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Exercise 4: Endocrine System Physiology: Activity 1: Metabolism and Thyroid Hormone Lab Report

Pre-lab Quiz Results
You scored 100% by answering 6 out of 6 questions correctly.
1. Which of the following statements about metabolism is false?
You correctly answered: d. All of the energy from metabolism is ultimately stored in the chemical bonds of ATP.
2. Thyroxine is
You correctly answered: c. the most important hormone for maintaining the metabolic rate and body temperature.
3. Thyroid-stimulating hormone (TSH) is
You correctly answered: b. produced in the pituitary gland.
4. An injection of TSH to an otherwise normal animal will cause which of the following?
You correctly answered: d. goiter development
5. Thyrotropin-releasing hormone (TRH) is
You correctly answered: a. secreted by the hypothalamus.
6. Which of the following statements is true?
You correctly answered: b. The hypothalamus primarily secretes tropic hormones that stimulate the secretion of other
hormones.

04/22/12

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Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr Enter the metabolic rate in the field below and then click Submit to display your results in the grid. The BMR of both remaining rats will be lower than the normal rat's BMR. Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr Enter the metabolic rate in the field below and then click Submit to display your results in the grid. The normal rat will become hyperthyroidic but will not develop a goiter. Predict Question 3: What do you think will happen after you inject TSH into the three rats? Your answer : a. Calculate the oxygen consumption per hour for this rat using the following equation. You answered: 432 ml O2/hr 1i. The normal rat will become hypothyroidic and develop a goiter. Your answer : c. ml O2 consumed/1 minute x 60 minutes/hour = ml O2/hour Enter the oxygen consumption per hour in the field below and then click Submit to display your results in the grid. Predict Question 2: What do you think will happen after you inject thyroxine into the three rats? Your answer : d. 04/22/12 page 2 . ml O2 consumed/1 minute x 60 minutes/hour = ml O2/hour Enter the oxygen consumption per hour in the field below and then click Submit to display your results in the grid. You answered: 1721. Calculate the oxygen consumption per hour for this rat using the following equation.Experiment Results Predict Question: Predict Question 1: Make a prediction about the basal metabolic rate (BMR) of the remaining rats compared with the BMR of the normal rat you just measured. Stop & Think Questions: 1h. Now that you have calculated the oxygen consumption per hour for this rat. Predict Question 4: Propylthiouracil (PTU) is a drug that inhibits the production of thyroxine by blocking the attachment of iodine to tyrosine residues in the follicle cells of the thyroid gland (iodinated tyrosines are linked together to form thyroxine). What do you think will happen after you inject PTU into the three rats? Your answer : d. The normal rat will become hyperthyroidic and develop a goiter.12 ml O2/kg/hr 2h. you can calculate the metabolic rate per kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms to use this equation). Now that you have calculated the oxygen consumption per hour for this rat. you can calculate the metabolic rate per kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms to use this equation). You answered: 378 ml O2/hr 2i.

The hypophysectomized (Hypox) rat is You correctly answered: a. BMR above 1800) after the thyroxine injection. you can calculate the metabolic rate per kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms to use this equation). The normal rat is You correctly answered: b. categorize the rats as hypothyroid (low thyroid levels. euthyroid ("good. categorize the rats as hypothyroid (low thyroid levels. or hyperthryoid (high thyroid levels. BMR = 1650-1750). Now that you have calculated the oxygen consumption per hour for this rat." or normal. or hyperthryoid (high thyroid levels. euthyroid Judging from their basal metabolic rates (an indicator of thyroid function). or hyperthryoid (high thyroid levels. categorize the rats as hypothyroid (low thyroid levels. BMR above 1800)." or normal. categorize the rats as hypothyroid (low thyroid levels. euthyroid ("good. euthyroid ("good. BMR below 1600). BMR = 1650-1750). ml O2 consumed/1 minute x 60 minutes/hour = ml O2/hour Enter the oxygen consumption per hour in the field below and then click Submit to display your results in the grid. 04/22/12 page 3 . hyperthyroid Judging from their basal metabolic rates (an indicator of thyroid function). The normal rat is You correctly answered: c. You answered: 384 ml O2/hr 3i. hypothyroid Judging from their basal metabolic rates (an indicator of thyroid function). BMR above 1800) after the thyroxine injection. The thyroidectomized (Tx) rat is You correctly answered: a. BMR = 1650-1750). BMR = 1650-1750). thyroid levels. euthyroid ("good. euthyroid ("good. BMR above 1800). BMR below 1600). BMR below 1600). Metabolic rate = (ml O2/hr)/(weight in kg) = ml O2/kg/hr Enter the metabolic rate in the field below and then click Submit to display your results in the grid. BMR below 1600).35 ml O2/kg/hr Judging from their basal metabolic rates (an indicator of thyroid function). hypothyroid Judging from their basal metabolic rates (an indicator of thyroid function). BMR above 1800). thyroid levels.You answered: 1542. thyroid levels.86 ml O2/kg/hr 3h. categorize the rats as hypothyroid (low thyroid levels. You answered: 1567. Calculate the oxygen consumption per hour for this rat using the following equation. BMR = 1650-1750)." or normal." or normal. thyroid levels. BMR below 1600). or hyperthryoid (high thyroid levels. or hyperthryoid (high thyroid levels. thyroid levels." or normal.

