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ACID PHOSPHATASE (ACP

)

Continuous-spectrophotometric
SFBC

Instrument:
Principle of the method

SELECTRA-2

Acid phosphatase (ACP) catalyzes in acid medium the hydrolysis of the
phosphate group from α-naphtyl phosphate. The α-naphtol formed reacts with
a diazonium salt (Fast Red TR) originating a chromogen. The catalytic
concentration is determined from the rate of chromogen formation, measured
at 405 nm. Tartrate is used as a specific inhibitor of the prostatic fraction.

Samples

Reagent preparation
Working Reagent: Stopper the vial with the cap containing α-naphtyl
phosphate and press the red button until the solute falls into the vial. Add 10
mL of Reagent A1 (Total ACP) or 10 mL of Reagent A2 (Non Prostatic ACP).
Cap and shake until dissolved. Stable for 10 days at 2-8ºC.

Performance characteristics

Serum.
Acid phosphatase is unstable in serum. In acidfied serum is stable for 6 days at 2-8ºC.

Linearity: up to 150 U/L.
Interferences: Hemolysis and bilirubin interfere.

Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Delay, min. Time

ACP
Kinetic
405 nm
U/L
0
0 U/L
150 U/L
(...)
3
1
*
(...)
No

Prozone Check

No

Ref. Male Low
Ref. Male High
Ref. Female Low
Ref. Female High

0 U/L
10 U/L
0 U/L
10 U/L

Correlation Factor
Correlation Offset

1.000
0.000

ACP
No
25 mL
250 µ L
250 µ L

Linearity Limit
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor

10%
-0.050
3.000
-0.050
0.500
0.150
Yes
(...)
(...)

If the test is run without calibration enter
... Data entered by the operator
* assigned value of standard

FACTOR: 1480

20 µ L
10 µ L
289, 175 sec.

Version 0206

ALBUMIN

Spectrophotometric
BROMOCRESOL GREEN

Instrument:

SELECTRA-2

Principle of the method

Reagent preparation

Samples

Performance characteristics

Albumin in the sample reacts with bromocresol green in acid medium forming a Reagent is ready to use.
coloured complex that can be measured by spectrophotometry.
Serum, plasma.
Stable for 6 days at 2-8ºC.

-

Linearity: up to 70 g/L.
Interferences: Hemoglobin (1 g/L) and bilirubin (25 mg/dL) interfere.

Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Incubation Time

Albumin
Endpoint
620 nm
g/L
1
0 g/L
70 g/L
(...)
3
1
*
(...)
No
Albumin
No
25 mL
270 µ L
250 µ L
4 µL
3 µL
11.5 min.

Prozone Check

No

Ref. Male Low
Ref. Male High
Ref. Female Low
Ref. Female High

35 g/L
50 g/L
35 g/L
50 g/L

Correlation Factor
Correlation Offset

1.000
0.000

Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit

-0.100
3.000
-0.100
0.180

Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor

Yes
(...)
(...)

... Data entered by the operator
* assigned value of standard
Version 0207

ALKALINE PHOSPHATASE (ALP)

Continuous-spectrophotometric
DEA BUFFER

Instrument:

SELECTRA 2

Principle of the method

Reagent preparation

Samples

Performance characteristics

Alkaline phosphatase (ALP) catalyzes in alkaline medium the transfer of the
phosphate group from 4-nitrophenylphosphate to diethanolamine (DEA),
liberating 4-nitrophenol. The catalytic concentration is determined from the rate
of 4-nitrophenol formation, measured at 405 nm.
Serum, plasma.
Alkaline phosphatase in serum or plasma is stable for 7 days at 2-8ºC.
Heparin may be used as anticoagulant

Working Reagent: Dissolve the powder of a Reagent B vial with 20 mL of the
Reagent A bottle (if 10x20 mL size) or dissolve the contents of a Reagent B
vial with the entire volume of a Reagent A bottle (if 5x100 mL size).
Stable for 2 months at 2-8ºC.
-

Linearity: up to 690 U/L.
Interferences: Fluoride, oxalate, citrate and EDTA as anticoagulants
interfere. Hemolysis interferes due to the alkaline phosphatase content in
red cells.

Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Delay, min. Time

ALP DEA
Kinetic
405 nm
U/L
0
0 U/L
690 U/L
(...)
3
1
*
(...)
No

Prozone Check

No

Ref. Male Low
Ref. Male High
Ref. Female Low
Ref. Female High

90 U/L
280 U/L
90 U/L
280 U/L

Correlation Factor
Correlation Offset

1.000
0.000

ALP DEA
No
25 mL
250 µ L
250 µ L

Linearity Limit
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor

10%
-0.050
3.000
-0.050
0.950
0.150
Yes
(...)
(...)

If the test is run without calibration enter
... Data entered by the operator
* assigned value of standard

FACTOR: 2764

5 µL
3 µL
32, 175 sec.

Version 0012

ALKALINE PHOSPHATASE (ALP)

Continuous-spectrophotometric
AMP BUFFER (IFCC)

Instrument:

SELECTRA 2

Principle of the method

Reagent preparation

Samples

Performance characteristics

Alkaline phosphatase (ALP) catalyzes in alkaline medium the transfer of the
phosphate group from 4-nitrophenylphosphate to 2-amino-2-methyl-1-propanol
(AMP), liberating 4-nitrophenol. The catalytic concentration is determined from
the rate of 4-nitrophenol formation, measured at 405 nm.
Serum, plasma.
Alkaline phosphatase in serum or plasma is stable for 7 days at 2-8ºC.
Heparin may be used as anticoagulant

Working Reagent: Dissolve the powder of a Reagent B vial with 20 mL of the
Reagent A bottle (if 10x20 mL size) or dissolve the contents of a Reagent B
vial with the entire volume of a Reagent A bottle (if 5x100 mL size).
Stable for 2 months at 2-8ºC.
-

Linearity: up to 1200 U/L.
Interferences: Fluoride, oxalate, citrate and EDTA as anticoagulants
interfere. Hemolysis interferes due to the alkaline phosphatase content in
red cells.

Instrument settings
Name
Mode
Wavelength
Units
Decimals
Low Concentration
High Concentration
Calibrator Name
Repeat
Number
Concentration
Interval
Cut-off
MONO MODE
Name
Sample Blank
R1 Bottle
Normal Volume
Rerun Volume
Sample
Normal Volume
Rerun Volume
Delay, min. Time

ALP IFCC
Kinetic
405 nm
U/L
0
0 U/L
1200 U/L
(...)
3
1
*
(...)
No

Prozone Check

No

Ref. Male Low
Ref. Male High
Ref. Female Low
Ref. Female High

26 U/L
117 U/L
26 U/L
117 U/L

Correlation Factor
Correlation Offset

1.000
0.000

ALP IFCC
No
25 mL
250 µ L
250 µ L

Linearity Limit
Low Absorbance
High Absorbance
R. Abs. Low Limit
R. Abs. High Limit
R. Abs. Deviation
Reagent Blank
Cal. Low Limit
Cal. High Limit
Factor

10%
-0.050
3.000
-0.050
0.950
0.150
Yes
(...)
(...)

If the test is run without calibration enter
... Data entered by the operator
* assigned value of standard

FACTOR: 2764

5 µL
3 µL
32, 175 sec.

