
should be carried out in water or common buffer solutions.
Products available at PromoKine include human, mouse and rat Interleukins, Colony Stimulating Factors (CSFs),
Growth Factors, Interferons, Chemokines, Adipokines, Bone Morphogenetic
Proteins (BMPs), Fibroblast Growth Factors (FGFs), Matrix Metalloproteinases
(MMPs), CD antigens, Defensins, Tumor
Necrosis Factors (TNFs), soluble receptors, hormones, extracellular binding
proteins, non-inhibitory Serpins and
other immunoregulatory proteins.
We continuously add new recombinant
proteins to our inventory.
For an up-to-date product list please
visit www.cytokines.info

https://www.promokine.info/shop
Interleukins

e.g. IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12,
IL-13, IL-15, IL-16, IL-17, IL-19, IL-20, IL-21, IL-22, IL-24, IL-31, IL-32, and IL-33

Growth and Differentiation Factors

e.g. BDNF, CTGF, CTGFL, EGF, EG-VEGF, FGFs (including FGF-1, FGF-2 and FGF-4),
GDFs, GDNF, HB-EGF, HDGF, HGF, IGF-1, IGF-2, IGF-BPs, KGF, beta-NGF, PDGFs (including PDGF-AA and PDGF-BB), PLGFs, TGF-betas (including TGF-beta 1), and VEGFs
(including VEGF-A)

Colony Stimulating Factors

e.g. G-CSF, GM-CSF, and M-CSF

Interferons

e.g. IFN-alpha, IFN-beta and IFN-gamma

Chemokines

e.g. BCA-1, BRAK, CTACK, CXCL16, ENA-78, Fractalkine, GCP-2, Eotaxin, Exodus, GROalpha, GRO-beta, GRO-gamma, HCC-1, IP-10, I-TAC, LEC, LIX, Lymphotactin, MCP-1,
MCP-2, MCP-3, MCP-4, MCP-5, MDC, MEC, MIG, MIP-1, MIP-3, MIP-4, NAP-2, PF-4,
RANTES, SDF-1 alpha, SDF-1 beta, TAFA, TARC, and TECK

Tumor Necrosis Factors

e.g. TNF-alpha and TNF-beta

Bone Morphogenetic Proteins

e.g. BMP-2, BMP-3, BMP-4, BMP-5, BMP-6, and BMP-7

Matrix Metalloproteinases

e.g. MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, and MMP-13

Hormones

e.g. Adiponectin, CNTF, EPO-alpha, FSH, GH, Ghrelin, Insulin, Leptin, Prolactin, Relaxin,
Resistin, and TPO

CD Antigens

e.g. CD1A, CD2, CD4, CD9, CD11b, CD14, CD22, CD23, CD25, CD29, CD31, CD34,
CD43, CD45, CD54, CD56, CD62, CD68, CD72, CD95, CD99, CD100, CD105, CD106,
CD120, CD124, CD137, CD141, CD146, CD147, CD152, CD154/CD40L, CD269, and
CD283

We also supply other important signaling proteins/peptides such as:


Activin A, APRIL, Angiogenin, Angiopoietin-1, Angiostatin, Apolipoproteins,
Artemin, BAFF, Betacellulin, Cardiotrophin-1, CRP, DKK-1, Endostatin, FGF

Receptors, flt-3 Ligand, Follistatin, Galectins, Granzyme B, Heregulin, ICAMs,


LIF, Maspin, Midkine, Myostatin, Nanog,
Neuregulin, Neuroglobin, Neuroserpin,
Neurotrophins, NOGGIN, Oncostatin M,
OPG, Osteonectin, Osteopontin, PAI-1

Please see www.cytokines.info for a complete and up-to-date list.

& PAI-2, RANKL, RELM-beta, SCF, Selectins, TNF Receptors, TIE-1 & TIE-2,
TIMP-1 & TIMP-2, TRAIL, VCAM-1,
VEGF Receptors, Vaspin, Vitronectin,
Visfatin, Wnt-3a and many more interesting targets.

Cytokines and
Growth Factors

Applications

180

11 Cytokines and Growth Factors

11.2 Animal-free Cytokines and Growth Factors


Applications
Cell-based bioassays
Functional studies
Human, mouse, and rat cell culture
supplements
Immunoassays

Cytokines and
Growth Factors

Features
High purity and biological activity
Increased stability
Free of any impurities derived from
animals or bacteria
Economic bulk sizes are available on
request
PromoKines animal-free cytokines and
growth factors have been produced by
molecular farming using tobacco plants

or barley grain as the production hosts which have the G.R.A.S. (Generally Recognized As Safe) status from the FDA.
These cytokines are free of animal
components, serum, endotoxins, and
antibiotics, as well as human or animal
infectious agents or other endogenous
mammalian contaminants (e.g. traces
of unwanted growth factors and cytokines). As these proteins have been
expressed in plants, they show very low
proteolytic, pyrogenic, and inflammatory activity compared to other commercially available proteins such as those
produced in bacteria, yeast, insect, or
mammalian cells. In addition, they retain
proper eukaryotic folding and glycosylation resulting in an increased stability
and biological activity. Degradation due
to protease carryover as well as un-

wanted cell responses (e.g. proliferation


or inflammatory cascades) or cell growth
inhibition caused by contaminating serum components (serum carryover),
endotoxins or bacterial, viral, and prion
infectious agents are also eliminated or
at least extremely reduced.
PromoKines range of plant-derived
proteins were produced under strict
animal-free conditions. While avoiding
any potential variables and interfering effects associated with the use of
animal-derived manufacturing components they still maintain high biological activity and purity compared to the
corresponding proteins produced using
standard techniques. Thus, they are ideally suited for some very sensitive applications, e.g. in stem cell research.

https://www.promokine.info/shop
Our range of animal-free cytokines and growth factors is continuously growing and includes:
Interleukins

e.g. IL-2, IL-3, IL-4, IL-6, IL-12

Growth Factors & Colony Stimulating


Factors

e.g. FGF-2, KGF, TGF-beta 2 and TGF-beta 3, and VEGF-165

Colony Stimulating Factors

e.g. GM-CSF, and M-CSF

Interferons

e.g. TNF-alpha

Bone Morphogenetic Proteins

e.g. BMP-2 and BMP-7

We also supply other important signaling proteins/peptides such as: Activin A,


Activin B, BAFF, flt-3 Ligand, Follistatin,

Growth Hormone, LIF, Myostatin,


sRANKL, SCF and many more interesting
targets that will come soon.

Please see www.promokine.info/AF for a complete and up-to-date list.

OD (490 nm)
1.6
1.5

Control fraction
PromoKines animal-free VEGF-121

1.4
1.3
1.2
0.1

Fig. 1: Cell proliferation assay


using PromoKines animal-free
VEGF-121 and HUVEC cells (see
page 13)

1.0
0.9
0.8
0.7
0.0

10-

10-

10-

1.0

10

ng/ml VEGF-121

10

10

11 Cytokines and Growth Factors

181

Stem cells play a central role in the development of multicellular organisms


and diverse regeneration processes
throughout their lifes, e.g. replacing
diseased and damaged tissues. They are
unspecialized precursor cells characterized by their abilities of self-renewal
and pluripotency, that means they can
undergo numerous rounds of cell division maintaining the undifferentiated
state and are able to develop into different types of cells such as skin, muscle or
blood cells.

Mammalian stem cells are generally


comprised of two types: Embryonic stem
cells (ESCs) are derived from the epiblast
tissue of the inner cell mass of an early
stage embryo (blastocyst) and are considered pluripotent and capable to differentiate into any cell type of the adult
body. Adult stem cells (also known as
somatic stem cells) are undifferentiated
cells found in the tissues of children and
adults. Their main function is to replace
dead or damaged specialized cells and
to maintain the normal turnover of re-

generative organs. Most adult stem cells


are lineage-restricted (multipotent) and
characterized by a limited self-renewal
and differentiation capacity usually
restricted to cell types of the tissue in
which they reside. In contrast, induced
pluripotent stem cells (iPSCs) are not
adult stem cells but specialized somatic
adult cells (e.g. epithelial cells) that have
been genetically reprogrammed to pluripotent cells which are in many respects
similar to natural pluripotent stem cells
such as embryonic stem cells.

https://www.promokine.info/shop
PromoKine provides a wide range of cytokines and growth factors (>300) which are important in stem cell research:
Mesenchymal Stem Cell Growth and Differentiation Factors

e.g. EG-VEGF, FGF-4, flt-3 Ligand, GDFs, GM-CSF, HGF, IFN-gamma, IL-6, SDF-1 alpha,
TGF-beta 2, and TGF-beta 3

Cytokines & Growth Factors for General


Propagation and Differentiation of Various
Adult Stem Cell Types

e.g. BAFF, BDNF, beta-NGF, BMP-2, BMP-4, BMP-7, EGF, FGF-2, flt-3 Ligand, G-CSF,
GM-CSF, IGF-1, IL-3, IL-6, IL-11, M-CSF, PDGF-AA, PDGF-BB, SCF, TARC, TGF-beta 1,
TPO, and VEGFs (e.g. VEGF-A and VEGF-C)

Cytokines for Propagation of Undifferentiated Pluripotent Human Embryonic Stem


Cells

e.g. Ang-1, LIF, Nanog, Wnt-1, and Wnt-3a

Hematopoietic Stem Cell Research

e.g. Ang-1, EPO-alpha, flt-3 Ligand, G-CSF, GM-CSF, Granzyme B, IFN-gamma, IGF-1,
IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, PDGF-AA, PDGF-BB, SCF, SDF-1 alpha,
TGF-alpha, TGF-betas (1-3), TNF-alpha, TPO, and VEGFs (e.g. VEGF-121 and VEGF165)

General Embryonic Stem Cell Research

e.g. Activin A, Activin B, Ang-1, BDNF, BMP-4, BMP-7, EGF, FGFs (e.g. FGF-2 and FGF-4),
GDF-3, Heregulin-beta, IGF-1, KGF, Noggin, Oncostatin M, TGF-alpha, TGF-beta 2, TGFbeta 3, and Vitronectin

Neural Stem Cell Research

e.g. FGF-2, EGF, Galectin-1, and Wnt-3a

Please see www.promokine.info/stemcells for a complete and up-to-date list.


Note: Find more products for stem cell research (Antibodies, Stem Cell Fate Regulators, etc.) on our website.

Cytokines and
Growth Factors

11.3 Cytokines and Growth Factors for Stem Cell Research

Microbial Detection
and Elimination
Cell culture contamination is a common problem that impacts
many biochemical and immunological properties of cells and it
leads to unsatisfactory and unreliable results. Therefore, regular
testing of cell cultures for mycoplasmas/bacteria contamination
is essential.
PromoKine provides highly sensitive and user-friendly test kits
for this purpose, as well as products for the effective elimination
and prevention of mycoplasma and eubacteria contamination.

