National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

Chairman
Amity University, Lucknow Campus
Chancellor
Amity University Rajasthan
Amity University Haryana
Dr. Aseem Chauhan

President
Amity University Mumbai

It gives me immense pleasure and sense of great pride that Amity Institute of Pharmacy,
Amity University, Uttar Pradesh, Lucknow Campus, is organizing a National Conference on
“Drug Discovery & Formulation Development” on February 23, 2016.
The National Conference is a step towards discussion and deliberation in sharing
knowledge on innovation and emerging areas in Drug Discovery and related fields. I am sure that
the presence of scientists and researchers from various leading centers of Pharmaceutical Science
in India will lead to a wonderful opportunity to exchange thoughts and ideas on contemporary
issues and will set new goals and targets for young researchers and scientists in the area of Drug
Discovery.
As an obligation towards our society, we must also contemplate how this research can be
applied and commercialized so that India can not only become a front runner in the field of New
Drug Discovery and Formulation Development but also become self-sufficient in the area of
health and medicine.
I wish organizers as well as the participants of this prestigious National Conference, a
great success and hope that ideas emanating from the Seminar will serve to set new frontiers of
knowledge in the subject.

(Dr. Aseem Chauhan)

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

Convener, NCDDFD 2016
Dy. Director, Amity Institute of Pharmacy
Amity University, Lucknow Campus
Message
It is matter of great pride that the team Amity Institute of Pharmacy is organizing a 3 rd national
scientific event. Drug Discovery with pace is need of hour in current scenario. We are fighting
with deadliest existing diseases and new outbreak like Zika virus infection. The researchers
across the world have no other choice but to work together. The budding students and
researchers need exposure to newer technology and scientific finding.
I am sure that National Conference on Drug Discovery and Formulation Development will serve
its important role in giving a common platform for new generation to interact and share ideas
with eminent scientist and academician. I welcome all the delegates to AMITY and thank you for
making this conference a great success.

(Dr. Sajal Srivastava)

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

Parmesh K. Dwivedi
(Treasurer-NCDDFD 2016)

I am extremely delighted to greet and welcome you all to National conference on “Drug
discovery and formulation development”. Theme of the conference has much significance as the
drug discovery and formulation development are the heart of pharmaceutical research. Now a
days, the process for research and development of new medicines is growing in difficulty and
length. Hence, the goal of this conference is to bring together researchers, academicians, industry
persons and learners to exchange their research ideas and results. We look forward to an

exciting day of insightful presentations, discussions and sharing of technical ideas with
colleagues from different parts of our country.
I hope and expect that the theme will result in fruitful and passionate discussions.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

Responsibility Matrix:
S. Committee
No Name
.
1 Overall
Coordinator

2 Marketing

3 Invitation
4 Hospitality

5 MC

6 Transport

7 Reception

8 Hall
Management

Faculty Incharge/
Co-incharge

Student Name

Programme

Phone No.

Dr. Sajal Srivastava

Ms. Priyanka
Srivastava; Ms.
Shivani Singh

B. Pharm

9839030220
9415054170

Dr. Sajal Srivastava,
Mr. Parmesh K
Dwivedi, Dr. Rahul
Shukla
Dr. M V Ramana, Dr.
Sajal Srivastava
Mr. Amrit K Singh Mr.
Prakash Deep

Ms. Priyanka
Srivastava

B. Pharm

9839030220

Mr. Shushant
Mishra and Mr.
Anshu Singh
1. Ms. Priyanka
Srivastava Ms
Alka Rai; 2. Parth,
Abhishek3. Mr.
Anurag

B. Pharm 6th
sem

8853933214

Mr. Shunbam
Pandey Mr. Rahul
Kushwaha
Mr. Vikas
Upadhyay
Ms. Richa
Srivastava;
Ms. Nishita and
Ms Komal
Akansha lal
Ms. Sristi raj and
Shreya
Pooja Yadav

B. Pharm

Mr. Saikat Sarkar

Mr. Rahul Shukla

Ms Nimisha

Dr . Zeeshan Fatima;
Dr. Himani Awasthi

B. Pharm
B Pharm

8953591757
9839030220

M Pharm
9616511037

8174838048
Ph. D.
B. Pharm

9936824032

B Pharm

8102734585

B. Pharm 8th
sem

7398517685

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

9 Disciplinary

Mr. P. P. Dash; Ms.
Richa Srivastava

10 Registration

Ms Nimisha

11 Accommodati
on

Ms. Neha Mathur and
Mr. Saikat Sarkar

12 Poster Display

Dr. Mohini Chaurasia

13 Certificate

Mr. P K Dwivedi

Mr. Rahul Pal and
Mr. Abhishek
Pandey; Ankan
Rastogi and
Shubham
Kesarwani; Mr.
Himanshu Jaiswal
and Mr. Prasoon
Dwivedi
Ms. Richa
Srivastava;
Ms. Nishita and
Ms Komal

B. Pharm

8726982698
9044999769
8090225216

Ravindra,
B. Pharm 8th
Rajeshwar and
sem
vimal
Ms. Shivani Singh B. Pharm 8th
Ms Akansha Lal
sem

9807801157
9795910460
8960239311
9415054170
8899309635

B. Pharm 6th
Sm

7398221508

B. Pharm

9688623489

B. Pharm

7688623419

14 Saraswati
vandana

Dr. Sajal Srivastava

15 Printing,
Publishing
Media & Press
16 Abstract
Screening
& Printing

Dr. Sajal Srivastava,
Mr. Ashutosh Chaubey

Mr. Vidhu
shekhar Dixit
Mr. Zeeshan
Ahmad
Ms. Mini Singh;
Ms Ankita Singh;
Ms. Kashish
Kapoor
Ms. Rahul
Kushwaha

Dr. Sajal Srivastava

Mr. Himanshu

B. Pharm VI

8765812332

17 Rapporteur

Ms. Neha Mathur; Ms.
Suchita

Mr. Vimal Misra

B. Pharm 8th
sem

8960239311

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

SL.
AUTHORS
POSTER
NO.
NO.
,
LUBNA AZMI , ILA
PO301
1
SHUKLA, SHYAM
SUNDAR GUPTA,
PADAM KANT,
CH.V.RAO
SANGEETA BAJPAI, PO302
2
D.K.AWASTHI

TITLE
ULCER PROTECTIVE EFFECT OF ARGYREIA
SPECIOSA METABLOLITES ON PROBIOTIC
BACTERIA

FORMULATION OF XANTHINE IN THE
TREATMENT OF BRONCHITIS

3

RICHA
SRIVASTAVA,
SAJAL
SRIVASTAVA

4

PO304
PRITT VERMA,
SHRAVAN K.
PASWAN, SURYA P.
SINGH, SAJAL
SRIVASTAVA, CH
V.RAO1

PROTECTIVE EFFECT OF ACACIA NILOTICA
(BARK) AGAINST ANTI TUBERCULAR DRUG
INDUCED HEPATIC DAMAGE AN
EXPERIMENTAL STUDY

5

PO305

ANTIOXIDANT AND WOUND HEALING
PROPERTIES OF PHYLA NODIFLORA AGAINST
EXCISION WOUND HEALING ACTIVITY

PO306

ANTI HEPATOTOXIC ACTIVITY OF MORUS
ALBA IN RAT MODEL OF ALCOHOL LIVER
DISEASE

7

SHRAVAN K.
PASWAN, PRITT
VERMA, ALOK
R.GAUR, ABHISEK
RAJ, CH.V. RAO,
SAJAL
SRIVASTAVA
ILA SHUKLA,
LUBNA AZMI,
SHYAM SUNDAR
GUPTA, PADAM
KANT, CH.V.RAO
MARYAM SARWAT

PO307

8

MADAN SWATI

PO308

9

NESAR AHMAD,
NOORUL HASAN,
ZEESHAN AHMAD,
ARUN KUMAR,
SHEIKH
ZOHRAMEENA

PO309

LAMP BASED APPROACHES FOR
AUTHENTICATION OF TRIBULUS TERRESTRIS
PHYTOCHEMICAL ANALYSIS AND FREERADICAL SCAVENGING ACTIVITY OF
FLACOURTIA INDICA (BURM.F.) MERR.
DICLOFENAC SODIUM: TREATMENT OF
RHEUMATIC DISEASES

