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CHROMELEON

Chromatography Management System

Tutorial
and

User Manual
Version: 6.70
April 2005
Dionex 2005

Doc.: CM_Manual_670.doc

License Agreement

CHROMELEON Software
Individual License Agreement
This legal document is an agreement between you, the end user, and
Dionex Corporation. The CHROMELEON Chromatography Management
System is provided to you only on the condition that you agree to become
bound by the terms of this agreement, which includes the software license
and the software disclaimer warranty. If you do not agree to the terms of
this agreement, promptly return the disk package(s) and the accompanying
items (including printed materials and binders or other containers) to
Dionex for a full refund.
INDIVIDUAL CHROMELEON LICENSE
1. GRANT OF LICENSE In exchange for the payment of the LICENSE
FEE, Dionex grants the LICENSEE a non-exclusive right to use and display
the CHROMELEON software (the "SOFTWARE") provided with this LICENSE
in accordance with the terms and restrictions below. The LICENSEE is the
end-user organization and its subsidiaries (excluding sub-contractors)
billed by Dionex for this LICENSE. If the organization billed is not the enduser, the LICENSEE is the first end-user organization.
2. OWNERSHIP OF THE SOFTWARE
The LICENSEE owns the
magnetic or other physical media on which the SOFTWARE is originally or
subsequently recorded, but Dionex retains title and ownership of all
SOFTWARE recorded on such media, regardless of the form in which it is
recorded. This license is not a sale of the SOFTWARE or any copy.
3. COPY RESTRICTIONS The SOFTWARE and all accompanying written
materials are copyrighted, and may contain proprietary and confidential
information of Dionex.
The LICENSEE may make one (1) copy of the original SOFTWARE
diskettes provided with this LICENSE for backup purposes only. Any such
copy automatically becomes the property of Dionex, and the LICENSEE
must include the copyright notice and any confidentiality or proprietary
legend with any such copy. The LICENSEE may copy the SOFTWARE
from the original or backup diskettes to the computers used in accordance
with the use restrictions below.

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License Agreement

All other copying of the SOFTWARE and the accompanying materials is


expressly forbidden. The LICENSEE may be held legally responsible for
any copyright infringement that is caused or encouraged by the failure of
the LICENSEE to abide by the terms of this license.
4. USE RESTRICTIONS Under this LICENSE, the LICENSEE may use
the SOFTWARE on one (1) single-user computer, or one (1) terminal of a
multi-user computer. The LICENSEE must purchase a CHROMELEON Data
Processing License for each additional computer on which the
SOFTWARE provided under this LICENSE is used.
The SOFTWARE may be stored on the computer's local mass storage
device, on a network file server, or both, as long as the above use
restrictions are satisfied.
The LICENSEE may not modify, adapt, translate, reverse engineer,
decompile nor disassemble the SOFTWARE, nor create derivative works
based on it. The LICENSEE may not merge the SOFTWARE with another
program. The LICENSEE may not modify, adapt, translate, or create
derivative works based on the written materials without prior consent from
Dionex.
5. TRANSFER RESTRICTIONS The SOFTWARE is licensed only to the
LICENSEE, and may not be transferred to anyone else without the prior
written consent of Dionex. Any authorized transfer of the SOFTWARE shall
be bound by the terms and conditions of this LICENSE.
6. TERMINATION
This LICENSE is effective until terminated. It will
terminate automatically without notice from Dionex if the LICENSEE fails to
comply with any of its provisions. Upon termination, the LICENSEE shall
destroy the written materials and all copies of the SOFTWARE. The
LICENSEE may terminate this LICENSE by destroying all copies of the
software and accompanying written materials.
7. UPDATE POLICY Dionex may (but is not obliged to) release updated
versions of the SOFTWARE. One copy of any such versions will be
provided to the LICENSEE at no additional charge for each registered
SYSTEM that has a valid CHROMELEON Subscription Agreement
("SUBSCRIPTION") in effect at the time of the release. Any such software
update that is provided under a SUBSCRIPTION may be used to collect
data only on the one SYSTEM to which the SUBSCRIPTION applies. All
terms of this LICENSE shall apply to any updates provided to the
LICENSEE.