12

184 Content Chapter 12

12

Microbial Detection
and Elimination
12.1 Mycoplasma Test Kits . . . . . . . . . . . . . . . 185
12.2 Mycoplasma Elimination Solutions . . . . . . 186
12.2.1 Mycoplasma-Ex . . . . . . . . . . . . . . . . . . . . . 187
12.2.2 BIOMYC Antibiotic Solutions . . . . . . . . . . . 187

12.3 Bacteria Test Kit . . . . . . . . . . . . . . . . . . . . 188


12.4 Disinfectants . . . . . . . . . . . . . . . . . . . . . . . 189
12.4.1 Mycoplasma-Ex Spray . . . . . . . . . . . . . . . . 189
12.4.2 Pharmacidal Spray . . . . . . . . . . . . . . . . . . . 189
12.4.3 Aquaguard . . . . . . . . . . . . . . . . . . . . . . . . . 190
12.4.4 Mycoplasma-EX Wipes . . . . . . . . . . . . . . . . 190

12 Microbial Detection and Elimination

185

12.1 Mycoplasma Test Kits

Mycoplasma

detection in cell culture,


virus stocks, and medium
Conventional and real-time PCR

Features
Highly

sensitive (detects 10 - 15
mycoplasmas per sample volume)
Fast, reproducible, and reliable results
Applicable for all cell/tissue culture relevant mycoplasma species
Positive and internal controls included
Convenient protocol and easy sample
preparation
Mycoplasmas are parasitic bacteria that
frequently contaminate tissue cultures
and consequently also virus stocks, vaccines, and other biological materials produced in cells. The contamination can
originate from serum, laboratory staff,
other contaminated cultures, or the donors from which the cells have been harvested, and it often remains unnoticed.
Although mycoplasmas do not cause
visible cell damage, they seriously affect
cell growth and metabolism, transfection efficiency, protein and monoclonal
antibody synthesis, cytokine secretion,
immunological properties, signal transduction, virus proliferation and can even
cause DNA and RNA damage.
Since mycoplasmas are very small and
lack cell walls, detection using a conventional light microscope is nearly impossible. In addition, cloudiness in the

medium, typical for bacterial or yeast


contaminations, does not occur even at
high mycoplasma concentrations. PCR
on the other hand is a highly sensitive,
specific, and rapid method for the detection of mycoplasma contamination
in cell culture supernatant, cell/virus
stocks, and medium. All cell/tissue culture relevant mycoplasma species can
be detected in one assay including M.
fermentans, M. hyorhinis, M. arginini,
M. orale, M. salivarium, M. hominis,
M. pulmonis, and M. pirum. In addition, Acholeplasma laidlawii, M. synoviae, and Ureaplasma species can be
detected.

PCR Mycoplasma Test Kit I/C


The PCR Mycoplasma Test Kit I/C utilizes conventional PCR technology for
convenient and specific detection of all
known cell culture-contaminating mycoplasma species. The primer set is specific for the highly conserved 16S-rRNA
coding region in the mycoplasma
genome. The test can be performed in 3
hours and includes internal control DNA
(for verifying a successful PCR run) as
well as positive control DNA. All components required for the PCR (primers,
nucleotides, internal control DNA) come
optimally premixed and lyophilized in
PCR tubes (except the Taq polymerase).
For setting up the PCR, simply cut off
the number of tubes required, rehydrate
with rehydration buffer (containing the
Hot-Start Taq polymerase), and add the

sample to be analyzed. Samples can be


easily prepared by boiling the cell culture
supernatant or other sample material.

PCR Mycoplasma Test Kit I/RT


The PCR Mycoplasma Test Kit I/RT utilizes Real-Time PCR technology for convenient and reproducible detection of
contaminating mycoplasmas. It shows
excellent sensitivity and specificity and
enables reliable, quantitative PCR results quickly (making the kit particularly
useful for routine testing). The primer
and probe set is specific for the highly
conserved 16S-rRNA coding region in
the mycoplasma genome. It contains
FRET probes emitting fluorescent light
at 520 nm and is compatible with most
common Real-Time PCR cyclers (e.g.
Roche Light Cycler 1 and 2, ABI Prism,
Cepheid Smart Cycler, and Eppendorf
Mastercycler ep realplex).
The test includes internal control DNA
(for verifying a successful PCR run) as
well as positive control DNA. All components required for Real-Time PCR
(primers, probes, nucleotides, and internal control DNA) come lyophilized
in PCR tubes, except the Hot-Start Taq
polymerase which is dissolved in reaction buffer. Two kit variants (kit A for
e.g. Light Cycler, kit B for e.g. ABI Prism)
are provided which have been optimized
for different Real-Time PCR instruments.
For recommended kit variant, please see
www.promokine.info where you can
download the manual.

PCR
100 l cell culture
supernatant

2 l supernatant

Analyze 5 l of
the PCR sample

Boil for 10 minutes


and centrifuge briefly

Fig. 1: Mycoplasma detection with the PromoKine PCR Mycoplasma Test Kit I/C is easy and convenient: Boil 100 l cell culture
supernatant or other sample material for 10 minutes; centrifuge briefly and add 2 l supernatant to the reaction cup with the
rehydrated PCR components and Taq polymerase; start PCR run; analyze PCR products by agarose gel electrophoresis. When
using the PCR Mycoplasma Test Kit II, the sample is directly added to the ready-to-use, complete reaction mix to set up the PCR.

Microbial Detection
and Elimination

Applications

186

12 Microbial Detection and Elimination

PCR Mycoplasma Test Kit II

The kit includes all components required


for PCR (nucleotides, primers, a highperformance Taq polymerase, magnesium and an agarose gel loading dye)
premixed at optimized concentrations
in a ready-to-use master mix. The test
takes approximately five hours to obtain clear results and reliably detects all

Microbial Detection
and Elimination

The PCR Mycoplasma Test Kit II utilizes conventional PCR technology for
convenient and reliable detection of
mycoplasmas. The primer set is specific
for the highly conserved 16S-rRNA coding region in the mycoplasma genome.

mycoplasma species commonly found in


contaminated cell cultures.
The kit also contains positive control
DNA for easy result verification but, different to the PCR Mycoplasma Test Kit
I/C, contains no internal control DNA.

Fig. 2: Detection of mycoplasmas with


PCR Mycoplasma Test Kit I/C.
M: 100 bp DNA ladder
1: Positive control
2-6: 10; 100; 1,000; 10,000; 100,000
mycoplasmas/sample volume
Internal control DNA: 479 bp band
Positive sample/control: 270 bp band

479 bp
270 bp

https://www.promokine.info/shop
Product

Size

Catalog Number

PCR Mycoplasma Test Kit I/C

24 tests
48 tests
96 tests

PK-CA91-1024
PK-CA91-1048
PK-CA91-1096

PCR Mycoplasma Test Kit I/RT; Variant A

25 tests

PK-CA91-3025A

PCR Mycoplasma Test Kit I/RT; Variant B

25 tests

PK-CA91-3025B

PCR Mycoplasma Test Kit II

20 tests

PK-CA20-700-20

Mycoplasma fermentans DNA Standard


(for qPCR)

100 l

PK-CA91-0117

Mycoplasma orale DNA Standard (for


qPCR)

100 l

PK-CA91-0112

RTU DNA Ladder I (100 bp)

0.5 ml

PK-MB713-001N

RTU DNA Ladder II (1000 bp)

0.5 ml

PK-MB713-002N

Please see page 125 for the ready-to-use DNA Ladders.

12.2 Mycoplasma Elimination Solutions


Mycoplasma contamination seriously affects cell growth and cell physiology leading to biased and unreliable experimental
results. When mycoplasma contamination is detected, the contaminated cells
are ideally discarded and all equipment,
e.g. work benches, CO2 incubators, etc.,

are disinfected (see page 189 for disinfectant sprays). However, if the affected cells are unique or the result of
extensive work, thorough elimination of
the contaminating mycoplasmas is necessary. Standard antibiotics commonly
used in cell culture (e.g. Pen/Strep) do

not affect mycoplasmas. Therefore,


PromoKine offers two highly effective
methods to get rid of mycoplasma contamination: Mycoplasma-EX, a combination of non-antibiotic and antibiotic
reagents, and BIOMYC 1-3, three highly
effective antibiotics.

12 Microbial Detection and Elimination

187

12.2.1 Mycoplasma-EX

Mycoplasma

elimination in cell/tissue
culture
Mycoplasma elimination in virus stocks

Features
Highly

effective against mycoplasma


species commonly found in cell
cultures
Very short treatment time
Very low cytotoxicity
Ready to use
Easy handling
Mycoplasma-EX combines a non-antibiotic and an optimized antibiotic treat-

ment for the effective elimination of mycoplasmas and related microorganisms


from cell/tissue culture and virus stocks.
It eliminates mycoplasmas by directly
killing them and not just by inhibiting
their growth, with minimal toxicity for
the cells in culture.
Mycoplasma-EX is effective after a single
treatment and destroys mycoplasmas
rapidly and permanently. It is applicable
for most cell lines (e.g. Vero, BHK21,
GBK, ML, Hep2, 293, CRFK, H9, Molt4,
MT-4, Jurkat), primary cells as well as
virus stocks (e.g. SHV-1, BHV-1, HSV-1,
VSV, SFV, FCV, MEV) and is highly effective against M. orale, M. hyorhinis,
M. hominis, Acholeplasma laidlawii,
M. arginini, M. fermentans, and other

mycoplasma species encountered as


contaminants in cell cultures.

Fig. 3: Fluorescence microscope image of


cells contaminated with mycoplasmas.
Picture kindly provided by M. Ebert,
Forschungszentrum fr Medizintechnik
und Biotechnologie, Erfurt, Germany.

12.2.2 BIOMYC Antibiotic Solutions


Applications
Mycoplasma

elimination in cell/

tissue culture

In addition, they frequently just reduce


the concentration of mycoplasmas rather
than completely eliminating them. As
soon as treatment is discontinued, contamination will recur.

Features
BIOMYC-1 and 2
Highly

effective against mycoplasma


species commonly found in cell
cultures
Low cytotoxicity
Ready to use
Easy handling
Antibiotics that impact bacterial cell walls
(e.g. penicillin) are ineffective against
mycoplasmas which lack a cell wall.
Other antibiotics such as tylosin, neomycin, tetracycline, or gentamicin are
only effective against a few mycoplasma
species commonly found in cell cultures.

BIOMYC-1 and 2 are two antibiotics


which eliminate mycoplasmas very efficiently when used in combination. After
a four-day treatment with BIOMYC-1,
a three-day treatment with BIOMYC-2
follows. This is repeated 2 to 3 times.
Following this protocol, mycoplasmas
will not develop resistance to these antibiotics - a common occurrence with
other antibiotic treatment methods.
BIOMYC-1 is based on the antibiotic tiamutin which is produced by the fungus
Pleurotus mutilus. BIOMYC-2 is based

on the antibiotic minocycline, an improved derivative of tetracycline.

BIOMYC-3
BIOMYC-3 is based on the antibiotic
ciprofloxacin which is a member of the
fluoroquinolone group and inhibits
synthesis of DNA gyrase, an enzyme
necessary for bacterial DNA supercoiling. Many mycoplasma species are
sensitive to BIOMYC-3, including M.
orale, M. hyorhinis, M. fermentans, and
M. arginini, and also Acheloplasma laidlawii. These species are responsible for
most mycoplasma contaminations in cell
cultures. No cytotoxic effects on the cells
in culture have been found at the concentrations recommended for use, and
the treatment can be performed within
12 days.

https://www.promokine.info/shop
Product

Size

Catalog Number

3 treatments

PK-CC91-4003

BIOMYC-1

10 ml
20 ml
100 ml

PK-CC03-036-1D
PK-CC03-036-1C
PK-CC03-036-1B

BIOMYC-2

10 ml
20 ml
100 ml

PK-CC03-037-1D
PK-CC03-037-1C
PK-CC03-037-1B

BIOMYC-3

10 ml
20 ml
100 ml

PK-CC03-038-1D
PK-CC03-038-1C
PK-CC03-038-1B

Mycoplasma-EX

Microbial Detection
and Elimination

Applications

188

12 Microbial Detection and Elimination

12.3 Bacteria Test Kit


Applications
Bacteria detection in cell culture, virus
stocks, and medium

Features
Highly

Microbial Detection
and Elimination

sensitive (detects 12 bacteria


genomes per sample volume)
Reproducible and reliable results
Applicable for 45 bacteria species
Positive and internal controls included
Convenient protocol and easy sample
preparation

cobacterium, Peptostreptococcus, Pseudomonas, Porphyromonas, Prevotella,


Salmonella, Serratia, Staphylococcus,
Streptococcus, etc.). The kit provides a
highly sensitive, easy, and rapid method
to detect bacterial infection in various biological materials, including cell cultures.
Detection requires as little as 12 bacteria
genomes (~52 fg) per sample volume.
The protocol can be completed within
3.5 hours with a hands-on time of less
than 40 minutes.

decoloration of the medium. In addition,


it has been demonstrated that standard
antibiotics are mostly ineffective against
resistant bacterial infection but have
a strong impact on the metabolism,
growth, and differentiation of the cells
in culture.