10

NOORUL HASAN,

PO310

6

PO303

LIPOSOMAL DRUG DELIVERY OF
METHOTREXATE

INTRANASAL DELIVERY: AN APPROACH TO

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

11

12

13

14

15

16

17

NESAR AHMAD,
ZEESHAN AHMAD,
ARUN KUMAR,
SHEIKH
ZOHRAMEENA
FURRUKH AHMAD
ZEESHAN AHAMD,
ARUN KUMAR,
KULDEEP SINGH,
S.P. SINGH, MD.
ZISHAN,
PARAMDEEP
BAGGA
MOHAMMAD
AMIR, ZEESHAN
AHMAD, MD.
ZISHAN, ARUN
KUMAR, KULDEEP
SINGH, S.P. SINGH
MOHAMMAD
ZISHAN, ZEESHAN
AHMAD, VASEEM
A. ANSARI, S. P.
SINGH, FAISAL
SAEED,
MOHAMMAD AMIR
SAHAR IDRIS,
AIJAZ AHMAD, MD.
ZISHAN, ARUN
KUMAR,
MOHAMMAD
AMIR, NAZMA
KHAN
ASHUTOSH
PATHAK,
MITHILESH
KUMAR,
HIMANSHU
MISHRA
NEHA MATHUR,
RAVI KUMAR,
KANKSHI TIWARI,
SUPRIYA SINGH,
NIKHAT FATIMA
SAURABH

BYPASS THE BLOOD BRAIN BARRIER

PO311

NANOCOSMETICS: AN OVERVIEW

PO312

PULMONARY DRUG DELIVERY

PO313

RECENT TRENDS IN RECTAL DRUG
DELIVERY SYSTEM

PO314

TARGETED DRUG DELIVERY TO CNS USING
NANOPARTICLES

PO315

CANCER VACCINE: AN INNOVATIVE
APPROACH IN CANCER TREATMENT

PO316

EVALUATION OF QUALITY CONTROL
PARAMETERS ON VARIOUS BRANDS OF
PARACETAMOL TABLET FORMULATION

PO317

AN EXCELLENT VEHICLE FOR NOVEL DRUG

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

18

SRIVASTAVA,
SHADAB
MOHAMMAD,
SANA FAROOQUI,
DEVENDRA
KUMAR
KARUNA S.
SHUKLA, MONIKA,
SHAILENDRA
PANDEY, POOJA
CHAWLA

DELIVERY SYSTEM: BIODEGRADABLE
POLYMERIC NANOPARTICLES

PO318

THIAZOLIDINE-2, 4-DIONE & ITS ANALOGUES
AS ANTICANCER MEDIATED BY DEPLETION
OF INTRACELLULAR CALCIUM: A REVIEW

DNA FINGERPRINTING OCIMUM BASILICUM
AND O. SANCTUM THROUGH RAPD MARKERS
SIMULTANEOUS ESTIMATION OF
PIOGLITAZONE AND LOVASTATIN BY RPHPLC

19

MARYAM SARWAT

PO319

20

MONIKA, KARUNA
SHANKER
SHUKLA,
SHAILENDRA
PANDEY, POOJA
CHAWLA
MAYANK
KULSHRESHTHA,
MANJUL PRATAP
SINGH
GUNJA
SRIVASTAVA,
MANJUL PRATAP
SINGH, ANURAG
MISHRA
SHAISTA SUHAIL,
KUMUD NIGAM,
SAURABH
SRIVASTAVA
M.V. RAMANA,
KAMAL DUA

PO320

SHUBHAM
SHARMA, NIMISHA
MISHRA
SATYENDRA K
RAJPUT,
VARSHALA PAL,
ARUN K SHARMA,
SHYAM SUNDAR
AGRAWAL
PRATIBHA GUPTA,
ANUPUM KUMAR

21

22

23

24
25
26

27

PO321

PO322

DOUBLE PYLORUS: AN UNCOMMON
FEATURE OF STOMACH

TRITERPENOIDS: A POTENT
HEPATOPROTECTIVE AGENT

PO323

CURCUMIN AS A DRUG IN TREATMENT OF
ORAL CANCER

PO324

FORMULATION AND EVALUATION OF
METOPROLOL TARTRATE MICROSPHERES

PO325

MODEL-BASED DRUG DEVELOPMENT:
APPLICABLE TO ONCOLOGY

PO326

EFFECT OF BUXUS WALLICHIANA BAILL
EXTRACT AGAINST EXPERIMENTAL
INDUCED AMNESIA IN SWISS ALBINO MICE

PO327

FORMULATION DEVELOPMENT &
CHARACTERIZATION OF ETHOSOMAL GEL

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

28

29

30
31

32
33
34
35
36

37

38

39

SACHAN

APPROVED FOR THE TREATMENT OF
ARTHRITIS

MRIDUL RAJEEV,
PO328
NIKHAR SHUKLA,
MONIKA KAMBOJ
AND RICHA KHARE
NIKHAR SHUKLA,
PO329
MRIDUL RAJEEV,
SMRITI KHARE
AND RICHA KHARE
UPASANA YADAV
PO330

NANO PARTICLES IN DRUGS

IMPACT OF CHITOSAN NANOPARTICLE IN
DRUG DELIVERY
CHEMICAL CONSTITUENTS AND PARTS OF
PLANTS AS IMMUNOMODULATORS

KALPANA
SONWANI,
ASHUTOSH
KUMAR YADAV
POOJA RAO,
DHARAMVEER

PO331

PO332

“CLINICAL BENEFITS OFHERBAL BASED
ADAPTOGENS ”

RICHA MISHRA,
ASHUTOSH
KUMAR YADAV
JYOTI GUPTA,
MAYANK
KULSHRESHTA
GARIMA SINGH,
DHARAMVEER
SARASVATEE
KUMARI,
ASHUTOSH
KUMAR YADAV
KHUSHABOO
SINGH,
DHARAMVEER,
MAYANK
KULSHRESHTHA
DEEPA SHUKLA,
SAJAL
SRIVASTAVA,
TALHA JAWAID
SACHIN NEEKHRA,

PO333

ANIMAL MODELS FOR THE STUDY OF STRESS
VULNERABILITY RESILIENCE

PO334

NATURAL MEMORY BOOSTERS IN INDIAN
HERBAL PLANTS

PO335
PO336

CHRONIC UNPREDICTABLE STRESS:A
CLASSICAL MODEL OF DEPRESSION
FLAVONOIDS AS AN ANALGESIC,
ANTIPYRETIC AND ANTI-INFLAMMATORY
AGENT

PO337

ROLE OF Β-AMYLOID PROTEIN IN
ALZHEIMER DISEASE

PO338

A REVIEW ON EFFECT OF ANXIETY AND
STRESS ON MEMORY

PO339

EVALUTION OF HEPATOPROTECTIVE
POTENTAIL OF ALCOHOLIC AND AQUEOUS
EXTRACT OF TERMINALIA BELERICA IN RATS
MECHANISTIC APPROACHES OF DIFFERENT

ROHIT VERMA
40

ROLE OF BIOTECHNOLOGY IN
PHARMACEUTICALS

DAN BAHADUR
ROKAYA, HIMANI

PO340

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

MODELS OF ALZHEIMER’S DISEASE

AWASTHI
41

UPASANA YADAV,
ARCHNA TIWARI

PO341

METAL–ORGANIC-FRAMEWORK
NANOPARTICLE AS A POTENTIAL FOR DRUG
DELIVERY AND IMAGING

42

PO342

WILDLY GROWING PLANTS AS A SOURCE OF
AN ALTERNATE MEDICINE: A POSSIBLE
DRUG DEVELOPMENT STRATEGY

43

CHANDNI
TANDON, PRITI
MATHUR AND
MANODEEP SEN
MONA PAL

PO343

JAPANESE ENCEPHALITIS

44

SANNI GANGWAR

PO344

ANTIVIRAL & ANTIRETROVIRAL

45

PARUL TRIPATHI,
PARUL JOHRI,
ADITI SINGH
SARITA JHA,
PARUL JOHRI,
MALA TRIVEDI
RUCHI YADAV,
A.B.PANT, PRACHI
SRIVASTAVA

PO345

ATOMIC LEVEL ANALYSIS OF ENVELOP
PROTEIN FROM DIFFERENT STRAINS OF HIV
VIRUS
PROTEOME WIDE INTERACTION ANALYSIS

46

47

48

49

50

PO346

OF MITOCHONDRIAL PROTEINS
PO347

CHETAN RASTOGI, PO348
MOHD.MUDDASSIR
KHAN, SHRAVAN
PASWAN, PREET
VERMA, ALOK
RANJAN GAUR,
CH. V. RAO, SAJAL
SRIVASTAVA
MOHD.
PO449
MUDDASSIR
KHAN, CHETAN
RASTOGI , PREET
VERMA, SHRAVAN
PASWAN, ALOK
RANJAN GAUR, CH.
V. RAO,. SAJAL
SRIVASTAVA
SHIKHAR VERMA,
PO350
ABHISHEK GUPTA,
M.V. RAMANA, A.K.S
RAWAT

