Name: ________________________ Class: ___________________ Date: __________

ID: A

Sp 2015 J201 Quiz II

Multiple Choice
Identify the choice that best completes the statement or answers the question.

1. The purpose of staining cells on a microscope

4. Which of the following compounds can destroy

slide is to

all microbial life?

a.

enlarge the cells.

a.

Steriliants

b.

secure them to the slide.

b.

Disinfectants

c.

add contrast in order to see them better.

c.

Antiseptics

d.

see motility.

d.

Sanitizers

5. You find colorless areas in cells in a

2. Which of the following agents is added to

water supplies to prevent the spread of

potential pathogens?

gram-stained smear. What should you apply

next?

a.

Bleach

a gram Stain

b.

Chlorine

a.
b.

c.

Alcohol

c.
d.

a simple stain

d.

Iodine

an endospore stain
an acid-fast stain

6. A student is looking at a bacterial specimen

3. You are performing a Gram stain on

using the oil immersion lens, but has forgotten

to put immersion oil on the slide. The specimen
will appear

gram-positive bacteria and you stop after the

addition of the first dye. What is the appearance
of the bacteria at this point?

a.
a.
b.
c.

Colorless
Red
Purple

smaller than it would if immersion oil was

used.

b.

somewhat fuzzy and have poor resolution.

d.

Brown

c.

larger than it would if immersion oil was

used.

d.

the same as it would if the immersion oil

was used.

Name: ________________________

ID: A

7. Assume you stain Bacillus by applying

11. You are performing a Gram stain on

malachite green with heat and then

counterstaining with safranin. Through the
microscope, the green structures are

gram-positive bacteria and you stop after the

addition of the counterstain. What is the
appearance of the bacteria at this point?

a.
b.
c.
d.

a.
b.
c.
d.

Endospore
Cell Walls
Cannot determined
Capsules

Red
Red
Colorless
Purple

12. Which step in the Gram stain is the critical step

8. What do the Gram stain, acid-fast stain, and

in differentiating gram-positive cells from

gram-negative cells?

endospore stain have in common?

a.

They have nothing in common

b.

use heat to force the dye into cell structures

a.

safranin

c.

used on a wet mount of the specimen

b.

iodine

d.

outcome based on cell wall differences

c.

crystal violet

d.

alcohol-acetone

9. A sputum sample is ordered for microbial

analysis in order to rule out the diagnosis of

tuberculosis. Suspecting Mycobacterium
tuberculosis may be the pathogen, you know
that the laboratory technicians will perform
which stain on the sample?
a.

endospore stain

b.

acid-fast stain

c.
d.

Gram Stain
Capsule Stain

13. Which of the following is NOT true regarding

the acid-fast stain?

a. If cells are acid-fast, they are
gram-negative.

10. Which of the following places the steps of the

Gram stain in the correct order?

1-Alcohol-acetone
2-Crystal violet
3-Safranin
4-Iodine
a.

4-3-2-1

b.

1-2-3-4

c.

2-1-4-3

d.

2-4-1-3

b.

Acid-fast cells appear red in a completed

acid-fast stain.

c.

Acid-fast cells retain the primary dye after

treatment with acid-alcohol.

d.

It is used to identify members of the genus

Mycobacterium.

14. Which type of stain is most useful in helping

clinicians to decide which antibiotic to

prescribe for a bacterial infection?
a.
b.
c.
d.

Negative Stain
Endospore Stain
Acid Fast Stain
Gram Stain

Name: ________________________

ID: A

15.

18. Bacterial smears are fixed before staining to

a.

make their walls permeable

b.

accept stain.

c.

make the cells visible.

d.

affix the cells to the slide.

19. Gram stain results from the patient specimen

indicate a bacterial infection. From your

microbiology course, you remember that this
staining procedure involves
a.

application of the dye, carbol fuchsin,

followed by an acid alcohol rinse.

b.

forcing a dye into resistant bodies with heat

to distinguish between spores and cells.

c.

application of India ink to detect the

presence of bacterial capsules

d.

timed, sequential applications of crystal

violet dye, iodine, an alcohol rinse, and a
contrasting counterstain to the sample.

Line C Points to the?

a.

condenser.

b.

illuminator.

c.

objective lens.

d.

ocular lens.

16. The Gram staining procedure is best described

as a/an __ staining technique.

a.

differential

b.

Schaefer-Fulton

c.

acid fast or Ziehl-Neelson

d.

capsule

20. Cells that are not acid fast appear ________

after the acid-alcohol step in the acid-fast

staining process.

17. You are performing a Gram stain on

gram-negative bacteria and you stop after the

decolorizer step. What is the appearance of the
bacteria at this point?
a.
b.
c.
d.

a.

Blue

b.

Clear

c.
d.

Purple
Red

21. You are performing a Gram stain on

gram-negative bacteria and you stop after the

addition of the mordant. What is the
appearance of the bacteria at this point?

Red
Brown
Colorless
Purple

a.
b.
c.
d.

Purple
Red
Brown
Colorless

Name: ________________________

ID: A

22. The purpose of a mordant in the Gram stain is

to
a.

remove the simple stain.

b.

prevent the crystal violet from leaving the

cells.

c.

make the bacterial cells larger.

d.

make the flagella visible.

23. A microbiologist makes a fixed smear of

bacterial cells and stains them with Loeffler's

methylene blue. All the cells appear blue under
the oil lens. This is an example of
a.

capsule staining.

b.

negative staining.

c.

using the acid-fast stain.

d.

simple staining

24. What is the material contained within the cell

walls of acid-fast bacteria that prevents most

stains from penetrating the cells?
a.

Mycolic acid

b.

Lipopolysaccharide

c.

Peptidoglycan

d.

Glycolipid

25. Which of the following is NOT classified as a

disinfectant?
a.

Sanitizers

b.

Antiseptics

c.

Steriliants

ID: A

Sp 2015 J201 Quiz II

Answer Section
MULTIPLE CHOICE
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25.

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C
B
C
A
B
B
A
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B
A
B
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C
B

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