You are on page 1of 334

1 - Introduction

2 Troubleshooting
3 - Parts Lists
4 - Removal & Replacement
5 - Verification Procedures

CELL-DYN 3200 SYSTEM


SERVICE MANUAL
Part Number 9140300

4#0#,QWURGXFWLRQ Proprietary Information

4#0#,QWURGXFWLRQ
Table of Contents
1.1 Proprietary Information . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.2 Revision Log. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
1.3 About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Service Manual Section Descriptions . . . . . . . . . . . . . 3
Section 1: Introduction & General Data . . . . . . . . . . . . 3
Section 2: Troubleshooting . . . . . . . . . . . . . . . . . . . . . . 3
Section 3: Parts Lists . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Section 4: Removal & Replacement . . . . . . . . . . . . . . . 3
Section 5: Verification Procedures . . . . . . . . . . . . . . . . 3
Section 6: Preventive Maintenance & Total Call. . . . . . 3
1.4 CELL-DYN 3200 Operators Manual . . . . . . . . . . . . . . 3
1.5 Warranty Information. . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.6 System Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
The CELL-DYN 3200 Hematology System . . . . . . . . . . 4
System External Components . . . . . . . . . . . . . . . . . . . 4
System Internal Components . . . . . . . . . . . . . . . . . . . . 5
1.7 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Signal Words and Graphic Warning Conventions . . . 5
Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Graphic Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Biosafety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Laser Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.8 CELL-DYN 3200 Hematology Parameters . . . . . . . . . . 9
1.9 System Specifications . . . . . . . . . . . . . . . . . . . . . . . . . 10
CELL-DYN 3200 SYSTEM SERVICE MANUAL

1.1 Proprietary Information


This CELL- DYN 3200 System Service Manual was developed
solely for use with Abbott Laboratories equipment as specified
in the operating instructions. The Manual is copyrighted by
Abbott Laboratories. No part of this document may be
reproduced, stored, or transmitted in any form or by any means
electronic, mechanical, photocopied, recorded, or otherwise
without the prior written permission of Abbott Laboratories.
This CELL- DYN 3200 System Service Manual is published by
Abbott Diagnostics, a Division of Abbott Laboratories, Abbott
Park, IL 60064-3500, U. S. A. Please direct all inquiries
concerning information in this manual to the foregoing address.
CELL- DYN is a registered trademark of Abbott Laboratories.
Vacutainer is a registered trademark of Becton-Dickinson and
Company.
This service manual was developed to be used by trained
Abbott Laboratories Field Service Personnel. The revision
status of this manual is the responsibility of the manual holder.
In no event shall Abbott Laboratories or its subsidiaries be
liable for any damages incurred in connection with or arising
from the use of this manual by persons not fully trained by
Abbott Laboratories.

1998 by Abbott Laboratories. All rights reserved.

Part Number 9140300 August 1998

1-1

4#0#,QWURGXFWLRQ 1.2 Revision Log

1.2 Revision Log


The Revision Status of this manual is indicated below. Be sure
that the manual contains the latest revision number of all
pages.
Table 1-1

Revision Log

Revision

Date

Pages Revised/Added

TSBs Incorporated

ISAs Incorporated

August

Complete Revision

109 - 001A, 003

109 - 003, 004, 005, 006

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

1-2

4#0#,QWURGXFWLRQ 1.3 About This Manual

1.3 About This Manual

Section 4: Removal & Replacement

Introduction

This section contains the procedures to be used by the FSR for


the removal and replacement of subassemblies and
components. The procedures are presented in a paragraph
format and are accompanied by illustrations for added clarity.

This Service Manual contains the information necessary to


align, troubleshoot, repair, and maintain the CELL-DYN 3200
Automated Hematology System.

Service Manual Section Descriptions


The manual is composed of five sections. The following are
descriptions of the contents of each of the sections.

Section 1: Introduction
This section contains an introduction to the system, information
on the use of the manual, warranty information, safety
considerations, hematology parameters, and system
specifications.

Section 5: Verification Procedures


This section contains procedures that enable the FSR to verify
the integrity of various instrument functions. These procedures
may be used to facilitate troubleshooting, or be dictated by
Troubleshooting and Removal and Replacement.
This section also contains procedures such as adjustments,
alignments, and calibrations used to ensure proper instrument
performance. These procedures may be performed on a routine
maintenance basis, or be dictated by Removal and
Replacement.

1.4 CELL-DYN 3200 Operators Manual


Section 2: Troubleshooting
This section contains procedures to be used by the FSR in
identifying and isolating problems. The procedures are
presented in a flowchart format and are accompanied by
illustrations for added clarity. A detailed explanation of the use
of the flowcharts is given at the beginning of the section.

Operating instructions and routine maintenance procedures for


the CELL-DYN 3200 are included in the Operators Manual
provided with each system. It is essential that the service
engineer read and understand the Operators Manual, and be
able to correctly perform all routine operating functions before
attempting to troubleshoot and repair the system.

Section 3: Parts Lists


This section contains parts lists and graphics showing the
locations of FRUs. This section is used by the FSR in
conjunction with Troubleshooting and Removal & Replacement.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

1-3

4#0#,QWURGXFWLRQ 1.5 Warranty Information

1.5 Warranty Information

System External Components

Components comprising the CELL-DYN 3200 System have


been designed by Abbott Laboratories for optimal performance.
Substitution of reagents, accessories, or system components
may adversely affect performance and may invalidate any
warranty agreements.

The following is a list of the CELL-DYN 3200 external


components and conditions:

Operator Technique (Maintenance)

System Reagents

Isotonic Diluent
Diluent/Sheath
Hemoglobin Lyse
WBC Lyse
System Controls & Calibrators

CELL-DYN 3200 Whole Blood Control


OPTI-CAL
Latex Beads
Whole Blood Specimens

System Environmental Integrity (Temperature, RFI, ESD)

Power Line Integrity

Abbott Laboratories cannot accept responsibility for any clinical


results produced by use of reagents, calibrators, controls,
equipment, or supplies either not manufactured by or not
approved by Abbott Laboratories.

1.6 System Introduction


Each CELL-DYN 3200 System may be comprised of three or
four major hardware components: Analyzer, Video Display,
Keyboard, and optional Sample Loader.

The CELL-DYN 3200 Hematology System


When approaching a hematology system from a service
perspective, the word system must be emphasized because
there are both internal components (system hardware and
software) and external components (reagents, controls, etc.)
that comprise the complete system. The Service
Representative must realize that both of these types of
components can adversely affect overall system performance.
Therefore, it follows that all external components and
conditions such as reagents, environment, integrity of samples
and controls be checked and verified as correct before
performing service on the internal components. In the
investigation of any non-specific or data related complaint, the
system hardware and software should be the last components
of the system to be suspected as the cause of the problem.
CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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4#0#,QWURGXFWLRQ 1.7 Safety

System Internal Components


The following is a list of the CELL-DYN 3200 internal
components:

CELL-DYN 3200 System Components

Flow Panel
Optics Bench
Power Supplies
Measurement Electronics
Computer Electronics
A/D & D/A Electronics
Air Supply
Personal Computer Module
Tube Manipulation Hardware

Signal Words and Graphic Warning


Conventions
Signal Words
'$1*(5=##Denotes an immediate hazard which, if not
avoided, could result in serious injury or death. This signal word
represents the highest level of any hazardous situation.
:$51,1*=##Denotes a hazard which, if not avoided, could
result in moderate to serious injury.
&$87,21=##Denotes potential hazards that could result in
minor injury. Also, used for conditions or activities that could
threaten equipment performance.

Graphic Conventions

1.7 Safety
Operation, maintenance, and servicing of hematology systems
may expose individuals to potential safety and health hazards.
All work must be performed in accordance with procedures
described in the Abbott Operators and Service Manuals, or as
directed by an Abbott Technical Support Representative.
Warnings are inserted in this manual to alert personnel to
potential hazards. The standard warning conventions including
signal words (e.g. Caution) and symbols are described below.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

The general hazard icon identifies an activity or area


that may present a hazard to personnel or equipment.

The electrical hazard icon alerts personnel to the


possibility of electrical shock if procedural or
engineering controls are not observed.

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4#0#,QWURGXFWLRQ 1.7 Safety


The biohazard icon identifies an activity or area where
personnel may be exposed to infectious substances.

The laser hazard icon identifies an activity or area


where personnel will be exposed to an eye hazard if
procedural or engineering controls are not observed.

Clean-up spills of potentially infectious materials in accordance


with established biosafety practices. A generally accepted
procedure for cleaning such spills is to absorb the spill with
toweling or other absorbent material, wipe the area with a
detergent solution, and then wipe the area with an appropriate
tuberculocidal disinfectant such as 10% chlorine bleach
solution.
Prior to service or maintenance, the instrument should be
decontaminated in accordance with the following:
a. Remove and dispose of contaminated disposables in a
regulated medical waste container.

The electrostatic discharge icon identifies an activity or


area where personnel must wear a ground strap while
servicing the system.

Biosafety
:$51,1*=#327(17,$/#%,2+$=$5' Consider
all clinical specimens, reagent controls, surfaces, or
components that contain or have contacted human
blood or serum as potentially infectious. Wear gloves,
lab coats and safety glasses, and follow other
biosafety practices as specified in the OSHA
Bloodborne Pathogen Rule (29CFR Part 1910.1030)
or other equivalent biosafety procedures.

b. Clean and decontaminate the exterior of the


instrument using a detergent solution followed by a
10% chlorine bleach solution or other tuberculocidal
disinfectant. Flush the fluid pathway with chlorine
bleach solution as specified in the Operators Manual.
Dispose of waste in accordance with local, state and federal
regulations. Probes, needles, broken glass, slides, and other
sharps that are contaminated with potentially infectious
substances should be collected in a sharps container for
disposal as regulated medical waste. Contaminated gloves,
wipes, and other disposables should be placed in a standard
medical waste container.

:$51,1*=#327(17,$/#%,2+$=$5'#The
aspiration needle/probe is sharp and potentially
contaminated with infectious materials. Avoid contact
with the tip of the needle/probe.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

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4#0#,QWURGXFWLRQ 1.7 Safety

Electrical Safety
:$51,1*= (/(&75,&$/#+$=$5' When the
instrument is powered and protective covers are
removed, there are exposed electrical systems that
could startle or seriously injure personnel upon
contact. Use appropriate safety precautions to prevent
body and/or tool contact with live electrical
components, especially power supplies.

Laser Safety
DANGER: LASER LIGHT Avoid direct exposure to
beam. Do not look directly into the laser beam or any
reflection of the beam from a mirror-like surface.
When the access door, instrument top cover, or other
inner protective cover is removed, (Helium-Neon)
laser power up to 10 mW continuous wave at 632.8
nm in a beam with 1 mR divergence could be
accessible in the interior of the optics bench. This
amount of energy, with insignificant attenuation with
distance, is sufficient to cause eye damage.

The Class 1 Laser Product Label (Abbott PN 9230702A) is


shown below (Figure 1-1). The label consists of black lettering
against a white background. The label is located on the
backside of the instrument and positioned at a clearly visible
location.
The inner protective cover laser warning labels must not be
removed and are to remain legible. The protective housing
label (Abbott PN 9230701D) is shown in Figure 1-2. The label
consists of black lettering against a yellow background and
appears on the protective cover within the optics bench and on
the flow panel (see Figure 1-3 and Figure 1-4).

32109

Figure 1-1

Laser Product Label (PN 9230702A)

&$87,21: Use of controls or adjustments or


performance of procedures other than those specified
herein may result in hazardous laser light exposure. If
the instrument is used or modified in a manner not
specified by the manufacturer, the protection provided
by the instrument may be impaired.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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4#0#,QWURGXFWLRQ 1.7 Safety


.CUGT
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Figure 1-2

Laser Warning Label (PN9230702D)


Figure 1-3

Top Laser Warning Label Location

For beam alignment and other open beam configurations,


follow instructions provided by the Service Manual, Advisories,
and Bulletins regarding the requirement for using laser safety
eyewear. If required, assure laser eyewear are not damaged
and have an optical density of 1- 2 at a wavelength of 632.8
nm. During open beam configurations, assure that the beam is
confined to the laser bench area and only personnel with proper
eye protection are present.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

1-8

4#0#,QWURGXFWLRQ 1.8 CELL-DYN 3200 Hematology Parameters

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1.8 CELL-DYN 3200 Hematology


Parameters
The following tables list the CELL-DYN 3200 Hematology
Parameters and their abbreviations.
Table 1-2

Red Cell Parameters


Full Name

Red Blood Cell Concentration

RBC

Hemoglobin Concentration

HGB

Hematocrit

HCT

Mean Cell Volume

MCV

Mean Cell Hemoglobin

MCH

Mean Cell Hemoglobin Concentration


Red Cell Distribution Width

Table 1-3
Figure 1-4

Front Laser Warning Label Location

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Abbreviation

MCHC
RDW

White Cell Parameters


Full Name

Abbreviation

White Blood Cell Concentration

WBC

Neutrophil Concentration

NEU

Neutrophil Percentage

%N

Lymphocyte Concentration

LYM

Lymphocyte Percentage

%L

Monocyte Concentration

MONO

Monocyte Percentage

%M

Eosinophil Concentration

EOS

Part Number 9140300 August 1998

1-9

4#0#,QWURGXFWLRQ 1.9 System Specifications


Table 1-3

1.9 System Specifications

White Cell Parameters (Continued)


Full Name

Abbreviation

Eosinophil Percentage

%E

Basophil Concentration

BASO

Basophil Percentage

Table 1-4

Abbreviation

Platelet Concentration

PLT

Mean Platelet Volume

MPV

Platelet-crit

PCT*

Platelet Distribution Width

PDW*

* Clinical significance has not been established for these


parameters. Therefore they are not reportable in the US.
They are provided for laboratory use only.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Table 1-5

Physical Specifications (Unpackaged)

%B

Platelet Parameters
Full Name

The following tables contain the specification for the CELL-DYN


3200 System.

CS Model

SL Model

Graphics
Printer

Height

19.0 (48.2 cm)

19.0 (48.2 cm)

7.2 (18.3 cm)

Width

32.8 (83.3 cm)

32.8 (83.3 cm)

16.1 (40.9 cm)

Depth

23.3 (59.2 cm)

29.3 (74.4 cm)

9.9 (25.1 cm)

Weight

214 lb (97.3 kg) 232 lb (105.5 kg)

Table 1-6

9.9 lb (4.5 kg)

Physical Specifications (Packaged)


CS Model

SL Model

Graphics
Printer

Height

38 (96.5 cm)

38 (96.5 cm)

12 (30.5 cm)

Width

34 (86.3 cm)

34 (86.3 cm)

19.5 (49.5 cm)

Depth

31 (78.7 cm)

31 (78.7 cm)

14.8 (37.6 cm)

Weight

255 lb (116 kg)

273 lb (124 kg)

16.4 lb (7.4 kg)

Part Number 9140300 August 1998

1 - 10

4#0#,QWURGXFWLRQ 1.9 System Specifications


Table 1-7
Module

Analyzer

Analyzer &
Sample
Loader
Monitor
Printer

Analyzer Input Requirements

Table 1-8

Voltage

Frequency

Max
Current

110 VAC + 10 %

50/60 3 Hz

6 amps

120 VAC + 10 %

50/60 3 Hz

6 amps

220 VAC + 10 %

50/60 3 Hz

3 amps

240 VAC + 10 %

50/60 3 Hz

3 amps

110 VAC + 10 %

50/60 3 Hz

120 VAC + 10 %

Performance Specifications

Background Precision Correlation

Linear
Range

WOC

0.10K/L

2.7%

0.99

0.0-250 K/L

NOC

0.10 K/L

2.7%

0.99

0.0-250 K/L

RBC

0.20 M/L

1.5%

0.98

0.0-8.0 M/L

6 amps

HGB

0.1g/dl

1.0%

0.98

0.0-25.0g/dL

50/60 3 Hz

6 amps

MCV

N/A

1.0%

0.98

35-180 fL

220 VAC + 10 %

50/60 3 Hz

3 amps

50/60 3 Hz

RDW

N/A

5.0%

0.91

N/A

240 VAC + 10 %

3 amps

100 - 240 VAC

50/60

1.3 amps

PLT

5.0K/L

4.0%

0.98

0.0 - 1750
K/L

50/60

0.5 amps

MPV

N/A

5.0%

0.72

2.0-18.0 fL

120 VAC 10%


220 - 260 VAC

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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4#0#,QWURGXFWLRQ 1.9 System Specifications


Table 1-9

Operational Specifications

Operating Environment

Specimen Tube Dimensions


Diameter

13 mm
75 mm

Temperature

15o - 30o C (59o - 86o F)

Length

Relative Humidity

20% to 80%, RHNC

Minimum Specimen Volumes


Closed and Sample Loader Mode

Complete Cycle Times


Startup (From Power Off)
Startup (From Standby)
Count Cycle, Patient
Count Cycle + FrgWBC
Count Cycle + ResRBC
Shutdown (To Standby)
Shutdown (To Power Off)

~ 7 minutes
~ 5 minutes
~ 52 seconds
~ 100 seconds
~ 100 seconds
~ 130 seconds
~ 130 seconds

Open Mode

1 mL
500 L

Batch Size
1 to 50 tubes per batch
Bar Code Formats
Interleaved 2-of-5 code
Codabar
Code 39

Throughput

Code 128 (check digit only)

CS Model

Bar Code Specifications

Open

80 Samples/Hour

Printed on good quality label stock

Closed

68 Samples/Hour

0.25-inch quiet zone on each end

0.01-inch minimum narrow bar width

SL Model
Open

80 Samples/Hour

0.5-inch minimum bar length

Closed

68 Samples/Hour

2-inch maximum label length

1.25-inch maximum label width

Whole Blood Aspiration Volume


Open Mode

125 L

Closed Mode

250 L

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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5#7URXEOHVKRRWLQJ Table of Contents

TOC

5##7URXEOHVKRRWLQJ
Table of Contents
2.1
2.2
2.3

Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Troubleshooting Approach . . . . . . . . . . . . . 2-2
Error Messages . . . . . . . . . . . . . . . . . . . . . . . 2-3

IP-1
IP-2
IP-3
IP-4
IP-5
IP-6
IP-7
IP-8
IP-9
IP-10
IP-11
IP-12
IP-13
IP-14
IP-15
IP-16
IP-17
IP-18
IP-19
IP-20
IP-21
IP-22
IP-23
IP-24

Fluid Leaks, Open Mode Probe or Wash Block . . . 2-4


Fluid Leaks, Closed Mode Needle or Wash Block . 2-6
Incomplete Aspiration . . . . . . . . . . . . . . . . . . . . . . . 2-8
Flow Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-11
RBC/HGB Diluent Overpressure . . . . . . . . . . . . . 2-13
Optical Parameter Count Problems . . . . . . . . . . . 2-15
Reagent Empty . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-26
Optical Backgrounds Too High . . . . . . . . . . . . . . . 2-31
Histogram Problems . . . . . . . . . . . . . . . . . . . . . . . 2-32
Controls Out . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-33
Vacuum Accumulator #1 Wet . . . . . . . . . . . . . . . . 2-35
Vacuum Accumulator #2 Wet . . . . . . . . . . . . . . . . 2-37
HGB Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-39
X-B Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-41
Optical Parameters Imprecision . . . . . . . . . . . . . . 2-44
HGB Imprecision . . . . . . . . . . . . . . . . . . . . . . . . . 2-47
Primary Vacuum/Pressure Isolation, . . . . . . . . . . 2-48
Pressure Leak Isolation . . . . . . . . . . . . . . . . . . . . 2-57
Vacuum Leak Isolation . . . . . . . . . . . . . . . . . . . . . 2-64
Shear Valve Position Fault . . . . . . . . . . . . . . . . . . 2-70
Flags . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-72
External Waste Full . . . . . . . . . . . . . . . . . . . . . . . 2-74
Blank Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-76
HSSL Failures/Message Reception Time-Out . . . 2-77

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Hard Drive Errors . . . . . . . . . . . . . . . . . . . . . . . . . 2-78


Printer Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-79
Sample Loader & Aspiration Tower
Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . 2-81
Appendix 2A -Cable Connection Diagram . . . . . . . . . . . . 2A-1
Appendix 2B -System Block Diagrams . . . . . . . . . . . . . . 2B-1
Appendix 2C -System Flow Diagrams . . . . . . . . . . . . . . . 2C-1
IP-25
IP-26
IP-27

Part Number 9140300-August 1998

2-1

5#0#7URXEOHVKRRWLQJ 2.1 Summary

2.1 Summary

c. To refer to the appropriate IP or VP, click on the red


link.

This chapter contains the following information for CELL-DYN


3200:

d. Begin at the START box and follow the directions in the


IP.

Error Messages

Isolation Procedures (IPs)

Block Diagrams

Normal Scatter/Histograms

Service Commands

e. If a part is adjusted or replaced, go to Section 5.2,


Verification Matrix.
f.

Find the part in left column.

g. Perform VPs in order given (i.e., 1, 2, 3, 4 etc.)


2. Perform Total Call.

2.2 Troubleshooting Approach


Troubleshooting is required whenever an Observed Error or a
Displayed Error occurs. Displayed Errors can be viewed on the
Bulletin Line or status box of the Data Station screen. (The
Bulletin Line is down about 3/4 way on the screen.) Detailed
information can be found in the fault report screen of the
diagnostics submenu.
When a malfunction occurs, follow these recommended
procedures:
1. Use this Troubleshooting Guide as follows:
a. If possible, begin by verifying the reported error.
Perform the exact procedure that produced the error
condition.
b. When an error occurs, either observed or displayed,
look up the error in:

the Error Messages table or


IP 27, Sample Loader & Aspiration Tower Error
Messages table

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 - August 1998

2-2

5#0#7URXEOHVKRRWLQJ 2.3 Error Messages

2.3 Error Messages


Error

Error
Corrective Action

Corrective Action

Invalid Tube Height

IP-27

Leaks, Pressure

IP-18

Leaks, Vacuum

IP-19

Background, HGB Too High

IP-13

Background, RBC/ PLT Too High

IP-8

Background, WBC Too High

IP-8

Load Zone Empty Detector


Malfunction

IP-27

Bar Code Reader Failure

IP-27

MCH Errors

IP-14

C:\ Hard Drive Errors

IP-25

MCHC Errors

IP-14

Controls Out

IP-10

MCV Errors

IP-14

Diluent/Sheath Empty

IP-7

Message Reception Timeout

IP-24

Erratic Results, HGB

IP-13

Mix Head Not At Top Position

IP-27

Mix Head Stuck At Top Position

IP-27

Mix Head Stuck In Angle Position

IP-27

Erratic Results, Optical

IP-6 Ensure Blood Sam


ple is Fresh. Differential
count is not reliable
before 30 minutes or after
12 hours after sample is
drawn.

Mix Head Stuck In Vertical


Position

IP-27

Mix Zone Rack Position Error

IP-27

External Waste Full

IP-22

No Tube Present

IP-27

Flags

IP-21

Overpressure, Diluent Syringe

IP-5

Flow Errors

IP-4

Precision Problems

HGB IP-16, OPT IP-15

Fluid Leaks, Closed Mode Probe

IP-2

Pressure Errors

IP-17

Fluid Leaks, Open Mode Probe

IP-1

Printer Errors

IP-26

HCT Errors

IP-14

Probe Stuck At Home Position

IP-27

HGB Errors

IP-13

IP-27

Histogram Errors

IP-9

Probe Unable To Reach Home


Position

HSSL Com Failures

IP-24

Rack Bar Code Read Failure

IP-27

Incomplete Aspiration

IP-3

Shear Valve Position Fault

IP-20

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 - August 1998

2-3

5#0#7URXEOHVKRRWLQJ 2.3 Error Messages


Error

Corrective Action

Tube Dropped During Mixing

IP-27

Tube Lost Moving From Position


3 To 4

IP-27

Tube Position Bar Code Read


Failure

IP-27

Tube Stuck In Position 3

IP-27

Tube Stuck In Position 4

IP-27

Unexpected Tube In Position 4


After Rack Advance

IP-27

Unload Area Hardware


Malfunction

IP-27

Unload Zone Full Detector


Malfunction

IP-27

Unload Zone Nearly Full Detector


Malfunction

IP-27

Vacuum Accumulator 1 Wet

IP-11

Vacuum Accumulator 2 Wet

IP-12

Vacuum Errors

IP-17

WBC Imprecision

IP-15 Ensure Blood Sample is Fresh. Differential


count is not reliable
before 30 minutes or after
12 hours after sample is
drawn.

WBC/HGB Lyse Empty

IP-7

X-B Errors

IP-14

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 - August 1998

2 - 3A

5#7URXEOHVKRRWLQJ IP-1 Fluid Leaks, Open Mode Probe or Wash Block


IP-1 Fluid Leaks, Open Mode Probe or Wash Block (Page 1 of 2)
Start

YES

- Clamp tubing at the top of


Valve 11

- Clamp tubing between


Ultrasonic sensor and
Shear valve
NO

Probe Leaking
In Ready Mode

Leaking
Stops

Leaking
Stops

YES

NO

YES

Wash Block Leaking


In Ready Mode

- Verify connections on
Ultrasonic Sensor
- Verify and tighten fittings
on Y-Valve
- Verify connection on top
of Probe
- Replace Y-Valve (PL-8)

NO

YES
1

CELL-DYN 3200 SYSTEM SERVICE MANUAL

- Verify pinch tubing in


Valve 11
- Verify and replace
Valve 11 (PL-4.1)

NO

- Verify Shear valve


fittings
- Clean Shear Valve
(VP-48)
- Replace Shear Valve
(PL-9.1)

Part Number 9140300-August 1998

2-4

5#7URXEOHVKRRWLQJ IP-1 Fluid Leaks, Open Mode Probe or Wash Block


IP-1 Fluid Leaks, Open Mode Probe or Wash Block (Page 2 of 2)

- Verify pinch tubing in


Valve 15
- Verify and replace Valve
15 (PL-4.1)

- Run a several Count


Cycles while observing the
bottom of the Wash Block

YES

- Ensure fittings on Wash


block are not leaking

NO

Liquid
Leaking
?

CELL-DYN 3200 SYSTEM SERVICE MANUAL

- Ensure Vacuum #1 and Pressure #3


are within specifications (VP-16)
- Ensure bottom fitting on Wash Block
is not restricted
- Ensure tubing between Wash Block
and Valve 14 is not restricted
- Verify operation of Valve 14 and
replace if necessary (VP-10), (PL-4.1)
- Replace pinch tubing in Valve 14
(TM-1)
- Ensure tubing between Valve 14 and
WC #4 is not restricted
- Verify operation of right Vent Trap
Check Valve and replace if necessary
(TM-1)
- Ensure tubng between WC #4 and
Valve 31 is not restricted
- Verify operation of Valve 31 and
replace if necessary (VP-10), (PL-4.1)
- Replace pinch tubing in Valve 31
(TM-1)

Part Number 9140300 August 1998

2-5

5#7URXEOHVKRRWLQJ IP-2 Fluid Leaks, Closed Mode Needle or Wash Block


IP-2 Fluid Leaks, Closed Mode Needle or Wash Block (Page 1 of 2)

Start

- Clamp tubing at the top of


Valve 11

- Clamp tubing between


Ultrasonic sensor and
Shear valve

Leave tubing connected, and


remove Needle from Wash Block

NO

YES

Leaking
Stops
Probe Leaking
In Ready Mode
?

Leaking
Stops

YES

NO
YES

Wash Block Leaking


In Ready Mode

- Verify connections on
Ultrasonic Sensor
- Verify and tighten fittings
on Y-Valve
- Verify connection on top
of Probe
- Replace Y-Valve (PL-8)

NO

YES
1
CELL-DYN 3200 SYSTEM SERVICE MANUAL

- Verify pinch tubing in


Valve 11
- Verify and replace
Valve 11 (PL-4.1)

NO

- Verify Shear valve


fittings
- Clean Shear Valve
(VP-48)
- Replace Shear Valve
(PL-9.1)

2
Part Number 9140300 August 1998

2-6

5#7URXEOHVKRRWLQJ IP-2 Fluid Leaks, Closed Mode Needle or Wash Block


IP-2 Fluid Leaks, Closed Mode Needle or Wash Block (Page 2 of 2)

- Verify pinch tubing in


Valve 34
- Verify and replace Valve
34 (PL-4.1)

- Run a several Count


Cycles while observing the
bottom of the Wash Block

YES

- Ensure fittings on Wash


block are not leaking

NO

Liquid
Leaking
?

CELL-DYN 3200 SYSTEM SERVICE MANUAL

- Ensure Vacuum #1 and Pressure #3 are


within specifications (VP-16)
- Ensure bottom fitting on Wash Block is not
restricted
- Ensure tubing between Wash Block and
Valve 37 is not restricted
- Verify operation of Valve 37 and replace if
necessary (VP-10), (PL-4.1)
- Replace pinch tubing in Valve 37 (TM-1)
- Ensure tubing between Valve 37 and WC #4
is not restricted
- Verify operation of right Vent Trap Check
Valve and replace if necessary (TM-1)
- Ensure tubng between WC #4 and Valve 31 is
not restricted
- Verify operation of Valve 31 and replace if
necessary (VP-10), (PL-4.1)
- Replace pinch tubing in Valve 31 (TM-1)

Part Number 9140300 August 1998

2-7

5#7URXEOHVKRRWLQJ IP-3 Incomplete Aspiration


IP-3 Incomplete Aspiration (Page 1 of 3)

Start

NO

- Verify Y-Valve is rotating


properly (VP-7) .

- Aspirate Normal
Control in both open
and closed modes.

YES

- Clean Shear Valve (VP-48) .

Rotating
Properly
Problem
Corrected
?
YES

Mode(s)
Affected

?
Open Only

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Both

NO
Closed Only
- Replace Y-Valve Assy (PL-8) .
- Replace MDM (Pl-9.2)

Total Call

- Replace SDM/CDM (PL-15) .

Part Number 9140300 August 1998

2-8

5#7URXEOHVKRRWLQJ IP-3 Incomplete Aspiration


IP-3 Incomplete Aspiration (Page 2 of 3)

- Run an open cycle and


time the blood movement
to the Ultrasonic Sensor.
Start when Valve 12
opens and stop when
blood reaches sensor.
YES

< 3.5 Seconds


?

- Run a closed cycle and


time the blood
movement to the
Ultrasonic Sensor. Start
when Valve 12 opens
and stop when blood
reaches sensor.

- Place a blood sample


under Open Probe and
manually open Valve 12
until the blood is just
past the Y-Valve.
- Verify an air gap of
approximately 1 inch
between the diluent and
blood columns.

- Manually place a blood


sample under Closed
Probe.
- From Main Menu, press
DIAGNOSTICS
YES

MORE
TOWER TEST
NEEDLE DOWN

- If no air gap is present,


check the Open Probe
plumbing for air leaks.

- Manually open Valve 12


until the blood is just
past the Y-Valve.

< 7.0 Seconds

- Verify an air gap of


approximately 3 inches
between the diluent and
blood columns.

?
NO

NO
- Clean Closed Probe (VP-51).
- Clean Open Probe (VP-50).
- Ensure there are no
restrictions between Open
Probe and Y-Valve.
- Replace Open Probe (PL-8).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

- Ensure there are no


restrictions between Closed
Probe and Y-Valve.

NO

- Replace Closed Probe (PL-8).


- Verify Valve 38 (VP-10) and
ensure Vent Trap plumbing is
free of restrictions.

Air Gap
Present
?

- Check the Closed


Probe plumbing for air
leaks.

Part Number 9140300 August 1998

YES
3

2-9

5#7URXEOHVKRRWLQJ IP-3 Incomplete Aspiration


IP-3 Incomplete Aspiration (Page 3 of 3)

- INITIALIZE and PRIME


instrument.
- Run a closed cycle and
verify that the blood
column stops just past
Sample Sensor #2.

- Run an open cycle and


time the blood
movement to the
Ultrasonic Sensor. Start
when Valve 12 opens
and stop when blood
reaches sensor.

- Run an open cycle and


verify that blood column
stops just past
Ultrasonic Sensor. I
blood does not stop:

- If blood does not stop:

- Re-seat Ultrasonic
Sensor Cable.

- Perform Sample Sensor


adjustment (VP-12).

- Replace Ultrasonic
Sensor (PL-8).

- Re-seat sensor cable.

- Replace Ultrasonic
Sensor Board (PL-9.2).

- Replace Sample
Sensor (PL-9.1).
- Replace Sample
Sensor Board (PL-9.2).

YES

< 3.5 Seconds


?

NO

- Verify Vac 2 level (VP-16). Troubleshoot as necessary.


- Ensure there are no restrictions between Y-Valve and
Valve 12
- Verify Valve 12 and tubing (VP-10). Replace as necessary
(PL-4.1), (TM-1).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 10

5#7URXEOHVKRRWLQJ IP-4 Flow Errors


IP-4 Flow Errors (Page 1 of 2)
Sample
Staging
Pump

Start
From Main Menu:
YES
- Run a Count Cycle and observe
the Delivery Syringe and WC1.
Verify that when the syringe is
moving up, liquid is dripping into
WC1 at a fast and constant rate
(See Figures).

DIAGNOSTICS
MORE
MOTOR OPERATION
EXERCISE MOTORS

WC1

- Type A and press Enter.


- Observe the Delivery Syringe
and verify smooth upward
movement (See Figures).

68

Delivery Syringe

Dripping
OK
?

NO

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Smooth
Movement
?

YES

NO

Part Number 9140300 August 1998

2 - 11

5#7URXEOHVKRRWLQJ IP-4 Flow Errors


IP-4 Flow Errors (Page 2 of 2)

- Verify level of 9 psi (VP-16)

- Check Laser for stable


output (VP-46).

- Verify tubing and operation of


Valves 64, 65, 91, and 56 and
replace as necessary (TM-1),
(PL-4.1).

- Remove and clean Delivery


Syringe.
- Replace Delivery Syringe
(PL-7).
- Replace Syringe Driver
Assembly (PL-7).
- Replace MPM (PL-11).

- Perform Optical Flow Cell


Cleaning Procedure (VP-44).
- Replace In-Line Sheath Filter
(PL-4.2).

Output
Stable

YES

NO

- Remove, inspect, and clean


Flow Cell Nozzle.
- Replace Flow Cell Assy. (PL-10).

- Replace Laser (PL-10).


- Replace Laser Power Supply
(PL-2.2).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 12

5#7URXEOHVKRRWLQJ IP-5 RBC/HGB Diluent Overpressure


IP-5 RBC/HGB Diluent Overpressure (Page 1 of 2)

Start

- Check resistance of
Overpressure Sensor

- Remove, inspect, and clean


Shear Valve (VP-48)
NO

Sensor
Shorted
?

- Verify Shear Valve rotation time


is <1.1 sec
From Main Menu:
DIAGNOSTICS

- Ensure diluent paths from


syringe to RBC and HGB
Mixing Chambers are free of
restrictions
- Verify operation of Valves 27
and 26 (VP-10) and replace if
necessary (PL-4.1)

MORE
MOTOR OPERATION
SHEAR VAL DISPENSE
NO

SHEAR VAL TIME


SHEAR VAL ASPIRATE
SHEAR VAL TIME

YES
- Replace Sensor (PL-4.2)
Problem
Corrected

Times
OK
YES

?
Total Call

?
NO

YES

Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 13

5#7URXEOHVKRRWLQJ IP-5 RBC/HGB Diluent Overpressure


IP-5 RBC/HGB Diluent Overpressure (Page 2 of 2)

In Ready Mode, try to manually


rotate the front and back of the
Shear Valve.

Shear Valve Sections


Should Not Move

- Lubricate Shear Valve Driver


(VP-42)
If problem persists:
- Replace Shear Valve Driver
(PL-9.1)

If there is movement:
- Replace defective section:
Front (PL-9.1)
Rear (PL-9.1)
If problem persists:
- Replace Shear Valve Driver
(PL-9.1)

Rim Notch
Faces Right
Total Call
32002

Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 14

5#7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems


IP-6 Optical Parameter Count Problems (Page 1 of 11)

Note: This procedure assumes that HGB Results are OK in Open and Closed Modes, and there are no hardware or software Error Messages.
It is designed to find and correct gross WBC, NOC, RBC, and PLT problems. For Optical Precision Problems see IP-15.
Start

- Verify proper Reagents and Expiration


Date.
- Perform Background Count Verification
(VP-56).

Backgrounds
OK

NO

Go to (IP-8),
correct and
return here.

All "0"

YES

RBC Only "0"

NO

NO

RBC & PLT


Only "0"

YES

PLT Only "0"

YES

YES

?
YES

NO

NO

NO

From Main Menu, press


RUN
SPECIMEN TYPE
FRAGILE WBC
- Run a Normal Control five times and
record the optical values.

WBC Only "0"

YES

NOC Only "0"

YES

NO

- Compare the results to the Data Sheet.


1

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 15

5#7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems


IP-6 Optical Parameter Count Problems (Page 2 of 11)

NOC Only
Low
All Low

YES

YES

WBC Only
High

12

NO

NO

YES

15

NO
All High
RBC & PLT
Only Low

YES

YES

NOC Only
High

13

?
NO

16

10

YES

NO

NO

NO
RBC & PLT
Only High
WBC Only
Low

YES

11

YES

14

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 16

5#7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems


IP-6 Optical Parameter Count Problems (Page 3 of 11)

All Erratic

YES

WBC Only
Erratic

17

NO

NO

RBC & PLT


Only Erratic
?

YES

18

NO

NOC Only
Erratic

YES

YES

19

20

?
NO
Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 17

5#7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems


IP-6 Optical Parameter Count Problems (Page 4 of 11)

3
- Verify beam alignment
(VP-20), (VP-21).

- Verify laser power (VP-46).


- Verify samples are being staged to
Optical Flow Cell by Peri-pump.
Troubleshoot as necessary.

YES

- Verify Sheath Reservoir is filling


properly.
- Verify Pressure 2 (VP-16) and
proper Sheath flow to Optical Flow
Cell. Troubleshoot as necessary.
- Replace In-Line Filter (PL-4.2).
- Verify operation of Sample
Injection Syringe (VP-6), (VP-7).
Troubleshoot as necessary.
NO

Power OK

- If not able to get data on


the Extended Count
Screen, replace in order
the Optical Flow Cell,
MAM, SPM, and CPU/
D C M (PL-10), (PL-12),
(PL-13).

?
NO
NO
- Connect new Laser Head to
Laser Power Supply and
verify power (see Warning).
If good, replace Laser Head
(PL-10). If not, replace Laser
Power Supply (PL-2).

Problem
Corrected
?
YES

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'DQJHU
RII1#$#KLJK#YROWDJH#FKDUJH#PD\#UHPDLQ#RQ#WKH#ODVHU#FRQQHFWRU#
ZLWK#WKH#SRZHU#RII1#'R#QRW#WRXFK#WKH#PDOH#SLQV1#6KRUW#PDOH#SLQV#
WR#&KDVVLV1

Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 18

5#7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems


IP-6 Optical Parameter Count Problems (Page 5 of 11)

- Verify RBC/PLT Sample is being


staged to Optical Flow Cell.

- Verify WBC Sample is being


staged to Optical Flow Cell.

- Verify operation of Valve 54 (VP10) and tubing. Replace as


necessary (PL-4.1), (TM-1).

- Verify operation of Valve 53


and 55 (VP-10) and tubing.
Replace as necessary (PL4.1), (TM-1).

- From Main Menu, press


DIAGNOSTICS
F12
F1
SET POINT ENTRY
- Verify RBC/PLT Threshold is
550.
- Reseat Preamp, MAM, SPM,
CPU/DCM Cables.
- Replace MAM (PL-12).
- Replace 10 o Preamp (PL-10).
- Replace SPM (PL-10).
- Replace CPU/DCM (PL-13).

- Replace MAM (PL-12).

- Replace MAM (PL-12).

- Replace SPM (PL-10).

- Replace SPM (PL-10).

- Replace CPU/DCM (PL-13).

- Replace CPU/DCM (PL-13).

- Verify operation of WBC


Syringe (VP-6), (VP-7).
- Verify NOC Sample is
being staged to Optical
Flow Cell.

- From Main Menu, press


DIAGNOSTICS
F12

- Verify operation of Valve


41 (VP-10) and tubing.
Replace as necessary
(PL-4.1), (TM-1).

F1
SET POINT ENTRY
- Verify WBC Threshold is
360.

- From Main Menu, press

- Reseat Preamp, MAM, SPM,


CPU/DCM Cables.

DIAGNOSTICS

- Replace MAM (PL-12).

F1

F12

- Replace 0 Preamp (PL-10).

SET POINT ENTRY

- Replace SPM (PL-10).

- Verify WBC Threshold is


400.

- Replace CPU/DCM (PL-13).

- Perform NOC Gain


Adjustment (VP-25).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 19

5#7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems


IP-6 Optical Parameter Count Problems (Page 6 of 11)

9
- Verify laser power (VP-46).
- Clean Laser Bench (VP-43).
-- Verify Sheath Reservoir is filling
properly.

YES

- Verify Pressure 2 (VP-16) and


proper Sheath flow to Optical Flow
Cell. Troubleshoot as necessary.

Power OK

- Replace In-Line Filter (PL-4.2).


- Verify operation of Sample
Injection Syringe (VP-6), (VP-7).
Troubleshoot as necessary.
- Replace Vent Accumulator Filter
(PL-4.2).

NO

Problem
Corrected
?
YES

?
NO

- Clean, debubble, and wet


Optical Flow Cell (VP-44),
(VP-45).
- Verify beam alignment (VP20), (VP-21).
- Replace Optical Flow Cell (PL10).
- Replace MAM (PL-12).

NO

- Replace CPU/DCM (PL-13).