MOLECULAR DOCKING OF RESVERATOL
WITH HIGH THROUGHPUT
NEURODEVELOPMENT INJURY DATA
UNLOCKS POTENTIAL DRUG TARGETS
EFFECT OF DESMODIUM GANGETICUM ON
ETHYLENE GLYCOL INDUCED
NEPHROLITHIASIS IN RATS

EFFECT OF BERBERIS ARISTATA ON
STREPTOZOTOCIN INDUCED DIABETIC IN
RATS

HPTLC ANALYSIS FOR THE QUANTIFICATION
OF GALLIC ACID AND QUERCETIN IN

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

METHANOLIC FRACTION OF LIMONIA
ACIDISSIMA L. FRUITS
51

52

ANKAN RASTOGI,
MUSAB RAFI,
HIMANSHU
JAISWAL
SHWETA SACHAN,
PARUL JOHRI, ADITI
SINGH

PO351

AN ABSTRACT ON NATURAL ANTIOXIDANTS

PO352

GLYCOSYLATION IN LIPASE ENZYME OF
HOMO SAPIENS AND ITS CORRELATION
WITH CARBON COMPOSITION

53

54
55

JYOTI WAGH,
SANDEEP,
ASHUTOSH MISHRA,
RAHUL SHUKLA,
SAIKAT SARKAR,
SUMIT GUPTA
ASHUTOSH MISHRA,
RAHUL SHUKLA,
SANDEEP
ANKITA TRIPATHI,
SURAJ NEUPANE

PO353

YOGA: LIFE OF SCIENCE

PO354

PREVENTIVE MEASUREMENTS TO STRESS IN
CURRENT ENVIRONMENT

PO355

A REVIEW ON NECROTIZING FASCIITIS-A
LIFE THREATNING INFECTION OF SKIN AND
SOFT TISSUES

56

SANDEEP, ANGSHU
BANARJEE,
ABHILASA, RAHUL
SHUKLA,
ASHUTOSH sMISHRA

PO356

EVALUATION OF PERIPHERAL ANALGESIC
ACTIVITY OF BARK OF ANNONA SQUAMOSA
FROM GONDA REGIONs

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

Invited talk
" An introduction to Drug Discovery by rational approach and
applications of Green Chemistry in Drug Synthesis"
Prof. Dr. J. Saravanan, Principal,
PES College of Pharmacy,50 feet road,
Hanumanthnagar,Bangalore- 560 050,
email : drjayes@gmail.com.

Abstract
Rational drug discovery is a more streamlined process that requires careful consideration of the
target of the drug as well as the drug itself. This method of drug design uses special equipment to
examine the three-dimensional structure of a drug target and then find a compound that can
interact with the target. Rational drug design therefore requires sound knowledge of chemistry as
well as biology, because chemical interactions between drugs and their targets are what
determine whether a drug is biologically active.

Discovery of new drugs by serendipity and random screening is no more appropriate, instead
drug discovery by rational approach involving techniques like bioisosterism , enzyme inhibition
, consideration of Lipinski rule of five and so on.
The term “green chemistry” is defined as “the invention, design and application of chemical
products and processes to reduce or to eliminate the use and generation of hazardous
substances”. Green chemistry can diminish the need for other approaches to environmental
protection. Ideally, the application of green chemistry principles and practice renders regulation,
control, clean-up, and remediation unnecessary, and the resultant environmental benefit can be
expressed in terms of economic impact.

Microwave assisted organic synthesis has revolutionized organic synthesis. Small molecules can
be built in a fraction of the time required by classical thermal methods. As a result, this
technique has rapidly gained acceptance as a valuable tool for accelerating drug discovery and
development processes.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO301
ULCER PROTECTIVE EFFECT OF ARGYREIA SPECIOSA METABLOLITES ON
PROBIOTIC BACTERIA
Lubna Azmi1*,, Ila Shukla2, Shyam Sundar Gupta1, Padam Kant, Ch.V.Rao
1

Pharmacognosy and Ethnopharmacology Division, CSIR-NBRI, Lucknow, India
2
University of lucknow , Lucknow, Uttar Pradesh, India
E-mail: azmilubna@gmail.com

In the present study, the 50 % w/w ethanolic extract of natural occurring medicinal plant
Argyreia speciosa (L.f), sweet (Family Convolvulaceae) was studied for antioxidant, antiulcer
activity and its purified secondary metabolites were assessed for growth promoting effects on
probiotic bacteria Lactobacillus casei. The gastric ulcers were significantly decreased by all
doses of ASE and probiotic combination as compared with the indomethacin (25 mg/kg body
weight) treated group. In the stomach tissues of treated animals, antioxidant levels were
examined. The administration of indomethacin caused a significant decrease in the levels of
superoxide dismutase (SOD), glutathione peroxidise (GPx) and reduced glutathione (GSH), and
a raise in the lipid peroxidation (LPO) level (p < 0.05). The administration of all doses of ASE
reversed the trend, call to mind a significant increase of SOD, GSH and GPx levels and a
reduction in LPO level in tissues. However, catalase (CAT), glutathione reductase (GR) and
myeloperoxidase (MPx) activities, increased by indomethacin, were found to be lower in the
ASE, probiotic combination of ASE + L.casei and omeprazole-treated groups. The gastric
mucosal constitutive NO synthase (cNOS) and inducible NO synthase (iNOS) activities were
also investigated in tissues of ASE (100 mg/kg), ASE (100 mg/kg) + L. casei (10-8 con.),
omeprazole and indomethacin-treated rat groups. The administration of ASE + L.casei and
omeprazole increased the cNOS activity and lowered the iNOS activity as compared with
indomethacin-treated group. As far as the growth promoting effects of ASE metabolites on L.
casei is concerned, querecetin showed high growth stimulating activity in increased dry matter of
biomass. At low pH growth promoting activity of metabolite was found stable.

Keywords: Argyreia speciosa, Antioxident activity, Antiulcer activity, Indomethacin

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO302
FORMULATION OF XANTHINE IN THE TREATMENT OF BRONCHITIS
Sangeeta Bajpai*1, D.k.Awasthi2
1
2

School of Applied Sciences Amity University Lucknow U.P.

Department of Chemistry Sri J.N.P.G. College Lucknow U.P.
Email: dkawasthi5@gmail.com

Methylxanthines are used for the preparation of group of drugs. These are playing very important
for smoothing of bronchial muscles and stimulate central nervous system (CNS).The
bronchodilator effects of such drugs are applied in the treatment in acute asthma. Such drugs are
also used in the treatment of aponea and have diuretic effect. Oxpentifylline, is a Xanthine
derivative acts as vasodilator. Naturally occurring are caffeine, theophylline and theobromine.
Their derivatives can be used in the improvement form for the treatment of asthma. Modified
forms are Diprophylline, Etamiphylline Camsylate, Proxyphylline and Hydroxytheophylline,
Etofylline. They can be prepared and sell into the market as tread names. Aminophylline,
Aminophylline Hydrate, Choline Theophyllinate. Theobromine is also used in the treatment of
angina pectoris and hyper tension. The basic nucleus is xanthine. It can be substituted by various
groups for the preparation more effective drugs. Now days air pollution is fast growing and
Indian citizens are suffering from respiratory problems. More emphasis is required on bronchial
drugs and treatment of bronchitis, asthma, emphysema and acute respiratory infection .we feel
among such drugs Aminophylline would be better. It can be given orally as well as through
injection.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO303
LIPOSOMAL DRUG DELIVERY OF METHOTREXATE
Richa Srivastava*, Sajal Srivastava
Amity Institute of Pharmacy, AUUP, Lucknow Campus
Various advanced drug delivery systems such as nanoparticle which include polymeric micelles,
dendrimers , liposomes, SLN, have been investigated to overcome the limitation of the
conventional systems. Considering effectiveness of drug therapy of methotrexate (MXT), the study
focused on formulation of liposomes. The objective of this study was to achieve desired
entrapment of methotrexate in liposome. Liposomes were prepared by thin film hydration method
The liposome were prepared and characterized by, zeta potential for surface charges and particle
size analysis done by Malvern nanosizer and average particle size and zeta potential was found
to be 169 nm and -16.9mV respectively. Liposomal formulations as carrier system for MTX
destined for treatment of cancer by passive targeting Dynamic in vitro drug release was carried
out using phosphate buffer pH 7.4. A short term stability studies was conducted for 2 months at
40C and at room temperature (250C). Final formulations were prepared after lyophilizing the
selected liposomes.. MTX entrapped in the liposomes and the entrapment efficiency was found
to be 83 %. The drug loading and entrapment efficiencies were evaluated by a HPLC method
(C18 column 5m, 150mm×4.6mm,50mM ammonium acetate/acetonitrile mobile phase v/v 3.0
ml/min flow rate and 256 nm UV detection).The evaluation studies of different processing
variables like drug to lipid ratio, soya lecithin to cholesterol ratio, temperature were evaluated .