the hypophysectomized rat Judging from their basal metabolic rates (an indicator of thyroid function). The normal rat is You correctly answered: a. Judging from their basal metabolic rates (an indicator of thyroid function). The thyroidectomized (Tx) rat is You correctly answered: a. BMR below 1600). BMR = 1650-1750). BMR = 1650-1750). The normal rat is You correctly answered: c. BMR above 1800) after the TSH injection. thyroid levels. hyperthyroid Judging from their basal metabolic rates (an indicator of thyroid function). hypothyroid 04/22/12 page 4 . BMR above 1800) after the thyroxine injection. BMR below 1600). thyroid levels. or hyperthryoid (high thyroid levels. categorize the rats as hypothyroid (low thyroid levels. or hyperthryoid (high thyroid levels. The hypophysectomized (Hypox) rat is You correctly answered: c. euthyroid ("good." or normal." or normal. BMR below 1600). hypothyroid Judging from their basal metabolic rates (an indicator of thyroid function). the normal rat c. BMR below 1600). categorize the rats as hypothyroid (low thyroid levels. categorize the rats as hypothyroid (low thyroid levels. BMR above 1800) after the PTU injection.The thyroidectomized (Tx) rat is You correctly answered: c. BMR below 1600). hyperthyroid Judging from their basal metabolic rates (an indicator of thyroid function). BMR above 1800) after the TSH injection. euthyroid ("good." or normal. BMR above 1800) after the PTU injection. categorize the rats as hypothyroid (low thyroid levels. or hyperthryoid (high thyroid levels. categorize the rats as hypothyroid (low thyroid levels. euthyroid ("good. or hyperthryoid (high thyroid levels. thyroid levels. hyperthyroid Which of the rats developed a goiter after injection with TSH (select all that apply)? You correctly answered: a. thyroid levels. euthyroid ("good." or normal. BMR = 1650-1750). euthyroid ("good. categorize the rats as hypothyroid (low thyroid levels. BMR = 1650-1750). euthyroid ("good. hyperthyroid Which of the rats developed a goiter after injection with thyroxine (select all that apply)? You correctly answered: d." or normal. BMR below 1600). None of the rats developed a goiter with this injection. BMR above 1800) after the TSH injection. The thyroidectomized (Tx) rat is You correctly answered: a. BMR = 1650-1750). The hypophysectomized (Hypox) rat is You correctly answered: c. thyroid levels. thyroid levels. or hyperthryoid (high thyroid levels." or normal. BMR = 1650-1750). hypothyroid Judging from their basal metabolic rates (an indicator of thyroid function). or hyperthryoid (high thyroid levels.

12 1542.3 432 378 384 510 474 462 480 384 468 378 378 378 BMR (ml O2/kg/hr) 1721. hypothyroid Which of the rats developed a goiter after injection with PTU (select all that apply)? You correctly answered: a.5 7.2 6. categorize the rats as hypothyroid (low thyroid levels. or hyperthryoid (high thyroid levels.35 2032 1935 1886 1912 1567 1910 1506 1543 1543 04/22/12 page 5 Palpation Injected No mass No mass No mass No mass No mass No mass Mass No mass Mass Mass No mass No mass none none none Thyroxine Thyroxine Thyroxine TSH TSH TSH PTU PTU PTU . The hypophysectomized (Hypox) rat is You correctly answered: a.3 6.3 6. euthyroid ("good. BMR = 1650-1750).8 6.7 8 6.9 7. the normal rat Experiment Data: Rat Weight (g) ml O2/min ml O2/hr Normal Tx Hypox Normal Tx Hypox Normal Tx Hypox Normal Tx Hypox 251 245 245 251 245 245 251 245 245 251 245 245 7.86 1567.4 8.4 7.Judging from their basal metabolic rates (an indicator of thyroid function)." or normal. thyroid levels. BMR below 1600). BMR above 1800) after the PTU injection.3 6.

Post-lab Quiz Results You scored 100% by answering 7 out of 7 questions correctly. 04/22/12 page 6 . How would you treat a thyroidectomized animal so that it functions like a "normal" animal? You correctly answered: c. 6. 1. Why did the normal rat develop a palpable goiter with the TSH injection? You correctly answered: c. The injection decreased the negative feedback mechanism on TSH. 2. Why didn't any of the rats develop a goiter after thyroxine injection? You correctly answered: a. Provide the animal T4 supplements. 5. An injection of propylthiouracil to an otherwise normal animal will cause which of the following? You correctly answered: b. decreased basal metabolic rate 3. An injection of thyroxine to an otherwise normal rat will cause which of the following? You correctly answered: b. The TSH receptors on the thyroid gland were excessively stimulated. Why did the normal rat develop a palpable goiter with the propylthiouracil injection? You correctly answered: d. what would be some expected symptoms? You correctly answered: d. hyperthyroidism 4. goiter development 7. In all cases. TSH levels were not elevated by the thyroxine injection. As a result of the missing hormone(s) in the hypophysectimized rat.