Version 0012

Time ALT Kinetic 340 nm U/L 0 0 U/L 500 U/L (... Abs. Male High Ref. DUAL MODE. Deviation Reagent Blank Cal.500 0. Stable for 2 months at 2-8ºC. Deviation Reagent Blank Cal. min.. forming pyruvate and glutamate. measured at 340 nm. The catalytic concentration is determined from the rate of decrease of NADH. Female High 0 U/L 41 U/L 0 U/L 41 U/L Correlation Factor Correlation Offset 1. Alanine aminotransferase in serum is stable for 7 days at 2-8ºC. Female Low Ref...) Linearity Limit Low Absorbance High Absorbance R.) 3 1 * (. Low Limit Cal. High Limit Factor 10% 0. Time DUAL MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume R2 Bottle Normal Volume Rerun Volume Predilution Slope Blank Delay.. min.000 ALT No 25 mL 250 µ L 250 µ L Linearity Limit Low Absorbance High Absorbance R. Low Limit R.. 132 sec. High Limit R.100 Yes (.) No Prozone Check No Ref.) If the test is run without calibration enter . reducing the linearity of the method. Mix gently.150 Yes (..800 2. Linearity: Up to 500 U/L. Abs. High Limit Factor 10% 0...ALANINE AMINOTRANSFERASE (ALT) Continuous-spectrophotometric IFCC Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Alanine aminotransferase (ALT or GPT) catalyzes the transfer of the amino group from alanine to 2-oxoglutarate. Abs. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Delay.) (..200 0. 176 sec. Data entered by the operator * assigned value of standard FACTOR: -3466 12 µ L 6 µL 70..) (. High Limit R.. Reagent 2: Use the Reagent B.200 0. Abs. by means of the lactate dehydrogenase (LDH) coupled reaction.. Abs.800 2.000 0. Version 0012 . Low Limit R. Male Low Ref.800 2. Serum. Abs..800 2.. MONO MODE. Low Limit Cal. - Interferences: High pyruvate in the sample will consume NADH during the delay time before measurements.500 0.Reagent 1: Use the Reagent A. ALT No 25 mL 225 µ L 225 µ L 12 µ L 6 µL 25 mL 25 µ L 25 µ L No No 77.Working Reagent: Pour the contents of the Reagent B into the Reagent A bottle.

α-Amylase in serum. (CNP-G3) to 2-chloro-4-nitrophenol (CNP). Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Delay. Data entered by the operator * assigned value of standard FACTOR: 3292 5 µL 3 µL 32.. Interferences: Fluoride. Serum. Female High 0 U/L 60 U/L 0 U/L 60 U/L Correlation Factor Correlation Offset 1. Abs..) 3 1 * (. Abs. Low Limit Cal.. measured at 405 nm. Male Low Ref..) (. citrate and EDTA as anticoagulants interfere. min. plasma or urine is stable for 5 days at 2-8ºC. Low Limit R.... urine. Heparin may be used as anticoagulant - Linearity: up to 1300 U/L (serum) or 2600 U/L (urine).150 Yes (. High Limit R. oxalate.. plasma. Deviation Reagent Blank Cal.) If the test is run without calibration enter .050 3.250 0. The catalytic concentration is determined from the rate of 2-chloro-4-nitrophenol formation. Time AMYL DIRECT Kinetic 405 nm U/L 0 0 U/L 1300 U/L (.000 -0. No 25 mL 250 µ L 250 µ L Linearity Limit Low Absorbance High Absorbance R. High Limit Factor 10% -0.) No Prozone Check No Ref. 175 sec.. Abs. Male High Ref. Version 0012 ..000 AMYLASE DIR. Female Low Ref.α -AMYLASE Continuous-spectrophotometric DIRECT SUBSTRATE Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics α-Amylase catalyzes the hydrolysis of 2-chloro-4-nitrophenyl-maltotrioside Reagent is ready to be used.050 0.000 0.

. The catalytic concentration is determined from the rate of 4-nitrophenol formation. High Limit R. Female High 28 U/L 100 U/L 28 U/L 100 U/L Correlation Factor Correlation Offset 1.100 3. Stable for 20 days at 2-8ºC. Male High Ref.. Use heparin or EDTA as anticoagulant. Other drugs and substances may interfere. Interferences: Lipemia (triglycerides 10 g/L) and blirubin (20 mg/dL) do not interfere. Low Limit Cal.) If the test is run without calibration enter . plasma.000 -0. Low Limit R...000 AMYLASE No 25 mL 250 µ L 250 µ L Linearity Limit Low Absorbance High Absorbance R. urine.. min... α-Amylase in urine is stable for 1 month at 2-8ºC if pH is adjusted to approximately 7 before storage. 195 sec. Version 0204 . Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Delay..α -AMYLASE Continuous-spectrophotometric IFCC Instrument: SELECTRA-2 Principle of the method Reagent preparation α-Amylase catalyzes the hydrolysis of 4-nitrophenyl-maltoheptaosideethylidene to smaller oligosacharides which are hydrolyzed by α-glucosidase liberating 4-nitrophenol. Other volumes can be prepared in the proportion: 4 mL Reagent A + 1 mL Reagent B. High Limit Factor 10% -0. Hemoglobin (10 g/L) interfere.) 3 1 * (. Abs.) No Prozone Check No Ref. Linearity: up to 1300 U/L (serum) or 2600 U/L (urine)..000 0..300 Yes (. Abs.100 3. Data entered by the operator * assigned value of standard FACTOR: 3042 8 µL 6 µL 129. Female Low Ref.) (. Performance characteristics Serum.000 0. Deviation Reagent Blank Cal. Mix gently. Time AMYL Kinetic 405 nm U/L 0 0 U/L 1300 U/L (. measured at 405 nm. α-Amylase in serum or plasma is stable for 1 month at 2-8ºC. Abs. Male Low Ref. Samples Working Reagent: Pour the contents of the Reagent B into the Reagent A bottle.

200 0. 132 sec. Reagent 2: Use the Reagent B. Serum..500 0. Male Low Ref. The catalytic concentration is determined from the rate of decrease of NADH.150 Yes (.500 0.. Linearity: Up to 500 U/L.. Abs. High Limit Factor 10% 0. High Limit Factor 10% 0.) If the test is run without calibration enter . Low Limit Cal... 176 sec. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Delay. Aspartate aminotransferase in serum is stable for 7 days at 2-8ºC. Low Limit R.800 2.) (. Version 0012 .200 0..) (. MONO MODE.. Time DUAL MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume R2 Bottle Normal Volume Rerun Volume Predilution Slope Blank Delay. min..800 2.000 AST No 25 mL 250 µ L 250 µ L Linearity Limit Low Absorbance High Absorbance R. High Limit R. measured at 340 nm..) Linearity Limit Low Absorbance High Absorbance R.. High Limit R. Female Low Ref. Mix gently. Abs. Deviation Reagent Blank Cal. Data entered by the operator * assigned value of standard FACTOR: -3465 12 µ L 6 µL 70.100 Yes (. Abs. forming oxalacetate and glutamate.ASPARTATE AMINOTRANSFERASE (AST) Continuous-spectrophotometric IFCC Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Aspartate aminotransferase (AST or GOT) catalyzes the transfer of the amino group from aspartate to 2-oxoglutarate. by means of the malate dehydrogenase (MDH) coupled reaction..) No Prozone Check No Ref..000 0. Time AST Kinetic 340 nm U/L 0 0 U/L 500 U/L (. Stable for 2 months at 2-8ºC. - Interferences: High pyruvate in the sample will consume NADH during the delay time before measurements..Reagent 1: Use the Reagent A. DUAL MODE... Low Limit R.800 2. AST No 25 mL 225 µ L 225 µ L 12 µ L 6 µL 25 mL 25 µ L 25 µ L No No 77. Abs. reducing the linearity of the method. Low Limit Cal..800 2. Abs. Abs. Deviation Reagent Blank Cal.) 3 1 * (. Female High 0 U/L 42 U/L 0 U/L 42 U/L Correlation Factor Correlation Offset 1. min.Working Reagent: Pour the contents of the Reagent B into the Reagent A bottle. Male High Ref.

Low Limit Cal...100 0. Low Limit R... Both A-T bottle.000 0. Female High 0. Abs. The terms “direct” and “total” refer to the reaction characteristics of serum bilirubin in the absence or presence of solubilizing (accelerating) reagents.5 min. Female Low Ref.) No T-BILIRUBIN No 25 mL 250 µ L 250 µ L 25 µ L 15 µ L 11. Data entered by the operator * assigned value of standard Version 0012 . Stable for 20 days at 2-8 ºC and protected from light.00 mg/dL 1. Prozone Check No Ref.. and are only approximately equivalent to the conjugated and unconjugated fractions.) ..TOTAL BILIRUBIN Spectrophotometric DIAZOTIZED SULFANILIC Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Total bilirubin in the sample reacts with diazotized sulfanilic in acid medium Working Reagent: Transfer the contents of one Reagent B vial into a Reagent forming a coloured complex that can be measured by spectrophotometry. Abs.100 3.... Mix thoroughly. High Limit -0.00 mg/dL 1. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Incubation Time Bilirubin Endpoint 546 nm mg/dL 2 0 mg/dL 15 mg/dL (.000 -0.10 mg/dL Correlation Factor Correlation Offset 1. couple with diazo in the presence of cetrimide..000 Low Absorbance High Absorbance R. Male High Ref.10 mg/dL 0. direct (conjugated with glucuronate) and indirect (unconjugated) bilirubin Stable for 20 days at 2-8ºC. Male Low Ref. High Limit Factor Yes (.150 Reagent Blank Cal.) (.) 3 1 * (. - Linearity: Up to 15 mg/dL. Serum.

