ALKALINE PHOSPHATASE (ALP

)
Continuous-spectrophotometric
DEA BUFFER

Instrument:

SELECTRA I

Principle of the method

Reagent preparation

Alkaline phosphatase (ALP) catalyzes in alkaline medium the transfer of the
phosphate group from 4-nitrophenylphosphate to diethanolamine (DEA),
liberating 4-nitrophenol. The catalytic concentration is determined from the rate
of 4-nitrophenol formation, measured at 405 nm.

Working Reagent: Dissolve the powder of a Reagent B vial with 20 mL of the
Reagent A bottle (if 10x20 mL size) or dissolve the contents of a Reagent B
vial with the entire volume of a Reagent A bottle (if 5x100 mL size).
Stable for 2 months at 2-8ºC.

Samples

Performance characteristics

Serum, plasma.
Alkaline phosphatase in serum or plasma is stable for 7 days at 2-8ºC.
Heparin may be used as anticoagulant

-

Linearity: up to 690 U/L.
Interferences: Fluoride, oxalate, citrate and EDTA as anticoagulants
interfere. Hemolysis interferes due to the alkaline phosphatase content in
red cells.

Instrument settings

Nombre
Nombre abrev.
Modo, Linealidad lim.
Retardo
Long. Onda
Unidades
Decimales
Vol. muestra
Vol. muest. rerun
Vol. reactivo
Vol. react. rerun
Absorb. infer.
Absorb. super.
Desv. Abs. react.
Lím. Infer. abs. react.
Lím. Super. abs. react.
Blanco reactivo
Blanco muestra

Fosfatasa alcalina
FAL
Cinética 10 %
51 seg.
405 nm
U/l
0
5
ul
2
ul
250 ul
253 ul
0.000 Abs.
3.000 Abs.
3.000 Abs.
0.000 Abs.
0.600 Abs.
Si
No

Calibrador
Repetir
Número
Concentración
Lím. Inferior
Lím. Superior
Factor
Ref. Hombre inf.
Ref. Hombre sup.
Ref. Mujer inf.
Ref. Mujer sup.
Ref. Ped. inf.
Ref. Ped sup.
Control 1
Control 2
Control 3
TEMPERATURA

2764.00
90 U/l
280 U/l
90
U/l
280 U/l
-

0

37 C

react. ped sup. Ref. Lím. 1. inf. 340 nm U/l 0 23 ul 5 ul 230 ul 248 ul 0. Si No Calibrador Repetir Número Concentración Lím. Mix gently. hombre inf. react. 3. hombre sup. Modo.0 2 U/l 41 U/l 2 U/l 41 U/l - 0 37 C .000 Abs. Alanine aminotransferase in serum is stable for 7 days at 2-8ºC. Ref.000 Abs. Stable for 2 months at 2-8ºC. by means of the lactate dehydrogenase (LDH) coupled reaction. react. muestra Vol. rerun Vol. Control 1 Control 2 Control 3 TEMPERATURA -1746. Lím. infer. Blanco reactivo Blanco muestra ALT/GPT ALT Cinética 10 % 70 seg. The bottle. Desv. measured at 340 nm. Ref. reducing the linearity of the method. Linearity: Up to 500 U/L. Linealidad lim. mujer inf. Retardo Long. reactivo Vol.ALANINE AMINOTRANSFERASE (ALT) Continuous-spectrophotometric IFCC Instrument: Principle of the method SELECTRA I Reagent preparation Alanine aminotransferase (ALT or GPT) catalyzes the transfer of the amino Working Reagent: Pour the contents of the Reagent B into the Reagent A group from alanine to 2-oxoglutarate.600 Abs. 3. super. super. abs. react. 3. infer. Absorb. catalytic concentration is determined from the rate of decrease of NADH. onda Unidades Decimales Vol. Ref. Abs. Samples Performance characteristics Serum. rerun Absorb.000 Abs. abs. - Interferences: High pyruvate in the sample will consume NADH during the delay time before measurements. forming pyruvate and glutamate. mujer sup. Instrument settings Nombre Nombre abrev. inferior Lím. superior Factor Ref. Ref. ped. muest.000 Abs.

