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7/25/2011

GNROxidase Positive Fermenters

General Characteristics

Gram negative rods

Ferment glucose

GNROxidase Positive Fermenters

Oxidase positive

CLS418 Clinical MicrobiologyStudent Laboratory

Growth on BAP and MAC

Polar flagella (if motile)

Linsey Donner, MLS (ASCP)CM


School of Allied Health
Clinical Laboratory Science Program

GNROxidase Positive Fermenters

Vibrio cholera
Vibrio species

Vibrio cholera

Vibrio parahaemolyticus

Vibrio vulnificus

Colony Morphology

Thiosulfate citrate bile salts sucrose agar (TCBS)

pH 8.6

Yellow colony

Alkaline peptone broth

Vibrio cholera
Gram Stain

Straight or slightly curved GNR

pH 8.6

Grows on BAP and MAC

Vibrio cholera
Identification:

Lactose non-F

Sucrose F

ODC positive

LDC positive

Indole positive

Growth in 0% salt

Variable growth in 6% salt

7/25/2011

Vibrio cholera

Vibrio parahaemolyticus

Pathogenesis:
Cholera

Rice water stools

Colony Morphology:

Due to enterotoxin

Dehydration

Transmission

Treatment

Green colony

Halophilic

Man only host

TCBS agar

0.5% salt to 8% salt

http://healthmap.wordpress.com, 1850

Vibrio parahaemolyticus
Gram Stain
Straight or slightly curved GNR

Vibrio parahaemolyticus
Pathogenesis:
Food poisoning

Common in Japan, some in USA

Eat raw seafood or steamed clams

Vibrio parahaemolyticus
Identification:
Lactose non-F

Sucrose non-F

ODC positive

LDC positive

Indole positive

NO growth in 0% salt

Growth in 6% salt

Vibrio vulnificus
Colony Morphology:

TCBS agar

Colony is green

7/25/2011

Vibrio vulnificus
Gram Stain
Straight or slightly curved GNR

Vibrio vulnificus

Vibrio vulnificus
Identification:

Lactose variable

Sucrose variable

ODC variable

LDC positive

Indole positive

NO growth in 0% salt

Variable growth in 6% salt

Differentiation of Vibrio spp

Pathogenesis:

Intestinal

yellow

Septicemia can be fatal

Lactose

NF

NF

Increase incidence in liver disease patients

Sucrose

NF

ODC

Growth on
0% salt
Growth on
6% salt

Highly invasive

Wound infections

Aeromonas species
Colony morphology

Beta hemolytic on
sheep blood agar

green

V. vulnificus

Colony on
TCBS

V. cholera V. parahaemolyticus

green

Aeromonas species
Identification:

Lactose variable

Inositol non-F

Sucrose F

LDC, ADH, Indole,


Esculin & Gelatin positive

Gram stain

Straight GNR

ODC negative

Growth in 0% salt

No growth in 6% salt

7/25/2011

Aeromonas species
Pathogenesis:

Habitat

Di h
Diarrhea

UTI

Wound infections

Septicemia (liver/pancreatic cancer)

Osteomyelitis

Plesiomonas species
Identification:

Lactose & Sucrose non-F

Inositol F

LDC ADH,
LDC,
ADH ODC,
ODC & Indole positive

Esculin & Gelatin negative

Growth in 0% salt

No growth in 6% salt

Summary

Colony morphology

Water and sewage

Plesiomonas species

Non-hemolytic on sheep blood agar

Gram stain

Pleomorphic GNR

Plesiomonas species
Pathogenesis:

Habitat

Fresh water in tropical areas

Gastroenteritis

Who am I?

General characteristics
Growth characteristics & Identification
1. Vibrio species
2. Aeromonas species
3. Plesiomonas species

Clinical significance

Oxidase
Gram stain

TCBS

Oxidase positive, ODC +, LDC +, Indole +, Sucrose F

Vibrio cholera
Indole

7/25/2011

Oxidase

Pasteurella species

Who am I?

General Characteristics:
Short, straight GNRs

Blood agar plate

MacConkey agar plate

OF Dextrose

Ferments glucose

Oxidase positive

Growth on BAP

No growth on MAC

Non-motile

Oxidase +, Sucrose F, LDC +, ADH +, ODC -

Aeromonas species

Pasteurella multocida
Colony Morphology

Pasteurella multocida

BAP
1. Convex, smooth, gray

Gram Stain:

2 Nonhemolytic
2.

Sh t straight
Short,
t i ht GNR

3. Musty/mushroom odor

No growth on MAC

Pasteurella multocida
Identification:

Weak Glucose F (apple green)

Pasteurella multocida
Pathogenesis:

Izzy

Wound infection

Animal bites (cats, dogs)

Indole positive

ODC positive

Meningitis brain abscess


Meningitis,

Urease negative

Abdominal abscess

Penicillin (2 units): VERY S

Septicemia

Chronic pulmonary infections

Gracie

7/25/2011

Pasteurella multocida
Who am I?

Pathogenesis:
Wound infection
Animal bites (cats, dogs)
Zoey

Murphey

Gracie

Blood agar plate

MacConkey agar plate

OF Dextrose

Short GNR, Oxidase positive, Indole positive, Penicillin S

Pasteurella multocida

Key points quiz

Day 9

Studentlab

Organism

Day4quiz

Staphylococcus Facultative
aureus
anaerobe

GramStain
morphology
Gram+cocci in
s/pr/cl

Day5quiz

Streptococcus
pyogenes

Gram+cocci
s/pr/ch

Day5quiz

Day5quiz

Atmosphere

Facultative
anaerobe

Enterococcus
species

Facultative
anaerobe

Gram+cocci
s/pr/ch

Streptococcus
pneumoniae

Facultative
anaerobe

Gram+cocci
s/pr/ch
Lancetshape

Key points quiz

Day 9

AdditionalInfo.
Catalase +
Coagulase +
BetaHemolysis
Catalase =
PYR+
Bacitracin S
BetaHemolysis
Catalase =
PYR+
Gamma
Hemolysis
Catalase =
AlphaHemolysis
Optochin S

Studentlab

Organism

Atmosphere

GramStain
morphology

AdditionalInfo.

Day7quiz

Escherichiacoli

Facultative
anaerobe

Gram=bacilli

Oxidase =
LactoseF
Indole Pos

Day7quiz
y q

Neisseria
gonorrhoeae

Obligateaerobe
g
(Capnophilic)

Gram=diplococci
p

Oxidase +
Glucose+
MLS=

Day8quiz

Pseudomonas
aeruginosa

Obligateaerobe

Gram=bacilli

Oxidase +
GlucoseOxidizer
Greenpigment
Grapesmell

Day9quiz

Haemophilus
influenzae

Facultative
anaerobe
(Capnophilic)

Pleomorphic
Gram=coccobacilli

Oxidase +
RequiresXandV