3)An enzyme that makes a double stranded DNA copy from a single stranded

RNA template molecule is known as:
A. DNA polymerase
B. RNA polymerase
C. Reverse transcriptase
D. Phosphokinase
Correct answer : C. Reverse transcriptase

4)A segment of a eukaryotic gene that is not represented in the mature
messenger RNA is known as:
A. Intron
B. Exon
C. Plasmid
D. TATA box
Correct answer : A. Intron
5)Shine Dalgarno sequence in bacterial mRNA is near:
A. AUG codon
B. UAA codon
C. UAG codon
D. UGA codon
Correct answer : A. AUG codon
12)During replication of DNA, which one of the following enzymes
polymerizes the Okazaki fragments?
A. DNA Polymerase I
B. DNA Polymerase II
C. DNA Polymerase III
D. RNA Polymerase I
Correct answer : C. DNA Polymerase III

Specifically recognizes the promoter site 63)DNA rich in A-T pairs have (A) 1 Hydrogen bond (B) 2 Hydrogen bonds (C) 3 Hydrogen bonds(D) 4 Hydrogen bonds Ans-2 64). Binds the antibiotic Rifampicin B. tRNA C. rRNA D. 17% cytosine and 33% thymine. Specifically recognizes the promoter site D. c) Double—stranded RNA d) Double stranded DNA Correct answer : d) Double stranded DNA 21). The nucleic acid must be: a) Single-stranded RNA b) Single-stranded DNA. In DNA molecule (A) Guanine content does not equal cytosine content (B) Adenine content does not equal thymine content . 18% guanine. Is inhibited by -amanitin C. tRNA 14)A nucleic acid was analyzed and found to contain 32% adenine. snRNA Correct answer : B. Is part of the core enzyme Answer C. The sigma subunit of prokaryotic RNA Polymerase: A.13)Which type of RNA has the highest percentage of modified base? A. mRNA B.

(C) Adenine content equals uracil content (D) Guanine content equals cytosine content Ans-d 65)In humans end product of purine catabolism is (A) Uric acid (B) Urea (C) Allantoin (D) Xanthine Ans-a 66)Gout is a metabolic disorder of catabolism of (A) Pyrimidine (B) Purine (C) Alanine (D) Phenylalanine Ans-b 67)The smallest unit of DNA capable of coding for the synthesis of a polypeptide is (A) Operon (B) Repressor gene (C) Cistron (D) Replicon ans-c 68)The enzyme DNA ligase (A) Introduces superhelical twists (B) Connects the end of two DNA chains (C) Unwinds the double helix (D) Synthesises RNA primers Ans-b 69)Okazaki fragment is related to (A) DNA synthesis (B) Protein synthesis .

Plasmid D. AUG codon B. Intron B. RNA polymerase C. Reverse transcriptase D. Reverse transcriptase 14)A segment of a eukaryotic gene that is not represented in the mature messenger RNA is known as: A. Intron 15)Shine Dalgarno sequence in bacterial mRNA is near: A. DNA polymerase B.(C) mRNA formation (D) tRNA formation Ans-a 70)Direction of DNA synthesis is (A) 5’ -3’ (B) 3’ -5’ (C) Both (A) and (B) (D) None of these Ans-a 71)DNA fragments are sealed by (A) DNA polymerase II (B) DNA ligase (C) DNA gyrase (D) DNA topoisomerase II Ans-b 13)An enzyme that makes a double stranded DNA copy from a single stranded RNA template molecule is known as: A. Exon C. UAA codon . Phosphokinase Correct answer : C. TATA box Correct answer : A.

