DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.

)
HEMAT. SPOT NO:- S-01
Q.1. Prepare a neat & clean , thin& slim, well-stained PBF (Peripheral blood film) .
Also focus a WBC with horse-shoe-shaped nucleus and acting as the second line
of defense in immune-system of body.
Q.2. By suitable method (based on the principle of acid-haematin formation) ,
estimate the hematological parameter which is used in diagnosis of anaemia. Also
calculate the MCH (Mean corpuscular hemoglobin) if TRBC (Total RBC count)=5.5
millions/cmm.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-02
Q.1. Prepare a neat & clean , thin& slim, well-stained PBF (Peripheral blood film)
.Also focus an agranulocyte with N:C ratio=7/8:1/8.
Q. 2.Perform a pair of suitable haematological time-tests (by “Cappillary glass
tube method” & “Duke’s method” respectively) which is used to differentiate
between HAEMOPHILIA & PURPURA. Also write a short note on “Haemophilia”.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-03
Q.1. Prepare a neat & clean , thin& slim, well-stained PBF (Peripheral blood film)
.Also focus an “antibody forming leukocyte” with N:C ratio=3/4 : 1/4 .
Q.2. Estimate the blood concentration of the O2 carrying pigment (with Fe+2 as
central metallic ion) by “acid haematin method”. Also calculate MCHC (Mean
corpuscular haemoglobin concentration), if PCV=42%.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-04
Q.1. Prepare a neat & clean , thin& slim, well-stained PBF (Peripheral blood film) .
Also focus a leucocyte with coarse, bluish-colored granules, (which are
overcrowding the neucleus ) & nucleus is bilobed, ‘S’-shaped, dark-blue colored.
Q.2. Perform a pair of suitable haematological time-tests (by “Cappillary glass
tube method” & “Duke’s method” respectively) which is generally prescribed in
Snake-bite & before planed surgery. Also add a short-note on “Purpura”.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-05
Q. 1.Prepare a neat & clean , thin& slim, well-stained PBF (Peripheral blood film)
. Also focus a blood cell called as “BLOOD-MICROPHAGE” , whose differential
count is used in the assessment of activity status of bone-marrow.
Q. 2.Estimate Hb conc. by SAHLI’s Method .Also calculate PCV (Packed Cell
Volume), if MCHC (Mean corpuscular haemoglobin concentration) =35%.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-06
Q.1. Prepare a neat & clean , thin& slim, well-stained PBF (Peripheral blood film) .
Also focus a WBC whose granules are coarse, brick-red colored & notovercrowding the nucleus of the cell &their count decrease in STRESS.
Q.2. Perform the estimation of CT-BT (Clotting time-Bleeding time) by suitable
methods. Also add a short note on “ Physiology of Anticoagulants”.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-07
Q.1. Estimate by SAHLI’s Method the total amount of Hb in 5 Liters of blood .Also
calculate the MCH (Mean corpuscular haemoglobin) ,if PCV (Packed cell volume)=
44% & MCV (Mean corpuscular volume)=88 cubic-micrometer .
Q.2. Prepare a neat & clean , thin& slim, well-stained PBF (Peripheral blood film) .
Then perform a DLC(Differential leucocyte count) by “Tally-Bar method”. Also
calculate the absolute number of those leucocytes whose count increase in
parasitic infestations & allergic conditions; if TLC (Total leucocyte count)
=8000/cmm.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-08
Q.1. Perform a haematological estimation one each for diagnosis of Haemophilia
& Purpura , respectively. Also add a short-note on “Role of platelets, Liver & Vit.K
in clotting mechanism”.
Q.2. Perform TLC (Total Leucocyte count) using suitable diluting fluid. Also
calculate the absolute number of blood microphages; if DLC=N=59%, B=1%,
