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Stay healthy with DOST-developed guyabano supplements & tea

Aside from being rich in carbohydrates, dietary fiber, and Vitamins C, B1, and B2.
Studies by the Chemicals and Energy Division (CED) of the Department of Science and
Technologys Industrial Technology Development Institute (DOST-ITDI) show that
guyabano generally has high flavonoid content. Flavonoids are phytochemicals that have
been found to inhibit or even prevent the growth of viruses, carcinogens, and allergens.
These are just the few benefits that the DOST-ITDI aims to harness as it develops
and promotes guyabano as a natural dietary health supplement. Traditionally, guyabano
has been consumed by diabetics to lower their blood sugar, and tests showed that it even
outperforms Metformin, the most commonly used maintenance drug of diabetics, in
ITDIs CED, while adhering to WHO standards, processed and packaged guyabano
fruits and leaves into 250 and 300mg capsules or in two-gram teabags. Thus, guyabano
capsules and tea bags are now more convenient to use. They are standardized and
naturally processed, and thus guaranteed safe, says Annabelle Briones, CED chief
In addition, guyabano has been scientifically and traditionally proven to have great
natural benefits. It helps lower fever, spasms, heart rate, and blood pressure. It also helps
relieve pain, inflammation, and asthma. Consuming guyabano extract can also safely
prevent cancer cells from forming while effectively slowing down tumor growth. It also
helps stop the growth of harmful bacteria, viruses, fungi, and parasites, even as it
They also discovered that the green unripe guyabano fruit contains more flavonoids
than the yellowish ripe fruit. The leaves meanwhile are rich in tannins, fats and oils,
unsaturated steroids, and triterpenes, and again, more flavonoids.

Blood Cell Increasing Activity and Nutritional Value of

Formulated Suspension from Guyabano (Annona muricata L.,
Annonaceae) Crude Leaf Extract
Lowering of blood elements erythrocytes, leukocytes, and thrombocytes is a major
concern in the treatment of cancers. There is a need to discover natural agents that
improve blood condition. This study investigated the blood cell increasing activity and
nutritional value of a formulated suspension from Guyabano (Annona muricata L.,
Annonaceae) crude leaf extract. It also determined the extracts Approximate Effective
Dose (AED) and Median Effective Dose (ED50) as erythrocyte, leukocyte and
thrombocyte increasing agent by log dose interval.
Results show that Guyabano (A. muricata) crude leaf extract was non-toxic even at
dose level of 2000 mg/Kg body weight; hence, it can be considered safe, under Category
5 of OECD Guidelines. The AED of the Guyabano (A. muricata) crude leaf extract was
13.98 mg/kg body weight and 3.9815.84 mg/kg body weight on increasing

erythrocytes and leukocytes, respectively; while the extracts AED on increasing the
thrombocytes was indeterminate.
As for the Effective Dose on the 50% (ED50) of test rabbits, the Guyabano (A.
muricata) extract showed erythrocyte increasing activity at 16.93 mg/kg body weight;
leukocyte increasing activity ED50 at 10.60 mg/kg body weight; while thrombocyte
increasing activity ED50 at 18.03 mg/kg body weight. The formulated suspension of
Guyabano (A. muricata) crude leaf extract contains protein, sodium, iron, potassium,
calcium, magnesium, zinc and phosphorus.

Protective effects of Annona muricata linn. (Annonaceae)

leaf aqueous extract on serum lipid profiles and
oxidative stress in hepatocytes of streptozotocin-treated
diabetic rats

Extracts from various morphological parts of Annona muricata Linn. (Annonaceae)

are widely used medicinally in many parts of the world for the management, control
and/or treatment of a plethora of human ailments, including diabetes mellitus (DM). The
present study was undertaken to investigate the possible protective effects of A. muricata
leaf aqueous extract (AME) in rat experimental paradigms of DM. The animals used were
broadly divided into four (A, B, C and D) experimental groups.
Group A rats served as control animals and received distilled water in quantities
equivalent to the administered volumes of AME and reference drugs solutions
Diabetes mellitus was induced in Groups B and C rats by intraperitoneal injections of
streptozotocin (STZ, 70 mg kg-1).
Group C rats were additionally treated with AME (100 mg kg-1 day-1, p.o.) as from
day 3 post STZ injection, for four consecutive weeks.
Group D rats received AME (100 mg kg-1 day-1 p.o.) only for four weeks.
STZ treatment also decreased (p<0.05) CAT, GSH, SOD, GSH-Px activities, and
HDL levels. AME-treated Groups C and D rats showed significant decrease (p<0.05) in
elevated blood glucose, ROS, TBARS, TC, TG and LDL. Furthermore, AME treatment
significantly increased (p<0.05) antioxidant enzymes activities, as well as serum
insulin levels. The findings of this laboratory animal study suggest that A. muricata
extract has a protective, beneficial effect on hepatic tissues subjected to STZ-induced
oxidative stress, possibly by decreasing lipid peroxidation and indirectly enhancing
production of insulin and endogenous antioxidants.