urine. Hombre sup.500 Abs. 405 nm U/l 0 5 ul 2 ul 250 ul 253 ul 0. Retardo Long. Mujer inf. Ref. react. plasma. Interferences: Fluoride. rerun Vol. muest. inf. rerun Absorb. Mujer sup. measured at 405 nm. react. super. 3. citrate and EDTA as anticoagulants interfere. muestra Vol. Super. 3. oxalate. Modo. Absorb. Blanco reactivo Blanco muestra AMILASA AMY Cinética 10 % 32 seg. infer. Ref. Si No Calibrador Repetir Número Concentración Lím.100 Abs. Control 1 Control 2 Control 3 TEMPERATURA 3292. Ref. Abs. Ped. (CNP-G3) to 2-chloro-4-nitrophenol (CNP). Linealidad lim. Lím. Infer. react. plasma or urine is stable for 5 days at 2-8ºC. α-Amylase in serum. reactivo Vol. 0. Hombre inf. Superior Factor Ref. Ref.00 0 U/l 60 U/l 0 U/l 60 U/l - 0 37 C . Heparin may be used as anticoagulant - α-Amylase catalyzes the hydrolysis of 2-chloro-4-nitrophenyl-maltotrioside Reagent is ready to be used. Lím. -0.000 Abs.α-AMYLASE Continuous-spectrophotometric DIRECT SUBSTRATE Instrument: SELECTRA I Principle of the method Reagent preparation Samples Performance characteristics Serum. react. Ped sup. Linearity: up to 1300 U/L (serum) or 2600 U/L (urine).000 Abs. abs. The catalytic concentration is determined from the rate of 2-chloro-4-nitrophenol formation. Onda Unidades Decimales Vol. Ref. Inferior Lím. Instrument settings Nombre Nombre abrev. Desv. abs.000 Abs.

Lím.100 Abs. Samples Performance characteristics Serum. Ref. -0. Si NO Calibrador Repetir Número Concentración Lím. inferior Lím. react. Mix forming a coloured complex that can be measured by spectrophotometry. Hydroxyquinoline is included in the reagent to remove magnesium interference. Anticoagulants other than heparin should not be used. infer. super. 15. onda Unidades Decimales Vol. Ref. react. Lím. rerun Conc. heparinized plasma. reactivo Vol. mujer inf.CALCIUM Spectrophotometric METHYLTHYMOL BLUE Instrument: Principle of the method SELECTRA I Reagent preparation Calcium in the sample reacts with methylthymol blue in alkaline medium Working Reagent: Mix equal volumes of Reagent A and Reagent B. magnesium (10 mg/dL) and phosphate (20 mg/dL) do not interfere. **Se introduce automáticamente luego de la primer calibración CALCIO 3 1 * * * ** 9.000 mg/dl. inf. urine. super. Ref.5 g/L). ped sup.0 mg/dl 10. Retardo Long.5 min 620 nm mg/dl 1 3 ul 2 ul 300 ul 301 ul 4. Blanco reactivo Blanco muestra CALCIO CAL Punto final 11. rerun Vol. Control 1 Control 2 Control 3 TEMPERATURA *A introducir por el operador. superior Factor Ref. Stable for 2 days at 2-8ºC. Conc. muestra Vol.7 mg/dl - 0 37 C . bilirubin (20 mg/dL). Ref.7 mg/dl 9. Instrument settings Nombre Nombre abrev. Ref. mujer sup. - Linearity: Up to 15 mg/dL. infer. hombre inf.600 Abs.0 mg/dl 10. ped. muest. 0. Linealidad lim. abs. Modo.000 mg/dl. hombre sup. Calcium in serum or plasma is stable for 10 days at 2-8 ºC. thoroughly. abs. react. Interferences: Hemoglobin (1.