E=2%, M=9% & L=29%. Also add a short note on ‘ Leukemia’.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-09
Q.1. Estimate Hb conc. by “Acid-haematin Method” . Also calculate the MCHC
(Mean corpuscular haemoglobin concentration) & PCV (Packed cell volume); if
MCH (Mean corpuscular haemoglobin)=31 pg and MCV=93 cubic-micrometer.
Q.2. Perform TRBC (Total RBC count) using suitable diluting fluid. Also calculate
the total amount of Haemoglobin of RBCs present in 1liter of such blood and
volume those RBCs present ; if MCV=94.5 cubic-micrometer and MCHC=31.5%.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-10
Q.1. Perform Clotting-time test (by method involving the combination of extrinsic
& intrinsic clotting pathways) and Bleeding-time test (by “Duke’s method”) . Also
add a short-note on “ Physiology of Bleeding-disorders”.
Q.2. Prepare a neat & clean , thin& slim, well-stained PBF (Peripheral blood film) .
Then perform a DLC(Differential leucocyte count) by “Tally-Bar method”. Also
calculate the absolute number of blood cells which are generally useful in
assessing the activity of bone-marrow; if TLC(Total leucocyte count)=6500/cmm.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-11
Q.1. Estimate Hb conc. by SAHLI’s Method of given blood sample . Also calculate
the total amount of Hb (in grams) present in 100 average sized RBCs of this
same blood; if TRBC=5.5 millions/cmm.
Q.2. Perform TLC (Total leucocyte count) using suitable diluting fluid. Also
calculate the absolute number of uni-lobed mature phagocytic blood cells; if
DLC(Differential leucocyte count)=N=55%,E=1%,B=2%, L=34% &M=8%. Also add
a short-note on ‘Leucopenia’.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-12
Q.1. Perform CT-BT by “Capillary glass tube method” and “Duke’s method” ,
respectively. Also add a short-note on “ Physiology of Haemolytic system”.
Q.2. Perform TRBC (Total RBCs count) using suitable diluting fluid. Also calculate
the total volume of 500 such average sized RBCs present in the same blood ; if
MCH (Mean corpuscular haemoglobin) =29pg , & MCHC (Mean corpuscular
haemoglobin concentration) =32%.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-13
Q.1. Estimate Hb conc. by a method based on the formation of acid-haematin .If
in any other blood sample with Hb conc.=12gm% ,TRBC=3.5millions/cmm &
PCV= 35%. ; then by “Wintrobe’s -system of amaemia classification” , what type
of anaemia can be concluded in this case? Also calculate the values of the
required blood standards for this conclusive diagnosis.
Q.2. Prepare a neat & clean , thin& slim, well-stained PBF (Peripheral blood film) .
Also focus a large blood cell, also called as an immediate pre-cursor of tissuemacrophages.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-14
Q.1. Using suitable laboratory methods perform a pair of haematological-time
tests , which are generally prescribed before tooth-extractions & in snake-bite
cases. Also add a short note on “Physiology of Clotting factors”.
Q.2. Prepare a neat & clean , thin& slim, well-stained PBF (Peripheral blood film) .
Also focus a blood cell without any nucleus & with an irregular cell boundary;
and playing a very vital role in haemostasis.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-15
Q.1. Estimate Hb conc. by SAHLI’s method in given blood blood sample.
Calculate the total amount of Hb in the all of the RBCs present in 20 ml of such
blood. Also calculate MCHC (Mean corpuscular hemoglobin concentration) ; if
TRBC =5.6 millions/cmm & PCV (Packed cell volume)=42%.
Q.2. Prepare a neat & clean , thin& slim, well-stained PBF (Peripheral blood film).
Also focus an agrnulocyte with indented nucleus whose count decrease in HIVinfection