.7 mg/dL 9. heparinized plasma.) 3 1 * (.. Prozone Check No Ref.100 2.CALCIUM Spectrophotometric ARSENAZO III Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Calcium in the sample reacts with arsenazo III forming a coloured complex that Reagent is ready to be used..) No CALCIUM No 25 mL 300 µ L 300 µ L 4 µL 2 µL 11.. magnesium (10 mg/dL) and phosphate (20 mg/dL) do not interfere. Anticoagulants other than heparin should not be used.000 0. Low Limit Cal. Male Low Ref. Interferences: Hemoglobin (1. can be measured by spectrophotometry.000 Reagent Blank Cal..0 mg/dL 10. Abs. High Limit Factor Yes (.. Calcium in serum or plasma is stable for 10 days at 2-8 ºC. Female Low Ref. urine..5 g/L). Serum. High Limit -0.000 -0. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Incubation Time Calcium Endpoint 620 nm mg/dL 2 0 mg/dL 18 mg/dL (.. Abs.) (. Data entered by the operator * assigned value of standard Version 0012 . Low Limit R.5 min. - Linearity: Up to 18 mg/dL.) . Female High 9..7 mg/dL Correlation Factor Correlation Offset 1..0 mg/dL 10.100 3. Male High Ref.000 Low Absorbance High Absorbance R. bilirubin (20 mg/dL).

bilirubin (20 mg/dL). Anticoagulants other than heparin should not be used.. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Incubation Time Calcium Endpoint 620 nm mg/dL 2 0 mg/dL 15 mg/dL (. Female Low Ref...) . Interferences: Hemoglobin (1.0 mg/dL 10..0 mg/dL 10.CALCIUM Spectrophotometric METHYLTHYMOL BLUE Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Calcium in the sample reacts with methylthymol blue in alkaline medium Working Reagent: Mix equal volumes of Reagent A and Reagent B. Calcium in serum or plasma is stable for 10 days at 2-8 ºC. thoroughly.. High Limit Factor Yes (. Female High 9. magnesium (10 mg/dL) and phosphate (20 mg/dL) do not interfere. Hydroxyquinoline is included in the reagent to remove magnesium interference. Male High Ref.5 g/L).000 0. Serum. Male Low Ref.100 0. Low Limit R.. Low Limit Cal.. Stable for 2 days at 2-8ºC. urine. Mix forming a coloured complex that can be measured by spectrophotometry. High Limit -0. Prozone Check No Ref..000 -0. Abs.5 min.) No CALCIUM No 25 mL 300 µ L 300 µ L 3 µL 2 µL 11. heparinized plasma.000 Low Absorbance High Absorbance R.) 3 1 * (. Abs. - Linearity: Up to 15 mg/dL.7 mg/dL 9. Data entered by the operator * assigned value of standard Version 0012 .700 Reagent Blank Cal.7 mg/dL Correlation Factor Correlation Offset 1.100 3...) (.

Version 0203 . Female High 2150 U/L 4950 U/L Correlation Factor Correlation Offset 1.950 0.) If the test is run without calibration enter . Time Cholinesterase Kinetic 505 nm U/L 0 0 U/L 6500 U/L (... - Linearity: up to 6500 U/L. heparinized plasma or EDTA plasma..150 Yes (.000 0.. Female Low Ref.) (...) 3 1 * (. by means of the choline and peroxidase coupled reactions..050 3. Low Limit Cal.000 Cholinesterase No 25 mL 300 µ L 250 µ L Linearity Limit Low Absorbance High Absorbance R. Abs. measured at 500 nm. High Limit Factor 10% -0. High Limit R..000 -0. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Delay. Low Limit R. Abs. Male High Ref. Abs. Serum. The catalytic concentration is determined from the rate of quinoneimine formation.. Stable for 10 days at 2-8ºC. Data entered by the operator * assigned value of standard FACTOR: 28000 2 µL 2 µL 188.CHOLINESTERASE Continuous-spectrophotometric BENZOYLCHOLINE Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Cholinesterase (CHE) catalyzes the hydrolysis of benzoylcholine to choline and benzoic acid. Male Low Ref. Cholinesterase in serum or plasma is stable for 7 days at 2-8ºC. 175 sec.050 0.. Swirl gently. min. Deviation Reagent Blank Cal.) No Prozone Check No Ref. Working Reagent: Reconstitute the contents of a Reagent B vial with 3 mL (if 20x3 mL size). 15 mL (if 10x15 mL size) or 50 mL (if 4x50 mL size) of Reagent A.

) No Cholesterol No 25 mL 300 µ L 300 µ L 3 µL 2 µL 11..25 mmol/L) interfere.) .. Abs. a coloured complex that can be measured by spectrophotometry. Stable for 7 days at 2-8ºC. Low Limit R. Lipemia does not affect results. Data entered by the operator * assigned value of standard Version 0012 .) (. Heparin..100 0.. ascorbic acid (0. Male High Ref.5 min.000 -0. High Limit Factor Yes (. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Incubation Time Cholesterol Endpoint 505 nm mg/dL 0 0 mg/dL 1000 mg/dL (. EDTA. Male Low Ref.3 mmol/L) and bilirubin (0.....) 3 1 * (.100 3.CHOLESTEROL Enzymatic-spectrophotometric CHOLESTEROL OXIDASE/PEROXIDASE Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Free and esterified cholesterol in the sample originates. Female High 123 mg/dL 270 mg/dL 123 mg/dL 243 mg/dL Correlation Factor Correlation Offset 1. Linearity: Up to 1000 mg/dL. oxalate and fluoride may be used as anticoagulants.150 Reagent Blank Cal. Low Limit Cal. Abs. High Limit -0.. by means of some Reagent is ready to be used coupled reactions.000 Low Absorbance High Absorbance R. Female Low Ref. - Interferences: Hemoglobin (3 g/L).000 0. Prozone Check No Ref. Serum or plasma..

.. High Limit R.000 0.) (. of the hexokinase (HK) and glucose-6-phosphate coupled reactions. Female Low Ref. Version 0101 .050 0. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Delay. to form ATP and creatine.150 Yes (. Deviation Reagent Blank Cal.500 0.000 -0.. - Linearity: up to 730 U/L. High Limit Factor 10% -0. Female High 38 U/L 174 U/L 26 U/L 140 U/L Correlation Factor Correlation Offset 1. in the presence of Working Reagent: Empty the contents of a Reagent B bottle into a Reagent A creatine phosphate.) If the test is run without calibration enter .) No Prozone Check No Ref. min.. measured at 340 nm.) 3 1 * (. Swirl gently.. Creatine kinase in serum is stable for 7 days at 2-8ºC. Serum. Low Limit Cal. Male High Ref..050 3.. Male Low Ref.000 CK No 25 mL 250 µ L 250 µ L Linearity Limit Low Absorbance High Absorbance R. Abs. The catalytic concentration is bottle. Abs... Data entered by the operator * assigned value of standard FACTOR: 3465 12 µ L 6 µL 188. Abs. by means Stable for 15 days at 2-8ºC. 175 sec.. Low Limit R. Time CK Kinetic 340 nm U/L 0 0 U/L 730 U/L (. determined from the rate of NADPH formation.CREATINE KINASE (CK) Continuous-spectrophotometric IFCC Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Creatine kinase (CK) catalyzes the phosphorylation of ADP.

