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01-Dec-15

Disease Identification

Food Microbiology

Salmonella Gastroenteritis / Salmonellosis


Salmonella typhi and S. paratyphi

TUTORIAL 3
NAME: CHIA FOOK SOON F13A061
HUSNA SYAZANA BT MD AMIR F13A108
FARID JIHADI B SULAIMAN F13A083
HAZIRAH BT MOHAMED NASIR F13A102
AHMAD HAFIZUDDIN B MOHD IDRUS F13A010

rod-shaped, Gram negative, non-sporulating,

facultative anaerobic motile bacterium.


mesophiles
natural inhabitants of gastrointestinal tracts of

animals, birds, pets, frogs, turtle and insects.

Disease and Symptoms

Food associated

Septicemia (blood poisoning)

typhoid or typhoid-like fever


Other milder symptoms include :

nausea, vomiting, abdominal cramps, diarrhea, chills, fever,


headache and prostration.

Sampling

raw meats
poultry
eggs
milk and dairy products
fish
shrimp
frog legs
yeast
coconut
sauces and salad dressing
cake mixes
cream-filled desserts and toppings
dried gelatin
peanut butter
cocoa
chocolate

Identification/ Analysis

Samples should not originate from live being that

Traditional/Conventional method

recently treated with antimicrobial drugs.


Swabs or aseptically collected from infected tissues,
intestinal and cloacal contents.
Nature and quantity of sample depend on whether is
taken from live being or carcasses.

Identification based on colony appearance on chromogenic

and other selective of agar media using classical


biochemical and serological testing.
Key biochemical

Serological testing

Fermentation of glucose

Use polyvalent antiserafor flagellar (H)


and somatic (O) antigens

Negative urease reaction


Lysine decorboxylase
Negative indole test
H2S production
Fermentation of dulcitol

01-Dec-15

Analysis cont.
Growth of Salmonella on Blood agar

Rapid method

Growth of Salmonella on Chromogenic

Rapid immunological identification and

Salmonella Esterase Agar


Green colonies of Salmonella on
Hektoen Enteric (HE) agar

forformation test based on latex agglutination


enzyme immunoassay(EIA)
Enzymelinked immunosorbent assay(ELISA)
Molecular method for identification of salmonella
enterica
-notability DNA hydrodisation
- Polymerase Chain Reaction (PCR) assay

Advantages
Traditional/Conventional method

Rapid method ; EIA

-well-known and conversed by all.

-fast

-simple technique.

-highly specific and sensitive

-identification of specific microbial


group is possible by the use of
appropriate selective and differential
medium.
-inexpensive method.

Disadvantages
Traditional/Conventional method

Rapid method ; EIA

-cumbersome, material intensive and


time consuming

-equipment/material for assay not


easily obtained.

-easy to perform and caliberate

-the requirement for large number of


glass wares and lack of precision.

-variations requires at least two binding


sites.

-scalable

-does not indicate the type and source


-all positives must have a confirmatory
of organism and variations in the degree test performed.
of bacterial metabolism.