DEPARTMENT OF PHYSIOLOGY DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-16
Q.1. Perform CT-BT (Clotting time –Bleeding time ) by appropriate laboratory
methods . Name out the clotting factors & pathways involved. Also add a shortnote on “ The 3rd step of Haemostasis”.
Q.2. Prepare a neat & clean , thin& slim, well-stained PBF (Peripheral blood film).
Also focus a blood cell with fine-dust like & liliac colored granules and also useful
in distinguishing between the blood of two genders (male & female).

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-17
Q.1. Estimate the Hb conc. by SAHLI’s Method in the given blood sample . If the
total amount of Hb in 1000 such equal average sized RBCs =31 ng
(ng=nanograms) and MCV=93 cumic-micrometer. Then calculate the TRBC & PCV
in thia case.
Q.2. Prepare a neat & clean , thin& slim, well-stained PBF (Peripheral blood film).
Also focus a blood cell with bilobed nucleus & coarse granules and these
granules are rich in ECF-A (Eosinophilic chemotactic factor of anaphylaxis) &5-HT.
The count of these cells increase in influenza & chicken-pox viral infections.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-18
Q.1. Perform Clotting Time-Bleeding Time (with the method for CT involving both
the clotting pathways and the method for BT should be without the use of
Sphygmomanometer). Also add a short-note on “ Other haematological
laboratory time- tests for diagnosing bleeding & clotting disorders”.
Q.2. Prepare a neat & clean , thin& slim, well-stained PBF (Peripheral blood film).
Also focus the blood cell whose count is found increased in “Tropical climatic
regions” .

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-19
Q.1. Perform TLC (Total leucocyte count) using suitable diluting solutions .
Calculate the absolute number of those blood cells which are with “abundant &
pale-blue colored cytoplasm” and their count generally increase in Kala-azar&
malaria. ;if DLC=N=55%, E=3%, B=2%, L=30% & M=10%. Also & write a short-note
on “Leucocytosis”
Q.2. Estimate Hb conc. by SAHLI’s method in given blood blood sample.
Calculate MCHC (Mean corpuscular haemoglobin concentration) & MCV (Mean
corpuscular volume) ; if total number of RBCs in 100cmm of blood is
=600millions. And total volume of 10 such RBCs=930cubic-micrometer.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-20
Q.1 Perform TRBC (Total RBCs count) using suitable diluting fluid. In any other
subject with MCHC=35%, & MCH=25pg ;what type of anaemia can be
conclusively diagnosed by “Wintrobes method of Anaemia classification”? Also
calculate the required blood standard for this diagnostic conclusion.
Q.2. Perform CT-BT (Clotting time-Bleeding time) by appropriate laboratory
methods. Also add a short-note on “ Physiology of Clotting pathways”.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-21
Q.1. Prepare a neat & clean , thin& slim, well-stained PBF (Peripheral blood film).
Then perform DLC (Differential leucocyte count) by “Tally-Bar method”. Also
calculate the absolute number of “3-lobed-nucleated phagocytes”; if the TLC
(Total leucocyte count)=7500/cmm.
Q.2. Estimate Hb conc. by suitable haematological method (based on the principle
of Acid-haematin ). Also calculate the total amount of Hb in gm & total number of
RBCs present in 1ml of blood ; If MCV =92cubic-micrometer & MCHC =35%.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-22
Q.1.Prepare a well-stained, thin & slim PBF(Peripheral Blood Film). Focus the
blood cell whose N/C Ratio is 7:1.
Q.2.Perform Hb Estimation in given blood sample by SAHLI’S Method.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-23
Q.1 .Prepare a well-stained, thin & slim PBF(Peripheral Blood Film).Focus the
blood cell whose count increase in BRONCHIAL ASTHMA.
Q.2.Estimate the ABO-Rh Blood Group in given blood sample.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-24
Q.1 .Prepare a well-stained, thin & slim PBF(Peripheral Blood Film).Focus the
blood cell whose N/C Ratio is 3:1.
Q.2. Perform estimation of one hematological parameter each for diagnosing
the HAEMOPHILIA & PURPURA.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-25
Q.1 .Prepare a well-stained, thin & slim PBF(Peripheral Blood Film).Focus the
blood cell whose GRANULES are FINE DUST like & LILIAC in color.
Q.2.Perform one hematological test with CRYSTALS formation ,which are
having MEDICO-LEGAL value.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-26
Q.1 .Prepare a well-stained, thin & slim PBF(Peripheral Blood Film).Focus the
blood cell whose GRANULES are COARSE in texture , BRICK RED in color & NOT
OVERCROWDING the nucleus in the cell.
Q.2.Estimate the ABO-Rh Blood Group in given blood sample.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-27
Q.1 .Prepare a well-stained, thin & slim PBF(Peripheral Blood Film).Focus the
blood cell whose nucleus is KIDNEY shaped or HORSE- SHOE shaped.
Q.2.Perform one hematological test with CRYSTALS formation ,which are
having MEDICO-LEGAL value.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-28
Q.1 .Prepare a well-stained, thin & slim PBF(Peripheral Blood Film). Calculate
the percentage distribution of various type of WBCs by “TALLY BAR METHOD”.
Q.2.Estimate Clotting time & Bleeding time of your own blood by “CAPILLARY
GLASS TUBE” method & DUKE’S method respectively.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-29
Q.1.Estimate the TLC (Total Leucocyte Count) of given blood sample.
Q.2.Estimate Clotting time & Bleeding time of your own blood by “CAPILLARY
GLASS TUBE” method & DUKE’S method respectively.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-30
Q.1.Prepare a well-stained, thin & slim PBF(Peripheral Blood Film).Perform a
test by which we can assess the ACTIVITY of BONE MARROW.
Q.2.Perform one hematological test with CRYSTALS formation ,which are
having MEDICO-LEGAL value.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-31
Q.1.Estimate the TEC (Total Erythrocyte Count) of given blood sample.
Q.2.Perform Hb Estimation in given blood sample by SAHLI’S Method. Also
calculate the value of MCH(Mean Corpuscular Haemoglobin).

