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YORK COLLEGE

OF
THE CITY UNIVERSITY OF NEW YORK
Chemistry 342 FHKV: Instrumental Analysis II
Dr. Ruel Z. B. Desamero
Jayson Vedad

Fall 2015

Class: Monday 12 12:50 PM (lect) and 2 6:50 PM (lab) in Room 3E11.


Office Hours: Tuesdays and Thursdays 1 2:30 PM in Room 3F01f.
Course Description
Instrumental techniques with an emphasis on spectroscopy: UV-visible, IR and atomic
absorption, NMR, fluorescence. Molecular modeling.
Course Objectives
At the completion of the course, the students should be able to:
1) Use a spreadsheet to help analyze and display quantitative data.
2) Operate the following spectroscopic instruments, UV-Vis Absorption, Fluorescence, Atomic
Absorption, FTIR and NMR.
3) Explain the principles that govern the operation of these spectroscopic instruments.
4) Utilize spectroscopic methods to identify unknown sample and to quantitate the components
of a mixture.
5) Estimate the IR spectrum of a given sample using computational methods.
6) Use spectroscopy to determine the pKa of some compounds.
7) Design an experiment to address a particular problem of interest.
Your Hours
Plan on spending about 2 hours preparing for each lab period, and 8 hours writing each report.
Text
No text will be required and my discussions can be found in the following recommended
books: (1) Introduction to Spectroscopy, 3rd ed., by Pavia, Lampman & Kriz (Brooks/Cole, 2001;
ISBN: 0030319617); (2) Quantitative Chemical Analysis, 7th ed., by Harris (Freeman, 2003, ISBN
0716770415); (3) Principles of Instrumental Analysis, 6th ed., by Skoog, Holler & Nieman
(Thompson, 2006, ISBN 9780495012016); (4) Vogels Textbook of Quantitative Chemical Analysis,
6th ed., Mendham, Denney. Barnes & Thomas, (Prentice Hall, 2000, ISBN 978-0-582-22628-9; and
(5) Undergraduate Instrumental Analysis, 6th ed., Robinson, Frame & Frame, (Marcel Decker, 2005,
ISBN 0-8247-5359-3
You will be assigned problems from A Guide to Microsoft Excel 2002 for Scientists and
Engineers, 3rd ed., by B.V. Liengme (Butterworth-Heinemann, 2002; ISBN: 070656131) so you must
have access to one.

Grading
Grades are based on your written lab reports, lab performance, a special project and midterm
final and practical examinations. Paper copies of your lab reports must be handed to me at the
beginning of the lab class two weeks after the day you performed the experiment. Moreover, soft
copies of the reports as well as all accompanying computer files must be uploaded using
"Blackboard" on the day of submission. Reports submitted late (after 2:10PM of the due date) will
be marked 50% less. Absolutely no lab report will be accepted more than two weeks late. A strict
policy on plagiarism will be imposed, student caught violating this policy is liable to be dismissed
from the class. Each student is required to submit 6 full reports (7 including the special project) on
the experiments done on the dates in bold (see Schedule). No make-up reports will be allowed.
There will be two exams given during the term, a midterm and a final. The midterm is scheduled
on Oct 14, practical exam on Nov 18 and the final exam is on the week of Dec 16. I will expect you
to have a calculator and to be able to answer questions pertaining to the experiments, from the
operation of the machine, the detection of the signal, calculations and the correlation signals to
associated molecular perturbation.
Grades will be based on the course work in the following proportions:
lab reports

30 %

mid term exam

15 %

lab performance/participation

5%

special project

15 %

practical exam

15 %

final exam

20%

Twenty (20) of the 100 points of the main lab reports will be allocated this way:
3 points
Format
6 points
Notebook/Data Files
5 points
Error propagation/units/ Significant Figures
6 points
Practical Tests
For instance, you will lose 1 point each time you report a measurement with incorrect significant
figures. But your losses on this are limited; you can't lose more than 5 points in each lab report to
significant figure errors.
The other 80 points will depend on things unique to each report. Answer every specific question
asked in the handout, give complete results including units, and show all the requested graphs. A
small part of your grade will depend on getting the correct result, but evaluating correctly your
own data is more important. You will also be required to submit electronic copies of the report and
all raw data (in binary and ASCII formats) as well as the files containing the processed data to
Blackboard.
Ten percent of your final grade will be based on how well you prepare for and perform your
experiments.