Bacteria are frequently found as contaminants in cell cultures. However, cell


cultures sometimes lack visual signs of
the bacterial contamination, such as fast

Frequent screening of cell cultures for


bacterial contamination is essential
to ensure reliable results. Therefore,
PromoKine offers a PCR-based Bacteria
Test Kit that has been developed for
accurate and reliable in vitro diagnosis of
45 bacteria species which are the common contaminants in cell cultures (e.g.
Actinomyces, Bacillus, Enterococcus,
Escherichia coli, Fusobacterium, Klebsiella, Lactobacillus, Micrococcus, My-

The kit contains all the necessary components for setting up a PCR - including a
positive control DNA as well as an internal control DNA to confirm a successfully
performed PCR run. The kit does not
amplify eukaryotic DNA.

PCR
200 - 500 l cell
culture supernatant

Analyze 5 l of
the PCR sample

5 l DNA sample
Extract Bacterial
DNA

Fig. 4: Bacteria detection with the PromoKine PCR Bacteria Test Kit is easy and convenient: Purify bacterial DNA from 200 500 l
of cell culture supernatant using an appropriate DNA extraction kit; add 5 l sample DNA to the reaction cup with the premixed
PCR components and Taq polymerase; start PCR run; analyze PCR products by agarose gel electrophoresis.

https://www.promokine.info/shop
Product

Size

Catalog Number

PCR Bacteria Test Kit

24 tests

PK-CA91-2024

PCR Bacteria Test Kit

48 tests

PK-CA91-2048

PromoKine also offers a Bacteria Live/Dead Staining Kit (see page 105) as well as fluorescent dyes for staining of bacteria (see
page 107).

12 Microbial Detection and Elimination

189

12.4 Disinfectants
Disinfection of all tissue culture equipment and work surfaces in the lab has
to be performed regularly to prevent
microbial contamination. PromoKine of-

fers different disinfectants ideal for laboratory surfaces and apparatuses, CO2
incubators, and water baths.

For more information please see


www.promokine.info/knowledge-base

Mycoplasma-EX Spray allows safe disinfection and cleaning of all laboratory surfaces and apparatuses, including work
benches, incubators, cell storage boxes,
and liquid nitrogen containers. It utilizes
the powerful Mycoplasma-EX reagent in
combination with an alcohol-based solution. Besides its strong mycoplasmacidal
activity, it is very effective e.g. against
Staphylococcus aureus, Streptococcus
faecalis, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Candida

albicans, Mycobacteria, HBV/HIV, Papova, Adeno, Polio and Vaccinia viruses.


The effectiveness of Mycoplasma-EX
Spray is further enhanced by its excellent
detergent properties, achieving clean and
contamination-free surfaces in one step.

Applications
Disinfection
Disinfection

of all laboratory surfaces


of laboratory apparatuses

Features
Kills

mycoplasmas within seconds


against most microorganisms
Non-corrosive and non-cancerogenic
Does not leave any residue
Supplied in a refillable spray bottle
Effective

https://www.promokine.info/shop
Product
Mycoplasma-EX Spray
Mycoplasma Disinfection Kit I
(Consists of Mycoplasma-Ex Spray 500 ml,
Aquaguard-1, and Aquaguard-2)

Size

Catalog Number

500 ml
5L

PK-CC91-5050
PK-CC91-5050-5L

1 Kit

PK-CC91-5060

12.4.2 Pharmacidal Spray


Applications

Non-corrosive

and non-cancerogenic
not leave any residue
Supplied in a refillable spray bottle
Does

Disinfection

of incubators

Features
Free

of mercury, formaldehyde,
phenol, and alcohol
Very effective against bacteria, fungi,
spores, and viruses

Pharmacidal Spray is a mercury-, formaldehyde-, phenol-, and alcohol-free


disinfectant specifically designed for the
disinfection of incubators. Spraying the
incubators thoroughly every two weeks
is recommended.

https://www.promokine.info/shop
Product
Pharmacidal Spray
Mycoplasma Disinfection Kit II
(Consists of Pharmacidal Spray 1 L,
Aquaguard-1, and Aquaguard-2)

Size

Catalog Number

1000 ml
5L

PK-CC-110100
PK-CC-110100-5L

1 Kit

PK-CC91-5070

Microbial Detection
and Elimination

12.4.1 Mycoplasma-EX Spray

190

12 Microbial Detection and Elimination

12.4.3 Aquaguard
Applications
Disinfection

of water trays in CO2


incubators
Disinfection of water baths

Microbial Detection
and Elimination

Features
Very

effective against bacteria, fungi,


spores, and viruses
Non-corrosive
Non-toxic and non-volatile

The water in an incubator or water bath


is a major source of cell culture contamination, particularly with respect
to mycoplasma contaminations, since
mycoplasmas can survive in pure water.
Aquaguard, a non-volatile disinfectant,
is extremely effective at eliminating the
microorganisms in water. In addition, it
does not cause damage to the stainless
steel of the incubator or any plastic interior and it does not cause any skin irritation when used as recommended.

Aquaguard-1 is intended for disinfecting water in incubators, whereas Aquaguard-2 is intended for water bath disinfection.
Both solutions keep the water reliably
free of undesired microorganisms.

https://www.promokine.info/shop
Product

Size

Catalog Number

100 ml
10 x 100 ml

PK-CC01-867-1B
PK-CC01-867-10B

50 ml
10 x 50 ml

PK-CC01-916-1E
PK-CC01-916-10E

Mycoplasma Disinfection Kit I


(Consists of Mycoplasma-Ex Spray 500 ml,
Aquaguard-1, and Aquaguard-2)

1 Kit

PK-CC91-5060

Mycoplasma Disinfection Kit II


(Consists of Pharmacidal Spray 1 L,
Aquaguard-1, and Aquaguard-2)

1 Kit

PK-CC91-5070

Aquaguard-1 (incubator)
Aquaguard-2 (water bath)

12.4.4 Mycoplasma-EX Wipes


Mycoplasma-EX Wipes are an effortless
way to clean and disinfect a variety of
laboratory surfaces such as work benches, pipettors, cell storage boxes, the
incubator interior, etc. There is no need
for multiple products when you have the
convenience of a simple-to-use wipe.
Just wipe, toss and youre done!
Similar to our Mycoplasma-EX Spray,
Mycoplasma-Ex Wipes work efficiently

against mycoplasmas, fungi, bacteria,


viruses and prevent any microbial contamination. Mycoplasma-Ex Wipes contain an alcohol-based, non-corrosive and
non-carcinogenic solution with a reagent
that kills mycoplasmas effectively and
fast.
Mycoplasma-EX Wipes are excellent for
maintaining a clean, contamination-free
work area and also allow fast and easy

cleaning and disinfection of alcoholresistant surfaces. They can be used


where it is not possible to spray and
where rapid drying is important.
Mycoplasma-EX Wipes are supplied as
single-use wipes in a convenient product
safety container. Economic refill sachets
are also available.

https://www.promokine.info/shop
Product
Mycoplasma-EX Wipes

Size

Catalog Number

120 pieces
5 x 120 pieces

PK-CC91-5055
PK-CC91-5055-5

Alphabetical Index

191

Description

Page

1-9

Description

Page

Annexin V-Biotin Detection Kit

133

Annexin V-Biotin Reagent

133

4-Bromo A-23187 free acid

118

Annexin V-Cy3 Detection Kit

132

5(6)-CFDA

117

Annexin V-Cy3 Detection Kit Plus

132

5(6)-CFDA, SE (CFSE)

117

Annexin V-Cy3 Reagent

133

5-(and-6)-Carboxy-2',7'-dichlorofluorescein

117

Annexin V-Cy5 Detection Kit

132

5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate

117

Annexin V-Cy5 Reagent

133

5-(and-6)-Carboxyfluorescein

117

Annexin V-EGFP Detection Kit

132

5,5'-dibromo-Bapta (tetrapotassium salt)

116

Annexin V-EGFP Reagent

133

5,5'-difluoro-Bapta (tetrapotassium salt)

116

Annexin V-FITC Detection Kit

132

5,5'-difluoro-Bapta AM

116

Annexin V-FITC Detection Kit Plus

132

5,5'-dimethyl BAPTA, AM ester

116

Annexin V-FITC Reagent

133

5,5'-dimethyl BAPTA, tetrapotassium salt

116

Annexin V-PE Detection Kit

133

5-Carboxy-2',7'-dichlorofluorescein

117

Annexin V-PE Detection Kit Plus

133

5-Carboxy-2',7'-dichlorosulfonefluorescein

117

Annexin V-PE Reagent

133

5-CFDA

117

Annexin V-PE-Cy5 Detection Kit

133

5-FAM

117

Annexin V-PE-Cy5 Reagent

133

6-CFDA

117

Antibiotics and Antimycotics

97

6-FAM

118

Antibodies

169

7-AAD (7-aminoactinomycin D)

109

Antigens

172

Aortic Adventitial Fibroblasts

28

Aortic Endothelial Cells

15

Aortic Smooth Muscle Cells

37

A
A-23187 free acid

118

Apoptosis

128

Accutase-Solution

95

Apoptosis antibodies

169

Acridine Orange (AO) Solution

109

Apoptosis ELISAs

176

Actinomycin D

141

Apoptosis Inducer Set

141

Actinomycin D (10 mM)

141

Apoptotic Cell Isolation Kit

141

Adipocyte Media

77

Apoptotic DNA Ladder Detection Kit I

129

Adipogenic Differentiation Medium

80

Apoptotic DNA Ladder Detection Kit I plus

129

ADP Fluorometric & Colorimetric Quantitation Kit

121

Apoptotic DNA Ladder Detection Kit II

129

ADVASEP-7 (sulfobutyl b-cyclodextrin)

112

Apoptotic/Necrotic Cells Detection Kit

140

Airway Epithelial Cell Media

65

Apoptotic/Necrotic/Healthy Cells Detection Kit

140

AK Cytotoxicity Kit

106

Aquaguard-1 (incubator)

190

Akt Activated Cell Lysate

139

Aquaguard-2 (water bath)

190

Akt Activity Detection Kit

139

ATP Fluorometric & Colorimetric Quantitation Kit

121

Akt Inhibitor

139

Akt Negative Control Cell Lysate

139

Allophycocyanin

152

Alpha-Bungarotoxin (from Bungarus multicinctus)

112

Bacteria Live/Dead Staining Kit

105

AM1-43

112

Bapta (tetracesium salt)

116

AM1-44

112

Bapta (tetrapotassium salt)

116

AM2-10

112

Bapta (tetrasodium salt)

116

AM3-25

111, 112

Bapta AM

116

AM4-64

112

Bapta-FF (free acid)