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-32
Q.1.Estimate the TEC (Total Erythrocyte Count) of given blood sample.
Q.2.Calculate theMCHC (Mean Corpuscular Haemoglobin Concetration) and
MCV (Mean Corpuscular Volume) ,if the PCV=42%.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-33
Q.1 .Prepare a well-stained, thin & slim PBF(Peripheral Blood Film).Focus the
blood cell whose count decrease DENGUE Fever.
Q.2.Calculate the MCV (Mean Corpuscular Volume),and MCHC (Mean
Corpuscular Haemoglobin Concentration) ,if Hb Conc.=14g% and the PCV=42%.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-34
Q.1 .Prepare a well-stained, thin & slim PBF(Peripheral Blood Film).Focus the
blood cell whose count increase in PARASITIC INFESTATION .
Q.2.Performing DLC (Differential Leucocyte Count), calculate the absolute
number of above referred cells if the TLC (Total Leucocyte Count)=8000/cmm.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-35
Q.1.Prepare a well-stained, thin & slim PBF(Peripheral Blood Film). Focus the
blood cell whose N/C Ratio is 7:1 and are responsible for HUMORAL IMMUNITY.
Q.2.Estimate Clotting time & Bleeding time of your own blood by “CAPILLARY
GLASS TUBE” method & DUKE’S method respectively.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-36
Q.1.Estimate the TEC (Total Erythrocyte Count) of given blood sample.
Q.2.Calculate the MCH(Mean Corpuscular Haemoglobin) & MCHC (Mean
Corpuscular Haemoglobin Concentration); If PCV=45% .

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-37
Q.1.Performing DLC (Differential Leucocyte Count), calculate the absolute
number of cells useful in ARNETH’S COUNT, if the TLC (Total Leucocyte
Count)=7500/cmm.
Q.2.Estimate the ABO-Rh Blood Group of the given blood sample.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-38
Q.1.Prepare a well-stained, thin & slim PBF(Peripheral Blood Film). Focus the
blood cell also named as MICROPHAGE.
Q.2.Perform suitable hematological test by which we can estimate the ACTIVITY
OF BONE MARROW.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-39
Q.1 .Prepare a well-stained, thin & slim PBF(Peripheral Blood Film).Focus the
blood cell whose GRANULES are COARSE in texture , BLUEISH-PURPLISH in color
& are OVERCROWDING the nucleus in the cell.
Q.2.Performing DLC (Differential Leucocyte Count), calculate the absolute
number of above referred cells if the TLC (Total Leucocyte Count)=8000/cmm

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-40
Q.1. Estimate Hb Concentration in given blood sample by SAHLI’S Method. Also
calculate MCH( Mean Corpuscular Haemoglobin) and MCHC(Mean Corpuscular
Haemoglobin Concentration) ; if TRBC=5.5millons/cmm & PCV=44%.
Q.2. Estimate the CLOTTING –TIME & BLEEDING -TIME of your own blood by
“CAPILLARY GLASS TUBE “ Method & DUKE’S Method respectively. What
happens to their values in the case of HAEMOPHILIA & PURPURA?