1 g/L). Abs. 103 sec. The complex formation rate is measured in a short period to Reagent 2: Use the Reagent B. Heparin. plasma. Female Low Ref. Data entered by the operator * assigned value of standard Version 0012 . Male Low Ref.350 0...CREATININE Kinetic-spectrophotometric ALKALINE PICRATE Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Creatinine in the sample reacts with picrate in alkaline medium forming a Reagent 1: Use the Reagent A..100 3. High Limit R. Abs.. two Creatinine Twopoint 505 nm mg/dL 1 0 mg/dL 20 mg/dL (.. - Interferences: Hemoglobin (0.000 0.. Deviation Reagent Blank Cal.000 Low Absorbance High Absorbance R. Low Limit R. Low Limit Cal.) No Creatinine No 25 mL 190 µ L 190 µ L 25 µ L 12 µ L 25 mL 65 µ L 65 µ L No No 24. Serum. Prozone Check No Ref. urine. protein and ketonic bodies do not interfere.) (. coloured complex.5 mg/dL 0. oxalate and fluoride may be used as anticoagulants. EDTA. Linearity: Up to 20 mg/dL (serum or plasma).6 mg/dL 1. bilirubin (10 mg/dL). Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off DUAL MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume R2 Bottle Normal Volume Rerun Volume Predilution Slope Blank Point one.1 mg/dL 0. Creatinine in serum or plasma is stable for 24 hours at 2-8ºC. Abs.000 -0...9 mg/dL Correlation Factor Correlation Offset 1..) 3 1 * (..100 Yes (. Male High Ref. avoid interferences.) . Female High 0.100 0. High Limit Factor -0.

Female Low Ref.bottle. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Delay... Male Low Ref.. High Limit R. Deviation Reagent Blank Cal. Male High Ref..050 0. Serum.. carboxy-4-nitroaniline. Female High 15 U/L 86 U/L 10 U/L 40 U/L Correlation Factor Correlation Offset 1.) (.000 -0. High Limit Factor 10% -0. Version 0207 . min. 175 sec. liberating 3..GAMMA-GLUTAMYLTRANSFERASE (γγ -GT) Continuous-spectrophotometric IFCC Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Gamma-glutamyltansferase γ( -GT) catalyzes the transfer of the γ-glutamyl Working Reagent: Pour the contents of the Reagent B into the Reagent A group from γ-glutamyl-3-carboxy-4-nitroanilide to glycylglycine. Low Limit R. - Linearity: up to 300 U/L.000 0. Time GGT Kinetic 405 nm U/L 0 0 U/L 300 U/L (. of 3-carboxy-4-nitroaniline formation.000 GGT No 25 mL 250 µ L 250 µ L Linearity Limit Low Absorbance High Absorbance R. The catalytic concentration is determined from the rate Stable for 2 months at 2-8 ºC. Gamma-glutamyltransferase in serum is stable for 5 days at 2-8 ºC.) If the test is run without calibration enter . Low Limit Cal. Mix gently. Data entered by the operator * assigned value of standard FACTOR: 1111 25 µ L 12 µ L 32...900 0. Abs.. Abs.) No Prozone Check No Ref.050 3.) 3 1 * (.150 Yes (. Abs..

Prozone Check No Ref. Low Limit Cal. Female High 76 mg/dL 110 mg/dL 76 mg/dL 110 mg/dL Correlation Factor Correlation Offset 1. Stable for 7 days at 2-8ºC.) No Glucose No 25 mL 300 µ L 300 µ L 3 µL 2 µL 11. ascorbic acid (10 mg/dL) and bilirubin (15 mg/dL) interfere..150 Reagent Blank Cal.) 3 1 * (. Male High Ref. Abs. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Incubation Time Glucose Endpoint 505 nm mg/dL 0 0 mg/dL 500 mg/dL (. Linearity: Up to 500 mg/dL.3 g/L).000 0. EDTA.. a Reagent is ready to be used coloured complex that can be measured by spectrophotometry. Moderate lipemia does not affect the results.. Male Low Ref..100 3. Heparin. Data entered by the operator * assigned value of standard Version 0012 ..GLUCOSE Enzymatic-spectrophotometric GLUCOSE OXIDASE/PEROXIDASE Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Glucose in the sample originates. oxalate and fluoride may be used as anticoagulants.000 Low Absorbance High Absorbance R.000 -0.100 0. High Limit -0.5 min. Female Low Ref.) (.. Low Limit R. Abs.. by means of some coupled reactions.) .. - - Interferences: Hemoglobin (0. High Limit Factor Yes (. Serum or plasma...

100 0.. Precipitation Procedure: 1. Interferences: Hemoglobin (1 g/L). Stable for 7 days at 2-8ºC. High Limit Factor Yes (.5 mL 2.) 3 1 * (. bilirubin (10 mg/d/L) and acid ascorbic (0.. Prozone Check No Ref.5 min. High Limit -0. Female High 30 mg/dL 60 mg/dL 40 mg/dL 70 mg/dL Correlation Factor Correlation Offset 1. Serum or plasma. Mix thoroughly and let stand for 10 minutes at room temperature. Abs. Centrifuge at a minimum of 4000 r.m.000 Low Absorbance High Absorbance R.100 3.. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Incubation Time HDL-C Endpoint 505 nm mg/dL 0 0 mg/dL 200 mg/dL (.p. for 10 minutes.) . sample precipitate with phosphotungstate and magnesium ions. EDTA. Pipette into labelled centrifuge tubes: Sample 0. Abs. Low Limit R.. 4. Heparin.000 0.. - Linearity: up to 200 mg/dL. The HDL cholesterol is then spectrophotometrically measured by means of some coupled reactions.HDL CHOLESTEROL Precipitation/Enzymatic-spectrophotometric PHOSPHOTUNGSTATE/Mg 2+-CHOLESTEROL OXIDASE/PEROXIDASE Instrument: SELECTRA-2 Principle of the method Reagent preparation Samples Performance characteristics Very low density lipoproteins (VLDL) and low density lipoproteins (LDL) in the Reagent B is ready to be used. The supernatant contains high density lipoproteins (HDL).. oxalate and fluoride may be used as anticoagulants.000 -0.1 mmol/L) interfere. Carefully collect the supernatant..200 Reagent Blank Cal.2 mL Reagent A 0.. Male Low Ref. Male High Ref. Low Limit Cal. Data entered by the operator * assigned value of standard Version 0206 .. Female Low Ref.) (.) No HDL-C No 25 mL 260 µ L 260 µ L 13 µ L 2 µL 11. 3..

100 3. Abs.000 -0. High Limit R.000 0. Prozone Check No Ref.100 No (.. Abs. Female High 70 µ g/dL 155 µ g/dL 55 µ g/dL 140 µ g/dL Correlation Factor Correlation Offset 1. High Limit Factor -0.5 min..IRON Spectrophotometric CHROMAZUROL B Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Ferric ions in the sample react with chromazurol B and Reagent is ready to be used cetyltrimethylamoniumbromide (CTAB) forming a coloured complex that can be measured by spectrophotometry..100 3. Male Low Ref. Data entered by the operator * assigned value of standard Version 0012 . Serum or heparinized plasma.) (.. Deviation Reagent Blank Cal. Low Limit Cal.) 3 1 * (. Low Limit R.) No Iron No 25 mL 250 µ L 250 µ L 12 µ L 12 µ L 11. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Incubation Time Iron Endpoint 620 nm µ g/dL 0 0 µ g/dL 500 µ g/dL (. Female Low Ref.. Linearity: Up to 500 µg/dL. Abs.. Stable for 7 days at 2-8ºC.) ..000 0...000 Low Absorbance High Absorbance R. - Interferences: Do not use hemolyzed sera.. Male High Ref.

IRON Spectrophotometric FERROZINE Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Transferrin-bound ferric ions in the sample are released by guanidinium and Working Reagent: Transfer the contents of one Reagent B vial into a Reagent reduced to ferrous by means of hydroxylamine.5 min..) . Linearity: Up to 1000 µg/dL. Low Limit Cal.. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Incubation Time Iron Ferrozine Endpoint 546 nm µ g/dL 0 0 µ g/dL 1000 µ g/dL (.000 0. Female Low Ref.. Male Low Ref. Stable for 7 days at 2-8ºC.000 -0. Female High 70 µ g/dL 155 µ g/dL 55 µ g/dL 140 µ g/dL Correlation Factor Correlation Offset 1.150 Reagent Blank Cal. Mix thoroughly.000 Low Absorbance High Absorbance R.) (. Ferrous ions react with A bottle.100 3. Data entered by the operator * assigned value of standard Version 0012 ... Abs.) 3 1 * (... Serum or heparinized plasma. - Interferences: Do not use hemolyzed sera. Male High Ref. High Limit Factor Yes (.) No Iron Ferrozine Yes 25 mL 220 µ L 220 µ L 30 µ L 15 µ L 11. spectrophotometry. Prozone Check No Ref. High Limit -0.. Low Limit R. ferrozine forming a coloured complex that can be measured by Stable for 6 months at 2-8ºC.100 0. Abs...