C. Transformation The correct answer is choice B. Transcription D. it requires DNA polymerase b. tRNA 27) The process involved in formation of protein from RNA is A. rRNA D. snRNA Correct answer : B. cytarabine inhibits DNA synthesis ans –b 68 . UAG codon D. mRNA B. Translation C.Which statement best describes Xanthine ? a) It is a direct precursor of Guanine b) It covalently binds to Allopurinol c) It is oxidized to form Uric acid d) It is oxidized to form Hypoxanthine . AUG codon 23)Which type of RNA has the highest percentage of modified base? A. tRNA C. UGA codon Correct answer : A. 41) The following are false about DNA synthesis: a. moves in a 5'---> 3' direction d. reverse transcriptase enzymes are involved c. Replication B.

methyl thymidine b) Ara –C c) Ribose phosphate d) PRPP Ans-d 70). The patient's condition is caused by an enzyme deficiency in which of the following biochemical pathways? A. . Ganglioside metabolism B. Monosaccharide C. Pyrimidine metabolism The correct answer is C. Purine metabolism D.Which of the following is a required substrate for purine biosynthesis a) 5.Which of the following is an analogue of hypoxanthine ? a) Ara C b) Allopurinol c) Ribose phosphate d) PRPP Ans-b 15 Which of the following molecules does not form part of DNA? (a) purine (b) pyrimidine (c) deoxyribose (d) amino acid Ans-d 17) A patient has Lesch-Nyhan syndrome caused by an absence of hypoxanthine-guanine phosphoribosyl transferase.Ans-c 69).

In DNA molecule (A) Guanine content does not equal cytosine content (B) Adenine content does not equal thymine content (C) Adenine content equals uracil content (D) Guanine content equals cytosine content Ans-d 53)In humans end product of purine catabolism is (A) Uric acid (B) Urea (C) Allantoin (D) Xanthine Ans-a 54)Gout is a metabolic disorder of catabolism of (A) Pyrimidine (B) Purine (C) Alanine (D) Phenylalanine Ans-b 55)The smallest unit of DNA capable of coding for the synthesis of a polypeptide is (A) Operon (B) Repressor gene (C) Cistron (D) Replicon ans-c .15: The four nitrogenous bases which form the code words for DNA language are (A) ACTU (B) UTAC (C) AGTU (D) AGCT Ans d 51)DNA rich in A-T pairs have (A) 1 Hydrogen bond (B) 2 Hydrogen bonds (C) 3 Hydrogen bonds(D) 4 Hydrogen bonds Ans-2 52).

The nucleic acid must be: a) Single-stranded RNA b) Single-stranded DNA. is free to rotate over about 180° Answer: Option A .ion C. 17% cytosine and 33% thymine. connect the sugar to the base B. stabilize Watson-Crick H-bonds D. 18% guanine. c) Double—stranded RNA d) Double stranded DNA Correct answer : d) Double stranded DNA 53) The glycosidic bonds in DNA and RNA A. can be hydrolyzed by OH.56)The enzyme DNA ligase (A) Introduces superhelical twists (B) Connects the end of two DNA chains (C) Unwinds the double helix (D) Synthesises RNA primers Ans-b 57)Okazaki fragment is related to (A) DNA synthesis (B) Protein synthesis (C) mRNA formation (D) tRNA formation Ans-a 58)Direction of DNA synthesis is (A) 5’ -3’ (B) 3’ -5’ (C) Both (A) and (B) (D) None of these Ans-a 59)DNA fragments are sealed by (A) DNA polymerase II (B) DNA ligase (C) DNA gyrase (D) DNA topoisomerase II Ans-b 60)A nucleic acid was analyzed and found to contain 32% adenine.

Acetyl-COA carboxylase Ans: (c) 55)The smallest unit of DNA capable of coding for the synthesis of a polypeptide is (A) Operon (B) Repressor gene (C) Cistron (D) Replicon ans-c 56) The most abundant free nucleotide in mammalian cells is a. Glutamine c. Propionyl-COA carboxylase b. ATP b.54) Biotin is not a coenzyme in . FAD Ans: (a) 58) An increased melting temperature of duplex DNA results from a high content of a. NAD c. Pyruvate carboxylase c. Adenine + Guanine b. Threonine Ans: (a) .(A1 89) a. Cytosine + Adenine Ans: (c) 59)DNA fragments are sealed by (A) DNA polymerase II (B) DNA ligase (C) DNA gyrase (D) DNA topoisomerase II Ans-b 60) In purine biosynthesis carbon atoms at 4 and 5 position and N at 7 positions are contributed by a. GTP d. Glycine b. Thymine + Cytosine c. Alanine d. Cytosine + Guanine d. Pyruvate dehydrogenase d.