KEY WORDS: Liposomes, lyophilization, drug release.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO304
PROTECTIVE EFFECT OF ACACIA NILOTICA (BARK) AGAINST ANTI
TUBERCULAR DRUG INDUCED HEPATIC DAMAGE AN EXPERIMENTAL STUDY
Pritt Verma*1,2, Shravan K. Paswan1,2, Surya P. Singh1, Sajal Srivastava2, Ch V.Rao1
1

Pharmacognosy and Ethnopharmacology Division, CSIR- BRI, Lucknow, India.
2

Amity Institute of Pharmacy, Amity University, Lucknow Campus, India.

Rats were divided into five different groups (n=6), the group I served as a control, Group II
received Isoniazid-INH and rifampicin-RIF (50mg/kg) in sterile water, group III and IV served
as a treatment and received 200,400 mg/kg of 50% ethonolic extract of A. nilotica, and group V
served as standard group and received silymarin (100mg/kg). All the treatments were given for
10-28 days and after rats were authorized, blood and liver were collected for biochemical and
histopathological studies, respectively. The 50% ethanolic bark extract of A. nilotica (200, 400
mg/kg p.o.) showed the remarkable hepatoprotective effect against Isoniazid-INH and
rifampicin-RIF induced hepatic damage, and observed that it shows no any significant change in
a normal posture, behavior and body weight in Wistar rats. The degree of protection was
measured by biochemical and antioxidant parameters such as serum glutamate oxaloacetate
transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase
(ALP), total bilirubin, and the histopathological profile of liver also indicated the
hepatoprotective nature of this drug. The bark extract of A. nilotica has showed dose dependent
activity, among which at the dose level of 200 & 400 mg/kg. The further investigations, the bark
extract of A. nilotica identify the active constituents responsible for hepatoprotection.
Keywords: A. nilotica, Antioxidant, Isoniazid-INH, Rifampicin-RIF, Silymarin.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO305
ANTIOXIDANT AND WOUND HEALING PROPERTIES OF PHYLA NODIFLORA
AGAINST EXCISION WOUND HEALING ACTIVITY
Shravan K. Paswan1,2*, Pritt Verma1,2, Alok R.Gaur1, Abhisek Raj1, Ch.V. Rao1, Sajal
Srivastava2
1

Pharmacognosy and Ethnopharmacology Division, CSIR- BRI, Lucknow, India.
2
Amity Institute of Pharmacy, Amity University, Lucknow Campus, India.

Leaf extract of Phyla nodiflora was developed as ointment (5% & 10% w/w) with easy ointment
base B.P. The ointment was then evaluated for excision wound healing activity. Parameters as
well as antioxidant, measurement of wound space &, wound contraction index, a measurement of
tensile strength and histopathological examination content were determined. Remarkable wound
healing activity was observed with the 10% (w/w) ointment of 50% ethonolic leaf extract of
Phyla nodiflora. Statistical analysis was performed by one-way analysis of variance followed by
t-test. Wound treated with 5% and 10% (w/w) 50% ethonolic leaf extract ointment exhibited
significant excision wound and antioxidant parameter, the healing activity of the excision wound
as evidenced by increased wound contraction, shorter epithelization time, higher tissue breaking
strength and increased hydroxyproline content. This plant has important biological activities and
responsible for the antioxidant and wound healing properties. The study provided sufficient
evidences that Phyla nodiflora might be indeed potential sources to treat many diseases.
Keyword-: Phyla nodiflora, Antioxidant, Histopathological, wound-space, wound contraction.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016
PO306

ANTI HEPATOTOXIC ACTIVITY OF MORUS ALBA IN RAT MODEL OF ALCOHOL
LIVER DISEASE
Ila Shukla1*, Lubna Azmi2, Shyam Sundar Gupta1, Padam Kant2, Ch.V.Rao1
1

Pharmacognosy and Ethnopharmacology Division, CSIR-NBRI, Lucknow, India
2
University of lucknow , Lucknow, Uttar Pradesh, India
E-mail: ilashukla069@gmail.com
Alcoholic liver disease has a known aetiology but a complex and incompletely known
pathogenesis. It is an extremely common disease with significant morbidity and mortality.
Therapy for treatment of it comes with lot of side effects. Presently there is a need of some
natural therapy with lesser side effects and more potential to treat the disease.
Present study is designed with an objective to investigate the antioxidant and
hepatoprotective activities of the ethanolic extract of the Morus Alba in rat model of alcohol
liver disease. Rats were divided into four groups of six each. Group-1 (control) is isocaloric
pairfed, Group-2 received ethanol at a dose of 6g/kg/day, Group- 3 and 4 were administered
extract of Morus Alba as an aqueous suspension orally along with alcohol. After eight weeks,
liver weight, liver function serum markers such as alkaline phosphatase (ALP), aspartate
transaminase (AST) and alanine transaminase (ALP) and lipid peroxidation were measured.
Aqueous suspension showed significant decrease in the levels of AST, ALT and ALP as
compared to alcohol-fed rats. Extract of Morus Alba ameliorated alcohol induced liver injury.
Keywords : Morus Alba, Hepatoprotective, Alcohol Liver Disease, Antioxidant, lipid
peroxidation.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO307
LAMP BASED APPROACHES FOR AUTHENTICATION OF TRIBULUS TERRESTRIS
Maryam Sarwat*
Pharmaceutical Biotechnology, Amity Institute of Pharmacy, Noida, UP – 201303
E mail: maryam21_7@yahoo.com; msarwat@amity.edu
Plant based medicines are popular worldwide due to their negligible side effects. There is no
control over the quality of the raw material for medicinal plant use. There are various incidences
of adulteration of medicinal plants with useless weeds. Keeping this in mind, it is imperative to
authenticate the plant material before being used.
Our study plant Tribulus terrestris (Zygophyllaceae), has utmost medicinal importance in curing
urinary discharges, cough, asthma, pain, spermatorrhoea, ophthalmia, anemia, dysentery, skin
and heart diseases; The original use for T. terrestris extract was as a ‘tonic’ to treat sexual
dysfunction. The plant get often confused with Kallstroemia parviflora and K. californica,
because of their similar morphology. These plants, often get accidentally mixed with T. terrestris
while collecting raw material for herbal drug preparations, making them less efficient or some
times toxic. Samples of this plant were collected from different geographical locations in India.
Random amplified polymorphic deoxyribonucleic acid (DNA) analysis of collected samples was
carried out with 24 random primers. A 510-bp DNA fragment, common to all accessions, was
eluted, cloned, and sequenced. Four LAMP primers were designed on the basis of sequence of
510 bp DNA fragment. LAMP reaction was performed at 65°C for 1 h. The resulting amplicon
was visualized through SYBR Green I. LAMP using a primer set for T. terrestris and total DNA
extracted from T. terrestris leaves (0.5—10.0 ng) as template was detected up to 70 min. On the
other hand, in the reaction using a primer set for T. terrestris and total DNA from K. parviflora
as template, no amplifications were detected. The same tendency could be seen in the reactions
using a set of primers for K. parviflora. LAMP enabled not only identification but also detection
with high specificity. The data showed confirmatory results. Since the assay method is simple,
sensitive, and cost-effective, it is a feasible method for identifying and authentication of C.
roseus.
Keywords: Medicinal plants, authentication, Tribulus terrestris, trnk, LAMP based markers.
Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO308
PHYTOCHEMICAL ANALYSIS AND FREE-RADICAL SCAVENGING ACTIVITY OF
FLACOURTIA INDICA (BURM.F.) MERR.
Madan Swati*
Amity Institute of Pharmacy, Amity University, Noida (U.P), India
Flacourtia indica (Burm.f) Merr. Syn. F. ramontchi L Herit (Flacourtiaceae) is a small spinous
tree or shrub found distributed throughout the Himalayas, northern districts of Uttar Pradesh,
Assam, Bengal and Orissa. The roots and fruits of F. indica are used in traditional medicine as a
diuretic, hepatoprotective and antidiabetic. Flacourtia indica dichloromethane (DCM) stem bark,
butanol (BuOH) stem bark, methanol (MeOH) stem bark and MeOH leaf extract were screened
for in vitro antioxidant activity using different models. Subsequent quantification showed the
presence of 15.62 and 11.53 % w/w phenolics; 1.15 and 1.80 % w/w of flavonol in methanol
extract of stem bark and leaf of Flacourtia indica (F. indica) respectively. The methanolic
extract of stem bark and leaf of F. indica showed an effective DPPH radical scavenging activity
with low IC50 values of 17.5 and 21 µg/ml respectively. Butanolic extract of F. indica stem bark
showed nitric oxide radical inhibition activity with IC50 value of 28.5 µg/ml. The methanolic
extract of stem bark showed hydroxyl radical scavenging by p-NDA method with IC50 value of
350.23 µg/ml.
Key words: Flacourtia indica, DPPH, Nitric oxide radical inhibition assay, Scavenging,
hydroxyl radical, p-NDA