EDTA. oxalate and fluoride may be used as anticoagulants. Superior Factor Ref.100 Abs. Retardo Long. Linealidad lim. Ref. Super. Mujer inf.000 mg/dl. Mujer sup. - Interferences: Hemoglobin (3 g/L). Lipemia does not affect results. Hombre sup. 500. Lím. react. reactivo Vol. Heparin. Samples Performance characteristics Serum or plasma. react. Infer. Instrument settings Nombre Nombre abrev. react. Ref. Lím. Hombre inf. Conc.25 mmol/L) interfere. a coloured complex that can be measured by spectrophotometry.3 mmol/L) and bilirubin (0. Infer. abs. 0.5 min 505 nm mg/dl 0 3 ul 2 ul 250 ul 251 ul 0. muest.CHOLESTEROL Enzymatic-spectrophotometric CHOLESTEROL OXIDASE/PEROXIDASE Instrument: Principle of the method SELECTRA I Reagent preparation Free and esterified cholesterol in the sample originates. inf. Control 1 Control 2 Control 3 TEMPERATURA COLESTEROL 3 1 * * * ** 123 mg/dl 270 mg/dl 123 mg/dl 243 mg/dl - 0 37 C . Ped. Ped sup. by means of some Reagent is ready to be used coupled reactions. Blanco reactivo Blanco muestra COLESTEROL COL Punto final 11. rerun Vol. Inferior Lím. Ref. Stable for 7 days at 2-8ºC. muestra Vol. rerun Conc. Modo. Ref. Linearity: Up to 1000 mg/dL. ascorbic acid (0. Si NO Calibrador Repetir Número Concentración Lím.00 mg/dl. Super. Onda Unidades Decimales Vol. -0. abs. Ref.300 Abs.

Modo. measured at 340 nm. Si No Calibrador Repetir Número Concentración Lím. Linealidad lim. Lím. of the hexokinase (HK) and glucose-6-phosphate coupled reactions. by means Stable for 15 days at 2-8ºC. Blanco reactivo Blanco muestra CK-NAC CK Cinética 10 % 129 seg. - Linearity: up to 900 U/L. inferior Lím. muest. in the presence of Working Reagent: Empty the contents of a Reagent B vial into a Reagent A creatine phosphate. mujer sup. react. The catalytic concentration is bottle. onda Unidades Decimales Vol. super. ped. infer. ped sup. hombre sup. react. Ref.CREATINE KINASE (CK) Continuous-spectrophotometric IFCC Instrument: Principle of the method SELECTRA I Reagent preparation Creatine kinase (CK) catalyzes the phosphorylation of ADP.800 Abs.000 Abs. react. to form ATP and creatine. super.100 Abs. Absorb. Creatine kinase in serum is stable for 7 days at 2-8ºC. 3. Control 1 Control 2 Control 3 TEMPERATURA 4127. abs.000 Abs. Ref. react. Swirl gently. Lím. Ref. Samples Performance characteristics Serum. Ref.000 Abs. Abs. inf. mujer inf. hombre inf. Retardo Long. 0.00 24 U/l 195 U/l 24 U/l 170 U/l - 0 37 C . determined from the rate of NADPH formation. Desv. abs. 340 nm U/l 0 10 ul 2 ul 250 ul 258 ul 0. rerun Absorb. Ref. rerun Vol. -0. reactivo Vol. superior Factor Ref. 3. muestra Vol. infer. Instrument settings Nombre Nombre abrev.

react. 3. Linealidad lim. infer. abs. 0.100 Abs. super. 1er punto 2do punto Long. reactivo Vol. The complex formation rate is measured in a short period to thoroughly. Ref. Mix coloured complex. Linearity: Up to 20 mg/dL (serum or plasma). Desv. Abs. muest. Si NO Calibrador Repetir Número Concentración Lím. onda Unidades Decimales Vol. avoid interferences. 20. react.000 Abs -0. Ref. hombre inf. **Se introduce automáticamente luego de la primer calibración CREATININA 3 1 * * * ** 0.1 g/L). - Interferences: Hemoglobin (0. Lím. oxalate and fluoride may be used as anticoagulants. Ref. bilirubin (10 mg/dL).CREATININE Kinetic-spectrophotometric ALKALINE PICRATE Instrument: Principle of the method SELECTRA I Reagent preparation Creatinine in the sample reacts with picrate in alkaline medium forming a Working Reagent: Mix equal volumes of Reagent A and Reagent B.60 mg/dl 1. protein and ketonic bodies do not interfere. inferior Lím. Stable for 2 months at 2-8ºC. inf. Modo. Instrument settings Nombre Nombre abrev. mujer sup.10 mg/dl 0.500 Abs. Control 1 Control 2 Control 3 TEMPERATURA *A introducir por el operador. React Lím. Samples Performance characteristics Serum. Heparin. Creatinine in serum or plasma is stable for 24 hours at 2-8ºC. superior Factor Ref. ped.10 mg/dl - 0 37 C .300 mg/dl. Blanco reactivo Blanco muestra CREATININA CRE Dos puntos 32 seg 90 seg 505 nm mg/dl 2 25 ul 5 ul 250 ul 270 ul 0. plasma. muestra Vol. hombre sup. Conc.000 mg/dl. ped sup. rerun Vol.60 mg/dl 1. infer. super. Ref. mujer inf. EDTA. urine. Ref. abs. react. rerun Conc.