License Agreement

iii

8. MISCELLANEOUS This LICENSE, including the disclaimer of warranty


and limited warranty, is governed by the laws of the State of California,
excluding its choice of law rules.
DISCLAIMER OF WARRANTY AND LIMITED WARRANTY
The SOFTWARE and the accompanying materials (including instructions
for use) are provided "as is" without warranty of any kind. Further, Dionex
does not warranty, guarantee, or make any representations regarding the
use, or the results of the use, of the SOFTWARE or written materials in
terms of correctness, accuracy, reliability, currentness, or otherwise. The
entire risk as to the results and performance of the SOFTWARE is
assumed by the LICENSEE.
Dionex warrants to the original LICENSEE that the disks on which the
software is recorded are free from defects in material and workmanship
under normal use and service for a period of 90 days from the date of
delivery of this individual LICENSE as evidenced by a copy of the receipt.
The entire liability of Dionex and the exclusive remedy to the LICENSEE
shall be, at the discretion of Dionex, either (a) return of the purchase price
or (b) replacement of any defective disks that are returned to Dionex with a
copy of the receipt. If failure of the disks has resulted from accident, abuse,
or misapplication, Dionex shall have no responsibility to replace the disks
or refund the purchase price. Any replacement disk shall be warranted for
the remainder of the original warranty period or thirty (30) days, whichever
is longer.
THE ABOVE ARE THE ONLY WARRANTIES OF ANY KIND AND
DIONEX HEREBY DISCLAIMS ALL OTHER WARRANTIES, EITHER
EXPRESSED OR IMPLIED, INCLUDING (BUT NOT LIMITED TO) THE
IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR PURPOSE, THAT ARE MADE BY DIONEX ON THIS
PRODUCT. No oral or written information or advice given by Dionex, its
dealers, distributors, agents, or employees shall create a warranty or in any
way increase the scope of this warranty, and the LICENSEE may not rely
on such information or advice.
NEITHER DIONEX NOR ANYONE ELSE WHO HAS BEEN INVOLVED IN
THE CREATION, PRODUCTION, OR DELIVERY OF THIS SOFTWARE
SHALL BE LIABLE FOR ANY DIRECT, INDIRECT, CONSEQUENTIAL,
OR INCIDENTAL DAMAGES (INCLUDING DAMAGES FOR LOSS OF
BUSINESS PROFITS, BUSINESS INTERRUPTION, LOSS OF BUSINESS
INFORMATION, AND THE LIKE) ARISING OUT OF THE USE OF OR
INABILITY TO USE THIS SOFTWARE, EVEN IF DIONEX HAS BEEN

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License Agreement

ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. IN THE EVENT


THAT ANY LIABILITY IS IMPOSED ON DIONEX, THE LIABILITY OF
DIONEX TO THE LICENSEE OR ANY THIRD PARTY SHALL NOT
EXCEED THE PURCHASE PRICE OF THE SOFTWARE.
Some states do not allow exclusion or limitation of the duration of the
implied warranties or exclusion or limitation of liability for incidental or
consequential damages, so that the above limitations may not apply to you.
This warranty gives you specific legal rights, and you may have other
rights, which vary from state to state.

Abbreviations

Trademarks and Product Names


Below please find a list containing various trademarks and product names.
All trademarks () and registered trademarks () belong to Dionex
Corporation unless indicated otherwise. All other trademarks or registered
trademarks are the property of their respective owners.

A
AAA-Direct Amino Acid Analysis System
AAA-Certified
AAES
Acclaim columns
Acrodisk (Gelman Sciences, Inc.)
Acurate Flow Splitter
Advanced Computer Interface (ACI)
Advanced Gradient Pump (AGP)
AD20 UV/Vis Absorbance Detector
AES Atlas Electrolytic Suppressor
Alliance (Waters Corp.)
AminoPac Columns
AminoTrap Columns
AMMS-ICE, AMMS-ICE II Anion MicroMembrane Suppressor
AnchorChips (Bruker BioSciences)
Anion Atlas Electrolytic Suppressor
Anion Self-Regenerating Suppressor
Anotop (Whatman)
AppliCard
AQA Mass Spectrometer (Thermo Electron Corp.)

Abbreviations

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ARC Automated Run Completion


Aroclor (Monsanto Corp.)
AS Autosampler
AS40, AS50 Automated Sampler
AS3500 Autosampler
ASE 100, 200, or 300 Accelerated Solvent Extractor or Solvent Controller
ASI-100, ASI-100T, ASI-100P, or ASI-100PT Automated Sample
Injector
ASRN Anion Self-Regenerating Neutralizer
ASRS, ASRS
Suppressor

II,

or

ASRS

ULTRA

Anion

Atlas Suppressor
Auto OnGuard (but OnGuard)
AutoASE software
autoflex (Bruker BioSciences)
Autoion
AutoNeutralization system or technology
AutoQ
AutoRegen System
AutoSelect
AutoSuppression device
AXIMA-QIT (Shimadzu Biotech)
B
BAKER INSTRA-ANALYZED Acids (J. T. Baker)
Biodialyser (AmiKa, Inc.)
BioLC System
BioPlus Columns
BioSelect (The Separations Group)
BorateTrap Column