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO309
DICLOFENAC SODIUM: TREATMENT OF RHEUMATIC DISEASES
Nesar Ahmad*, Noorul Hasan, Zeeshan Ahmad, Arun Kumar, Sheikh Zohrameena
Integral University, Lucknow
E-Mail- nesar50@gmail.com
Diclofenac is the medicine works by reducing substances in the body that causes pain and
inflammation. Diclofenac used to treat mild to moderate pain, or signs and symptoms
of osteoarthritis or rheumatoid arthritis. The Cataflam brand of this medicine is also used to treat
menstrual cramps. Diclofenac 75 to 150mg daily (25 to 50mg 3 times daily) is comparable in
efficacy with ordinary aspirin 3 to 5g daily and indomethacin 75 to 150mg daily in rheumatoid
arthritis and with indomethacin in osteoarthritis Diclofenac powder (Cambia) is used to treat
a migraine headache attack. It will not prevent headaches or reduce the number of attacks not use
Diclofenac. If patient have a history of allergic reaction to aspirin or NSAIDs it can increase the
risk of fatal heart attack or stroke, especially if he use it long term or take high doses, if he
suffering from heart disease. Although there is some evidence of Diclofenac efficacy when
administered twice daily, or once daily as a slow release tablet. The drug is also available as
suppositories and ampoules for intramuscular injection. It is not recommended just before or
after heart bypass surgery (coronary artery bypass graft or CABG). These conditions can occur
without warning while you are using this medicine, especially in older adults. No one of the nonsteroidal anti-inflammatory agents is the most suitable drug for all patients requiring such
therapy, and Diclofenac should be considered along with other drugs of its type in the arthritic
patient.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO310
INTRANASAL DELIVERY: AN APPROACH TO BYPASS THE BLOOD BRAIN
BARRIER
Noorul Hasan*, Nesar Ahmad, Zeeshan Ahmad, Arun Kumar, Sheikh Zohrameena Furrukh
Ahmad
Faculty of Pharmacy, Integral University, Lucknow
E-Mail:- noorul636@gmail.com
The blood brain barrier (BBB) represents one of the strictest barriers of in- vivo therapeutic drug
delivery. The barriers are restricted exchange of hydrophilic compounds, small proteins and
charged molecules between the plasma and central nervous system (CNS). For decades, the BBB
has prevented the use of many therapeutic agents for treating Alzheimer’s disease, stroke, brain
tumor, head injury, spinal cord injury, depression, anxiety and other CNS disorders. The
emerging approach is to bypass the BBB by intranasal delivery, which provides a practical, noninvasive, rapid and simple method to deliver the therapeutic agents to the CNS. This method
works the unique connection between the nose and the brain that has evolved to sense odors and
other chemical stimuli. On the basis of clinical trials (Phase I and II) it is reported that the
intranasal route is feasible for the transport of the drug to the CNS. Intranasal delivery does not
require any modification of the therapeutic agents and does not require that drugs be coupled
with any carrier like in case of drug delivery across the BBB. A wide variety of therapeutic
agents, including both small molecules and macromolecules can be successfully delivered,
including to the CNS, using the intranasal method. Advantage of intranasal delivery are
Bypasses the BBB and targets the CNS, reducing systemic exposure and thus systemic side
effects and Avoids destruction in the gastrointestinal tract, hepatic “first pass” elimination and
gut wall metabolism, allowing increased, reliable bioavailability.
Key words: Alzheimer’s disease; Acetylcholine esterase, Buxus wullichiana; Locomotor,
Scopolamine, Donepezil

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO311
NANOCOSMETICS: AN OVERVIEW
Zeeshan Ahamd*, Arun Kumar, Kuldeep Singh, S.P. Singh, Md. Zishan, Paramdeep Bagga
Faculty of Pharmacy Integral University, Lucknow
E Mail: - zeeshanahmad2086@gmail.com
Platus, a roman philosopher words, “A woman without paint is like food without salt."
Nanocosmetics are any cosmetic containing nanoparticles. Nanoparticle is any entity with all
three external dimensions in the nanosize. The magnitude of size of particles in the range of 1 nm
to 100 nm is considered as nanoscale. “Nanocosmetics’’ was actually the first branch of
nanotechnology to be commercialized and marketed to consumers, according to Davis Baird.
Antiaging products are the most common example of nanocosmetics, with nanoparticles that go
beneath the surface of the skin and reduce the appearance of lines and wrinkles by reacting with
the body. Various forms of nanotechnology involved in cosmeceuticals like Nanoparticle,
Nanoemulsions, Bucky balls, Nanogel, Microgels Liposomes, Dendrimers, Cubosomes, Solid
Lipid Nanoparticles (SLN’s). Recent researches focusing on cosmeceutical products highlighted
strong growth perspectives in the coming years. According to them expanding at a rapid
compound annual growth rate of 7.7%, the global cosmeceutical market will reach $31.84 billion
by 2016. The global cosmeceutical market offers huge potential among the Asian countries, such
as Japan, China, and India which are set to attract major players in the future. Japan has already
made a remarkable position in the global cosmetics market and its position in the cosmeceutical
segment is effectively improving. A report, “Cosmeceuticals market to 2018,” forecasted that the
global cosmeceuticals market will reach $42.4 billion by 2018.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO312
PULMONARY DRUG DELIVERY
Mohammad Amir*, Zeeshan Ahmad, Md. Zishan, Arun Kumar, Kuldeep Singh, S.P. Singh
Faculty of Pharmacy, Integral University, Lucknow
Email: - mdamir2436@gmail.com
The respiratory tract is one of the oldest routes used for the administration of drugs. Over the
past decades inhalation therapy has established. It is a valuable tool in the local therapy of
pulmonary diseases such as asthma or COPD (Chronic Obstructive Pulmonary Disease). This
type of drug application in the therapy of these diseases is a clear form of targeted drug delivery.
Currently, over 25 drug substances are marketed as inhalation aerosol products for local
pulmonary effects and about the same number of drugs are in different stages of clinical
development. Pulmonary delivered drugs are rapidly absorbed except large macromolecules
drugs, which may yield low bioavailability due to enzymatic degradation and/or low mucosal
permeability. Pulmonary bioavailability largely depends on the physical properties of the
delivered protein and it is not the same for all peptide and protein drugs. Pulmonary drug
delivery is important researches are an impacts the treatment of illnesses including asthma,
chronic obstructive pulmonary disease and various diseases. Inhalation gives the most direct
access to drug target. In the treatment of obstructive respiratory diseases, pulmonary delivery can
minimize systemic side effects, provide rapid response and minimize the required dose since the
drug is delivered directly to the conducting zone of the lungs.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO313
RECENT TRENDS IN RECTAL DRUG DELIVERY SYSTEM
Mohammad Zishan, Zeeshan Ahmad, Vaseem A. Ansari, S. P. Singh, Faisal Saeed,
Mohammad Amir
Faculty of Pharmacy, Integral University, Lucknow U.P. India-226026
E mail: - zishanquadri786@gmail.com
There are many routs to deliver drugs into the body viz oral (through swallowing), sub mucosal
(through buccal and sublingual mucosa), parenteral (through injection), transdermal (through
skin), pulmonary (through inhalation) etc. Among these deliveries rectal routs is widely
accepted. Rectum delivery uses the rectum as a rout of administration for medication and other
fluids which are absorbed by the rectum’s blood vessels and flow into the body’s circulatory
system which distributes the drugs to the body’s organs. There are many advantages of the rectal
drug delivery system it avoidance of GI metabolism and degradation. The rectal route of
administration is useful for patients who cannot swallowed, Avoidance of irritation to the
stomach, partial avoidance of first pass metabolism, Rapid absorption of many low molecular
weight drugs. Prolonged effect, Absorption enhancement, Rate control drug delivery .There are
many drugs which are available in the market which are applied to rectal route like creams, gels,
suppositories, suspensions and rectal aerosols. Suppositories are available in two forms like solid
suppositories, liquid suppositories, Suppositories are medicated solid bodies suitably shaped for
rectal administration rectal drug delivery systems used the deliver the drug by using
mucoadhesive polymers in through the mucous of the rectum. Rectal drug delivery is so
medicated when the oral medication is not possible.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO314
TARGETED DRUG DELIVERY TO CNS USING NANOPARTICLES
Sahar Idris*, Aijaz Ahmad, Md. Zishan, Arun Kumar, Mohammad Amir, Nazma Khan
Faculty of Pharmacy, Integral University, Lucknow
Nano technology plays a unique and pivotal role in the development of brain specific drug
delivery, imaging and diagnosis. Targeted drug delivery is a means of concentrating dugs at a
specific site relative to other parts of the body. It spares the rest of the body from toxic effects of
the drug and is also a potential means of improving therapeutic index. The delivery of drugs to
the CNS is of prime importance for treating specific neurological disorders and various diseases
such as Meningitis, Encephalitis, degenerative diseases such as Alzheimer, Parkinson’s and
tumors such as Glioblastoma. The major problem in treating such CNS disorders is due to their
inability to surpass the natural CNS protective barriers, mainly the Blood Brain Barrier and the
Blood cerebrospinal fluid Barrier. Nanoparticle systems in CNS targeted drug therapy provide
better penetration of therapeutic and diagnostic agents, and a reduced risk in comparison to
conventional treatments. By using nanotechnology it is possible to deliver the drug to the
targeted tissue across the BBB, release the drug at a controlled rate, and avoid degradation
processes. Different types of nanoparticles used for CNS targeted drug delivery like inorganic
nanoparticles, Polymeric Nanoparticles, Solid Lipid Nanoparticles, Nano crystals, Carbon
nanotubes, Dendrimers, Quantum Dot’s, Gold Nanoparticles and Magnetic Nanoparticles.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO315