urine. abs. Interferences: Do not use hemolyzed sera. Conc.000 mg/dl. Infer. spectrophotometry. Lím.5 min 340 nm mg/dl 1 3 ul 2 ul 300 ul 301 ul 0.500 Abs.PHOSPHORUS Spectrophotometric PHOSPHOMOLYBDATE/UV Instrument: Principle of the method SELECTRA I Reagent preparation Inorganic phosphorus in the sample reacts with molybdate in acid medium Working Reagent: Mix 35 mL Reagent A + 15 mL Reagent B. Phosphorus in serum or plasma is stable for 7 days at 2-8ºC.5 mg/dl 2. Mix thoroughly. Samples Performance characteristics Serum.500 mg/dl. Mujer inf. inf.100 Abs. Hombre sup. Super. **Se introduce automáticamente luego de la primer calibración FOSFORO 3 1 * * * ** 2. Ref. rerun Vol. forming a phosphomolybdate complex that can be measured by Stable for 12 months at 15-30ºC. Superior Factor Ref. EDTA and fluoride may be used as anticoagulants. Modo. Super. Lím. Infer. react.7 mg/dl 4. Instrument settings Nombre Nombre abrev. Ped sup. Linealidad lim. Ref. react. Ref. Ref. Retardo Long. reactivo Vol. -0. muest. Mujer sup. Inferior Lím. rerun Conc.7 mg/dl 4. 0.5 mg/dl - 0 37 C . Linearity: Up to 20 mg/dL. Ref. plasma. react. Hombre inf. 20. muestra Vol. Blanco reactivo Blanco muestra FÓSFORO FOS Punto final 11. Ped. abs. Onda Unidades Decimales Vol. Si NO Calibrador Repetir Número Concentración Lím. Control 1 Control 2 Control 3 TEMPERATURA *A introducir por el operador.

Hemolysed samples are not suitable for testing. hombre sup. Conc. Ref. - Interferences: Hemoglobin (up to 100 mg/dL). 1er punto 2do punto Long. onda Unidades Decimales Vol. Control 1 Control 2 Control 3 TEMPERATURA *A introducir por el operador. super. inferior Lím. medium. abs. Linearity: Up to 7 mmol/L.000 µmol/l 7. hombre inf. react. Instrument settings Nombre Nombre abrev. ped sup. Ref. inf. infer. mujer sup. Ref. Stable for 1 week at 2-8ºC and for 2 months at –20ºC. rerun Conc. react.000) NO Calibrador Repetir Número Concentración Lím. Blanco reactivo Blanco muestra FRUCTOSAMINA FRUC Dos Puntos 285 seg 422 seg 546 nm µmol/l 0 30 ul 15 ul 250 ul 265 ul 0. muest. The rate of formazan at a given temperature is proportional to the serum glycated proteins Samples Performance characteristics Serum. Modo. reactivo Vol. React Lím.000 Abs -0. mujer inf. Lím. Abs. Ref. abs. react. Linealidad lim.000 µmol/l 3. super. bilirubin (up to 2 mg/dL). ped. superior Factor Ref. uric acid (up to 15 mg/dL) and lipidemia do not interfere with the test. muestra Vol. infer. **Se introduce automáticamente luego de la primer calibración FRUCT BIO 3 1 * * * ** 0 µmol/l 285 µmol/l 0 µmol/l 285 µmol/l 0 0 0 37 C . Desv.FRUCTOSAMINE Kinetic-spectrophotometric NBT Instrument: Principle of the method SELECTRA I Reagent preparation Serum glycated proteins reduce nitroblue tetrazolium (NBT) salts in alakaline Reagent is ready to be used.100 Abs 0. rerun Vol.800 Abs SI (-0. Ref.