- Connect new Laser Head to


Laser Power Supply and
verify power (See Warning).
If good, replace Laser Head
(PL-10). If not, replace
Laser Power Supply (PL-2).

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'DQJHU
LV#RII1#$#KLJK#YROWDJH#FKDUJH#PD\#UHPDLQ#RQ#WKH#ODVHU#
FRQQHFWRU#ZLWK#WKH#SRZHU#RII1#'R#QRW#WRXFK#WKH#PDOH#SLQV1#
6KRUW#PDOH#SLQV#WR#&KDVVLV1

Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 20

5#7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems


IP-6 Optical Parameter Count Problems (Page 7 of 11)
11

10

- Verify RBC/PLT Sample is


being staged to Optical
Flow Cell.
- Verify operation of Valve 54
(VP-10) and tubing.
Replace as necessary (PL4.1), (TM-1).
- Clean Shear Valve (VP-48).

Problem
Corrected
?

- From Main Menu, press


DIAGNOSTICS
F12
F1
SET POINT ENTRY
N O - Verify RBC/PLT Threshold
is 550.

- From Main Menu, press


- Verify WBC Sample is
being staged to Optical
Flow Cell.
- Verify operation of Valve 53
and 55 (VP-10) and tubing.
Replace as necessary (PL4.1), (TM-1).

- Verify 0 o and 10 o Log


Amplifiers (VP-28).

- Verify operation of WBC


Syringe (VP-6), (VP-7).

- Verify 0 o and 10 o Gain (VP26), (VP-27).

- Clean Shear Valve (VP-48).

DIAGNOSTICS
F12
F1
SET POINT ENTRY
NO

- Verify WBC Threshold is


360.
- Verify WBC Gain (VP-23).
- Replace MAM (PL-12).
- Replace SPM (PL-10).
- Replace CPU/DCM (PL-13).

- Replace MAM (PL-12).


- Replace SPM (PL-10).
- Replace CPU/DCM (PL-13).

YES

Problem
Corrected
?

Total Call
YES
Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 21

5#7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems


IP-6 Optical Parameter Count Problems (Page 8 of 11)

12

- Verify NOC Sample is being


staged to Optical Flow Cell.
- Verify operation of Valve 41
(VP-10) and tubing. Replace as
necessary (PL-4.1), (TM-1).
- From Main Menu, press
DIAGNOSTICS
F12

13

14

- Clean Laser Bench (VP-43).

- From Main Menu, press

- Clean, debubble, and wet


Optical Flow Cell (VP-44),
(VP-45).

DIAGNOSTICS

- Verify beam alignment (VP20), (VP-21).

SET POINT ENTRY

- Replace Optical Flow Cell


(PL-10).

F1

- Replace MAM (PL-12).

SET POINT ENTRY

- Replace CPU/DCM (PL-13).

- Verify NOC Threshold is 400.


- Perform NOC Gain Adjustment
(VP-25).

F12
F1
- Verify RBC/PLT Threshold is
550.
- Verify 0 o and 10 o Log Amplifiers
(VP-28).
- Verify 0 o and 10 o Gain (VP-26),
(VP-27).
- Replace MAM (PL-12).
- Replace SPM (PL-10).
- Replace CPU/DCM (PL-13).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 22

5#7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems


IP-6 Optical Parameter Count Problems (Page 9 of 11)

15

16

- From Main Menu, press


- Verify operation of WBC
Syringe (VP-6), (VP-7).

DIAGNOSTICS
F12

- Clean Shear Valve (VP-48).

SET POINT ENTRY


- Verify WBC Threshold is
360.
- Verify WBC Gain (VP-23).

Problem
Corrected
?

DIAGNOSTICS
F12

F1
NO

- From Main Menu, press

- Replace MAM (PL-12).

F1
SET POINT ENTRY
- Verify NOC Threshold is
400.
- Perform NOC Gain
Adjustment (VP-25).

- Replace SPM (PL-10).


- Replace CPU/DCM (PL-13).

YES
Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 23

5#7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems


IP-6 Optical Parameter Count Problems (Page 10 of 11)

17

- Verify stable laser power


(VP-46).

- Verify samples are being staged to


Optical Flow Cell by Peri-pump.
Troubleshoot as necessary.

YES

- Verify Sheath Reservoir is filling


properly.
- Verify Pressure 2 (VP-16) and
proper Sheath flow to Optical Flow
Cell. Troubleshoot as necessary.

NO

Power Stable

- Replace MAM (PL-12).

- Replace SPM (PL-12).


- Replace CPU/DCM (PL-13).

- Replace Vent Accumulator Filter


(PL-4.2).

NO

- Verify operation of Sample


Injection Syringe (VP-6), (VP-7).
Troubleshoot as necessary.

- Connect new Laser Head to


Laser Power Supply and
verify power (See Warning).
If good, replace Laser Head
(PL-10). If not, replace Laser
Power Supply (PL-2).

Problem
Corrected
?

- Clean, debubble, and wet


Optical Flow Cell (VP-44),
(VP-45).
- Replace Optical Flow Cell
(PL-10).

- Replace In-Line Filter (PL-4.2).

NO

- Clean Laser Bench (VP-43).

'DQJHU=#+LJK#9ROWDJH#00#$VVXUH#WKH#SRZHU#WR#WKH#
'DQJHU
LQVWUXPHQW#LV#RII1#$#KLJK#YROWDJH#FKDUJH#PD\#UHPDLQ#RQ#
WKH#ODVHU#FRQQHFWRU#ZLWK#WKH#SRZHU#RII1#'R#QRW#WRXFK#WKH#
PDOH#SLQV1#6KRUW#PDOH#SLQV#WR#&KDVVLV1

YES
Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 24

5#7URXEOHVKRRWLQJ IP-6 Optical Parameter Count Problems


IP-6 Optical Parameter Count Problems (Page 11 of 11)

18
- From Main Menu,
press

- From Main Menu, press


DIAGNOSTICS

DIAGNOSTICS

- Verify operation of Valve 54 (VP-10)


and tubing . Replace as necessary
(PL-4.1) , (TM-1) .
- Clean Shear Valve (VP-48) .

F12

F12

- Verify RBC/PLT Sample is being


staged to Optical Flow Cell.

F1

F1

NO

SET POINT ENTRY

SET POINT ENTRY

- Verify RBC/PLT
Threshold is 550.

- Verify WBC Threshold is


360.
- Replace MAM (PL-12) .
- Replace SPM (PL-10) .

19
NO
- Verify operation of WBC
Syringe (VP-6), (VP-7).

- Replace CPU/DCM (PL-13) .


20

- Clean Shear Valve (VP-48) .


Problem
Corrected

- Verify NOC Sample is being staged


to Optical Flow Cell.

?
YES
Total Call

Problem
Corrected
?
YES

- Verify operation of Valve 41 (VP-10)


and tubing. Replace as necessary
(PL-4.1) , (TM-1) .
- From Main Menu, press
DIAGNOSTICS
F12
F1
SET POINT ENTRY

Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

- Verify NOC Threshold is 400.

Part Number 9140300 August 1998

2 - 25

5#7URXEOHVKRRWLQJ IP-7 Reagent Empty


IP-7 Reagent Empty (Page 1 of 5)

Start

- Verify reagents, type and


expiration.
- Ensure reagent
containers are connected
to the correct tubing and
input ports.
- Ensure sinkers are on
lines and lines are at
bottom of containers.
- Ensure reagent lines are
not crimped or clogged.

HGB Lyse
Empty
?
NO

YES

YES

- Open Right Front Door


and observe HGB/
WBC Syringe.

WBC Lyse
Empty
?
NO

- INITIALIZE and PRIME


instrument.
Diluent/Sheath
Empty

YES

- Open Left Front Door


and observe Diluent and
Sheath Reservoirs.

?
NO

Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 26

5#7URXEOHVKRRWLQJ IP-7 Reagent Empty


IP-7 Reagent Empty (Page 2 of 5)

From Main Menu,


press

YES

Syringe
Filling
Properly
?

RUN
CLEAR FAULT

Syringe
Moving
Properly
?

NO

- Verify operation and tubing


in valves 28 for HGB, 23
for WBC. (VP-10) See
Figure. Replace as
necessary (PL-4.1), (TM-1).

YES
- Remove Top Cover and
observe Reagent Sensor
Board. See Figure
- Inspect and seat
connectors on J1 of
Reagent Sensor Board
and J26 of FCM.
- Replace FCM (PL-14.1).

- Replace SDM2 (PL-15)


Floss Tubing
Up and Down
Ensure Tubing
is Seated in Slot

NO

- Clean/replace syringe (PL-7).


- Verify Syringe Driver
operation (VP-6), (VP-7).
- Replace Syringe Driver
Assembly (PL-7), (RR-11).

- Verify operation and tubing in


valves 24 for HGB, 25 for WBC.
(VP-10) See Figure. Replace as
necessary (PL-4.1), (TM-1).
- Replace SDM2 (PL-15).

32098

- Replace MPM (PL-11).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 27

5#7URXEOHVKRRWLQJ IP-7 Reagent Empty


IP-7 Reagent Empty (Page 3 of 5)

Diluent
Not Filling
Properly
?

From Main Menu, press


RUN
CLEAR FAULT
- Observe the Diluent and Sheath
Reservoirs and verify that both fill to
the Sensor Electrodes and
immediately stop. See Figure

YES

NO

NO

Both
Filling
Properly
?

YES

Note: The "Diluent/Sheath Empty"


message can be generated by the In-line
Diluent/Sheath Sensor, the Diluent
Reservoir Level Sensor, or the Sheath
Reservoir Level Sensor.

Sheath
Not Filling
Properly
?
NO

YES

NO

Neither
Filling
Properly
?
YES

Diluent Filling
Past Electrode
?

YES

YES

NO

Fill
To
H e re

Sheath Filling
Past Electrode

32099

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 28

5#7URXEOHVKRRWLQJ IP-7 Reagent Empty


IP-7 Reagent Empty (Page 4 of 5)

- Remove Top Cover and


observe Reagent Sensor
Board. See Figure
- Inspect and seat
connectors on J1 of
Reagent Sensor Board
and J26 of FCM.
- Replace FCM (PL-14.1).

- Ensure Diluent/Sheath plumbing


is free of restrictions.

- Ensure Vac 1 line to reservoir


is free of restrictions.

- Ensure there is no saline bridge at


reservoir connectors.

- Ensure there is no saline


bridge at reservoir connector.

- Verify the Vac 1 level (VP-16)


and verify Vac 1 retention (VP15).

- Verify operation of Valves 62


and 63 (VP-10). Replace as
necessary (PL-4.1)

- Ensure Vac 1 lines to reservoirs is


free of restrictions.

- Verify operation of Check


Valve Replace as necessary
(TM-1).

- Verify operation of Valves 61, 62,


63, and 64 (VP-10). Replace as
necessary (PL-4.1).
- Verify operation of Check Valves.
Replace as necessary (TM-1).

- Verify Reservoir is not


cracked or leaking Replace
as necessary (PL-4.2).
- Replace FCM (PL-14.1).

- Ensure Vac 1 line to


reservoir is free of
restrictions.
- Ensure there is no saline
bridge at reservoir
connector.
- Verify operation of Valves 61
and 64 (VP-10). Replace as
necessary (PL-4.1).
- Verify operation of Check
Valve. Replace as
necessary (TM-1).
- Verify Reservoir is not
cracked or leaking. Replace
as necessary (PL-4.2).
- Replace FCM (PL-14.1).

- Verify Reservoirs are not cracked


or leaking. Replace as necessary
(PL-4.2).
- Replace FCM (PL-14.1).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 29

5#7URXEOHVKRRWLQJ IP-7 Reagent Empty


IP-7 Reagent Empty (Page 5 of 5)

- Clean sensor contacts


(Electrodes and
connector) at top of
Reservoir.
- Remove pins 1 and 4
and clean and crimp
metal contacts so they
are touching. See Figure
- If pins 1 and 4 are
damaged, replace with
pins 2 and 3.

32100

- Reseat Sensor Cables


(CDM 2 J8 for Diluent, or
CDM 2 J10 for Sheath.
- Replace FCM (PL-14.1).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 30

5#7URXEOHVKRRWLQJ IP-8 Optical Backgrounds Too High (Page 1 of 1)


IP-8 Optical Backgrounds Too High (Page 1 of 1)
Start
- Perform the following in order and verify
backgrounds after each action taken.

- From Main Menu, press


- Verify Background Counts
(VP-56).

DIAGNOSTICS
NO

- Perform Extended Autoclean.

F12

- Clean Shear Valve (VP-48).

F1

- Clean and debubble Optical Flow Cell


(VP-44).

SET POINT ENTRY


WBC Only
Too High
?

- Verify RBC/PLT Threshold is 550.


- From Main Menu, press

- Clean/replace Diluent and Sample


Injection Syringes (PL-7).

SPECIAL PROTOCOLS

- Replace In-line Sheath Filter (PL-4.2).

MORE
YES

NO

REAGENT RESERVOIR
- Follow on-screen instructions and press

- Ensure RBC/PLT Mixing Chamber is


being rinsed and drained properly.
- Clean Laser Bench (VP-43).

EMPTY DIL/SHEATH
- From Main Menu, press

- Replace Diluent Container and press

DIAGNOSTICS

FILL DIL/SHEATH

F12

- Run multiple backgrounds and verify.

F1
SET POINT ENTRY
- Verify WBC Threshold is 360.
- Replace WBC Lyse Container.
- Clean/replace WBC Syringe (PL-7).

Backgrounds
OK

- Ensure WBC Mixing Chamber is


being rinsed and drained properly.

- INITIALIZE and PRIME Instrument.

YES
Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 31

5#7URXEOHVKRRWLQJ IP-9 Histogram Problems (Page 1 of 1)


IP-9 Histogram Problems (Page 1 of 1)

Start
- Perform the following in order
and verify histograms after
each action taken.

- Verify reagents; type and


expiration date.
- Ensure reagents are at
room temperature.
- Verify Backgrounds (VP-56).

WBC Only

- Ensure RBC/PLT and/or


WBC Mixing Chambers are
being rinsed and drained
properly.

NO

- Perform Extended Autoclean.


- Clean Shear Valve (VP-48).

Backgrounds
OK

NO

Go to
(IP-8) N O

- Clean, debubble, and wet


Optical Flow Cell (VP-44),
(VP-45).

NO

- Fine align Optical Flow Cell


(VP-20).

YES

YES

- Verify WBC Gains


(VP-23).

- Verify 0 o and 10 o Log


Amplifiers (VP-28).
- Verify 0 o and 10 o Gains
(VP-26), (VP-27).

- Clean Laser Bench (VP-43).

- Replace WBC Lyse for WBC


problem or Diluent for RBC/
PLT problem.

- Clean/replace Diluent, WBC


Lyse, and Sample Injection
Syringes (PL-7).
- Replace In-line Sheath Filter
(PL-4.2).

Problem
Corrected
?
YES
Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Problem
Corrected
?
YES

Total Call

Part Number 9140300 August 1998

2 - 32

5#7URXEOHVKRRWLQJ IP-10 Controls Out


IP-10 Controls Out (Page 1 of 2)

Start

NO

- Verify reagents; type and


expiration date.
- Ensure reagents are at room
temperature and have not
been previously frozen.
- Ensure Controls and
Calibrators have not been
frozen or heat stressed.

Q.C. OK

YES

?
Total Call
- Perform Autoclean.
- From Main Menu, press
SPECIAL PROTOCOLS

- Open fresh Commercial


Control, and run Q.C.

MORE
AUTOCLEAN
- Verify Backgrounds (VP-56).

- Check Control Lot #


- Use current Control Insert
Sheet to verify Q.C means/
limits are correct if customer
has not established their own
means.
- From Main Menu, press
SETUP
Q.C. SETUP MENU
Q.C. LIMITS
- Edit as necessary.
- Run Q.C.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

YES

All
Backgrounds
OK
?
NO

Results
Acceptable

NO

?
YES
Total Call

Go to (IP-8).

Part Number 9140300 August 1998

2 - 33

5#7URXEOHVKRRWLQJ IP-10 Controls Out


IP-10 Controls Out (Page 2 of 2)

NO
- Clean Shear Valve (VP-48).

Precision
In Spec
?

- Verify System Precision


- From Main Menu, press
RUN
SPECIMEN TYPE

YES
- Recalibrate out-ofrange parameter(s)
(VP-58).

- Determine which
parameter(s) are out-ofrange, and go to (IP-15)
or (IP-16) for HGB and
correct.
- For indices (MCH or
MCHC), determine which
parameter (HGB, MCV,
or RBC is imprecise and
troubleshoot accordingly.
MCH = HGB X10
RBC

- Select empty Q.C. File


Q.C. SPECIMEN
- Review CV% (VP-57).

MCHC = HGB X 100


MCV X RBC

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 34

5#7URXEOHVKRRWLQJ IP-11 Vacuum Accumulator #1 Wet


IP-11 Vacuum Accumulator #1 Wet (Page 1 of 2)

Start

- Reinitialize Analyzer
From Main Menu:

Liquid Exiting
Analyzer

YES

DIAGNOSTICS
MORE
INITIALIZATION

NO

- Verify that Waste Chamber 2 is


not overflowing. If overflowing,
verify Pressure Valve 18 (VP-10).
Replace as necessary (PL-4.1).
Check output Check Valve.
Replace as necessary (TM-1).
- Ensure operation of Waste
Chamber 2 Trap and Trap Output
Check Valve. Replace as
necessary (PL-4.2) and (TM-1).

- Perform Vacuum
Accumulator #2 Rinsing
Procedure (VP-60)
Press:
MORE
- Observe the Waste Line into
External Waste Container and
press:
DRAIN ACCUMULATORS

Problem
Corrected

NO

?
YES

- Perform Vacuum
Accumulator #2 Rinsing
Procedure (VP-60)

Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 35

5#7URXEOHVKRRWLQJ IP-11 Vacuum Accumulator #1 Wet


IP-11 Vacuum Accumulator #1 Wet (Page 2 of 2)

1
- Check Cable and replace
if necessary (PL-5)
- Remove connector from
J11 on VPM and check
resistance between
connector pins. Must be
greater than 20
Megohms. See Figure

- Replace Accumulator
(PL-5)

NO
J10
Vac 1 Acc

J11
Vac 2 Acc

VPM
BD

> 20 Megohms
?
YES

32003

- Replace VPM (PL-5)


- Replace FCM (PL-14.1)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 36

5#7URXEOHVKRRWLQJ IP-12 Vacuum Accumulator #2 Wet


IP-12 Vacuum Accumulator #2 Wet (Page 1 of 2)

Start

- Reinitialize Analyzer
From Main Menu:

Liquid Exiting
Analyzer

YES

DIAGNOSTICS
MORE
INITIALIZATION

NO

- Verify that Waste Chamber 2 is


not overflowing. If overflowing,
verify Pressure Valve 18 (VP-10).
Replace as necessary (PL-4.1).
Check output Check Valve.
Replace as necessary (TM-1).
- Ensure operation of Waste
Chamber 2 Trap and Trap Output
Check Valve. Replace as
necessary (PL-4.2) and (TM-1).

- Perform Vacuum
Accumulator #2 Rinsing
Procedure (VP-60)
Press:
MORE
- Observe the Waste Line into
External Waste Container and
press:
DRAIN ACCUMULATORS

Problem
Corrected

NO

?
YES

- Perform Vacuum
Accumulator #2 Rinsing
Procedure (VP-60)

Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 37

5#7URXEOHVKRRWLQJ IP-12 Vacuum Accumulator #2 Wet


IP-12 Vacuum Accumulator #2 Wet (Page 2 of 2)

1
- Check Cable and replace
if necessary (PL-5)
- Remove connector from
J11 on VPM and check
resistance between
connector pins. Must be
greater than 20
Megohms. See Figure

- Replace Accumulator
(PL-5)

NO
J10
Vac 1 Acc

J11
Vac 2 Acc

VPM
BD

> 20 Megohms
?
YES

32003

- Replace VPM (PL-5)


- Replace FCM (PL-14.1)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 38

5#7URXEOHVKRRWLQJ IP-13 HGB Problems


IP-13 HGB Problems (Page 1 of 2)

Start

"0", Low, High, or


Erratic
- Verify proper Reagents and
Expiration Date.

HGB Only

From Main Menu, Press:


RUN
SPECIMEN TYPE
FRAGILE WBC

Both

- Run a Normal Control five


times and record the NOC
and HGB values.
- Compare the NOC and HGB
results to the Data Sheet.

- Perform HGB Cleaning


Procedure (VP-49).
- Remove, inspect, and clean Shear
Valve.
- Perform Vacuum & Pressure Level
Verification/Adjustment (VP-16).
- Verify tubing and operation of Valves
51, 28, 24, 33, 93, and 26 (VP-10)
and replace as necessary (PL-4.1).

From Main Menu, Press:


DIAGNOSTICS
RAW DATA SUMMARY
- Run five background counts
and observe the HGB Sample
and Reference Readings.

- Verify operation of HGB Lyse and


RBC Diluent Syringes (VP-6), (VP-7)
and replace as necessary (PL-7).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 39

5#7URXEOHVKRRWLQJ IP-13 HGB Problems


IP-13 HGB Problems (Page 2 of 2)

Total Call
Select path from Chart below.
Reference

Sample
2

1950-2050

1950-2050

< 1950

< 1950

> 2050

>2050

Erratic

1950-2050

1600

1950-2050

Erratic

Erratic

- Verify connector at J18 of FCM.


3

- Replace HGB Flow Cell (PL-4.2).


- Replace FCM (PL-14.1).
- Replace CPU/DCM (PL-13).

- Perform HGB Adjustment


(VP-5)

5
- Verify tubing, operation (VP-10),
and flow through Valve 94 and
replace as necessary (TM-1),
(PL-4.1).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 40

5#7URXEOHVKRRWLQJ IP-14 X-B Errors


IP-14 X-B Errors (Page 1 of 3)

Effect Of RBC, MCV, & HGB On X-B Parameters

Start

- Clean Fan Filters and verify all Fans are running


properly.
- Ensure there is at least 6" of clearance behind and
on the left side of Instrument.

X-B Parameter

RBC

RBC

MCV

NA

NA

MCH

NA

- Review patient result in Datalog, and look for groups


( 3 or more) of abnormal MCV, RBC, or HGB.
-If MCV shifts, ask Customer for possible causes,
such as Oncology or Geriatric Patients.
If MCH or MCHC errors:
- See table to determine which parameter (HGB,
RBC, MCV) is bad.
Note: HCT = RBC X MCV/10

MCV

HGB

HGB

NA

NA

NA

MCHC

- Ensure Instrument is not subject to drastic


temperature variations (direct sunlight, heating/air
conditioning ducts).
- If Fan Filters were clogged and then cleaned, allow a
15 minute temperature stabilization period.

MCV

Controls at or
Near 2 SD
of mean
?

NO

- Review Customer Maintenance Log.


- Review Maintenance Procedures and
Dates.
- Review X-B Results.
- Determine if Maintenance was
performed just before X-B Shift.

YES
Go to (IP-10)
Controls Out.

MCH = HGB X 10/RBC


MCHC = (HGB X 100)/(RBC X MCV)

- Review Q.C. Logs for all 3 Control Levels.


- Verify RBC, HGB, and MCV Control Levels are not
at the high or low end of range.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 41

5#7URXEOHVKRRWLQJ IP-14 X-B Errors


IP-14 X-B Errors (Page 2 of 3)

1
- Verify Reagent, Type and
Expiration Date.
Shift
After
Maintenance
?
Reagent
Change
Required
?

YES
- Repeat Maintenance
Procedures
performed before X-B
Shift.
- Monitor X-B for 5 Data
Points (100 Samples).

NO
X-B In
Range

YES
- Monitor X-B for 5
Data Points (100
Samples).

?
YES
X-B In
Range

Total Call

NO

?
YES

Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 42

5#7URXEOHVKRRWLQJ IP-14 X-B Errors


IP-14 X-B Errors (Page 3 of 3)

- Verify System Precision


- From Main Menu, press
RUN
SPECIMEN TYPE
- Select empty Q.C. File
Q.C. SPECIMEN
- Run one Patient 10
times.
- Review CV% (VP-57) .

All
Parameters
OK
?

NO

- Determine which
parameter(s) are out-ofrange, and go to (IP-15) or
(IP-16) for HGB and correct.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

YES
- Verify Calibrator Insert
Sheet matches Current
Calibrators.
- Perform System
Calibration (VP-58) .

Part Number 9140300 August 1998

2 - 43

5#7URXEOHVKRRWLQJ IP-15 Optical Parameters Imprecision


IP-15 Optical Parameters Imprecision (Page 1 of 3)

Start
- Verify Background Counts
(VP-56).

- Clean Fan Filters and verify all Fans are


running properly.
- Ensure there is at least 6" of clearance
behind and on the left side of Instrument.
- Ensure Instrument is not subject to drastic
temperature variations (direct sunlight,
heating/air conditioning ducts).
- If Fan Filters were clogged and then
cleaned, allow a 15 minute temperature
stabilization period.
- Perform Autoclean
From Main Menu, press

- Verify System Precision.


- From Main Menu, press
SPECIMEN TYPE
- Select empty Q.C. File
- Run one Patient 10 times.
- Review CV% (VP-57).

- Manually clean, debubble, and wet Optical


Flow Cell (VP-44), (VP45).
- Clean Optics Bench (VP-43).

Go to (IP-8).

YES

YES

All
Imprecise

NO
Problem
Corrected
?

RBC/PLT
Only
?

YES

- Replace In-line Sheath Filter (PL-4.2).


Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

NO

YES

MORE
AUTOCLEAN

?
NO

Q.C. SPECIMEN

SPECIAL PROTOCOLS

- Clean Shear Valve (VP-48).

Backgrounds
OK

RUN

NO
1

Part Number 9140300 August 1998

2 - 44

5#7URXEOHVKRRWLQJ IP-15 Optical Parameters Imprecision


IP-15 Optical Parameters Imprecision (Page 2 of 3)

2
- Replace WBC Lyse Container.
YES

F12
F1
SET POINT ENTRY

WBC
Only
?

- Verify WBC Threshold is 360.


- Ensure WBC Mixing Chamber is
being rinsed and drained
properly.
- Verify operation of WBC Lyse
Syringe (VP-6) , (VP-7) .
Troubleshoot as necessary.
- Verify/adjust WBC Gain (VP-23) .

NO

NOC
Only
?

- Replace Shear Valve Sections


(PL-9.1) .

YES
- Ensure HGB Mixing
Chamber is being rinsed
and drained properly.

- From Main Menu, press


DIAGNOSTICS

NO

- Replace MAM (PL-12) .


- Replace SPM (PL-10) .

- Verify samples are being staged to


Optical Flow Cell by Peri-pump.
Troubleshoot as necessary.
- Verify Sheath Reservoir is filling
properly.
- Verify Pressure 2 (VP-16) and
proper Sheath flow to Optical Flow
Cell. Troubleshoot as necessary.
- Verify operation of Sample
Injection Syringe (VP-6) , (VP-7 ).
Troubleshoot as necessary.
- Replace Optical Flow Cell (PL-10) .
- Replace Shear Valve Sections
(PL-9.1) .
- Replace MAM (PL-12) .
- Replace SPM (PL-10) .
- Replace CPU/DCM (PL-13) .

- Replace CPU/DCM (PL-13) .

Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 45

5#7URXEOHVKRRWLQJ IP-15 Optical Parameters Imprecision


IP-15 Optical Parameters Imprecision (Page 3 of 3)

- Replace Diluent Container.


- From Main Menu, press
DIAGNOSTICS
F12
F1
SET POINT ENTRY
- Verify RBC/PLT Threshold is 550.
- Ensure RBC/PLT Mixing Chamber is
being rinsed and drained properly.
- Verify 0 o and 10 o Log Amplifiers (VP-28).
- Verify 0 o and 10 o Gain (VP-26), (VP-27).
- Replace Shear Valve Sections (PL-9.1).
- Replace MAM (PL-12).
- Replace SPM (PL-10).
- Replace CPU/DCM (PL-13).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 46

5#7URXEOHVKRRWLQJ IP-16 HGB Imprecision (Page 1 of 1)


IP-16 HGB Imprecision (Page 1 of 1)

Start

- Clean Fan Filters and verify all Fans are


running properly.
- Ensure there is at least 6" of clearance
behind and on the left side of
Instrument.
- Ensure Instrument is not subject to
drastic temperature variations (direct
sunlight, heating/air conditioning ducts).
- If Fan Filters were clogged and then
cleaned, allow a 15 minute temperature
stabilization period.

HGB CVs
OK

- Ensure HGB Flow Cell is being


rinsed, drained, and vented
properly. Verify Valves 51, 94, 93,
(VP-10) and tubing . Replace as
necessary (PL-4), (TM-1).

?
YES
NO

- Clean HGB Lyse Syringe.

Total Call
NO
- Replace HGB Lyse
Container>

- Verify HGB Lyse Syringe (VP-6),


(VP-7). Replace as necessary
(PL-7).
- Inspect and re-seat Cable at J18
of FCM (PL-4.2).

- Clean Shear Valve (VP-48).

- Replace HGB Flow Cell (PL-4.2).

- Manually clean HGB Flow Cell (VP-49).

- Replace FCM (PL-14.1).

- Verify System Precision.

NO

- Replace CPU/DCM (PL-13).

- From Main Menu, press


RUN
SPECIMEN TYPE
- Select empty Q.C. File
Q.C. SPECIMEN
- Run one Patient 10 times.

HGB CVs
OK
?
YES

- Review HGB CV% (VP-57).


Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 47

5#7URXEOHVKRRWLQJ IP-17 Primary Vacuum/Pressure Isolation,

5
4

YES
3

NO

Vac
Constantly
Running

NO

From Main Menu:


DIAGNOSTICS
MORE
INITIALIZATION

- Reinitialize Analyzer

9P SI

YES

12P SI

- Remove Left Side Cover and


extend Air Supply (RR-7) to
observe pumps. (Vac-Front,
Pres-Rear)

TO
FLO W PA N E L

To S L

Pres
Constantly
Running

To S L

Start

5P SI 12"H g 8"Hg 12P SI 12"H g

IP-17 Primary Vacuum/Pressure Isolation, (Page 1 of 9)

3 2 00 4

NO
Both
Constantly
Running

- Disconnect tubing from


positions 6 and 7 on Air
Supply Manifold, and
observe Pumps and VPM
Pump LEDs. See Figures

?
YES

32005

- Observe LEDs. See


Figure

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 48

5#7URXEOHVKRRWLQJ IP-17 Primary Vacuum/Pressure Isolation,


IP-17 Primary Vacuum/Pressure Isolation, (Page 2 of 9)

1
All Valves
Close Then
Open
?

Both LEDs
ON

NO
2

+12 VNR
Fuse (Top)

YES

NO
- Replace Pump Relay
Board (PL-5)

32006

- Check +12V UR and Fuse.


See Figure

YES

- Replace if necessary (PL-1)

Check fuse again

NO
- Reinitialize Analyzer
From Main Menu:
DIAGNOSTICS
MORE

Problem
Corrected

Fuse
Blown

INITIALIZATION
- Observe Flow Panel
valves

NO

YES
Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

YES

- Replace Power Supply


(PL-1)

Part Number 9140300 August 1998

2 - 49

5#7URXEOHVKRRWLQJ IP-17 Primary Vacuum/Pressure Isolation,


IP-17 Primary Vacuum/Pressure Isolation, (Page 3 of 9)

2
Note: If the +24 UR is approximately
the same as the +12 UR, the +24V
Fuse is blown or the +24V Power
Supply is defective.

- Check +24V UR (28.9V37.7V) and Fuse


(Bottom Fuse)
- While observing VPM
Solenoid LEDs (See
Figure) press:
INITIALIZATION

- Replace Fuse if
necessary (PL-1)
NO
NO

- Check Fuse again


Problem
Corrected
LEDs Bright
Then Dim When
Activated

YES

Fuse
Blown

YES

?
Total Call

YES

NO

- Replace Power Supply


(PL-1)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

32007

2 - 50

5#7URXEOHVKRRWLQJ IP-17 Primary Vacuum/Pressure Isolation,

TO
FL O W PA N E L

P inch

Pumps
Stop
?

NO

12P SI

9P SI

YES

- Pinch tubing at positions


6 and 7 that supply 12
psi and 12" Hg to Sample
Loader

- Pinch tubing as shown in


Figure

- Unpinch tubing

5P SI 12"H g 8"H g 12P SI 12"H g

To S L

To S L

IP-17 Primary Vacuum/Pressure Isolation, (Page 4 of 9)

32008

Pumps
Stop

NO

?
- Inspect 12 psi and 12" Hg
plumbing and
Accumulators. Replace
as necessary (PL-5),
(TM-2)
- Inspect Vent Valves 1 and
4. Replace as necessary
(PL-5)
- Replace VPM (PL-5)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

YES

- Inspect Sample Loader


plumbing and 3-Way
Valves. Replace as
necessary (PL-18.3),
(TM-3)

- Ensure Valves 31 and 32,


97 and 98, 17 and 18 are
not open at the same
time.
- Inspect Flow Panel 12 psi
and 12" Hg plumbing for
leaks.

Part Number 9140300 August 1998

2 - 51

5#7URXEOHVKRRWLQJ IP-17 Primary Vacuum/Pressure Isolation,


IP-17 Primary Vacuum/Pressure Isolation, (Page 5 of 9)

4
Pump
Stops

YES

- Unpinch tubing and pinch


at position 6 on Manifold.

- Observe Pressure Pump


LED. See Figure Page 1
NO

Pump
Stops
?

YES

- Replace VPM (PL-5)

To S L
To S L

9P S I

P inch
1

- Pinch tubing See Figure


this page

- Inspect Sample Loader


plumbing and 3-Way
Valves. Replace as
necessary (PL-18.3),
(TM-3)

- Replace Pump Relay


Board (PL-5)

TO
FL O W PA N E L

NO

YES

- Inspect Flow Panel 12 psi


plumbing for leaks.

5P S I 12"H g 8"H g 12P S I 12"H g

- Inspect Vent Valve 1.


Replace as necessary
(PL-5)

12P S I

LED
ON

NO

- Inspect 12 psi plumbing


and Accumulator.
Replace as necessary
(PL-5), (TM-2)

32 0 09

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 52

5#7URXEOHVKRRWLQJ IP-17 Primary Vacuum/Pressure Isolation,


IP-17 Primary Vacuum/Pressure Isolation, (Page 6 of 9)

5
Pump
Stops

YES

- Unpinch tubing and pinch


at position 7 on Manifold.

- Observe Vacuum Pump


LED. See Figure Page 1
NO

Pump
Stops
?

- Inspect 12" Hg plumbing


and Accumulator.
Replace as necessary
(PL-5), (TM-2)

- Replace VPM (PL-5)

To S L
To S L

9P SI

Pinch

- Pinch tubing. See Figure

- Inspect Sample Loader


plumbing and 3-Way
Valves. Replace as
necessary (PL-18.3),
(TM-3)

- Replace Pump Relay


Board (PL-5)

TO
FLO W PAN E L

YES

YES
5P SI 12"H g 8"H g 12P SI 12"H g

NO

- Inspect Flow Panel 12"


Hg plumbing for leaks.

12P SI

- Inspect Vent Valve 4.


Replace as necessary
(PL-5)

LED
ON

NO

32010

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 53

5#7URXEOHVKRRWLQJ IP-17 Primary Vacuum/Pressure Isolation,


IP-17 Primary Vacuum/Pressure Isolation, (Page 7 of 9)

- Replace VPM
(PL-5)

NO

- Reseat connectors VPM J18, PRM


J1, J2, J3, J4
Neither
Pump
Runs

YES

LEDs
ON

- Replace VPM
(PL-5)

NO
NO

YES

NO

Pres
Doesn't
Run

VAC LED
ON

NO

- Replace PRM (PL-xx)

- Reseat connectors VPM J18, PRM


J1, J3
YES

- Check Fuse on PRM. Replace if


necessary (PL-5)
- Verify 115VAC to PRM

VAC
Doesn't
Run

YES

- Check 115VAC across Pins 1 and 2


on PRM P3. IF present, replace Vac
Pump. If not, replace PRM (PL-5)

- Replace VPM
(PL-5)
- Reseat connectors VPM J18,
PRM J1, J2

YES

Pres LED
ON
?

YES

- Check 115VAC across Pins 1 and


2 on PRM P2. IF present, replace
Pres Pump. If not, replace PRM
(PL-5)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 54

5#7URXEOHVKRRWLQJ IP-17 Primary Vacuum/Pressure Isolation,


IP-17 Primary Vacuum/Pressure Isolation, (Page 8 of 9)

7
NO

Both
OK

- Re-connect tubing to
positions 6 and 7 (6-Red,
7-Green)

- Replace Pump Head


- If problem persists,
replace Pump Assembly

Pump Head:
Pressure (PL-5)

YES

Vacuum

(PL-5)

Pump Assy:
- Perform Vacuum and
Pressure Level
Verification/Adjustment
(VP-16)

- Observe Vacuum and


Pressure Pumps and
time the duration between
run cycles.

- Perform Vacuum and


Pressure Recovery
Verification (VP-17)

Pres
Cycles
< 2 min
YES

Pressure (PL-5)
Vacuum

NO

Vac
Cycles
< 2 min

(PL-5)

NO

Total Call
See Pressure Leaks (IP-18)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

See Vacuum Leaks (IP-19)

YES

Part Number 9140300 August 1998

2 - 55

5#7URXEOHVKRRWLQJ IP-17 Primary Vacuum/Pressure Isolation,


IP-17 Primary Vacuum/Pressure Isolation, (Page 9 of 9)

To
C D M 2-J3
FC M -J5

- If the Fuse continues to blow,


assemblies on the +12V UR or
+24V UR must be disconnected
one at a time until the shorted
assembly is found and
replaced. (See Figures)
- Power-down instrument and
connect Meter between TP2+12V or TP1-+24V and TP4 on
PSM and remove connectors
until short is found
- Replace defective assembly and
verify proper operation

To
S D M /C D M -J3

J1
UR RET
1
3
4 +1 2 V U R
5 +2 4 V U R
J2
UR RET
1
3
4 +1 2 V U R
+2 4 V U R
5

B a ck

C D M 2 & S D M /C D M P o w er C on n ecto rs
J1
J3
+2 4 V U R
5
1
Fro m
+1 2 V U R
To
4
2
P.S .
SDM A
UR RET
J1/J2 3
3
1

PSM

J2
1
2
3

J3
To
S h ea r
Valve
D rive r-J3

+2 4 V U R
1
2 RET
N o te: J4 an d J5 a re no t o n
S D M /C D M

To
S D M 9 -J1 0

J4
+2 4 V U R
1
+1 2 V U R
2
3 UR RET

J4
1
2

To
SDM C

3
FC M
J5
1
2
3 2 0 11

CELL-DYN 3200 SYSTEM SERVICE MANUAL

To
SDM B

Part Number 9140300 August 1998

To
SDM D

2 - 56

5#7URXEOHVKRRWLQJ IP-18 Pressure Leak Isolation


IP-18 Pressure Leak Isolation (Page 1 of 7)
Note: This procedure is designed to isolate leaks in the pressure plumbing. Refer to IP-17 Primary Vacuum/
Pressure Isolation for troubleshooting gross pressure failures.
Start

- Remove Left Side Cover and


extend Air Supply (RR-7) to
observe Pres Pump. (VacFront, Pres-Rear)

- Reinitialize Analyzer
From Main Menu:
DIAGNOSTICS
MORE

- Observe Pressure Pump


and time the duration
between run cycles.

Pump
Cycles
< 2 min

YES

INITIALIZATION
NO

Total Call
- Adjust Pressure Levels if
necessary (VP-16)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 57

5#7URXEOHVKRRWLQJ IP-18 Pressure Leak Isolation


IP-18 Pressure Leak Isolation (Page 2 of 7)

1
- Check +24V UR (28.9V37.7V) and Fuse
(Bottom Fuse)
- While observing VPM
Solenoid LEDs (See
Figure) press:
INITIALIZATION

- Replace Fuse if
necessary (PL-1)
NO

Note: If the +24 UR is approximately


the same as the +12 UR, the +24V
Fuse is blown or the +24V Power
Supply is defective.

NO
- Check Fuse again
Problem
Corrected

LEDs Bright
Then Dim When
Activated

YES

Fuse
Blown

YES

?
Total Call

YES

NO

- Replace Power Supply


(PL-1)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 2 0 07

2 - 58

5#7URXEOHVKRRWLQJ IP-18 Pressure Leak Isolation


IP-18 Pressure Leak Isolation (Page 3 of 7)

- Select 3 pressure levels in


real-time press:
MORE
VOLTAGE READINGS
Move cursor to each level
and press:
SELECT
When finished press:
FINISH SELECT

- Observe the 3 levels and


verify that none decrease
more than 0.2 psi in 1
minute.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 59

5#7URXEOHVKRRWLQJ IP-18 Pressure Leak Isolation


IP-18 Pressure Leak Isolation (Page 4 of 7)
3

Pres
3
OK

- Inspect tubing between


Air Supply and Flow
Panel.
YES

?
NO
NO
To S L

YES

Pres
1
OK
?

- Inspect 5 psi Accumulator


and plumbing on Air
Supply. Replace as
necessary (PL-5), (TM-2)

9P SI

NO

12P SI

To S L

TO
FL O W PA N E L

Pres
2
OK

- Inspect Diluent Reservoir


for leaks. Replace as
necessary (PL-4.2).

5P SI 12"H g 8"H g 12P SI 12"H g

Pres
3
OK

YES

- Inspect plumbing through


Valve 63 to Diluent
Reservoir.