116

AM4-66

112

Bapta-FF AM

116

Amino Acid Solutions

99

BCECF

110, 118

Amphotericin B Solution

97

BCECF AM

110, 118

Anisomycin

141

BCECF AM Solution

110, 118

Annexin V Binding Buffer

133

beta-Galactosidase Reporter Assay Kit

108

Annexin V Unlabeled Reagent

133

beta-Galactosidase Staining Kit

108

Appendix

Alphabetical Index

Appendix

192

Alphabetical Index

Description

Page

Description

Page

Beta-Secretase Fluorometric Assay Kit

119

Cardiac Fibroblasts

28

Beta-Secretase Inhibitor I

119

Cardiac Microvascular Endothelial Cells

20

Beta-Secretase Inhibitor II

119

Cardiac Myocytes

11

Betulinic acid

141

Caspase Inhibitors

136

Biocytin (e-biotinoylL-lysine)

113

Caspase Screening Kit

135

Biocytin-Hydrazide

113

Caspase Substrates

136

Bioluminescent Cell Viability Kit I (ATP)

104

Caspase-1 Colorimetric Assay Kit

137

Bioluminescent Cell Viability Kit II (ADP/ATP)

104

Caspase-1 Drug Screening Kit

135

BIOMYC-1

187

Caspase-1 Fluorometric Assay Kit

137

BIOMYC-2

187

Caspase-10 Colorimetric Assay Kit

137

BIOMYC-3

187

Caspase-10 Drug Screening Kit

135

Biotin PCR Labeling Kit

149

Caspase-10 Fluorometric Assay Kit

137

Biotin-4-Fluorescein

113

Caspase-12 Fluorometric Assay Kit

137

Biotin-AM3-25

112

Caspase-2 Colorimetric Assay Kit

137

Biotin-dATP (PCR)

151

Caspase-2 Drug Screening Kit

135

Biotin-dCTP (PCR)

151

Caspase-2 Fluorometric Assay Kit

137

Biotin-DHPE

111

Caspase-3 Colorimetric Assay Kit

137

Biotin-dUTP (PCR)

151

Caspase-3 Drug Screening Kit

135

Biotin-Ethylenediamine, hydrobromide

113

Caspase-3 Fluorometric Assay Kit

137

Biotin-Ethylenediamine, hydrochloride

113

Caspase-3 Fluorometric Assay Kit (HTS)

137

Biotin-Rhodamine 110

113

Caspase-3 Immunoassay Kit

137

Biotin-X-DHPE

111

Caspase-4 Colorimetric Assay Kit

137

Biotin-XX-a-Bungarotoxin

112

Caspase-4 Drug Screening Kit

135

Bis-Fura-2, hexapotassium salt

116

Caspase-4 Fluorometric Assay Kit

137

Bladder Microvascular Endothelial Cells

20

Caspase-5 Colorimetric Assay Kit

137

Bladder Smooth Muscle Cells

40

Caspase-5 Drug Screening Kit

135

Blood Cell Separation Medium

84

Caspase-5 Fluorometric Assay Kit

137

Blood Cells

48

Caspase-6 Colorimetric Assay Kit

137

Blue-fluorescent Cytoplasmic Membrane Staining Kit

111

Caspase-6 Drug Screening Kit

135

Bovine Pituitary Extract

99

Caspase-6 Fluorometric Assay Kit

137

B-Phycoerythrin

152

Caspase-7 Drug Screening Kit

135

Brain Microvascular Endothelial Cells

22

Caspase-7 Immunoassay Kit

137

Brefeldin A

141

Caspase-8 Colorimetric Assay Kit

137

Bronchial Epithelial Cells

24

Caspase-8 Drug Screening Kit

135

Bronchial Smooth Muscle Cells

40

Caspase-8 Fluorometric & Colorimetric Assay Kit

137

Buffers and Salt Solutions

98

Caspase-8 Fluorometric Assay Kit

137

Caspase-8 Fluorometric Assay Kit (HTS)

137

Caspase-9 Colorimetric Assay Kit

137

Caspase-9 Drug Screening Kit

135

C
CaCl2-Solution

98

Caspase-9 Fluorometric Assay Kit

137

Calcein AM

110, 114

Caspases, active

136

Calcein AM Solution

110, 114

Cathepsin B Activity Assay Kit

138

Calcein, high purity

110, 114

Cathepsin B&L Inhibitor Z-Phe-Phe-FMK

138

Calcium Calibration Buffer Kit

118

Cathepsin B, human (native)

138

Calpain 1, human (native)

138

Cathepsin D Activity Assay Kit

138

Calpain 1 Inhibitor Screening Kit

138

Cathepsin D Inhibitor Screening Kit

138

Calpain Activity Assay Kit

138

Cathepsin D, human (native)

138

Calpain Inhibitor Z-LLY-FMK

138

Cathepsin G Activity Assay Kit

138

cAMP Activity Assay Kit

119

Cathepsin H Activity Assay Kit

138

Camptothecin

141

Cathepsin H, human (native)

138

Camptothecin (2 mM)

142

Cathepsin K Activity Assay Kit

138

Description

Page

Description

Page

Cathepsin L Activity Assay Kit

138

Colorimetric Cell Viability Kit III (XTT)

104

Cathepsin L, human, recombinant

138

Colorimetric Cell Viability Kit IV (MTT)

104

Cathepsin S Activity Assay Kit

138

Colorimetric DNA Fragmentation Detection Kit (IHC)

129

Cathepsin S, human (native)

138

Colorimetric Glutathione Detection Kit

131

CD Antigens, recombinant

179

Colorimetric Glutathione Reductase Activity Kit

131

CD133 Progenitor Cells

53

Colorimetric GST Assay Kit

121

CD14+ Monocytes

54

Colorimetric Nitric Oxide Assay Kit

121

CD34+ Progenitor Cells

52

Complete Cell Fractionation Kit

122

Cell Dissociation Solution ACF

96

Coronary Artery Endothelial Cells

15

Cell Fractionation

122

Coronary Artery Smooth Muscle Cells

38

Cell Lysates

172

Cryo-SFM

79

Cell Pellets

91

Cycloheximide

142

Cell Proliferation Kit I (CFSE)

104

Cycloheximide (100 mM)

142

Cell Transfection

156

Cytochrome c Apoptosis Detection Kit

130

CellClean

165

Cytokine antibodies

169

CFSE; 5(6)-CFDA, SE

110

Cytokine ELISAs

175

cGMP Activity Assay Kit

119

Cytokine Mix E for HPC Expansion

81

Chemokines antibodies

169

Cytokines and Growth Factors

178

Chemokines ELISAs

175

Cytokines, recombinant

179

Chemokines, recombinant

179

Cytosol/Particulate Fractionation Kit

122

Chk2 Inhibitor

43

Cytotoxicity Kits

106

Chondrocyte Media

62

Chondrocytes

12

Chondrogenic Differentiation Medium

80

Chromogenic beta-Galactosidase Substrate Sampler Kit

108

DAPI

109

c-Jun (1-79)-GST Fusion Protein

139

DAPI Solution

109

Classical Media

85

DAPI, dilactate

109

DMEM Media

85

DASPEI

109

DMEM/Hams F-12 Media

85

Daunorubicin.HCl

142

Hams F-10 Media

85

DC Base Media

82

Hams F-12 Media

85

DC Generation Media

82

Leibovitz L-15 Medium

85

Dendritic Cell Media

82

McCoys 5A Media

86

Dermal Blood Endothelial Cells

18

MEM Media

86

Dermal Fibroblasts

27

RPMI 1640 Media

86

Dermal Lymphatic Endothelial Cells

19

Cloning Vectors

166

Dermal Microvascular Endothelial Cells

17

Coelenterazine (native)

108, 116

DetachKit 1/2

95

Coelenterazine 400a

108, 116

Detachment Reagents

95

Coelenterazine cp

108, 116

Dexamethasone

142

Coelenterazine f

108, 116

Dexamethasone (10 mM)

142

Coelenterazine fcp

108, 116

Di-12-ANEPPQ

113

Coelenterazine h

108, 117

Di-2-ANEPEQ (JPW1114)

113

Coelenterazine hcp

108, 116

Di-4-ANEPPS

113

Coelenterazine i

108, 117

Di-8-ANEPPQ

113

Coelenterazine ip

108, 116

Di-8-ANEPPS

113

Coelenterazine n

108, 117

DiA

112

Coelenterazine Sampler Kit

108, 117

DiB

112

Coelenterazine, 2-methyl

108, 117

DiBAC4(3)

113

Colchicine

142

DiD (DiIC18[5])

111

Colorimetric Cell Viability Kit I (WST-8)

104

Dihydrorhodamine 123

109

Colorimetric Cell Viability Kit II (WST-1)

104

Dihydrorhodamine 123, dihydrochloride salt

109

193

Appendix

Alphabetical Index

Appendix

194

Alphabetical Index

Description

Page

Description

Page

DiI (or DiIC18[3])

111

ESC-Qualified Fetal Calf Serum

88

DiI in vegetable oil

111

Ethidium Homodimer I

109, 110

DiIC1(5)

113

Ethidium Homodimer III

109, 110

Dilinoleyl DiI

111

Etoposide

142

Dilinoleyl DiO

111

Etoposide (10 mM)

142

DiO (or DiOC18[3])

111

Expression Vectors

166

DiO/DPA Membrane Potential Detection Kit

113

Extracellular Matrix Proteins

99

DiOC14(3), hydroxyethanesulfonate

111

DiOC16(3)

111

DiOC2(3)

113

DiOC5(3)

113

Fetal Calf Serum

87

DiOC6(3)

109, 110, 113

FFP-18 AM

116

DiR (DiIC18[7])

111

Fibroblast Media

70

Disinfectants

189

Fibroblasts

27

D-Luciferin, Free Acid

108

Fibronectin Solutions

99

D-Luciferin, Potassium Salt

108

FIP-18 AM

116

D-Luciferin, Sodium Salt

108

Flubi-2

118

DMAO

109

Flubida-2

118

DMEM Media

85

Fluo-3 (pentaammonium salt)

115

DMNPE-caged D-Luciferin

108

Fluo-3 (pentapotassium salt)

115

DMSO, anhydrous

114, 118

Fluo-3 (pentasodium salt)

115

DNA Damage Detection Kit

121

Fluo-3 AM

115

DNA Gel Stains

125

Fluo-3 AM Solution

115

DNA Isolation

124

FluoDNA Gel Stain

125

DNA Ladder I (100 bp)

125

FluoProtein Gel Stain

123

DNA Ladder II (1000 bp)

125

Fluorescein-a-bungarotoxin

112

DNA Size Markers

125

Fluorescein-Biotin

114

Doxorubicin.HCl

142

Fluorescein-DHPE

111

DPA

112

Fluorescent Labeling

144

DPA/Terbium for membrane fusion assay

114

Fluorometric Cell Viability Kit I (Resazurin)

104

DPX

114

Fluorometric Cell Viability Kit II (Calcein)

104

Dulbeccos PBS

98

Fluorometric DNA Fragmentation Detection Kit I (BrdU)

129

Fluorometric DNA Fragmentation Detection Kit II (BrdU)

129

Fluorometric DNA Fragmentation Detection Kit III (F-dUTP)

129

Fluorometric Glutathione Detection Kit

131

EB (ethidium bromide) Solution

109, 110

Fluorometric GST Assay Kit

121

ECL Chemiluminescence Detection Kit

174

Fluorometric Nitric Oxide Assay Kit

121

ELISA Development Kits

176

Follicle Dermal Papilla Cell Media

72

ELISAs

175

Follicle Dermal Papilla Cells

30

Endothelial Cell Growth Supplement

99

Freezing Medium

79

Endothelial Cell Growth Supplement/Heparin

99

Fura-2 (pentaammonium salt)

115

Endothelial Cell Media

63

Fura-2 (pentapotassium salt)

