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  • Cryocell: 10 Micron Depth Description

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    Thiagarajan Kanagarajan
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    The Cryocell Sperm counting chamber is a simple to use device for rapid and accurate sperm count and motility evaluation directly from undiluted Semen. The lower main part has a glass base wherein the centre portion has been coated with a metal layer on which there is grid of 0.01 sq. Mm. The upper part is cover glass, which is to placed on the drop of the sample and firmly pressed on both the sides to remove the excessive sample.

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    The Cryocell Sperm counting chamber is a simple to use device for rapid and accurate sperm count and motility evaluation directly from undiluted Semen. The lower main part has a glass base wherein the centre portion has been coated with a metal layer on which there is grid of 0.01 sq. Mm. The upper part is cover glass, which is to placed on the drop of the sample and firmly pressed on both the sides to remove the excessive sample.

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    © All Rights Reserved
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    0 views2 pages

    Cryocell: 10 Micron Depth Description

    Uploaded by

    Thiagarajan Kanagarajan
    The Cryocell Sperm counting chamber is a simple to use device for rapid and accurate sperm count and motility evaluation directly from undiluted Semen. The lower main part has a glass base wherein the centre portion has been coated with a metal layer on which there is grid of 0.01 sq. Mm. The upper part is cover glass, which is to placed on the drop of the sample and firmly pressed on both the sides to remove the excessive sample.

    Copyright:

    © All Rights Reserved
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    of 2

    Cryocell

    TheCryocellSpermcountingchamberisasimpletousedeviceforrapidandaccurateSpermcount&motilityevaluation
    directlyfromundilutedSemen.

    10MICRONDEPTH
    DESCRIPTION
    TheChamberiscomposedoftwoparts:
    Thelowermainparthasaglassbasewhereinthecentreportionhasbeencoatedwithametallayeronwhichthereisgridof
    0.01sq.mm.Thisparthasbeenloweredby10micronsascomparedtotherestofthechamber.Itisonthisportionthatavery
    smalldropofthesampleisplaced.
    Figure:

    Theupperpartiscoverglass,whichistoplacedonthedropofthesample&firmlypressedonboththesidestoremovethe
    excessivesample,therebyensuringthatthespacebetweenthecoverslip&theGridisexactly10microns.

    PREPRATIONOFTHECHAMBER
    Beforeuse,makecertainthattheopposedglasssurfacesareabsolutelycleanandfreeofdust,sincethesizeoftheparticles
    ismorethan10microns.Youshouldbrushthesurfaceswithaverysoftbrush.(Providedwiththepack)
    Thisisahighprecisioninstrumentbuilttoverynarrowtolerances.Thusextracare&cleanlinessisadvised.Thedepthofthe
    chamberisguaranteedat10.0microns(+0.001microns).Anyspeckofdustdepositedoneachsideofthechamberorin
    chamberitselfcanrenderthisaccuracyvoid.Pleasethereforecarefullycleanallsurfacesofthechamber&thecoverslipwith
    waterandabrush&thereafterwithspecialLenspaper&adrybrush.

    SEMENANAYSIS
    Mixthespecimenwell,takingcaretoavoidformationofbubbles,withtheaidofarod,putasmalldropinthecentreofthe
    centrestrip.Graspthecoverglass&Placeitontheedges&pressfirmly.Thisensurestheautomaticspreadofthedropinto
    thethicknessof10microns.Itisrecommendedthatthedropshouldspreadontheentireareaofthecentreportion.Oncethe
    coverglassisinplaceavoidfurthertouching.Liftingandrecoveringasthismayaffecttheuniformspreadofthespermwithin
    thedrop.Liftthechamber&placeisonthestageofthemicroscopeusinga20xobjectiveanda10xeyepiece.Avoidusing
    the10xobjective,asthespermswithetoosmalltosee.

    You can use a 40x objective to do the morphological examination as well which was not possible with the conventional
    chambersduetothethicknessoftheircoverglasses.
    Afterfocusingmovethestageofthemicroscopeandlocatethegridinthecentreoftheviewarea.Unevendistributionofthe
    Spermsmeanthatthesamplewasnotmixedthoroughly.Ifspermscannotbeseeninonefocalplane,impropercleaningof
    theglasssurfacesissuspected.Ineithercaserepeatthisprocedureaftercorrectinghefault.

    MOTILITYESTIMATION
    Countallthenonmotilespermswithin(10continuoussquareseitherhorizontallyorvertically).Thencountthemotilesperms
    inthesamearea.Addingupboththenumberswouldgiveyouthetotalcountperml.(inMillions).Youcanthendividethe
    motilewiththetotalcounttoreachthepercentagemotility.Usingthesamemethodestimatethegradeofthemotilityfrom+1
    to+4.

    Thisestimationismuchmoreaccuratethanthatperformedfromtheordinaryslideswherethespermsmaybecompressedby
    thecoverslipandtheirmovementimpaired.

    Thecalculationthiswayismuchbetterthanonedoneonahemoctometerwhereoneendsuponlygettingthetotalcount&
    notthemotility.

    SPECIALCASES
    Bubbles:Ifbubblesarepresentinthegridarea,itisrecommendedthatthedropmaybereplacedbyanother,unlessthe
    bubblesaresmallenoughnottointerferewiththeanalysis.Largeparticlesofdust,threadsetc.willalsointerferewiththe
    countbychangingthedepthofthespace&insuchacasethedropshouldbereplaced.
    Majorvariationincountsbetweendropsofthesamespecimenmeans,thatthesamplewasnotmixeswelloristooviscousto
    mixcompletely.

    CLEANINGANDPREPRATIONFORREUSE
    Dipthebrushintocleanwaterandsimplywipebothsidesoftheglasses.Thensqueezethebrushandspongetheremaining
    wateroffthechamber.Finallydrythesurfaceswithspeciallenspaper.(Providedwiththepack)
    TheChamberisnowreadyforreuse.

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