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M330

METTLER TOLEDO

Tartaric acid in wine


General method for the determination of acidity of wines

Sample

50 mL wine from grapes

Preparation and Procedures


The samples should be degassed in an ultrasonic bath for five
minutes to get rid of CO2.

Substance

Tartaric acid, (CHOH)2(COOH)2 ;


M=150.09 g/mol; z= 2

Before the analysis a three point calibration of the electrode was


performed using METTLER TOLEDO buffers of pH 4.01, 7.00
and 9.21.

Chemicals

Titrant

Sodium hydroxide, NaOH


c(NaOH) = 1.0 mol/L;

Standard

Potassium hydrogen phthalate, 80 mg

Instruments

DL22 F&B, DL28


OKI B4250 Printer
(parallel cable ME-51 108 780)

Accessories

Titration beakers ME-101974

Remarks
Indication

DG115-SC

Chemistry

H2C4H4O6 + 2NaOH <=> Na2C4H4O6 +


2H2O

Calculation

Tartaric acid is chosen to express the


acid content in g/L in wine because it is
the predominantly present acid.

The principle acids found in grapes are tartaric and malic acids.
Their proportions vary depending on the variety of grapes,
however in the wine tartaric dominates.
Unripe grapes are sour due to the high concentration of tartaric
acid. When the grapes ripen, much of this acid is converted to
sugar. Then during the fermentation process the yeasts then
convert these sugars into ethanol and carbon dioxide.
In the determination of total acidity in wine all the acid is
assumed to be tartaric, so that the result is expressed as grams
tartaric acid per liter of wine.
Total acidity of the wine affects taste, color, as well as resistance
to microbial spoilage.

Results

Waste
disposal

No special treatment is necessary.

Author

M. Biber

Results
------------------------------------------------------------------Method ID
930
Title
Tartaric acid
08-04-2005
11:17
Author
M. Biber
------------------------------------------------------------------All results
Sample
Sample ID
Result
Calculat.
Value
Unit
------------------------------------------------------------------1
dry wine
1
Cont.
5.327
[g/L]
2

dry wine

Cont.

5.395

[g/L]

dry wine

Cont.

5.393

[g/L]

dry wine

Cont.

5.379

[g/L]

Statistics report
Result
n
x
s
Unit
srel [%]
------------------------------------------------------------------1
4
5.385
0.011
[g/L]
0.209
Results
Active sample
Deleted samples
------------------------------------------------------------------1
2
3
4

Table of measured values

Titration curve

Method
Method ID
22-03-2005

930
13:26

Sample para.
Sample ID . . . . . .
Entry type . . . . .
Volume [mL] . . . . .
Density [g/mL] . . .
Mol.m. [g/mol] . . .
Equival. number z . .
Temp.sensor . . . . .
Temp. [C] . . . . .
Stir
Speed [%] . . . . . .
Mixing time [s] . . .
EP titration
Titrant . . . . . . .
Conc.[mol/L] . . .
Sensor . . . . . . .
Unit . . . . . . .
Blank . . . . . . . .
Value [mmol] . . .
Predis.[mL] . . . . .
Back value [mmol] . .
Control parameters
Set of parameters
Addition . . . . .
dE(set) [mV] . . .
dVmin [mL] . . . .
dVmax [mL] . . . .
Measure mode . . .
dE[mV] . . . . . .
dt[s] . . . . . .
t(min) [s] . . . .
t(max) [s] . . . .
Recognition
Tendency . . . . .
Termination param.
Vmax [mL] . . . .
Evaluation
Numb. EPs . . . .
End point 1 . . .
Potential 1 [pH] .
Calculation 1
Calculation . . . . .
Type . . . . . . . .
Unit . . . . . . . .
Which EQP . . . . . .
Decimal places . . .
Result limits . . . .
Statistics . . . . .
Calculation 2
Calculation . . . . .
Calculation 3
Calculation . . . . .
Report
Output . . . . . . .
Results . . . . . . .
Meas. val. table . .
E - V curve . . . . .
dE/dV curve . . . . .

Title
Author

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Tartaric acid
M. Biber

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dry wine
Volume
50.0
1.0
150.09
2
Manual
25.0

. . . . . . . . . 35
. . . . . . . . . 10
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NaOH 1.0
1.0
DG115
pH
Entry
0.00
2.0
0.00

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User
Dynamic
8
0.020
0.2
EQU controlled
0.5
1
3
30

Other Control parameters


Set of parameters
Addition . . . . .
dE(set) [mV] . . .
dVmin [mL] . . . .
dVmax [mL] . . . .
Measure mode . . .
dE[mV] . . . . . .
dt[s] . . . . . .
t(min) [s] . . . .
t(max) [s] . . . .

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Fast
Dynamic
16
0.05
0.4
EQU controlled
1.0
1
2
20

Set of parameters
Addition . . . . .
dE(set) [mV] . . .
dVmin [mL] . . . .
dVmax [mL] . . . .
Measure mode . . .
dE[mV] . . . . . .
dt[s] . . . . . .
t(min) [s] . . . .
t(max) [s] . . . .

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Cautious
Dynamic
8
0.02
0.2
EQU controlled
0.5
2
7
40

. . . . . . . . . Positive
. . . . . . . . . 10.0
. . . . . . . . . 1
. . . . . . . . . Absolute
. . . . . . . . . 7.00
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Cont.
Cont.
g/L
1
3
No
Yes

. . . . . . . . . None
. . . . . . . . . None
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Printer
GLP
Yes
Yes
Yes