Microbiology Chapter 1-2 Multiple Choice Quiz 1. Which of the following is a scientific name = Mycobacterium tuberculos is 2.

Which of the following is not a characteristic of bacteria = have the sa me shape 3. Which of the following is the most important element of Koch’s germ theo ry of disease? The animal shows disease symptoms when = microorganism is inocula ted into the animal 4. Recombinant DNA is = the DNA resulting when genes of two different organ isms are mixed 5. Which of the following statements is the best definition of biogenesis? = Living cells can only arise from preexisting cells 6. Which of the following is a beneficial activity of microorganisms? = all of the above 7. It has been said that bacteria are essential for the existence of life o n Earth. Which of the following would be the essential function performed by bac teria? = decomposed organic material and recycle elements 8. Which of the following is an example of bioremediation: = application of oil=degrading bacteria to an oil spill 9. Spallanzan’s conclusion about spontaneous generation was challenged beca use Lavoisier had just shown that oxygen was the vital component of air. Which o f the following statements is true? = some microbes do not require air. 10. Which of the following statements about E. coli is not true? E. coli was the first disease-causing bacterium identified by Koch 11. Assume E. coli are grown in a mutruent medium containing the radioisotop e 16N. After a 48 hour incubation period, the 16N would most likely be found in the E. coli’s = protein 12. If Pseudomonas bacteria are supplied with radioactively labeled cytosine , after a 24-hour incubation period this cytosine would most likely be found in the cell’s = DNA 13. If E. coli were grown in a medium containing the radioactive isotope 12P , the 32P would be found in all of the following molecules of the cell except = carbohydrates 14. A carbonated drink, ph3, is 10,000 time more acid than distilled water = 10,000 15. The best definition of ATP is that it is = a molecule that supplies ener gy to do work 16. Which of the following is an organic molecule = C₁₈H₂₉SO₃ (Styrofoam) 17. Classify wach of the molecules on the left as an acid, base or salt. Th e dissociation products of the molecules are shown to help you. HNO₃ → H+ +NO₃ = Acid H₂SO₄ → 2H+ + SO½ˉ = Acid NaOH → Na+ + OHˉ = Base MgSO₄ → Mg₂+ + SO½ = Salt

Chapter 3 and 4 Multiple Choice Quiz 1. Assume you stain Bacillus by applying malachite green with heat and then counter-stain with safranin. Through the microscope, the green structures are = impossible to identify 2. What is this question – answer is C 3. Carbolfuchsin can be used as a simple stain and a negative stain. As a s imple stain, the pH is = higher than the negative stain 4. Looking at the cell of a photosynthetic microorganism, you observe that the chloroplasts are green in brightfield microscopy and red in fluorescence mic roscopy. You conclude that =

Chlorophyll is fluorescent 5. Which of the following is not a functionally analogous pair of stains = nigrosin and malachite green 6. Which of the following pairs is mismatched = none of the above 7. Assume you stain Clostridium by applying a basic stain, carbolfuchsin, w ith hear, decolorizing with acid-alcohol, and counterstaining with an acidic sta in, nigrosin. Through the microscope, the endospores are 1---red, and the cells are stained 2---colorless 8. Assume that your are viewing a Gram-stained field of red cocci and blue bacilli through the microscope. You can safely conclude that you have = two diff erent species 9. In 1996, scientist described a new tapeworm parasite that had kicked at least one person. The initial examination of the patient’s abdominal mass was mo st likely made using = brightfield microscopy 10. Which of the following is not a modification of a compound light microsc ope = electron microscopy 11. Which of the following is not a distinguishing characteristic of prokary otic cells – they lack a plasma membrane 12. Which statement best describes what happens when a gram-positive bacteri um is placed in distilled water and penicillin - the cell will undergo osmotic lysis 13. Which statement best describes what happens when a gram-negative bacteri um is placed in distilled water and penicillin - water will move into the cell 14. Which statement best describes what happens when a gram-positive bacter ium is placed in an aqueous solution of lysozyme and 10% sucrose - no change wi ll result the solution is isotonic 15. Which of the following statements best describes what happens to a cell exposed to polymyxins that destroy phospholipids – intracellular contents will l eak from the cell 16. Which of the following is not true about fimbriae – they may be used for motility 17. Which of the following pairs are mismatched – pili ---- reproduction 18. Which of the following pairs is mismatched – ribosomes-----protein stora ge 19. You have isolated a motile, gram-positive cell with no visible nucleus. You can assume this cell has - ribosomes 20. The antibiotic amphothericin B disrupts plasma membranes by combining wi th sterols; it will affect all of the following cells except – bacterial cells Match the structures to their functions __D___ Cell wall a. attachment to surfaces __F___ endospores b. cell wall formation __A___ fimbriae c. motility ___C__ flagella d. protection from osmotic lysis _A_E__glycocalyx e. protection from phagocytes __I___ pili f. resting _B_H__ plasma membrane g. protein synthesis __G___ ribosomes h. selective permeability i. transfer of genetic material Match the following characteristics of eukaryotic cells with their function __C___ __D___ __G___ __A___ __F___ pericentriolar material chloroplasts Golgi complex lysosomes mitochondria a. b. c. d. e. digestive enzyme storage oxidation of fatty acids microtubule formation photosynthesis protein synthesis

__B___ peroxiisomes __E___ rough ER

f. respiration g. secretion

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