NO

- Pinch tubing at position 3


of Air Supply Manifold.
See Figure

32004

YES

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Total Call

Part Number 9140300 August 1998

2 - 60

5#7URXEOHVKRRWLQJ IP-18 Pressure Leak Isolation


IP-18 Pressure Leak Isolation (Page 5 of 7)

- Inspect tubing between


Air Supply and Flow
Panel.

- Pinch tubing at position 2


of Air Supply Manifold.
See Figure Page 2.
YES

- Pinch tubing See Figure

- Inspect plumbing through


Valve 64 to Sheath
Reservoir.
- Inspect Sheath Reservoir
for leaks. Replace as
necessary (PL-4.2).

YES

P inch

NO

- Inspect Valve 1. Replace


as necessary (PL-5).
- Inspect 12 psi plumbing
and Accumulator.
Replace as necessary
(PL-5), (TM-2).

12P SI

9P SI

NO

TO
FL O W PA N E L

To S L

5P SI 12"H g 8"H g 12P SI 12"H g

To S L

Pres
2
OK

- Inspect 9 psi Accumulator


and plumbing on Air
Supply. Replace as
necessary (PL-5), (TM-2)

Pres
1
OK

32009

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 61

5#7URXEOHVKRRWLQJ IP-18 Pressure Leak Isolation


IP-18 Pressure Leak Isolation (Page 6 of 7)

6
- Inspect plumbing to
Valves 32, 98, and 18.
- Unpinch tubing and pinch
at position 6 on Manifold.

- Press in on Valves 32, 98,


and 18 one at a time, if
leaking stops replace as
NO
necessary (PL-4.1)

Pres
1
OK
?

YES

- Inspect Sample Loader


plumbing and 3-Way
Valves. Replace as
necessary (PL-18.3),
(TM-3)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 62

5#7URXEOHVKRRWLQJ IP-18 Pressure Leak Isolation


IP-18 Pressure Leak Isolation (Page 7 of 7)

To
C D M 2-J3
FC M -J5

- If the Fuse continues to blow,


assemblies on the +12V UR or
+24V UR must be disconnected
one at a time until the shorted
assembly is found and replaced.
(See Figures)
- Power-down instrument and
connect Meter between TP2+12V or TP1-+24V and TP4 on
PSM and remove connectors
until short is found
- Replace defective assembly and
verify proper operation

To
S D M /C D M -J3

J1
UR RET
1
3
4 +1 2 V U R
5 +2 4 V U R
J2
UR RET
1
3
4 +1 2 V U R
+2 4 V U R
5

B a ck

C D M 2 & S D M /C D M P o w er C on n ecto rs
J1
J3
+2 4 V U R
1
5
Fro m
+1 2 V U R
To
4
2
P.S .
SDM A
UR RET
J1/J2 3
3
1

PSM

J2
1
2
3

J3
To
S h ea r
Valve
D rive r-J3

+2 4 V U R
1
2 RET
N o te: J4 an d J5 a re no t o n
S D M /C D M

To
S D M 9 -J1 0

J4
+2 4 V U R
1
+1 2 V U R
2
3 UR RET

J4
1
2

To
SDM C

3
FC M
J5
1
2
3 2 0 11

CELL-DYN 3200 SYSTEM SERVICE MANUAL

To
SDM B

Part Number 9140300 August 1998

To
SDM D

2 - 63

5#7URXEOHVKRRWLQJ IP-19 Vacuum Leak Isolation


IP-19 Vacuum Leak Isolation (Page 1 of 6)
Note: This procedure is designed to isolate leaks in the vacuum plumbing. Refer to IP-17 Primary Vacuum/
Pressure Isolation for troubleshooting gross vacuum failures.
Start

- Remove Left Side Cover and


extend Air Supply (RR-7) to
observe Vac Pump. (VacFront, Pres-Rear)

- Reinitialize Analyzer
From Main Menu:
DIAGNOSTICS
MORE

- Observe Vacuum Pump


and time the duration
between run cycles.

Pump
Cycles
< 2 min

YES

INITIALIZATION
NO

Total Call
- Adjust Vacuum Levels if
necessary (VP-16)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 64

5#7URXEOHVKRRWLQJ IP-19 Vacuum Leak Isolation


IP-19 Vacuum Leak Isolation (Page 2 of 6)

1
- Check +24V UR (28.9V37.7V) and Fuse
(Bottom Fuse)
- While observing VPM
Solenoid LEDs (See
Figure) press:
INITIALIZATION

- Replace Fuse if
necessary (PL-1)
NO

Note: If the +24 UR is approximately


the same as the +12 UR, the +24V
Fuse is blown or the +24V Power
Supply is defective.

NO
- Check Fuse again
Problem
Corrected

LEDs Bright
Then Dim When
Activated

YES

Fuse
Blown

YES

?
Total Call

YES

NO

5
3 2 0 07

CELL-DYN 3200 SYSTEM SERVICE MANUAL

- Replace Power Supply


(PL-1)

Part Number 9140300 August 1998

2 - 65

5#7URXEOHVKRRWLQJ IP-19 Vacuum Leak Isolation


IP-19 Vacuum Leak Isolation (Page 3 of 6)

- Select 2 vacuum levels in


real-time press:
MORE
VOLTAGE READINGS
Move cursor to each level
and press:
SELECT
When finished press:
FINISH SELECT

- Observe the 2 levels and


verify that none decrease
more than 0.2 psi in 1
minute.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 66

5#7URXEOHVKRRWLQJ IP-19 Vacuum Leak Isolation


IP-19 Vacuum Leak Isolation (Page 4 of 6)

NO

- Pinch tubing at position 5


of Air Supply Manifold.
See Figure

Vac
2
OK

- Inspect tubing between Air Supply and


Flow Panel.
- Inspect plumbing through Valve 17 to
WC-2.
YES

- Inspect WC-2 for leaks. Replace as


necessary (PL-4.2).
- Inspect plumbing to Valve 12.
- Press in on Valve 12. Replace as
necessary (PL-4.1).

Vac
2
OK

YES

- Inspect Vac 2
Accumulator and
plumbing on Air Supply.
Replace as necessary
(PL-5), (TM-2)

5
4
3

12P SI

9P SI

Total Call

YES

TO
FL O W PA N E L

To S L

5P SI 12"H g 8"H g 12P SI 12"H g

NO

To S L

NO

?
Vac
1
OK

32004

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 67

5#7URXEOHVKRRWLQJ IP-19 Vacuum Leak Isolation


IP-19 Vacuum Leak Isolation (Page 5 of 6)

4
- Unpinch tubing and
pinch at position 7 on
Manifold.

- Inspect plumbing to
Flow Panel.

- Pinch tubing See Figure


YES

NO

- Inspect WC-3 and WC4 for leaks. Replace as


necessary (PL-4.2)

Vac
1
OK

Vac
1
OK

- Press in on Valves 13,


14, 21, 36, and 37 one
at a time. Replace as
necessary (PL-4.1).

9P SI
12P SI

- Inspect Vent Valve 4.


Replace as necessary
(PL-5)

YES
TO
FL O W PA N E L

5P SI 12"H g 8"H g 12P SI 12"H g

P inch

- Inspect 12" Hg
plumbing and
Accumulator. Replace
as necessary (PL-5),
(TM-2)

To S L

NO

?
To S L

- Inspect plumbing to
Valves 31 and 97.

- Inspect Sample Loader


plumbing and 3-Way
Valves. Replace as
necessary (PL-18.3),
(TM-2)

32010

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 68

5#7URXEOHVKRRWLQJ IP-19 Vacuum Leak Isolation


IP-19 Vacuum Leak Isolation (Page 6 of 6)

To
C D M 2-J3
FC M -J5

- If the Fuse continues to blow,


assemblies on the +12V UR or +24V
UR must be disconnected one at a
time until the shorted assembly is
found and replaced. (See Figures)
- Power-down instrument and connect
Meter between TP2-+12V or TP1+24V and TP4 on PSM and remove
connectors until short is found
- Replace defective assembly and
verify proper operation

To
S D M /C D M -J3

To
S h ea r
Valve
D rive r-J3

J1
UR RET
1
3
4 +1 2 V U R
5 +2 4 V U R
J2
UR RET
1
3
4 +1 2 V U R
+2 4 V U R
5

B a ck

C D M 2 & S D M /C D M P o w er C on n ecto rs
J1
J3
+2 4 V U R
5
1
Fro m
To
+1 2 V U R
4
2
P.S .
SDM A
UR RET
J1/J2 3
3
1

PSM

J2
1
2
3

J3
+2 4 V U R
1
2 RET
N o te: J4 an d J5 a re no t o n
S D M /C D M

To
S D M 9 -J1 0

J4
+2 4 V U R
1
+1 2 V U R
2
3 UR RET

J4
1
2

To
SDM C

3
FC M
J5
1
2
3 2 0 11

CELL-DYN 3200 SYSTEM SERVICE MANUAL

To
SDM B

Part Number 9140300 August 1998

To
SDM D

2 - 69

5#7URXEOHVKRRWLQJ IP-20 Shear Valve Position Fault


IP-20 Shear Valve Position Fault (Page 1 of 2)

Start
- Check +24V

- Verify +24V UR (28.9V37.7V) (VP-1).

Fuse (Bottom
Fuse).

- Remove, inspect, and clean


Shear Valve (VP-48).

NO

- Replace Fuse if
necessary (PL-1).

Note: If the +24 UR


is approximately
the same as the
+12 UR, the +24V
Fuse is blown or
the +24V Power
Supply is defective.

28.9V-37.7V
?
From Main Menu, press

28.9V-37.7V

NO

DIAGNOSTICS
MORE
INITIALIZATION

YES

YES
- Verify +24V between Pins 1
and 2 of Shear Valve Driver
Board. If not present re-seat
cable.
- Re-seat cable from FCM-J8
to SVDM-J2.

- Lubricate Shear Valve Driver


(VP-42).

YES

- Replace Shear Valve Driver


Assy. (PL-9.1).

Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

- Check Fuse again.

- Verify proper operation.

- Replace cable. (PL-1).


Problem
Corrected

NO

Fuse
Blown
Total Call

- Replace FCM (PL-14.1).

YES

?
NO

- Replace Power Supply


(PL-1).

Part Number 9140300 August 1998

2 - 70

5#7URXEOHVKRRWLQJ IP-20 Shear Valve Position Fault


IP-20 Shear Valve Position Fault (Page 2 of 2)

IP-20 Shear Valve Position Fault, 2 of 2

- If the Fuse continues to blow,


assemblies on the +12V UR or
+24V UR must be
disconnected one at a time
until the shorted assembly is
found and replaced. (See
Figures)
- Power-down instrument and
connect Meter between TP2+12V or TP1-+24V and TP4 on
PSM and remove connectors
until short is found.
- Replace defective assembly and
verify proper operation.

To
C D M 2-J3
FC M -J5

To
S D M /C D M -J3

J1
UR RET
1
3
4 +1 2 V U R
5 +2 4 V U R
J2
UR RET
1
3
4 +1 2 V U R
+2 4 V U R
5

B a ck

C D M 2 & S DM /C D M P o w er C on n ecto rs
J1
J3
+2 4 V U R
5
1
Fro m
+1 2 V U R
To
2
P.S . 4
SDM A
UR RET
J1/J2 3
3
1

PSM

J2
1
2
3

J3
To
S h ea r
Valve
D rive r-J3

1
2

+2 4 V U R
RET

N o te: J4 and J5 a re no t o n
S D M /C D M

To
S D M 9 -J1 0

J4
+2 4 V U R
1
+1 2 V U R
2
3 UR RET

J4
1
2

To
SDM C

3
FC M
J5
1
2
3 2 0 11

CELL-DYN 3200 SYSTEM SERVICE MANUAL

To
SDM B

Part Number 9140300 August 1998

To
SDM D

2 - 71

5#7URXEOHVKRRWLQJ IP-21 Flags


IP-21 Flags (Page 1 of 2)

Start

Problem
Corrected

- Ensure Customer has verified


an Instrument problem and
not a Specimen Specific
problem with multiple Manual
Differential Counts.

YES

?
Total Call
NO

- Clean Fan Filters and verify


all Fans are running properly.
- Ensure there is at least 6" of
clearance behind and on the
left side of Instrument.

- Perform Autoclean

- Ensure Instrument is not


subject to drastic temperature
variations (direct sunlight,
heating/air conditioning
ducts).

MORE

- Initialize and Prime


Instrument
- If Fan Filters were clogged
and then cleaned, allow a 15
minute temperature
stabilization period.
- Replace RBC Diluent (RBC/
PLT Flags) or WBC Lyse
(WBC Flags) Container.

- Verify Background
Counts (VP-56).

From Main Menu, press


SPECIAL PROTOCOLS

YES

AUTOCLEAN
- Clean Shear Valve (VP-48).
- Manually clean, debubble, and wet
Optical Flow Cell (VP-44), (VP45).

NO

Backgrounds
OK

- Run a Normal Patient


Sample exhibiting flags 5
times and print the
results.
- Observe the results and
refer the the System
Flags Table.

- Clean Optics Bench (VP-43).

- Replace In-line Sheath Filter (PL-4.2).


NO
Go to (IP-8).

YES

Problem
Corrected
?

Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 72

5#7URXEOHVKRRWLQJ IP-21 Flags


IP-21 Flags (Page 2 of 2)

Flagging
Parameters

WBC Flags Only

All Flagging

RBC and/or PLT Flags Only

- Verify Pressure 2 (VP-16) and


proper Sheath flow to Optical
Flow Cell. Troubleshoot as
necessary.

- From Main Menu, press

- From Main Menu, press

DIAGNOSTICS

DIAGNOSTICS

F12

F12

- Replace Optical Flow Cell


(PL-10).

F1

F1

- Replace MAM (PL-12).

SET POINT ENTRY

SET POINT ENTRY

- Replace SPM (PL-10).

- Verify WBC Threshold is 360.

- Verify RBC/PLT Threshold is 550.

- Replace CPU/DCM (PL-13).

- Ensure WBC Mixing Chamber is


being rinsed and drained properly.

- Ensure RBC/PLT Mixing Chamber is


being rinsed and drained properly.

- Verify/adjust WBC Gain (VP-23).

- Verify 0 o and 10 o Log Amplifiers (VP-28).

- Perform WBC OPTI-CAL


Verification/Adjustment (VP-24).

- Verify 0 o and 10 o Gain (VP-26), (VP-27).


- Replace MAM (PL-12).

Var Lym

LRI

- Replace MAM (PL-12).

- Replace SPM (PL-10).

Band

URI

- Replace SPM (PL-10).

- Replace CPU/DCM (PL-13).

IG

LURI

- Replace CPU/DCM (PL-13).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

System Flags Table


WBC Flags PLT Flags

RBC Flags
RBC Morph

Blast

Part Number 9140300 August 1998

2 - 73

5#7URXEOHVKRRWLQJ IP-22 External Waste Full


IP-22 External Waste Full (Page 1 of 2)

Start

System Uses
Waste Sensor Cable

NO

- Ensure Dummy Plug is


installed
- Reseat Dummy Plug
3 2 01 2

Problem
Corrected

- Empty Waste Container

YES

Total Call

- Reseat Waste Sensor Plug


- Clear Fault

NO
- Insert Insulated Tool into
Sensor Jack to open
contacts. See Figure

NO
Problem
Corrected
?

Total Call

YES

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 74

5#7URXEOHVKRRWLQJ IP-22 External Waste Full


IP-22 External Waste Full (Page 2 of 2)

Problem
Corrected

YES

NO

- Disconnect Sensor Cable from Waste


Container and Sensor Jack and verify
infinite resistance between Fluid
Contacts. Replace as necessary (PL-3.1).

Problem
Corrected

YES

Total Call

Perform in the following order until problem is


corrected:
- Reseat CDM2, J7 and FCM, J2
- Replace Waste Sensor Jack (PL-3.2)
- Replace CDM2 (PL-16)
- Replace FCM (PL-14.1)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

NO

Part Number 9140300 August 1998

2 - 75

5#7URXEOHVKRRWLQJ IP-23 Blank Display (Page 1 of 1)


IP-23 Blank Display (Page 1 of 1)
Start
- Connect Monitor to
Laptop. See Figure

- Turn Brightness and Contrast


controls fully clockwise.

- Open Computer Door to


observe 7-segment LED on
CPU/DCM.

- Verify Laptop
information displays on
Monitor.

- Verify Monitor Power and Power


Cord.
- With Power off, check Video
Cable, re-seat all Computer
Connectors, re-seat VGA Board.

- Cycle Analyzer Power and


verify the following:
- Stepper Motors all home.

NO

- Valves activate.
- 7-segment LED increments
until "E" and "1" are alternately
displayed.

Information
Displays

YES

- Analyzer "beeps" after


Initialization.

Problem
Corrected

YES

Total Call
YES
Data Station CRT

- Replace VGA Board (PL17.2).

NO

Initialization
OK
?
- Replace Monitor (PL17.3).

NO
- Troubleshoot
"dead" Analyzer
problem.

Attach Video Cable


From CRT to Laptop
External Monitor Port
Laptop
Computer

CELL-DYN 3200 SYSTEM SERVICE MANUAL

32101

Part Number 9140300 August 1998

2 - 76

5#7URXEOHVKRRWLQJ IP-24 HSSL Failures/Message Reception Time-Out (Page 1 of 1)


IP-24 HSSL Failures/Message Reception Time-Out (Page 1 of 1)

Start
- In order replace the
following.

- With Power OFF, check


and re-seat connectors on
the CPU/DCM, and Main
Computer.

- HSSL Cable (PL-17.3)


- CPU/DCM (PL-13)
- Serial Link/Keyboard
Controller (PL-17.2)

- Re-seat the HSSL Board.


- Check and re-connect the
HSSL Cable.

NO

- Reload HSSL Com (VP-67)

Problem
Corrected
?
YES
Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 77

5#7URXEOHVKRRWLQJ IP-25 Hard Drive Errors (Page 1 of 1)


IP-25 Hard Drive Errors (Page 1 of 1)

Start
- Format the Hard Drive (VP-61).
- Install Software (VP-53).
- Load System Setup (VP-65), (VP63).

- With Power OFF, re-seat


cable between Hard Drive
and Motherboard.
- Re-seat Power Cable on
Hard Drive and CDM.

NO

Problem
Corrected

NO

?
Problem
Corrected

YES

?
Total Call

- Replace Hard Drive (PL-17.1).

YES
Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 78

5#7URXEOHVKRRWLQJ IP-26 Printer Errors


IP-26 Printer Errors (Page 1 of 2)

Start

- Check power and power


cable.

- Ensure Printer Power is


ON, and Printer is Online.
- Ensure paper is loaded
properly.

Is Printer
Working

- Press On-line Button.


YES

NO

?
Clear Printer buffer.

NO

- From Main Menu, press

YES
Printer
On-Line
Printer
On-Line

NO

YES

- Replace Printer.

SETUP
CUSTOMIZE
REPORT

Total Call

STOP PRINTING
CONFIRM STOP

Clear Printer buffer.


- From Main Menu, press
SETUP
CUSTOMIZE
REPORT

Printer
Reset &
Print
?

NO

STOP PRINTING
CONFIRM STOP

YES
Total Call

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 79

5#7URXEOHVKRRWLQJ IP-26 Printer Errors


IP-26 Printer Errors (Page 2 of 2)

1
- Connect Laptop Printer to
Printer Connector to
verify problem.

- Try printing with alternate


Printer Cable.

- Replace I/O Board (PL17.2).

NO
NO

Prints
OK

- Replace Motherboard
(PL-17.2).

Laptop
Printer
Prints
?

?
YES
YES
- Replace Printer.
- Replace Printer Cable
(PL-17.3).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2 - 80

5#7URXEOHVKRRWLQJ IP-27 Sample Loader & Aspiration Tower Error Messages


IP-27 Sample Loader & Aspiration Tower Error Messages
Error Message

Associated VP(s)

Associated Components

Parts List(s)

Mix Head stuck in angle position

VP-36

Stepper Motor, Home Sensor, SHM 2

PL-18.2, PL-18.1

Mix Head stuck in vertical position

VP-36

Stepper Motor, Home Sensor, SHM 2

PL-18.2, PL-18.1

VP-35

Up/Down Air Cylinder, Sensor, Valve 3,


SHM 2

PL-18.1, PL-18.2,
PL-18.3

VP-35

Up/Down Air Cylinder, Sensor, Valve 3,


SHM 2

PL-18.1, PL-18.2,
PL-18.3

Load zone empty detector malfunction

VP-.39

Sensor, SHM 2

PL-18.2, PL-18.3

Unload zone full detector malfunction

VP-39

Sensor, SHM 2

PL-18.2, PL-18.3

Unload zone nearly full detector


malfunction

VP-39

Mix Head not at top position


Mix Head stuck at top position

Tube stuck in position 3


Probe stuck at home position
Probe unable to reach home position
Invalid tube height
Unload area hardware malfunction
Tube dropped during mixing

PL-18.2, PL-18.3

VP-38

Tube Sensor Board, Rack Advance


Cylinder, Valve 1, SHM 2

PL-18.2, PL-18.3

VP-31

Aspiration Tower, Home Sensor, Needle


SHM 1

PL-6.1, PL-8

VP-31

Aspiration Tower, Home Sensor, Needle


SHM 1

PL-6.1, PL-8

VP-34

Aspiration Tower, Sensors S1/S2, SHM 1

PL-6.1, PL-8

Unload Side Sensors, SHM 2

PL-18.2, PL-18.3

Mixer Head, Valve 4, Regulator, SHM 2

PL-18.1, PL-18.2,
PL-18.3

Rack Index Cylinder, Valve 1, SHM 2

PL-18.1, PL-18.3

VP-38, VP-39
VP-37

Mix Zone Rack position error

VP-38

Unexpected tube in position 4 after


Rack advance

VP-38

Tube lost moving from position 3 to


position 4

VP-38

Rack barcode read failure

VP-40

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Sensor, SHM 2

Tube Sensor Board, SHM 2


Tube Sensor Board, SHM 2

PL-18.1, PL-18.2
PL-18.1, PL-18.2

Rack Barcode Label


Part Number 9140300 August 1998

2 - 81

5#7URXEOHVKRRWLQJ IP-27 Sample Loader & Aspiration Tower Error Messages


IP-27 Sample Loader & Aspiration Tower Error Messages
Error Message
Tube position barcode read failure
Barcode reader failure

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Associated VP(s)
VP-40
VP-33, VP-11

Associated Components

Parts List(s)

Rack Position Barcode Labels


Barcode Reader, Aspiration Tower, SHM 2

Part Number 9140300 August 1998

PL-18.2, PL-6.1, PL-18.1

2 - 82

$SSHQGL[#5$#0#&DEOH#&RQQHFWLRQ#'LDJUDP Table of Contents

$SSHQGL[#5$#0#&DEOH#&RQQHFWLRQ#'LDJUDP
Table of Contents
Figure 2A-1

Cable Connection Diagram, SL . . . . . . . . . . . . 2

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2A - 1

$SSHQGL[#5$#0#&DEOH#&RQQHFWLRQ#'LDJUDP
Figure 2A-1 Cable Connection Diagram, SL
9601320
FCM

SOL
1
2
3
4
5

(SOL 11-18)
SDM 1
SOL
9600930
NC
J1
J6
7
J2
J7
J3
J8
8
J4
J9
9520830
J5
J10

1
2
3
4
5

9520830

J56
J57
J58

J3
J1

SPEAKER &
STATUS BD

J2

SP

P/O
SDM/CDM
(SOL 41)
J61
J68
J67

J2

3
4

J3
J4

J15
J17

J5

J12

12"VAC OVFL
9520528
8"VAC OVFL
9520833
TEST CONN.
9520879

PRES PUMP
VAC PUMP

1
2
3
4
5

SDM 5
9600930
J6
J1
6
J2
J7
7
J3
J8
NC
J4
J9
9520859
J5
J10

9520860
NC
NC

9520851

J23
J24

9520861
9520866

9520849
PERIPHERAL BUS

J2
J5
J11

J10

(SOL 61-68)

1
2
3
4
5

SDM 6
9600930
J6
J1
6
J2
J7
7
J3
J8
8
J4
J9
J5
J10 9320860

1
2
3
4
5

J18

J8

9520385

HGB

J2
J3

NC

LASER

9520869

LASER P/S
9520896

12V, 24V UNREG.

J4

J15

NC
NC

J5

J3
9520853
J10

NC
NC

J7

J6
J12

J6
J7

9520001
FAN

J3

J8

9520620
FAN

J4

J13

9520829

J8
J1
J7

J13

J14

9520002
PWR SW

J9

J4

9520861
9520897
9520898

J6

9520789

J6
J7

J2

9520782

9520865
9520543

9520845
9520844

J2

MOTOR
A CORE SYR.

J1

J2

MOTOR
E PROBE

J1

J2

MOTOR
G SAMPLE PUMP

J1

9520155
9520842

J2
J10

NC

9520894

J3

SENSOR & CHOPPER DRIVER


3X 9600420

J12

NC

J13

NC
NC

J2

J13

J5

J14
9520892

1
SOLENOID

CHAIN CABLE

CHAIN CABLE

FLOW PANEL (X2)


PCS PANEL
LASER PANEL

J1

J2

MOTOR
C WBC LYSE SYR.

J1

J2

MOTOR
D RBC/HGB DIL SYR.

CHOPPER DRIVER
3X 9600420

J1

TO SL VAL-1

J2

TO SL VAL-2

J3

TO SL VAL-3

J4

TO SL VAL-4

J5

TO SL VAL-5

J10

TO SL MIXER SENS.

J12
9520883
9520882
9520780

TO TOWER MOTOR

J13

TO TOWER DC-MOTOR

J15

TO COVER SENSOR

J9
J11

J16
ASPIRATION
TOWER
CONTROLER

Y-VALVE ASSY
BOTTOM VIEW

MOTOR
B HGB LYSE SYR.

9601910
SHM(2)

J12

TO PC MOTHERBOARD

J2

NC

9601910
SHM(1)
9520884
J1
TO TOWER SOL.
9520872 TO TOWER SENS.
J10

9520894

J1

9520154

9520563

J14

9520895

9520612

PMT (CH4)

MPM SERIAL LINK

9520847

9520895

SDM1-4.....FLOW PANEL
SDM5,6.....REAGENT PANEL

J1
J2
J3
PMT PREAMP
9601010

9520856

J9

J11

+/-15V

32054

*S/L=SERIAL LINK

9520855

J8

NC

J9
9520871

9601000
PREAMP 10 DEG (CH2)
PMT PREAMP
9601010
J1
PMT (CH3)
J2
J3

J11

J12

J1

9520543

9601000
PREAMP 0 DEG (CH1)

J1

J10

J13

MOTOR

J4
J5

J3

J4

J1

MPM
9600920

J501

9520896
SPARE
9520782

9520854

J11

NC

J2
J1

J5

J7
J4

9520506

MAM
9601950
J2

BACK
9520869
9520866
9520828

12V, 24V UNREG.

J6
J3

NC

ADC2

J3

9520866

9520871

MUX DAC CTRL.

J1

J1

J5

9520841

TO REAGENT
RESERVOIRS

MDM 9601940
J2

9520789

9520841

TOP

9212064

FLOPPY

9520795

9520848

NC

9340008
LINEAR P/S

9212057

NC

HARD DISK

9520796

9520846

J5
J7
J6
J2

J1

PDM
9601980
(SOL 91-98)
SDM 9
9600930
J1
J6
J2
J7
J3
J8
J4
J9
J5
J10

NC
9520893

J4
J1

9520865

9520878

DIL SENS
J8
NC 9520835
J9
SHEATH SENS
J10
9520836
J3

J9

SPM
9601830
9520865
J3

9520892

DILUENT
9520839
P OVER PRESS
SENSOR

SHEAR VALVE DR.


9600900

EX WASTE
9520857

ADC3

J4

9520878

CDM 2
9601080
J7
J4

DAC3

J13

J16

DATA STATION *S/L

9520850

J17

J3
9520841

J19

9520864
9520865
9520500

J3
9520846
J11
9520505

J10
J12

J1
J2

J6 J18

J1
J2 PUMP
J4
J3 RELAY
9601200

J8
J7

NC

J20

J5

9520862

J1

DAC1
ADC1

9520852

ULTRA SONIC
SAMPLE DET

J4

J11
J16

J6

J14

J21

9520831
9520832

J10

9520870

J11

9520829

9520831
J13
9520832
J14

J16

J5
9600910 IN-LINE
REAGENT
SENSOR BD

J14

J1
9601161
2nd SAMPLE DET.
9520841
9520849

9520841
9520849
9520829

J6

J15

9520862

9520826
9520859

J22

J2

9520820

J1

9520879
(SOL 51-58)

J7

J25

VAC/PRES
9601220

START SW

J13

J26
9520838

9520834
1

J1
J12

9520826
NC
SOLENOID

6
7

9520828

J9

CPU
9601820

J6

9520825
NC

9520825

9520847
9520783

+5V. +/-15V
EXT. CPU BUS

2
3

J51
J52
J53
J54

J6

P/O
SDM/CDM

(SOL 31-38)

J3

NC
J9

9520858

(SOL 21-28)
SDM 2
9600930
J1
J6
6
J2
J7
7
J3
J8
8
J4
J9
9520858
J5
J10

9520837

SDM DATA, +5V

9520893

9520890
9520888
9520887
9212106

TO SL MIXER MOTOR
TO BAR CODE READER
TO SL RACK SENSORS
TO SL TUBE SENS ASSY

J16
SAMPLE LOADER
CONTROLLER

4PLCS
9520255 ASY3A

CONNECT THE DOOR AND PLATE TO CHASSIS GND.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2A - 2

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Table of Contents

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV
Table of Contents
Figure 2B-1
Figure 2B-2
Figure 2B-3
Figure 2B-4
Figure 2B-5
Figure 2B-6
Figure 2B-7
Figure 2B-8
Figure 2B-9
Figure 2B-10
Figure 2B-11
Figure 2B-12
Figure 2B-13
Figure 2B-14
Figure 2B-15
Figure 2B-16
Figure 2B-17
Figure 2B-18
Figure 2B-19
Figure 2B-20

ADC Block Diagram . . . . . . . . . . . . . . . . . . . . . 2


ADC Block Diagram . . . . . . . . . . . . . . . . . . . . . 3
DAC Block Diagram . . . . . . . . . . . . . . . . . . . . . 4
Measurement & Subsystem Block Diagram . . 5
Solenoid Driver Modules 1, 2 & SDM/CDM . . . 6
Solenoid Driver Modules 5, 6, 9. . . . . . . . . . . . 7
Motor Driver Block Diagram. . . . . . . . . . . . . . . 8
CS Sensor Interface Block Diagram . . . . . . . . 9
S/L Sensor Interface Block Diagram . . . . . . . 10
Vacuum/Pressure Block Diagram . . . . . . . . . 11
Power Distribution Block Diagram . . . . . . . . . 12
Computer Block Diagram. . . . . . . . . . . . . . . . 13
Measurement Block Diagram. . . . . . . . . . . . . 14
Solenoid Control Block Diagram . . . . . . . . . . 15
Motor Control Block Diagram. . . . . . . . . . . . . 16
Sensor Block Diagram . . . . . . . . . . . . . . . . . . 17
Power Distribution Block Diagram . . . . . . . . . 18
Normal 10, 90 and 0, 90D Scattergrams . . . . 19
Normal 10, 90 and 0, 90D Scattergrams . . . . 20
Normal PLT and RBC Histograms . . . . . . . . . 21

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2B - 1

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-1 ADC Block Diagram


Figure 2B-1 Figure 2B-1ADC Block Diagram

Display

PC Module
VPM
High Speed
Serial Link
Tag Bus
SPM
High
Speed
External
CPU
Bus

FCM
MAM

CPU/DCM

MUX DAC Control Bus


MPM
Serial
Bus
MPM
Peripheral Bus
Serial RS-485 Bus To SHM1
(Aspiration Tower Control)

32043

Serial RS-485 Bus To SHM2


(Sample Loader Control)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2B - 2

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-2 ADC Block Diagram


Figure 2B-2 ADC Block Diagram

Display

PC Module

High Speed
Serial Link

ADC1

A/D

MUX

ADC3
ADC2
Tag Bus

CPU/DCM
ADC4
MAM

SPM

FCM

MUX DAC Control

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

VPM

32044

2B - 3

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-3 DAC Block Diagram


Figure 2B-3 DAC Block Diagram

Display

PC Module
High Speed
Serial Link

DAC1
D/A

DAC2
DAC3

CPU/DCM
DAC4
MAM

SPM

FCM

MUX DAC Control

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

VPM

32045

2B - 4

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-4 Measurement & Subsystem


Figure 2B-4 Measurement & Subsystem Block Diagram

Display

PD

0 o PAM

0 o Lin
10 o Lin

PD

10 o PAM

90 o Lin
90 o D Lin

PMT

90 o PAM

MAM

PC Module

0 o Log
10 o Log

High Speed
Serial Link
SPM
Analog Out

VDYN

Tag Bus
PMT

90 o D PAM
CPU/DCM

VDYN
External CPU Bus

HGB
Flow
Cell

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Peripheral Bus

LED Current
HGB Signal

HGB Voltage
FCM
32046

Part Number 9140300 August 1998

2B - 5

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-5 Solen Dr Mod 1, 2 & SDM/CDM


Figure 2B-5 Solenoid Driver Modules 1, 2 & SDM/CDM
Solenoid Data

Bus
3-1 WC2 Vacuum Supply (5" Hg)
3-2 WC2 Pressure (12 psi)
3-3 RBC Diluent Syringe Supply
3-4 CS Probe Diluent Flush (NC)
CLK

3-6 CS Vent Trap Drain (WC3)


SDM/CDM

3-7 CS Probe Drain (WC4)

(CDM1)

3-8 CS Vent Trap Vent


4-1 NOC Sample Staging
4-7 ON/OFF
4-8 CW/CCW

Motor
Drive
Module

DC

Y-Valve
Motor

1-1 Shear Valve Diluent

Flush

1-2 Sample Aspiration Vacuum


FCM

1-3 Shear Valve Drain (WC4)


CLK

1-4 Open Sample Probe Drain (WC4)


S D M

1-5 Open Sample Probe Diluent Flush (NC)


1-6 Spare
1-7 WC4 Vacuum Supply (12" Hg)
1-8 WC4 Pressure Supply (12 psi)

Peripheral Bus

2-1 RBC Mixing Chamber Drain


2-2 Sample Injection Syringe Sheath Supply
CPU/
DCM

2-3 Main WBC Lyse Supply (NC)


2-4 HGB Lyse Syringe Output
SDM 2
CLK

2-5 WBC Lyse Syringe Output


2-6 Diluent Syringe HGB Dilution
2-7 Diluent Syringe RBC Dilution
2-8 Main HGB Lyse Supply (NC)

1 12 psi Accumulator Vent


2 9 psi Pressure Supply
VPM

3 5 psi Pressure Supply


4 12" Hg Accumulator Vent
5 Vacuum #2 Vacuum Supply

32047

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2B - 6

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-6 Solenoid Dr Modules 5, 6, 9


Figure 2B-6 Solenoid Driver Modules 5, 6, 9
Solenoid Data Bus

FCM

CLK

SDM 5

5-1
5-2
5-3
5-4
5-5
5-6
5-7
5-8

HGB Flow Cell Vent


Staging Pump Input
WBC Cup Vent
RBC/PLT Sample Staging
WBC Sample Staging
Optical Flow Cell Output
Optical Flow Cell Drain
Spare

SDM 6

6-1
6-2
6-3
6-4
6-5
6-6
6-7
6-8

Sheath Reservoir Vacuum (12" Hg)


Diluent Reservoir Vacuum (12" Hg)
Diluent Reservoir Pressure (5 psi)
Sheath Reservoir Pressure (9 psi)
Sheath Reservoir Output (NC)
Diluent Reservoir Output (NC)
WC1 Pressure (12 psi)
WC1 Vent

SDM 9

9-1
9-2
9-3
9-4
9-5
9-6
9-7
9-8

Optical Flow Cell Sheath Flow


WBC Cup Drain (WC3)
HGB Flow Cell Drain (WC3)
HGB Flow Cell Diluent Flush
RBC Mixing Chamber Diluent Flush
WBC Mixing Chamber Diluent Flush
WC4 Vacuum (12" Hg)
WC4 Pressure (12 psi)

CDM 2

Peripheral
Bus
CPU/
DCM

CLK

CLK

32048

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2B - 7

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-7 Motor Driver Block Diagram


Figure 2B-7 Motor Driver Block Diagram
Aspiration Probe Up/Down

A
SHM1

DC Barcode Spin
RS-485
Serial
Bus

MPM
Serial
Bus
MPM

CPU/DCM

Driver

Motor

Sample Delivery Syringe

HGB Lyse Syringe

WBC Lyse Syringe

RBC Diluent Syringe

Open Probe Up/Down

Sample Staging Pump

AC Vacuum Pump

Peripheral Bus
VPM

AC Pressure Pump

Limit Switch (CW)


Shear
Valve
Driver

FCM

4-7
SCM/CDM

4-8

DC Shear Valve Motor


Limit Switch (CCW)

ON/OFF
CW/CCW

Motor
Drive
Module

DC

Y-Valve
Motor

32049

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2B - 8

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-8 CS Sensor Interface Block


Figure 2B-8 CS Sensor Interface Block Diagram

Display
Vacuum Accumulator #1 Wet
VPM
Vacuum Accumulator #2 Wet

PC Module
High Speed
Serial Link

Diluent Empty
WBC Lyse Empty
HGB Lyse Empty

CPU/DCM

Peripheral Bus

External Waste Full


CDM2

RS-485
Serial
Bus

Diluent Reservior Full


Sheath Reservoir Full

FCM
Tube Height (S1)
Tube Height (S2)

SHM1

Shear
Valve
Driver

GS1 Home
Door Closed
Sample Cover

Interface
Board

Limit Switch (CCW)


Limit Switch (CW)
Ultrasonic Short Sample

Y-Valve Closed Mode Sensor


Short Sample

Interface
Board

MDM

Y-Valve Open Mode Sensor

32050

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2B - 9

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-9 S/L Sensor Interface Block


Figure 2B-9 S/L Sensor Interface Block Diagram

Third Tube Sensor


Typical Tube Sensor
Fourth Tube Sensor
J11
25 KHZ LED Drive

RS-485 Bus
To CPU/DCM

Power
From PDM

Tube
J16
SHM2
J12

Load Side Empty Sensor

J9

U. S. Fourth Rack Sensor

U. S. Fifth Rack Sensor

Mixer Home Sensor (Up)


J10
Mixer Head Home Sensor

32105

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2B - 10

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-10 Vacuum/Pressure Block


Figure 2B-10 Vacuum/Pressure Block Diagram

Vacuum/Pressure Module

32051

ADC4

Analog Multiplexer
Multiplexer
&
Sample and Hold Circuitry

Threshold Setpoints

DAC4

P1
SPP1
Pres #1
Regulator
12 psi

P2
SPP2

P1 P2 P3 V1 V2

P3

V1

SPP3

Pres #2
Regulator
9 psi

V2

SPV1

Pres #3
Regulator
5 psi

SPV2

Vac #1
Regulator
12" Hg

V3

Vac #2
Regulator
(Variable)

V5

Relay PCB

Relay PCB
V2

Pressure
Pump

Pres #1
Accumulator
12 psi

Pres #2
Accumulator
9 psi

Pres #3
Accumulator
5 psi

Vacuum
Pump

V1

V4

Vent

Vent

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Vac #1
Accumulator
12" Hg

Part Number 9140300 August 1998

Vac #2
Accumulator
5" Hg
(Varies)

2B - 11

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-11 Power Distribution Block


Figure 2B-11 Power Distribution Block Diagram
AC Line
Pow er Sw itch
MAIN POWER SUPPLY

PDM J2
J3

J13

+ 5, GND-PC MB
+ 12, -12, GND, PF-PC MB
+ 5, -15, GND-MAM, SPM, CPU

J4
+ 5, + 24, GND-MDM

+ 5V
J5
Filter
(CORCOM)

J5

+ 12V
-12V
+ 24V
GND

+ 5, + 12, -12, + 24-MPM


J1

J6
J7

Spare
+ 5, + 15, -15, GND-VPM, FCM

J8
Fuse
J4
(Rear)

+ 15V
-15V

J10
J9

GND

AC Voltage
Selector

J11
J12
J13

+ 5. + 12, GND-Hard Drive


+ 5. + 12, GND-Floppy Drive
+ 5, + 15, -15, GND-SHM1
+ 5, + 15, -15, GND-SHM2

Transformer
J4
(Top)

115VA C

LASER P/S

+ 2400VDC

LASER

115VAC, Rear Fan

J7

115VAC, Side Fan

J8

115VAC, PRM

J2
32052

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2B - 12

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-12 Computer Block Diagram


Figure 2B-12 Computer Block Diagram
H ar d D i s k

Flo p p y D is k

D r iv e

D ri v e

C PU / D C M

K e y b o ard

HSSL
M o t h er b o a rd

Pa r a lle l/
S e ria l Po rt s

H SS L
C o n t r o ller
Pa r a lle l/
S e ria l Po rt s

Bu s
V id e o D is p la y
C o n t r o ller

32053

M e m b r a n e K e y b o a rd
Po w er

V id e o D is p la y

S u p p ly

M o d u le

CELL-DYN 3200 SYSTEM SERVICE MANUAL

C RT

Part Number 9140300 August 1998

2B - 13

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-13 Measurement Block Diagram


Figure 2B-13 Measurement Block Diagram

TP1

TP3

J10
6

Amplifiers

CH 1 Lin
CH 2 Lin
CH 3 Lin
CH 4 Lin
CH 1 Log
CH 2 Log

Lin Amplifiers

TP2
0 Photodiode Preamp

SPM

MAM

J1

TP8
2

Log Amplifiers

J15

J6

1
3
5
7
9
11

1
3
5
7
9
11

CH 1 Lin
CH 2 Lin
CH 3 Lin
CH 4 Lin
CH 1 Log
CH 2 Log

J7
Peak
Hold
Circuits

Amplifier

MUX

1
ADC3
2 To CPU/DCM
3

AGND
AGND

TP1
Amplifiers

AGND

J1

TP4

J12
6

Lin Amplifiers

FCM
TP2
10 Photodiode Preamp

TP9
2

J18
HGB Voltage

TP7 TP1

Log Amplifiers

HGB
Flow
Cell

AGND
AGND

LED Current

Amplifier

1
2
3
4
5

J25
MUX

Amplifier

1
ADC1
2 To CPU/DCM
3

AGND
TP2
Amplifiers

J2

TP5

J13
4

AGND

AGND

J24

Lin Amplifiers

1
TP3

PMT

VDYN/-100

AGND

Amplifier
8

ADC4
From
VPM

TP1
90 PMT Preamp
2

32055

AGND

TP2
Amplifiers

TP6

J14
4

Lin Amplifiers

TP3

PMT
VDYN/-100

AGND

J2

TP2

TP1
90 D PMT Preamp
2

+10V Reference

AGND

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2B - 14

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-14 Solenoid Control Block


Figure 2B-14 Solenoid Control Block Diagram

CLK

J1

CLK

CLK

J2

WC4 Vacuum Supply (12" Hg)


WC4 Pressure (12 psi)
RBC Diluent Syringe Supply
CS Probe Diluent Flush (NC)
CS Vent Trap Drain (WC3)
CS Probe Drain (WC4)
CS Vent Trap Vent
NOC Sample Staging

CLK

CLK

Peripheral Bus

J2
J1

ON/OFF
CW/CCW

J6

Y
Valve
Motor

J2

J1
J9 J2
J3
J4
J5
J6
J10 J7
J8

CDM2
J1
J2

1-1
1-2
1-3
1-4
1-5
1-6
1-7
1-8

Shear Valve Diluent Flush


Sample Aspiration Vacuum
Shear Valve Drain (WC4)
Open Sample Probe Drain (WC4)
Open Sample Probe Diluent Flush (NC)
Spare
WC2 Vacuum Supply (4" Hg)
WC2 Pressure Supply (12 psi)

2-1
2-2
2-3
2-4
2-5
2-6
2-7
2-8

RBC Mixing Chamber Drain


Sample Injection Syringe Sheath Supply
Main WBC Lyse Supply (NC)
HGB Lyse Syringe Output
WBC Lyse Syringe Output
Diluent Syringe HGB Dilution
Diluent Syringe RBC Dilution
Main HGB Lyse Supply (NC)

5-1
5-2
5-3
5-4
5-5
5-6
5-7
5-8

HGB Flow Cell Vent


Staging Pump Input
WBC Cup Vent
RBC/PLT Sample Staging
WBC Sample Staging
Optical Flow Cell Output
Optical Flow Cell Drain
Spare

J2
1
2
To
3
5 SDMB
6
7
J4
1
2
3
5
6
7

SDM6
J1
J9 J2
J3
J4
J5
J6
J10 J7
J8

J6

SDM1
SDM2
J1
J9 J2
J3
J4
J5
J6
J10 J7
J8

On CLK A
1
Hi CLK A
2
On CLK B
3
Hi CLK B
From 4
On CLK C
FCM 5
Hi CLK C
6 On CLK D
7
Hi CLK D
8
Reset
9

SDM5
DC

1
2
To
3
5 SDMA
6
7

J6

J1

J1
J9 J2
J3
J4
J5
J6
J10 J7
J8

J16
CPU/DCM
J17 J1

+24V UR
5
From 4 +12V UR
P. S. 3 UR RET
1

MDM
J68
J67

J4
J19

3-1
3-2
3-3
3-4
3-6
3-7
3-8
4-1

J6

FCM

J1

J3
J51
J52
J53
J54
J56
J9 J57
J58
J61

J3

CDM Connectors

SDM/CDM

Solenoid Data/+5V/5V Ret.