Benzothiazole: Different Methods of Synthesis and Diverse Biological
Activities
Desh Deepak Pandey*, Dr Dilip Kumar Pal
Rameshwarm Institute of Technology and Management Lucknow

Substituted 1, 3-benzothiazole derivatives are an important class of heterocyclic compounds. In
recent years heterocyclic compounds analogues and derivatives have attracted strong interest due
to their biological and pharmacological properties. The benzothiazole nucleus containing
compounds involved in research aimed at evaluating new products that possess biological
activities, such as antimicrobial, anticancer, antifungal, anthelmintic, anti-diabetic, amyloid
imagining agents and anticancer agents. The present review focus on the different methods of
synthesis of substituted benzothiazoles with potential activities that are now in developing phase.
Benzothiazole is a heterocyclic compound, weak base, having varied biological activities and
still of great scientific interest now a days. They are widely found in bioorganic and medicinal
chemistry with application in drug discovery. Benzothiazole moites are part of compounds
showing numerous biological activities such as antimicrobial anticancer anthelmintic antidiabetic activities.
Keywords: Substituted benzothiazole derivatives, antibacterial activity, anticancer activity, antidiabetic activity.
9
8

4

7

5
6

N
3

N
2

R1

R
S

S
1

1,3-benzothiazole
Chemicals and other reagents required for the synthesis will be procured from standard company
sources. Compounds will be synthesized by using standard procedures. The reactions will be
monitored by TLC and purification of the compounds will be carried out by recrystallization method
using suitable solvent. The synthesized compounds will be characterized by preliminary laboratory
techniques such as melting point, boiling point etc. Compounds synthesized will be confirmed by
FTIR, Mass Spectroscopy and NMR spectral data.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO316
CANCER VACCINE: AN INNOVATIVE APPROACH IN CANCER TREATMENT
Ashutosh pathak*, Mithilesh Kumar, Himanshu Mishra
Kamla Nehru Institute of Management & Technology, Sultanpur (U.P)
Cancer became a big question for scientific community as no existing treatments could
solve this dreadful disease. Research is in well progress since half century but it failed to give a
right solution to fight against it. One such mile stone treatment for cancer that is giving good
hope to the people is cancer vaccines. Cancer vaccines are made from the person’s own cancer
cells or from cells that are grown in a laboratory. Tumor vaccines are often based on DNA
construct viral vectors and cytokines that have been determined as safe. From previous clinical
trials Peptide vaccines are generally safe so long as the cytokine adjutants are used in
combinations and doses previously determined to be safe. A patient with pulmonary metastasis
of colon cancer was treated with artificially synthesized helper/killer-hybrid epitope long peptide
(H/K-HELP) of MAGE-A4 cancer antigen. The patient was vaccinated with MAGE-A4-H/KHELP combined with OK432 and Montanide ISA-51. There were no severe side-effects except
for a skin reaction at the injection site.
Antigen based tumor vaccines do not involve the insertion of modified tumor cell or immune cell
into the body. Instead, purified tumor proteins (antigens) are injected to stimulate the patient’s
antigen presenting cells (APCs) to take up the antigen and present it to T cells.
Keywords: Antigen presenting cells (APCs), cytokines, Peptide vaccines, Montanide

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO317
EVALUATION OF QUALITY CONTROL PARAMETERS ON
VARIOUS BRANDS OF PARACETAMOL TABLET FORMULATION
*

Neha Mathur, Ravi Kumar, Kankshi Tiwari, Supriya Singh, Nikhat Fatima

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow, India.
Paracetamol (Acetaminophen) is a widely used OTC medicine available as compressed tablets. It is
marketed and manufactured by a number of pharmaceutical companies and is widely used as an
analgesic, antipyretic medicine. In the present study we selected three brands of Paracetamol tablets
(Brand X, Brand Y and Brand Z) which are easily available and commonly used for analgesic and
antipyretic action. Further, the purpose of the present study was to perform the various quality
control tests of the compressed tablets like, weight variation, hardness, friability, disintegration and
dissolution as per the official monographs B.P, U.S.P and evaluate these three different brands. As a
result of this study we found that all the three brands met the specifications laid down in the

official monographs. They differ only slightly in terms of various quality control parameters,
brand Z disintegrated faster, had faster dissolution rate and so its % drug release was more than
the other two brands at the same time.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO318
AN EXCELLENT VEHICLE FOR NOVEL DRUG DELIVERY SYSTEM:
BIODEGRADABLE POLYMERIC NANOPARTICLES
Saurabh Srivastava1*, Shadab Mohammad1, Sana Farooqui1, Devendra Kumar2
1

Department of Oral Pathology & Microbiology, King George’s Medical University Lucknow,
Uttar Pradesh, India
2
Department of Pharmacology and Therapeutics, King George’s Medical University, Lucknow,
Uttar Pradesh, India
Email: saurabhadd09@gmail.com
Nanotechnology is new concept, it has been included biomedical, drug delivery, diagnostic,
imaging and mainly focusing in treatment and diagnosis of cancer & other disease state.
Nanoparticles (NPs) have unique and specific properties in terms of chemical and biological by
their small size ranges (1-100 nm) to have a surface area to volume ratio, which allows them to
combine, absorb and hold other compounds like drugs molecules, DNA, RNA, proteins.
Furthermore, their small size, shape and surface and other favourable characteristics helps the
drug therapy to become more stable, targeted, carrier orientated and incorporate with both
hydrophilic and hydrophobic substances. Biodegradable polymeric nanoparticles are the carriers
or vehicle where therapeutic agents can be encapsulated in their matrix and conjugated for
targeted delivery of a number of drugs. The site specific properties of biodegradable polymer as
nanoparticles reduce local pH and affecting cells microenvironment. There are many
biodegradable polymers use for drug delivery and classified synthetic, examples are poly (lactic
acid), poly (caprolactone), poly (glycolic acid), poly (lactic- co- glycolic acid), poly
(trimethylene carbonate), poly (butylenes succinate), poly (p-dioxanone), poly (butylenes
terephthalate), degrapol, hybrane, poly [(caboxyphenoxy) propane-sebacic acid], poly [bis
(hydroxyethyl) terephthalate-ethyl orthophosphorylate terephthaloyl chloride], poly (ethylene
glycol), poly (ortho esters), tyrosine derived, polycarbonate and semi-synthetic polymers are
poly (β- hydroxyalkanoate), poly (hydroxybutvrate), poly (hydroxybutyrate-co-hydroxyvalerate)
and natural polymers are collagen, albumin, chitosan, gluten, hyaluronate, cellulose, alginate,
starch. These biodegradable polymers are non-toxic and completely eliminated and degradation
occurs by oxidation enzymatic reaction or hydrolysis. So that it is very safe and effective vehicle
for delivering drug nanoparticles by increasing the degradation rate of polymeric matrix for
targeted delivery of drugs within the effected cells.
Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO319

