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Components and FactorAnalysis

**Multivariate Analysis often starts out with
**

data involving a substantial number of

correlated variables.

Principal Component Analysis (PCA) is a

dimension-reduction tool that can be used to

reduce a large set of variables to a small set

that still contains most of the information in

the large set.

• The first principal component accounts for as much of the variability in the data as possible. . • Principal components analysis is similar to another multivariate procedure called Factor Analysis.• Principal component analysis (PCA) is a mathematical procedure that transforms a number of (possibly) correlated variables into a (smaller) number of uncorrelated variables called principal components. They are often confused and many scientists do not understand the difference between the two methods or what types of analyses they are each best suited. and each succeeding component accounts for as much of the remaining variability as possible.

principal component analysis is performed on a square symmetric matrix. since these objects only differ in a global scaling factor.• Traditionally. or Correlation matrix (sums of squares and cross products from standardized data). . Covariance matrix (scaled sums of squares and cross products). • It can be a SSCP matrix (pure sums of squares and cross products). or if the units of measurement of the individual variates differ. • A correlation matrix is used if the variances of individual variates differ much. • The analysis results for objects of type SSCP and Covariance do not differ.

Geometry of Principal Components Campbell and Atchley .

Objectives of principal component analysis • PCA reduces attribute space from a larger number of variables to a smaller number of factors and as such is a "non-dependent" procedure (that is. The goal is dimension reduction and there is no guarantee that the dimensions are interpretable (a fact often not appreciated by (amateur) statisticians). •To select a subset of variables from a larger set. it does not assume a dependent variable is specified). • PCA is a dimensionality reduction or data compression method. . based on which original variables have the highest correlations with the principal component.

Explores underlying “latent” structure of data. Common factor analysis: Factor model explores a reduced correlation matrix. Model assumes variability partitionable into common and unique components Common variance: Variance shared with other variables in the factor analysis.Factor Analysis & Principal Components Definitions for Beginners Principal component analysis: Factor model in which the factors are based on summarizing the total variance. That is. unities are used in the diagonal of the correlation matrix computationally implying that all the variance is common or shared. Communality: Total amount of variance an original variable shares with all other variables included in the analysis. the latent root. With PCA. It conceptually represents that amount of variance accounted for by a factor. . Eigenvalue: Column sum of squared loadings for a factor. with specific and error variances excluded. Algorithm lacking underlying model. Specific or unique variance: Variance of each variable unique to that variable and not explained or associated with other variables in the factor analysis.. and the extracted factors are based only on the common variance.e. i. communalities (r2) are inserted on the diagonal of the correlation matrix.

.FAfB – continued . Factor matrix: Table displaying the factor loadings of all variables on each factor. Factor scores place each variable in a plane of multivariate variability. Factor score: Composite measure created for each observation on each factor extracted in the factor analysis. The factor weights are used in conjunction with the original variable values to calculate each observation's score. and the key to understanding the underlying nature of a particular factor. The factor scores are standardized to reflect a z-score. Factor loadings: Correlation between the original variables and the factors. Factors also represent the underlying dimensions (constructs) that summarize or account for the original set of observed variables. Squared factor loadings indicate what percentage of the variance in an original variable is explained by a factor.2 Sphericity test: Statistical test for the overall significance of all correlations within a correlation matrix Factor: Linear combination (variate) of the original variables.

PCA is far more common than PFA. Eigenvectors: Principal components (from PCA . and so on.Principal components analysis (PCA): PCA seeks a linear combination of variables such that the maximum variance is extracted from the variables.principal components analysis) reflect both common and unique variance of the variables and may be seen as a variance-focused approach seeking to reproduce both the total variable variance with all components and to reproduce the correlations. PCA analyzes total (common and unique) variance. This is called the principal axis method and results in orthogonal (uncorrelated) factors." The principal components are linear combinations of the original variables weighted by their contribution to explaining the variance in a particular orthogonal dimension . It then removes this variance and seeks a second linear combination which explains the maximum proportion of the remaining variance. and it is common to use "factors" interchangeably with "components. however.

A factor's eigenvalue may be computed as the sum of its squared factor loadings for all the variables. The eigenvalue for a given factor measures the variance in all the variables which is accounted for by that factor. Eigenvalues measure the amount of variation in the total sample accounted for by each factor.Eigenvalues: Also called characteristic roots. Note that the eigenvalues associated with the unrotated and rotated solution will differ. then it is contributing little to the explanation of variances in the variables and may be ignored as redundant with more important factors. If a factor has a low eigenvalue. though their total will be the same. The ratio of eigenvalues is the ratio of explanatory importance of the factors with respect to the variables. .