6-1
6-2
6-3
6-4
6-5
6-6
6-7
6-8

Sheath Reservoir Vacuum (12" Hg)


Diluent Reservoir Vacuum (12" Hg)
Diluent Reservoir Pressure (5 psi)
Sheath Reservoir Pressure (9 psi)
Sheath Reservoir Output (NC)
Diluent Reservoir Output (NC)
WC1 Pressure (12 psi)
WC1 Vent

9-1
9-2
9-3
9-4
9-5
9-6
9-7
9-8

Optical Flow Cell Sheath Flow


WBC Mixing Chamber Drain (WC3)
HGB Flow Cell Drain (WC3)
HGB Flow Cell Diluent Flush
RBC Mixing Chamber Diluent Flush
WBC Mixing Chamber Diluent Flush
WC3 Vacuum (12" Hg)
WC3 Pressure (12 psi)

To
SDMC

J5
1
2
To
3
5 SDMD
6
7

SDM9
J1
J9 J2
J3
J4
J5
J6
J10 J7
J8

32056

VPM
J17

RS-485 Bus

J1
J2
J3
J4
J5

SHM1

Aspiration Tower

J16 J1
J2
J3
J4
J5
J6
J7
J8

GS2 Stop
Door Lock
Spare
Spare
Spare
Spare
Spare
Spare

RS-485 Bus

J16

J1
J2
J3
J4
J5
J6
J7
J8

J1

SHM Solenoid Connector


+24V
J1-J8

On CLK 1
Hi CLK 1
On CLK 2
Hi CLK 2
On CLK 3
Hi CLK 3
On CLK 4
Hi CLK 4
Reset

J3

1
2
3
4
To
5
CDM1
6
7
8
9
15

PD0
PD1
PD2
PD3
PD4
PD5
PD6
PD7
+5V

1
2
3
4
To
5
SDMs
6
7
8
9
10

SDM Connectors & Driver Outputs

J9
PD0
1
PD1
2
PD2
3
PD3
4
PD4
5
PD5
6
PD6
7
PD7
8
+5V
9
10

+24VUR

Hi/Lo

+12VUR

Driver

TP1

2
AGND
On/Off

SHM2

FCM Connectors

1 12 psi Accumulator Vent


2 9 psi Pressure Supply
3 5 psi Pressure Supply
4 12" Hg Accumulator Vent
5 Vacuum #2 Vacuum Supply

Sample Loader
V1 Rack Advance
V2 Load Side Cross Transfer
V3 Mixer Lift
V4 Mixer Bladders
V5 Unload Side Cross Transfer
Spare
Spare
Spare

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Driver

On CLK 5
Hi CLK 5
On CLK 6
Hi CLK 6
Reset

J4

1
2
3
To
4 CDM2
9
15

+24VUR
+12VUR
UR RET
On CLK 9
Hi CLK 9
Reset

J1-J8

DGND

DGND
J2

1
2
To
3
5 SDM9
6
7

J10
+24VUR
1 +12VUR
2 UR RET
3
On CLK
5 Hi CLK
6
Reset
7

On/Off
TP2

Driver

Solenoid Drive

1
2

AGND

Part Number 9140300 August 1998

2B - 15

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-15 Motor Control Block Diagram


Figure 2B-15 Motor Control Block Diagram
MPM Stepper Motor Control
Sample Loader Motor Control

Aspiration Tower Motor Control


J6

MPM Serial Link

J3

JI

JI

CDB

J2

Delivery Syringe

Mixer Stepper Motor

GS1 Stepper Motor

CPU/DCM
J4

JI

CDB

J2

HGB Lyse Syringe

PH0

J5

JI

CDB

J2

WBC Lyse Syringe

PH0

J6

JI

CDB

J2

RBC/HGB Diluent Syringe

PH0 1
Motor

MPM

JI

J7

CDB

J2

Open Probe

JI

CDB

J2

MPM Motor Control Block Diagram (Motor 0)

Sample Staging Pump

J3
Ramping
A/D
Converter

FB-

+5V

M0 PH0

M0 PH1

M0 PH1

J3

J3
TO
CDB

M0
Current
Control
Latch

DC

Shear Valve Motor Control

3
4
TO
CDB

Barcode Spin Motor

+24V 1
J8
Driver 2

M0 I1

M0 PH0

1
+24V

Digital Control Bus

M0
Phase
Control
Latch

PH1 4
PH1 5

J3-J14
TO
CDB

M0 I0

J1
MPM
Serial
Link

Typical MPM Motor Control Output Connector


FB+

Discharge

SHM2 J13

PH1
PH1

J9

Discharge
Complete

PH0 2

J13
SHM1

J8

Microprocessor
&
Digital Control

Motor

M0 I0
M0 I1

TO
CDB

M1-M11
Control
Latches
J4-J14

FCM

J8

+5V

DGND

4
5

FB+

J2

On/Off

CW/CCW

Shear
Valve
J4
Driver
Module

+ Drive

- Drive

DC

8
FB-

Shear Valve Motor

9
10
Y-Valve Motor Control

CDB Block Diagram


Chopper Driver Board

J1
PH0

J2

6
4
5

TO
MPM

7
2

Stepper
Motor
Phase 0
Driver

I0
I1

MA

PH0 1

MB

PH0 2

Motor

PH1
Stepper
Motor
Phase 1
Driver

+24V

3 +5V

MA
MB

PH1

J67 2

8
9

FB+
FB-

CW/CCW

FB-

DC

2 J1

4
5

Pump Relay Board

GND
Winding
Voltage
Feedback
(Diagnostics)

Y-Valve Motor

1
MDM J14

3+
FB+

2 J2

Vacuum & Pressure Pump Control


PH1

TP1
1

On/Off

J68 2
SDM/CDM

1
VPM

J18 3
4

+5V
Vac. Pump On
Pres. Pump On

4-

AC In 1
K1
AC Out 2

F1

Line

Neutral J4

115VAC In
Vacuum Pump

1
J1

1
J3

AC

4
3+

AC In 1
K2

4-

AC Out 2

32057

1
J2
2

AC
Pressure Pump

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2B - 16

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-16 Sensor Block Diagram


Figure 2B-16 Sensor Block Diagram
Typical FCM & VPM Fluid Sensor Input

Reagent Empty In-Line Sensors


Aspiration Tower Sensors

IFSB

Electrode +
FLUID
LINE
Electrode -

E1

Diluent Empty

E2

WBC Lyse Empty

E4

HGB Lyse Empty

J1

E5

GS1 Home Sensor

+5V
Tube Height Sensor (S2)

E6

J26
E8
GND

Typical SHM Optical Sensor Input


3

Tube Height Sensor (S1)

J9-10

6 J10
9

Cover Sensor

Door Sensor

SHM1

Typical SHM Magnetic Sensor Input


J9

CDM2

Reagent & Waste Level Sensors

1
2
3
4
5
6
7
8
9
10
11
12

In
In
In
In

Sample Loader Sensors


External Waste Full

1 J7

10

10

Diluent Reservoir Level

1 J8

11

11

13

13

J2

J6
Sheath Reservoir Level

Load Side Empty Sensor

1 J10

GND

15

DGND

U. S. Fifth Rack Sensor

6 J9

U. S. Fouth Rack Sensor

Mixer Head Home Sensor

Typical SHM Tube Sensor Input

+5V
J11

SHM2

15

J10
Mixer Lifter Up Sensor

Second Sample Sensor Board


FCM
+5V

Fourth Tube Sensor

Third Tube Sensor

J11

TP4
TP2
1

+5V

1
2 J14

Ref

J1 3

AGND

Vacuum Accumulator Sensors

In
25KHZ LED Drive
In

AGND

Vacuum #1 Wet

1 J1

Vacuum #2 Wet

VPM
1 J2

AGND

25KHZ LED Drive

1
2
3
4
5
6
7
8
9
10
11
12

Y-Valve Sensors
Closed Mode Sensor

2 J4

Open Mode Sensor

2 J5

Ultrasonic Short Sample Sensor Board

MDM
1

+5V

J1 2
3

2 J11
AGND

Typical MDM Optical Sensor Input

+5V
Shear Valve Driver Module

Limit Sensor Baord


2
J1

3
4

+5V
Valve CCW
Valve CW

2
3
4

J1
TP1

3
J2

DGND
6

J4-J5
+5V

1
2
3

DGND

Valve CW

In

J8

Valve CCW

AGND

RBC Overpressure Sensor

NO

+5V
GND

J10

3
32058

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2B - 17

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-17 Power Distribution Block


Figure 2B-17 Power Distribution Block Diagram
Main Power Supply Power Connectors

Power Distribution Module Power Connectors


J1

BACK
J501
PWR Fail

PWR Fail

1
2
-12V 3
+12V 4
+24V 5
GND 1 6
GND 1 7
+5V 8
+5V 9

J8
To
FCM-J21
VPM-J12

1
2
3
4
5
6

+5V
GND 2
TP8

+15V
GND 2
-15V

+15V

TP2
-12V

J9
Input
From
P.S.
J4

1
2
3
4

-15V
GND 2
KEY
+15V

TP7
+24V

PWR Fail
+5V
+12V
-12V
GND 1
GND 1

J10
To
PC
Hard
Disk

+12V
1 GND 2
2 GND 2
3 +5V
4

TP1
TP5

J11
To
PC
Floppy
Disk

1
2
3
4

+12V
GND 2
GND 2
+5V

GND 1

GND 2

GND 1
-15V

+24V
1
2 +5V
3
4 +15V
5 -15V
6 GND 2
7 GND 2
8

To
SHM 2-J12

To
PDM-J1

To
CDM2-J3
FCM-J5

J3
1
2
3
4
5
6

1
2
3
4
5
6
7
8

J5
+24V 1
+5V
2
GND 1 3

To PC
Motherboard

To
MAM-J3
SPM-J3
CPU/DCM-J14

To
SDM/CDM-J3

To
Shear
Valve
Driver-J3

To
PDM-J9

+5V

+24V
+5V
+15V
-15V
GND 2

TP3

J6
-12V
+12V
+24V
GND 1
GND 1
GND 1
+5V

1
2
3
4
5
6
7
8

From
P. S.
J1/J2

-12V
+12V
+24V
GND 1
GND 1
+5V
+5V

J2

J1
UR RET
1
3
4 +12V UR
5 +24V UR

1
To
2 SDMB
3

J2
UR RET
1
3
4 +12V UR
5 +24V UR

J4
1
2
3

J5
J4
1 -15V
2 GND 2
3 +15V

1
To
2 SDMD
3

FCM Power Connectors

To
PRM-J4
To
MPM-J2

To
Laser
Power
Supply

J7
TP4
+12V

Typical MPM Motor Control Output Connector


J3-J14

-12V 1
+12V 2
3
GND 1 4
GND 1 5
GND 1 6
7
+5V 8

J2
115VAC
1
2 Neutral

TOP

J4
115VAC
1 Neutral
2

To
Rear
Fan

J7
115VAC
1
2 Neutral

Spare
To
Side
Fan

J8
115VAC
1 Neutral
2

1
+24V
+5V
M0 I0
M0 I1
M0 PH0
M0 PH1
FB+
FB-

To
SDMC

J3
+24V UR
1
2 RET

-15V

GND 2

CDM 1 & 2 Power Connectors


J1
+24V UR
1
5
To
+12V UR
4
2 SDMA
UR RET
3
3
1

J3

To
MDM-J3

TP6

J13
1
2
3
4
5
6
7
8

To PC
Motherboard

J4
+5V
+5V
GND 1
GND 1

J12

To
SHM 1-J12

J2
GND 1
1
GND 1 2
+5V 3
4
+5V 5
+5V 6

Input
From
P.S.
J501

1
2
3
4
5
6
7
8
9

J3
To
SDMs-J9

To
SDM9-J10
To
Shear
Valve
Driver-J2
To
Ultrasonic
Sample
Sensor-J3
To
Second
Sample
Sensor-J1

9 +5V
10 GND
J4
+24V UR
1
2 +12V UR
3 UR RET
J8
1 +5V
3 GND
J11
1 +5V
3 GND
J14
1 +5V
3 GND

3
4
5
6

TO
CDB

Note: Pin configurations on the assemblies that are not


shown are the same as those shown.

32059

7
8
9
10

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2B - 18

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-18 Normal 10,90 & 0,90D S'grams


Figure 2B-18 Normal 10, 90 and 0, 90D Scattergrams

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2B - 19

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-19 Normal 10,90 & 0,90D S'grams


Figure 2B-19 Normal 10, 90 and 0, 90D Scattergrams
4 th Cut

3 rd Cut

M
E

0 Size

90 Dep

B
M

0
B - Basophil
E - Eosinophil

CELL-DYN 3200 SYSTEM SERVICE MANUAL

10 Complexity
L - Lymphocyte
M - Monocyte

32103

N - Neutrophil

Part Number 9140300 August 1998

2B - 20

$SSHQGL[#5%#0#6\VWHP#%ORFN#'LDJUDPV Figure 2B-20 Norm PLT & RBC Histograms


Figure 2B-20 Normal PLT and RBC Histograms
RBC/PLT

PLT

PLT

1. Curve Starts to the Right of the Lower Threshold.

RBC

2. Single Peak
32104

3. Good Distance From Lower Threshold at Peak

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2B - 21

$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Table of Contents

$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV
Table of Contents
Figure 2C-1 Flow Diagram, Complete . . . . . . . . . . . . . . . . . 2
Figure 2C-2 Sample Loader Flow Diagram . . . . . . . . . . . . . 3
Figure 2C-3 Vacuum/Pressure Flow Diagram . . . . . . . . . . . 4
Figure 2C-4 Reagent Flow Diagram . . . . . . . . . . . . . . . . . . 5
Figure 2C-5 Sample Aspiration Flow Diagram. . . . . . . . . . . 6
Figure 2C-6 Dilution and Mixing Flow Diagram . . . . . . . . . . 7
Figure 2C-7 Sample Staging Flow Diagram . . . . . . . . . . . . 8
Figure 2C-8 Sample Delivery Flow Diagram . . . . . . . . . . . . 9
Figure 2C-9 Waste Flow Diagram . . . . . . . . . . . . . . . . . . . 10
Figure 2C-10 Vent Flow Diagram . . . . . . . . . . . . . . . . . . . . 11
Service Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2C - 1

$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-1 Flow Diagram, Complete


Figure 2C-1

Flow Diagram, Complete


REAGENT & WASTE PANEL

MUFFLER

BLOOD/IN
EMPTY
SENSORS

PRES
PUMP

62

63

61

64

HGB DIL/IN

FILTER

WBC LYSE
FRONT VALVE

DILUENT
RESERVOIR

REAR VALVE

HGB LYSE

SHEATH
RESERVOIR

28

23
PS1

TOP

HGB LYSE (BLU)

SENSOR

RBC DIL/IN, FARSIDE

RBC/OUT

TO AIR

TO AIR

WBC DIL/IN, FARSIDE

HGB/OUT

DILUENT
WBC LYSE
WASTE

TO AIR

BLOOD/OUT, FARSIDE

WBC/OUT

HGB LYSE

ACC 1
PRES 1

FILTER (SMALL)

WBC LYSE (VIO)


66

TO AIR

(12 psi)

DILUENT (RED)

FILTER
(LARGE)

WBC
FLOW
CELL

91

65
96

94

DILUENT (RED)

1 2 3 4 5

56
53

WBC OUT

PS2
ACC 2
PRES 2
(9 psi)

95

RBC
MIXING
CHAMBER

52

HGB
12 uL
RBC
1.67 uL

WBC
M. C.

51

HGB OUT

WBC
20 uL

MOTOR-X
BLOOD
SAMPLE
VALVE

VENT
ACC

22

MOTOR-G
RBC DIL

RBC OUT
11

12

41

MOTOR-A

VENT

Y-VALVE
47,48

TO SAMPLE LOADER
(SL MODEL ONLY)

21

VAC

14
15

PUMP

92

TO AIR

26
25

38
MOTOR-E

24

33
10 mL
RBC
DIL
SYRINGE
SY4

34
WC-4

TO AIR

VENT
TRAP

WC-3

SENSOR
37

ACC 4

DRAIN

31

32

5
PS5

97

CLOSED
MODE
PROBE

98

MOTOR-C

WC-2

2.5 mL
HGB
LYSE
SY2

2.5 mL
WBC
LYSE
SYRINGE
SY3

MOTOR-D

VAC 1
(12" Hg)

68

17
27

OPEN
SAMPLE
PROBE

67

93

PRESSURE
SWITCH

VAC 1 (GRN)

PS4

500 uL
SAMPLE
INJECTION
SY1

TRAP

TRAP

MUFFLER

TRAP

HGB
FLOW
CELL

ULTRASONIC
SENSOR

13

SENSOR

TO AIR

PERISTALTIC
PUMP

55

HGB DIL

PS3
ACC 3
PRES 3
(5 psi)

57

WBC LYSE

SAMPLE
SENSOR 2

SENSOR

54

MOTOR-B

WC-1

18

36

VAC 1
(GRN)

VAC 2 (BRN)

SENSOR

PRESS 1 (ORN)
ACC 5
VAC 2
(VAR.)

2 WAY PINCH VALVE NORMALLY OPEN


WASTE (BLK)
2 WAY PINCH VALVE NORMALLY CLOSED
DRAIN

VAC 2 (BRN)
AIR FLOW RESTRICTOR
32060

CHECK VALVE. ARROW SHOWS


DIRECTION OF FLOW

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2C - 2

$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-2 Sample Loader Flow Diagram


Figure 2C-2

Sample Loader Flow Diagram


12 psi

12" Hg.

5um
Filter

3-Way
Valve
NC

Out

.010" Orifice

Rack Advance

NO
V1
3-Way
Valve
Load Side Cross Transfer
NC

Out
.016" Orifice

NO
V2
3-Way
Valve
NC

Unload Side Cross Transfer

Out
.016" Orifice

NO
V5
3-Way
Valve

Mixer Head Up/Down

To
Air

NC

Out
.016" Orifice

NO
V3
3-Way
Valve
5 psi
NC

Out

Mixer Bladders

NO
V4
Regulator
32061

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2C - 3

$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-3 Vacuum/Pressure Flow Diagram


Figure 2C-3

Vacuum/Pressure Flow Diagram


REAGENT & WASTE PANEL
TO AIR
MUFFLER

WASTE

62

PRES
PUMP

63

61

64

SENSORS
TO AIR

TO AIR

FILTER

DILUENT
RESERVOIR

SHEATH
RESERVOIR

PS1
SENSOR

ACC 1
PRES 1
(12 psi)
12 psi

2 WAY PINCH VALVE NORMALLY OPEN

9 psi

2 WAY PINCH VALVE NORMALLY CLOSED

PS2

5 psi
12" Hg

AIR FLOW RESTRICTOR

VAC 2 (Varies)

SENSOR
CHECK VALVE. ARROW SHOWS
DIRECTION OF FLOW

ACC 2
PRES 2
(9 psi)

3
TRAP

PS3
SENSOR
ACC 3
PRES 3
(5 psi)

TRAP

TRAP

67
17

TO SAMPLE LOADER
(SL MODEL ONLY)

TO AIR
MUFFLER
VAC
PUMP

VAC 1 (GRN)

PS4

WC-2

WC-4

TO AIR

WC-1

WC-3

SENSOR
ACC 4

18
4

VAC 1
(12" Hg)
DRAIN
5
PS5

31

32

97

98

VAC 1
(GRN)

VAC 2 (BRN)

SENSOR

PRESS 1 (ORN)
ACC 5
VAC 2
(VAR.)

32062

DRAIN

CELL-DYN 3200 SYSTEM SERVICE MANUAL

VAC 2 (BRN)

Part Number 9140300 August 1998

2C - 4

$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-4 Reagent Flow Diagram


Figure 2C-4

Reagent Flow Diagram

REAGENT & WASTE PANEL

BLOOD/OUT, FARSIDE

WBC/OUT

HGB LYSE
DILUENT
WBC LYSE

WBC DIL/IN, FARSIDE

HGB/OUT
BLOOD/IN
EMPTY
SENSORS

HGB DIL/IN

RBC DIL/IN, FARSIDE

RBC/OUT

WBC LYSE
FRONT VALVE

DILUENT
RESERVOIR

REAR VALVE

HGB LYSE

SHEATH
RESERVOIR

28

23

TOP

HGB LYSE (BLU)

FILTER (SMALL)

WBC LYSE (VIO)


66

WBC
FLOW
CELL

91

65
DILUENT (RED)
96

94

DILUENT (RED)

12345

WBC LYSE
95

RBC
MIXING
CHAMBER

MOTOR-X
BLOOD
SAMPLE
VALVE

WBC
20 uL

HGB
12 uL
RBC
1.67 uL

WBC
M. C.

HGB OUT
HGB DIL

22
MOTOR-G
RBC DIL

RBC OUT

HGB
FLOW
CELL

11

500 uL
SAMPLE
INJECTION
SY1

DILUENT/SHEATH

MOTOR-A

SHEATH
HGB LYSE
PRESSURE
SWITCH

WBC LYSE

2 WAY PINCH VALVE NORMALLY OPEN

15

27

OPEN
SAMPLE
PROBE

25

10 mL
RBC
DIL
SYRINGE
SY4

34

AIR FLOW RESTRICTOR


CHECK VALVE. ARROW SHOWS
DIRECTION OF FLOW

CELL-DYN 3200 SYSTEM SERVICE MANUAL

24

33

MOTOR-E
2 WAY PINCH VALVE NORMALLY CLOSED

26

2.5 mL
HGB
LYSE
SY2

2.5 mL
WBC
LYSE
SYRINGE
SY3

MOTOR-D

MOTOR-C

32063

MOTOR-B

CLOSED
MODE
PROBE

Part Number 9140300 August 1998

2C - 5

$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-5 Sample Aspiration Flow


Figure 2C-5

Sample Aspiration Flow Diagram


2 WAY PINCH VALVE NORMALLY OPEN

SAMPLE
SENSOR 2

2 WAY PINCH VALVE NORMALLY CLOSED

WBC
20 uL

MOTOR-X
BLOOD
SAMPLE
VALVE

HGB
12 uL
RBC
1.67 uL

AIR FLOW RESTRICTOR


CHECK VALVE. ARROW SHOWS
DIRECTION OF FLOW

ULTRASONIC
SENSOR

12

Y-VALVE
47,48

TO AIR

TRAP

17

OPEN
SAMPLE
PROBE
38
WC-2

MOTOR-E
VENT
TRAP

CLOSED
MODE
32064

PS5
VAC 2 (BRN)

SENSOR

OPEN MODE SAMPLE

ACC 5
VAC 2
(VAR.)

CLOSED MODE SAMPLE


OPEN/CLOSED MODE SAMPLE
VAC 2 (BRN)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

VACUUM #2

Part Number 9140300 August 1998

2C - 6

$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-6 Dilution and Mixing Flow


Figure 2C-6

Dilution and Mixing Flow Diagram

(1) RBC/PLT

WBC
FLOW
CELL

TO AIR
FILTER
(LARGE)

1 2345
54

53

52

(2) WBC
WBC
M. C.

51

(3) NOC
55

VENT
ACC

PERISTALTIC
PUMP
MOTOR-G

RBC
MIXING
CHAMBER

HGB
FLOW
CELL
41
32066

2 WAY PINCH VALVE NORMALLY OPEN

RBC/PLT STAGING
WBC STAGING

2 WAY PINCH VALVE NORMALLY CLOSED


AIR FLOW RESTRICTOR

NOC STAGING
STAGING PUMP LINES

WC-1

MIXING CHAMBER VENTS


CHECK VALVE. ARROW SHOWS
DIRECTION OF FLOW

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2C - 7

$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-7 Sample Staging Flow Diagram


Figure 2C-7

Sample Staging Flow Diagram

(1) RBC/PLT

WBC
FLOW
CELL

TO AIR
FILTER
(LARGE)

1 2345
54

53

52

(2) WBC
WBC
M. C.

51

(3) NOC
55

VENT
ACC

PERISTALTIC
PUMP
MOTOR-G

RBC
MIXING
CHAMBER

HGB
FLOW
CELL
41
32066

2 WAY PINCH VALVE NORMALLY OPEN

RBC/PLT STAGING
WBC STAGING

2 WAY PINCH VALVE NORMALLY CLOSED


AIR FLOW RESTRICTOR

NOC STAGING
STAGING PUMP LINES

WC-1

MIXING CHAMBER VENTS


CHECK VALVE. ARROW SHOWS
DIRECTION OF FLOW

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2C - 8

$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-8 Sample Delivery Flow Diagram


Figure 2C-8

Sample Delivery Flow Diagram

SHEATH
RESERVOIR

TOP

TO AIR

FILTER (SMALL)
WBC
FLOW
CELL

91

65
DILUENT (RED)

FILTER
(LARGE)

12345
56
(1) RBC
(2) WBC
(3) NOC

VENT
ACC

500 uL
SAMPLE
INJECTION
SY1
MOTOR-A

68
2 WAY PINCH VALVE NORMALLY OPEN

SAMPLE DELIVERY
FLOW CELL OUT

2 WAY PINCH VALVE NORMALLY CLOSED

WASTE CHAMBER #1 VENT

AIR FLOW RESTRICTOR

SHEATH FLOW
32067

WC-1

CHECK VALVE. ARROW SHOWS


DIRECTION OF FLOW

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2C - 9

$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-9 Waste Flow Diagram


Figure 2C-9

Waste Flow Diagram


BLOOD/OUT, FARSIDE

WBC/OUT

WBC DIL/IN, FARSIDE

HGB/OUT
BLOOD/IN
WASTE

HGB DIL/IN

RBC DIL/IN, FARSIDE

RBC/OUT
FRONT VALVE

REAR VALVE

WBC
FLOW
CELL
12345
56
57
52
WBC
M. C.

WBC
20 uL HGB
12 uL
RBC
1.67 uL

MOTOR-G

RBC
MIXING
CHAMBER

TRAP

HGB
FLOW
CELL

12

92

13

32068

PERISTALTIC
PUMP

TRAP

TRAP

93

Y-VALVE
47,48
21
OPEN
SAMPLE
PROBE

14

WC-2

MOTOR-E

WC-4

WC-1

VENT
TRAP

WC-3
37

ACC 4
VAC 1
(12" Hg)

CLOSED
MODE
PROBE

DRAIN

36

2 WAY PINCH VALVE NORMALLY OPEN

ACC 5
VAC 2
(VAR.)

WASTE (BLK)

WASTE CHAMBER #1 WASTE


WASTE CHAMBER #2 WASTE

2 WAY PINCH VALVE NORMALLY CLOSED


DRAIN
AIR FLOW RESTRICTOR

WASTE CHAMBER #3 WASTE


WASTE CHAMBER #4 WASTE
MAIN WASTE OUT

CHECK VALVE. ARROW SHOWS


DIRECTION OF FLOW

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

2C - 10

$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Figure 2C-10 Vent Flow Diagram


Figure 2C-10 Vent Flow Diagram
TO AIR

2 WAY PINCH VALVE NORMALLY OPEN

MUFFLER
2 WAY PINCH VALVE NORMALLY CLOSED

PRES
PUMP

AIR FLOW RESTRICTOR

TO AIR

TO AIR

FILTER

CHECK VALVE. ARROW SHOWS


DIRECTION OF FLOW

TO AIR

VENT TO AIR

FILTER
(LARGE)

ACC 1
PRES 1

53

(12 psi)
51

WBC
M. C.

VENT
ACC
RBC
MIXING
CHAMBER

HGB
FLOW
CELL

TO AIR
MUFFLER
68

VAC

TO AIR

PUMP

TO AIR

38

32069

WC-1
ACC 4
VAC 1
(12" Hg)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

4
VENT
TRAP

Part Number 9140300 August 1998

2C - 11

$SSHQGL[#5&#0#6\VWHP#)ORZ#'LDJUDPV Service Commands


Service Commands

DIAGNOSTICS

3200 Hidden Screens

SET POINT ENTRY

From the Menu listed, press the function keys indicated to


access the desired Service Screen.

LATEX/BLOOD COMPARE
TOGGLE CS/SL

Example: To access the Set Point Entry Screen, enter the


DIAGNOSTICS Menu then press F12/F1.
From Main Menu:

Pressing F12/F1 accesses the following


Commands.

DIAG FUNCTIONS
ERROR LOG

F12/F8 to go to C:\CD3200 prompt

STORE RAW ON/OFF


DEBUG ON/OFF

From C:\CD3200
prompt:

Pressing DIAG FUNCTIONS accesses


the following commands.

Type rl to reboot to Initialized


Type rl /nopri to reboot to Ready

PEEK COMMAND

(Use only if Analyzer was in the Ready


Mode before entering DOS.)

POKE COMMAND
MOTOR CONTROL

CALIBRATION

CELL-DYN 3200 SYSTEM SERVICE MANUAL

F12/F1 accesses Dilution Factors

Part Number 9140300 August 1998

2C - 12

6#0#3DUWV#/LVWV #Table of Contents

6#0#3DUWV#/LVWV
Table of Contents
How to Use This Chapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Reagents and Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Alphabetical Parts List Cross Reference . . . . . . . . . . . . . . . . . 4
PL-1 Power Supply Module . . . . . . . . . . . . . . . . . . . . . . 11
PL-2 Power Distribution Module & Laser Supply. . . . . . 13
PL-3 Reagent Lines & Fans . . . . . . . . . . . . . . . . . . . . . 16
PL-4 Flow Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
PL-5 Vacuum/Pressure . . . . . . . . . . . . . . . . . . . . . . . . . 22
PL-6 Aspiration Tower & Open Probe Assemblies). . . . 24
PL-7 Syringe Drivers (AB, CD) . . . . . . . . . . . . . . . . . . . 27
PL-8 Peri-Pump, Sample Handler Module #1,
& Open Probe Assembly. . . . . . . . . . . . . . . . . . . . 29
PL-9 Shear Valve Driver, Motor Driver Module,
& Sample Sensor Boards . . . . . . . . . . . . . . . . . . . 31
PL-10 Laser Bench . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
PL-11 Motor Processor Module. . . . . . . . . . . . . . . . . . . . 36
PL-12 Main Amplifier & Signal Processor Modules . . . . . 38
PL-13 Central Processing Unit/Device Control Module. . 40
PL-14 Flow Control Module, View #1 . . . . . . . . . . . . . . . 42
PL-15 Solenoid Driver Modules 1, 2, & Solenoid
Driver Module/Cable Distribution Module . . . . . . . 45
PL-16 Solenoid Driver Modules 5,6,9 & Cable
Distribution Module #2 . . . . . . . . . . . . . . . . . . . . . 48
PL-17 Main Computer Boards & Computer Assemblies . 51
PL-18 Sample Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
PL-19 Covers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Flow Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Pneumatic Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
CELL-DYN 3200 SYSTEM SERVICE MANUAL

Sample Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Tubing Designator/Catalog Number Table . . . . . . . . . . . . 66

How to Use This Chapter


This chapter contains Parts Identification information for the
CELL-DYN 3200 System. It includes:

Alphabetical Listing of Parts and corresponding PL


numbers;

Illustrated Parts Lists, showing part location, catalog


number and description.

System Tubing Maps with table of tubing designators,


catalog numbers and descriptions.

Table of frequently used supplies and tools.

Part Number 9140300 August 1998

3-1

6#0#3DUWV#/LVWV Table of Contents


To find information about a part:

From the Alphabetical Listing:

Click on the red PL number to jump to the appropriate


Parts Description/Catalog Number table.
From an illustrated Parts List:

Find the part in the drawing, note its item number, and
click anywhere on the drawing to jump to the
appropriate Parts Description table.
From a Tubing Map:

Find the Tubing Designator on the tubing, e.g., small


silicon tubing is designator S1. Click anywhere on the
Tubing Map to jump to the Tubing Designator/Catalog
Number table.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3-2

3 - Parts Lists Reagents and Supplies

Reagents and Supplies


Item

Part Number

Description

99120-01

Calibrator, CELL-DYN 3000

93111-01

Ctrl, CELL-DYN 3000 (2.5 mL)

99129-01

Ctrl, CELL-DYN 3000 (3.0 mL)

03H78-01

Reagent, WBC Lyse (960 mL)

03H79-01

Reagent, DIL/STH (20 L)

03H80-01

Reagent, HGB/NOC (960 mL)

99644-01

Enzymatic Cleaner

1360-02

ISE Conditioning Solutions

8160580701

Versalube

10

8160600301

5.0 Standard Reference Particles

11

8160600401

7.0 Standard Reference Particles

12

8160600501

3.0 Standard Reference Particles

13

06H63-01

Rack, Sample Loader (1 ea)

14

04H87-01

Racks, Sample Loader (5 ea)

15

92532-01

Connector, Ser Loopback

16

06H71-01

Specifications, Interface

17

8938080601

Disks, Installation (1 & 2)

18

8938070001

Disk, Service

19

06H61-01

Disk, Setup

20

06H90-01

Kit, Software Upgrade Dom.

21

06H91-01

Kit, Software Upgrade Int.

22

8945020201

Fixture, Voltage Divider

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3-3

6#0#3DUWV#/LVWV Alphabetical Parts List Cross Reference

Alphabetical Parts List Cross Reference


Description

List #

Description

List #

Bd, Sensor, Sample Tube

PL-18

Bd, SER Link/KBd Intfc

PL-17

Bd, Shear Valve Driver, SVDM

PL-9

Bd, Signal Processor Module, SPM

PL-12

3.0 Standard Reference Particles

Rea/Sup

5.0 Standard Reference Particles

Rea/Sup

7.0 Standard Reference Particles

Rea/Sup

Bd, Solenoid Driver Module, SDM

PL-15 PL-16

Accumulator, Vac/Pres (Lg)

PL-5

Bd, Status Alert

PL-19

Accumulator, Vac/Pres (SM)

PL-5

Bd, Ultrasonic Sensor

PL-9

Barcode Reader

PL-18

Bd, Video, VGA W/1MB RAM

PL-17

Bd, Cable Dist Module, CDM

PL-16

Bd, VPM (Vac/Pres Module)

PL-5

Bd, Chopper Driver Module, CDB

PL-6

Bd. Sample Handler Module, SHM

PL-8

Bd, CPU/DCM

PL-13

Belt, Timing

PL-7

Bd, DC Motor Driver Module

PL-9

Belt, Timing Spinner Assy

PL-6

Bd, Flow Control Module, FCM

PL-14

Belt, Timing, GS1 Assy

PL-6

Bd, HV/Preamp

PL-10

Belt, Timing, GS1 Motor

PL-6

Bd, I/O Serial/Parallel

PL-17

Bench, Optics (Complete)

PL-10

Bd, In-line Sensor

PL-3

Block, Wash, Closed Sample

PL-6

Bd, Main Amplifier Module

PL-12

Block, Wash, Open Sample

PL-6

Bd, w/RAM & CPU Mother

PL-17

Bottle, Vent Accumulator

PL-4

Bd, Motor Processor Module, MPM

PL-11

Bottle, Waste (large)

PL-4

Bd, PDM (Power Dist. Module)

PL-2

Bottle, Waste (small)

PL-4

Bd, Photodiode Preamp

PL-10

Cable Kit, Internal I/O

PL-17

Bd, PRM (Pump Relay Module)

PL-5
PL-18

Cable, 900 PRE-J2 to MAM-J12

PL-10 PL-12

Bd, Sample Handler Module


Bd, Sample Sensor#2

PL-9

Cable, 900 D PRE-J2 to MAM-J14

PL-10 PL-12

Bd, SDM/CDM

PL-15

Cable, CDM-J6 to FCM-J2

PL-16

CELL-DYN 3200 SYSTEM SERVICE MANUAL

PL-7

Part Number 9140300 August 1998

3-4

6#0#3DUWV#/LVWV Alphabetical Parts List Cross Reference


Description

List #

Description

List #

Cable, CDM2-J10 to SH Sensor

PL-16

Cable, FCM-J26 to ILSB-J1

PL-14 PL-3

Cable, CDM2-J4 to VPM-J6

PL-16

Cable, FCM-J4 to SDM9-J10

PL-14

Cable, CDM2-J7 to Ext Waste

PL-3

Cable, FCM-J5 to PSM(B)-J1

PL-14

Cable, CDM2-J8 to DIL Sensor

PL-16

Cable, FCM-J7 to SAB-J1

PL-14

Cable, CPU-J1 to SHM1-J16

PL-13

Cable, FCM-J8 to SVDM-J2

PL-14

Cable, CPU-J10 to SPM-J5

PL-12

Cable, FCM-J9 to Start SW

PL-14

Cable, CPU-J12 to SPM-J7

PL-13

Cable, Floppy Drive INTFC

PL-17

Cable, CPU-J17 to SHM2-J16

PL-13

Cable, Hard Drive INTFC

PL-17

Cable, CPU-J3 to SPM-J4

PL-13

Cable, HGB Flow Cell to FCM

PL-4

Cable, CPU-J6 to MPM-J1

PL-11 PL-13

Cable, HSSL (Internal)

PL-13

Cable, CPU-J7 to FCM-J25

PL-13

Cable, HSSL to Monitor

PL-17

Cable, CPU-J8 to FCM-J22

PL-13

Cable, Interface, HSSL

PL-17

Cable, DCM2-J3 to PSM(B)-J1

PL-16

Cable, MAM-J10 to PD PRE

PL-12

Cable, External CPU Bus

PL-12

Cable, MAM-J12 to PD PRE

PL-12

Cable, FCM-J-8 to HGB F. Cell

PL-14

Cable, MAM-J15 to SPM-J6

PL-12

Cable, FCM-J1 to CDM1-J6

PL-14

Cable, MAM-J3 to PDM-J4

PL-12

Cable, MUX DAC Ctrl Bus

PL-5

Cable, MAM-J4 to CPU-J9

PL-12

Cable, FCM-J10 to DIL OP Sen

PL-14

Cable, MDM-J1 to SDM4-J8

PL-9

Cable, FCM-J18 to HGB Cell

PL-4

Cable, MDM-J2 to SDM4-J7

PL-9

Cable, FCM-J2 to CDM2-J6

PL-14

Cable, MPM-J3 to CDB A-J1

PL-7 PL-11

Cable, FCM-J21 to PDM-J8

PL-14

Cable, MPM-J4 to CDB B-J1

PL-7 PL-11

Cable, FCM-J22 to CPU-J8

PL-14

Cable, MPM-J5 to CDB C-J1

PL-7

Cable, FCM-J23 to VPM-J13

PL-14

Cable, MPM-J6 to CDB D-J1

PL-7 PL-11

Cable, FCM-J24 to VPM-J14

PL-14

Cable, MPM-J7 to CDB E-J1

PL-11

Cable, FCM-J25 to CPU-J7

PL-14

Cable, MPM-J7 to CDM-J1

PL-6

CELL-DYN 3200 SYSTEM SERVICE MANUAL

PL-16

PL-13

PL-13

Part Number 9140300 August 1998

PL-19

PL-13

PL-11

3-5

6#0#3DUWV#/LVWV Alphabetical Parts List Cross Reference


Description

List #

Cable, MPM-J9 to CDB G-J1

PL-8 PL-11

Cable, MUX DAC CTRL Bus

Description

List #

Cable, SDM Data/+5V Bus

PL-12 PL-13
PL-14

PL-14
PL-16

Cable, SDM5-J10 to CDM2-J1

PL-16

Cable, Nose Cover Sensor

PL-8 PL-19

Cable, SDM5-J10 to CDM2-J2

PL-16

Cable, P/O Ultrasonic Sen

PL-14

Cable, SDM9-J10 to FCM-J4

PL-16

Cable, PDM-J10 to Hard Drive

PL-2 PL-17

Cable, Serial Periph Bus

Cable, PDM-J11 to Floppy Drive

PL-2 PL-17

PL-5 PL-13
PL-14

Cable, PDM-J12 to SHM1-J12

PL-2

Cable, SHM1-J1 to GS2 Solenoid

PL-8

Cable, PDM-J13 to SHM2-J12

PL-2

Cable, SHM1-J12 to PDM-J12

PL-8

Cable, PDM-J2/J3 to Mother Bd.