DNA Fingerprinting Ocimum basilicum and O. sanctum through
RAPD Markers
Maryam Sarwat*
Pharmaceutical Biotechnology, Amity Institute of Pharmacy, Noida, UP – 201303
E mail: maryam21_7@yahoo.com; msarwat@amity.edu

India is a hotspot of biodiversity and is home to a wide variety of medicinally and economically
important plants. A number of techniques were being used from time immemorial for correct
identification of medicinal plants. Molecular markers being independent of any environmental
cues or developmental stage are proved to be very helpful in distinguishing valuable medicinal
plants from their adulterants. Ocimum from Lamiaceae is considered as queen of herbs. Most of
its species are used in traditional medicinal system against cough, cold flu, head and ear
infection, arthritis, rheumatism, malaria, fever, allergies and various skin diseases.
Leaf samples were collected from Lucknow, Delhi, NOIDA, Jaipur and Jammu. We have tested
20 RAPD primers, out of them 12 primers were selected for fingerprinting studies. The reaction
was carried out according to Sarwat et al. (2008). The amplification products were scored for the
presence (1) and absence (0) of bands across the genotypes to generate a binary matrix. The
binary matrix was analyzed using the NTSYS-pc to calculate the similarity values and generate
the phenogram. Jaccard’s similarity coefficient was utilized for estimating the pairwise similarity
.
Ocimum basilicum was found to be closer to Ocimum sanctum. Interspecific study of Ocimum
sanctum further revealed that although, the tulsi samples were taken from different places they
were morphologically and genetically similar depicting that there was not much genetic
diversity.From these plants, SCAR markers can be developed in future. They are highly efficient,
produce sharp and reproducible bands, are economical than other molecular techniques.
Key Words: Molecular Markers, Authentication, Herbal Drugs, AFLP, RAPD, SCAR

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO320
THIAZOLIDINE-2, 4-DIONE & ITS ANALOGUES AS ANTICANCER MEDIATED BY
DEPLETION OF INTRACELLULAR CALCIUM: A REVIEW
Karuna S. Shukla*1, Monika1, Shailendra Pandey2, Pooja Chawla3
1

School of Pharmacy, Babu Banarasi Das University, Lucknow
2

3

Research Lab KAPL Vasai (E) Mumbai

Gyani Inder Singh Institute of Professional Studies, Dehradun
Karunaforyou06@gmail.com

Calcium ions plays a crucial role in key biological processes. The major intracellular calcium
store in the endoplasmic reticulum(ER). Depletion of endoplasmic reticulum calcium stores
promote endoplasmic reticulum stress and that leads to the phosphorylation of eukaryotic
initiation factor 2 (eIF2) and thereby inhibition of translation initiation. Translation initiation
plays an important role in regulation of cell proliferation and malignant transformation and is
therefore an attractive target for the development of mechanism specific anticancer drugs.
Thiazolidine-2, 4-dione & its analogues exhibit anticancer properties mediated by inhibition of
translation initiation. These compounds causes partial & sustained depletion of intracellular
calcium stores because they simultaneously release calcium stores. This partial depletion of
intracellular calcium stores causes activation of eIF2 kinases (PKR or/and PERK) leading to the
phosphorylation of the α-subunit of eIF2 (eIF2 α) and inhibition of translation initiation and this
leads to the regulation of cell proliferation i.e. anticancer so it is concluded that thiazolidine-2, 4dione & its analogues as Anticancer mediated by depletion of intracellular Calcium.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO321
SIMULTANEOUS ESTIMATION OF PIOGLITAZONE AND LOVASTATIN BY RPHPLC
Monika1*, Karuna Shanker Shukla1, Shailendra Pandey2, Pooja Chawla3
1

School of Pharmacy, Babu Banarasi Das University, Lucknow
2
Research Lab. KALP Vasai (E) Mumbai.
3
Gyani Inder Singh Institute of Professional Studies, Dehradun
Email id: monika.mpharm@gmail.com
Diabetes is a chronic metabolic disorder that prevents the body from utilizing glucose completely
or partially. Obesity is major problem in diabetic patients. Being overweight means increased
insulin resistance. Therefore, Statins are now commonly prescribed for diabetic patients.
Pioglitazone is a thiazolidinedione derivative and it is used in patient with NIDDM. The
probable mechanism of action of Pioglitazone is through the selectively stimulation of nuclear
receptor peroxisome proliferator-activated receptor gamma (PPAR-γ). Whereas, Lovastatin is an
inhibitor of 3-hydroxy-3-methylglutaryl-coenzymeA reductase, an enzyme that catalyzes the
conversion of HMG-CoA to mevalonate. These drugs are therapeutically used to overcome
serious risk factors associated with cardiovascular diseases. A new simple and precise reverse
phase isocratic high performance liquid chromatography method has been developed for the
determination of Pioglitazone and Lovastatin in formulations. The separation was achieved on
millennium M Sil C18 column using acetonitrile: 20 mM NaH2PO4 (60:40 % v/v) as a mobile
phase and pH was adjusted 3.0 with 5% ortho phosphoric acid. The flow rate was kept at 1
ml/minute, the analytes were screened using UV detector at 230 nm wavelength. The retention
time of Pioglitazone and Lovastatin were found to be 4.1 and 6.5 minutes respectively.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO322
DOUBLE PYLORUS: AN UNCOMMON FEATURE OF STOMACH
Mayank Kulshreshtha*, Manjul Pratap Singh
School of Pharmacy, Babu Banarasi Das University, Lucknow.
Double pylorus (DP) is an uncommon condition consisting of a double communication between
the stomach and the duodenum. DP can occur as either a congenital abnormality or an acquired
complication of peptic ulcer disease. It occurs more often in men (2:1), as well as with other
peptic diseases. The majority of reported cases of double pylorus is acquired and are attributed to
complications of ulcers at the antrum-pyloric area or at the duodenal bulb. Most of them are
consequences of gastric ulcer, and only few cases are due to duodenal ulcer. In this patient, the
previous history of gastric ulcer and the presence of scar tissue at the endoscopic examination
indicated that the lesion are acquired. In diabetes the lack of microcirculation can be the cause.
The majority of the patients respond well to medical treatment, regardless of whether the fistula
is open or closed. However, refractory symptoms can occur in about 20% of the patients, and
surgical treatment is necessary. With the use of potent inhibitors of acid production such as the
proton pump inhibitors, we believe that this number may be reduced. In the clinical setting,
double pylorus can present itself with epigastric pain, dyspeptic symptoms and upper
gastrointestinal bleeding or can be found incidentally.
Key Words: Double pylorus, Duodenal ulcer, Proton pump inhibitors

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO323
TRITERPENOIDS: A POTENT HEPATOPROTECTIVE AGENT
1

Gunja Srivastava*, 1Manjul Pratap Singh, 2Anurag Mishra

1

2

School of Pharmacy, Babu Banarasi Das University, Lucknow.
Faculty of Pharmacy, Ashoka Institute of technology and Management, Varanasi.

Liver diseases are a major problem of worldwide proportions, people of nearly every age are
suffering from some or other problem related to liver. Liver damage is very common since
liver has to encounter several toxic substances during their metabolism. To counter with this
loophole there is a demand of such agents that has the capability to fight against such effects.
Due Growing interest in the elucidation of the biological functions of triterpenoids which are
ubiquitously distributed throughout the plant kingdom, they are major component of some
traditional medicinal herbs and traditionally being acclaimed as very potent hepatoprotective
agent. Plants with such constituents have been the remedy of choice for several ethnic
societies that has been subjected to hepatic ailments some or other times. In recent years it
has attracted considerable attention due to its double edged sword effect on liver defined in
terms of prevention and cures both. This review summarizes the beneficial effects of
triterpenoids and is an initiative to establish its identity as effective hepatoprotective agents
and also to explore this moiety to fullest of its extent in the field of research and development
so as to deplete this disease from the society and can be established as a boon for the
medicine industries those generating such hepatoprotective drugs.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO324
CURCUMIN AS A DRUG IN TREATMENT OF ORAL CANCER
Shaista Suhail1*, Kumud Nigam1, Saurabh Srivastava2
1

Department of Oral Pathology & Microbiology, King George’s Medical University, Lucknow,
UP, India

2

Department of Oral & Maxillofacial Surgery, King George’s Medical University, Lucknow, UP,
India

