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Measuring the Health

of the Environment

6800 Campobello Rd
Mississauga, Ont, Canada
905.487.7359 ph
905.794.2338 fax

CUSTOM SOLUTIONS At EBPI we strive to meet the demands of our clients and their changing requirements. A modified SOS-ChromoTest bioassay using a !The outer membrane of the cell was modified to increase chromogenic pad was developed to test for genotoxicity in permeability to many materials. any induction of degree of DNA damage the cell experiences by the SOS response will result in production of â- observing the colour obtained. exponential growth phase in tubes containing 0. the SOS Inducing Potency (SOSIP) is simply calculated SOS genes are fused to the LacZ by determining the slope of the linear portion of the plot.Determination photograph above of the genotoxicity of water and waste water using the umuC-test) where the Once the test is conducted. which can be assayed using a simple ENGINEERING OF THE STRAIN !Detects induction of the SOS genes which are involved in A more quantitative value may be obtained by colour change. The solid particulates are then The principle of the UMU-CHROMOTEST washed off and genotoxicity is noted by the presence of a distinctive blue colour on the pad as shown below. galactosidase. or by using EBPI’s excel software included with the kit . genes. All living cells have developed a sensitive ChromoTest before the cell's repair system has had the system for the detection of lesions in their genetic chance to handle the emergency. which can be performed in a non-specialized SOS repair system promoter is induced to start the transcription of the SOS laboratory. an SOS procedure. For further information: material so that complex enzymatic systems such as the can be activated to repair the EBPI has developed the SOS-ChromoTest into a simple damage. which can be purchased separately contains all reagents and enzymes required to preform an in vitro metabolic activation to mimic mammalian liver metabolism. The test is based on the de novo synthesis of B-galactosidase enzyme in a !The SOS promoter does not activate the SOS system.1 gram of sediment. The S9 Activation Kit. instead it induces the synthesis of the readily detectable âgalactosidase enzyme. Once a lesion has been detected. sediments directly without extraction. coli. genetically-engineered strain of E. manually. In just a few hours. THE SEDIMENT SOS-CHROMOPAD TEST DNA repair spectrophotometry using a micro-plate reader. which when it comes in contact with An antibiotic-containing culture is placed into an a chromogenic substrate catalyses the formation of colour. be repaired but rather the â-galactosidase enzyme gene the SOS-ChromoTest Kit is available with a cofactorsupplemented post-mitochondrial fraction (S9) prepared from the liver of rats treated with enzyme-inducing agent Aroclor 1254. Following the incubation. the kit provides a clear. This can be calculated reporter gene. Serial dilutions are then preformed and the bacteria cultures are incubated for 4 hours. even limited repairable damage the cell's own mechanisms for the detection of to the genetic material will be detected by the SOS- genotoxicity. A qualitative evaluation of the degree A quantitative value may be obtained kit is based on the International of DNA damage the cell experiences is by spectrophotometry using a micro- Organization for Standardization protocol shown on the photograph above plate reader is shown on the ISO 13829 (Water Quality. completely objective measurement of the genotoxicity of By fusing the LacZ gene (responsible for the production a sample by a simple visual qualitative evaluation of the of â-galactosidase) to the SOS gene. a drop of each mixture is placed ALSO AVAILABLE THE UMU-CHROMOTEST KIT onto a chromgenic pad and an additional incubation for 20 hours is conducted. please contact us at www.THE PRINCIPLES OF THE SOS-CHROMOTEST The SOS-ChromoTest kit is designed for Rapid This is the basis for the dependability and sensitivity of Detection of Genotoxicity or DNA Damage and utilizes the SOS-ChromoTest. !SOS genes are fused in the LacZ reporter gene S9 ENZYME ACTIVATION AND THE SOSCHROMOTEST !The strain's own repair system was altered by a series of As many classes of compounds are inactive until mutations so that even limited damage to the DNA will not processed by an appropriate metabolic activation system.