A group of 2 to 3 students are to find a topic approved by me for their special project. Each
group will have to research each topic and perform the necessary experiments. Project proposals
are due Oct 14. Once the topic is approved each group is to write an introduction and materials
and method section before the Spring Break. Towards the end of the semester (Nov 18) you will be
given two weeks to do your proposed experiments. A final write up is due on Dec 9. On Dec 9,
each group will give a 25 min power point presentation. Special projects are worth 15 % of your
final grade. Your score will be based on the originality and relevance of your project, the conduct
of the experiment, your power point presentation and your final report.
Your grade does not depend on how the other people in the class doyou are not competing
with them.
Laboratory notebooks
You will need one lab notebook with numbered pages, capable of making carbon copies. (You
can find one in our bookstore). The essential principles are:
1) Everything you do in the lab should be recorded directly into the notebook.
2) It should be possible for another person to repeat everything you have done without
discussing it with you.
3) Turn in the carbon copies from your lab notebook at the end of each lab period.
Your notebook must contain an outline of the experiments to be performed (preferably in
schematic form), the instrumental parameters used, and the unknown number. Filenames for all
datafiles must also be included. Feel free to note down all pertinent observations.
You will also need some storage devices for your computer work. If you are using flash or jump
drives, note that not all the computers in the lab can read them. Only those computers running at
least Windows 2000 can. DO NOT by all means move computers to access the USB ports in the
back. If you damage the instruments attached to the computers while doing this you will be
liable for its repair. Use the USB extensions provided.
You are expected to copy all of your data and spreadsheets onto floppies (or any other media),
and then remove them from the hard drives of the computers before you leave each day.
Reports
One of the goals of this course is to improve your skill at communicating scientific information.
Writing is still the main way of doing that, and that is the reason for written reports. Reports are to
be written on 8.5" 11" paper, not in the notebook. I strongly encourage you to use spreadsheets,
word processors, and other computer-based techniques. Also, when you are writing your reports
make it like you are writing a story about what you did in class. The only difference is that
everything you write must be substantiated by your data and proper references.
Laboratory Technique
You are expected to work safely and in an organized fashion, to treat delicate equipment with
care, and to clean up after yourself. You will be asked to clean equipment you have left dirty.
Remember you will be graded based on how well you prepare for and perform your experiments.
Laboratory Safety
You must wear eye protection, such as prescription glasses or safety glasses, at all times in the
laboratory. If you forget this rule, I will remind you, and if I forget, please remind me. Contact lenses

are allowed, but only with safety glasses. If you wear contacts, please let me know. Remember if you
are not sure about something ask!
Computers
If you have computer questions that you can't figure out from the computer help functions or
from manuals you can ask me or any other faculty members. Help us maintain the reliability of these
computers by not leaving anyfiles (downloaded files, your data, your report, etc) on the desktop or
any other folders.
Blackboard
Part of my lectures will be posted on Blackboard, make sure you have access to it. You must
check Blackboard regularly for announcements and handouts.
York College Policies
Policy on ABS/INC grades:
The following overview is condensed from York's grading policy website:
http://york.cuny.edu/academics/policies/grading-policies
"A student who, because of extenuating circumstances, is absent from the final examination
and has completed the work for the course with a passing average may be assigned an ABS grade.
The student, in consultation with the instructor, has up to three weeks in the subsequent semester
within which to take the final and have the grade resolved, even if not registered in the subsequent
semester.
A student who has taken the final examination, but, because of extenuating circumstances,
has not completed the work for the course, and has a passing average may, at the discretion of the
instructor, receive an INC grade. The student, in consultation with the instructor, has up to 10 weeks
in the subsequent semester to complete the work and have the grade resolved even if not registered
in the subsequent semester.
The grades of INC or ABS are not considered in computing the academic index. However, if
a grade change is not received by the Office of the Registrar within the above specified limits, the
grades of INC and ABS are changed to FIN and FAB, respectively. These grades are considered as F
grades when computing the academic index."
Policy on Academic Integrity:
Yorks Academic Integrity Policy & Procedures, developed to conform to the CUNY policy on
Academic Integrity, can be found at:
http://york.cuny.edu/president/legal-compliance/legal-affairs/cuny-legal-policiesprocedures/Academic-Integrity-Policy.pdf/view
Anyone caught cheating in an exam or plagiarizing reports will automatically earn a grade of F
for the course.
Policy on accommodations for disabled students:
Information about the services provided to students at York College can be found at the Office of
Services for Students with Disabilities, located in room AC-1G02, and on-line at:
http://york.cuny.edu/student-development/ossd

Tentative Course Schedule


Date

Lecture

Laboratory

Sept 2

Organization of course; lab


walkthrough; Errors

Discussion: General Spectroscopy;


UV-vis; and AAS. Exercise 1: Excel;
spreadsheet assignments.

Sept 9

Discussion: Fluorescence

UV-visible absorption spectroscopy


1: Beers law

Sept 30

Sept 16 Discussion: FTIR

Fluorescence of quinine

Sept 30

Sept 30 Discussion: FTIR

Atomic absorption spectroscopy

Oct 14

Oct 7

Discussion: Molecular Modelling IR spectroscopy 1: Identification of


materials

Oct 14

Discussion: experiments;
Project proposals due

Oct 21

Discussion: experiments; project UV-visible absorption spectroscopy


proposal
2: Analysis of a mixture

Oct 28

Discussion: experiments; project UV-visible absorption spectroscopy 3:


proposal
pKa determination

Nov 4

Discussion: experiments; project IR spectroscopy 2: Analysis of a


proposal
mixture

Nov 11

Discussion: experiments; project IR spectroscopy 3: pKa


proposal
determination

Nov 18

Discussion: Special Project

Practical Exam, Special Project

Nov 25

Discussion: Special Project

Special Project

Dec 2

Discussion: NMR Spectroscopy

NMR Spectroscopy; Check out

Dec 9

Discussion: Student presentation Student presentations

Dec 16

Final Exam (TBA)

Due
date

Oct 21

Mid term Exam. Exercise 2:


Molecular modeling techniques. Talk
about Project proposals.

*Experiments performed on dates in bold requires a full report.

Nov 4

Nov 18

Dec 9