Analogous to Pearson's r.Factor loadings (factor or component coefficients) : The factor loadings. add the sum of the squared factor loadings for that factor (column) and divide by the number of variables. also called component loadings in PCA.) This is the same as dividing the factor's eigenvalue by the number of variables. the squared factor loading is the percent of variance in that variable explained by the factor. . To get the percent of variance in all the variables accounted for by each factor. (Note the number of variables equals the sum of their variances as the variance of a standardized variable is 1. are the correlation coefficients between the variables (rows) and factors (columns).

PC scores: Also called component scores in PCA. one takes the case's standardized score on each variable. multiplies by the corresponding factor loading of the variable for the given factor. these scores are the scores of each case (row) on each factor (column). To compute the factor score for a given case for a given factor. . and sums these products.

248257 0.1810705 0.8869 7 1.2171 0.5056124 0.0373 0.0416 0.498058 0.7538694 2.7876295 0.028 0.9149 .2362884 0.2196613 7.1469 0.Eigenvalues of the Covariance Matrix Eigenvalue Difference Proportion Cumulative 1 19.573 3 7.7225694 3.808 5 2.5139106 0.9349399 3.8496 6 2.7199 4 4.0119686 0.3559 0.3559 2 11.3555246 0.088 0.497092 0.

121 MDS24 -0.097 0.239 0.145 MDS44 0.188 -0.266 -0.038 -0.001 MDS19 0.007 0.228 0.130 -0.037 MDS29 0.146 0.174 0.089 0.073 0.172 -0.064 0.174 -0.084 -0.097 -0.003 0.054 0.059 0.105 -0.193 0.018 0.178 0.073 0.Portion of PCA Analysis of 54 Amino Acid Physio-Chemical Attributes Variable PCA1 PCA2 PCA3 PCA4 MDS3 0.196 0.104 0.005 MDS17 -0.020 -0.066 0.239 0.110 0.105 -0.242 PCA Coefficient .089 0.093 MDS16 0.106 MDS25 0.103 MDS30 -0.137 -0.117 0.128 -0.243 -0.161 MDS45 0.171 -0.156 -0.114 -0.031 MDS36 -0.076 MDS43 0.233 MDS32 0.061 0.203 -0.092 MDS31 0.

Introduction to Factor Analysis .

Factor analysis is a statistical procedure to identify interrelationships that exist among a large number of variables. then there is no underlying structure. factor analysis is used to search for a possible underlying structure in the variables. As an exploratory tool.e. Factor analysis can be used for exploratory or confirmatory purposes. In confirmatory research. The major difference between exploratory and confirmatory factor analysis is that researcher has formulated hypotheses about the underlying structure of the variables when using factor analysis for confirmatory purposes. i. as indicated by factor analysis. factor analysis doesn't have many statistical assumptions. If there are no correlations. . is to the expected structure. to identify how suites of variables are related. The only real assumption is presence of relatedness between the variables as represented by the correlation coefficient. As an exploratory procedure. the researcher evaluates how similar the actual structure of the data..

Steps in conducting a factor analysis There are five basic factor analysis steps: • data collection and generation of the correlation matrix • partition of variance into common and unique components (unique may include random error variability) • extraction of initial factor solution • rotation and interpretation • construction of scales or factor scores to use in further analyses .

unities are used in the diagonal of the correlation matrix computationally implying that all the variance is common or shared. Common factor analysis: Factor model explores a reduced correlation matrix. It conceptually represents that amount of variance accounted for by a factor. Specific or unique variance: Variance of each variable unique to that variable and not explained or associated with other variables in the factor analysis.Factor Analysis & Principal Components Definitions for Beginners Principal component analysis: Factor model in which the factors are based on the total variance. Communality: Total amount of variance an original variable shares with all other variables included in the analysis. That is. = the latent root. Common variance: Variance shared with other variables in the factor analysis. and the extracted factors are based only on the common variance. With PCA. communalities (r2) are inserted on the diagonal of the correlation matrix. . with specific and error variances excluded. Eigenvalue: Column sum of squared loadings for a factor.

The factor scores are standardized to according to a z-score. Factor matrix: Table displaying the factor loadings of all variables on each factor. and the key to understanding the nature of a particular factor. Factors also represent the underlying dimensions (constructs) that summarize or account for the original set of observed variables. Squared factor loadings indicate what percentage of the variance in an original variable is explained by a factor. Factor loadings: Correlation between the original variables and the factors.2 Sphericity test: Statistical test for the overall significance of all correlations within a correlation matrix Factor: Linear combination (variate) of the original variables. . The factor weights are used in conjunction with the original variable values to calculate each observation's score. Factor score: Composite measure created for each observation on each factor extracted in the factor analysis.FFB – continued .