The catalytic concentration is determined bottle. Female High 207 U/L 414 U/L 207 U/L 414 U/L Correlation Factor Correlation Offset 1. Lactate dehydrogenase in serum or plasma is stable for 24 hours at 2-8ºC. Low Limit Cal. Abs. to form lactate and NAD+. Stable for 2 months at 2-8ºC. min. Low Limit R. Linearity: Up to 1500 U/L.050 3..000 -0. Male Low Ref.000 0. Abs. measured at 340 nm. Heparin may be used as anticoagulant. Mix gently. High Limit R. from the rate of decrease of NADH. Serum or plasma....LACTATE DEHYDROGENASE (LD/LDH) Continuous-spectrophotometric PYRUVATE Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Lactate dehydrognase (LD or LDH) catalyzes the reduction of pyruvate by Working Reagent: Pour the contents of the Reagent B into the Reagent A NADH...) 3 1 * (.) If the test is run without calibration enter .050 2.. Male High Ref.) (. Female Low Ref.. - Interferences: Hemolysis interferes due dehydrogenase concentration in red cells. 175 sec.. Deviation Reagent Blank Cal.150 Yes (. Data entered by the operator * assigned value of standard FACTOR: -8095 5 µL 3 µL 32.) No Prozone Check No Ref. High Limit Factor 10% -0. to the Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Delay. Version 0203 high lactate ..000 0. Abs. Time LDH Kinetic 340 nm U/L 0 0 U/L 1200 U/L (.000 LDH No 25 mL 250 µ L 250 µ L Linearity Limit Low Absorbance High Absorbance R.

Data entered by the operator * assigned value of standard Version 0206 . Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Incubation Time LDL-Cholesterol Endpoint 505 nm mg/dL 0 0 mg/dL 500 mg/dL (.000 -0.. Stable for 24 hours at 2-8ºC.. Prozone Check Ref.m. Female Low Ref.LDL CHOLESTEROL Precipitation/Enzymatic-spectrophotometric POLIVINYL SULPHATE-CHOLESTEROL OXIDASE/PEROXIDASE Instrument: SELECTRA-2 Principle of the method Reagent preparation Samples Performance characteristics Sample preparation - Low density lipoproteins (LDL) in the sample precipitate with polivinyl sulphate. Low Limit R.. bilirubin (10 mg/d/L) and acid ascorbic (0.5 min.1 mL Reagent B 2.Mix thoroughly and let stand for 15 minutes at room temperature 3..100 3. Serum.. The LDL cholesterol is then spectrophotometrically measured by means of some coupled reactions. Male High Ref. Female High No 150 mg/dL 150 mg/dL Correlation Factor Correlation Offset 1. for 15 minutes 4..000 Low Absorbance High Absorbance R. High Limit Factor Yes (. LDL cholesterol concentration is calculated by substracting cholestrol values in serum from supernatant values after being precipitated. High Limit -0.Centrifuge at a minimum of 4000 r..p.000 0. Interferences: Hemoglobin (1 g/L)... Male Low Ref.100 0.2 mL Sample + 0. Low Limit Cal. Abs.) (. Abs..) No LDL-Cholesterol No 25 mL 300 µ L 300 µ L 6 µL 3 µL 11.Carefully collect the supernatant Linearity: up to 500 mg/dL. Reagent is ready to be used. The supernatant contains low density lipoproteins (LDL)..1 mmol/L) interfere... Precipitation: 1.150 Reagent Blank Cal.) .) 3 1 * (.Pipette into labelled centrifuge tubes: 0..

Anticoagulants other than heparin should not be used.) 3 1 * (. Magnesium in serum or plasma is stable for 10 days at 2-8ºC. Abs. Female Low Ref. calcium (20 mg/dL) and bilirubin (20 mg/dL) do not interfere.100 3. Female High 1.. heparinized plasma.0 mg/dL 4. Abs.700 Reagent Blank Cal. Data entered by the operator * assigned value of standard Version 0109 ..000 0.. Serum.8 mg/dL 2.) . Prozone Check No Ref.1 mg/dL 1..5 g/L).5 min.000 -0. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Incubation Time MAGNESIUM Endpoint 505 nm mg/dL 1 0.. EGTA is included in the reagent to remove calcium interference.) (. coloured complex that can be measured by spectrophotometry.100 0..8 mg/dL 2. Linearity: Up to 4 mg/dL. - Interferences: Hemoglobin (1. Low Limit R..MAGNESIUM Spectrophotometric CALMAGITE Instrument: SELECTRA-2 Principle of the method Reagent preparation Samples Performance characteristics Magnesium in the sample reacts with calmagite in alkaline medium forming a Reagent is ready to be used.1 mg/dL Correlation Factor Correlation Offset 1. Low Limit Cal.) No MAGNESIUM No 25 mL 250 µ L 250 µ L 3 µL 2 µL 11. Male Low Ref. High Limit Factor Yes (...0 mg/dL (..000 Low Absorbance High Absorbance R. Male High Ref. High Limit -0.

100 3.000 0.5 min.000 Low Absorbance High Absorbance R. Abs. Low Limit R.70 mg/dL 4. Interferences: Do not use hemolyzed sera.. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Incubation Time Phosphorus Endpoint 340 nm mg/dL 2 0 mg/dL 20 mg/dL (..PHOSPHORUS Spectrophotometric PHOSPHOMOLYBDATE/UV Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Inorganic phosphorus in the sample reacts with molybdate in acid medium Working Reagent: Mix 35 mL Reagent A + 15 mL Reagent B.. Low Limit Cal... plasma.) . High Limit Factor Yes (. High Limit -0. Linearity: Up to 20 mg/dL.000 -0...) 3 1 * (.) (. Male High Ref. Female High 2.50 mg/dL 2.500 Reagent Blank Cal. Abs.50 mg/dL Correlation Factor Correlation Offset 1.. Data entered by the operator * assigned value of standard Version 0012 .70 mg/dL 4.) No Phosphorus Yes 25 mL 300 µ L 300 µ L 3 µL 2 µL 11. Phosphorus in serum or plasma is stable for 7 days at 2-8ºC. Female Low Ref. EDTA and fluoride may be used as anticoagulants. forming a phosphomolybdate complex that can be measured by Stable for 12 months at 15-30ºC. urine. spectrophotometry.. Male Low Ref..100 0. Mix thoroughly. Prozone Check No Ref. Serum.

) 3 1 * (. Stable for 8 days at 2-8ºC.) (.. Abs... Female High 65 g/L 80 g/L 65 g/L 80 g/L Correlation Factor Correlation Offset 1.100 0. Abs. - Interferences: Hemoglobin (0. Male High Ref.100 3..2 g/L) and bilirubin (15 mg/dL) interfere.000 0.) No Protein No 25 mL 250 µ L 250 µ L 5 µL 3 µL 11.PROTEIN Spectrophotometric BIURET Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Protein in the sample reacts with copper (II) ion in alkaline medium forming a Reagent is ready to be used.... heparinized plasma.. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Incubation Time Protein Endpoint 546 nm g/L 1 0 g/L 150 g/L (. Moderate lipemia does not affect the results. Linearity: Up to 150 g/L. Prozone Check No Ref.000 -0. coloured complex that can be measured by spectrophotometry. Serum.) . Data entered by the operator * assigned value of standard Version 0012 . Female Low Ref.. Male Low Ref.5 min. Low Limit R. High Limit Factor Yes (. High Limit -0.180 Reagent Blank Cal.000 Low Absorbance High Absorbance R. Low Limit Cal.. Anticoagulants other than heparin should not be used.

forming a coloured complex that can be measured by spectrophotometry.00 g/L (.000 -0. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Incubation Time Protein-Urine Endpoint 620 nm g/L 2 0.000 Low Absorbance High Absorbance R.) No Protein-Urine No 25 mL 250 µ L 250 µ L 5 µL 3 µL 11. Abs.5 min..180 Reagent Blank Cal.00 g/L 4.14 g/L Correlation Factor Correlation Offset 1. cerebrospinal fluid.. Urine. Abs. Female High 0. Low Limit R.05 g/L 0. High Limit Factor Yes (.) (.000 0.05 g/L 0..14 g/L 0.. Male Low Ref.100 3.) 3 1 * (.. Stable for 8 days at 2-8ºC.) . Data entered by the operator * assigned value of standard Version 0012 .. High Limit -0.PROTEIN (URINE) Spectrophotometric PYROGALLOL RED Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Protein in the sample reacts with pyrogallol red and molybdate in acid medium Reagent is ready to be used. Female Low Ref.. Male High Ref... Low Limit Cal. - Linearity: Up to 4 g/L. Prozone Check No Ref..100 0.

