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BIO3V20 General Microbiology (5 credits) Spring 2006 Schedule (version 1.0) TR 2:00-3:15 pm FO3.616 (lecture) TR 3:30-6:00 pm MP2.202 (lab) Dr.

Ernie Hannig Office: FO 3.714 Phone: 972-883-2505 email hannig@utdallas.edu Office hours: by appointment TAs: Swati Ghatpandi Sirisha Kodeboyina Jennifer Morris

Required Textbooks: Madigan M.T., Martinko, J.M., and Parker, J. (2003) Brock Biology of Microorganisms, 10th ed. Prentice Hall/Pearson Education, Inc., Upper Saddle River, NJ. [ISBN 0-13-066271-2] (lecture) Note: (2006) 11 th edition [ISBN 0-13-144329-1] is equally suitable. Cappuccino. J.G., and Sherman, N. (2005) Microbiology: A Laboratory Manual, 7th ed. Benjamin Cummings [ISBN 0-80532836-X] (lab) Recommended (atlas) Alexander, S.K., and Strete, D. (2001) Microbiology: A Photographic Atlas for the Laboratory. Benjamin Cummings [ISBN 08053-2732-0] Recommended (highly) Reading De Kruif, P. Microbe Hunters. This book was originally published in 1926, but reprint editions are available. Date Lecture Chapter (Brock, 10 th edition) Note: 11 th
edition is essentially identical for these chapters

Lab

Chapter
(Cappuccino and Sherman, 7th edition) [6th edition chapters in brackets if different]

Jan. 10 (Tues) Jan. 12 (Thurs.)

Jan. 17 (Tues.)

Welcome Microorganisms and Microbiology An Overview of Microbial life Macromolecules Cell Structure/Function Cell Structure/Function

Ch. 1 (21 pgs) Ch. 2 (17 pgs) Ch. 3 (17 pgs) (this should be a review) Ch. 4 (47 pgs) Ch. 4 (47 pgs)

Seminar 4 pm. Dr. Gireesh Rajashekara "Identification of Brucella melitensis virulence genes"

Jan. 19 (Thurs.)

Seminar 4 pm. Dr. Scott Landfear. "An Essential Role for Glucose Transporters in the Life Cycle of Leishmania Parasites"

Jan. 24 (Tues.) Jan. 26(Thurs.)

Cell Structure/Function Nutrition, Culture, and Metabolism I Nutrition, Culture, and Metabolism II

Ch. 4 (47 pgs) Ch. 5 (35 pgs) + handouts Ch. 5 (35 pgs) + handouts Introduction Lab Safety Microscopy: Measurements of microorganisms Bacterial staining: preparation, simple staining Continue and finish Exp. 4; 6, 8, 9 pgs. xii-xvi;

Jan. 31(Tues.)

Exp. 4; 6, 8, 9

Feb. 2 (Thurs.)

Nutrition, Culture, and Metabolism III

Ch. 5 (35 pgs) + handouts

Feb.7 (Tues.) Feb. 9 (Thurs.) Feb. 14 (Tues.)

Microbial Growth I
Microbial Growth II

Ch. 6 (30 pgs) Ch. 6 (30 pgs) Ch. 1-6

Negative stain; Gram stain Acid-fast stain Cultivation and cultural characteristics of microorganisms Finish Exp. 1-3 Bacterial growth: physical factors (oxygen) Serial dilution: viable cells Finish Exp. 18-20

Exp. 10, 11 Exp.12 Exp. 1-3

Exam I

Feb. 16 (Thurs.) Feb. 21 (Tues.)

Principles of Microbial Molecular Biology I Principles of Microbial Molecular Biology II Regulation of Gene Expression Bacterial Genetics I

Ch. 7 (40 pgs) Ch. 7 (40 pgs)

Exp. 18-20 [17-19]

Feb. 23 (Thurs.)

Ch. 8 (25 pgs)

Feb. 28 (Tues.)

Ch. 10

Mar. 2 (Thurs.) Mar. 7 (Tues.) Mar. 9 (Thurs.) Mar. 14 (Tues.)

Bacterial Genetics II Spring Break Spring Break Microbial Evolution and Systemics

Ch. 10

Differential and selective media; Biochemical tests (extracellular enzyme activities for starch, lipid, and protein hydrolysis); IMViC tests (MR/VP) Finish Exp. 15, 22, 25B, 25C

Exp. 15, 22, 25B, 25C [14, 21, 24B, 24C]

Ch. 11 (30 pgs)

Mar. 16 (Thurs.)

Essentials of Virology I

Ch. 9 (33 pgs)

Biochemical tests (carbohydrate utilization; indole production; citrate and amino acid utilization Biochemical tests (litmus milk, catalase, oxidase) Biochemical tests (H2S, urease, nitrate reduction); start mixed unknowns Finish Exp. 26, 27, 29; continue exp. 33 (weekly until
completed)

Exp. 23, 24, 25A, 25D, 32 [22, 23, 24A, 24D, 31] Exp.28, 30, 31 [27, 29, 30] Exp. 26, 27, 29; start 33 (mixed unknowns) [25, 26, 28, 32]

Mar. 21 (Tues.)

Exam II

Ch. 7, 8, 10, 11 (note Ch. 9 is not included) Ch. 9 (33 pgs)

Mar. 23 (Thurs.)

Essentials of Virology II

Mar. 28 (Tues.) Mar. 30 (Thurs.)

Bacterial, Plant, and Animal Viruses I Bacterial, Plant, and Animal Viruses II Microbial Growth Control I

Chapter 16 (27 pgs) Chapter 16 (27 pgs)

Bacterial viruses: isolation, cultivation, and enumeration Parasitic protozoa

Exp. 39 [38] Exp. 35 [34] 41, 44, 46 [40, 43, 45]

Apr. 4 (Tues.)

Ch. 20 (32 pgs)

Apr. 6 (Thurs.)

Microbial Growth Control II Human-Microbe Interactions I Human-Microbe Interactions II Immunology Catch-up

Ch. 20 (32 pgs)

Growth control: physical agents (moist heat); Growth control: chemical agents (antibiotics and other chemical agents) Finish Exp. 41, 44, 46

Apr. 11 (Tues.)

Ch. 21 (28 pgs)

Mycology

Exp. 36 [35]

Apr. 13 (Thurs.) Apr. 18 (Tues.) Apr. 20 (Thurs.)

Ch. 21 (28 pgs) Ch. 22 (32 pgs)

Finish Exp. 36 Work on unknowns Work on unknowns

Apr. 25 (Tues.) Final Exam Spring Break: March 6-11 Last day of class: April 24 Exam week: April 25-May 1

Ch. 9, 16, 20, 21, 22