Ref.000 Abs. - Gamma-glutamyltransferase (γ-GT) catalyzes the transfer of the γ-glutamyl Working Reagent: Reconstitute the contents of a Reagent B vial with 15 mL (if group from γ-glutamyl-3-carboxy-4-nitroanilide to glycylglycine. muest. carboxy-4-nitroaniline. ped. abs. rerun Vol.000 Abs. Lím. muestra Vol.10x15 mL size) or 50 mL (if 4x50 mL size) of Reagent A. Ref. hombre sup. Modo. 1. onda Unidades Decimales Vol. react. Control 1 Control 2 Control 3 TEMPERATURA 1111. Absorb. super. hombre inf. reactivo Vol.GAMMA-GLUTAMYLTRANSFERASE (γγ-GT) Continuous-spectrophotometric IFCC Instrument: SELECTRA I Principle of the method Reagent preparation Samples Performance characteristics Serum. Blanco reactivo Blanco muestra GGTP GGT Cinética 10 % 32 seg. 405 nm U/l 0 25 ul 5 ul 250 ul 270 ul 0. liberating 3. rerun Absorb. Linearity: up to 300 U/L. react. Ref. inf. mujer sup. mujer inf. Retardo Long. 3. The catalytic concentration is determined from the rate Stable for 2 months at 2-8ºC. Si No Calibrador Repetir Número Concentración Lím. react. Swirl gently. super. react.000 Abs. abs. infer. ped sup.100 Abs. superior Factor Ref. Lím. Gamma-glutamyltransferase in serum is stable for 5 days at 2-8ºC.000 Abs. Linealidad lim. -0. Ref. of 3-carboxy-4-nitroaniline formation. Abs. Ref. 3. inferior Lím. Instrument settings Nombre Nombre abrev. Desv.00 15 U/l 60 U/l 10 U/l 40 U/l - 0 37 C . infer.

react.300 Abs. Mujer sup. Onda Unidades Decimales Vol. Ref. Super. ascorbic acid (10 mg/dL) and bilirubin (15 mg/dL) interfere. oxalate and fluoride may be used as anticoagulants. Lím. muest. Ref. react. Moderate lipemia does not affect the results. Ped. - - Interferences: Hemoglobin (0. Conc.000 mg/dl. Ref. Blanco reactivo Blanco muestra 2 µL 300 µL 301 µL 10. Linealidad lim.000 mg/dl. Samples Performance characteristics Serum or plasma. Heparin. Control 1 Control 2 Control 3 TEMPERATURA 110 mg/dl 70 mg/dl 110 mg/dl *A introducir por el operador. react. rerun Conc. Infer. Instrument settings Nombre Nombre abrev. Lím. reactivo Vol. Ref. * * * * * * ** 70 mg/dl Ref. 0 37 C .100 Abs. abs. -0. Linearity: Up to 500 mg/dL. Hombre inf. 0. by means of some coupled reactions. **Se introduce automáticamente luego de la primer calibración. abs. inf. Stable for 7 days at 2-8ºC. Retardo Long. rerun Vol. Ped sup. Mujer inf. Infer.GLUCOSE Enzymatic-spectrophotometric GLUCOSE OXIDASE/PEROXIDASE Instrument: SELECTRA I Principle of the method Reagent preparation Glucose in the sample originates.3 g/L). EDTA. a Reagent is ready to be used coloured complex that can be measured by spectrophotometry. Modo. muestra GLUCOSA GLU Punto final 11. Hombre sup. 500. Si NO 3 µL Calibrador Repetir Número Concentración Lím. Inferior Lím. Super.5 min 505 nm mg/dl 0 Vol. Superior Factor Ref.