a phosphate and a nitrogenous base contain deoxyribose. d. b. a phosphate and a nitrogenous base AnS-c 56) End product pf pyrimidine metabolism is a. DNA is also present in a. b. d. They They They They contain ribose. phosphate and nitrogen base Phosphate and nitrogen base Pentose sugar and phosphate Pentose sugar and nitrogen base Ans a 60) Chargaff’s rule state that . c. c. Uric acid Hypoxanthine Beta-Alanine None of the above ANs-C 57) Besides nucleus. b. c. d. c. c. d. b. b. d. a phosphate and a nitrogenous base are double-stranded and anti-parallel contain a pentose. Pentose sugar.55) Which of the following is true for ALL nucleotides? a. Uric acid Creatinine Xanthine Phoshphates anS-a 59) A nucleoside is formed of a. Mitochondria Lysosomes Golgi apparatus Ribosomes Ans a 58) Final product of purine metabolism is: a.

18% guanine. c. c. DNA ligase DNA Polymerase I DNA Polymerase III Primase ANs-c ANsd 62) The following are false about DNA synthesis: a. which of the following enzymes produces a short strand of RNA complementary to the template DNA with a free 3'-OH end? a. 17% cytosine and 33% thymine. Single-stranded RNA Single-stranded DNA. Double—stranded RNA Double stranded DNA Correct answer : d) Double stranded DNA 64) The replication of mRNA from the DNA of the gene is known as a. d. d. c. b. d.a. c. b. It requires DNA polymerase Reverse transcriptase enzymes are involved Moves in a 5'---> 3' direction Cytarabine inhibits DNA synthesis ans –b 63) A nucleic acid was analyzed and found to contain 32% adenine. d. b. d. b. b. Transduction Translation Transformation Transcription ANs-D 65) The TATA box . The nucleic acid must be: a. A+G=T+C A/T=G/C A=U=T=G A+T=G+C AnS-a 61) During DNA replication. c.

C 66) An enzyme that makes a double stranded DNA copy from a single stranded RNA template molecule is known as: a. c. Modification of bases Splicing of introns Trimming of 3’ end to CCA sequence Attachment of poly A tail Ans-C 68 )The sigma subunit of prokaryotic RNA Polymerase: a. d. d. DNA polymerase RNA polymerase Reverse transcriptase Phosphokinase Correct answer : C. except a.a. a. b. Present on the template strand Present about 70 bp away from transcription start site Serves as the signal for attachment of RNAP-II Act as a silencer of the gene ANs. b. b. d. c. d. Reverse transcriptase 67) All are post-transcriptional changes. b. c. Ans a Messenger RNA Ribosomal RNA Transfer RNA All of the above . Binds the antibiotic Rifampicin Is inhibited by amanitin Specifically recognizes the promoter site Is part of the core enzyme Answer C. Specifically recognizes the promoter site 69) The form of RNA that delivers information from DNA to be used in making a protein is _______________. b. c. d. c.

d. The synthesis of clotting factors Post translational modifications Antioxidant mechanisms The microsomal hydroxylation reactions Correct answer : B. b.70) Both Vitamin K and C are involved in: a. d.except: . c. c. Post translational modifications 71) Post translational modifications include all the following except: a. Glycosylation Hydroxylation Decarboxylation Phosphorylation ANs-C 72) Genes are a b c d Ribonucleic acid Deoxyribonucleic acid Lipoproteins Chromo proteins ANs-B 73) Euchromatin is in the region of DNA that is relatively a b c d Uncondensed Condensed Over condensed Partially condensed Ans-A 74) Function of endonucleases a b c d Cut DNA AT Specific DNA sequence To point out he coding regions Enhancers To find out antibiotic resistance ANs-A 75) Application of recombinant DNA technology include all the following . b.

a b c d Detection of oncogenes Synthesis of human proteins Inhibition of replication Gene Therapy ANs-C .