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-41
Q.1. Perform a haematological test , based on the principle of “ACIDHAEMATIN” , and useful in diagnosis of ANAEMIA.
Q.2. Perform the “BLOOD-TYPING” of the given blood sample ,based on the
LANDSTEINER’S LAW, and useful in BLOOD-TRANSFUSION.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C, JODHPUR (RAJ.)
HEMAT. SPOT NO:- S-42
Q.1. Perform a haematological test , based on the principle of “ACIDHAEMATIN” , and which is significant MEDICO -LEGALY.
Q.2 .Prepare a well-stained, thin & slim PBF(Peripheral Blood Film).Focus a
PHAGOCYTIC blood cell with unilobed nucleus.

DEPARTMENT OF PHYSIOLOGY, DR. S.N.M.C. , JODHPUR(RAJ.)
-: EXERCISE-KEY FOR SPOTS IN HEMATOLOGY LAB FOR PRACTICAL EXAM :MBBS-1ST YEAR (BATCH-56)

(Session:- 2014-15)

Dated:- .

.

SEAT
NO.

EXERCISE
(1)

EXERCISE
(2)

S-01
S-02
S-03
S-04
S-05
S-06

Prepare PBF & Focus Monocyte
Prepare PBF & focus small Lymphocyte
Prepare PBF & focus large Lymphocyte
Prepare PBF & focus Basophil
Prepare PBF & focus Neutrophil
Prepare PBF & focus Eosinophil

S-07

Estimate Hb conc. by SAHLI’s Method &
calculate MCH
Perform CT-BT & write a short note on “Role of

Estimate Hb conc.& calculate MCH
Perform CT-BT& write about “Hemophilia”
Estimate Hb conc. & calculate MCHC
Perform CT-BT & write a short note on “Purpura”
Estimate Hb conc. by SAHLI’s Method & PCV
Perform CT-BT & write a short note on
“Anticoagulants”
Prepare PBF & perform DLC ,Abs. Eosinophil no.

S-08

Perform TLC & absuolutre no. of Neutrophils write

S-09
S-10
S-11
S-12
S-13
S-14
S-15
S-16
S-17
S-18
S-19
S-20
S-21
S-22
S-23
S-24
S-25
S-26
S-27
S-28
S-29
S-30
S-31
S-32
S-33
S-34
S-35
S-36
S-37
S-38
S-39
S-40
S-41
S-42

platelets, Liver & Vit.K in clotting mechanism”
Estimate Hb conc. by SAHLI’s Method &
calculate MCHC &PCV
Perform CT-BT & write a short note on
“Bleeding disorders”
Estimate Hb conc. by SAHLI’s Method &
calculate amount of Hb.
Perform CT-BT & write a short note on
“Haemolytic system “
Estimate Hb conc. by SAHLI’s Method &
calculate MCH,MCV, MCHC
Perform CT-BT & write about “Clotting factors”
Estimate Hb conc. by SAHLI’s Method &
calculate amount of Hb &MCHC
Perform CT-BT & write about “3rd step of
Haemostasis”
Hb conc. by SAHLI’s Method and TRBC & PCV
Perform CT-BT & write a short note on “ Other
tests for bleeding & clotting disorders”
Perform TLC & calculate absolute monocyte
count ,write a short note on “Leucocutosis”
Perform TRBC & calculate MCV
Prepare PBF & perform DLC ans absolute no. of
neutrophils
Prepare PBF & focus Small-lymphocyte
Prepare PBF & focus Eosinophil
Prepare PBF & focus Larg- lymphocyte
Prepare PBF & focus Neutrophil
Prepare PBF & focus Eosinophil
Prepare PBF & focus Monocyte
Prepare PBF & Perform DLC
TLC
PBF& Arneth’s count
TEC (TRBC)
TEC (TRBC)
PBF & focus Platelet
Prepare PBF & focus Eosinophil
Prepare PBF & focus Small- (B-lymphocytes)
TEC (TRBC)
DLC , calculate the absolute no. of Neutrophils
Prepare PBF & focus Neutrophil
Prepare PBF & focus Basophil
Estimate Hb conc. by SAHLI’s Method, calculate
MCH & MCHC
Hb estimation
Preparation of Haemin-crystals