115

Endothelial Cell Media MV

64

Fura-2 (pentasodium salt)

115

Endothelial Cells (Large Vessels)

13

Fura-2 AM

115

Endothelial Cells (Microvascular)

17

Fura-2 AM Solution

115

Endothelial Progenitor Medium

82

Fura-2FF (K Salt)

116

Epidermal Keratinocytes

32

Fura-2FF AM

116

Epidermal Melanocytes

33

Furaptra, AM ester

116

Epithelial Cell Media

65

Furaptra, tetrapotassium salt

116

Epithelial Cells

23

Furaptra, tetrasodium salt

116

Description

Page

Description

Page

HCMEC

20

HDBEC

18

G-418 Sulphate Solution

97

HDLEC

19

GelQuick Protein Gel Stain/Destaining Kit

123

HDMEC

17

Gene Cloning and Expression

166

Hematopoietic Progenitor Cell Expansion Medium DXF

81

GeneBooster Reagent I

165

Hematopoietic Progenitor Cell Media

81

GeneBooster Reagent II

165

Hepatocyte Media

73

GeneBooster Reagent III

165

Hepatocytes

31

GeneBooster Reagent Kit

165

HEPES Buffer

98

Gentamicin Solution

97

HEPES Buffered Saline Solution

98

Glutamine, L

99

HFDPC

30

Glutamine, Stable

99

HHpC

31

Green Caspase-12 Staining Kit

134

HMEpC

25

Green Caspase-2 Staining Kit

134

hMNC-CB

57

Green Caspase-3 Staining Kit

134

hMNC-PB

56

Green Caspase-8 Staining Kit

134

hMoCD14+-PB

54

Green Caspase-9 Staining Kit

134

hMo-PB

54

Green Multi-Caspase Staining Kit

134

hMSC-AT

49

Green-beta-D-Gal

108

hMSC-BM

49

Green-fluorescent Cytoplasmic Membrane Staining Kit

111

hMSC-UC

49

Growth Factor antibodies

169

HNEpC

23

Growth Factor ELISAs

175

HOB

34

Growth Factors, recombinant

179

Hoechst 33258

109, 110

Growth Supplements

99

Hoechst 33258 Solution

109, 110

GSK-3a-GST Fusion Protein

139

Hoechst 33342

109, 110

GSK-3b, human, recombinant

139

Hoechst 33342 Solution

109, 110

GST Inhibitor-2 (ethacrynic acid)

121

Hormone ELISAs

175

GST protein (active)

121

Hormones, recombinant

179

HPAEC

16

HPASMC

38

hPC-PL

51

H
Hams F-10 Media

85

HPF

28

Hams F-12 Media

85

HPlEpC

26

Hanks BSS

98

HPMEC

21

HAoAF

28

HPrSMC

41

HAoEC

15

HRCEpC

26

HAoSMC

37

HREpC

25

HAT Activity Assay Kit

121

HSAEpC

24

HAT protein (P/CAF, active)

121

HSaVEC

16

HBdMEC

20

HTEpC

23

HBdSMC

40

HTSMC

39

HBEpC

24

HUAEC

14

HBMEC

22

HUASMC

39

HBSMC

40

HUF

29

HCAEC

15

Human Insulin

99

HCASMC

38

Human Primary Cells

10

hCD133+-CB

53

Cardiac Myocytes

11

hCD34+-CB

52

Chondrocytes

12

HCF

28

Endothelial Cells (Large Vessels)

13

HCH

12

Endothelial Cells (Microvascular)

17

HCM

11

Epithelial Cells

23

195

Appendix

Alphabetical Index

196

Alphabetical Index

Appendix

Description

Page

Description

Page

Fibroblasts

27

Luciferase Reporter Assay Kit I (Firefly)

107

Follicle Dermal Papilla Cells

30

Luciferase Reporter Assay Kit II (Renilla)

107

Hepatocytes

31

Luciferase Reporter Assay Kit III (Firefly & Renilla)

107

Keratinocytes

32

Luciferase Reporter HTS Kit (Firefly)

107

Melanocytes

33

Lymphatic Endothelial Cells

19

Osteoblasts

34

Lymphocyte Separation Medium 1077

84

Preadipocytes

35

Skeletal Muscle Cells

36

Smooth Muscle Cells

37

Human Stem and Blood Cells

48

M1-Macrophage Generation Medium DXF

83

Human Transferrin

99

M2-Macrophage Generation Medium DXF

83

HUtMEC

21

MA Lipofection Enhancer

164

HUtSMC

41

Macrophage Base Medium DXF

83

HUVEC

13

Macrophage Detachment Solution DXF

96

HVMF

29

Macrophage Generation Media DXF

83

HWP

35

Magnet Plate

164

Hydroxystilbamidine

114

Magnetic Separator (re-usable)

141

Hygromycin B Solution

97

Mammalian Whole Cell Protein Extraction Kit

122

Mammary Epithelial Cell Media

67

Mammary Epithelial Cells

25

MATra-A Reagent

164

I, J, K, L
Indo-1 (pentaammonium salt)

115

MATra-S Immobilizer

164

Indo-1 (pentapotassium salt)

115

MATra-siRNA Reagent

164

Indo-1 (pentasodium salt)

115

McCoys 5A Media

86

Indo-1 AM

115

Media and Sera

60

Indo-1FF (K+ Salt)

116

Media for Primary Cells

61

Indo-1FF AM

116

Chondrocyte Media

61

Insect Cell Culture Media

87

Endothelial Cell Media

63

Insulin

99

Endothelial Progenitor Medium

82

Interleukin antibodies

169

Epithelial Cell Media

65

Interleukin ELISAs

175

Fibroblast Media

70

Interleukins, recombinant

179

Follicle Dermal Papilla Cell Media

72

Ionomycin (calcium salt)

118, 142

Hematopoietic Progenitor Cell Media

81

Ionomycin (free acid)

118, 142

Hepatocyte Media

73

JC-1 chloride

110

Keratinocyte Media

74

JC-1 iodide

110

Melanocyte Media

75

JNK Activated Cell Lysate

139

Mesenchymal Stem Cell Differentiation Media

80

JNK Activity Detection Kit

139

Mesenchymal Stem Cell Growth Medium

80

JNK Activity Screening Kit

139

Mesenchymal Stem Cell Growth Medium DXF

80

JNK Negative Control Cell Lysate

139

Mesenchymal Stem Cell Media

80

Keratinocyte Media

74

Monocyte Attachment Medium

83

Keratinocytes

32

Mononuclear Cell Media

84

LDH Cytotoxicity Kit II

106

Myocyte Media

62

Leibovitz L-15 Medium

85

Osteoblast Media

76

L-Glutamine Solution

99

Pericyte Media

81

Live/Dead Cell Staining Kit II

105

Preadipocyte/Adipocyte Media

77

L-JNK Peptide Inhibitor I

139

Skeletal Muscle Cell Media

78

L-JNKI1 Negative Control Peptide

139

Smooth Muscle Cell Media

79

Lucifer Yellow Cadaverine

114

Melanocyte Media

75

Lucifer Yellow CH lithium salt

114

Melanocytes

33

Lucifer Yellow CH potassium salt

114

MEM Alpha Media

86

Description

Page

Description

Page

MEM Amino Acids Solution

99

Nerve Terminal Red Staining Kit II

112

MEM D-Valine, w/o L-Glutamine

86

Neuro-DiI

111

MEM Media

86

Neuro-DiO

111

MEM NEAA Solution

99

Neurogenic Differentiation Medium

80

MEM Vitamin Solution

99

NHDF

27

Membrane Protein Extraction Kit

122

NHEK

32

Mesenchymal Stem Cell Adipogenic Differentiation Medium

80

NHEM

33

Mesenchymal Stem Cell Chondrogenic Differentiation Medium

80

Nonyl Acridine Orange (or NAO)

110

Mesenchymal Stem Cell Growth Medium

80

Nuclear/Cytosol Fractionation Kit

122

Mesenchymal Stem Cell Neurogenic Differentiation Medium

80

Nucleic Acid Isolation

124

Mesenchymal Stem Cell Osteogenic Differentiation Medium

80

Octadecyl Rhodamine B Chloride (R18)

112

Mesenchymal Stem Cells

49

Okadaic acid

142

Mesenchymal Stem Cells from Adipose Tissue

49

Orange-fluorescent Cytoplasmic Membrane Staining Kit

111

Mesenchymal Stem Cells from Bone Marrow

49

Ordering and Technical Support

205

Mesenchymal Stem Cells from Umbilical Cord Matrix

49

Osteoblast Media

76

Microbial Detection and Elimination

184

Osteoblast Mineralization Medium

76

Mineralization Medium Osteoblasts

76

Osteoblasts

34

Mitochondria/Cytosol Fractionation Kit

122

Osteogenic Differentiation Medium

80

Mitochondrial Apoptosis Staining Kit

130

Oxidized Glutathione (GSSG)

121

Mitochondrial DNA Isolation Kit

124

MitoGreen

110

Mitomycin C

142

MitoRed

110

PBS

98

Mitsuhashi/Maramorosh Insect Medium

87

PCR Bacteria Test Kit

188

Monocyte Attachment Medium

83

PCR Mycoplasma Test Kit I/C

186

Monocytes

54

PCR Mycoplasma Test Kit I/RT; Variant A

186

Mononuclear Cell Medium

84

PCR Mycoplasma Test Kit I/RT; Variant B

186

Mononuclear Cells

56

PCR Mycoplasma Test Kit III

186

Mononuclear Cells from Cord Blood

57

PCR Purification Kit

124

Mononuclear Cells from Peripheral Blood

56

Pellets

91

MQAE

117

Pen/Strep Solution

97

MSC-Qualified Fetal Calf Serum

88

Pen/Strep/Fungizone Solution

97

Mycoplasma Disinfection Kit I

189

Pericyte Media

81

Mycoplasma Disinfection Kit II

189

Pericytes

51

Mycoplasma-EX

187

Pharmacidal Spray

189

Mycoplasma-EX Spray

189

Phorbol-12-myristate 13-acetate (PMA)

142

Myocyte Media

62

Phycobiliprotein Dyes

152

Placental Epithelial Cell Media

69

Placental Epithelial Cells

26

Plasmid Vectors

166

N, O

NAD/NADH Quantitation Kit

121

Pluronic F-127

114, 118

NADP/NADPH Quantitation Kit

121

Pluronic F-127, 10% in H2O

114, 118

Nasal Epithelial Cells

23

Pluronic F-127, 20% solution in DMSO

114, 118

NBD C6-ceramide

112

pPK-CMV-E1 Expression Vector Kit

166

NBD C6-sphingomyelin

112

pPK-CMV-E2 Expression Vector Kit

166

NBD-DHPE

111

pPK-CMV-E3 Expression Vector Kit

166

Nerve Terminal Green Staining Kit I

112

pPK-CMV-F1 Fusion Vector (C-GFP)

166

Nerve Terminal Green Staining Kit II

112

pPK-CMV-F2 Fusion Vector (N-GFP)

166

Nerve Terminal Green Staining Kit III

112

pPK-CMV-F3 Fusion Vector (C-Luc)

166

Nerve Terminal Green Staining Kit IV

112

pPK-CMV-F4 Fusion Vector (N-Luc)

166

Nerve Terminal Red Staining Kit I

112

pPK-CMV-R0 Reporter Vector (Blank)

166

197

Appendix

Alphabetical Index

Appendix

198

Alphabetical Index

Description

Page

Description

Page

pPK-CMV-R1 Reporter Vector (b-Gal)

166

PromoFluor-XXX-Goat Anti-Rabbit IgG

150, 173

pPK-CMV-R2 Reporter Vector (CAT)