000 -0.25 mmol/L) interfere.200 Reagent Blank Cal. a Reagent is ready to be used coloured complex that can be measured by spectrophotometry.) 3 1 * (. EDTA.3 mmol/L) and bilirubin (0. High Limit Factor Yes (. ascorbic acid (0. - Interferences: Hemoglobin (3 g/L). Lipemia does not affect results..000 0.5 min. Low Limit Cal. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Incubation Time Triglycerides Endpoint 505 nm mg/dL 0 0 mg/dL 600 mg/dL (..000 Low Absorbance High Absorbance R. Linearity: Up to 600 mg/dL.. Male High Ref. Prozone Check No Ref. High Limit -0..TRIGLYCERIDES Enzymatic-spectrophotometric GLYCEROL PHOSPHATE OXIDASE/PEROXIDASE Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Triglycerides in the sample originates..) (. Heparin. by means of some coupled reactions.) . Data entered by the operator * assigned value of standard Version 0012 . Abs. Serum or plasma. Abs.. Stable for 5 days at 2-8ºC. Female High 60 mg/dL 150 mg/dL 60 mg/dL 150 mg/dL Correlation Factor Correlation Offset 1.. oxalate and fluoride may be used as anticoagulants...100 3.100 0.) No Triglycerides No 25 mL 300 µ L 300 µ L 3 µL 2 µL 11. Male Low Ref.. Low Limit R. Female Low Ref.

Reagent 2: Use the Reagent B.) No Urea Color No 25 mL 220 µ L 220 µ L 2 µL 1 µL 25 mL 180 µ L 180 µ L 6. High Limit -0. Linearity: Up to 300 mg/dL. - Interferences: Ammonium salts of the anticoagulants interfere. Prozone Check No Ref. Mix thoroughly..) .000 0. Stable for 7 days at 2-8ºC. plasma. Heparin is recommended as anticoagulant.. Abs. Male High Ref..000 Low Absorbance High Absorbance R. Female Low Ref. urine.5 min.000 -0.200 Reagent Blank Cal.100 0.. bottle. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off DUAL MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume R2 Bottle Normal Volume Rerun Volume Incubation Time Urea Color Endpoint 620 nm mg/dL 0 0 mg/dL 300 mg/dL (. Low Limit Cal. a Reagent 1: Transfer the contents of one Reagent A2 vial into a Reagent A1 coloured complex that can be measured by spectrophotometry. Female High 10 mg/dL 50 mg/dL 10 mg/dL 50 mg/dL Correlation Factor Correlation Offset 1... High Limit Factor Yes (.100 3. Serum. Stable for 2 months at 2-8ºC..UREA/BUN Enzymatic-spectrophotometric COLOR Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Urine in the sample originates.. by means of some coupled reactions..) (. Male Low Ref. Data entered by the operator * assigned value of standard Version 0012 . Low Limit R.) 3 1 * (. Abs..

NADH Reagent 1: Use the Reagent A. Deviation Reagent Blank Cal.) .) No Urea No 25 mL 300 µ L 250 µ L 2 µL 2 µL 32. Female High 10 mg/dL 50 mg/dL 10 mg/dL 50 mg/dL Correlation Factor Correlation Offset 1.) Low Absorbance High Absorbance R. Urea No 25 mL 270 µ L 250 µ L 2 µL 2 µL 25 mL 30 µ L 30 µ L No No 24. Prozone Check No Ref.. by means of some coupled reactions.. Abs.000 0.) 3 1 * (.. Serum. Abs.000 -0. Time DUAL MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume R2 Bottle Normal Volume Rerun Volume Predilution Slope Blank Delay. that can be measured by spectrophotometry. urine...000 -0.100 3. High Limit R. 70 sec...100 2. Abs.100 3. 77 sec..UREA/BUN Enzymatic-spectrophotometric ULTRAVIOLET Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Urea in the sample consumes..100 Yes (. High Limit Factor -0. Low Limit R. Low Limit R. High Limit Factor -0. High Limit R... Male High Ref. Abs.000 0. Data entered by the operator * assigned value of standard Version 0012 . Abs. Time Urea Twopoint 340 nm mg/dL 0 0 U/L 300 U/L (. Reagent 2: Use the Reagent B. Male Low Ref. plasma. Female Low Ref.000 Low Absorbance High Absorbance R. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Delay. Deviation Reagent Blank Cal... min. min. Heparin is recommended as anticoagulant..100 2. Stable for 7 days at 2-8ºC. - Interferences: Ammonium salts of the anticoagulants interfere. Abs. Low Limit Cal. Linearity: Up to 300 mg/dL. Low Limit Cal.000 0.) (.) (.100 Yes (.

000 Low Absorbance High Absorbance R. Lipemia may affect the results Linearity: Up to 25 mg/dL. Abs..4 mg/dL 7. Prozone Check No Ref. by means of some coupled reactions.4 mg/dL 5. heparinized plasma.. Data entered by the operator * assigned value of standard Version 0012 . Female Low Ref.200 Reagent Blank Cal..) (.100 3. - Interferences: Hemoglobin (1 g/L).100 0. High Limit -0.3 mmol/L) and bilirubin (15 mg/dL) do not interfere. Male High Ref. Low Limit R. Magnesium in serum or plasma is stable for 10 days at 2-8ºC..) No Uric acid No 25 mL 250 µ L 250 µ L 6 µL 3 µL 11. Anticoagulants other than heparin should not be used. High Limit Factor Yes (.. ascorbic acid (0. Low Limit Cal.000 -0..) 3 1 * (. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Incubation Time Uric acid Endpoint 505 nm mg/dL 1 0 mg/dL 25 mg/dL (. Female High 3. Serum. a Reagent is ready to be used.) .URIC ACID Enzymatic-spectrophotometric URICASE/PEROXIDASE Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Uric acid in the sample originates. Male Low Ref.7 mg/dL Correlation Factor Correlation Offset 1..000 0. Abs..5 min...0 mg/dL 2. coloured complex that can be measured by spectrophotometry.

Male Low Ref. Low Limit R.. two ASO Twopoint 546 nm IU/mL 0 0 IU/mL 800 IU/mL (.900 0.. Mix with streptolysin O.. Prozone Check No Ref. ASO concentration and can be measured by turbidimetry... Male High Ref.050 0.000 -0. Abs. Abs. High Limit Factor -0.. 110 sec. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Point one...ANTI-STREPTOLYSIN O (ASO) Turbidimetry LATEX Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Anti-streptolysin O (ASO) causes agglutination of the latex particles coated Working Reagent: Pour the contents of a Latex vial into a Diluent bottle.050 3. Female High 0 IU/mL 200 IU/mL 0 IU/mL 200 IU/mL Correlation Factor Correlation Offset 1. Stable for 20 days at 2-8ºC.) 3 1 * (.000 Low Absorbance High Absorbance R.. Low Limit Cal. Abs.) No ASO No 25 mL 300 µ L 300 µ L 3 µL 2 µL 12. High Limit R. Stable for 7 days at 2-8 ºC. The agglutination of the latex particles is proportional to the thoroughly. - Linearity: Up to 800 IU/mL...) (. Hemolyzed or lipemic samples are not suitable for testing.150 Yes (. Deviation Reagent Blank Cal.000 0. Serum. Female Low Ref. Data entered by the operator * assigned value of standard Version 0012 .) .

COMPLEMENT COMPONENT C3 Turbidimetry Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Complement component C3 precipitates in the presence of anti-human C3 Reagent 1: Use the Reagent A.. two Complement C3 Twopoint 340 nm mg/dL 0 0 mg/dL 600 mg/dL (.. Low Limit R..) (. - The measurement interval depends on concentration of the highest standard. antibodies.000 0.000 Yes (. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off DUAL MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume R2 Bottle Normal Volume Rerun Volume Predilution Slope Blank Point one.. Female High 90 mg/dL 180 mg/dL 90 mg/dL 180 mg/dL Correlation Factor Correlation Offset 1. High Limit Factor -0. Hemolyzed or lipemic samples are not suitable for testing. falsely low values will be obtained when C3 is present in the sample at a concentration higher than 600 mg/dL.) No Complement C3 No 25 mL 220 µ L 220 µ L 3 µL 3 µL 5 mL 50 µ L 50 µ L No No -3. Abs...000 -0. Female Low Ref.. Stable 2 days at 2-8 ºC.050 3.. Male High Ref. Prozone Check No Ref. The originated turbidity is proportional to the C3 concentration and Reagent 2: Use the Reagent B. Data entered by the operator * assigned value of standard Version 0109 .) 2 5 * (.) 1.000 Low Absorbance High Absorbance R. Abs. can be measured by turbidimetry. Abs. Due to the zone effect. Deviation Reagent Blank Cal.. Male Low Ref.000 0. 236 sec. Serum or plasma treated with heparin or EDTA.00 . Low Limit Cal.. High Limit R.050 3.