PL-2 PL-17

Cable, SHM1-J13 to GS1 Motor

PL-8

Cable, PDM-J4 to MAM-J3

PL-2 PL-13

Cable, SHM1-J16 to CPU-J1

PL-8

Cable, PDM-J5 to MDM-J3

PL-2 PL-9

Cable, SHM1-J8 to Spin Motor

PL-8

Cable, PDM-J6 to MPM-J2

PL-2 PL-11

Cable, SHM1-J9 to Sensors

PL-8

Cable, PDM-J8 to FCM-J21

PL-2 PL-5

Cable, SHM2-J10 to MIX Sens

PL-18

Cable, PMT Socket

PL-10

Cable, SHM2-J11 to Tube Sens

PL-18

Cable, Printer, Parallel

PL-17

Cable, SHM2-J12 to PDM-J13

PL-18

Cable, PSM (B)-J1 to CDM2-J3

PL-1

Cable, SHM2-J13 to MIX MTR

PL-18

Cable, PSM (B)-J3 to SVD-J3

PL-1

Cable, SHM2-J15 to BCD RDR

PL-18

Cable, PSM (B)-J4 to PDM-J9

PL-1 PL-2

Cable, SHM2-J16 to CPU-J17

PL-18

Cable, PSM (Back)-J2 to CDM1-J3

PL-1 PL-15

Cable, SHM2-J9 to RACK Sens

PL-18

Cable, PSM (T)-J13 to PWR SW

PL-1

Cable, SPM-J4 to CPU-J3

PL-12

Cable, PSM (T)-J2 to PRM-J4

PL-1

Cable, SS2-J1 to FCM-J14

PL-9 PL-14

Cable, PSM (T)-J501 to PDM-J1

PL-1 PL-2

Cable, SVDM J3 to PSM (B)-J3

PL-9

Cable, PSM (T)-J7 to XXXFAN

PL-1

Cable, SVDM J4 to DC Motor

PL-9

Cable, PSM (T)-J8 to XXXFAN

PL-1

Cable, SVDM-J1 to Sensors

PL-9

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

PL-15

3-6

6#0#3DUWV#/LVWV Alphabetical Parts List Cross Reference


Description

List #

Description

List #

Cable, SVDM-J2 to FCM-J8

PL-9

Cover, Right Side

PL-19

Cable, Vac1 Wet Sensor

PL-5

Cover, SL Bottom

PL-19

Cable, Vac2 Wet Sensor

PL-5

Cover, SL Left Side Front

PL-19

Cable, VPM-J13 to FCM-J23

PL-5

Cover, SL Right Side Front

PL-19

Cable, VPM-J14 to FCM-J24

PL-5

Cover, SL Top Skirt

PL-19

Cable, VPM-J18 to PRM-J1

PL-5

Cover, Top

PL-19

Cable, VPM-J6 to CDM2-J4

PL-5

Cylinder, Air, Mixer Lift

PL-18

Cable, Waste Full Sensor

PL-3

Cylinder, Air, X & Y Index

PL-18

Cable, Y-Valve Sensor

PL-8 PL-9

Disk Drive, Floppy, 1.44MB

PL-17

Calibrator, CELL-DYN 3000

Rea/Sup

Disk Drive, Hard, 540MB

PL-17

Cap Assy, Diluent/Sheath

PL-3

Disk, Service

Rea/Sup

Cap Assy, HGB/NOC Lyse

PL-3

Disk, Setup

Rea/Sup

Cap Assy, WBC, Lyse

PL-3

Disks, Installation (1 & 2)

Rea/Sup

Cbl, SDM/CDM-J1 to SDM1-J10

PL-15

Driver Assy, Y-Valve, Complete

PL-8

Cbl, SDM/CDM-J2 to SDM2-J10

PL-15

Enzymatic Cleaner

Rea/Sup

Chamber, Mixing, RBC/WBC

PL-4

Fan Guards & Filter, 3 Pcs

PL-3

Clamp, Tubing 1/4 O.D.

TM

Fan, 115VAC, 4.69 SQ

PL-3

Computer Assy, CD3200

PL-17

Filter, 5 Micron, In-line

TM

Connector, Ser Loopback

Rea/Sup

Filter, In-line, Sheath

PL-4

Cord, Power 125Vac 10A

PL-1

Filter, Pressure Pump

PL-5

Cover, Front Nose Section

PL-19

Filter, Vent Accumulator

PL-4

Cover, Keyboard

PL-17

Fitting, Luer Lock

PL-7

Cover, Left Front Door

PL-19

Fitting, Reagent Inlet

PL-3

Cover, Left Side

PL-19

Fitting, Waste Outlet

PL-3

Cover, Right Front Door

PL-19

Fixture, Voltage Divider

Rea/Sup

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3-7

6#0#3DUWV#/LVWV Alphabetical Parts List Cross Reference


Description

Description

List #

Flow Cell Assy, HGB

PL-4

Flow Cell, Optical

PL-10

Fitting (T4)

TM

Fitting 0.062 X 0.062 X 0.062

List #

Keyboard, PCAT

PL-17

Kit, Hardware, 3200

PL-4
PL-19

TM

Kit, Reagent Line (Complete)

PL-3

Fitting Barb Conn 0.062" X 0.062"

TM

Kit, Software Upgrade Dom.

Rea/Sup

Fitting Barb RDCR 0.093" X 0.062"

TM

Kit, Software Upgrade Int.

Rea/Sup

Fitting Barb RDCR 0.125" X 0.062"

TM

Knob, Sample Valve

PL-9

Fitting Barb RDCR 0.125" X 0.093"

TM

Laser, HeNe, 5mw

PL-10

Fitting Conn 0.093 X 0.093

TM

Mirror Perforated

PL-10

Fitting Elb 0.062" X 0.062" X 0.062"

TM

Mixer Head, Complete

PL-18

Fitting Elb 0.093 X 0.093

TM

Monitor, 14 VGA Color

PL-17

Fitting, L, 1/8 in x 1/8 in

TM

Motor, Spinner Assy

PL-6

Fitting, Reducer, 3/16 in to 1/8 in

TM

Motor, Stepper

Fitting RSTR .010 X 1/16

TM

PL-6
PL- 8

Fitting RSTR .016 X 1/16

TM

Motor, Stepper, GS1

PL-6

Fitting T 0.062" X 0.062" X 0.062"

TM

Motor, Stepper, Mixer

PL-18

Fitting T 0.062" X 0.125" X 0.062"

TM

Motor, Y-Valve

PL-8

Fitting T 0.093 X 0.093 X 0.093

TM

Needle, Closed Sample

PL-6

Fitting T 0.125" X 0.125" X 0.125"

TM

Plug

TM

Fuse, 4A 220/240 (200VAC In)

PL-1

Power Supply Laser

PL-2

Fuse, 5A 220/240 Vac

PL-5

Power Supply, AC/DC Main

PL-1

Fuse, 8A 220/240 (<200VAC In)

PL-1

Probe Driver Assy, Open

PL-6

Fuse, Fast 1A 250V 3 AG

PL-2

Probe, Open Sample

PL-6

Fuse, SB 15A 220/240VAC

PL-1

Pump Assy, Peristaltic

PL-8

ISE Cleaning Solutions

Rea/Sup

Pump Head, Vac/Pres

PL-5

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

PL-5

PL-7

3-8

6#0#3DUWV#/LVWV Alphabetical Parts List Cross Reference


Description

List #

Description

List #

Pump Roller Assy

PL-8

Switch, Reed, w/Cable

PL-18

Pump, Pressure (Complete)

PL-5

Switch, Touch Bar

PL-19

Pump, Vacuum (Complete)

PL-5

Syringe Driver Assy, (AB)

PL-7

Rack, Sample Loader (1 ea)

Rea/Sup

Syringe, Diluent (10 mL)

PL-7

Racks, Sample Loader (5 ea)

Rea/Sup

Syringe, Driver Assy, (CD)

PL-7

Reagent, DIL/STH (20 L)

Rea/Sup

Syringe, Sample Inject, 500 uL

PL-7

Reagent, HGB/NOC (960 mL)

Rea/Sup

Syringe, WBC/HGB (2.5 mL)

PL-7

Reagent, WBC Lyse (960 mL)

Rea/Sup

Tubing Silicon (S2)

TM

Regulator, Pressure

PL-18

Tubing Silicon (SY)

TM

Reservoir, Diluent/Sheath

PL-4

Tubing Silicon 1/32 ID x 5/32 OD

TM

Sample Loader, Complete

PL-18

Tubing Tyg 0.062" ID 0.125" OD Pur

TM

Sensor, Overpressure

PL-4

Tubing Tyg 0.062" ID 0.125" OD Red

TM

Sensor, Photodiode & LED

PL-6 PL-8
PL-18 PL-19

Tubing Tyg 0.062

TM

Tubing Tyg 0.125 ID 0.250 OD Grn

TM

Sensor, Sample Detector

PL-9

Tubing Tyg 0.125 ID 0.250 OD Yel

TM

Sensor, Sample, Ultrasonic

PL-8

Tubing Tyg 0.125" ID 0.250" 0D Pur

TM

Shear Valve Driver Assy

PL-9

Tubing Tyg 0.125" ID 0.250" OD Blk

TM

Shield, SHM #1, 2

PL-18

Tubing Tyg 0.125" ID 0.250" OD Grn

TM

Smpl Valve Ceramic, Center

PL-9

Tubing Tyg 0.125" ID 0.250" OD Or

TM

Smpl Valve Ceramic, Front

PL-9

Tubing Tyg 0.125" ID 0.250" OD Red

TM

Smpl Valve Ceramic, Rear

PL-9

Tubing Tyg 0.125 ID 0.250 OD Blu

TM

Solenoid, GS2 Stop

PL-6

Tubing Tyg 1/32 ID 3/32 OD

TM

Speaker Assy

PL-17

Tubing Tyg 3/16 ID 5/16 OD Clr

TM

Specifications, Interface

Rea/Sup

Tubing Tyg 3/32

TM

Switch, AC Input, 16A

PL-17

Tubing Tyg O.125 ID 0.250 OD Gry

TM

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3-9

6#0#3DUWV#/LVWV #Alphabetical Parts List Cross Reference


Description

List #

Tubing Tygon, 1/16 ID

TM

Tubing Tygon, 1/8 ID

TM

Tower Assy, Aspiration

PL-6

Trap Assy, Waste Bottle

PL-4

Tube Assy, Waste Outlet

PL-3

Tube, Photomult, 900

PL-10

Tube, Photomult, 900 DEP

PL-10

Tubing, Peristaltic Pump

PL-8

Valve Assy, Solenoid, 3.9KG

PL-4

Valve Assy, Solenoid, 3.9KG

PL-5

Valve Assy, Solenoid, 5Kg

PL-5

Valve Assy, Solenoid, NC

PL-4

Valve, Check, Duckbill

TM

Valve, Rotary, 3-Port

PL-8

Valve, Solenoid, 3-Way

PL-18

Versalube

Rea/Sup

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 10

6#0#3DUWV#/LVWV #PL-1 Power Supply Module (1 of 2)

PL-1 Power Supply Module (1 of 2)


8
7

9
10
11

6
13/14

5
4

3
2

CELL-DYN 3200 SYSTEM SERVICE MANUAL

12

Part Number 9140300 August 1998

3 - 11

6#0#3DUWV#/LVWV #PL-1 Power Supply Module (2 of 2)

PL-1 Power Supply Module (2 of 2)


Item

Part Number

Description

1
2
3
4
5
6
7
8
9
10
11
12
13

8934000801
8951078201
8952086901
8952082801
8952086601
8510016601
8952087801
8952062001
8952000101
8952000201
8952089601
8240051601
50065-01

Power Supply, AC/DC Main


Cable, PSM (B)-J4 to PDM-J9
Cable, PSM (B)-J3 to SVD-J3
Cable, PSM (Back)-J2 to CDM1-J3
Cable, PSM (B)-J1 to CDM2-J3
Fuse, SB 15A 220/240VAC
Cable, PSM (T)-J2 to PRM-J4
Cable, PSM (T)-J8 to XXXFAN
Cable, PSM (T)-J7 to XXXFAN
Cable, PSM (T)-J13 to PWR SW
Cable, PSM (T)-J501 to PDM-J1
Cord, Power 125Vac 10A
Fuse, 8A 220/240 (<200VAC In)

14

61164-01

Fuse, 4A 220/240 (200VAC In)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 12

6#0#3DUWV#/LVWV #PL-2 Power Distribution Module & Laser Supply (1 of 3)

PL-2 Power Distribution Module & Laser Supply (1 of 3)


5

7
8
9
10
11

3
2
1

CELL-DYN 3200 SYSTEM SERVICE MANUAL

12

Part Number 9140300 August 1998

3 - 13

6#0#3DUWV#/LVWV #PL-2 Power Distribution Module & Laser Supply (2 of 3)

PL-2 Power Distribution Module & Laser Supply (2 of 3)

13

14

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 14

6#0#3DUWV#/LVWV #PL-2 Power Distribution Module & Laser Supply (3 of 3)

PL-2 Power Distribution Module & Laser Supply (3 of 3)


Item
1
2
3
4
5
6
7
8
9
10
11
12
13
14

Part Number
8952078201
8952082901
8952054301
8952089601
8952089701
8952089801
8952061201
8952086501
8952078901
8952089401
8952089501
8960198001
8510019201
8921207601

Description
Cable, PSM (B)-J4 to PDM-J9
Cable, PDM-J8 to FCM-J21
Cable, PDM-J6 to MPM-J2
Cable, PSM (T)-J501 to PDM-J1
Cable, PDM-J10 to Hard Drive
Cable, PDM-J11 to Floppy Drive
Cable, PDM-J3 to Mother Bd.
Cable, PDM-J4 to MAM-J3
Cable, PDM-J5 to MDM-J3
Cable, PDM-J12 to SHM1-J12
Cable, PDM-J13 to SHM2-J12
Bd, PDM (Power Dist. Module)
Fuse, Fast 1A 250V 3 AG
Power Supply Laser

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 15

6#0#3DUWV#/LVWV #PL-3 Reagent Lines & Fans (1 of 3)

PL-3 Reagent Lines & Fans (1 of 3)

4
3
6

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 16

6#0#3DUWV#/LVWV #PL-3 Reagent Lines & Fans (2 of 3)

PL-3 Reagent Lines & Fans (2 of 3)


10

11

13

32017

CELL-DYN 3200 SYSTEM SERVICE MANUAL

12

Part Number 9140300 August 1998

3 - 17

6#0#3DUWV#/LVWV #PL-3 Reagent Lines & Fans (3 of 3)

PL-3 Reagent Lines & Fans (3 of 3)


Item
1
2
3
4
5
6
7
8
9
10
11
12
13

Part Number
06H65-01
8921210101
8921205901
8921211501
92161-02
8925089901
8960091001
8952087001
03484-01
8260402701
8952085701
8930038301
8930067601

Description
Kit, Reagent Line (Complete)
Cap Assy, WBC, Lyse
Cap Assy, Diluent/Sheath
Cap Assy, HGB/NOC Lyse
Tube Assy, Waste Outlet
Cable, Waste Full Sensor
Bd, In-line Sensor
Cable, FCM-J26 to ILSB-J1
Fan Guards & Filter, 3 Pcs
Fan, 115VAC, 4.69 SQ
Cable, CDM2-J7 to Ext Waste
Fitting, Reagent Inlet
Fitting, Waste Outlet

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-4 Flow Panel (1 of 3)

PL-4 Flow Panel (1 of 3)


3
1
1
2
3
2

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-4 Flow Panel (2 of 3)

PL-4 Flow Panel (2 of 3)


7
9

8
14

6
5

4
13

15
11
9

CELL-DYN 3200 SYSTEM SERVICE MANUAL

10

12

32019

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-4 Flow Panel (3 of 3)

PL-4 Flow Panel (3 of 3)


Item
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15

Part Number
8921122501
8921016301
8913007601
8921174702
8270721901
8921176502
8921174902
8921206702
8921150901
8510615001
8921174802
8270721901
8921210801
8952077801
8921217201

Description
Valve Assy, Solenoid, 3.9KG
Valve Assy, Solenoid, NC
Kit, Hardware, 3200
Bottle, Waste (small)
Filter, Vent Accumulator
Bottle, Vent Accumulator
Reservoir, Diluent/Sheath
Flow Cell Assy, HGB
Trap Assy, Waste Bottle
Sensor, Overpressure
Bottle, Waste (large)
Filter, In-line, Sheath
Chamber, Mixing, RBC/WBC
Cable, HGB Flow Cell to FCM
Bottle, Waste (small w/tubing)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-5 Vacuum/Pressure (1 of 2)

PL-5 Vacuum/Pressure (1 of 2)

10
11
13
8

12

22

V1

3
3

V4
V2
V3
V5

4
6

15
16

1
5

14

17

2
19
1

18

21
20

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL 5 Vacuum/Pressure (2 of 2)

PL 5 Vacuum/Pressure (2 of 2)
Item
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22

Part #
8921122001
8913007601
8921173201
8921122101
8921153401
8921153001
8410301801
60160-01
8960120001
8952083401
8921173401
8952087501
8952052801
8952082901
8952083101
8952083201
8952084101
8952084901
8952087901
8952083401
8960122001
8270040301

Description
Valve Assy, Solenoid, 5Kg
Kit, Hardware, 3200
Accumulator, Vac/Pres (SM)
Valve Assy, Solenoid, 3.9KG
Pump, Vacuum (Complete)
Pump, Pressure (Complete)
Pump Head, Vac
Fuse, 5A 220/240 Vac
Bd, PRM (Pump Relay Module)
Cable, VPM-J18 to PRM-J1
Accumulator, Vac/Pres (Lg)
Cable, Vac2 Wet Sensor
Cable, Vac1 Wet Sensor
Cable, PDM-J8 to FCM-J21/VPM-J12
Cable, VPM-J13 to FCM-J23
Cable, VPM-J14 to FCM-J24
Cable, MUX DAC CTRL Bus
Cable, Serial Periph Bus
Cable, VPM-J6 to CDM2-J4
Cable, VPM-J18 to PRM-J1
Bd, VPM (Vac/Pres Module)
Filter, Pressure Pump

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-6 Aspiration Tower & Open Probe Assemblies (1 of 3)

PL-6 Aspiration Tower & Open Probe Assemblies (1 of 3)


1

7
2

10

5
8

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-6 Aspiration Tower & Open Probe Assemblies (2 of 3)

PL-6 Aspiration Tower & Open Probe Assemblies (2 of 3)


16

11

12

15

13

14

CELL-DYN 3200 SYSTEM SERVICE MANUAL

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6#0#3DUWV#/LVWV #PL-6 Aspiration Tower & Open Probe Assemblies (3 of 3)

PL-6 Aspiration Tower & Open Probe Assemblies (3 of 3)


Item

Part #

Description

8921208502

Tower Assy, Aspiration

8370830101

Sensor, Photodiode & LED

8250426601

Belt, Timing, GS1 Assy

8250426501

Belt, Timing, GS1 Motor

8921209202

Motor, Spinner Assy

8250426801

Belt, Timing Spinner Assy

03H99-01

Needle, Closed Sample

8933040501

Block, Wash, Closed Smple

8934005801

Solenoid, GS2 Stop

10

8350602701

Motor, Stepper, GS1

11

8921204601

Probe Driver Assy, Open

12

9960042001

Bd, Chopper Driver Module, CDB

13

8952084201

Cable, MPM-J7 to CDM-J1

14

8921076101

Motor, Stepper

15

8931201401

Block, Wash, Open Sample

16

8930094601

Probe, Open Sample

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-7 Syringe Drivers (AB, CD) (1 of 2)

PL-7 Syringe Drivers (AB, CD) (1 of 2)

8
5
5

4
4
7

10
9

6
12

13
9

1
2

CELL-DYN 3200 SYSTEM SERVICE MANUAL

6
5

11

Part Number 9140300 August 1998

6
5

3 - 27

6#0#3DUWV#/LVWV #PL-7 Syringe Drivers (AB, CD) (2 of 2)

PL-7 Syringe Drivers (AB, CD) (2 of 2)


Item

Part #

Description

8921217301

Syringe Driver Assy, (AB)

28561-01

Syringe, WBC/HGB (2.5 mL)

28560-01

Syringe, Sample Inj (500 uL)

04H02-01

Fitting, Luer Lock

8250425701

Belt, Timing

8921076101

Motor, Stepper

8952084401

Cable, MPM-J4 to CDB-J1

8952015401

Cable, MPM-J6 to CDB D-J1

8960042001

Bd, Chopper Driver Module, CDB

10

8952015501

Cable, MPM-J5 to CDB C-J1

11

8921217401

Syringe, Driver Assy, (CD)

12

02H82-01

Syringe, Diluent (10 mL)

13

8952084501

Cable, MPM-J3 to CDB A-J1

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (1 of 2)

PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (1 of 2)
11

12
13

10
9

14

8
7

6
15

1
2
16
16
3
5
CELL-DYN 3200 SYSTEM SERVICE MANUAL

17

Part Number 9140300 August 1998

19

18

17

3 - 29

6#0#3DUWV#/LVWV #PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (2 of 2)

PL-8 Peri-Pump, Sample Handler Module #1, & Open Probe Assembly (2 of 2)
Item

Part #

Description

8921137801

Pump Assy, Peristaltic

8952056301

Cable, MPM-J9 to CDB G-J1

8921076101

Motor, Stepper

91485-01

Tubing, Peristaltic Pump

8921071001

Pump Roller Assy

8960191001

Bd. Sample Handler Module, SHM

8952089201

Cable, SHM1-J16 to CPU-J1

8952088301

Cable, SHM1-J13 to GS1 MOT

8952089401

Cable, SHM1-J12 to PDM-J12

10

8952088401

Cable, SHM1-J1 to GS2 SOL

11

8952088201

Cable, SHM1-J8 to Spin Motor

12

8952078001

Cable, Nose Cover Sensor

13

8952087201

Cable, SHM1-J9 to Sensors

14

8921206101

Driver Assy, Y-Valve, Complete

15

8921207701

Motor, Y-Valve

16

8370830101

Sensor, Photodiode & LED

17

8952087101

Cable, Y-Valve Sensor

18

8250900301

Valve, Rotary, 3-Port

19

8370181801

Sensor, Sample, Ultrasonic

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-9 Shear Valve Driver, Motor Driver Mod, & Sample Sensor Bds (1 of 3)

PL-9 Shear Valve Driver, Motor Driver Module, & Sample Sensor Boards (1 of 3)
8
9

10

11
12

1
4
7
5
6

CELL-DYN 3200 SYSTEM SERVICE MANUAL

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6#0#3DUWV#/LVWV #PL-9 Shear Valve Driver, Motor Driver Mod, & Sample Sensor Bds (2 of 3)

PL-9 Shear Valve Driver, Motor Driver Module, & Sample Sensor Boards (2 of 3)
18

19
P/O Bd.
20
13

17
16

15

CELL-DYN 3200 SYSTEM SERVICE MANUAL

14

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-9 Shear Valve Driver, Motor Driver Mod, & Sample Sensor Bds (3 of 3)

PL-9 Shear Valve Driver, Motor Driver Module, & Sample Sensor Boards (3 of 3)
Item

Part #

Description

8921204901

Shear Valve Driver Assy

8960116701

Sensor, Sample Detector

N/A

Smpl Valve Ceramics, 3 PCS

8932102801

Smpl Valve Ceramic, Rear

06H64-01

Smpl Valve Ceramic, Center

8932103101

Smpl Valve Ceramic, Front

8921167901

Knob, Sample Valve

8960222001

Bd, Shear Valve Driver, SVDM

8952086201

Cable, SVDM-J2 to FCM-J8

10

8952078501

Cable, SVDM-J1 to Sensors

11

8952086901

Cable, SVDM J3 to PSM (B)-J3

12

8952087401

Cable, SVDM J4 to DC Motor

13

8960194001

Bd, DC Motor Driver Module

14

8952078901

Cable, PDM-J5 to MDM-J3

15

8952079601

Cable, MDM-J2 to SDM4-J7

16

8952079501

Cable, MDM-J1 to SDM4-J8

17

8952087101

Cable, Y-Valve Sensor

18

8370181801

Bd, Ultrasonic Sensor

19

8960116701

Bd, Sample Sensor#2

20

8952082001

Cable, SS2-J1 to FCM-J14

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-10 Laser Bench (1 of 2)

PL-10 Laser Bench (1 of 2)


3

4
5

1
10
6
5

8
7

11

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-10 Laser Bench (2 of 2)

PL-10 Laser Bench (2 of 2)


Item

Part #

Description

8921206401

Bench, Optics (Complete)

8010131201

Tube, Photomult, 900

8921137501

Laser, HeNe, 5mw

8570000101

Tube, Photomult, 900 DEP

8960100001

Bd, Photodiode Preamp

8960101001

Bd, HV/Preamp

8921142901

Cable, PMT Socket

8952085501

Cable, 900 PRE-J2 to MAM-J12

8952085601

Cable, 900D PRE-J2 to MAM-J14

10

8921145201

Flow Cell, Optical

11

8921181501

Mirror Perforated

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 35

6#0#3DUWV#/LVWV #PL-11 Motor Processor Module (1 of 2)

PL-11 Motor Processor Module (1 of 2)


2
1
3
4

7
8
9

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-11 Motor Processor Module (2 of 2))

PL-11 Motor Processor Module (2 of 2))


Item

Part #

Description

8960092001

Bd, Motor Processor, MPM

8952084701

Cable, CPU-J6 to MPM-J1

8952054301

Cable, PDM-J6 to MPM-J2

8952084501

Cable, MPM-J3 to CDB A-J1

8952084401

Cable, MPM-J4 to CDB B-J1

8952015501

Cable, MPM-J5 to CDB C-J1

8952015401

Cable, MPM-J6 to CDB D-J1

8952084201

Cable, MPM-J7 to CDB E-J1

8952056301

Cable, MPM-J9 to CDB G-J1

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 37

6#0#3DUWV#/LVWV #PL-12 Main Amplifier & Signal Processor Modules (1 of 2)

PL-12 Main Amplifier & Signal Processor Modules (1 of 2)


1

2
3

13
8
12
11

10

6
9
8
7
6
CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-12 Main Amplifier & Signal Processor Modules (2 of 2)

PL-12 Main Amplifier & Signal Processor Modules (2 of 2)


Item

Part #

Description

8960195001

Bd, Main Amplifier, MAM

8952085501

Cable, 900 PRE-J2 to MAM-J12

8952085601

Cable, 900 D PRE-J2 to MAM-J14

8960183001

Bd, Signal Processor, SPM

8952086501

Cable, MAM-J3 to PDM-J4

8952084801

Cable, MAM-J15 to SPM-J6

8952050001

Cable, SPM-J4 to CPU-J3

8952084101

Cable, MUX DAC CTRL Bus

8952050501

Cable, CPU-J10 to SPM-J5

10

8952084601

Cable, External CPU Bus

11

8952085401

Cable, MAM-J12 to PD PRE

12

8952085301

Cable, MAM-J10 to PD PRE

13

8952050601

Cable, MAM-J4 to CPU-J9

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 39

6#0#3DUWV#/LVWV #PL-13 Central Processing Unit/Device Control Module (1 of 2)

PL-13 Central Processing Unit/Device Control Module (1 of 2)


1

6
4

3
5

11
12
13
15

10

14

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-13 Central Processing Unit/Device Control Module (2 of 2)

PL-13 Central Processing Unit/Device Control Module (2 of 2)


Item

Part #

Description

8960182001

Bd, CPU/DCM

8952084901

Cable, Serial Periph Bus

8952086401

Cable, HSSL (Internal)

8952084701

Cable, CPU-J6 to MPM-J1

8952084601

Cable, External CPU Bus

8952089301

Cable, CPU-J17 to SHM2-J16

8952089201

Cable, CPU-J1 to SHM1-J16

8952084101

Cable, MUX DAC CTRL Bus

8952050001

Cable, CPU-J3 to SPM-J4

10

8952086501

Cable, PDM-J4 to MAM-J3

11

8952085001

Cable, CPU-J12 to SPM-J7

12

8952050601

Cable, MAM-J4 to CPU-J9

13

8952085201

Cable, CPU-J7 to FCM-J25

14

8952050501

Cable, CPU-J10 to SPM-J5

15

8952085101

Cable, CPU-J8 to FCM-J22

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-14 Flow Control Module, View #1 (1 of 3)

PL-14 Flow Control Module, View #1 (1 of 3)


1

10

8
7

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 42

6#0#3DUWV#/LVWV #PL-14 Flow Control Module, View #1 (2 of 3)

PL-14 Flow Control Module, View #1 (2 of 3)

21

20
19
18

16
17

15
14
13

CELL-DYN 3200 SYSTEM SERVICE MANUAL

12

11

Part Number 9140300 August 1998

3 - 43

6#0#3DUWV#/LVWV #PL-14 Flow Control Module, View #1 (3 of 3)

PL-14 Flow Control Module, View #1 (3 of 3)


Item

Part #

Description

8960132001

Bd, Flow Control Module, FCM

8952084101

Cable, MUX DAC CTRL Bus

8952084901

Cable, Serial Periph Bus

8952082501

Cable, FCM-J1 to CDM1-J6

8952082601

Cable, FCM-J2 to CDM2-J6

8952087001

Cable, FCM-J26 to ILSB-J1

8952083701

Cable, SDM Data/+5V Bus

8952086101

Cable, FCM-J4 to SDM9-J10

8952086601

Cable, FCM-J5 to PSM(B)-J1

10

8952083801

Cable, FCM-J7 to SAB-J1

11

8952082901

Cable, FCM-J21 to PDM-J8

12

8952085101

Cable, FCM-J22 to CPU-J8

13

8952083101

Cable, FCM-J23 to VPM-J13

14

8952083201

Cable, FCM-J24 to VPM-J14

15

8952085201

Cable, FCM-J25 to CPU-J7

16

8952038501

Cable, FCM-J-8 to HGB F. Cell

17

8952082001

Cable, SS2-J1 to FCM-J14

18

N/A

Cable, P/O Ultrasonic Sen

19

8952083901

Cable, FCM-J10 to DIL OP Sen

20

8952078301

Cable, FCM-J9 to Start SW

21

8952086201

Cable, FCM-J8 to SVDM-J2

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-15 Solenoid Dr Mod 1, 2, & Solenoid Dr Mod/Cable Distrib Module (1 of 3)

PL-15 Solenoid Driver Modules 1, 2, & Solenoid Driver Module/Cable


Distribution Module (1 of 3)
MDM-J1
MDM-J2

1
6

V41
V38
V32
V36

5
V34

V33

4
V32 V31

3
6
6
3

CELL-DYN 3200 SYSTEM SERVICE MANUAL

5
6

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-15 Solenoid Dr Mod 1, 2, & Solenoid Dr Mod/Cable Distrib Module (2 of 3)

PL-15 Solenoid Driver Modules 1, 2, & Solenoid Driver Module/Cable


Distribution Module (2 of 3)

V18

V21

V17

V22
V23

V15

V24
V25

V14

V13
V11 V12

CELL-DYN 3200 SYSTEM SERVICE MANUAL

V26

Part Number 9140300 August 1998

V27 V28

3 - 46

6#0#3DUWV#/LVWV #PL-15 Solenoid Dr Mod 1, 2, & Solenoid Dr Mod/Cable Distrib Module (3 of 3)

PL-15 Solenoid Driver Modules 1, 2, & Solenoid Driver Module/Cable


Distribution Module (3 of 3)
Item

Part #

Description

8960205001

Bd, SDM/CDM

8952082801

Cable, PSM (Back)-J2 to CDM1-J3

8952083001

Cbl, SDM/CDM-J1 to SDM1-J10

8952085801

Cbl, SDM/CDM-J2 to SDM2-J10

8960093001

Bd, Solenoid Driver Module, SDM

8952083701

Cable, SDM Data/+5V Bus

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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6#0#3DUWV#/LVWV #PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (1 of 3)

PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (1 of 3)


11

12

1
2
1

11
8
10

9
2
8

CELL-DYN 3200 SYSTEM SERVICE MANUAL

6
5

Part Number 9140300 August 1998

3
4

3 - 48

6#0#3DUWV#/LVWV #PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (2 of 3)

PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (2 of 3)


V98
V97
V96
V95

V94
V93
V92 V91

V68
V67

V57
V56

V66

V55

V65

V54

V64

V63
V62

V61

CELL-DYN 3200 SYSTEM SERVICE MANUAL

V53
V52

Part Number 9140300 August 1998

V51

3 - 49

6#0#3DUWV#/LVWV #PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (3 of 3)

PL-16 Solenoid Driver Modules 5,6,9 & Cable Distribution Module #2 (3 of 3)


Item

Part #

Description

8960093001

Bd, Solenoid Driver Module, SDM

8952085901

Cable, SDM5-J10 to CDM2-J1

8960108001

Bd, Cable Dist Module, CDM

8952085701

Cable, CDM2-J7 to EXT Waste

8952083501

Cable, CDM2-J8 to DIL Sensor

8952083601

Cable, CDM2-J10 to SH Sensor

8952082601

Cable, CDM-J6 to FCM-J2

8952086001

Cable, SDM5-J10 to CDM2-J2

8952086601

Cable, DCM2-J3 to PSM(B)-J1

10

8952087901

Cable, CDM2-J4 to VPM-J6

11

8952083701

Cable, SDM Data/+5V Bus

12

8952086101

Cable, SDM9-J10 to FCM-J4

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 50

6#0#3DUWV#/LVWV #PL-17 Main Computer Boards & Computer Assemblies (1 of 4)

PL-17 Main Computer Boards & Computer Assemblies (1 of 4)


1

4
3

5
2
7

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 51

6#0#3DUWV#/LVWV #PL-17 Main Computer Boards & Computer Assemblies (2 of 4)

PL-17 Main Computer Boards & Computer Assemblies (2 of 4)

15

15
14
11
10
16
8
3
9
13

12

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 52

6#0#3DUWV#/LVWV #PL-17 Main Computer Boards & Computer Assemblies (3 of 4)

PL-17 Main Computer Boards & Computer Assemblies (3 of 4)


19

20

18

17
20
22
16
21

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

21

3 - 53

6#0#3DUWV#/LVWV #PL-17 Main Computer Boards & Computer Assemblies (4 of 4)

PL-17 Main Computer Boards & Computer Assemblies (4 of 4)


Item

Part #

Description

8200570201

Disk Drive, Floppy, 1.44MB

8921150601

Speaker Assy

8952056201

Cable, Floppy Drive INTFC

8952089801

Cable, PDM-J11 to Floppy Drive

8200570701

Disk Drive, Hard, 540MB

8952064801

Cable, Hard Drive INTFC

8952089701

Cable, PDM-J10 to Hard Drive

8921205401

Computer Assy, CD3200

8921211901

Bd Mother, w/RAM & CPU

10

8952061201

Cable, PDM-J2/J3 to Mother Bd

11

8170001301

Bd, Video, VGA w/1MB RAM

12

8960109001

Bd, SER Link/KBd INTFC

13

8952082401

Cable, HSSL to Monitor

14

8170000701

Bd, I/O Serial/Parallel

15

8600028501

Cable Kit, Internal I/O

16

06H74-01

Keyboard, PCAT

17

06H75-01

Cover, Keyboard

18

04H44-01

Monitor, 14 VGA Color

19

8510751801

Switch, AC Input, 16A

20

8952087017

Cable, Interface, HSSL

21

20005-01

Cable, Printer, Parallel

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 54

6#0#3DUWV#/LVWV #PL-18 Sample Loader (1 of 5)

PL-18 Sample Loader (1 of 5)


10
32070

7
13
#

11
12

16

15

14
2

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 55

6#0#3DUWV#/LVWV #PL-18 Sample Loader (2 of 5)

PL-18 Sample Loader (2 of 5)

19

18

7
11
17

19
32071

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 56

6#0#3DUWV#/LVWV #PL-18 Sample Loader (3 of 5)

PL-18 Sample Loader (3 of 5)

7
19

7
11

32072

11

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 57

6#0#3DUWV#/LVWV #PL-18 Sample Loader (4 of 5)

PL-18 Sample Loader (4 of 5)

1
4

6
V5
V4

32073

V3
V2
V1

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 58

6#0#3DUWV#/LVWV PL-18 Sample Loader (5 of 5)

PL-18 Sample Loader (5 of 5)


Item

Part #

Description

8960191001

Bd, Sample Handler Module

8952058801

Cable, SHM2-J11 to Tube Sens

8952058601

Cable, SHM2-J15 to BCD RDR

8952089301

Cable, SHM2-J16 to CPU-J17

8952089001

Cable, SHM2-J13 to MIX MTR

8952089501

Cable, SHM2-J12 to PDM-J13

8952088701

Cable, SHM2-J9 to RACK Sens

Part of Sensor

Cable, SHM2-J10 to MIX Sens

8921211601

Sample Loader, Complete

10

8350602501

Motor, Stepper, Mixer

11

8370830101

Sensor, Photodiode & LED

12

8934001701

Barcode Reader

13

8921208301

Mixer Head, Complete

14

8960211001

Bd, Sensor, Sample Tube

15

2880860801

Cylinder, Air, Mixer Lift

16

8510510101

Switch, Reed, w/Cable

17

8450876601

Valve, Solenoid, 3-Way

18

8310842201

Regulator, Pressure

19

8280861301

Cylinder, Air, X & Y Index

20

8932074001

Shield, SHM #1, 2

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 59

6#0#3DUWV#/LVWV #PL-19 Covers (1 of 3)

PL-19 Covers (1 of 3)
6

8
9

6
2

17
8

13
12

8
1

CELL-DYN 3200 SYSTEM SERVICE MANUAL

32074

Part Number 9140300 August 1998

11

10

3 - 60

6#0#3DUWV#/LVWV #PL-19 Covers (2 of 3)

PL-19 Covers (2 of 3)

11

20

19
18
32075

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 61

6#0#3DUWV#/LVWV #PL-19 Covers (3 of 3)

PL-19 Covers (3 of 3)
Item

Part #

Description

8931213001

Cover, SL Bottom

8932063701

Cover, SL Top Skirt

8932073701

Cover, SL Right Side Front

8932073801

Cover, SL Left Side Front

8921217001

Cover, Front Nose Section

8913007601

Kit, Hardware, 3200

8931204801

Cover, Left Side

8913007601

Kit, Hardware, 3200

8931204601

Cover, Top

10

8931204701

Cover, Right Side

11

8932062201

Cover, Right Front Door

12

8370830101

Sensor, Photodiode & LED

13

8952078001

Cable, Nose Cover Sensor

17

8932062101

Cover, Left Front Door

18

8510810901

Switch, Touch Bar

19

8952083801

Cable, FCM-J7 to SAB-J1

20

8960192001

Bd, Status Alert

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 62

6#0#3DUWV#/LVWV #Flow Panel

Flow Panel

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 63

6#0#3DUWV#/LVWV #Pneumatic Unit

Pneumatic Unit

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 64

6#0#3DUWV#/LVWV #Sample Loader

Sample Loader

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 65

6#0#3DUWV#/LVWV #Tubing Designator/Catalog Number Table

Tubing Designator/Catalog Number Table


Item

Part #

Description

A1

8310817501

Fitting RSTR .016 X 1/16

A2

8310817401

Fitting RSTR .010 X 1/16

8310808401

Fitting Conn 0.093 X 0.093

C1

8310802001

Fitting Barb Conn 0.062" X 0.062"

CLP2

8210050201

Clamp, Tubing 1/4 O.D.