166

PromoFluor-XXX-Labeling-Kit

148

pPK-CMV-R3 Reporter Vector (Luc)

166

Propidium Iodide (PI)

109, 110

pPK-CMV-R4 Reporter Vector (GFP)

166

Propidium Iodide (PI) Solution

109, 110

pPK-CMV-R5 Reporter Vector (SEAP)

166

Prostate Smooth Muscle Cells

41

Preadipocyte Media

77

Protein and Antibody Labeling Kits

148, 174

Preadipocytes

35

Protein Extraction

122

Prestained Protein Ladder I

123

Protein Gel Stains

123

Prestained Protein Ladder II

123

Protein Size Markers

123

Primary Cells

10

Pulmonary Artery Endothelial Cells

16

PromoFectin

160

Pulmonary Artery Smooth Muscle Cells

38

PromoFectin-CellLine

160

Pulmonary Fibroblasts

28

PromoFectin-Hepatocyte

160

Pulmonary Microvascular Endothelial Cells

21

PromoFectin-HUVEC

160

Purple-beta-D-Gal

108

PromoFectin-Insect

160

PromoFectin-Macrophage

160

PromoFectin-Neuron

160

PromoFectin-Polypeptide

160

Rapamycin

142

PromoFectin-siRNA

160

Reagents and Supplements

94

PromoFluor Amino-Modified Label

147

Receptors, recombinant

179

PromoFluor Antifade Reagent

153

Red Caspase-3 Staining Kit

134

PromoFluor Carboxylic Acid

147

Red Caspase-8 Staining Kit

134

PromoFluor ChipPack

147

Red Caspase-9 Staining Kit

134

PromoFluor Maleimide

147

Red Multi-Caspase Staining Kit

134

PromoFluor NHS-Ester

147

Red-beta-D-Gal

108

PromoFluor-XXX-Goat Anti-Mouse IgG

150

Red-fluorescent Cytoplasmic Membrane Staining Kit

111

PromoFluor-430 Nick Translation Labeling Kit

149

Reduced Glutathione (GSSG)

121

PromoFluor-430 PCR Labeling Kit

149

Renal Cortical Epithelial Cells

26

PromoFluor-430-dUTP (NT)

151

Renal Epithelial Cell Media

68

PromoFluor-430-dUTP (PCR)

151

Renal Epithelial Cells

25

PromoFluor-500 Nick Translation Labeling Kit

149

Reporter Assays

107

PromoFluor-500 PCR Labeling Kit

149

Reporter Vectors

166

PromoFluor-500-dUTP (NT)

151

Resazurin, sodium salt

104

PromoFluor-500-dUTP (PCR)

151

Resistin EDK, human

105

PromoFluor-550 Nick Translation Labeling Kit

149

RH237

113

PromoFluor-550 PCR Labeling Kit

149

RH414

113

PromoFluor-550-dUTP (NT)

151

RH421

113

PromoFluor-550-dUTP (PCR)

151

RH795

113

PromoFluor-594 Nick Translation Labeling Kit

149

Rhod-2 (triammonium salt)

115

PromoFluor-594 PCR Labeling Kit

149

Rhod-2 (tripotassium salt)

116

PromoFluor-594-dUTP (NT)

151

Rhod-2 (trisodium salt)

116

PromoFluor-594-dUTP (PCR)

151

Rhod-2 AM

116

PromoFluor-640 Nick Translation Labeling Kit

149

Rhodamine 123

110

PromoFluor-640 PCR Labeling Kit

149

Rhodamine-DHPE

111

PromoFluor-640-dUTP (NT)

151

Rose-beta-D-Gal

108

PromoFluor-640-dUTP (PCR)

151

Rosiglitazone

142

PromoFluor-XXX, phalloidin conjugate

150

R-Phycoerythrin

152

PromoFluor-XXX, streptavidin conjugate

152

RPMI 1640 Media

85

PromoFluor-XXX. biotin conjugate

152

RTU DNA Ladder I

125

PromoFluor-XXX-aadUTP

151

RTU DNA Ladder II

125

PromoFluor-XXX-Goat Anti-Human IgG

150, 173

RuBP-2S

114

Description

Page

Description

Page

RuBP-4S

114

Thapsigargin

142

RuBPS

114

TMRE

110, 113

TMRM

110, 113

TPEN

117

Trace Element Mix

99

S
Saphenous Vein Endothelial Cells

16

Tracheal Epithelial Cells

23

SCAS

112

Tracheal Smooth Muscle Cells

39

Schneiders Drosophila Medium

87

Transfection Reagents

156

SDIP/Europium for membrane fusion assay

114

Transferrin

99

Senescence Detection Kit

106

TRITC-DHPE

111

Sera

87

Trypan Blue Solution (0.5%)

110

Set of four Magnet Bar Plates

164

Trypsin Neutralizing Solution (TNS)

96

Skeletal Muscle Cell Media

78

Trypsin Solutions

96

Skeletal Muscle Cells

36

Tyrphostin AG 1295

142

SkMC

36

Small Airway Epithelial Cell Media

66

Small Airway Epithelial Cells

24

Smooth Muscle Cell Media

79

Umbilical Artery Endothelial Cells

14

Smooth Muscle Cells

37

Umbilical Artery Smooth Muscle Cells

39

SOD Assay Kit

121

Umbilical Vein Endothelial Cells

13

Sodium bicarbonate Solution

98

Uterine Fibroblasts

29

Soluble Receptors, recombinant

179

Uterine Microvascular Endothelial Cells

21

Stable Glutamine Solution

99

Uterine Smooth Muscle Cells

41

Staurosporine

142

Villous Mesenchymal Fibroblasts

29

Stem and Blood Cells

48

Western Blotting Kits and Reagents

174

Stem Cell Research

170, 181

White Preadipocytes

35

Sulforhodamine 101

114

X-Gal

108

Sulforhodamine 101-a-bungarotoxin

112

Zinquin, ethyl ester

117

Sulforhodamine B

114

Zinquin, free acid

117

Sulforhodamine G

114

Superoxide Dismutase (SOD, human)

121

Supplements

99

SynapseGreen C1

112

SynapseGreen C18 (FM3-25)

112

SynapseGreen C2 (FM2-10)

112

SynapseGreen C3

113

SynapseGreen C4 (FM1-43)

113

SynapseGreen C5 (FM1-84)

113

SynapseRed C1 (FM4-64)

113

SynapseRed C2 (FM4-64)

113

SynapseRed C2M (FM5-95)

113

T
Tamoxifen citrate

142

TC Water

98

Tetrabromorhodamine 123, bromide

110

Tetramethylrhodamine-a-Bungarotoxin

113

Texas Red-DHPE

111

TexasRed PCR Labeling Kit

149

TexasRed-dUTP (PCR)

151

U, V, W, X, Z

199

Appendix

Alphabetical Index

200

International Distributors

International Distributors
Cat. Number
Albania

Page
Biomedica
Shpk
Zona 4, Godina 1
Kati 1, Ap. 1
1000 Tirana
Albania

Cat. Number

Product
Phone:

Page

Fax:
Email:
Internet:

+355-69 40 91 613
+355-45 00 681
a.selimi@bmgrp.al
www.biomedica.co.at

Banksia Scientific Company


3/160 Lytton Road
PO Box 529
Bulimba QLD 4171
Australia

Phone:
Phone:
Fax:
Email:
Internet:

1300 769944 (toll-free)


+61-7-39 02 30 00
+61-7-32 17 98 69
sales@banksiascientific.com.au
www.banksiascientific.com.au

Life Research Pty Ltd.


1 Dalmore Drive, Caribbean Park
Scoresby, Victoria 3179
Australia

Phone:
Fax:
Email:
Internet:

+61-3-97 64 25 66
+61-3-86 48 58 41
info@liferesearch.com
www.liferesearch.com

Austria

Biomedica Medizinprodukte GmbH & Co KG


Divischgasse 4
1210 Wien
Austria

Phone:
Fax:
Email:
Internet:

+43-1-29 10 75 6
+43-1-29 01 42 9
sales@bmgrp.at
www.biomedica.co.at

Bangladesh

KRISHGEN BioSystems
Unit Nos#318/319
3rd Floor
Shah & Nahar Ind. Premises
Dr E Moses Road
Worli
Mumbai 400018
India

Phone:
Fax:
Email:
Internet:

+91-22-66 37 29 90
+91-22-66 37 29 91
sales@krishgen.com
www.krishgen.com

Bosnia-Herzegovina

Biomedica d.o.o.
Zmaja od Bosne 4
71000 Sarajevo
Bosnia

Mobile:
Phone:
Fax:
Email:
Internet:

+387-66 90 60 81
+387-33 26 27 25
+387-33 26 27 26
biomedica@bmgrp.ba
www.biomedica.co.at

Bulgaria

Biomedica Bulgaria Ltd.


Sopharma Business Towers
5 Lachezar Stanchev Str.
Bl. B, Office 4-B6, Fl. 6
1766 Sofia
Bulgaria

Phone:
Fax:
Email:
Internet:

+359-2-44 72 833
+359-2-44 72 831
officeBg@bmgrp.eu
www.biomedica-bg.eu

Canada

CEDARLANE
4410 Paletta Court
Burlington, Ontario, Canada
L7L 5R2
Canada

Phone:
Fax:
Email:
Internet:

1-800-268-5058 (toll-free)
+1 289-288-0020
general@cedarlanelabs.com
www.cedarlanelabs.com

MJS BioLynx Inc.


300 Laurier Blvd.
Brockville, Ontario, Canada
K6V 5W1
Canada

Phone:
Fax:
Email:
Internet:

1-888-593-5969 (toll-free)
+1 613 342-1341
sales@biolynx.ca
www.biolynx.ca

China

Jiangsu Pu Nuo Sheng (PromoCell) Biotech Ltd.


Building 2, No.6 Dong Sheng Xi Road
National High Tech Development Park
Jiangyin, 214434
China

Phone:
+86-510-81 69 51 00
Fax:
+86-510-81 69 52 00
Email: info@promocell-china.com
Internet:
www.promocell-china.com

Croatia

Biomedica Dijagnostika d.o.o.


Paljetkova 2
10000 Zagreb
Croatia

Phone:
Fax:
Email:
Internet:

+385 1 888 5727


+385 1 888 5728
biomedica-dijagnostika@bmgrp.hr
www.biomedica.co.at

Czech Republic

BIOMEDICA CS, s.r.o.


Meteor Centre Office Park B
Sokolovsk 100/94
186 00 Praha 8
Czech Republic

Phone:
Phone:
Fax:
Email:
Internet:

+420 283 931 485


+420 545 214 915
+420 283 932 507
info@biomedica.cz
www.biomedica.cz

Denmark

PromoCell GmbH
Sickingenstr. 63/65
69126 Heidelberg
Germany

Phone:
+49 6221 - 649 34 0
Fax:
+49 6221 - 649 34 40
Email: lorna.whyte@promocell.com
Internet:
www.promocell.com

Finland

Biotop Oy
Tykistkatu 6B 3
20520 Turku
Finland

Phone:
Fax:
Email:
Internet:

+358-2-241 00 99
+358-2-241 01 11
info@biotop.fi
www.biotop.fi

Hungary

Biomedica Hungria Kft.


Ganz u. 16
1027 Budapest
Hungary

Phone:
Fax:
Email:
Internet:

+361-22 53 85 0
+361-20 12 68 4
biomed@biomedica.hu
www.biomedica.hu

Australia

Appendix

Product

India

DSS Takara Bio India Pvt. Ltd.