onda Unidades Decimales Vol. reactivo Vol. abs. Desv. ped.000 Abs. Ref. muest. Modo. inferior Lím. inf. hombre inf. Mix gently. infer. Linealidad lim. rerun Vol. mujer inf. 340 nm U/l 0 23 ul 5 ul 230 ul 248 ul 0. superior Factor Ref. Blanco reactivo Blanco muestra AST/GOT AST Cinética 10 % 70 seg. react. - Interferences: High pyruvate in the sample will consume NADH during the delay time before measurements. 3. Ref. infer. ped sup.000 Abs. Lím. Ref. Control 1 Control 2 Control 3 TEMPERATURA -1746. measured at 340 nm. super. Retardo Long.600 Abs. bottle. react. 1. hombre sup. Lím. react. react. Ref. Stable for 2 months at 2-8ºC. Linearity: Up to 500 U/L. Aspartate aminotransferase in serum is stable for 7 days at 2-8ºC. abs. forming oxalacetate and glutamate. mujer sup. 3. rerun Absorb.000 Abs. Abs. muestra Vol. reducing the linearity of the method. Absorb. Samples Performance characteristics Serum. 3. Ref.ASPARTATE AMINOTRANSFERASE (AST) Continuous-spectrophotometric IFCC Instrument: Principle of the method SELECTRA I Reagent preparation Aspartate aminotransferase (AST or GOT) catalyzes the transfer of the amino Working Reagent: Pour the contents of the Reagent B into the Reagent A group from aspartate to 2-oxoglutarate. Instrument settings Nombre Nombre abrev. Si No Calibrador Repetir Número Concentración Lím.0 2 U/l 42 U/l 2 U/l 42 U/l - 0 37 C . by means of the malate dehydrogenase (MDH) coupled reaction. The catalytic concentration is determined from the rate of decrease of NADH.000 Abs. super.

Absorb. - Interferences: Do not use hemolyzed sera. Stable for 7 days at 2-8ºC. inf. Ref. Ref. Instrument settings Nombre Nombre abrev.5 min 578 nm ug/dl 0 30 ul 15 ul 250 ul 265 ul 0. Blanco reactivo Blanco muestra HIERRO FE Punto final 11. infer. infer. Retardo Long.100 Abs. Lím. inferior Lím. -0. superior Factor Ref. Ref.IRON Spectrophotometric FERROZINE Instrument: Principle of the method SELECTRA I Reagent preparation Transferrin-bound ferric ions in the sample are released by guanidinium and Working Reagent: Transfer the contents of one Reagent B vial into a Reagent reduced to ferrous by means of hydroxylamine. reactivo Vol. react. HIERRO 3 1 * * * ** 70 U/l 155 U/l 55 U/l 140 U/l - 0 37 C . Samples Performance characteristics Serum or heparinized plasma. Mix thoroughly. Linearity: Up to 1000 µg/dL. **Se introduce automáticamente luego de la primer calibración. Modo. Ref. react. abs. muest. 0. hombre sup. super.300 Abs. ped. rerun Vol. Lím. mujer sup. Ref. mujer inf. super. react.000 ug/dl.000 ug/dl. Linealidad lim. hombre inf. Ferrous ions react with A bottle. 1. ferrozine forming a coloured complex that can be measured by Stable for 6 months at 2-8ºC. ped sup. Si SI Calibrador Repetir Número Concentración Lím. Control 1 Control 2 Control 3 TEMPERATURA *A introducir por el operador. onda Unidades Decimales Vol. rerun Conc. muestra Vol. abs. spectrophotometry.