CV

8310810201

Valve, Check, Duckbill

EL

8310820001

Fitting Elb 0.093 X 0.093

F1

8270722201

Filter, 5 Micron, In-line

L1

8310807901

Fitting Elb 0.062" X 0.062" X 0.062"

L7

8310805801

Fitting, L, 1/8 in x 1/8 in

P1

03H85-01

Plug

R1

8310805901

Fitting Barb RDCR 0.093" X 0.062"

R2

8310800101

Fitting Barb RDCR 0.125" X 0.062"

R3

8310806201

Fitting Barb RDCR 0.125" X 0.093"

R4

8310807401

Fitting, Reducer, 3/16 in to 1/8 in

S1

9130542

Tubing, Silicon 1/32 ID x 5/32 OD

S2

9130543

Tubing Silicon (S2)

SY

9130544

Tubing Silicon (SY)

T1

8310801001

Fitting T 0.062" X 0.062" X 0.062"

T3

8310800901

Fitting T 0.125" X 0.125" X 0.125"

T4

31082-01

Fitting (T4)

T5

8310809501

Fitting T 0.062" X 0.125" X 0.062"

T8

8310800701

Fitting T 0.093 X 0.093 X 0.093

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 66

6#0#3DUWV#/LVWV #Tubing Designator/Catalog Number Table


Item

Part #

Description

Y1

9130548

Tubing Tygon, 1/16 ID

Y2

9130549

Tubing Tygon, 1/8 ID

Y3

8310654201

Tubing Tygon 3/32

Y4

9130555

Tubing Tygon 1/32 ID 3/32 OD

Y5

8310654001

Tubing Tygon 3/16 ID 5/16 OD Clr

Y622

8310662201

Tubing Tygon 0.062" ID 0.125" OD Red

Y626

8310662601

Tubing Tygon 0.062

Y627

8310662701

Tubing Tygon 0.062" ID 0.125" OD Pur

Y630

8310663001

Tubing Tygon 0.125" ID 0.250" OD Blk

Y631

8310663101

Tubing Tygon 0.125 ID 0.250 OD Grn

Y632

8310663201

Tubing Tygon 0.125" ID 0.250" OD Red

Y633

8310663301

Tubing Tygon 0.125" ID 0.250" OD Or

Y634

8310663401

Tubing Tygon 0.125 ID 0.250 OD Yel

Y635

8310663501

Tubing Tygon 0.125" ID 0.250" OD Grn

Y636

8310663601

Tubing Tygon 0.125 ID 0.250 OD Blu

Y637

8310663701

Tubing Tygon 0.125" ID 0.250" 0D Pur

Y638

8310663801

Tubing Tygon O.125 ID 0.250 OD Gry

Y7

8310653501

Tubing Tygon 3/32

Y FIT

8310810801

Fitting 0.062 X 0.062 X 0.062

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

3 - 67

7#0#5HPRYDO#)#5HSODFHPHQW
Table of Contents

Introduction

Shear Valve & Shear Valve Driver (RR-1). . . . . . . . . . . . . . . . .2


Y-Valve (RR-2) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Ultrasonic Sensor (RR-3) . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
Open Probe Drive Assembly (RR-4) . . . . . . . . . . . . . . . . . . . .22
Optics Bench (RR-5) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
Power Supply (RR-6). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
Vacuum/Pressure Assembly (RR-7) . . . . . . . . . . . . . . . . . . . .39
Tower Assembly (RR-8) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
HGB Flow Cell (RR-9) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52
Sample Loader (RR-10) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57
Syringe Driver Assembly (RR-11) . . . . . . . . . . . . . . . . . . . . . .69

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Step-by-step subassembly removal procedures for field


replaceable components are described on the following illustrated
pages. Most subassemblies are replaced in reverse order of
disassembly, and reassembly is obvious and straightforward.

Part Number 9140300 -- August 1998

4-1

7#5HPRYDO#)#5HSODFHPHQW# Shear Valve & Shear Valve Driver (RR-1)

Shear Valve & Shear Valve


Driver (RR-1)

Figure 4-1:

Front Access Doors

Removal Procedure
1. Ensure that the shear valve is in the
cleaning position using the special
protocols function.
2. With analyzer power off, open the left
and right access doors and remove
the safety cover.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4-2

7#5HPRYDO#)#5HSODFHPHQW# Shear Valve & Shear Valve Driver (RR-1)


3. Remove the shear valve retaining
screw from the center of the shear
valve.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-2:

Shear Valve Retaining Screw

Part Number 9140300 August 1998

4-3

7#5HPRYDO#)#5HSODFHPHQW# Shear Valve & Shear Valve Driver (RR-1)


4. Carefully remove the three ceramic
pieces from the shear valve. Set the
fragile center piece aside. Do not
disconnect tubing from the other two
pieces.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-3:

Shear Valve Ceramics.

Part Number 9140300 August 1998

4-4

7#5HPRYDO#)#5HSODFHPHQW# Shear Valve & Shear Valve Driver (RR-1)


5. Remove the top cover (4 screws) and
the optics cover (two 6-32 screws and
two 8-32 screws.)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-4:

Removing Top Cover and Optics Cover

Part Number 9140300 August 1998

4-5

7#5HPRYDO#)#5HSODFHPHQW# Shear Valve & Shear Valve Driver (RR-1)


6. Remove the 3 screws that hold the
assembly in place.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-5:

Shear Valve Driver MountingScrews

Part Number 9140300 August 1998

4-6

7#5HPRYDO#)#5HSODFHPHQW# Shear Valve & Shear Valve Driver (RR-1)


7. Move the preamplifier cables out of
the way and disconnect cables at J2
and J3 from the Driver Bd.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-6:

Shear Valve Cables J2 and J3.

Part Number 9140300 August 1998

4-7

7#5HPRYDO#)#5HSODFHPHQW# Shear Valve & Shear Valve Driver (RR-1)


8. Angle the assembly and remove from
the back

Figure 4-7:

Shear Valve & Shear Valve Driver Removed.

Replacement Procedure
Replace by following the steps above in
reverse order.

Verification Procedure
See Verification Matrix, Section 5.2.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4-8

7#5HPRYDO#)#5HSODFHPHQW# Y-Valve (RR-2)

Y-Valve (RR-2)

Figure 4-8:

Front Access Doors

Removal Procedure
1. With analyzer power off, open the left
and right access doors and remove
the front cover.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4-9

7#5HPRYDO#)#5HSODFHPHQW# Y-Valve (RR-2)


2. Locate the Y-valve assembly and cut
the tie wrap on the lower left-hand
corner.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-9:

Y-Valve Tie Wrap

Part Number 9140300 August 1998

4 - 10

7#5HPRYDO#)#5HSODFHPHQW# Y-Valve (RR-2)


3. Disconnect the tubing from the three
ports on the valve block

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-10: Y-Valve Block Tubing

Part Number 9140300 August 1998

4 - 11

7#5HPRYDO#)#5HSODFHPHQW# Y-Valve (RR-2)


4. Remove the two screws at the top
near the motor and cut the tie wrap to
the left of the wash block.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-11: Y-Valve Mounting Screws

Part Number 9140300 August 1998

4 - 12

7#5HPRYDO#)#5HSODFHPHQW# Y-Valve (RR-2)


5. Disconnect the two sensor
connections from the base of the
assembly.

Figure 4-12: Y-Valve Sensor Connections.

NOTE: For replacement purposes,


note which socket each comes from
as they are not identified.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 13

7#5HPRYDO#)#5HSODFHPHQW# Y-Valve (RR-2)


6. Remove the top cover (4 screws).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-13: Top Cover Removal

Part Number 9140300 August 1998

4 - 14

7#5HPRYDO#)#5HSODFHPHQW# Y-Valve (RR-2)


7. Cut the two tie wraps from around the
cables above solenoids and the tie
wrap beneath solenoid 13. Disconnect
cable J6 from the Motor Driver Bd.
(located to the left of solenoid 11 in
photo.)

Figure 4-14: Y-Valve Cable J6

Feed cable through front panel and


remove assembly.

Replacement Procedure
Follow the above steps in reverse order.

Verification Procedure
See Verification Matrix, Section 5.2.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 15

7#5HPRYDO#)#5HSODFHPHQW# Ultrasonic Sensor (RR-3)

Ultrasonic Sensor (RR-3)

Figure 4-15: Front Access Doors

Removal Procedure

1. With analyzer power off, open the left


and right access doors and remove
the front cover.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 16

7#5HPRYDO#)#5HSODFHPHQW# Ultrasonic Sensor (RR-3)


2. Remove the top cover (4 screws.)

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-16: Top Cover Removal

Part Number 9140300 August 1998

4 - 17

7#5HPRYDO#)#5HSODFHPHQW# Ultrasonic Sensor (RR-3)


3. Locate the ultrasonic sensor and cut
the tie wrap on the Y-valve

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-17: Y-Valve Tie Wrap

Part Number 9140300 August 1998

4 - 18

7#5HPRYDO#)#5HSODFHPHQW# Ultrasonic Sensor (RR-3)


4. Remove the two fluid connections
from the left and right of the ultrasonic
sensor.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-18: Ultrasonic Sensor Fluid Connections

Part Number 9140300 August 1998

4 - 19

7#5HPRYDO#)#5HSODFHPHQW# Ultrasonic Sensor (RR-3)


5. Remove the two screws on the front
panel which hold the Sensor Bd.
bracket.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-19: Ultrasonic Sensor Holding Screws

Part Number 9140300 August 1998

4 - 20

7#5HPRYDO#)#5HSODFHPHQW# Ultrasonic Sensor (RR-3)


6. Disconnect the gray wires from J1 on
the Sensor Bd and feed connector
through front panel.

Figure 4-20: Ultrasonic Sensor Board Connectors

Replacement Procedure
Follow the above steps in reverse order.

Verification Procedure
See Verification Matrix, Section 5.2.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 21

7#5HPRYDO#)#5HSODFHPHQW# Open Probe Drive Assembly (RR-4)

Open Probe Drive Assembly

Figure 4-21: Front Access Doors

(RR-4)
Removal Procedure
Warning Potential Biohazard:
The aspiration needle/probe is sharp
and potentially contaminated with
infectious materials. Avoid contact
with the tips of the needle/probe.

1. With analyzer power off, open the left


and right access doors and remove
the front cover.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 22

7#5HPRYDO#)#5HSODFHPHQW# Open Probe Drive Assembly (RR-4)


2. Locate and remove the two screws
holding the wash block to the open
probe assembly and let the wash
block dangle.

Figure 4-22: Wash Block Holding Screws

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 23

7#5HPRYDO#)#5HSODFHPHQW# Open Probe Drive Assembly (RR-4)


3. Remove the 4 screws holding the
assembly to the front panel.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-23: Open Probe Assembly Mounting Screws

Part Number 9140300 August 1998

4 - 24

7#5HPRYDO#)#5HSODFHPHQW# Open Probe Drive Assembly (RR-4)


4. Remove the top cover (4 screws).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-24: Top Cover Removal

Part Number 9140300 August 1998

4 - 25

7#5HPRYDO#)#5HSODFHPHQW# Open Probe Drive Assembly (RR-4)


5. Remove the right side cover
(4 screws).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-25: Right Side Cover Removal

Part Number 9140300 August 1998

4 - 26

7#5HPRYDO#)#5HSODFHPHQW# Open Probe Drive Assembly (RR-4)


6. Loosen the upper and lower
thumbscrews and open the CPU door
assembly to gain access to the back of
the aspiration assembly.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-26: CPU Door Access

Part Number 9140300 August 1998

4 - 27

7#5HPRYDO#)#5HSODFHPHQW# Open Probe Drive Assembly (RR-4)


7. Carefully pull aside the white solenoid
wires to remove the drive assembly,
twisting it to the right to remove.
Disconnect the ribbon cable J1 from
the Chopper Driver Bd. and remove
the Probe Assembly.

Figure 4-27: Probe Assembly Removal

Replacement Procedure
Replace by following the above steps in
reverse order.

Verification Procedure
See Verification Matrix, Section 5.2.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 28

7#5HPRYDO#)#5HSODFHPHQW# Optics Bench (RR-5)

Optics Bench (RR-5)

Figure 4-28: Top Cover & Optics Cover Removal

Removal Procedure
'$1*(5=
High Voltage: Assure the
power to the instrument is
off. A high voltage charge
may remain on the laser
connector with the power
off. Do not touch the male
pins. Short male pins to
Chassis.
1. With the analyzer power off, remove
the top cover (4 screws) and the optics
cover (4 screws).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 29

7#5HPRYDO#)#5HSODFHPHQW# Optics Bench (RR-5)


2. Remove the five optics mounting
screws. Remove screws from the
aluminum plate, not the black base of
the Optics Bench.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-29: Optics Bench Mounting Screws

Part Number 9140300 August 1998

4 - 30

7#5HPRYDO#)#5HSODFHPHQW# Optics Bench (RR-5)


3. Remove the 4 signal cables from the
optics bench. Lay the bundle away
from the optics.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-30: Optics Signal Cables

Part Number 9140300 August 1998

4 - 31

7#5HPRYDO#)#5HSODFHPHQW# Optics Bench (RR-5)


4. Remove the two mixing chambers. Do
not disconnect the tubing. Loosen the
two flow cell panel thumbscrews and
open the panel.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-31: Flow Cell Panel

Part Number 9140300 August 1998

4 - 32

7#5HPRYDO#)#5HSODFHPHQW# Optics Bench (RR-5)


5. Disconnect the flow cell tubing (1 - 5)
from the manifold.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-32: Flow Cell Tubing

Part Number 9140300 August 1998

4 - 33

7#5HPRYDO#)#5HSODFHPHQW# Optics Bench (RR-5)


6. Being careful not to stress any wires
that may be connected to the main
power supply, lift the optics bench out
and place on top of back left corner of
the chassis. Disconnect the brown and
yellow wire from top of the main power
supply and remove optics bench.

Figure 4-33: Optics Bench Removal

Replacement Procedure
Replace by following the above steps in
reverse order.

Verification Procedure
See Verification Matrix, Section 5.2.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 34

7#5HPRYDO#)#5HSODFHPHQW# Power Supply (RR-6)

Power Supply (RR-6)

Figure 4-34: Power Supply Connections

Removal Procedure
1. With analyzer power off, remove
optics bench (RR-5).
2. Disconnect the nine connectors
shown on the power supply.

Note 1: Five-pin connections J1, J2,


and J3 are the same.

Note 2: Three-pin connection is


directly above an empty two-pin
connection.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 35

7#5HPRYDO#)#5HSODFHPHQW# Power Supply (RR-6)


3. Remove power supply mounting
screws (6 places).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-35: Power Supply Mounting Screws

Part Number 9140300 August 1998

4 - 36

7#5HPRYDO#)#5HSODFHPHQW# Power Supply (RR-6)


4. Lift power supply up and out.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-36: Power Supply Removal

Part Number 9140300 August 1998

4 - 37

7#5HPRYDO#)#5HSODFHPHQW# Power Supply (RR-6)


Replacement Procedure

Figure 4-37: Power Supply Line Frequency & Line Voltage Select Switches

1. Ensure Line Frequency and Line


Voltage Select switches are set
correctly, according to the photo.
2. Ensure fuse is present and OK.
3. To reinstall, follow the removal steps in
reverse order.

Verification Procedure
See Verification Matrix, Section 5.2.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 38

7#5HPRYDO#)#5HSODFHPHQW# Vacuum/Pressure Assembly (RR-7)

Vacuum/Pressure Assembly
(RR-7)

Figure 4-38: Left Side Panel Removal

Removal Procedure
NOTE: It is no longer necessary to
remove the Vacuum/Pressure
Assembly when servicing the VPM,
pumps, etc. The Assembly is mounted
on movable slides in 3200 analyzers
built after May, 1998. To access the
Assembly:
1. With the analyzer power off, remove
the left side panel (4 screws).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 39

7#5HPRYDO#)#5HSODFHPHQW# Vacuum/Pressure Assembly (RR-7)


2. Disconnect the fan power cord and
remove fan assembly (4 screws).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-39: Fan Assembly Removal

Part Number 9140300 August 1998

4 - 40

7#5HPRYDO#)#5HSODFHPHQW# Vacuum/Pressure Assembly (RR-7)


3. Remove the locking screw and pull
vacuum/pressure assembly out until
unit locks in place.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-40: Vacuum/Pressure Assembly Removal

Part Number 9140300 August 1998

4 - 41

7#5HPRYDO#)#5HSODFHPHQW# Vacuum/Pressure Assembly (RR-7)


Replacement Procedure

Figure 4-41: Vacuum/Pressure Assembly Return

1. To return assembly to normal position,


push up on clip and push assembly
back in place.

Verification Procedure
See Verification Matrix, Section 5.2.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 42

7#5HPRYDO#)#5HSODFHPHQW# Tower Assembly (RR-8)

Tower Assembly (RR-8)

Figure 4-42: Front Access Doors

Removal Procedure
Warning Potential Biohazard:
The aspiration needle/probe is sharp
and potentially contaminated with
infectious materials. Avoid contact
with the tips of the needle/probe.

1. With the analyzer power off, open the


left and right access doors and
remove the safety cover.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 43

7#5HPRYDO#)#5HSODFHPHQW# Tower Assembly (RR-8)


1. Remove the left and right panels from
the rear rail of the sample loader.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-43: Sample Loader Panels

Part Number 9140300 August 1998

4 - 44

7#5HPRYDO#)#5HSODFHPHQW# Tower Assembly (RR-8)


2. Remove the screw at the top of the
tower assembly.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-44: Tower Assembly Screw

Part Number 9140300 August 1998

4 - 45

7#5HPRYDO#)#5HSODFHPHQW# Tower Assembly (RR-8)


3. Loosen the two screws at each end of
the sample loader just one turn.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-45: Sample Loader End Screws

Part Number 9140300 August 1998

4 - 46

7#5HPRYDO#)#5HSODFHPHQW# Tower Assembly (RR-8)


4. Lift sample loader and slide forward
until it stops (about 2).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-46: Slide Sample Loader Forward

Part Number 9140300 August 1998

4 - 47

7#5HPRYDO#)#5HSODFHPHQW# Tower Assembly (RR-8)


5. Cut the two tie wraps around cable
bundle in front of flow cell panel.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-47: Tower Cable Tie Wraps

Part Number 9140300 August 1998

4 - 48

7#5HPRYDO#)#5HSODFHPHQW# Tower Assembly (RR-8)


6. Disconnect J1, J8, J10 and J13 from
the SHM Bd mounted to the flow cell
panel.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-48: Tower Assembly Cables

Part Number 9140300 August 1998

4 - 49

7#5HPRYDO#)#5HSODFHPHQW# Tower Assembly (RR-8)


7. Perform the following steps:
a. Disconnect the tubing at aspiration
probe and wash block

Figure 4-49: Tower Assembly Tubing

b. Remove the glass bubble trap (2


screws). Do not disconnect tubing.
c. Lift the tubing from under the clip.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 50

7#5HPRYDO#)#5HSODFHPHQW# Tower Assembly (RR-8)


8. Remove the three hex head (7/64
allen) screws from the base of the
Tower Assembly and lift the assembly
out.

Figure 4-50: Tower Assembly Removal

NOTE: Long screw at base.

Replacement Procedure
Replace by following the above steps in
reverse order.

Verification Procedure
See Verification Matrix, Section 5.2.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 51

7#5HPRYDO#)#5HSODFHPHQW# HGB Flow Cell (RR-9)

HGB Flow Cell (RR-9)

Figure 4-51: Front Access Doors

Removal Procedure
1. With analyzer power off, open left and
right access doors and remove front
cover.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 52

7#5HPRYDO#)#5HSODFHPHQW# HGB Flow Cell (RR-9)


2. Remove tubing from the flow cell: two
in front, one on top and one at the
base.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-52: HGB Flow Cell Tubing

Part Number 9140300 August 1998

4 - 53

7#5HPRYDO#)#5HSODFHPHQW# HGB Flow Cell (RR-9)


3. Disconnect the inline electrical
connection.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-53: HGB Flow Cell Electrical Connection

Part Number 9140300 August 1998

4 - 54

7#5HPRYDO#)#5HSODFHPHQW# HGB Flow Cell (RR-9)


4. Remove the two screws on top of the
HGB flow cell to remove the flow cell
cover.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-54: HGB Flow Cell Cover Screws

Part Number 9140300 August 1998

4 - 55

7#5HPRYDO#)#5HSODFHPHQW# HGB Flow Cell (RR-9)


5. Remove the two screws at back of the
flow cell to remove it.

Figure 4-55: HGB Flow Cell Removal

Replacement Procedure
Replace by following the above steps in
reverse order.

Verification Procedure
See Verification Matrix, Section 5.2.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 56

7#5HPRYDO#)#5HSODFHPHQW# Sample Loader (RR-10)

Sample Loader (RR-10)

Figure 4-56: Front Access Doors

Removal Procedure
Cell-Dyn 3200 Analyzers built after May
1998 do not require sample loader removal
to access front panel.
Follow Part A to access front panel. Follow
Parts A and B to remove and replace the
loader.

Part A
1. With analyzer power off, open the left
and right access doors and remove
the safety cover.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 57

7#5HPRYDO#)#5HSODFHPHQW# Sample Loader (RR-10)


2. Remove the left and right panels from
the rear rail of the sample loader.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-57: Sample Loader Panels

Part Number 9140300 August 1998

4 - 58

7#5HPRYDO#)#5HSODFHPHQW# Sample Loader (RR-10)


3. Remove the screw at the top of the
tower assembly.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-58: Tower Assembly Screw

Part Number 9140300 August 1998

4 - 59

7#5HPRYDO#)#5HSODFHPHQW# Sample Loader (RR-10)


4. Loosen the two screws at each end of
the sample loader just one turn.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-59: Sample Loader End Screws

Part Number 9140300 August 1998

4 - 60

7#5HPRYDO#)#5HSODFHPHQW# Sample Loader (RR-10)


5. Lift sample loader and slide forward
until it stops (about 2).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-60: Slide Sample Loader Forward

Part Number 9140300 August 1998

4 - 61

7#5HPRYDO#)#5HSODFHPHQW# Sample Loader (RR-10)


Part B

Figure 4-61: Tower Assembly Base Screws

1. Remove the three hex head screws


(7/64 allen) from the base of the
tower assembly.
NOTE: Larger screw at base.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 62

7#5HPRYDO#)#5HSODFHPHQW# Sample Loader (RR-10)


2. Release the clip around the tower
assembly tubing. Disconnect the two
tubes as shown in the inset. Lay the
assembly on top of the instrument.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-62: Tower Assembly Tubing

Part Number 9140300 August 1998

4 - 63

7#5HPRYDO#)#5HSODFHPHQW# Sample Loader (RR-10)


3. Disconnect the vacuum and pressure
connections by pressing on the metal
clip.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-63: Vacuum & Pressure Connections

Part Number 9140300 August 1998

4 - 64

7#5HPRYDO#)#5HSODFHPHQW# Sample Loader (RR-10)


4. Remove plastic cover from SHM.
Disconnect all cables from SHM.
Remove cable clamp (1 screw).

Figure 4-64: SHM Cables

NOTE: Cables are marked for proper


reassembly.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 65

7#5HPRYDO#)#5HSODFHPHQW# Sample Loader (RR-10)


5. Remove cable clamps. Set aside
ribbon cable (J16) and multicolored
cable (J12).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-65: Remove Cable Clamps

Part Number 9140300 August 1998

4 - 66

7#5HPRYDO#)#5HSODFHPHQW# Sample Loader (RR-10)


6. Remove loader surround cover by
loosening front three screws and
removing left and right end screws (2
at each end).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-66: Sample Loader Cover Removal

Part Number 9140300 August 1998

4 - 67

7#5HPRYDO#)#5HSODFHPHQW# Sample Loader (RR-10)


7. Remove loader mounting screws (two
at each end). Lift loader from brackets.

Figure 4-67: Sample Loader Mounting Screws

Replacement Procedure
Replace by following the above steps in
reverse order.

Verification Procedure
See Verification Matrix, Section 5.2.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 68

7#5HPRYDO#)#5HSODFHPHQW# Syringe Driver Assembly (RR-11)

Syringe Driver Assembly


(RR-11)

Figure 4-68: Front Access Doors

Removal Procedure
1. With analyzer power off, open the left
and right access doors and remove
the safety cover.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 69

7#5HPRYDO#)#5HSODFHPHQW# Syringe Driver Assembly (RR-11)


2. Remove the right panel from the rear
rail of the sample loader.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-69: Sample Loader Right Panel

Part Number 9140300 August 1998

4 - 70

7#5HPRYDO#)#5HSODFHPHQW# Syringe Driver Assembly (RR-11)


3. Remove the screw at the top of the
tower assembly.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-70: Tower Assembly Screw

Part Number 9140300 August 1998

4 - 71

7#5HPRYDO#)#5HSODFHPHQW# Syringe Driver Assembly (RR-11)


4. Loosen the two screws at each end of
the sample loader just one turn.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-71: Sample Loader End Screws

Part Number 9140300 August 1998

4 - 72

7#5HPRYDO#)#5HSODFHPHQW# Syringe Driver Assembly (RR-11)


5. Lift sample loader and slide forward
until it stops (about 2).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-72: Slide Sample Loader Forward

Part Number 9140300 August 1998

4 - 73

7#5HPRYDO#)#5HSODFHPHQW# Syringe Driver Assembly (RR-11)


6. Unscrew the luer fittings from top of
each syringe.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-73: Luer Fittings

Part Number 9140300 August 1998

4 - 74

7#5HPRYDO#)#5HSODFHPHQW# Syringe Driver Assembly (RR-11)


7. Remove the four mounting screws for
whichever of the two syringe driver
assemblies needs replacing. (Right
side mounting screws shown here.)
Then remove the spacer bar (two
screws) between the two syringe
driver assemblies.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Figure 4-74: Syringe Driver & Spacer Bar

Part Number 9140300 August 1998

4 - 75

7#5HPRYDO#)#5HSODFHPHQW# Syringe Driver Assembly (RR-11)


8. Pull out the drive assembly and
disconnect the ribbon cables on each
side.

Figure 4-75: Chopper Driver Ribbon Cables

Replacement Procedure
Replace by following the above steps in
reverse order.

Verification Procedure
See Verification Matrix, Section 5.2.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

4 - 76

8#0#9HULILFDWLRQ#3URFHGXUHV Table of Contents

8#0#9HULILFDWLRQ#3URFHGXUHV
Table of Contents
5.1
5.2
5.3

How to Use This Chapter . . . . . . . . . . . . . . . . . . . . . 2


Verification Matrix . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Verification Procedures . . . . . . . . . . . . . . . . . . . . . . 5
VP-1 System Voltage Verification/Adjustment. . . . . . . . 5
VP-2 A/D Verification/10 Volt Reference
Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
VP-3 MAM Reference Voltage Verification/
Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
VP-4 VPM Reference Voltage Verification/
Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
VP-5 HGB Current Verification/Adjustment . . . . . . . . . . 8
VP-6 Motor Power Verification . . . . . . . . . . . . . . . . . . . 8
VP-7 Motor Operation Verification. . . . . . . . . . . . . . . . . 9
VP-8 Motor Homing Verification . . . . . . . . . . . . . . . . . . 9
VP-9 Solenoid Bank Verification . . . . . . . . . . . . . . . . . 10
VP-10 Individual Solenoid Verification. . . . . . . . . . . . . . 10
VP-11 Bar Code Reader Verification/Alignment . . . . . . 10
VP-12 Specimen Sensor #2 Verification/Adjustment . . 11
VP-13 LSI Loopback Verification. . . . . . . . . . . . . . . . . . 11
VP-14 MAM & SPM Signal Generator Verification . . . . 12
VP-15 Vacuum & Pressure Retention Verification . . . . 12
VP-16 Vacuum & Pressure Level
Verification/Adjustment. . . . . . . . . . . . . . . . . . . . 13
VP-17 Vacuum & Pressure Recovery Verification. . . . . 13
VP-18 Laser Replacement/Alignment Procedure . . . . . 14
VP-19 Front/Rear Mirror Replacement/Alignment
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
VP-20 Optical Flow Cell Verification/Fine Alignment. . . 17
VP-21 Optical Flow Cell Coarse Alignment . . . . . . . . . . 21

CELL-DYN 3200 SYSTEM SERVICE MANUAL

VP-22
VP-23
VP-24
VP-25
VP-26
VP-27
VP-28
VP-29
VP-30
VP-31
VP-32
VP-33
VP-34
VP-35
VP-36
VP-37
VP-38
VP-39
VP-40
VP-41
VP-42
VP-43
VP-44
VP-45
VP-46
VP-47
VP-48
VP-49

PMT Dynode Voltage Verification/Adjustment . . 22


WBC Gain Verification/Adjustment . . . . . . . . . . . 23
WBC OPTI-CAL Verification/Adjustment. . . . . . . 24
NOC Gain Adjustment. . . . . . . . . . . . . . . . . . . . . 26
RBC/PLT 0 Gain Verification/Adjustment . . . . . 27
RBC/PLT 10 Gain Verification/Adjustment . . . . 28
MAM 0 & 10 Log Amplifier Verification . . . . . . . 29
0 MAM Log Amplifier Verification/Adjustmen. . . 32
10 MAM Log Amplifier Verification/
Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Aspirate/Vent Needle Verification . . . . . . . . . . . . 34
GS2 Stop Solenoid Verification . . . . . . . . . . . . . . 35
Bar Code Spin Assembly Verification . . . . . . . . . 35
Tube Height Sensors (S1/S2) Verification. . . . . . 35
Mixer Up/Down Verification . . . . . . . . . . . . . . . . . 36
Mixer Head Rotation Verification . . . . . . . . . . . . . 36
Mixer Bladders Verification . . . . . . . . . . . . . . . . . 37
Rack Advance & Tube Sensors Verification . . . . 37
Cross Transfer Arms & Rack Sensors
Verification. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Rack Bar Code Labels Verification . . . . . . . . . . . 38
Mixer Bladders Pressure Verification/
Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Shear Valve Driver Lubrication Procedure . . . . . 39
Optics Bench Cleaning Procedure . . . . . . . . . . . 40
Optical Flow Cell Cleaning & De-Bubble
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Optical Flow Cell Wetting Procedure. . . . . . . . . . 45
Laser Power Output Verification . . . . . . . . . . . . . 46
Optical Offsets Verification . . . . . . . . . . . . . . . . . 47
Shear Valve Cleaning Procedure . . . . . . . . . . . . 47
HGB Flow Cell Cleaning Procedure . . . . . . . . . . 48

Part Number 9140300 August 1998

5-1

8#0#9HULILFDWLRQ#3URFHGXUHV 5.1 How to Use This Chapter


VP-50 Open Mode Aspiration Path Cleaning
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
VP-51 Closed Mode Aspiration Path Cleaning
Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
VP-52 Power-Up CPU/DCM 7-Segment LED
Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
VP-53 Software Installation Procedure . . . . . . . . . . . . .
VP-54 Hard Drive Verification . . . . . . . . . . . . . . . . . . . .
VP-55 Floppy Drive Verification. . . . . . . . . . . . . . . . . . .
VP-56 Background Counts Verification . . . . . . . . . . . . .
VP-57 System CV Verification. . . . . . . . . . . . . . . . . . . .
VP-58 System Calibration Verification/Adjustment . . . .
VP-59 Vacuum Accumulator #1 Rinsing Procedure . . .
VP-60 Vacuum Accumulator #2 Rinsing Procedure . . .
VP-61 Hard Drive Formatting . . . . . . . . . . . . . . . . . . . .
VP-62 DOS Installation . . . . . . . . . . . . . . . . . . . . . . . . .
VP-63 CS/SL SETUP . . . . . . . . . . . . . . . . . . . . . . . . . .
VP-64 Backup Procedure . . . . . . . . . . . . . . . . . . . . . . .
VP-65 Restore Setup. . . . . . . . . . . . . . . . . . . . . . . . . . .
VP-66 List Mode Data Collection. . . . . . . . . . . . . . . . . .
VP-67 Download HSSL Com. . . . . . . . . . . . . . . . . . . . .

User and System Conventions


49
50

Menu selections are identified by square bullets. For example:


51
51
51
52
53
53
54
54
55
56
57
57
58
58
58
59

5.1 How to Use This Chapter


This chapter contains:

Verification Matrix containing all VPs required to ensure


System integrity after a part is replaced

Step-by-step illustrated procedures to verify, align, calibrate,


and maintain components, assemblies, and functions of the
CD3200 Analyzer and Autoloader

Menu Selections
1. From Main Menu, press
DIAGNOSTICS
MORE
TOWER TEST
or:
a. Press

ACCEPT

to enter the new factors for the parameters that need


calibration.

User Entries
User-typed entries are shown in bold, italicized type. For
example:
a. At C:\ prompt type CD\RAWFILE, and press

ENTER

b. Type DIR, and Press

ENTER

A list of conditions indicating the need to perform a


particular procedure
Required tools and materials for each procedure

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5-2

8#0#9HULILFDWLRQ#3URFHGXUHV 5.1 How to Use This Chapter

5.2 Verification Matrix

REPLACED ASSEMBLY

ASSOCIATED VPs
(Perform In Order Listed)
VP-35, VP-36, VP-37, VP-38,
VP-39, VP-40, VP-41

REPLACED ASSEMBLY

ASSOCIATED VPs
(Perform In Order Listed)

Sample Handler Module #2

0 Degree Preamp

VP-23, VP-19, VP-26, VP-56,


VP-57, VP-58

HGB Flow Cell

10 Degree Preamp

VP-23, VP-19, VP-27, VP-56,


VP-57, VP-58

Motor Driver Module

VP-7

Sample Sensor #2

VP-12

90 Degree PMT Preamp

VP-22, VP-23, VP-56, VP-57,


VP-58

Pump Relay Module

VP-17, VP-16

VP-22, VP-23, VP-56, VP-57,


VP-58

Barcode Reader

VP-10, VP-40

90 Degree PMT Preamp

Aspiration Tower

VP-31, VP-32, VP-33, VP-34

Main Amplifier Module

VP-3, VP-28, VP-23, VP-26,


VP-27, VP-56, VP-57, VP-58

Sample Loader

VP-35, VP-36, VP-37, VP-38,


VP-39, VP-40, VP-41

Signal Processor Module

VP-23,VP-26, VP-27, VP-56,


VP-57, VP-58

Optics Bench

CPU/DCM

VP-67, VP-52, VP-2, VP-56,


VP-57, VP-58

VP-45, VP-22, VP-20, VP-23,


VP-24, VP-25, VP-26, VP-27,
VP-56, VP-57, VP-58

Laser Head

VP-18,VP-20, VP-23, VP-24,


VP-25, VP-26, VP-27, VP-56,
VP-57, VP-58

Optics Bench Mirror

VP-19, VP-20, VP-23, VP-24,


VP-25, VP-26, VP-27, VP-56,
VP-57, VP-58
VP-19, VP-20, VP-23, VP-24,
VP-25, VP-26, VP-27

Flow Control Module


Vacuum/Pressure Module
Motor Processor Module
Power Supply Module

VP-5, VP-9
VP-4, VP-16, VP-56, VP-57,
VP-58
VP-6, VP-7

Shear Valve Driver Module

VP-1, VP-56, VP-57, VP-58

VP-5, VP-56, VP-57, VP-58


VP-7, VP-57

Laser Power Supply

VP-46

Optical Flow Cell

Solenoid Driver Module


SDM/CDM

VP-9

Syringe Driver Assembly

VP-6, VP-7, VP-56, VP-57,


VP-58

Peri-Pump Assembly

VP-6, VP-8, VP-7, VP-56,


VP-57, VP-58

Sample Handler Module #1

VP-31, VP-32, VP-33, VP-34

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5-3

8#0#9HULILFDWLRQ#3URFHGXUHV 5.1 How to Use This Chapter


REPLACED ASSEMBLY

ASSOCIATED VPs
(Perform In Order Listed)

S. Valve Center Ceramic

VP-1, VP-56, VP-57, VP-58

Hard Disk Drive


Floppy Disk Drive

VP-53, VP-54
VP-55

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5-4

8#0#9HULILFDWLRQ#3URFHGXUHV VP-1 System Voltage Verification/Adjustment

5.3 Verification Procedures


VP-1 System Voltage Verification/Adjustment
Required Tools & Materials:

Table 5-1

Voltage Specifications

Test Point

Function

Range

Ripple

PSM-TP4

Analog Ground

N/A

N/A

PSM-TP2

+12 VDC UREG

15.0V-19.6V

< 3.0V PP

# 2 Phillips Screwdriver

PSM-TP1

+24 VDC UREG

28.9V-37.7V

< 7.0V PP

Digital Voltmeter

PSM-TP7

N/A

N/A

N/A

Clip Leads

PSM-TP6

N/A

N/A

N/A

Potentiometer Adjustment Tool

PSM-TP3

+15 VDC

14.6V-15.4V

< 75MV PP

PSM-TP5

-15 VDC

14.6V-15.4V

< 75MV PP

N/A

-12 VDC

11.50-12.50V

<75MV PP

N/A

+12 VDC

11.50-12.50V

<75MV PP

N/A

+5 VDC

5.00V-5.40V

<75MV PP

N/A

+24 VDC REG

24.0-25.0

<75MV PP

Verification Procedure:
1. Open right side door to gain access to power supply.
2. Refer to Figure 5-1. Connect voltmeter ground lead to TP4
on power supply module.
3. Verify the voltages according to Table 5-1.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5-5

8#0#9HULILFDWLRQ#3URFHGXUHV VP-2 A/D Verification/10 Volt Reference Adjustment


Figure 5-1

Power Supply Voltage Testpoints

REAR

TO P

15 A S B (+ 1 2V )
F1
ALARM

T P 1 + 24 V

F2

-12V

T P 2 + 12 V

15 A S B (+ 2 4V )
PSM

+ 12 V

T P 3 + 15 V
TP4

GND

TP5

-15V

+ 24 V
GND
+ 5V

10 0V
TP6 RET
+ 10 0V
TP7

VP-2 A/D Verification/10 Volt Reference


Adjustment
The CELL-DYN 3200 utilizes an A/D converter to convert all
critical voltages levels to a 12-bit digital code. This code is read
by the computer and these voltages are then displayed on the
VOLTAGE READINGS screen in DIAGNOSTICS. The
following procedure verifies the accuracy of this A/D Converter
by using the A/D to read the 9.901 volt reference generated on
the CPU/DCM and is also used to set the level of the 10 volt
reference.
CELL-DYN 3200 SYSTEM SERVICE MANUAL

3 2 0 76

Required Tools & Materials:

# 2 Phillips Screwdriver

Digital Voltmeter

Clip Leads

Potentiometer Adjustment Tool

Verification Procedure:
1. Open right side door to gain access to the CPU/DCM.
Part Number 9140300 August 1998

5-6

8#0#9HULILFDWLRQ#3URFHGXUHV VP-3 MAM Reference Voltage Verification/Adjustment


2. Locate the CPU/DCM on the right side door and connect
the positive lead of the DVM to TP1 and the negative lead
to TPAGND.
3. Adjust R4 for a reading of 10.00 volts +/- 0.01V.
4. Move the positive lead to TP3 (9.901V reference) and
verify that the meter reading is 9.90 volts +/- 0.03V.
5. Move the positive lead to TP2 (99mV reference) and verify
that the meter reading is 0.099 volts +/- 0.003V.
6. From Main Menu, press

Verification Procedure:
1. Connect positive lead of DVM to TP2 and negative to TP7.
2. If necessary, adjust R86 for a reading of 10.00 volts +/0.01V.

VP-4 VPM Reference Voltage


Verification/Adjustment
Required Tools & Materials:

DIAGNOSTICS

# 2 Phillips Screwdriver

MORE

Digital Voltmeter

VOLTAGE READINGS

Clip Leads

Potentiometer Adjustment Tool

7. Press [VOLTAGE READINGS] and observe the 9.901V


Reference on the SPM. Verify by repeatedly pressing the
key that the voltage displayed is 9.90 volts +/- 0.05V.

Verification Procedure:

VP-3 MAM Reference Voltage


Verification/Adjustment

1. Open flow panel door to gain access to the VPM.

Required Tools & Materials:

3. If necessary, adjust R16 for a reading of 10.00 volts +/0.01V.

# 2 Phillips Screwdriver

Digital Voltmeter

Clip Leads

Potentiometer Adjustment Tool or Small Slotted


Screwdriver

CELL-DYN 3200 SYSTEM SERVICE MANUAL

2. Connect positive lead of DVM to TP2 (VREF+) and


negative to TP1 (VREF-).

Part Number 9140300 August 1998

5-7

8#0#9HULILFDWLRQ#3URFHGXUHV VP-5 HGB Current Verification/Adjustment

VP-5 HGB Current Verification/Adjustment


This procedure adjusts the HGB LED current to yield a HGB
output voltage of 5.00 volts with the HGB Flow Cell filled with
Reference Solution.

6. Move the cursor to HGB CURRENT; enter new setting,


and press [ENTER]. A change of 50 will yield a voltage
change of approximately 0.3 volts.
7. Press
SET ANALYZER

Required Tools & Materials:

RETURN

None

Verification Procedure:

RETURN
8. Press
VOLTAGE READINGS

1. From Main Menu, press

and repeat steps 2 through 8 until the voltage is within


specifications.

DIAGNOSTICS
MORE
VOLTAGE READINGS
2. Move the cursor to HGB output, and press
SELECT
FINISH SELECT
3. Run one background count to insure that the HGB mixing
chamber is full of liquid.
4. Verify that the HGB OUTPUT voltage is 5.00 volts
+0.2/-0.00 volts. If the voltage is within range, skip steps 5
through 8.
5. Press
MORE
to the first diagnostic screen, and press
F12
F1

VP-6 Motor Power Verification


This procedure verifies the winding current of the system
stepper motors.