A-5 Mohan Co-op Indl. Estate
Mathura Road
New Delhi-110044
India

Phone:
Fax:
Email:
Email:

+91-33-30886717/21
+91-269593821
orderinfo@dsstakarabio.com
orderinfo@dssimage.com

KRISHGEN BioSystems
Unit Nos#318/319, 3rd Floor
Shah & Nahar Ind. Premises
Dr E Moses Road
Worli
Mumbai 400018
India

Phone:
Fax:
Email:
Internet:

+91-22-66 37 29 90
+91-22-66 37 29 91
sales@krishgen.com
www.krishgen.com

Indonesia

KRISHGEN BioSystems
Unit Nos#318/319, 3rd Floor
Shah & Nahar Ind. Premises
Dr E Moses Road
Worli
Mumbai 400018
India

Phone:
Fax:
Email:
Internet:

+91-22-66 37 29 90
+91-22-66 37 29 91
sales@krishgen.com
www.krishgen.com

Israel

Biological Industries Ltd.


Kibbutz Beit Haemek, 25115
Israel

Phone:
Fax:
Email:
Internet:

+972-4-996-0595
+972-4-996-0631
info@bioind.com
www.bioind.com

Italy

VWR International PBI S.r.l.


Via San Giusto, 85
20153 Milano
Italy

Phone:
Fax:
Email:
Internet:

+39-02-48 77 91
+39-02-40 09 00 10
info@it.vwr.com
it.vwr.com

Japan

Funakoshi Co. Ltd.


9-7 Hongo 2-chome
Bunkyo-ku
Tokyo
Japan 113-0033

Phone:
Fax:
Email:
Internet:

+81-3-56 84 16 20
+81-3-56 84 17 75
reagent@funakoshi.co.jp
www.funakoshi.co.jp

TaKaRa Bio Inc.


Seta 3-4-1
Otsu, Shiga 520-2193
Japan

Phone:
Fax:
Internet:

+81-77-543 7247
+81-77-543 9254
www.takara-bio.co.jp

Korea

Sungwoo Life Science Co., Ltd.


Samsung BLDG 4th Floor 560-5
Uijeongbu 2Dong Uijeongbu-City
Kyeonggi-Do
South Korea

Phone:
Fax:
Email:
Internet:

+82-2-985-7471
+82-31-876-9149
sungwoo@sungwools.com
www.sungwools.com

Latvia

SIA DIAMET
Aizkraukles iela 23
Riga, 1006
Latvia

Phone:
Fax:
Email:
Internet:

+371 26 47 47 64
+371 67 54 33 31
diamet@diamet.lv
www.diamet.lv

Macedonia

Biomedika Dijagnostika dooel


Ul. llindenska br. 103 lokal 2 i 3
1000 Skopje
Macedonia

Phone:
Fax:
Email:
Internet:

+389 703 62 676


+389 232 22 404
v.peeva@bmgrp.mk
www.biomedica.co.at

Malaysia

Biomarketing Services SDN BHD


21, Jalan 4/62A
Bandar Menjalara, Kepong
52200 Kuala Lumpur
Malaysia

Phone:
Fax:
Email:
Internet:

+603 6273 3068


+603 6272 0093
info@biomarketing.com.my
www.biomarketing.com.my

Nepal

KRISHGEN BioSystems
Unit Nos#318/319, 3rd Floor
Shah & Nahar Ind. Premises
Dr E Moses Road
Worli
Mumbai 400018
India

Phone:
Fax:
Email:
Internet:

+91-22-66 37 29 90
+91-22-66 37 29 91
sales@krishgen.com
www.krishgen.com

Netherlands

Bio-Connect B.V.
Begonialaan 3a
6851 TE Huissen
Netherlands

Phone:
Fax:
Email:
Internet:

+3126 326 4450


+3126 326 4451
info@bio-connect.nl
www.bio-connect.nl

New Zealand

Banksia Scientific Company


12 Brigade Street
Airport Oaks
Auckland
New Zealand

Phone:
Fax:
Email:
Internet:

+64-9-921 26 26
+64-9-921 26 27
sales@banksiascientific.co.nz
www.banksiascientific.co.nz

Life Research Pty Ltd.


Level 1, 5 Lakeside Drive
Burwood East Victoria 3151
Australia

Phone:
Fax:
Email:
Internet:

+61-3-98 03 00 45
+61-3-86 48 58 41
info@liferesearch.com
www.liferesearch.com

201

Appendix

International Distributors

Appendix

202

International Distributors

Norway

PromoCell GmbH
Sickingenstr. 63/65
69126 Heidelberg
Germany

Phone:
+49 6221 - 649 34 0
Fax:
+49 6221 - 649 34 40
Email: lorna.whyte@promocell.com
Internet:
www.promocell.com

Poland

Biomedica Poland sp. z.o.o.


Ul. Raszyska 13
05-500 Piaseczno
Poland

Phone:
Fax:
Email:
Internet:

+48-22 73 75 996
+48-22 73 75 994
biomedica@bmgrp.pl
www.biomedica.pl

Portugal

LABCLINICS, S.A.
Industria, 54
08025 Barcelona
Spain

Phone:
Fax:
Email:
Internet:

+34-93-446 47 00
+34-93-348 10 39
info@labclinics.com
www.labclinics.com

Romania

S.C.C.Y.B.E.R. Srl Romania


Str. Smaranda Braescu, nr. 20D
Sector 1, Cod 014203
70941 Bucharest
Romania

Phone:
Phone:
Fax:
Email:
Internet:

+40-37 27 66 844
+40-37 27 66 845
+40-21 23 22 204
gabriela.matei@cybermed.ro
www.cyber-srl.ro

Serbia

BIOMEDICA MP d.o.o.
Cara Dusana 214
11080 Zemun
Serbia

Phone:
Fax:
Email:
Internet:

+381 11 6301 882


+381 11 6301 883
office@biomedicamp.com
www.biomedica.co.at

Singapore

Biomed Diagnostics Pte. Ltd.


18 Boon Lay Way
#05-105-108 TradeHub 21
Singapore 609966

Phone:
Fax:
Email:
Internet:

+65-6-298 43 47
+65-6-298 47 23
sales@biomed.com.sg
http://www.biomed.com.sg/main.htm

Singlab Technologies Pte. Ltd.


1003 Bukit Merah Central #07-50
Inno. Centre
Singapore 159836

Phone:
Fax:
Email:
Internet:

+65-6-272 07 88
+65-6-270 71 28
PromoCell@singlab.com.sg
www.singlab.com.sg

Slovakia

Biomedica Slovakia s.r.o.


Drobnho 27
84101 Bratislava
Slovakia

Phone:
Fax:
Email:
Internet:

+421-2-69 30 99 01
+421-2-69 30 99 08
biomedica@biomedica.sk
www.biomedica.sk

Slovenia

Biomedis M.B.
Slokanova ulica 12
2000 Maribor
Slovenia

Phone:
Fax:
Email:
Internet:

+386-2-47 16 300
+386-2-47 16 304
info@biomedis-mb.si
www.biomedis-mb.si

South Korea

Sungwoo Life Science Co., Ltd.


Samsung BLDG 4th Floor 560-5
Uijeongbu 2Dong Uijeongbu-City
Kyeonggi-Do
South Korea

Phone:
Fax:
Email:
Internet:

+82-2-985-7471
+82-31-876-9149
sungwoo@sungwools.com
www.sungwools.com

Spain

LABCLINICS, S.A.
Industria, 54
08025 Barcelona
Spain

Phone:
Fax:
Email:
Internet:

+34-93-446 47 00
+34-93-348 10 39
info@labclinics.com
www.labclinics.com

Sri Lanka

KRISHGEN BioSystems
Unit Nos#318/319
3rd Floor
Shah & Nahar Ind. Premises
Dr E Moses Road
Worli
Mumbai 400018
India

Phone:
Fax:
Email:
Internet:

+91-22-66 37 29 90
+91-22-66 37 29 91
sales@krishgen.com
www.krishgen.com

Sweden

Mediqip AB
Frrdsvgen 10
141 46 Huddinge
Sweden

Phone:
Fax:
Email:
Internet:

+46-8-448 10 10
+46-8-448 10 75
info@mediqip.se
www.mediqip.se

Taiwan

Biochiefdom International Co. Ltd.


1F, No 50, Yishou St.
Wenshan District
Taipei 11659
Taiwan

Phone:
Fax:
Email:
Internet:

+886-2-29 37 00 88
+886-2-29 37 97 17
info@bio-chief.com.tw
www.bio-chief.com.tw

Thailand

Biomed Diagnostics (Thailand) Co. Ltd.


1518/4 Kengrungruengchai Tower, 7th Floor
Wong Sawang, Bang Sue,
Bangkok 10800
Thailand

Phone:
Fax:
Email:
Internet:

+66-2-879 60 26
+66-2-879 60 65
nan@biomedthai.com
www.biomedthai.com

For an up-to-date list of all PromoCell and PromoKine distributors, please see www.promocell.com/contact

Terms and Conditions

203

General Terms

Delivery

1. These conditions of sale and delivery


apply to all contracts of supply between
us and our customers, even when in individual cases they are not referred to.
2. A customers inconsistent conditions
of purchase shall have no validity, even
when we do not expressly reject such
conditions.
3. The customer is deemed to agree to
these conditions of sale and delivery
upon unconditional acceptance of the
goods supplied.
4. Rights of the customer arising during
the course of business with us are not
transferable.
5. Any agreement that differs from these
conditions of sale and delivery, or any
recognition of the customers contradictory purchase conditions, requires our
express confirmation in writing.

1. Delivery times will be specified after


careful co-ordination. They are however
not legally binding. Claims for compensation due to delayed or non-delivery are
excluded.
2. If a delivery date specified by us is
exceeded by more than two weeks, the
customer is entitled to give us a further
two week time limit for delivery. Should
the delivery not be carried out by the
end of the additional two week delivery
period, the customer is entitled to rescind the contract. The rescission must
be made promptly and in writing, at the
latest within a week of the expiry of the
additional two week delivery period.
3. All deliveries take place at the customers risk. The passing of risk takes place
on the handing over of the goods to the
carrier or haulier. The careful choice of
dispatch method and means of transport
is at our discretion with exclusion of any
and all liability.
4. Deliveries in part are permitted. They
are to be regarded as separate deliveries.

Offers
1. In the absence of a declaration to the
contrary, we are not bound by any offers
we make.
2. Supply contracts only take effect when
confirmed by us in writing.
3. Any inquiries or complaints concerning
confirmation are to be made promptly in
writing.
4. Assurances and assured properties
only exist when they are expressly described as such. The descriptions and
other statements in our catalogues and
price lists are accurate to the best of our
knowledge and belief. Under no circumstances may any assurances as to properties be derived from such descriptions
and statements.

Prices
1. Our prices quoted in the current price
list are net prices, VAT (value added tax)
not included.
2. VAT is charged according to the applicable laws in effect.
3. Charges for delivery, cooling and insurance are indicated in the valid price
list.
4. PromoCell prices are subject to change
without prior notice.