FFB – continued . VARIMAX: One of the most popular orthogonal factor rotation methods. all other factors. Oblique factor rotation: Factor rotation computed so that the extracted factors are correlated. Rather than arbitrarily constraining the factor rotation to an orthogonal (90 degree angle) solution. the oblique solution identifies the extent to which each of the factors are correlated. Each factor is independent of. Orthogonal factor rotation: Factor rotation in which the factors are extracted so that their axes are maintained at 90 degrees. The correlation between teh factors is determined to be zero. or orthogonal to.3 Factor rotation: Process of manipulation or adjusting the factor axes to achieve a simpler and pragmatically more meaningful factor solution. .

Factor Rotation Unrotated Axes Rotated Axes Each variable lies somewhere in the plane formed by these two factors. which represent the correlation between the factor and the variable. can also be thought of as the variable's coordinates on this plane. Factor axes can be rotated to more closely correspond to the variables and therefore become more meaningful. In unrotated factor solution the Factor "axes" may not line up very well with the pattern of variables and the loadings may show no clear pattern. Relative relationships between variables are preserved. The factor loadings. The rotation can be either orthogonal or oblique .

18 .54 .47 .32 .30 Variable Factor 1 Factor 2 1 2 3 4 .40 .32 .02 . Rotation to simple structure generally simplifies the relationships among the variables and clarifies the interpretation of the factors.21 -.31 .19 .12 -.04 .39 -. .Rotation of Factors to “Simple Structure” Variable Factor 1 Factor 2 1 2 3 4 .06 Factors subjected to Varimax orthogonal rotation to simple structure. Simple structure attempts to clarify the relationships among variables by producing factors with either very high or very low coefficients and variables with high coefficients on only one variable.44 .

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158 -1.467 Asn 1.065 1.383 -0. Model amino acid and protein behaviors .385 0.340 -1. Decompose variances and covariances 2.205 Arg 1.726 2.096 0. Manova and discriminant analysis 3.Summary of Factor (not PCA) Analyses Factor matrix Correlation Matrix Amino acid attributes c1 r12 c2 r13 r23 c3 r1n r2n r3n cn Transform sequences C-MYC ALRDQIPELE L-MYC ALRDQVPTLA N-MYC TLRDHVPELV Ala Arg Asn Val λI X1 I X2 I X3 I Xn I λII X1 II X2 II X3 II Xn II λIII X1 III X2 III X3 III Xn III Factor scores Ala -0.

725 0.269 0.811 -0.183 0.657 -0.122 -0.023 -0.245 -0.024 -0.912 0.246 -0.224 0.439 -0.172 0.961 0.079 0.337 0.319 -0.048 Comm 0.297 0.092 0.956 0.073 0.224 0.021 -0.346 0.863 -0.780 F3 0.504 0.016 -0.104 -0.837 0.153 0.025 0.039 0.860 0.333 -0.095 0.388 -0.016 0.948 Factors describe latent structure of amino acid attributes .808 0.243 0.098 0.213 -0.714 0.047 -0.357 -0.218 -0.451 0.737 0.296 0.794 -0.292 0.982 0.112 0.969 0.416 0.091 -0.047 0.036 0.005 0.340 -0.159 0.034 0.225 0.394 -0.153 -0.708 0.194 0.152 0.108 -0.024 -0.338 F4 0.049 -0.946 0.693 -0.153 0.416 0.775 0.123 -0.790 0.905 0.005 0.060 -0.225 0.028 0.923 0.648 -0.433 -0.563 0.118 -0.939 0.185 0. Amino Acid Attributes Average non-bonded energy per atom Percentage of exposed residues Average accessible surface area Residue accessible surface area Number of hydrogen bond donors Polarity Hydrophilicity value Polar requirement Long range non-bonded energy Negative charge Positive charge Size Normalized relative frequency of bend Normalized frequency of beta-turn Molecular weight Relative mutability Normalized frequency of coil Average volume of buried residue Conformational parameter of beta-turn Residue volume Isoelectric point Propensity to form reverse turn Chou-Fasman coil conformation F1 1.085 -0.020 0.028 1.335 -0.928 0.915 0.730 0.237 0.955 0.019 -0.059 0.766 -0.079 -0.674 0.194 0.271 0.074 0.225 -0.172 -0.779 0.128 -0.189 -0.052 -0.809 0.967 -0.178 0.435 0.862 0.052 F5 -0.Portion of factor pattern matrix of 54 amino acid attributes.078 0.363 0.442 0.021 0.144 0.163 0.440 0.044 -0.024 1.027 0.965 0.201 F2 0.950 0.994 0.082 -0.

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