Linearity: Up to 1500 U/L. The catalytic concentration is determined 20x3 mL size) or 15 mL (if 10x15 mL size)of Reagent A. Heparin may be used as anticoagulant. Absorb. react. 3. Control 1 Control 2 Control 3 0 TEMPERATURA 37 C -8095.LACTATE DEHYDROGENASE (LD/LDH) Continuous-spectrophotometric PYRUVATE Instrument: SELECTRA I Principle of the method Reagent preparation Lactate dehydrognase (LD or LDH) catalyzes the reduction of pyruvate by Working Reagent: Reconstitute the contents of a Reagent B vial with 3 mL (if NADH. hombre inf. 1.000 Abs. rerun Absorb. inf. Blanco reactivo Blanco muestra LDH LDH Cinética 10 % 32 seg 340 nm U/l 0 5 ul 2 ul 250 ul 253 ul 0. to form lactate and NAD+. hombre sup. Retardo Long. ped sup. superior Factor Ref. super. Linealidad lim. Abs. mujer sup. super. Si No Calibrador Repetir Número Concentración Lím. from the rate of decrease of NADH.00 207 U/l 414 U/l 207 U/l 414 U/l - 0 37 C to the high lactate . infer. - Interferences: Hemolysis interferes due dehydrogenase concentration in red cells. Samples Performance characteristics Serum or plasma. Ref. Ref.000 Abs.000 Abs. measured at 340 nm. react. mujer inf. Modo. ped. Swirl gently. Ref. onda Unidades Decimales Vol. abs. muestra Vol.400 Abs 3. Lím. 2. react. Instrument settings Nombre Nombre abrev. Stable for 2 months at 2-8ºC. Ref. react. Desv. muest. abs. inferior Lím. infer.000 Abs. Lactate dehydrogenase in serum or plasma is stable for 24 hours at 2-8ºC. Ref. rerun Vol. reactivo Vol. Lím.

EGTA is included in the reagent to remove calcium interference. Blanco reactivo Blanco muestra MAGNESIO MAG Punto final 11. coloured complex that can be measured by spectrophotometry. Anticoagulants other than heparin should not be used.MAGNESIUM Spectrophotometric CALMAGITE Instrument: SELECTRA I Principle of the method Reagent preparation Magnesium in the sample reacts with calmagite in alkaline medium forming a Reagent is ready to be used. infer. Ref. rerun Conc. mujer sup. abs. **Se introduce automáticamente luego de la primer calibración MAGNESIO 3 1 * * * ** 1. super.5 min 546 nm mg/dl 1 3 ul 2 ul 300 ul 301 ul 0. Ref. ped. 4. Modo. Instrument settings Nombre Nombre abrev. Ref. Si NO Calibrador Repetir Número Concentración Lím. -0.1 mg/dl - 0 37 C .100 Abs. super. muestra Vol. reactivo Vol. react.000 mg/dl.8 mg/dl 2. Retardo Long. Conc. Control 1 Control 2 Control 3 TEMPERATURA *A introducir por el operador. inferior Lím. Samples Performance characteristics Serum. Ref. Linealidad lim.900 Abs. Magnesium in serum or plasma is stable for 10 days at 2-8ºC.500 mg/dl.1 mg/dl 1.8 mg/dl 2. superior Factor Ref.5 g/L). muest. onda Unidades Decimales Vol. inf. Lím. react. mujer inf. calcium (20 mg/dL) and bilirubin (20 mg/dL) do not interfere. ped sup. hombre sup. infer. Linearity: Up to 4 mg/dL. - Interferences: Hemoglobin (1. rerun Vol. react. abs. Lím. 0. hombre inf. heparinized plasma. Ref.

Stable for 8 days at 2-8ºC. heparinized plasma. Ref.PROTEIN Spectrophotometric BIURET Instrument: Principle of the method SELECTRA I Reagent preparation Protein in the sample reacts with copper (II) ion in alkaline medium forming a Reagent is ready to be used. Ref.0 g/dl 8. super. react. Linearity: Up to 150 g/L. rerun Conc. Samples Performance characteristics Serum. react.000 g/dl -0.2 g/L) and bilirubin (15 mg/dL) interfere.5 min 546 nm g/dl 1 5 µL 3 µL 250 µL 252 µL 0.500 Abs SI NO Calibrador Repetir Número Concentración Lím.0 g/dl 8. coloured complex that can be measured by spectrophotometry. infer.0 g/dl 0 37 C . Ref. Blanco reactivo Blanco muestra PROTEINAS PRO Punto Final 11. Conc. Super. muestra Vol. abs. muest. inf.100 Abs 0.000 g/dl 10. Modo. reactivo Vol. hombre sup. ped sup.0 g/dl 6. Instrument settings Nombre Nombre abrev. inferior Lím. mujer inf. - Interferences: Hemoglobin (0. Linealidad lim. Moderate lipemia does not affect the results. abs. superior Factor Ref. Anticoagulants other than heparin should not be used. Rerun Vol. Retardo Long. Lím. **Se introduce automáticamente luego de la primer calibración * * * * * * ** 6. ped. Ref. onda Unidades Decimales Vol. Ref. react. hombre inf. Lím. Control 1 Control 2 Control 3 TEMPERATURA *A introducir por el operador. mujer sup. infer.