of Annona

streptozotocininduced diabetic rats
The leaves of Annona muricata are used to manage diabetes and its complications.
The aim of this study was to evaluate the antidiabetic, antioxidant activities and the
potential toxicity of aqueous extract of Annona muricata in streptozotocin-induced
diabetic rats.
Oral administration of Annona muricata aqueous extract (100 mg/kg or 200 mg/kg)
was studied in normal and streptozotocin-induced diabetic rats. In long term treatment, 2
weeks after streptozotocin-induced diabetic rats, animals received plant extract during 28
consecutive days. For a protective effect, extract was administered 3 days prior to
streptozotocin exposure and animals were observed 2 weeks without treatment.
The plant extract was not effective in normal rats. In diabetic rats, single
administration of the extract significantly reduced blood glucose levels by 75% and
58.22% respectively at the dose of 100 mg/kg and 200 mg/kg as compared to the initial
value. Treatment of normal rats 3 days prior to diabetes induction showed that, Annona
muricata extract has no effect within 72 h following STZ injection. However, after 14
days post-treatment, the extract at the dose of 100 mg/kg significantly reduced blood
glucose levels as compared with initial value and diabetic control rats.
Immunohistochemical staining of pancreatic -cells of diabetic rats treated with the dose
of 100 mg/kg expressed strong staining for -cell compared to diabetic control. In a longterm study daily administration of Annona muricata aqueous extract for 28 days to
diabetic rats, reduced blood glucose levels, serum creatinine, MDA, AST, ALT activity,
and nitrite levels LDL-cholesterol. Total cholesterol, triglycerides, SOD, and CAT
activity contents were restored.

Possible mechanisms of action of

of Annona muricata (soursop) in
Annona muricata Linn (Annonaceae) (soursop) is a food plant reported to have
antihypertensive properties. The blood pressure reducing effect of its aqueous leaf extract
and the possible mechanisms that may be responsible was investigated.
Intravenous administration of an aqueous leaf extract (9.1748.5 mg/kg) of A.
muricata on the mean arterial pressure and heart rate were recorded invasively on

anaesthetized, normotensive SpragueDawley rats. Contractile responses of rat aortic

rings to the extract (0.54.0 mg/mL) were studied using standard organ bath techniques.
A. muricata (9.1748.5 mg/kg) caused significant (p < 0.05) dose-dependent
reduction in blood pressure without affecting the heart rates. The hypotensive effects
were unaffected by atropine (2 mg/kg), mepyramine (5 mg/kg), propranolol (1 mg/kg)
and L-NAME (5 mg/kg). A. muricata leaf aqueous extract significantly (p < 0.05) relaxed
phenylephrine (109104 M) and 80 mM KCl induced contractions in endothelium
intact and denuded aortic rings; and caused a significant (p < 0.05) rightward shift of the
Ca2+ dose response curves in Ca2+-free Krebs solution containing 0.1 mM EGTA.
The hypotensive effects of A. muricata are not mediated through muscarinic,
histaminergic, adrenergic and nitric oxide pathways, but through peripheral mechanisms
involving antagonism of Ca2+.
Evaluation of plants with presumed antihypertensive properties for
their potential to decrease peripheral resistance using isolated guinea pig
aortic rings precontracted with phenylephrine
All parts of the A. muricata tree are used in natural medicine in the tropics
including the bark, leaves, root and fruit-seeds. Generally the fruit and fruit juice
is taken for worms and parasites, to cool fevers, to increase mothers milk after
childbirth (lactagogue), and as an astringent for diarrhea and dysentery.
The crushed seeds are used as a vermifuge and antihelmintic against
internal and external parasites and worms. The bark, leaves and roots are
considered sedative, antispasmodic, antidiabetic, antihypertensive, smooth
muscle relaxant and nervine and a tea is made for various disorders for those
purposes. Many bioactive compounds (ellagic acid, tannins, flavonoids,
polyphenolic compounds) and phytochemicals have been found in A. muricata as
scientists have been studying its properties since the 1940s.
Its many uses in natural medicine have been validated by this scientific
research. The earliest studies were between 1941 and 1962. Several studies by
different researchers demonstrated that the leaf, bark, roots, stem and seed
extracts are antibacterial in vitro against numerous pathogens and that the bark
has antifungal properties.
Blood samples were collected from tail vein of rats after 10-12 h of overnight
fasting, transferred to sterilized centrifuge tubes and allowed for clotting at room
temperature. The blood samples were centrifuged for 10 min at 4,000 x g to
obtain serum. The serum were stored in freezer at 0c for later analysis of total
cholesterol (TC) and triglyceride (TG) high and low-density lipoproteins (HDL and
LDL)-cholesterol, very low density lipoprotein (VLDL) where all concentrations
are given in mmol/L