What was the effect of thyroxine injections on the thyroidectomized rat's BMR? How does the BMR in this case compare with the normal rat's BMR? Was the dose of thyroxine in the syringe too large. al no existir la glandula tiroidea que la sintetice 4.la rata normal se volvió hipertiroidica y no desarrollo bocio 6. If an animal has been thyroidectomized.en comparacion con la normal que si puede sintetizar tiroxina.creo que fue alta la inyeccion .tendrian una tasa metabolica baja . If an animal has been hypophysectomized. Part 2: Determining the Effect of Thyroxine on Metabolic Rate What was the effect of thyroxine injections on the normal rat's BMR? Your answer: la tiroxina aplicada . Why did the metabolic rates differ between the normal rat and the surgically altered rats? How well did the results compare with your prediction? Your answer: Las ratas no normales no podian sintetizar la hormona por lo tanto.En la rata tiroidectomizada la inyeccion de tiroxina elevo su tasa metabólica pero no desarrollo bocio porque no se elevo el nivel de TSH. por falta de las hormonas estimulantes 5. or just right? Your answer: 04/22/12 page 7 .Despues de inyectarse tiroxina a la tres ratas se incrmenta su tasa metabólica . incremento la tasa metabolica de 1721 a 2032 ml7o27hr .en todos por los resultados las ratas Tx e Hypox tubieron respectivamente 1543 y 1568 menores a 1722 de la normal 3.ninguna desarrolla bocio. Part 1: Determining the Basal Metabolic Rates Which rat had the fastest basal metabolic rate (BMR)? Your answer: la tasa metabolica mas alta se registro cuando se inyecto tiroxina a la rata normal. what hormone(s) would be missing in its blood? Your answer: faltaria la tiroxina o t4 .elevo la tasa metabolica . y como no tiene tiroides nunca podria darse el bocio.porque no hay incremento de TSH.Review Sheet Results 1. too small.se dio la retroalimentacion y no hubo bocio. se le suministro la hormona 2. or just right? Your answer: -En las ratas tiroidectomizada la inyeccion de tiroxina elevo su tasa metabolica de 1543 a 1935 -En la rata normal incrmento el nivel de tiroxina en sangre. . porque ademas de su produccion normal de esta. los resultados se compararon bien a mis predicciones . What was the effect of thyroxine injections on the hypophysectomized rat's BMR? How does the BMR in this case compare with the normal rat's BMR? Was the dose of thyroxine in the syringe too large. 7. what effect would you expect to see in the hormone levels in its body? Your answer: Sus niveles hormonales disminuyen. too small.

-En las ratas hipofisectomizada la inyeccion de tiroxina elevo su tasa metabolica de 1567 a 1886 -En la rata normal incrmento el nivel de tiroxina en sangre. 9. bocio . What was the effect of TSH injections on the hypophysectomized rat's BMR? How does the BMR in this case compare with the normal rat's BMR? Was the dose of TSH in the syringe too large.elevo la tasa metabolica .La tasa metabolica permanecio constante 10. What was the effect of TSH injections on the thyroidectomized rat's BMR? How does the BMR in this case compare with the normal rat's BMR? Why was this effect observed? Your answer: .se dio la retroalimentacion y no hubo bocio. no se presento mayor tiroxina por lo tanto no hubo incremento en la tasa metabólica del animal.la dosis fue alta en mi opinion 04/22/12 page 8 . or just right? Your answer: . . y como tiene hipofisis no puede estimular a la tiroides . En la rata hipofisectomizada la inyeccion de tiroxina elevo su tasa metabólica pero no desarrollo bocio porque no se elevo el nivel de TSH.En la rata de tiroidectomizada la aplicacion de TSH no tuvo efecto por carecer de la tirodes. llegando a tener hipertiroidismo y desarrolle bocio.generandole incremento de masa en la glandula . Part 3: Determining the Effect of TSH on Metabolic Rate What was the effect of thyroid-stimulating hormone (TSH) injections on the normal rat's BMR? Your answer: -En la rata normal la TSH aplicada estimulo la tiroides haciendoque se produzca tiroxina en exceso. que causo que la rata normal eleve su tasa metabolica de 1721 a 1912 pasando los 1800 . estimula producción de tiroxina en la tiroides esto elevo la tasa metabolica de la rata de 1567 a 1910 . too small.creo que fue alta la inyeccion 8.La TSH inyectada a la rata hipofisectomizada.