Discussion

The unknown organism #600 is Pseudomonas
Fluorescens. The unknown organsism was identified by the
Table : Data sheet, Differential Staining and Biochemical
Tests. The Gram stain test showed that the unknown was a
gram negative bacteria because it appeared pink in colour.
Looking at Table2.1 this left only four gram negative bacteria
which consequently were bacilli’s in shape. The organisms
that are left are Pseudomonas Fluorescens, Escherichia coli,
Alcaligenes faecalis, Enterobacter aerogenes. In the
experiment the bacteria grew in temperatures between 303°C 7. All four bacteria grew in that temperature range. Using
the gaspak to test for oxygen requirement we observed that
there was no growth therefore it was an obligate aerobe.
This helped us eliminate, Escherichia coli. While testing for
catalase the unknown showed a positive reaction, creating
bubbles. This left us not being able to eliminate any
organisms. We are still left with Pseudomonas Fluorescens,
Alcaligenes faecalis, Enterobacter aerogenes. In the Oxidase
test, the unknown organism turned dark blue, very much like
the positive control therefore the reaction was positive, and
did not narrow any results, again. During the citrate test the
unknown organism changed the agar from green to blue
resulting in a positive test. This allowed us to eliminate
Alcaligenes faecalis. Leaving us with only two organisms;
Pseudomonas Fluorescens and Enterobacter aerogenes. The
starch test produced a no “halo” effect around the organism
resulting in a negative result. This again did not eliminate
any microorganism. The Motility test was positive, and when
compared to the other organism Enterobacter aerogenes
and Pseudomonas Fluorescens, both produced a positive
result, not allowing us to eliminate an organism. The indole
test produced no black product (H2S) for the unknown
microorganism, again not allowing us to eliminate any
organism. The Voges-Proskauer test showed no colour
change resulting in a negative result. This did not eliminate
any organism. The Methyl Red Test showed a negative result
because it had no colour change. This test eliminated

It entails finding the nucleotide sequence. which is afterwards used to discover the organism. The 16s rRNA gene sequencing requires one to enter the internet and input the data (sequencing) and wait for the computer to find relevant anwsers. It has more information about specific species. then biochemical testing would be the other alternative in recognising the microorganism. and it does not group clinically important bacteria together like the first edition does. b) Volume 2 under phylum Proeobacteria. Class Gammaproteobacteria Order Pseudomonadales Family Pseudormonacalaceae Genus Pseudomonas c) The medical significance of the IMViC series of tests is that it helps in identifying groups of Enterobacteriacea that can potentially cause intestinal infections. The first one also provides more information about the biology of each group. If there was to be no access to the internet or finding the gene sequence was unavaliable. a) The three ways in which the second edition of Bergeys manual differs from the first edition is that the first edition is more exhaustive work. is particularly consistent identification method.Enterobacter aerogenes. with more detailed information for each genus. It also divides bacteria primarily on phenotypic characteristics not phylogenetic relatopnships The second edition is largely phylogenetic rather than phonetic. Based on the tests that the biochemical tests give out (positive and negative results) it is rather simple to identify when multiple tests are conducted under certain conditions. by using the biochemical properties and their reactions to different substrates. These are all tests that can be performed in a lab within 48 hours. 16S rRNA gene sequencing. The 16s rRNA gene sequencing would be best suited under situations or conditions where accessibility to computers and internet is available. It has more ecological information about individual taxa. Test that . This left us with the microorganism Pseudomonas Fluorescens.

Coli). Species like Escherichia coli and Salmonella are major causes. meaning it does not need any new strains of E. Escherichia coli is known as an endogenous bacteria. Eterobacteriacea are easy to grow because of their simple nutrient requirements as they can thrive in almost any environment. Methyl red test and Voges-Proskauer. Enterobacteriacea can be found in water. That is why they are of importance because they can replicate without any new addition and possible cause bodily harm .Coli to make one ill. rods. Indole test. live in the gastrin intestinal tract and they are all facultative anaerobes. . The characteristics that all enteric bacteria share are they are all gram negative. including us humans (E. d) The family Enterobacteriacea is of particular importance to the clinical microbiologists because they are major causes of intestinal tract. All Eterobacteriacea partake in the capability to ferment glucose. citrate tests. vegetation and soil and are part of the natural enteric flora of animals.can be done are.