a short note on “Leukemia”
Perform TRBC & calculate Hc amount& RBCs
volume
Prepare PBF & perform DLC& absolute Neotrophil
no.
Perform TLC & absolute no. of Monocytes and
write a short note on “Leucopenia”
Perform TRBC & calculate volume of 500 RBCs
Prepare PBF & focus Monocyte
Prepare PBF & focus Platelet
Prepare PBF & focus large Lymphocyte
Prepare PBF & focus neutrophil
Prepare PBF & focus basophil
Prepare PBF & focus Eosinophil
Estimate Hb conc. & calculate MCHC& MCV
Perform CT-BT & write on “Clotting pathways”
Estimate Hb conc. by SAHLI’s Method & calculate
amount of Hb. And no. of RBCs in 1ml blood
Estimate Hb conc. by SAHLI’s Method
ABO-Rh blood group estimation
Estimation of CT-BT
Preparation of Haemin-crystals
ABO-Rh blood group estimation
Preparation of Haemin-crystals
Estimation of CT-BT
Estimation of CT-BT
Preparation of Haemin-crystals
Estimate Hb conc. & calculate MCH
calculate MCV & MCHC
calculate MCV & MCHC
Calculate absolute no. of Eosinophil
CT-BT
calculate MCH & MCHC
ABO-Rh blood group estimation
Perform Arneth’s count
DLC & absolute count of Basophil
Perform CT-BT & change in Haemophilia &
Purpura
ABO-Rh blood group estimation
Prepare PBF & focus Monocyte

Laboratory In-charge:- Dr. ………………………………, Sign=………………. HOD:- Dr. ND Soni ,

Sign =…………

CHITS FOR SITTING ARRANGEMENT IN LAB.

S-01
S-02
S-03
S-04
S-05
S-06
S-07
S-08
S-09
S-10
S-11
S-12
S-13
S-14
S-15
S-16
S-17
S-18
S-19
S-20
S-21

S-22
S-23
S-24
S-25
S-26
S-27
S-28
S-29
S-30
S-31
S-32
S-33
S-34
S-35
S-36
S-37
S-38
S-39
S-40
S-41
S-42

S-01
S-02
S-03
S-04
S-05
S-06
S-07
S-08
S-09
S-10
S-11
S-12
S-13
S-14
S-15
S-16
S-17
S-18
S-19
S-20
S-21

S-22
S-23
S-24
S-25
S-26
S-27
S-28
S-29
S-30
S-31
S-32
S-33
S-34
S-35
S-36
S-37
S-38
S-39
S-40
S-41
S-42

S-01-LEFT
S-02-LEFT
S-03-LEFT
S-04-LEFT
S-05-LEFT
S-06-LEFT
S-07-LEFT
S-08-LEFT
S-09-LEFT
S-10-LEFT
S-11-LEFT
S-01-LEFT
S-02-LEFT
S-03-LEFT
S-04-LEFT
S-05-LEFT
S-06-LEFT
S-07-LEFT
S-08-LEFT
S-09-LEFT
S-10-LEFT
S-11-LEFT

S-01-RIGHT
S-02-RIGHT
S-03-RIGHT
S-04-RIGHT
S-05-RIGHT
S-06-RIGHT
S-07-RIGHT
S-08-RIGHT
S-09-RIGHT
S-10-RIGHT
S-11-RIGHT
S-01-RIGHT
S-02-RIGHT
S-03-RIGHT
S-04-RIGHT
S-05-RIGHT
S-06-RIGHT
S-07-RIGHT
S-08-RIGHT
S-09-RIGHT
S-10-RIGHT
S-11-RIGHT

S-12-LEFT
S-13-LEFT
S-14-LEFT
S-15-LEFT
S-16-LEFT
S-17-LEFT
S-18-LEFT
S-19-LEFT
S-20-LEFT
S-21-LEFT
S-22-LEFT
S-12-LEFT
S-13-LEFT
S-14-LEFT
S-15-LEFT
S-16-LEFT
S-17-LEFT
S-18-LEFT
S-19-LEFT
S-20-LEFT
S-21-LEFT

S-12-RIGHT
S-13-RIGHT
S-15-RIGHT
S-15-RIGHT
S-16-RIGHT
S-17-RIGHT
S-18-RIGHT
S-19-RIGHT
S-20-RIGHT
S-21-RIGHT
S-22-RIGHT
S-12-RIGHT
S-13-RIGHT
S-15-RIGHT
S-15-RIGHT
S-16-RIGHT
S-17-RIGHT
S-18-RIGHT
S-19-RIGHT
S-20-RIGHT
S-21-RIGHT