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Mohammed 1

I observed and tested different functions of a compound light microscope. Each part of a
microscope carries out a different purpose. I observed how each objective lens has its
own individual focus on a specimen, and depending on the contrast set the details one can
make out of a specimen.
Microscopes allow humans to observe what the naked eye cannot. There are various
microscopes depending on how much one wishes to magnify. Compound light
microscopes, for example, can help one magnify slides of dead organisms, like parts of a
flower or living microorganisms, such as bacteria. Each component of the compound
microscope enables its user to observe the specimen in the best way possible. The oculars
and objective lenses scan and magnify the object, and the focusing knobs allow one to
decide how much they would like to focus the image.

Compound light microscope
Prepared slides: Letter e slide, three threads slide (in yellow, red, and purple),
diatoms, and golgi apparatus.
Glass microscope slide
Glass cover slip
Pond water

 Observe the prepared slides using the compound light microscope.
 Take the glass microscope slide and place a drop of pond water on it.
 Cover it with a cover slip making sure there are no air bubbles.
 Observe the wet mount under the microscope.
3.2 Characteristics of a microscope

Located at the arm

Light source

Maximum revolving
Made up of stage clips
2 knobs, fine adjustment
and coarse adjustment

Focusing knobs

Identification of
Varies the intensity of light
Focuses the light onto the
Holds the slide in place
Fine adjustment focuses
low power, coarse focuses

4 H Units of Measurements 1 mm= 1.25 . The specimen moved to the left when I moved it to the right through the microscope. 4. The image was right side up.000001 mm Conclusion: Compound light microscopes have four power levels for the objective lens and depending on how small the specimen or what type different powers may be used.000.25 0. When observing a specimen one sees the opposite. it moves to the left.3 E orientation of the image compared to the specimen 2. Condenser lens: focus the light on the specimen b.Mohammed 2 Objectives Second set of lenses Oculars Set of lenses 3. The specimen moved up when I moved it away from me. Iris diaphragm: opens or closes the condenser .3 Objectives present on my compound microscope Objective Low-power Medium-power High-dry Oil-immersion Objective magnifying power 10x 40x 100x 45x Scans the specimen Magnifies the specimen Total magnifying power 100 400 10000 4500 Numerical Aperture 0. What is the function of the following parts of a microscope? a. Discussion: 1. Wet mounts can be used to observe live organisms like. bacteria in pond water.1 1.66 3. and it was not backwards or upside down. 3. 000 nm 1 nm= . If you move it to the right. 3.4 Order of Threads Location Closest to slide Middle Closest to cover slip Color Yellow Red Purple 3. The light microscope measures in micrometers and electric microscopes measure in nanometers.

and place a cover slip over it. 8. Ocular: magnifies 2. 3. Why were humans unaware of microorganisms for most of their history? Because microorganisms could not be seen without a microscope. 5. Describe the importance of the following concepts to microscopy: a. None of the microscopes moving parts should be forced. through the ocular lens and into the eye. Resolving power: helps one tell apart two images or details. through the objective lens. 6. What happens to contrast and resolving power when the aperture of the condenser of a compound light microscope is decreased? When the condenser is decreased contrast increases and resolving power decreases. In order list the lenses in the light path between a specimen viewed with the compound light microscope and its image on the retina of the eye.Mohammed 3 c. add a drop of water. and any loose dust should be blown off. . Describe how to make a wet mount Place the specimen on a microscope glass slide. Objective: scans the specimen d. b. B. A camera mounted on an electric microscope took this photo. 7. c. What happens to the field of view in a microscope when the total magnification is increased? It gets smaller. Magnification: helps enlarge the specimen so it can be observed better. 4. Describe how you would care and put away your microscope The microscope must be grasped from the arm when taking it out. wrap its cord around the oculars. When putting the microscope away remove the last slide. and place the dust cover back on. through the scope. The light passes through or from the specimen. 10. Contrast: illuminates the details of a specimen. or putting it back. 9. The dust cover must be removed before using.