Self-Regenerating

Abbreviations

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C
CA Carbamate Analyzer
CAES Cation Atlas Electrolytic Suppressor
CarboPac MA1, PA1, PA10, PA-100 columns
Cation Atlas Electrolytic Suppressor
Cation MicroMembrane Suppressor
Cation Self-Regenerating Suppressor
CD20 Conductivity Detector
CD Builder (AppletWare, Inc.)
Chemraz (Greene, Tweed & Co.)
ChromatoCritters
CH-2 Column Heater
CHX650 Column Temperature Controller
CHROMELEON Chromatography Management Software
CMD Carbohydrate Membrane Desalter
CMMS Cation MicroMembrane Suppressor
Continuously Regenerating Anion Trap Column (CR-ATC)
Continuously Regenerating Cation Trap Column (CR-CTC)
CSRN Cation Self-Regenerating Neutralizer
CSRS, CSRS II, CSRS ULTRA Cation Self-Regenerating Suppressor
CMMS II Cation MicroMembrane Suppressor
Cryptand columns

D
DataDetective (AppletWare Inc.)
dBASE (Borland International, Inc.)
Dequest (Monsanto Corporation)
Dionex

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Abbreviations

DNAPac column (for NucleoPac)


DNAPhor SB1.5 kB Sieving Buffer Kit (for NucleoPhor)
Dowex (Dow Chemical Company)
DX-80 Ion Analyzer
DX-120 Ion Chromatograph
DX-320 Ion Chromatograph
DX-500 or DX-600 Ion Chromatography System or HPLC System
DX-800 Process Analyzer
DX-LAN Instrument Interface
E
ED40, ED50 Electrochemical Detector
EG40, EG50 Eluent Generator
Electrochrom
EluGen
EO1 Eluent/Solvent Organizer
F
FAMOS Fully Automated Micro Autosampler
FastLoc (Thermo Electron Corp.)
Finnigan AQA, Finnigan MSQ
Flarefit
FluoBoost Micro Fluorescence Flow Cell
Foxy (Isco, Inc.)
FPLC (Pharmacia LKB)
Freon (E.I. du Pont de Nemours & Co.)
Fusica columns

Abbreviations
G
GM-2, GM-3, or GM-4 Eluent Gradient Mixers
GP40, GP50 Gradient Pump
GS50 Gradient Pump
H
HPICE (mostly replaced by IonPac ICE)
Hydromatrix (Varian Associates, Inc.)
I
ICE (ion-exclusion columns, e.g., ICE-AS6)
ICS-90, ICS-1000, ICS-1500, ICS-2000, ICS-2500
ICS-3000 Conductivity Detector
ICS-3000 Detector/Chromatography
ICS-3000 Dual Pump
ICS-3000 Electrochemical Detector
ICS-3000 Eluent Generator
ICS-3000 Single Pump
InkJet (Hewlett-Packard)
IonPac columns
IonPhor Electrolyte Buffers
IonSep reagents
IP20 Isocratic Pump
Irganox (Ciba)
J
Just Add Water

ix

Abbreviations

K
Kalrez (E.I. du Pont de Nemours & Co.)
KEL-F (3M Corporation)

L
LANtastic (Artisoft, Inc.)
LaserJet (Hewlett-Packard Corporation)
LC5 Injection Module
LC10 Chromatography Organizer
LC20 Chromatography Enclosure
LC25, LC30 Chromatography Oven

M
Mascot (Matrix Science Ltd.)
MassLynx (Micromass)
-Guard columns
-Dumper
-Fluidics
MetPac Reagents
MFC-1 (Metal-Free Column)
MICRO (International Products Corp.)
Micro Precolumn
MicroBead resin
MicroInjection valve
MicroMembrane Suppressor
Millennium (Waters Corp.)
Milli-Q (Millipore)
MMS MicroMembrane Suppressor

Abbreviations
Monolithic capillary column
Mono Q (Pharmacia LKB)
MonoStandard
M Path (Thermo Electron Corp.)
MPIC (Mobile Phase IC)
MRA Active Flow Splitter (Rheodyne)
MS-DOS (Microsoft Corporation)
MSQ Mass Spectrometer (Thermo Electron Corp.)
N
Nano precolumn
N-EVAP (Organomation Associates, Inc.)
NovaPak (Waters Corp.)
NucleoPac (now DNAPac)
NucleoPhor (now DNAPhor)
O
OligoStandards
OmniFLEX (Bruker BioSciences)
OmniPac Columns
OnGuard, OnGuard II Sample Prep Station (but Auto OnGuard)
Optima (Fisher Scientific)
ORBO (Supelco, Inc.)
P
P680 HPLC Pump
PaintJet (Hewlett-Packard)
PC10 Postcolumn Pneumatic Controller

xi

xii

Abbreviations

PC10 Reagent Delivery Module


PD40 Diode Array Detector
PDA-100 Photodiode Array Detector
PeakNet Chromatography Workstation
Pentium (Intel)
PepMap
Pico-Buffer
PicoView (New Objective, Inc.)
PolyVial
PowerPoint (Microsoft)
Probot Microfraction Collector
Process 450 (Datensystem fr 8200er-Serie)
ProPac Columns
ProteinChips (Ciphergen)
Purification Suite
PWA Purification Workflow Automation
Q
QSTAR (Applied Biosystems)
R
Reacti-Therm (Pierce Chemical Company)
Reagent-Free