... Hemolyzed or lipemic samples are not suitable for testing..000 0. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off DUAL MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume R2 Bottle Normal Volume Rerun Volume Predilution Slope Blank Point one. two Complement C4 Twopoint 340 nm mg/dL 0 0 mg/dL 150 mg/dL (.) No Complement C4 No 25 mL 220 µ L 220 µ L 9 µL 4 µL 5 mL 50 µ L 50 µ L No No -3.) 1. Data entered by the operator * assigned value of standard Version 0102 . Prozone Check No Ref.. Serum or plasma treated with heparin or EDTA. - The measurement interval depends on concentration of the highest standard...COMPLEMENT COMPONENT C4 Turbidimetry Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Complement component C4 precipitates in the presence of anti-human C4 Reagent 1: Use the Reagent A. can be measured by turbidimetry..00 . Male Low Ref.. Abs. Male High Ref. falsely low values will be obtained when C4 is present in the sample at a concentration higher than 150 mg/dL. 236 sec.000 Low Absorbance High Absorbance R.) (. Female High 10 mg/dL 40 mg/dL 10 mg/dL 40 mg/dL Correlation Factor Correlation Offset 1..000 0. High Limit Factor -0. Low Limit Cal. Abs.) 2 5 * (. Due to the zone effect. Deviation Reagent Blank Cal.000 -0. antibodies.050 3. Low Limit R. The originated turbidity is proportional to the C4 concentration and Reagent 2: Use the Reagent B.050 3.000 Yes (. High Limit R. Stable 2 days at 2-8 ºC. Female Low Ref. Abs.

. Serum...000 -0. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Point one. Male Low Ref..050 0. Female Low Ref. Mix coated with anti-human C-reactive protein. Hemolyzed or lipemic samples are not suitable for testing.000 Low Absorbance High Absorbance R.) 3 1 * (.000 Yes (. up to 200 IU/mL. Abs.C-REACTIVE PROTEIN (CRP) Turbidimetry LATEX Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Serum C-reactive protein (CRP) causes agglutination of the latex particles Working Reagent: Pour the contents of a Latex vial into a Diluent bottle. Interferences: Rheumatoid factors. turbidimetry. The agglutination of the latex thoroughly.. Abs. particles is proportional to the CRP concentration and can be measured by Stable for 20 days at 2-8ºC. Low Limit Cal. do not interfere. Stable for 7 days at 2-8 ºC.050 3.) (.000 0.. High Limit Factor -0. Female High 0 mg/L 6 mg/L 0 mg/L 6 mg/L Correlation Factor Correlation Offset 1. Low Limit R.900 2..) No CRP No 25 mL 398 µ L 398 µ L 2 µL 1 µL 12. two CRP Twopoint 546 nm mg/L 0 0 mg/L 150 mg/L (.. Deviation Reagent Blank Cal. Male High Ref. Data entered by the operator * assigned value of standard Version 0012 . Prozone Check No Ref. Abs. High Limit R...) . - Linearity: Up to 150 mg/L. 110 sec..

Male High Ref. Abs.) 3 1 * (. Deviation Reagent Blank Cal. Male Low Ref. turbidimetry.. Female High 0 mg/L 5 mg/L 0 mg/L 5 mg/L Correlation Factor Correlation Offset 1..) (. Bilirubin (>10 mg/dL) and rheumatoid factors (>75 IU/mL) may interfere..000 Low Absorbance High Absorbance R. Prozone Check No Ref.. Data entered by the operator * assigned value of standard Version 0203 .000 1. Mix thoroughly.. High Limit R. Low Limit R.000 Yes (. particles is proportional to the CRP concentration and can be measured by Stable for 20 days at 2-8ºC.050 3. Stable for 7 days at 2-8 ºC. Female Low Ref. 304 sec.000 0. two CRP-hs Twopoint 546 nm mg/L 1 0 mg/L 15 mg/L (.. Low Limit Cal. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Point one.) .06-15 mg/L.) No CRP-hs No 25 mL 225 µ L 225 µ L 3 µL 2 µL 12.700 2. Interferences: Hemoglobin (10 g/L) and lipemia (triglycerides 10 g/L) do not interfere.. Abs.. High Limit Factor -0.. Abs.. - Measurement: 0.000 1. The agglutination of the latex bottle. Serum.C-REACTIVE PROTEIN-hs (CRP-hs) Turbidimetry LATEX-HIGH SENSITIVITY Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Serum C-reactive protein (CRP) causes agglutination of the latex particles Working Reagent: Pour the contents of a Reagent B vial into a Reagent A coated with anti-human C-reactive protein.

. High Limit Factor -0. - The measurement interval depends on concentration of the highest standard.. Female Low Ref. Serum or plasma treated with heparin or EDTA. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off DUAL MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume R2 Bottle Normal Volume Rerun Volume Predilution Slope Blank Point one. Abs. Male Low Ref. concentration and can be measured by turbidimetry.050 3.000 0.000 0...000 Yes (. A antibodies. Low Limit Cal. Due to the zone effect... Prozone Check No Ref.050 3. two Ig A Twopoint 620 nm mg/dL 0 0 mg/dL 1300 mg/dL (. Deviation Reagent Blank Cal. falsely low values will be obtained when IgA is present in the sample at a concentration higher than 1300 mg/dL. Male High Ref.. Hemolyzed or lipemic samples are not suitable for testing.) 1. Abs.00 ... Data entered by the operator * assigned value of standard Version 0110 . Use the Working Reagent if MONO MODE.. Abs.IMMUNOGLOBULIN A (IgA) Turbidimetry Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Immunoglobulin A precipitates in the presence of anti-human immunoglobulin Reagent 1: Use the Reagent A. High Limit R. 236 sec. Stable for 7 days at 2-8 ºC.000 Low Absorbance High Absorbance R.) No Ig A No 25 mL 220 µ L 220 µ L 3 µL 2 µL 5 mL 50 µ L 50 µ L No No -3. The originated turbidity is proportional to the immunoglobulin A Reagent 2: Use the Reagent B.000 -0.) (. Low Limit R.) 2 5 * (. Female High 70 mg/dL 400 mg/dL 70 mg/dL 400 mg/dL Correlation Factor Correlation Offset 1.

000 -0.000 Low Absorbance High Absorbance R. two MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Point one. 236 sec. Male Low Ref.050 3. Female High 700 mg/dL 1600 mg/dL 700 mg/dL 1600 mg/dL Correlation Factor Correlation Offset 1.. two Ig G Twopoint 620 nm mg/dL 0 0 mg/dL 8000 mg/dL (.000 0. Use the Working Reagent if MONO MODE.000 0. - The measurement interval depends on concentration of the highest standard.) 1. Serum or plasma treated with heparin or EDTA. Abs. Hemolyzed or lipemic samples are not suitable for testing.IMMUNOGLOBULIN G (IgG) Turbidimetry Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Immunoglobulin G precipitates in the presence of anti-human immunoglobulin Reagent 1: Use the Reagent A. Deviation Reagent Blank Cal. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off DUAL MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume R2 Bottle Normal Volume Rerun Volume Predilution Slope Blank Point one. G antibodies.. Low Limit R. Prozone Check No Ref... Abs..) 2 5 * (. Abs.. Abs. Low Limit 0. Ig G No 25 mL 250 µ L 250 µ L 5 µL 5 µL 12.00 Low Absorbance High Absorbance R.. Low Limit R. concentration and can be measured by turbidimetry. Stable for 7 days at 2-8 ºC.000 3. High Limit R. Abs. Low Limit Cal. The originated turbidity is proportional to the immunoglobulin G Reagent 2: Use the Reagent B.000 3. Deviation Reagent Blank Cal..100 Yes (. Female Low Ref. High Limit R.) (.000 0.050 3.000 0.. Male High Ref. Data entered by the operator * assigned value of standard Version 0109 .000 Yes (. 70 sec. Abs.) No Ig G No 25 mL 220 µ L 220 µ L 2 µL 2 µL 5 mL 50 µ L 50 µ L No No -3.. falsely low values will be obtained when IgG is present in the sample at a concentration higher than 8000 mg/dL..) .. High Limit Factor -0. Due to the zone effect.