Required Tools & Materials:

None

Verification Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
MOTOR OPERATION
2. Press
MOTOR PWR CHECKING
and wait for the display.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5-8

8#0#9HULILFDWLRQ#3URFHGXUHV VP-7 Motor Operation Verification


3. Verify each motor meets the specifications shown in Table
5-2.
Table 5-2

2. Press
EXERCISE MOTORS
type the letter of the suspect assembly, and press

Motor Power Specifications

ENTER

Phase 0

Phase 0

Phase 0

Phase 3

Motor

Low

Medium

High

High

1.5-2.1

3.4-4.2

4.9-5.9

4.9-5.9

.6-1.2

2.5-3.1

4.2-5.0

4.2-5.0

.6-1.2

2.5-3.1

4.2-5.0

4.2-5.0

VP-8 Motor Homing Verification

.6-1.2

2.5-3.1

4.2-5.0

4.2-5.0

1.5-2.1

3.4-4.2

4.9-5.9

4.9-5.9

This procedure allows visual verification of the homing


operation of the system motors by running a homing routine.

1.5-2.1

3.4-4.2

4.9-5.9

4.9-5.9

3. Verify that the assembly moves smoothly without stopping


or jerking.
4. Use the [SHEAR VALVE] and [Y VALVE] keys to exercise
the shear valve and Y valve.

Required Tools & Materials:

VP-7 Motor Operation Verification


This procedure allows visual verification of the operation of the
system motors by running an exercise routine.

None

Verification Procedure:
1. From Main Menu, press
DIAGNOSTICS

Required Tools & Materials:

None

Verification Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
MOTOR OPERATION
CELL-DYN 3200 SYSTEM SERVICE MANUAL

MORE
MOTOR OPERATION
2. Press
HOME MOTORS
type the letter of the suspect assembly, and press
ENTER
3. Verify that the assembly moves smoothly without stopping
or jerking.
Part Number 9140300 August 1998

5-9

8#0#9HULILFDWLRQ#3URFHGXUHV VP-9 Solenoid Bank Verification

VP-9 Solenoid Bank Verification


This procedure allows visual verification of the operation of a
bank of eight solenoids by running an exercise routine.

Verification Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE

Required Tools & Materials:

None

SOLENOID OPERATION
2. Press
STEP SOLENOID

Verification Procedure:

type the number of the starting solenoid, and press

1. From Main Menu, press

ENTER

DIAGNOSTICS
MORE
SOLENOID OPERATION
2. Press
CYCLE BANK
type the number of the bank, and press
ENTER

3. Use the UP/DOWN ARROW KEYS, and verify that the


solenoids are opened when commanded.

VP-11 Bar Code Reader Verification/Alignment


Required Tools & Materials:

Specimen Tube With Bar Code Label

3. Verify that the solenoids are opened in ascending order.

Verification Procedure:

VP-10 Individual Solenoid Verification


This procedure allows visual verification of the operation of a
bank of eight solenoids by running an exercise routine.

Required Tools & Materials:

None

1. From Main Menu, press


DIAGNOSTICS
MORE
BAR CODE ALIGNMENT
2. Record the tube bar code number, and place tube at the
read station.
3. Press
BAR CODE ALIGNMENT

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 10

8#0#9HULILFDWLRQ#3URFHGXUHV VP-12 Specimen Sensor #2 Verification/Adjustment


4. Verify that the number displayed on the screen matches
the recorded number, and the read rate is constant.
5. If necessary, loosen the locking screws, and align the
position of the reader until the read rate is constant.
6. Tighten the locking screws and re-verify.

VP-12 Specimen Sensor #2


Verification/Adjustment
Required Tools & Materials:

CELL-DYN 3200 Diluent/Sheath

Low Abnormal Control

DVM with Clip Leads

Potentiometer Adjustment Tool

Verification Procedure:
1. Remove top and top right covers.
2. Run controls and insure that results are within
specifications.
3. Create a specimen dilution using LOW ABNORMAL
CONTROL material and CELL-DYN 3200 Diluent/Sheath
that gives an RBC value of 0.8MEG/ul, +/- .03.
4. Connect the positive lead of the DVM to TP1 and the
negative lead to TP3 on the specimen sensor board.

NOTE: The voltage at TP4 should be <0.50V when air or


liquid is passing through the sensor, and >3.00V with the
diluted control.
7. Run a background count, and verify a reading of (0.50V.
8. While observing the tubing through specimen sensor #2,
place the diluted low control under the open probe, and
manually open valve #12 until the dilution is aspirated
approximately 2 inches past specimen sensor #2.
9. Verify a reading of >3.00V.
10. Manually open solenoid 13 to remove blood from lines,
and run a background count.
11. Run another background count, and verify that the LED on
the sensor board does not flicker.
12. Disconnect the DVM from the specimen sensor board.

VP-13 LSI Loopback Verification


Required Tools & Materials:

Loopback Connector PN 9211532

Verification Procedure:
1. Turn power off, install loopback connector at COM1 (top
serial connector) on PC module, and power-up and
initialize instrument.

5. Adjust R1 for a reading of 2.00 +/-0.01 volts.


6. Move positive lead to TP4.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 11

8#0#9HULILFDWLRQ#3URFHGXUHV VP-14 MAM & SPM Signal Generator Verification


2. From Main Menu, press
DIAGNOSTICS
MORE
SERIAL TEST
3. Press
TRANSMIT MESSAGE
and verify that the message received matches the
message sent: CELL-DYN serial interface test.
4. Press
STOP TRANSMISS
RETURN
RETURN
5. Turn power off, and remove loopback connector.

VP-15 Vacuum & Pressure Retention


Verification
This procedure tests the ability of the system to retain the
appropriate vacuum and pressure levels. A failure indicates a
leak in the system.

Required Tools & Materials:

None

Verification Procedure:
1. Ensure that the instrument is in the READY mode.
2. Verify that off-time of the vacuum pump is 2 minutes or
greater between automatic recovery cycles.
3. Verify that off-time of the pressure pump is 2 minutes or
greater between automatic recovery cycles.

VP-14 MAM & SPM Signal Generator


Verification
Required Tools & Materials:

None

Verification Procedure:

Not Available At This Time

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 12

8#0#9HULILFDWLRQ#3URFHGXUHV VP-16 Vacuum & Pressure Level Verification/Adjustment

VP-16 Vacuum & Pressure Level


Verification/Adjustment

to the first diagnostic screen and press


F12
F1

Required Tools & Materials:

Use the following equation to calculate the new setting(s).

None

New Setting = Target Level X Current Setting


Actual Level

Verification Procedure:
1. Ensure that the instrument is in the Open Sampler mode.

6. Move the cursor to the appropriate vacuum(s) or


pressure(s), enter new setting(s) and press
ENTER

2. From Main Menu, press


DIAGNOSTICS

7. Press

MORE

SET ANALYZER

VOLTAGE READINGS

RETURN

3. Observe the vacuum and pressure levels and compare


with Table 5-3.
Table 5-3

Vacuum & Pressure Specifications

Vacuum/Pressure

Specification

Pressure #1

12.5-13.5 psi

Pressure #2

8.5-9.5 psi

Pressure #3

4.0-4.5 psi

Vacuum #1

12.5-13.5 Hg

Vacuum #2

3.1-3.5 Hg

4. If all the vacuum and pressure levels are within


specifications, do not perform steps 5 through 8.

RETURN
NOTE: If the level is reduced the vacuum or pressure
must be bled off to the new level before performing step 8.
A count cycle may be used for bleeding.
8. Go to VOLTAGE READINGS, and repeat steps 3 through
8 until the level(s) are within specifications.

VP-17 Vacuum & Pressure Recovery


Verification
This procedure tests the ability of the vacuum and pressure
pumps to return from atmosphere to the appropriate levels. A
failure indicates that the pump under test is defective or there is
a gross leak in the system.

5. Press
MORE
CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 13

8#0#9HULILFDWLRQ#3URFHGXUHV VP-18 Laser Replacement/Alignment Procedure


Required Tools & Materials:

None

Verification Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
to VACUUM TEST and PRESSURE TEST.
2. Press
VACUUM TEST
and verify that the elapsed time is 4.5 seconds or less.
3. Press
PRESSURE TEST
and verify that the elapsed time is 3.5 seconds or less.

VP-18 Laser Replacement/Alignment


Procedure
'$1*(5=#/$6(5#/,*+7 Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.

Required Tools & Materials:

# 2 Phillips Screwdriver

.050 Allen Wrench

Optics Alignment Kit Cat. #8921183801

DVM with Clip Leads

Laser Power Meter

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Verification Procedure:
1. Ensure instrument power is off. Remove top cover, both
optics covers, and open flow cell access door.
'$1*(5=#+,*+#92/7$*(#Assure the power
to the instrument is off. A high voltage charge may
remain on the laser connector with the power off.
Do not touch the male pins. Disconnect the laser
connector and short both male pins to the
instrument chassis.
2. Disconnect laser connector and short both male pins to
instrument chassis.
3. Remove Laser Clamps and replace Laser.
4. Replace clamps and tighten until snug but Laser can still
be moved.
5. Move laser body until 3/4 inch extends beyond front laser
clamp and rotate laser body until front label is on top.
6. Connect laser connector, ensure laser shutter is closed,
turn on power, and prime instrument. Allow a 15-minute
laser warm-up period.
7. Refer to Figure 5-4. Mount Power Meter in beam path.
Open Laser Shutter and verify a reading of no less
than 5 mW.
8. If you have a polarizing filter, skip step 9.
9. Scribe optics bench on right and front sides of beam
splitter/polarizer assembly, and remove assembly from
90o path.

Part Number 9140300 August 1998

5 - 14

8#0#9HULILFDWLRQ#3URFHGXUHV VP-18 Laser Replacement/Alignment Procedure


10. Place polarizing filter or beam splitter/polarizer in beam
path between laser and power meter with beam splitter
facing laser.
11. While observing power reading, rotate the laser body
clockwise. As power level decreases, change scales as
needed.
12. Rotate laser body until power is at minimum on the 20 uW
scale.
13. Tighten laser clamps and verify that power level does not
change more than +/- 3 uw and remove beam
splitter/polarizer from beam path. Laser is now aligned for
true vertical polarization.

repeated several times to attain proper alignment. Record


power level through slit and, if necessary, replace beam
splitter/polarizer in normal position.
19. Connect the negative lead of the DVM to TP2 and the
positive lead to TP1 on the 0o photodiode PCB.
20. Refer to Figure 5-2. Manually open solenoid 56 and
remove tubing from port #1 on the flow cell distribution
manifold.
Figure 5-2

Flow Cell Plumbing.


5

14. Place alignment post (#9420071) in front tooling hole and


center beam on hole using X and Y alignment screws on
rear mirror. if alignment post is not available, center beam
on cylindrical lens. Placing scotch tape on rear of
cylindrical lens will aid in centering beam.

56

WBC
FLO W
C EL L

91
3

FLO W C E LL M AN IFO LD

15. Remove alignment post and place power meter behind


forward slit.
16. Use X alignment (.050" allen wrench) screw on rear mirror
to obtain maximum power through forward slit. Maximum
power through slit should be approximately 1/5 of laser
power.
17. Use X alignment screw on front mirror to move beam
toward alignment hole in center of obscuration bar.
18. Repeat steps 16 through 18 until maximum power is being
transmitted through slit and beam is centered horizontally
on hole in center of obscuration bar. This may have to be

CELL-DYN 3200 SYSTEM SERVICE MANUAL

4
54
1

57

2
52

32 077

21. Observe the 90o slit. When two red images of the flow cell
walls appear on the slit, close solenoid 56 and reconnect
tubing to port #1.
22. Adjust the Y alignment screw on the front mirror for a
minimum reading on the DVM. This reading should be less
than 0.300 volts.
23. Adjust the Y alignment screw on the rear mirror until the
red images are in the center 1/3 of the 90o slit.
Part Number 9140300 August 1998

5 - 15

8#0#9HULILFDWLRQ#3URFHGXUHV VP-19 Front/Rear Mirror Replacement/Alignment Procedure


24. Repeat steps 22 and 23 until the red images are in the
center 1/3 of the slit and the DVM voltage is at minimum.
The minimum voltage should be less than 0.200 volts.
25. Re-check power transmitted through forward slit. If it has
changed, a slight X alignment of the rear mirror is
necessary.
26. Replace both optics covers.

2. Remove, replace, and secure defective mirror (front


or rear).
3. Place alignment post (#9420071) in front tooling hole and
center beam on hole using X and Y alignment screws on
rear mirror.
4. Remove alignment post and place power meter behind
forward slit.

27. Perform Optical Flow Cell Verification/Fine Alignment


VP-20 and, if necessary, perform Optical Flow Cell Coarse
Alignment VP-21, and repeat VP-20.

5. Use X alignment (.050" allen wrench) screw on rear mirror


to obtain maximum power through forward slit. Maximum
power through slit should be approximately 1/5 of laser
power.

VP-19 Front/Rear Mirror


Replacement/Alignment Procedure

6. Use X alignment screw on front mirror to move beam


toward alignment hole in center of obscuration bar.

'$1*(5=#/$6(5#/,*+7 Avoid direct


exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.

Required Tools & Materials:

# 2 Phillips Screwdriver

.050 Allen Wrench

Optics Alignment Kit Cat. #8921183801

DVM with Clip Leads

Laser Power Meter

7. Repeat steps 4 through 6 until maximum power is being


transmitted through slit and beam is centered horizontally
on hole in center of obscuration bar. This may have to be
repeated several times to attain proper alignment. Record
power level through slit and, if necessary, replace beam
splitter/polarizer in normal position.
8. Connect the negative lead of the DVM to TP2 and the
positive lead to TP1 on the 0o photodiode PCB.
9. Refer to Figure 5-3. Manually open solenoid 56 and
remove tubing from port #1 on the flow cell distribution
manifold.

Verification Procedure:
1. Remove top cover, both optics covers, and open flow cell
access door.
CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 16

8#0#9HULILFDWLRQ#3URFHGXUHV VP-20 Optical Flow Cell Verification/Fine Alignment


5

VP-20 Optical Flow Cell Verification/Fine


Alignment

56

'$1*(5=#/$6(5#/,*+7 Avoid direct


exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.

WBC
FL O W
CELL

91
3

FL O W C E LL M A NIFO L D
4

57

54
1

2
52

32077

Figure 5-3

Flow Cell Plumbing

10. Observe the 90o slit. When two red images of the flow cell
walls appear on the slit, close solenoid 56 and reconnect
tubing to port #1.
11. Adjust the Y alignment screw on the front mirror for a
minimum reading on the DVM. This reading should be less
than 0.300 volts.
12. Adjust the Y alignment screw on the rear mirror until the
red images are in the center 1/3 of the 90o slit.
13. Repeat steps 11 and 12 until the red images are in the
center 1/3 of the slit and the DVM voltage is at minimum.
The minimum voltage should be less than 0.200 volts.
14. Re-check power transmitted through forward slit. If it has
changed, a slight X alignment of the rear mirror is
necessary.

The alignment of the optical flow cell is one of the most critical
alignments performed on the CELL-DYN 3200. It must be
optimized so that the instrument can properly perform the WBC
5-Part Differential measurements. However, if this alignment is
not really needed, it is best that it not be performed. Therefore,
if the instrument is within the prescribed specifications, do not
attempt to align the optical flow cell.

Required Tools & Materials:

# 2 Phillips Screwdriver

7.00 Diameter Beads, PN 1606004

CELL-DYN 3200 Diluent/Sheath

Clean Container

Verification Procedure:
1. Prepare the WBC Beads solution.
a. Place 2mL of diluent in a clean container.
b. Add 15 drops of 7.00 beads and mix well.
2. From Main Menu, press

15. Replace both optics covers.

DIAGNOSTICS

16. Perform Optical Flow Cell Verification/Fine Alignment


VP-20 and, if necessary, perform Optical Flow Cell Coarse
Alignment VP-21, and repeat VP-20.

MORE

CELL-DYN 3200 SYSTEM SERVICE MANUAL

WBC DATA
Part Number 9140300 August 1998

5 - 17

8#0#9HULILFDWLRQ#3URFHGXUHV VP-20 Optical Flow Cell Verification/Fine Alignment


7. Loosen the locking screw on the lower (y-axis)
micrometer, and cover the opening with black cloth to
block ambient light.

3. Press
CALC CV
4. Place Beads solution under probe and press TOUCH
PLATE to aspirate Beads and run a count cycle.
5. Refer to Table 5-4 and compare the CVs to those
displayed on the screen. If the displayed CVs are less than
those from the table, the flow cell does not need
alignment.
NOTE: If any or all of the CVs are out-of-specification, the
flow cell must be completely aligned. The 0o and 10o
channels must be aligned first by adjusting the Y-axis
alignment (lower) micrometer and then the 90o and 90o
depolarized must be aligned by adjusting the X-axis
alignment (right) micrometer.
Table 5-4

WBC Mean Channel & CV Specification

Channel

Mean

Range

CV

0o

35

32-38

<3.5%

10o

65

60-70

<3.5%

90o

* Derived From Opti-Cal +/- 5 Channels <12.5%

90 Dep. * Derived From Opti-Cal +/- 5 Channels <12.5%


NOTE: * These values must be obtained from the
Beads auto-gain default table, or by performing the
Opti-Cal procedure.
6. Open the flow cell access door, located at the top left of
the Analyzer.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

8. Place the 7.00 beads solution under the probe, and


press
EXTENDED WBC COUNT
START
to aspirate the Beads and start the cycle.
9. Observe the 0o and 10o display on the left side of the
screen. When dots appear on the screen, adjust the lower
micrometer until the tightest possible grouping of dots and
lowest possible CVs are attained. If the cycle stops before
this is accomplished, repeat step 8 and try again.
10. Place Beads solution under probe and START SWITCH
to aspirate Beads and run a count cycle.
11. Press
CALC CV
when the cycle is completed. If the 0o and 10o CVs are
within specifications, continue with the procedure. If not,
repeat steps 8 through 10.
NOTE: If repeated alignment does not bring the CVs
within specifications, further troubleshooting is required to
isolate the problem. The first considerations are a
contaminated or defective flow cell or a defective laser.
The last consideration should be that of a misaligned
optical bench, since once properly aligned and secured,
none of the assemblies should move under normal
conditions.

Part Number 9140300 August 1998

5 - 18

8#0#9HULILFDWLRQ#3URFHGXUHV VP-20 Optical Flow Cell Verification/Fine Alignment


12. Loosen the locking screw on the right (x-axis) micrometer,
and cover the opening with black cloth to block ambient
light. Then press the START key to aspirate the Beads
and start the cycle.
13. Observe the 90o and 90o depolarized display on the right
side of the screen. When dots appear on the screen,
adjust the micrometer to attain the greatest deflection to
the right and up. CVs are not as important as in the 0o and
10o channels.
14. Place Beads solution under probe and press START
SWITCH to aspirate Beads and run a count cycle.
15. Press the key when the cycle is completed. If the 90o and
90o depolarized CVs are within specifications, the
procedure is completed. If not, repeat steps 12 through 15.
16. Tighten the locking screws and record the setting for future
reference.
17. Perform PMT Dynode Voltage Verification/Adjustment
VP-22.
18. Perform WBC Gain Verification/Adjustment VP-23.
19. Perform WBC OPTI-CAL Verification/Adjustment (VP-24).
20. Perform NOC Gain Adjustment VP-25.
21. Perform RBC/PLT 0o Gain Verification/Adjustment VP-26.
22. Perform RBC/PLT 10o Gain Verification/Adjustment
VP-27.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 19

8#0#9HULILFDWLRQ#3URFHGXUHV VP-20 Optical Flow Cell Verification/Fine Alignment


Figure 5-4

CELL-DYN 3200 Optics Bench


R ear M irror

X A xis

Laser S trap

Laser S trap

5 M illiw att H E N E Laser


Vertical P olarization
632.8 N anom eters

90 oD epolarized P M T

.75"
P lace B e am splitter/
P olarizer here during
Laser po larization.

90 oP M T

B eam sp litter/P olarizer

Y
X

P lace P o w er M eter
H ead he re during
Laser po larization.

A
x
i
s

90 oS lit/F ield Lens

M irror R e ar View

F ixture H oles
90 oO bjective Lens
10 o D etector

C ylinderc al Lens

P erforate d M irror M ount


Tooling
H ole

F orw ard S lit

Im aging Lens

0 oD etector

F low C ell
Y -A xis A lignm ent M ic.
F ront M irror

CELL-DYN 3200 SYSTEM SERVICE MANUAL

O bscura tion B ar
X -A xis A lignm ent M ic.

Part Number 9140300 August 1998

F orw ard S catter A ssy.


3 2 078

5 - 20

8#0#9HULILFDWLRQ#3URFHGXUHV VP-21 Optical Flow Cell Coarse Alignment

VP-21 Optical Flow Cell Coarse Alignment


'$1*(5=#/$6(5#/,*+7 Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.

Required Tools & Materials:

7.00 Diameter Beads

CELL-DYN 3200 Diluent/Sheath

Clean Container

5. Continue rotating CCW until the pattern of the front wall is


observed, and record the micrometer setting (Figure
5-5-C).
Figure 5-5

Beam Images
"B "
R ear W all

"A "
C lear Q u artz

S catter P attern

O bscuration B ar
"C "
F ront W all

"D "
F low C ell C entered

Verification Procedure:
1. Prepare the Bead solution.

320 79

B rightest Im age

a. Place 2mL of diluent in a clean container.


b. Add 15 drops of 7.00 beads and mix well.
2. Remove top cover and flow cell access cover.
3. Loosen the locking screw on the lower (y-axis)
micrometer, and back off (CW viewed from top) on the
micrometer (Figure 5-5) until the beam is striking the flow
cell at a point to the rear of the flow cell channel. The
scatter pattern on the obscuration bar should resemble
that in Figure 5-5-A. Placing a piece of white paper in front
of the obscuration bar will aid in viewing the scatter
pattern.
4. While observing the scatter pattern, rotate the micrometer
CCW until the pattern of the rear wall of the flow cell
channel is observed, and record the micrometer setting
(Figure 5-5-B).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

6. Rotate the micrometer CW to a setting half-way between


those recorded in steps 3 and 4. This should coarsely
center the flow cell channel on the beam; the pattern will
resemble that of Figure 5-5-D and will be at the brightest
level.
7. Cover the opening with black cloth to block ambient light.
8. From Main Menu, press
DIAGNOSTICS
MORE
WBC DATA
9. Press
CALC CV

Part Number 9140300 August 1998

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8#0#9HULILFDWLRQ#3URFHGXUHV VP-22 PMT Dynode Voltage Verification/Adjustment


10. Place Beads solution under probe and press START
SWITCH to aspirate Beads and run a count cycle.
11. If there is data in both displays, perform the fine alignment
procedure VP-20. If there is no data in either display,
Repeat steps 5 through 9.

2. From Main Menu, press


DIAGNOSTICS
MORE
WBC DATA
3. Press

VP-22 PMT Dynode Voltage


Verification/Adjustment
'$1*(5=#/$6(5#/,*+7 Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.

Required Tools & Materials:

# 2 Phillips Screwdriver

3/16 Allen Wrench

7.00 Diameter Beads, PN 1606004

CELL-DYN 3200 Diluent/Sheath

Clean Container

Verification Procedure:
1. Prepare the WBC Beads solution:

CALC CV
4. Place Beads solution under probe and press START
SWITCH to aspirate Beads and run a count cycle.
5. Observe the mean channel values for the 90o and 90o
depolarized channels, and refer to Table 5-4. If the
displayed mean channel values are within specifications
and the recorded gain settings are less than 2500, the
dynode voltages need not be adjusted and the procedure
is completed. If either of the above are out-of-range, the
dynode voltage(s) for the channel(s) must be adjusted.
6. Remove the top cover from the analyzer, and locate the
PMT preamplifiers on the right side of the optical bench.
The board on the left is for the 90o channel and the board
on the right is for the 90o depolarized channel. R11 adjusts
the dynode voltage on both boards.
7. Press
MORE

a. Place 2 mL of diluent in a clean container.

to first Diagnostics Screen, and press

b. Add 15 drops of 7.00 beads and mix well.

F12
F1
SET POINT ENTRY

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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8#0#9HULILFDWLRQ#3URFHGXUHV VP-23 WBC Gain Verification/Adjustment


8. Move cursor to WBC 90D, enter 2000, and press
ENTER. Move cursor to WBC 90DP, enter 2000, and
press ENTER.

14. Press
DIAGNOSTICS
MORE

9. Press
SET ANALYZER
RETURN
RETURN
MORE TO
WBC DATA
10. Cover optical bench with black cloth to block ambient light.
11. Place the 7.00 beads solution under the probe, and
press
EXTENDED WBC COUNT
START
to aspirate the Beads and start the cycle.
o

NOTE: Both 90 channels are extremely sensitive to


ambient light. The black cloth must remain in place when
adjusting the dynode voltages.
12. Observe mean channel value on right display and adjust
R11 on 90o preamplifier (left PCB) until the displayed
values approximate the specifications shown in Table 5-1.
(Note that this is a coarse preliminary manual adjustment
that will be more finely tuned with the auto gain circuitry.)
13. Repeat steps 11 and 12 for the 90o depolarized
preamplifier (right PCB).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

VOLTAGE READINGS
15. Verify that both dynode voltages are less than 9.00 (900
volts). Dynode voltages greater that 900 volts indicate a
problem, and the problem must be isolated and corrected
before continuing.

VP-23 WBC Gain Verification/Adjustment


The following procedure verifies, and if necessary, adjusts the
gain of the WBC channels using 7.00 beads as a reference
material.
There are two modes available for adjusting the WOC gain ENTER SETTING and AUTO GAIN ADJUSTMENT.
In the ENTER SETTING mode the proper gain setting is
calculated manually and then entered. Use the following
equation when using this mode.
New Gain Setting =

Desired Mean Channel


Present Mean Channel

X Present Setting

In the AUTO GAIN ADJUSTMENT mode Beads is run on the


analyzer and the new gain setting is calculated and entered
automatically. We will use this mode, if necessary, in the
following procedure.

Required Tools & Materials:

7.00 Diameter Beads

CELL-DYN 3200 Diluent/Sheath

Part Number 9140300 August 1998

5 - 23

8#0#9HULILFDWLRQ#3URFHGXUHV VP-24 WBC OPTI-CAL Verification/Adjustment

Clean Container

Verification Procedure:
1. Prepare the WBC Beads solution:
a. Place 2mL of diluent in a clean container.
b. Add 15 drops of 7.00 beads and mix well.
2. From Main Menu, press
DIAGNOSTICS
MORE
GAIN ADJUSTMNTS
3. Press
VERIFY GAINS
WBC GAINS
4. If necessary, change the Mean Channel Numbers to the
desired values (Table 5-1) and press
ENTER
5. Place Beads solution under probe, press
START
and press the START SWITCH to aspirate Beads and
run a count cycle.
6. If the Mean Channel Numbers are within specifications,
skip steps 7 through 12.
7. Press
RETURN
to access AUTO GAIN ADJUSTMNT

CELL-DYN 3200 SYSTEM SERVICE MANUAL

8. Press
AUTO GAIN ADJUSTMNT
WBC GAINS
9. If necessary, change the Mean Channel Numbers to the
desired values (Table 5-1) and press ENTER.
NOTE: You must run Beads twice in order to set gains.
The first run is needed to calculate new settings, and the
second is to verify the settings.
10. Place Beads solution under probe, press
START
and press the START SWITCH to aspirate Beads and
run a count cycle.
11. Place Beads solution under probe and press START
SWITCH to aspirate Beads and run a count cycle to
verify.
12. Press
Y
ENTER
to save new gain settings.

VP-24 WBC OPTI-CAL Verification/Adjustment


The following procedure verifies, and if necessary, adjusts the
gain of the WBC 90o and 90oD channels using OPTI-CAL as a
reference material. It also determines and stores the Beads
reference channels to be used for reference after an OPTI-CAL
gain adjustment.

Part Number 9140300 August 1998

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8#0#9HULILFDWLRQ#3URFHGXUHV VP-24 WBC OPTI-CAL Verification/Adjustment


Required Tools & Materials:

9. Press

7.00 Diameter Beads, PN 1606004

CELL-DYN 3200 Diluent/Sheath

Clean Container

10. Change window settings to conform to the OPTI-CAL


assay sheet.

OPTI-CAL

11. Press

CELL REGION

RETURN

Verification/Adjustment Procedure:

12. Toggle the BLOOD/BEADS key to blood.

1. Insure Channels 1 through 4 offset voltages are less than


2 volts.

13. Run the OPTI-CAL 3 times and record the mean channel
values for GRANs on 90o and 90oD on each run.

2. Insure Laser power is greater than 5mw and less than


7mw.

14. Compute the average mean channel for 90o and 90oD for
the three runs.

3. Insure Channel WOC gain settings are less than 2500.

15. Compare the average to the Gran mean channel location


given in the OPTI-CAL assay sheet. If it is within
specifications, skip steps 16 through 20.

4. Insure Dynode voltages are less than 900 volts.


5. Insure the CVs on all 4 channels are: 0o < 3.5%, 10o <
3.5%, 90o < 12.5%, 90oD < 12.5%.
6. Insure the Auto Gain function has been run and the gains
have been set to the following:
0o = 35 +/- 3, 10o = 65 +/- 5.
7. From Main Menu, press

16. Calculate the new gain values for 90o and 90oD, use the
following equation:
X (Y/Z)
Where:
X = Current Gain Value.

DIAGNOSTICS

Y = Mean Channel Value from Assay Sheet.

F12

Z = Current Mean Channel from Step 14.

F1
8. Press
BEADS/BLOOD COMPARE

CELL-DYN 3200 SYSTEM SERVICE MANUAL

17. Press
RETURN
SET POINT ENTRY

Part Number 9140300 August 1998

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8#0#9HULILFDWLRQ#3URFHGXUHV VP-25 NOC Gain Adjustment


18. Enter the new value for 90o gain and press

26. Press
MORE

ENTER

GAIN ADJUSTMNTS

19. Enter the new value for 90oD gain and press
ENTER

27. Press

SET ANALYZER

AUTO GAIN ADJUSTMNT

RETURN

WBC GAINS

20. Press
BEADS/BLOOD COMPARE
and repeat steps 12 through 15.
21. Toggle BLOOD/BEADS key to BEADS.
22. Prepare the Bead solution.
a. Place 2mL of diluent in a clean container.
b. Add 15 drops of 7.00 beads and mix well.
23. Run the Beads three times and record the Mean Channel
for 90o and 90oD on each run. Verify that 0o and 10o Mean
Channels are 35+/-3 and 65+/-5 respectively and all CVs
are within specification on each run.
24. Compute the average Mean Beads Channel for 90o and
90oD for the three runs.
NOTE: These Beads Channel locations are now specific
to this analyzer and must be entered into the AUTO GAIN
table.

28. Enter the Beads Channel numbers for 90o and 90oD.
29. Run an Auto Gain using Beads to enter these channel
locations into the default table.
30. Reboot system on a Setup Disk. Perform a GET to store
these values.

VP-25 NOC Gain Adjustment


The NOC gains are not calibrated with beads because the 0o
and 10o channels are used for both WOC and NOC counting.
However, the NOC gains must be higher than those used for
WOC by a factor of 1.5. In this procedure, we simply multiply
the WOC 0o and 10o gains by 1.5 and change the NOC 0o and
10o setpoints to the new values.

Required Tools & Materials:

None

25. Press
RETURN
RETURN

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 26

8#0#9HULILFDWLRQ#3URFHGXUHV VP-26 RBC/PLT 0 Gain Verification/Adjustment


Verification/Adjustment Procedure:
1. From Main Menu, press
DIAGNOSTICS
F12
F1
2. Press
SET POINT ENTRY

VP-26 RBC/PLT 0 Gain


Verification/Adjustment
The following procedure verifies and, if necessary, adjusts the
gain of the RBC/PLT 0o channel using Beads spheres as a
reference material.

Required Tools & Materials

7.00 Diameter Beads, PN 1606004

3. Move the cursor to the NOC 0o setpoint.

CELL-DYN 3200 Diluent/Sheath

4. Multiply the displayed WOC 0o setpoint by 1.5, change the


NOC 0o setpoint to the new calculated value, and press

Red-Top Vacutainer

Transfer Pipette

ENTER
5. Move the cursor to the NOC 10o setpoint.
6. Multiply the displayed WOC 10o setpoint by 1.5, change
the NOC 10o setpoint to the new calculated value, and
press
ENTER
7. If necessary, move the cursor to the NOC Lo threshold,
change it to 400, and press
ENTER
8. Press
SET ANALYZER
to store the new values.
9. Press
RETURN

Verification/Adjustment Procedure:
1. Prepare the 7.00 beads solution:
a. Mix the 7.00 beads by shaking vigorously.
b. Add 15 drops of 7.00 beads to a vacutainer.
c. Add 2 ml of 3200 Diluent/Sheath reagent.
2. From Main Menu, press
DIAGNOSTICS
MORE
GAIN ADJUSTMNTS
3. Press
VERIFY GAINS
RBC/PLT 0O GAIN

RETURN
CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 27

8#0#9HULILFDWLRQ#3URFHGXUHV VP-27 RBC/PLT 10 Gain Verification/Adjustment


4. If necessary, change the Mean Channel Number to the
desired value and press
ENTER
5. Place Beads solution under probe, press

11. Place Beads solution under probe and press START


SWITCH to aspirate Beads and run a count cycle to
verify.
12. Press

START

and press the START SWITCH to aspirate Beads and


run a count cycle.

to save new gain setting.

6. If the Mean Channel Number is within specifications (180


+/- 1), skip steps 7 through 12.
7. Press
RETURN
to access AUTO GAIN ADJUSTMNT.

ENTER

VP-27 RBC/PLT 10 Gain


Verification/Adjustment
The following procedure verifies and, if necessary, adjusts the
gain of the RBC/PLT 10o channel using Beads spheres as a
reference material.

8. Press
AUTO GAIN ADJUSTMNT
RBC/PLT 0O GAIN
9. If necessary, change the Mean Channel Number to the
desired value and press
ENTER
NOTE: You must run Beads twice in order to set gain. The
first run is needed to calculate new setting, and the second
is to verify the setting.
10. Place Beads solution under probe, press
START
and press the START SWITCH to aspirate Beads and
run a count cycle.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Required Tools & Materials:

3.29 or 3.31 Beads, PN 1606005

CELL-DYN 3200 Diluent/Sheath

Red-Top Vacutainer

Transfer Pipette

Verification/Adjustment Procedure:
1. Prepare the 3.29 or 3.31 Beads solution.
a. Mix the Beads by shaking vigorously.
b. Add 10 drops of Beads and 20 drops of diluent to a
vacutainer and mix well.

Part Number 9140300 August 1998

5 - 28

8#0#9HULILFDWLRQ#3URFHGXUHV VP-28 MAM 0 & 10 Log Amplifier Verification


2. From Main Menu, press
DIAGNOSTICS
MORE
GAIN ADJUSTMNTS
3. Press
VERIFY GAINS
RBC/PLT 10O GAIN
4. If necessary, change the Mean Channel Number to the
desired value and press
ENTER
5. Place Beads solution under probe, press

NOTE: You must run Beads twice in order to set gain. The
first run is needed to calculate new setting, and the second
is to verify the setting.
10. Place Beads solution under probe, press
START
and press the START SWITCH to aspirate Beads and
run a count cycle.
11. Place Beads solution under probe and press START
SWITCH to aspirate Beads and run a count cycle to
verify.
12. Press
Y

START

ENTER

and press the START SWITCH to aspirate Beads and


run a count cycle.

to save new gain setting.

6. If the Mean Channel Number is within specifications


channel (120 +/- 1 for 3.29 or 121+/- 1 for 3.31), skip steps
7 through 12.
7. Press
RETURN
to access AUTO GAIN ADJUSTMNT.
8. Press
AUTO GAIN ADJUSTMNT
RBC/PLT 10O GAIN.
9. If necessary, change the Mean Channel Number to the
desired value and press

VP-28 MAM 0 & 10 Log Amplifier Verification


The following procedure verifies the dynamic range of the 0o
and 10o log amplifiers on the MAM by running the 3 Beads and
calculating the ratio of the peak channels of the three sizes.
The CELL-DYN 3200 uses the 0o channel for and MCV, and the
10o channel for RBCs, PLTs, and MPV, with the log amplifiers
used for both outputs. The 0o channel gain is set with 7.00
beads by placing the peak channel in 180 +/- 1, and the lower
range is verified with a ratio to a 4.99 RBC 0o Bead peak
channel number. The 100 gain is set with 3.31 or 3.29 Beads by
placing the 3.29 peak channel in 120 +/- 1 or the 3.31 peak
channel in 121 +/- 1, and the upper range is verified with a ratio
to a 7.00 beads RBC 10o peak channel number.

ENTER
CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 29

8#0#9HULILFDWLRQ#3URFHGXUHV VP-28 MAM 0 & 10 Log Amplifier Verification


The following table and equations are an example of a typical
RBC and PLT verification/adjustment.
Table 5-5

Transfer Pipette

Verification/Adjustment Procedure:

0o & 10o Log Amplifier Verification

Beads

RBC 0o
P.C.

RBC 10o
P.C.

PLT 0o P.C.

PLT 10o P.C.

3.29

N/A

N/A

N/A

Set at 120

4.99

Falls at
129

N/A

N/A

N/A

7.00

Set at
180

Falls at 200

N/A

N/A

1. Prepare the 7.00 beads solution:


a. Mix the 7.00 beads by shaking vigorously.
b. Add 15 drops of 7.00 beads to a vacutainer.
c. Add 2 ml of 3200 Diluent/Sheath reagent.
2. In the RUN menu, press
SPECIMEN TYPE
BEADS
RETURN.

0o Equation:
7.00 RBC 0o P.C.
4.99 RBC 0o P.C.

3. From Main Menu, press


= 1.400.04

180 = 1.39 (within spec)


129

DIAGNOSTICS
MORE
RBC DATA

10 Equation:
7.00 RBC 10o P.C. = 1.670.04
3.xx PLT 10o P.C.

200 = 1.66 (within spec)


120

Required Tools & Materials:

7.00 Diameter Beads, PN 1606004

3.29 or 3.31 Beads, PN 1606005

4.99 Beads, PN 1606003

CELL-DYN 3200 Diluent/Sheath

Red-Top Vacutainer

CELL-DYN 3200 SYSTEM SERVICE MANUAL

4. Turn smoothing off, place Beads solution under probe,


and press the START SWITCH to aspirate Beads and
run a count cycle.
5. When cycle is completed, view RBC 0 DATA and RBC 0
HISTOGRAM, and verify the peak is in channel 180 +/- 1
channel. If the RBC 0o peak channel number is within
specifications, record the RBC 0o and RBC 10o peak
channel numbers, and skip step 6.
6. Perform RBC/PLT 0o Gain Verification/Adjustment
(VP-26), and repeat steps 3 through 5.
7. Prepare the 4.99 beads solution:
a. Mix the 4.99 beads by shaking vigorously.
Part Number 9140300 August 1998

5 - 30

8#0#9HULILFDWLRQ#3URFHGXUHV VP-28 MAM 0 & 10 Log Amplifier Verification


b. Add 15 drops of 4.99 beads to a vacutainer.
8. Place the Beads under probe, and press the START
SWITCH to aspirate Beads and run a count cycle.
9. When cycle is completed, view RBC 0 DATA and RBC 0
HISTOGRAM and record the RBC 0o peak channel
number
10. Prepare the 3.29 or 3.31 Beads solution:
a. Mix the Beads by shaking vigorously.
b. Add 10 drops of Beads and 20 drops of diluent to a
vacutainer and mix well.
11. Press
DIAGNOSTICS
PLT DATA
PLT 10 DATA.
12. Turn smoothing off, place Beads solution under probe,
and press the START SWITCH to aspirate Beads and
run a count cycle.

15. Plug the appropriate 0o peak channel numbers into the


following equation, and calculate the 0o log amplifier ratio.
If the ratio is out-of-specifications, the 0o log amplifiers
must be adjusted (VP-29) or the MAM must be replaced.:
0o Equation:
7.00 RBC 0o P.C. = 1.40 0.04
4.99 RBC 0o P.C.
16. Plug the appropriate 10o peak channel numbers into the
following equation, and calculate the 10o log amplifier
ratio. If the ratio is out-of-specifications, the 10o log
amplifiers must be adjusted (VP-30) or the MAM must be
replaced.

10o Equation:
7.00 RBC10o P.C. = 1.67 0.04
3.xx PLT10o P.C.

13. When cycle is completed, view PLT 10 DATA and PLT


10 HISTOGRAM, and verify the peak is in channel (120
+/- 1 for 3.29 or 121+/- 1 for 3.31). If the PLT 10o peak
channel number is within specifications, record the PLT 10
peak channel number, and skip step 14.
14. Perform RBC/PLT 10o Gain Verification/Adjustment
(VP-27), and repeat steps 12 and 13.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 31

8#0#9HULILFDWLRQ#3URFHGXUHV VP-29 0 MAM Log Amplifier Verification/Adjustment

VP-29 0 MAM Log Amplifier


Verification/Adjustment
The following procedure verifies and, if necessary, adjusts the
0o log amplifier gains and offsets on the MAM. Refer to Figure
5-6. A voltage-divider is used to inject a 10mv reference voltage
into the 0o log amplifier, and gains and offsets are adjusted to
provide the initial setup of the 0o log amplifier chain.
Figure 5-6

Voltage-Divider Fixture
R ed (+1 5V )
5K O H M S

100 O H M S
3 2 0 80

Required Tools & Materials:

Voltage-Divider Fixture, PN 9450202

Digital Voltmeter

Potentiometer Adjustment Tool or Small Slotted


Screwdriver

Verification/Adjustment Procedure:
1. Refer to Figure 5-6. Install the fixture on the MAM by
attaching the red lead to the top of L2, black lead to TP7,
and the yellow lead to TP8.
2. Attach the positive lead of the DVM to TP8 and the
negative lead to TP7.