Abbreviations
S
SC-CSRS
SELDI ProteinChips (Ciphergen)
Self-Contained Cation Self-Regenerating Suppressor
SelectaPore Columns (The Separations Group)
Self-Regenerating Suppressor
SEQUEST (University of Washington, Seattle, USA)
Series 600 SFC and SFC/GC Systems
SFE-723 Supercritical Fluid Extractor
SFM Sample and Fraction Manager
SmartFlow
SP10 AutoNeutralization System
SpeedVac (Savant Corp.)
SRC SRS Controller)
SRN Self-Regenerating Neutralizer
SRS Self-Regenerating Suppressor
Summit HPLC System
SUPELCOSIL, Supelguard (Supelco, Inc.)
Superba columns
Superose (Pharmacia)
Supor (Pall Corporation)
SupraPur (EM Industries, Inc.)
Switchos Microcolumn Switching Device
T
TAC-1 (Trace Anion Concentrator)
TCC-1 (Trace Cation Concentrator)
TCC-100 (Thermostatted Column Compartment)

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xiv

Abbreviations

Teflon, Tefzel (E.I. du Pont de Nemours & Co.)


ThermoFlare
Thermos Column Oven
Triton X-100 (Rohm & Haas)
TurboVap (Zymark Corporation)
Tween 20 (Atlas Chemical Co.)
U
UCI-100 or UCI-50 Universal Chromatography Interface
UI20 Universal Interface
ultraflex (Bruker BioSciences)
UltiChrom software
UltiMate
Ultrex (J.T. Baker)
UV-Booster
UVD 170S/170U UV/Vis Detector
UVD 340S/340U Photodiode Array Detector
U-Z View Capillary Flow Cell
V
Vespel (E.I. du Pont de Nemours & Co.)
VHP (The Separations Group, Inc.)
Vortex-Genie (Scientific Industries, Inc)
Voyager (Applied Biosystems)
Vydac columns (The Separations Group, Inc.)

Abbreviations
W
Windows 2000/XP (Microsoft Corporation)
Windows NT (Microsoft Corporation)
Wonderware InTouch (Wonderware Corporation)

Z
Zitex (Norton Chemplast)
Zorbax (E.I. du Pont de Nemours & Co.)

xv

xvi

Abbreviations

Abbreviations

xvii

Abbreviations
Below please find a list of the most frequently used abbreviations in
Chromeleon, the online Help, and the manual:
APS

Automated Purification System

ARC

Automated Run Completion

AIA

Analytical Instrument Association

BCD

Binary-Coded Decimal Code

BOOTP Bootstrap Protocol (Internet protocol)


CAN

Controller Area Network

CD

Compact Disk

CDS

Chromatography Data System

CE

Capillary Electrophoresis

CFR

Code of Federal Regulations


(often synonymous with 21 CFR Part 11)

CM

Chromeleon Chromatography Management System

CMB

Backup container/file (file extension)

CMS

Chromatography Management System

CS

Cluster Server

DHCP

Dynamic Host Communication Protocol

DNS

Domain Name Server

DX

Dionex

GC

Gas Chromatography

GLP

Good Laboratory Practice

GMP

Good Manufacturing Practice

GPIB

General Purpose Interface Bus

HPLC

High Performance Liquid Chromatography

IC

Ion Chromatography

Abbreviations

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IPC

Chromeleon Instruments Server or Instruments PC

IQ

Installation Qualification

ISTD

Internal Standard

LAN

Local Area Network

LIB

UV Spectra Library (file extension)

LIC

Chromeleon License Server

LIMS

Laboratory Information Management System

LOD

Limit of Detection

MAC

Media Access Control address


(unique address of each network interface card)

MRA

Mass Rate Attenuation (Flow Splitter)

MS

Mass Spectrometer

MSV

Motorized switching valve

NFP

Network Failure Protection

ODBC Microsoft Open Database Connectivity


OQ

Operational Qualification

PAN

Control Panel for chromatographic system control


(file extension)

PDA

Photodiode Array Detector

PGM

Control program for chromatographic system control


file extension)

PN

PeakNet Chromatography Management System

PPA

Peak Purity Analysis

PPI

Peak Purity Index

PQ

Performance Qualification

PWA

Purification Workflow Automation

QNT

Quantification method for signal interpretation and evaluation (file


extension)