Payment
1. Payment is to be made without any
deductions within 14 days of delivery
and remittance of the bill.
2. Default of payment will result in legal
proceedings upon the expiry of the payment period without further warning. In
this case, or in the case of a warning, we
are entitled to interest for late payment
at the current normal bank borrowing
rate, or alternatively at a rate of 5%
above the current discount rate of the
Deutsche Bundesbank.
3. A cash discount is only permitted
when expressly stated on the bill and
when payment is made within the stated
period.
4. An offset against or demand for
payment is only permitted when the
customer has an undisputed or legally
established counterclaim.
5. If our conditions of payment are not
complied with or if justifiable doubts
exist regarding the customers ability or
willingness to pay, we are entitled to

rescind the contract and to demand the


return of the delivered goods, the costs
of delivery to be borne by the customer.
In addition, all time outstanding claims
will become due.
Justifiable doubts referred to above
include, but are not restricted to: unfavourable information, deterioration of
financial circumstances, initiation of insolvency or bankruptcy proceedings, execution measures against the customer,
protest of bills, and default of payment
for any other reason.
6. If the customer does not take delivery of properly delivered goods, we are
entitled, after setting an additional time
limit, to rescind the contract, or to damages for non-compliance with the contract. In the latter case we are entitled to
demand either damages of 25% of the
sales price without proof, or the actual
amount of damages.

Complaint
1. Any complaint regarding the goods,
such as defects, lack of assured properties, wrong delivery and over- or underdelivery are, after immediate discontinuation of use, to be reported in writing.
2. In the case of wrong delivery, the
goods will be taken back at our expense
and replaced with the goods ordered.
Excess deliveries may similarly be sent
back to us at our expense. In the case of
under delivery, the customer may choose
between a reduction in payment or complete fulfilment of the order.
3. In the case of delivery of faulty goods
we are entitled to replace the faulty
goods with goods free of faults. Should
the replacement delivery also be faulty,
the customer may choose between a
reduction in payment or cancellation of
the contract. Claims for compensation
are excluded except in the case of lack of
assured properties.
4. The right to complain expires in the
case of obvious or apparent defects two
weeks after receipt of the goods, and
in the case of non-apparent defects six
months after receipt of the goods.
5. Goods may only be returned by arrangement with us and with the issue
of a return number. The goods must be

Appendix

Terms and Conditions

Appendix

204

Terms and Conditions

properly packed. If returned goods are


damaged due to the fault of the customer, the customer is obliged to pay
compensation for such damage.
6. In the case of delivery to traders the
customer is to inspect the goods without delay upon receipt and to report
any complaints to us immediately. If the
customer fails to make such a report
the goods will be deemed approved. In
the case of a fault that is not apparent
upon inspection, the complaint must be
reported immediately upon discovery of
the fault, otherwise the goods will be
deemed approved as regards such fault.

Use of Goods
1. Goods delivered by us are intended
exclusively for the purposes of research
and may not be used on human beings,
animals, in the household, or for any
private use. We emphasise that the use
of our products for diagnostic or therapeutic purposes is subject to current legal
regulations.
2. We do not accept any liability for the
improper use of our products. We deliver
only to commercial enterprises, resalers
and public research, development and
teaching establishments. Legal regulations require us to carefully check orders
and to refuse delivery when there are
signs of improper use of our products.
Orders from students or other private
individuals must be given from an official
office of their institute or firm.
3. We expressly oppose research or any
uses which run counter to ethical, legal
or political standards or principles within
Germany and/or the import country, or
whose ethical justification is the subject
of public debate and dispute in the respective country (e.g. certain cloning
experiments).
We do not accept any liability for such
research or use by our customers, or
by third parties who have received our
products from our customers.

Liability
1. All products delivered by us are only
to be handled by trained personnel who
are well aware of any potential dangers.
Should any liability for compensation

arise as a result of damage caused by


breach of this condition, the customer
shall be liable to pay any such compensation. In all cases, current safety regulations are to be observed, especially in the
case of cells of human or animal origin,
which always represent a potential risk
of infection. The lack of a danger symbol
does not mean that the substances concerned are harmless.
2. We accept no responsibility for damage, loss, or injury caused by incorrect
handling or use in the household, on
humans, or on animals. In as far as we
have made confirmation of delivery dependent upon a specified use of certain
products, the customer accepts full liability for any damage suffered by us as
a result of the customers incorrect use
of the product. In the case of products
which may only be used in accordance
with legal or official regulations, the customers order simultaneously counts as
a declaration that the products will be
used for a specific purpose in the above
sense.
3. Our customers are required without
exception to apply the laboratory guidelines of the professional association of
the chemical industry when dealing with
our products.
4. Passing on of our products to private
individuals is not permitted. If as a result
of a breach of this condition for whatever reason we should receive a claim for
compensation from a private individual,
we shall have a right of redress against
the customer.
5. PromoCell provides no warranties as
to the accuracy of the information contained in printed materials like product
informations, catalogs, instruction manuals, etc. and shall bear no responsibility
or liability whatsoever for any errors,
faults or omissions in the information.

ers or employees. We accept no liability


for damage caused by the behaviour of
companies or individuals which sell our
products.
3. The sale of our products under their
trade names for further commercial use
does not mean that these products and
their trade names may be freely used
in general circulation. Some products,
their production and use or their trade
names are patented or protected trade
names. The further commercial use of
such products and their trade names occurs as regards a third party under the
exclusive responsibility and liability of
the customer.

Indemnification

Managing Director: Dirk Httner


AG Mannheim HRB 335480

1. In addition to section complaint,


claims by the customer are excluded, in
so far as they are not based upon intention or gross negligence.
2. In the case of delivery to a trader this
exclusion also applies to any damage
caused by the intention or gross negligence of our business associates, carri-

Miscellaneous
1. German law shall apply to all contracts
entered into by us, including those for
deliveries abroad. We expressly reject the
application of the Vienna UN-agreement
relating to international sales contracts
of 11. 04. 80.
2. In the case of contracts with traders
who do not belong to the traders specified in 4 of the code of commercial law,
the place for fulfilment of contracts and
the court of jurisdiction shall be Heidelberg.
3. If any of the above conditions is held
to be invalid, the validity of any of the
other conditions shall not be affected.
4. Only the German version of this Terms
of Delivery and Payment is valid. This
translation shall only serve as assistance
for our customers. You can get a German
version on request.

PromoCell GmbH
Sickingenstr. 63/65
69126 Heidelberg
Germany

Ordering and Technical Support

205

Ordering and Technical Support


Ordering Information

Phone

Fax

Online

Our customer service representatives are


ready to assist you with ordering Monday
through Friday

Please copy the fax order form on the last


page and send it to the following fax numbers:

USA/Canada
1 866 251 2860 (toll free)

USA/Canada
1 866 827 9219 (toll free)

Online ordering is available 24 hours/day,


7 days per week. Our online ordering system is secure, fast, and convenient.
Check out www.promocell.com or www.
promokine.info for more information.

Germany
0800 776 66 23 (toll free)

Germany
0800 100 83 06 (toll free)

France
0800 90 93 32 (toll free)

France
0800 90 27 36 (toll free)

United Kingdom
0800 96 03 33 (toll free)

United Kingdom
0800 169 85 54 (toll free)

Switzerland
+49 6221 649 34 0

Switzerland
+49 6221 649 34 40

Netherlands
+49 6221 649 34 0

Netherlands
+49 6221 649 34 40

Email
Please send an e-mail to
order@promocell.com and include the
following information:
Catalog number, name, size and quantity
Shipping and billing address
Contact person`s name and telephone
number

Other Countries
Please see www.promocell.com/distributor or call +49 6221 649 34 0

PromoCell GmbH directly supplies to Belgium, Canada, France, Germany, Ireland,


Liechtenstein, Luxembourg, Monaco,
Netherlands, Switzerland, United Kingdom, and the United States.
All deliveries are carried out using our
recommended carrier, unless an alternative agreement has been arranged
with the customer. Delivery time and
shipment costs are extremely favorable. Our local sales representatives
and our multilingual customer service
are looking forward to serving you.
For
current
information
about
PromoCell and PromoKine distributors
see www.promocell.com/distributor or
contact us via e-mail or phone.

Technical Support
Providing excellent technical support
for our products is our top priority. Our
technical service team relies on years of
laboratory experience to assist you with
product selection and advice, to maximize product performance.
You may reach our technical support via
telephone/fax (see numbers on the reverse side of the catalog) or by sending
an e-mail to: tech.support@promocell.com

Technical support is completed by our


online Knowledge Base:
www.promocell.com/knowledge-base
www.promokine.info/knowledge-base
It provides reference literature, application notes, and a library of technical
questions and answers related to our
products.

Product Use
Products are sold for research use only
and are not intended for use in diagnostic procedures or for therapeutic and
medical purposes.

Trademarks Used in this Catalog


The trademarks, logos, and service marks
(collectively the Trademarks) displayed
in this catalog, including but not limited
to those contained in the list below are
trademarks or registered trademarks of
PromoCell GmbH in Europe, the U.S.
and other countries. All other brands
and product names are trademarks or
registered trademarks of their respective
companies. Nothing contained in this
catalog should be construed as granting,
by implication, estoppel, or otherwise,
any license or right to use any trademarks

displayed in the catalog without the


permission of PromoCell GmbH or such
third party that may own the trademarks
displayed in the catalog. The misuse of
the trademarks displayed in the catalog,
or any other content in the catalog, is
strictly prohibited. Please be advised that
PromoCell GmbH will aggressively enforce its intellectual property rights to
the fullest extent of the law, including
the seeking of criminal prosecution.
PromoCell (PromoCell GmbH)
PromoFectin (PromoCell GmbH)
PromoFluor (PromoCell GmbH)
PromoKine (PromoCell GmbH)
DetachKit (PromoCell GmbH)
SupplementPack (PromoCell GmbH)
SupplementMix (PromoCell GmbH)
Cell Culture Assistant (PromoCell GmbH)
ABI Prism
Alexa Fluor
Cell Lab Quanta SC
Cepheid Smart Cycler
CyTM
LightCycler
Mastercycler
MitoCaptureTM
Rhodamine Green
RNAlater
Texas Red

Appendix

To place an order please use any of the


following convenient options:

206

Notes

Appendix

Notes

Notes

207

Appendix

Notes

208

Notes

Appendix

Notes

Fax Order Form


Shipping Address
Mr.

The Cell Culture Experts


www.promocell.com

Invoice Address (if different from shipping address)


Ms.

Dr.

Prof.

Mr.

Ms.

First Name

First Name

Last Name

Last Name

Company / University

Company / University

Department / Institute

Department / Institute

Street, No

Street, No

ZIP, City

ZIP, City

Country

Country

Dr.

Prof.

Phone
E-mail

Order Details
Catalog Number

Product Name

Quantity

Your Cell Research Partner


www.promokine.info

Please write in capital letters

Training Courses
www.promocell-academy.com
Please visit our websites regularly for upcoming new products and training courses

Please send me a copy of the

Please send me your newsletters

PromoCell catalog

PromoCell

PromoKine catalog

PromoKine

PromoCell Academy catalog

PromoCell Academy

Date, Signature

PromoCell GmbH
Sickingenstr. 63/65
69126 Heidelberg
Germany
USA/Canada
Phone:
1 866 251 2860 (toll free)
Fax:
1 866 827 9219 (toll free)
Deutschland
Telefon: 0800 776 66 23 (gebhrenfrei)
Fax:
0800 100 83 06 (gebhrenfrei)
France
Tlphone: 0800 90 93 32 (ligne verte)
Tlfax:
0800 90 27 36 (ligne verte)

2013 | 2014

Cell Culture & Cell Biology

2013 | 2014

Cell Culture & Cell Biology

United Kingdom
Phone:
0800 96 03 33 (toll free)
Fax:
0800 169 85 54 (toll free)
Switzerland
Phone:
+49 6221 649 34 0
Fax:
+49 6221 649 34 40
Netherlands
Phone:
+49 6221 649 34 0
Fax:
+49 6221 649 34 40
Other Countries
Phone:
+49 6221 649 34 0
Fax:
+49 6221 649 34 40

Email: info@promocell.com
info@promokine.info

www.promocell.com

Human Primary Cells & Media


Stem and Blood Cells & Media
Cell Biology Products