Yellow (+10m v)

3. Adjust the pot on the fixture for a reading of +10.0mv +/0.2mv.

B lack (G N D )

4. Move the positive lead to TP11, and adjust R102 for


-23.0mv +/- 0.2mv

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 32

8#0#9HULILFDWLRQ#3URFHGXUHV VP-29 0 MAM Log Amplifier Verification/Adjustment


Figure 5-7

MAM Test Points & Potentiometers

TP 2
R84
3 2 0 81

R102

TP 11

R106
TP 9

TP 7
R137

TP 8

TP 1 7
R132

TP 1 5

L2
TP 1 8

R138

TP 2 1

R131

TP 1 6

5. Move the positive lead to TP15, and adjust R132 for


-1.000V +/- 0.010V.
6. Move the positive lead to TP16, and adjust R137 for 3.68V
+/- 0.010V.
7. Leave meter as is, and adjust R132 for a reading of 1.72V
+/- 0.010V.
NOTE: After performing this procedure the 0o gain must
be verified/adjusted with Beads (VP-26).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 33

8#0#9HULILFDWLRQ#3URFHGXUHV VP-30 10 MAM Log Amplifier Verification/Adjustment

VP-30 10 MAM Log Amplifier


Verification/Adjustment
The following procedure verifies and, if necessary, adjusts the
10o log amplifier gains and offsets on the MAM. Refer to Figure
5-8. A voltage-divider is used to inject a 10mv reference voltage
into the 0o log amplifier, and gains and offsets are adjusted to
provide the initial setup of the 10o log amplifier chain.
Figure 5-8

Voltage-Divider Fixture
R ed (+1 5V )
5K O H M S

100 O H M S
3 2 0 80

3. Adjust the pot on the fixture for a reading of +10.0mv +/0.2mv.


4. Move the positive lead to TP18, and adjust R106 for
-10.0mv +/- 0.2mv
5. Move the positive lead to TP21, and adjust R131 for
-1.000V +/- 0.010V.
6. Move the positive lead to TP9, and adjust R138 for 4.20V
+/- 0.010V.
7. Leave meter as is, and adjust R131 for a reading of
0.750V +/- 0.010V.
NOTE: After performing this procedure the 10o gain must
be verified/adjusted with Beads (VP-27).

Yellow (+10m v)

B lack (G N D )

Required Tools & Materials:

Voltage-Divider Fixture, PN 9450202

Digital Voltmeter

Potentiometer Adjustment Tool or Small Slotted


Screwdriver

Verification/Adjustment Procedure:
1. Refer to Figure 5-6. Install the fixture on the MAM by
attaching the red lead to the top of L2, black lead to TP7,
and the yellow lead to TP17
2. Attach the positive lead of the DVM to TP17 and the
negative lead to TP7.
CELL-DYN 3200 SYSTEM SERVICE MANUAL

VP-31 Aspirate/Vent Needle Verification


Required Tools & Materials:

None

1. From Main Menu, press


DIAGNOSTICS
MORE
TOWER TEST
2. Press
NEEDLE DOWN
3. Verify that the needle moves down smoothly without any
stopping or jerking. Also verify that the GS1 sensor status
displays the following:
GS1 UP:SENSOR STATUS
Part Number 9140300 August 1998

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8#0#9HULILFDWLRQ#3URFHGXUHV VP-32 GS2 Stop Solenoid Verification


GS1=1

VP-33 Bar Code Spin Assembly Verification

GS1 DOWN:SENSOR STATUS


GS1=0
4. Press
NEEDLE UP
5. Verify that the needle moves up smoothly without any
stopping or jerking.

VP-32 GS2 Stop Solenoid Verification


Required Tools & Materials:

None

Verification/Adjustment Procedure:
1. From Main Menu, press

Required Tools & Materials:

None

Verification/Adjustment Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
TOWER TEST
2. Place a tube at the read station, and press
SPIN MOTOR
3. Verify that the tube is spun smoothly without any stopping
or jerking.
4. Press

DIAGNOSTICS

STOP MOTOR

MORE

to return to DIAGNOSTICS.

TOWER TEST
2. Alternately press Retract Solenoid and Extend
Solenoid.

VP-34 Tube Height Sensors (S1/S2)


Verification

3. Verify that the GS1 solenoid retracts and extends


smoothly and fully with each command. Also verify that
LED DS1 on SHM1 is on with the retract command and off
with the extend command.

This diagnostic routine first moves GS1 and GS2 down and
then up in the same manner as a count cycle. It then displays
the status of sensors GS1, S1, and S2 in both the up and down
positions (1=sensor blocked, 0=sensor unblocked). It also
displays the type of tube sensed at the aspirate position: BD,
SST, or No Tube.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 35

8#0#9HULILFDWLRQ#3URFHGXUHV VP-35 Mixer Up/Down Verification


Required Tools & Materials:

BD Specimen Tube

Sarstedt Specimen Tube

Verification/Adjustment Procedure:
1. From Main Menu, press
DIAGNOSTICS

GS1=0 S1=1 S2=1SST

No Tube Example:
GS1 UP: Sensor Status
GS1=1 S1=0 S2=0
GS1 Down: Sensor Status Tube Type
GS1=0 S1=0 S2=1 No Tube

MORE
TOWER TEST
2. Place the appropriate tube at the vent/aspirate position.
3. Press
SENSOR TEST
GS1 DOWN
4. Verify the following sensor status and tube type for each
tube condition.

VP-35 Mixer Up/Down Verification


Required Tools & Materials:

Verification/Adjustment Procedure:
1. From Main Menu, press
DIAGNOSTICS

BD Tube Example:

MORE

GS1 UP: Sensor Status


GS1=1 S1=0 S2=0
GS1 Down: Sensor Status Tube Type
GS1=0 S1=1 S2=0BD

Sarstedt Tube Example:

None

LOADER TEST
2. Alternately press MIXER DOWN and MIXER UP and
verify that the assembly moves up and down smoothly
without any stopping or jerking. Also verify that the home
sensor is 1 when up and 0 when down.

VP-36 Mixer Head Rotation Verification

GS1 UP: Sensor Status


GS1=1 S1=0 S2=0
GS1 Down: Sensor Status Tube Type
CELL-DYN 3200 SYSTEM SERVICE MANUAL

Required Tools & Materials:

None

Part Number 9140300 August 1998

5 - 36

8#0#9HULILFDWLRQ#3URFHGXUHV VP-37 Mixer Bladders Verification


Verification/Adjustment Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
LOADER TEST
2. Alternately press ROTATE MIX UP and ROTATE MIX
DOWN and verify that the mixer head rotates up and
down smoothly without any stopping or jerking. Also verify
that the home sensor is 0 when up and 1 when down.

VP-37 Mixer Bladders Verification


Required Tools & Materials:

None

Verification/Adjustment Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
LOADER TEST
2. Press
CYCLE BLADDERS

VP-38 Rack Advance & Tube Sensors


Verification
Required Tools & Materials:

Specimen Tubes

Specimen Loader Rack

Verification/Adjustment Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
LOADER TEST
2. Place a rack with alternating tubes at the load side rear
wall.
3. Press
START RACK ADVANCE
4. Verify that the rack indexes smoothly through the
processing station without any stopping or jerking during
index movements.
5. Verify that when a tube is present at a sensor position a 1
is indicated, and when a tube is not present a 0 is
indicated.

3. Alternately press GRIP TUBE and RELEASE TUBE


and verify that the mixer bladders inflate and deflate
completely, and that there are no pressure or vacuum
leaks.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 37

8#0#9HULILFDWLRQ#3URFHGXUHV VP-39 Cross Transfer Arms & Rack Sensors Verification

VP-39 Cross Transfer Arms & Rack Sensors


Verification

5. Remove rack from the load side, place five racks in the
unload side, and press
EXTEND ARMS
Verify the following sensor status, and press RETRACT
ARMS.

Required Tools & Materials:

5 Racks

SENSOR STATUS

Verification/Adjustment Procedure:

EXTEND ARMS:LOAD SENSOR = 1

1. From Main Menu, press

UNLOAD FULL

DIAGNOSTICS

UNLOAD WARN = 1

MORE
LOADER TEST
2. Leave the load side and unload side empty, and
alternately press EXTEND ARMS and RETRACT
ARMS.
3. Verify that the arms extend and retract smoothly without
any stopping or jerking.
4. Place one rack in the load side, leave unload side empty,
and press
EXTEND ARMS
Verify the following sensor status, and press
RETRACT ARMS
SENSOR STATUS
EXTEND ARMS:LOAD SENSOR = 0
UNLOAD FULL

=0

=1

VP-40 Rack Bar Code Labels Verification


Required Tools & Materials:

5 Racks

Verification/Adjustment Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
VER RACK BAR CODE
2. Manually move a rack through the processing station,
positioning the rack and tube position bar code labels at
the slot in the processing station.
3. Verify that the bar code number on the screen matches the
number on the rack.

UNLOAD WARN = 0

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 38

8#0#9HULILFDWLRQ#3URFHGXUHV VP-41 Mixer Bladders Pressure Verification/Adjustment

VP-41 Mixer Bladders Pressure


Verification/Adjustment

7. Adjust pressure regulator (just to right of load side empty


sensor) for 6.5 psi +/- 0.2 psi.
8. Reconnect tubing to vacuum/pressure port.

Required Tools & Materials:

Pressure Gauge

Slotted Screwdriver

Verification/Adjustment Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
VOLTAGE READINGS
2. Verify Press 1 psi is 12.5-13.5 psi. If out-of-range,
perform Vacuum & Pressure Level Verification/Adjustment
(VP-16).
3. Press
MORE

VP-42 Shear Valve Driver Lubrication


Procedure
Required Tools & Materials:

Oil PN 14237-015 (Dallas/Europe), 1605806 (Santa Clara)

Verification Procedure:
1. Power off instrument using the proper shutdown
procedure.
2. Remove the shear valve driver from the Analyzer.
3. Lubricate the assembly as shown in Figure 5-9.
4. Re-install the shear valve driver and verify instrument
performance.

LOADER TEST
4. Press
CYCLE BLADDERS
5. Remove tubing from vacuum/pressure port on mixer head,
and connect tubing to pressure gauge.
6. Press
GRIP TUBE
to apply pressure.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 39

8#0#9HULILFDWLRQ#3URFHGXUHV VP-43 Optics Bench Cleaning Procedure


Figure 5-9

Shear Valve Driver Lubrication Points

2-3 D rop s O f O il 3 P lace s

F ront
3 2 0 82

2-3 D rop s O f O il 2 P lace s

VP-43 Optics Bench Cleaning Procedure


'$1*(5=#/$6(5#/,*+7 Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.

Required Tools & Materials:

Cotton Swabs

High Grade Lens Paper (Kodak recommended - DO NOT


USE Baxter S/P, as it may scratch the lens.)

Canned Air - Optics Grade

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Verification Procedure:
1. Clean the left side and rear fan filters.
2. Remove Analyzer top cover and plastic optics covers.
3. Close Laser Shutter.
4. Using pressurized air, remove dust and dirt from optics
bench and surrounding area.

Part Number 9140300 August 1998

5 - 40

8#0#9HULILFDWLRQ#3URFHGXUHV VP-43 Optics Bench Cleaning Procedure


5. Using Kodak lens paper on the end of a cotton swab,
clean rear mirror, cylindrical lens, front mirror, imaging
lens, and flow cell.
&$87,21=##Do not use lens cleaning solution on
mirrors. If cleaning fluid is needed, use reagent grade
methanol only.

9. Check for a red glow or halo around the forward slit. If


laser output lens dirty, a halo will appear around football
shaped laser beam. Refer to Figure 5-11.
Figure 5-11

Forward Slit Halo

F orw ard
S lit
F ootball
S haped
B eam

6. Repeat cleaning process to ensure all residual particles


are removed.
7. Open Laser Shutter.
8. With laser on, check for a red glow or halo around opening
where laser beam exits the laser tube. Refer to Figure
5-10.
Figure 5-10

Laser Output Halo

N O R ed
H alo
3 2 0 84

Laser Tube
R ear
M irror

N O R ed
H alo
3 2 0 83

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 41

8#0#9HULILFDWLRQ#3URFHGXUHV VP-43 Optics Bench Cleaning Procedure


10. If halo exists in either location, clean laser with dry cotton
swab, or use lens paper. Refer to Figure 5-12.
Figure 5-12

Figure 5-13

Mark Mirror Positions


M ark
Location

Laser Tube Cleaning


Laser Tube

R ear
M irror

3 2 0 87

Insert C otton
S w ab H ere

b. Remove screws and mirror. Refer to Figure 5-14.

3 2 0 85

Figure 5-14

Mirror Removal

11. Close Laser Shutter.

Tape

12. Clean 10o mirror.


a. Mark position of mirror and housing with pencil. Refer
to Figure 5-13.

R em ove
M irror

3 2 0 88

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 42

5 - Verification Procedures VP-44 Optical Flow Cell Cleaning & De-Bubble Procedure
c. Clean mirror using reagent grade methanol or
microscope lens cleaning solution (green) and high
grade lens paper. (Kodak recommended - DO NOT
USE Baxter S/P). Ensure surface is clean; use
compressed air to remove any remaining lint.
d. Replace mirror and align marks. DO NOT tighten
screws.
e. Open Laser Shutter.

16. Perform RBC/PLT 0o Gain Verification/Adjustment


(VP-26).
17. Perform RBC/PLT 10o Gain Verification/Adjustment
(VP-27).

VP-44 Optical Flow Cell Cleaning & De-Bubble


Procedure
DANGER: LASER LIGHT Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.

13. Use the following procedure to adjust the mirror for


maximum 10o mean.
a. Prepare the WBC Beads solution by placing 2 mL of
diluent in a clean container, adding 15 drops of 7.00
beads, and mixing well.
b. From Main Menu, press
n
n
n

DIAGNOSTICS
MORE
WBC DATA

c. Place the 7.00 beads solution under the probe, and


press
n
n
n

EXTENDED WBC
COUNT
START

to aspirate the Beads and start the cycle.


d. Observe the 10o mean, and rotate the mirror for the
highest 10o mean reading.
e. Tighten mirror screws ensuring maximum reading is
maintained, and erase pencil marks.

Required Tools & Materials:

20% Bleach or Abbott, ISE Conditioning Solution


LN1360-02

10 mL Syringe

Needle Nosed Pliers

Verification Procedure:
1. Fill syringe with 20% bleach solution or ISE Cleaning
Solution.
2. From Main Menu, press
n SPECIAL PROTOCOLS
n EMPTY/FILL FLOW CELL
n EMPTY FLOW CELL

14. Perform WBC Gain Verification/Adjustment (VP-23).


15. Perform NOC Gain Adjustment (VP-25).
CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 43

8#0#9HULILFDWLRQ#3URFHGXUHV VP-44 Optical Flow Cell Cleaning & De-Bubble Procedure


3. From Main Menu, press

Figure 5-15

Flow Cell Cleaning Plumbing

DIAGNOSTICS
MORE
SOLENOID OPERATION

Flo w C ell D e-Bu b b le


P ro ce s s

4. To open solenoid 56, press


STEP SOLENOID
type 56, and press
ENTER
5. Refer to Figure 5-15. Remove elbow from right side of
solenoid 57, and connect elbow to syringe.
6. Inject bleach or cleaning solution in short strokes to create
turbulent motion in flow cell.
7. Let solution remain is flow cell for three to five minutes.
&$87,21=##Do not allow ISE cleaning solution to remain in
flow cell for over five minutes.

56

57

In je c t 20 %
B le a ch o r
IS E C le an in g
S o lu tio n
& Air a t
Th is P o in t

91
3 2 0 89

8. Disconnect syringe, and connect reconnect elbow to


solenoid 57.
9. Initialize and prime instrument, and run three background
counts.
10. Verify that background counts are within specifications:
WBC 0.10 K/L, RBC 0.02 M/L, and PLT 5 K/L.
11. Refer to Figure 5-15. Verify that there are no large bubbles
in the flow cell. If large bubbles are present, perform
Optical Flow Cell Wetting Procedure (VP-43).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 44

8#0#9HULILFDWLRQ#3URFHGXUHV VP-45 Optical Flow Cell Wetting Procedure

VP-45 Optical Flow Cell Wetting Procedure


'$1*(5=#/$6(5#/,*+7 Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.

Required Tools & Materials:

CN Free HGB/NOC Lyse

10 mL Syringe

6. Inject HGB lyse reagent in short strokes to create turbulent


motion in flow cell.
7. Let lyse remain is flow cell for three to five minutes.
8. Disconnect syringe, and connect reconnect elbow to
solenoid 57.
9. Initialize and prime instrument, and run three background
counts.
10. Verify that background counts are within specifications:
WBC 0.10 K/L, RBC 0.02 M/L, and PLT 5 K/L.

1. Fill syringe with HGB lyse solution.

11. Refer to Figure 5-16. Verify that there are no large bubbles
in the flow cell. If large bubbles are present, repeat this
procedure.

2. From Main Menu, press

Figure 5-16

Verification Procedure:

Flow Cell Bubbles

SPECIAL PROTOCOLS
EMPTY/FILL FLOW CELL
EMPTY FLOW CELL
3. From Main Menu, press
DIAGNOSTICS

To W a ste /
Va cu u m
C e ll
S tre a m
N O L a rg e
A ir B u b b le s

Fe w S m a ll A ir
B ub b le s a re O K

MORE
SOLENOID OPERATION
4. To open solenoid 56, press
STEP SOLENOID
type 56, and press
ENTER
5. Refer to Figure 5-15. Remove elbow from right side of
solenoid 57, and connect elbow to syringe.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

S he a th P rim e
S he a th In
3 2 0 90
S am p le In

Part Number 9140300 August 1998

5 - 45

8#0#9HULILFDWLRQ#3URFHGXUHV VP-46 Laser Power Output Verification

VP-46 Laser Power Output Verification


'$1*(5=#/$6(5#/,*+7 Avoid direct
exposure to laser beam. Refer to Introduction
Section 1.7 for detailed safety information.

Figure 5-17

Laser Output Power Check


Power
M e te r

5 MW

M o u n t C e n te re d
in B e a m Pa th

Required Tools & Materials:

Laser Power Meter

Optical Alignment Tool Kit, PN 8921183801

32091

Verification Procedure:
1. Ensure laser has been on for at least 15 minutes.
2. Perform Optics Bench Cleaning Procedure (VP-43).
3. Refer to Figure 5-17. Check laser power after first mirror:
a. Mount power meter photodiode centered in beam path.
b. Verify that power is 5 mW or greater.
c. If power is less than 5 mW, replace laser tube.

4. Refer to Figure 5-18. Check laser power after forward slit:


a. Mount power meter photodiode centered in beam path
between forward slit and imaging lens.
b. Power meter should read at least 1/5 of that obtained
in step 3.
5. Perform Optical Offsets Verification (VP-47).

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 46

8#0#9HULILFDWLRQ#3URFHGXUHV VP-47 Optical Offsets Verification


Figure 5-18

Laser Power At Forward Slit

3. Verify that CH 1 through CH4 Offsets are less than 1.00


volt.
4. If any are greater than 1.00 volts, perform Optics Bench
Cleaning Procedure (VP-43).

VP-48 Shear Valve Cleaning Procedure


Required Tools & Materials:
P ow e r
M e ter
1 /5 o f P o w e r
o f First M irro r

DI Water

Clean Container

Lint Free Wipes

3 2 0 92

Verification Procedure:
1. From Main Menu, press
SPECIAL PROTOCOLS

VP-47 Optical Offsets Verification


Required Tools & Materials:

Optical Alignment Tool Kit, PN 8921183801

CLEAN/RES SHEAR VAL


CLEAN SHEAR VAL
2. Refer to Figure 5-19. Unscrew shear valve knob.
&$87,21=##Do not drop center ceramic section.

Verification Procedure:
1. From Main Menu, press
DIAGNOSTICS
MORE
VOLTAGE READINGS
2. Ensure top cover is in place, or cover optics bench with
black cloth.
CELL-DYN 3200 SYSTEM SERVICE MANUAL

3. Remove three ceramic sections from shear valve driver


leaving tubing in place, and place center ceramic piece in
a container of DI water.
4. Use damp lint free wipes to clean front and rear surfaces
of front and rear ceramic sections.

Part Number 9140300 August 1998

5 - 47

8#0#9HULILFDWLRQ#3URFHGXUHV VP-49 HGB Flow Cell Cleaning Procedure


5. Clean center mounting holes in front and rear ceramic
sections.
Figure 5-19

Shear Valve Disassembly

VP-49 HGB Flow Cell Cleaning Procedure


This procedure first uses the Auto Clean routine to clean the
flow cell, and if that is unsuccessful a manual procedure is
used.

Loop
Rear
S he a r
Va lv e
B lo ck

C e n te r
Fron t
Loop

M o un tin g G u id e

K no b
R im N o tch
L o ck N otc h

Required Tools & Materials:

CELL-DYN Enzymatic Cleaner

20% Bleach Solution

20 mL Syringe

3 2 0 93

Verification Procedure:
1. From Main Menu, press
6. Clean mounting guide and lock notches in each ceramic
section.

SPECIAL PROTOCOLS

7. Using a flashlight, inspect ports on front and rear ceramic


sections, and ensure no foreign material blocks ports.

AUTO CLEAN

8. Remove center section from DI water, and clean both


sides to remove any blood or foreign material.
9. Wet all surfaces with DI water and assemble as shown in
Figure 5-19. Ensure wide notches are down and rim notch
on center section is to the right.

MORE
Follow the on-screen instructions to perform the
procedure.
2. Run three background counts to purge enzyme cleaner.
3. From Main Menu, press
DIAGNOSTICS

10. Tighten shear valve knob until it stops.

MORE

11. Press

VOLTAGE READINGS

RESTORE SHEAR VAL


12. Run background counts until all are within specifications:
WBC 0.10 K/L, RBC 0.02 M/L, and PLT 5 K/L.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

4. Verify that the HGB OUTPUT voltage is 5.00 volts


+0.2/-0.00 volts. If the voltage is within range, skip steps 5
through 13.
5. Manually open solenoid 93 to drain liquid from flow cell.

Part Number 9140300 August 1998

5 - 48

8#0#9HULILFDWLRQ#3URFHGXUHV VP-50 Open Mode Aspiration Path Cleaning Procedure


6. Prepare and aspirate 20% bleach solution into syringe.
7. Refer to Figure 5-20. Remove tubing from port and
connect syringe to port.
8. Inject bleach solution until it can be seen exiting top port,
and let remain in flow cell for five to ten minutes.

13. Verify that the HGB OUTPUT voltage is 5.00 volts


+0.2/-0.00 volts. If voltage is slightly out-of-range, perform
HGB Current Verification/Adjustment (VP-5).

VP-50 Open Mode Aspiration Path Cleaning


Procedure

9. Manually open solenoid 93 to drain bleach from flow cell.


:$51,1*=#327(17,$/#%,2+$=$5' The
aspiration needle/probe are sharp and potentially
contaminated with infectious materials. Avoid
contact with the tips of the needle/probe.

10. Remove syringe, and connect tubing to port.


11. Run three background counts to purge bleach.
Figure 5-20

HGB Flow Cell Plumbing

Required Tools & Materials:


Inje ct B lea ch
To Here
R em ove Tu bing
& Co nnect Syringe

H GB Flow Ce ll

CELL-DYN Enzymatic Cleaner

Specimen Tubes

Wire Stylet, Gauge #23

Lint Free Tissues

Diluent

Verification Procedure:
3 2 0 94

12. From Main Menu, press

1. In the RUN mode use CHANGE SAMPLER to select


OPEN SAMPLER.
2. Manually open solenoid 12 to purge liquid from plumbing.

DIAGNOSTICS

3. Remove tubing from top of open probe.

MORE

4. Place a tissue under probe, run stylet into the top of probe
until it extends out the bottom, and wipe any material from
stylet. Run up and down several times and remove.

VOLTAGE READINGS

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 49

8#0#9HULILFDWLRQ#3URFHGXUHV VP-51 Closed Mode Aspiration Path Cleaning Procedure


5. Clean outside of probe several times with tissue
dampened with Enzymatic Cleaner, and reconnect tubing
to top.
6. Place a tube containing 4 mL of Enzymatic Cleaner under
probe, manually open solenoid 12, and close when
Enzymatic Cleaner passes through solenoid 12.
7. Let Enzymatic Cleaner remain for five minutes, and
manually open 12 until plumbing is emptied.
8. Place a tube filled with diluent under probe, and open 12
until tube is emptied to flush plumbing.
9. Run background counts until all are within specifications:
WBC 0.10 K/ L, RBC 0.02 M/ L, and PLT 5 K/L.

VP-51 Closed Mode Aspiration Path Cleaning


Procedure

3. Manually open solenoid 12 to purge liquid from plumbing.


4. Place a tube filled with enzyme cleaner at the aspirate
position, and press
NEEDLE DOWN
5. Manually open solenoid 12, and close when enzyme
cleaner passes through solenoid 12.
6. Manually hold solenoid 38 closed, and momentarily open
solenoid 36 to aspirate enzyme cleaner into vent needle.
7. Let enzyme cleaner remain for five minutes, press
NEEDLE UP
and manually open 12 until plumbing is emptied.
8. Initialize and Prime instrument.
9. Run background counts until all are within specifications:
WBC 0.10 K/L, RBC 0.02 M/L, and PLT 5 K/L.

Required Tools & Materials:

CELL-DYN Enzymatic Cleaner

Specimen Tubes

Verification Procedure:
1. In the RUN mode use CHANGE SAMPLER to select
CLOSED SAMPLER.
2. From Main Menu, press
DIAGNOSTICS
MORE
TOWER TEST

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 50

8#0#9HULILFDWLRQ#3URFHGXUHV VP-52 Power-Up CPU/DCM 7-Segment LED Verification

VP-52 Power-Up CPU/DCM 7-Segment LED


Verification

VP-53 Software Installation Procedure


Required Tools & Materials:

Required Tools & Materials:

CELL-DYN System Installation Disks 1 & 2 P.N. 9380590

None

Verification Procedure:
Verification Procedure:
1. Power-up the system and observe the 7-segment LED on
the CPU/DCM. Refer to the following table for status
conditions.
NOTE: After all the tests pass and solenoids energize, 1
and E will alternately flash in the display. This is a normal
condition and does not indicate a problem.
Table 5-6

CPU/DCM 7-Segment LED Status Conditions

1. Turn system power off.


2. Insert disk #1 in the floppy drive.
3. Turn system power on.
4. Follow the on-screen instructions to install software.
5. At the C:\CD3200> prompt type A:INSTALL and press
ENTER
6. When prompted, install disk #2 and follow the on-screen
directions.

Indicates system power-up.

Indicates that the CPU/DCM flash memory failed.

Indicates that the CPU/DCM first 524K of DRAM


failed.

Indicates that the CPU/DCM second 524K of


DRAM failed.

VP-54 Hard Drive Verification

Indicates that the CPU/DCM third 524K of DRAM


failed.

Required Tools & Materials:

Indicates that the CPU/DCM fourth 524K of DRAM


failed.

Indicates that all CPU/DCM memory passed.

7. When installation is complete, remove disk #2 and


power-down and re-boot instrument.

None

Verification Procedure:
1. In the MAIN menu press
F12
F8

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 51

8#0#9HULILFDWLRQ#3URFHGXUHV VP-55 Floppy Drive Verification


2. At the C:\CD3200 prompt, type CHKDSK/F and press

VP-55 Floppy Drive Verification

ENTER

Required Tools & Materials:

3. At the selection prompt type Y and press

ENTER
to run CHKDSK/F.
.

Verification Procedure:
1. In the MAIN menu, press

1 ,2 7 9 ,6 8 8 ,7 0 4 b y te s to ta l d is k sp a c e
1 3 1,0 7 2 b y te s in 2 h id d e n file s

F12

3 ,1 7 8 ,4 9 6 b yte s in 9 7 d ire cto rie s


7 5 0 ,22 3 ,3 6 0 b y te s in 2 ,2 1 5 u s e r file s
5 2 6 ,1 5 5,7 7 6 b y te s a v a ila b le o n d is k

F8
2. At the C:\CD3200 prompt, type A: and press

3 2 , 7 6 8 b y te s in e a c h a llo c a tio n u n it
3 8 ,0 53 to ta l a llo ca tio n u n its o n d is k
16 ,0 5 7 a v a ila b le a llo ca tio n u n its o n d is k
6 5 5 ,3 6 0 to ta l b yte s m e m ory
6 1 8 ,5 6 0 b yte s fre e

Figure 5-21

None

ENTER
3. At the A:\> prompt, type CHKDSK/F and press
ENTER
4. At the selection prompt type Y and press

3 2 0 95

CHKDSK Results, Hard Disk

4. If lost allocation units are reported, type N to delete.


5. The display should closely resemble Figure 5-22. If a line
is added saying xxxx bytes in bad sectors verify that
the number of bytes is less than 1% of the total disk space.
If the number exceeds 1%, the hard drive is suspect and
should probably be replaced.

ENTER
to run CHKDSK/F.The display should closely resemble
Figure 5-22.
5. If there are no indications of a defect, type C: and press
ENTER
6. Then type rll and press
ENTER
to return to the MAIN menu.

6. If there are no indications of a defect, type rll and press


ENTER to return to the MAIN menu.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

5 - 52

8#0#9HULILFDWLRQ#3URFHGXUHV VP-56 Background Counts Verification


Table 5-7
1 ,4 5 7 ,6 6 4 bytes total disk space
1 9 5 ,0 7 2 bytes in 4 user files
1 ,2 6 2 ,5 9 2 bytes available on disk
5 1 2 bytes in each allocation unit
2 ,8 4 7 total allocation units on disk
2 ,4 6 6 available allocation units on disk
6 5 5 ,3 6 0 total bytes memory
6 1 8 ,5 6 0 bytes free
Figure 5-22

Floppy Drive CHKDSK Results

Background Count Specification

WBC
(WOC)

NOC

RBC

HGB

0.10 k/ L 0.10 k/L 0.02 m/L 0.1g/dL

PLT
5.0 k/L

VP-57 System CV Verification


Required Tools & Materials:

2.5 mL Fresh Normal Blood

Verification Procedure:
1. From Main Menu, press

VP-56 Background Counts Verification


Required Tools & Materials:

None

Verification Procedure:
1. From Main Menu, press
RUN
SPECIMEN TYPE
BACKGROUND
2. Press the TOUCH PLATE to run a background count.
3. Verify that the background counts are within specifications
according to the following table.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

RUN
SPECIMEN TYPE
2. Move the cursor to an empty file, and press
QC SPECIMEN
3. Mix the fresh blood thoroughly, and run 10 times in the
open mode and 10 times in the closed mode.
4. Press
MAIN
QUALITY CONTROL
VIEW QC LOG
5. Verify the CVs are within specification according to the
following table.

Part Number 9140300 August 1998

5 - 53

8#0#9HULILFDWLRQ#3URFHGXUHV VP-58 System Calibration Verification/Adjustment


Table 5-8

b. From MAIN MENU press

CV Specifications

WBC
NOC
(WOC)

RBC

HGB

MCV

PLT

MPV

and toggle F2 to CALIBRATOR.

2.7% 2.7% 1.5% 1.0% 1.0% 4.0% 5.0%

c. Enter the reference values from the assay sheet,


pressing [ENTER] after each entry.

VP-58 System Calibration


Verification/Adjustment

d. Press

e. Prepare the calibrator for processing according to the


assay sheet.

CELL-DYN Calibrator

f.

Verification Procedure:

a. Verify reagent type, expiration date, and volumes are


at least 1/2 full.

e. Verify system precision is within specifications (VP-57).


2. Perform Open Mode Auto Calibration using commercial
calibrator only.
NOTE: If any problems are encountered, refer to
Auto-Cal Procedure-Open Mode in Chapter 6 of the
CELL-DYN 3200 Operations Manual for a complete
description of the calibration procedure.

ACCEPT

to enter the new factors for the parameters that need


calibration.

b. Verify calibrators are not expired.

d. Ensure WOC offsets for channels 1 through 4 are less


than 1.00 volt.

Run the calibrator 5 times, mixing well between runs.

g. Press

1. Pre-calibration checks:

c. Ensure background counts are within specifications


(VP-56).

START AUTO-CAL

to ready the instrument for calibration.

Required Tools & Materials:

CALIBRATION
AUTO-CALIBRATE

VP-59 Vacuum Accumulator #1 Rinsing


Procedure
Required Tools & Materials:

Clean >250 mL Container

DI Water

0.125"ID 0.250"OD Tubing, 1 Foot

#2 Phillips Screwdriver

a. Ensure Analyzer in Open Mode.


CELL-DYN 3200 SYSTEM SERVICE MANUAL

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8#0#9HULILFDWLRQ#3URFHGXUHV VP-60 Vacuum Accumulator #2 Rinsing Procedure


Verification Procedure
1. Initialize Analyzer, and remove Left Side Cover.

5. Place tubing connected to position 4 into container, and


allow all DI water to be aspirated into Vacuum
Accumulator #1.

2. Extend Air Supply out of Analyzer (Vacuum/Pressure


Assembly (RR-7)).

6. Remove tubing from position 4, connect original tubing,


and unpinch position 7.

3. Measure 250 mL (1 cup) of DI water into a clean container.

7. Wait at least 5 minutes to remove any saline bridges


between electrodes.

&$87,21=##Do not place more than stated amount of


DI water in container.

8. From Main Menu, press


DIAGNOSTICS

TO
FL O W PA N E L

9P SI

5P SI 12"H g 8"H g 12P SI 12"H g

DRAIN ACCUMULATORS
9. Initialize Analyzer and repeat Step 8.

Required Tools & Materials:

32004

Figure 5-23

MORE

VP-60 Vacuum Accumulator #2 Rinsing


Procedure

12P SI

To S L

To S L

4. Refer to Figure 5-23. Pinch tubing at position 7 of


manifold, remove tubing from position 4, and attach
0.125"ID 0.250"OD tubing to fitting at position 4.

Vacuum/Pressure Manifold

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Clean >250 mL Container

DI Water

0.125"ID 0.250"OD Tubing, 1 Foot

#2 Phillips Screwdriver

Verification Procedure
1. Initialize Analyzer, and remove Left Side Cover.
2. Extend Air Supply out of Analyzer (Vacuum/Pressure
Assembly (RR-7)).

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8#0#9HULILFDWLRQ#3URFHGXUHV VP-61 Hard Drive Formatting

6. Remove tubing from position 5, and connect original


tubing.
7. Wait at least 5 minutes to remove any saline bridges
between electrodes.
8. From Main Menu, press

5P SI 12"H g 8"H g 12P SI 12"H g

TO
FL O W PA N E L

5. Place tubing connected to position 5 into container, and


allow all DI water to be aspirated into Vacuum
Accumulator #2.

9P SI

4. Refer to Figure 5-24. Remove tubing from position 5 of


manifold, and attach 0.125"ID 0.250"OD tubing to fitting at
position 5.

12P SI

To S L

To S L

&$87,21=##Do not place more than stated amount of


DI water in container.

3. Measure 250 mL (1 cup) of DI water into a clean container.

32004

Figure 5-24

Vacuum/Pressure Manifold

VP-61 Hard Drive Formatting

DIAGNOSTICS

Required Tools & Materials:

MORE

CELL-DYN 3200 System Service Disk PN 9380700

IDRAIN ACCUMULATORS
9. Initialize Analyzer and repeat Step 8.

Verification Procedure
1. Perform (VP-64) Backup Procedure .
2. Turn System OFF.
3. Insert Service Disk into Floppy Drive.
4. Turn System ON.
5. At A:\ prompt type CHKDSK/F, then press
ENTER

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8#0#9HULILFDWLRQ#3URFHGXUHV VP-62 DOS Installation


6. If there is any indication that the disk is defective, it should
be formatted.
7. Format the hard drive:

5. Remove Service Disk.

ENTER

b. The display will indicate when the format is complete


8. Perform (VP-65) Restore Setup
1 ,2 7 9 ,6 8 8 ,7 0 4 b y te s to ta l d is k sp a c e
1 3 1,0 7 2 b y te s in 2 h id d e n file s

Required Tools & Materials:

3 ,1 7 8 ,4 9 6 b yte s in 9 7 d ire cto rie s


7 5 0 ,22 3 ,3 6 0 b y te s in 2 ,2 1 5 u s e r file s
5 2 6 ,1 5 5,7 7 6 b y te s a v a ila b le o n d is k

None

Verification Procedure

3 2 , 7 6 8 b y te s in e a c h a llo c a tio n u n it
3 8 ,0 53 to ta l a llo ca tio n u n its o n d is k
16 ,0 5 7 a v a ila b le a llo ca tio n u n its o n d is k

Figure 5-25

CHKDSK/F Results, Hard Disk

6. Turn System OFF, wait 5 seconds, then turn System ON.

VP-63 CS/SL SETUP

6 5 5 ,3 6 0 to ta l b yte s m e m ory
6 1 8 ,5 6 0 b yte s fre e

4. At A:\ prompt type INSTALL, then press


ENTER

a. Type FORMAT C:/S and press

3. Turn System ON.

3 2 0 95

1. Enter Diagnostics Hidden Screen


a. From MAIN MENU, press

DIAGNOSTICS

b. Press

F12
F1

TOGGLE CS/SL to appropriate instrument type.

VP-62 DOS Installation


Required Tools & Materials:
Service Disk PN 9380700

NOTE: Instrument type will display on screen.


c. Press

RETURN

Verification Procedure
1. Turn System OFF.
2. Insert Service Disk into Floppy Drive.
CELL-DYN 3200 SYSTEM SERVICE MANUAL

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8#0#9HULILFDWLRQ#3URFHGXUHV VP-64 Backup Procedure

VP-64 Backup Procedure

4. At A:\ prompt, type R to RESTORE, then press


ENTER

Required Tools & Materials:


CELL-DYN 3200 System Setup Disk PN 9380595

This copies Old Version Setup from Setup Disk to Hard


Drive.
5. Remove Setup Disk.

Verification Procedure

6. Turn System OFF, wait 5 seconds, then turn System ON.

1. Turn System OFF.


2. Insert Setup Disk.
3. Turn System ON.
4. At A:\ prompt, type S to SAVE, then press
ENTER.
This copies Old Version Setup from Hard Drive to Setup
Disk.
5. Remove Setup Disk.
6. Turn System OFF, wait 5 seconds, then turn System ON.

VP-65 Restore Setup


Required Tools & Materials:
CELL-DYN 3200 System Setup Disk PN 9380595

Verification Procedure
1. Turn System OFF.
2. Insert Setup Disk.

VP-66 List Mode Data Collection


Required Tools & Materials:
None

Verification Procedure
1. Collect Data with List Mode ON.
a. From MAIN MENU, Press

DIAGNOSTICS

b. Press,

F12
F1
STORE RAW ON/OFF (To ON)

c. Press,

RETURN

d. From MAIN Menu, press

RUN

e. Run specimens to collect list mode data.

3. Turn System ON.

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8#0#9HULILFDWLRQ#3URFHGXUHV VP-67 Download HSSL Com


f.

Press

MAIN
DIAGNOSTICS

g. Press,

F12
F1

h. DO NOT run analyzer with List Mode ON for an


extended period. Each file = approximately 100kb.
When Hard Drive is filled with Data Lists Fatal Fault
condition will result. Then files must be deleted.
2. Download List Mode Data to Diskette.
a. From MAIN MENU, press

F12
F8

b. Type CD\RAWFILE\(yymmdd-the DATE OF


COLLECTION).
c. Press

ENTER

NOTE: Date format is YYMMDD (Year Month Day).


Example: 960321 = 21 March 1996.
d. Place Formatted 1.44mb diskette in Floppy Disk Drive.
e. Type
COPY C:\RAWFILE\960321\(SEQUENCE#).RAW B:.
This will copy one file of LIST MODE DATA from Data
Station to Diskette in Floppy Drive.
NOTE: Sequence # = Specimen Sequence number in
Data Log.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Example: COPY C:\RAWFILE\960921\8411.RAW B:.


If multiple files need to be downloaded, use wild cards.
Such as sequence # 841?.raw. This will download
8410, 8411, 8412, 8413, etc.
3. If Needed - Delete Data Files from Hard Drive.
a. At C:\ prompt type CD\RAWFILE, and press

ENTER

b. Type DIR, and Press

ENTER
to get listing of all files in RAWFILE directory.
c. Select files to be deleted.
d. Type DEL C:\RAWFILE\(FILE NAME) for each file to
be deleted.
4. Press
RETURN

VP-67 Download HSSL Com


Required Tools & Materials
CELL-DYN 3200 System Installation Disks 1 & 2 P.N. 9380590

Verification Procedure
1. Turn system power OFF.
2. Insert disk #1 in the floppy drive.
3. Turn system power ON.
4. When instructed, insert disk #2.

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8#0#9HULILFDWLRQ#3URFHGXUHV VP-67 Download HSSL Com


5. Follow on-screen instructions to download HSSL Com and
setup CS or SL.
a. Go to the Main Menu and press F12, F8 to get to the
C:\CD3200> prompt.
b. Type HSSL
c. If analyzer is an SL, type 0KL <enter>. SKL should be
displayed in the left column. Type 0K <enter>. 0K
should be displayed in the right column.
d. If analyzer is a CS, type 0KS <enter>. 0KS should be
displayed in the left column. Type 0K <enter>. 0K
should be displayed in the right column.

NOTE: 0 = zero
a. Press F8 to exit to the C:\CD3200> prompt.
b. Turn off analyzer, wait 5 sec, turn on analyzer to
reboot.

CELL-DYN 3200 SYSTEM SERVICE MANUAL

Part Number 9140300 August 1998

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