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Exercise 2: Skeletal Muscle Physiology: Activity 2: The Effect of Stimulus Voltage on Skeletal Muscle Contraction Lab

Report
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
1. Skeletal muscle fibers are innervated (stimulated) by
You correctly answered: c. motor neurons.
2. A single action potential propagating down a motor axon results in
You correctly answered: d. a single action potential and a single contractile event in the muscle fibers it innervates.
3. In resting skeletal muscle, calcium is stored in
You correctly answered: c. the sarcoplasmic reticulum.
4. During the latent period for an isometric contraction
You correctly answered: c. the cellular events involved in excitation-contraction coupling occur.

03/20/15

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19 1.70 1. and why does this observation make sense? You correctly answered: a.5 3.66 0.59 1.00 0.0 4.00 0.0 2.00 0.78 1.00 0.0 Length 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 75 Active Force 0.15 0.82 1.00 0.43 0.65 1.5 9. there was no activation of skeletal muscle fibers by this stimulus.00 0.59 1.82 1.5 2.51 1.65 1.02 0.0 1. 0.66 0.5 4.00 0.81 1.00 0.00 0. Enter the threshold voltage for this experiment in the field below and then click Submit to record your answer in the lab report You answered: 8 volts 12.82 1.43 0.5 5. Enter the maximal voltage for this experiment in the field below and then click Submit to record your answer in the lab report.00 0.82 03/20/15 Passive Force 0.5 6. Stop & Think Questions: What do you see in the active force display when the stimulus voltage is set to 0.0 0.0 9.82 1.00 0.81 1. The active force will first increase and then plateau at some maximal value as the stimulus voltage increases.5 7.0 7.0 3.82 .82 1.8 1.00 0.00 0.00 0.Experiment Results Predict Question: Predict Question: As the stimulus voltage is increased from 1.00 0. what will happen to the amount of active force generated with each stimulus? Your answer : c.70 1.32 1.87 1.00 0.00 0.32 1.0 5.00 0.82 1.0 8.19 1.00 page 2 Total Force 0. You answered: 10 volts Experiment Data: Voltage 0.42 1.00 0.74 1.04 1.5 8.00 0.00 g.04 1.5 10.87 1. threshold voltage 6. What is the lowest stimulus voltage that induces active force in the skeletal muscle? You correctly answered: b.42 1.51 1.0 6.00 0.0.78 1.00 0.15 0.74 1.0 volt up to 10 volts.02 0.00 0.2 0.00 0.00 0.

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maximal voltage 5. You correctly answered: c. 3. they were all successfully recruited). Why was a maximal voltage observed in this experiment? You correctly answered: b. all the muscle fibers contained in this muscle are depolarized and they all develop active force (that is. an increase in the number of active muscle fibers to increase the force developed in a muscle. activation of cross bridge cycling via increased intracellular calcium levels. Motor unit recruitment refers to You correctly answered: a. the ________ is the amount of stimulus required to successfully recruit all the muscle fibers into developing active force. You correctly answered: d.Post-lab Quiz Results You scored 100% by answering 5 out of 5 questions correctly. 1. The ________ is the minimal stimulus needed to cause a depolarization of the muscle plasma membrane (sarcolemma). 2. Active tension (or force) in a skeletal muscle fiber results from You correctly answered: a. By definition. 03/20/15 page 11 . threshold voltage 4. At the maximal voltage.

Therefore. Describe the effect of increasing stimulus voltage on isolated skeletal muscle. the electric current must surpass the threshold voltage. The Maximal tension has a maximum amount of voltage that it will achieve toincrease the force. each of thefibers were involved in the active force generated from the maximal voltage. 03/20/15 page 12 . Specifically. what happened to the muscle force generated with stronger electrical stimulations and why did this change occur? How well did the results compare with your prediction? Your answer: As the stimulus voltage is increased past the muscle’s threshold voltage. This occurs becauseas the voltage increases. 3. Whole-Muscle Force is achieved in Vivo by recruitment of additional motorunits that increase the total muscle force produced. How is this change in whole-muscle force achieved in vivo? Your answer: The increase of electrical current causes a progressive increase in the amount of muscle force based on the amount of theelectrical current. What happened in the isolated skeletal muscle when the maximal voltage was applied? Your answer: All of the muscle fibers in the isolated skeletal muscle became depolarized and developed an active force. At the beginning stage. more fibers are activated and causes total force produced bythe muscles to increase. My prediction was partially correct. Each time the voltage was increased the force generated from the muscle also increased. Increasingly the force gets stronger with the higher stimulusvoltage until it plateaus at Maximal tension. and is delivered to the whole muscle. 2. Once the stimulus voltagegoes beyond the threshold it creates whole muscle force.Review Sheet Results 1. the amount of force in the entire muscle increases. but only to a maximum point.