concentration and can be measured by turbidimetry. Serum or plasma treated with heparin or EDTA. High Limit Factor -0.. - The measurement interval depends on concentration of the highest standard. Data entered by the operator * assigned value of standard Version 0102 . Male High Ref.000 -0.000 0..) (..050 3..000 Low Absorbance High Absorbance R. M antibodies. falsely low values will be obtained when IgM is present in the sample at a concentration higher than 600 mg/dL. Abs. two Ig M Twopoint 340 nm mg/dL 0 0 mg/dL 600 mg/dL (.) No Ig M No 25 mL 220 µ L 220 µ L 5 µL 3 µL 5 mL 50 µ L 50 µ L No No -3. Low Limit R.) 1. Female Low Ref. Prozone Check No Ref.000 Yes (.. Low Limit Cal. The originated turbidity is proportional to the immunoglobulin M Reagent 2: Use the Reagent B. Stable for 7 days at 2-8 ºC.050 3. Abs.000 0. Deviation Reagent Blank Cal. Due to the zone effect.) 2 5 * (. 236 sec.. Hemolyzed or lipemic samples are not suitable for testing. Male Low Ref. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off DUAL MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume R2 Bottle Normal Volume Rerun Volume Predilution Slope Blank Point one. High Limit R....00 . Female High 40 mg/dL 230 mg/dL 40 mg/dL 230 mg/dL Correlation Factor Correlation Offset 1.IMMUNOGLOBULIN M (IgM) Turbidimetry Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Immunoglobulin M precipitates in the presence of anti-human immunoglobulin Reagent 1: Use the Reagent A.. Abs.

) .. Abs.050 0. 110 sec. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off MONO MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume Point one.) (.. Deviation Reagent Blank Cal..050 3. Abs.. Male High Ref. Male Low Ref. High Limit R. Female High 0 mg/L 15 mg/L 0 mg/L 15 mg/L Correlation Factor Correlation Offset 1..) 3 1 * (.) No Albumin (Urine) No 25 mL 300 µ L 300 µ L 2 µL 1 µL 12. Low Limit R..000 0. Stable for 7 days at 2-8 ºC. Abs. Urine should be centrifugated befor analysis.. Female Low Ref..ALBUMIN (URINE) Turbidimetry LATEX Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Albumin in the urine sample causes agglutination of the latex particles coated Working Reagent: Pour the contents of a Latex vial into a Diluent bottle.200 Yes (.000 Low Absorbance High Absorbance R. Urine. Stable for 8 hours at 2-8ºC.. Low Limit Cal..000 -0. The zone effect will cause to obtain falsely low values when albumin is present in the sample at a concentration higher than 1000 mg/L. - Linearity: Up to 130 mg/L.900 0. High Limit Factor -0. two Albumin (Urine) Twopoint 546 nm mg/L 0 0 mg/L 130 mg/L (. Data entered by the operator * assigned value of standard Version 0012 . Prozone Check No Ref. Mix with anti-human albumin. The agglutination of the particles is proportional to thoroughly. the albumin concentration and can be measured by turbidimetry.

000 Yes (. human gamma-globulin. Stable for 7 days at 2-8 ºC. Abs.. Female Low Ref. Prozone Check No Ref. two RF Twopoint 620 nm IU/mL 1 0 IU/mL 120 IU/mL (.000 2..050 3. High Limit Factor -0...000 Low Absorbance High Absorbance R. Data entered by the operator * assigned value of standard Version 0012 .000 -0. Deviation Reagent Blank Cal.) 2 1 * (.. Abs. Abs. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off DUAL MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume R2 Bottle Normal Volume Rerun Volume Predilution Slope Blank Point one..050 3. to the RF concentration and can be measured by turbidimetry. Low Limit Cal. 120 sec.. Serum.) (. Male High Ref...000 0.. Female High 0 IU/mL 20 IU/mL 0 IU/mL 20 IU/mL Correlation Factor Correlation Offset 1.RHEUMATOID FACTORS (RF) Turbidimetry LATEX Linear calibration Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Rheumatoif factors (RF) cause agglutination of the latex particles coated with Reagent 1: Use the Diluent. Hemolyzed or lipemic samples are not suitable for testing.. Low Limit R.) No RF No 25 mL 350 µ L 270 µ L 3 µL 2 µL 25 mL 40 µ L 30 µ L No No -3. This method has not zone effect up to 800 IU/mL. Male Low Ref. High Limit R. - Linearity: Up to 120 IU/mL.) . The agglutination of the latex particles is proportional Reagent 2: Use the Latex.

..) (....) No RF No 25 mL 350 µ L 270 µ L 3 µL 2 µL 25 mL 40 µ L 30 µ L No No -3.050 3. to the RF concentration and can be measured by turbidimetry.000 0.) 2 5 * (... Serum. Female Low Ref. 120 sec. - Linearity: Up to highest value of standard. Abs.. Abs.) . Data entered by the operator * assigned value of standard Version 0106 . two RF Twopoint 620 nm IU/mL 1 0 IU/mL .RHEUMATOID FACTORS (RF) Turbidimetry LATEX Non linear calibration Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Rheumatoif factors (RF) cause agglutination of the latex particles coated with Reagent 1: Use the Diluent. Abs. Male Low Ref. Female High 0 IU/mL 20 IU/mL 0 IU/mL 20 IU/mL Correlation Factor Correlation Offset 1. The agglutination of the latex particles is proportional Reagent 2: Use the Latex... Deviation Reagent Blank Cal. Hemolyzed or lipemic samples are not suitable for testing.. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off DUAL MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume R2 Bottle Normal Volume Rerun Volume Predilution Slope Blank Point one.050 3.. Male High Ref. IU/mL (.000 Yes (.. This method has not zone effect up to 800 IU/mL. Low Limit Cal.000 2. Prozone Check No Ref. High Limit Factor -0. Low Limit R. High Limit R.000 -0. human gamma-globulin. Stable for 7 days at 2-8 ºC.000 Low Absorbance High Absorbance R.

- Interferences: Hemoglobin (10 g/L). Male Low Ref. be measured by turbidimetry.) 1.000 Yes (.) 2 5 * (. Low Limit Cal. bilirubin (20 mg/dL) and Rheumatoid factors (300 UI/mL) do not interfere.) No Transferrin No 25 mL 220 µ L 220 µ L 2 µL 4 µL 5 mL 50 µ L 50 µ L No No -3. Stable for 7 days at 2-8ºC. Abs. High Limit Factor -0.050 3.... The originated turbidity is proportional to the transferrin concentration and can Reagent 2: Use the Reagent B.000 0.000 Low Absorbance High Absorbance R...00 ..050 3. Male High Ref... two Transferrin Twopoint 340 nm mg/dL 0 0 mg/dL . Abs. Serum or plasma treated with heparin or EDTA. High Limit R. 236 sec.. Data entered by the operator * assigned value of standard Version 0105 . Female Low Ref... Reagent 1: Use the Reagent A.TRANSFERRIN Turbidimetry Instrument: SELECTRA 2 Principle of the method Reagent preparation Samples Performance characteristics Transferrin precipitates in the presence of anti-human transferrin antibodies. Low Limit R.) (.000 -0. Deviation Reagent Blank Cal.. Abs. mg/dL (.000 0. Lipemia does not affect the results (5 g/L). Female High 200 mg/dL 360 mg/dL 200 mg/dL 360 mg/dL Correlation Factor Correlation Offset 1. Prozone Check No Ref. Instrument settings Name Mode Wavelength Units Decimals Low Concentration High Concentration Calibrator Name Repeat Number Concentration Interval Cut-off DUAL MODE Name Sample Blank R1 Bottle Normal Volume Rerun Volume Sample Normal Volume Rerun Volume R2 Bottle Normal Volume Rerun Volume Predilution Slope Blank Point one.