react. UREA 3 1 * * * ** 10 mg/dl 50 mg/dl 10 mg/dl 50 mg/dl - 0 37 C . NADH Working Reagent: Transfer the contents of one Reagent B vial into a Reagent that can be measured by spectrophotometry. Superior Factor Ref. Ref. by means of some coupled reactions.abs. Conc. Samples Performance characteristics Serum.00 mg/dl. hombre sup. Si NO Calibrador Repetir Número Concentración Lím. rerun Conc.000 mg/dl. Ref.000 Abs. react. Infer. Infer. Instrument settings Nombre Nombre abrev. A bottle. Lím. Ref. ped. Ref. Stable for 7 days at 2-8ºC. Muest. Onda Unidades Decimales Vol. inf.UREA/BUN Enzymatic-spectrophotometric ULTRAVIOLET Instrument: Principle of the method SELECTRA I Reagent preparation Urea in the sample consumes. Linealidad lim. 250. Stable for 2 months at 2-8ºC. plasma. Super. hombre inf. Lím. Linearity: Up to 300 mg/dL. urine. Modo. 1er punto 2o punto Long. 3. Blanco reactivo Blanco muestra UREA URE Dos puntos 51 seg 110 seg 340 nm mg/dl 0 3 ul 2 ul 399 ul 400 ul 5. Mix thoroughly. Desv. React. rerun Vol. Super. **Se introduce automáticamente luego de la primer calibración. ped sup. Muestra Vol. mujer sup. - Interferences: Ammonium salts of the anticoagulants interfere. Ref. 3. Heparin is recommended as anticoagulant. mujer inf.250 Abs. Reactivo Vol. abs. abs.000 Abs 1. Control 1 Control 2 Control 3 TEMPERATURA *A introducir por el operador.react. Inferior Lím.

Instrument settings Nombre Nombre abrev. Samples Performance characteristics Serum. hombre inf.7 mg/dl - 0 37 C .300 Abs. Linealidad lim. Lipemia may affect the results Linearity: Up to 25 mg/dL. Magnesium in serum or plasma is stable for 10 days at 2-8ºC.000 mg/dl. super. hombre sup. abs.3 mmol/L) and bilirubin (15 mg/dL) do not interfere. Ref. ped sup. muest. reactivo Vol.5 min 546 nm mg/dl 1 5 ul 3 ul 250 ul 252 ul 0.0 mg/dl 2. Conc. infer. heparinized plasma. mujer sup. Ref. 25. Ref. Modo. ascorbic acid (0. rerun Conc. -0.4 mg/dl 7.100 Abs. mujer inf.4 mg/dl 5. abs. 0. by means of some coupled reactions. superior Factor Ref. ped. a Reagent is ready to be used. Ref. coloured complex that can be measured by spectrophotometry. rerun Vol. inf. AC. Lím. react.URICO 3 1 * * * ** 3. Si NO Calibrador Repetir Número Concentración Lím.URIC ACID Enzymatic-spectrophotometric URICASE/PEROXIDASE Instrument: Principle of the method SELECTRA I Reagent preparation Uric acid in the sample originates. react. Retardo Long. Blanco reactivo Blanco muestra ACIDO URICO URI Punto final 11. Lím.000 mg/dl. Anticoagulants other than heparin should not be used. muestra Vol. **Se introduce automáticamente luego de la primer calibración. onda Unidades Decimales Vol. Control 1 Control 2 Control 3 TEMPERATURA *A introducir por el operador. - Interferences: Hemoglobin (1 g/L). infer. Ref. react. super. inferior Lím.