Curcumin (diferuloyl methane), a hydrophobic polyphenol derived from the dietary spice
turmeric. It possesses properties of anti-inflammatory and anti-cancer following oral
administration. It has mechanisms of action including several cell signalling inhibition pathways,
cell cycle (cyclin D1 and cyclin E), apoptosis (activation of caspases and down-regulation of
antiapoptotic gene products), proliferation (HER-2, EGFR, and AP-1), survival (PI3K/AKT
pathway), invasion (MMP-9 and adhesion molecules), angiogenesis (VEGF), metastasis (CXCR4) and inflammation (NF-jB, TNF, IL-6, IL-1, COX-2, and 5-LOX), effects on cellular enzymes
such as cyclooxygenase and glutathione S-transferases, 37mmune-modulation. Curcumin also
affects gene transcription mechanism and induce apoptosis in preclinical models. The activity of
curcumin reported against leukemia and lymphoma, gastrointestinal cancers, genitourinary
cancers, breast cancer, ovarian cancer, head and neck squamous cell carcinoma, lung cancer,
melanoma, neurological cancers, oral cancer and sarcoma reflects its ability to affect multiple
targets. Epidemiological and clinical studies have suggested that cancer could be prevented or
significantly reduced by treatment with anti-oxidant and anti-inflammatory drugs, therefore,
curcumin, a principal component of turmeric (a curry spice) showing strong anti-oxidant and
anti-inflammatory activities, might be a potential candidate for the prevention and/or treatment
of oral cancer. Phenolic compound (curcumin) also play an important role as chemopreventive
agents and the development of chemopreventive strategies is an urgent priority in public health.
Cancer is one of life threatening disease of the world. Herb is considered one of the greatest
man-made medicine(drug) which can be easily available and effective. It is the cheapest method
to cure the disease or to prevent it. The herb can be use to make gels, ointments, in tooth paste
etc. for prevention of disease in future. As many technologies are available now a days, it will be
great to make use of this herbs in many ways for prevention or cure of cancer.
Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO325
FORMULATION AND EVALUATION OF METOPROLOL TARTRATE
MICROSPHERES
M.V. Ramana*1, Kamal Dua2
1

2

Amity University, Lucknow, U.P., India
School of Pharmacy and Biomedical Sciences, The University of Newcastle, Newcastle,
Callaghan NSW 2308, Australia
mvramana@lko.amity.edu

Antihypertensive drugs were currently available in the market largely in the form of
conventional dosage forms. There is a need to develop controlled release drug delivery systems
for these categories, so as to optimize the therapy and accrue the benefits enumerated with such
drug delivery systems. One such approach is using polymeric microspheres as drug carriers.
Metoprolol tartrate is rapidly absorbed from both gastric and intestinal regions, after oral
administration.In order to retard the release rate of the metoprolol tartrate, microspheres were
prepared with varying concentrations of a mixture of a water insoluble polymer, ethylcellulose
(EC) and a water-soluble polymer, polyethyleneglycol-6000 (PEG-6000); Table 1. The prepared
microspheres were evaluated for various physicochemical characteristics and in-vitro drug
release.The percent yield of microspheres was in the range 75.2 to 87.3%. A considerable
amount of materials were entrapped into the microspheres (91.0%- 95.7%). This is quite
common with the solvent evaporation method. Particle size of microspheres ranged from 3.27
m to 163.5 m and the average diameter was found to be in the range of 73.2 m to 85.5 m.
FT-IR spectral analysis and DSC concluded the absence of any interaction between the drug and
carriers. The release-time profile of metoprolol tartrate from microspheres in 0.1 N hydrochloric
acid solution was to the extent of 33.4% to 60.2%. The release of metoprolol tartrate from MPT3 and MPT-4, in phosphate buffer solution (pH 7.4) was complete within 8 and 7 hours
respectively, whereas incomplete release (72.3%) occurred from MPT-1. Nearly complete
release (98.5%) of metoprolol occurred from MPT-2 in 10 hours, Hence, formulation MPT-2
would be a preferred formulation. The release of metoprolol followed the zero-order kinetics, as
the R2 value was higher for zero-order (R2 = 0.9642) than for the first-order (R2 = 0.8260) and
the release mechanism involved is diffusion rate limited (R2 = 0.9865) from microspheres. The
prepared microspheres of metoprolol tartarte eliminate the need for multiple dosing there by
Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

increasing patient compliance and decreasing the occurrence of adverse effects by providing a
prolonged release of drug.
PO326
Model-based Drug Development: Applicable to Oncology
Shubham Sharma*, Nimisha Mishra
ASET, Amity University Uttar Pradesh, Lucknow Campus, Lucknow
Model-based drug development (MBDD) is an approach that is used to organize the vast and
complex data streams that feed the drug development pipelines of small molecule and
biotechnology sponsors. Such data streams are ultimately reviewed by the global regulatory
community as evidence of a drug’s potential to treat and/or harm patients. Some of this
information is captured in the scientific literature and prescribing compendiums forming the
basis of how new and existing agents will ultimately be administered and further evaluated in the
broader patient community. As this data stream evolves, the details of data qualification, the
assumptions and/or critical decisions based on these data are lost under conventional drug
development paradigms. MBDD relies on the construction of quantitative relationships to
connect data from discrete experiments conducted along the drug development pathway. These
relationships are then used to ask questions relevant at critical development stages, hopefully,
with the understanding that the various scenarios explored represent a path to optimal decision
making. As MBDD becomes more integrated into the pharmaceutical research community, a
more rational explanation for decisions regarding the development of new oncology agents as
well as the proposed treatment regimens that incorporate both new and existing agents can be
expected. Hopefully, the end result is a more focussed clinical development programme, which
ultimately provides a means to optimize individual patient care.
Keywords: clinical pharmacology, model-based drug development, pharmacodynamics,
pharmacokinetics.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO327
EFFECT OF BUXUS WALLICHIANA BAILL EXTRACT AGAINST EXPERIMENTAL
INDUCED AMNESIA IN SWISS ALBINO MICE
Satyendra K Rajput*, Varshala Pal, Arun K Sharma, Shyam Sundar Agrawal
Department of Pharmacology, Amity Institute of Pharmacy, Amity University, Uttar Pradesh,
Sector - 125, Noida, 201303, India.
E-mail: skrajput95@amity.edu
The present study was undertaken to investigate the effect of Buxus wallichiana Baill (wood), on
scopolamine-induced amnesia in mice. Amnesia is the bench mark of Alzheimer’s diseases
(AD) which would become life threatening at sometime. Male mice were randomized to receive
different dose (125, 250, and 500 mg/kg, p.o) of methanol extract of Buxus wallichiana (MEBW)
for fifteen day against donepezil as reference standard. The Effect on learning and memory was
done using standard memory test includes elevated plus maze (EPM), morris water maze
(MWM), passive avoidance task (PAT). MEBW (125, 250 and 500 mg/kg, p.o) has shown
substantial lessen in transfer latency (TL) in EPM whereas, a significant increase has observed in
step down latency in PAT as compared to scopolamine (1 mg/kg, i.p) treated group. Moreover,
MWM test reveal the mark reduction in escape latency time during training by employing
MEBW (125, 250, 500 mg/kg, p.o). In Probe trial, these animals had spent more time compared
to scopolamine treated group. However these drugs did not alter locomotor activity. It has been
concluded that beneficial effect of MEBW on mice may be attributed to the facilitation of
cholinergic transmission and thereby improvement in memory. Therefore, it would be
worthwhile to explore the therapeutic potential of Buxus wallichiana in the management of
patients with cognitive disorders.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO328
Formulation development & Characterization of Ethosomal gel Approved for the
Treatment of Arthritis
Pratibha Gupta*, Anupum Kumar Sachan
DYANAND DINANATH COLLEGE OF PHRMACY NH# 86, HAMIPUR ROAD, KANPUR20914
e.mail-pguptabpharma@gmail.com
The present research has been under taken with the aim to develop topical ethosomal gel of
diclofenac by using such as carbopol ,propylene glycol, phospholipid, and soya phosphatidly
choline in different concentration .The oral use of diclofenac is not much recommended as it has
many side effect, thus this gel formulation is made for better patient compliance and reduced the
dose of drug and avoid the side effect like liver& a kidney damage. The different preformulation
studies i.e. Infrared and organoleptic study conforms its purity high moleculer weight ,water
soluble polymer of soya phosphatidly choline ,carbopol 940,phospholipid that possess very high
viscosity ,transparence film forming property at low concentration, gel formulation were
characterized for pH determination ,spreadability & drug release ,drug content ,viscosity
measurement and in vitro drug release .From the study, it was concluded the diclofenac gel
containing carbopol 940 showed good consistencey ,homogencity , spredability & drug release.it
given as wider prospect for the topical preparation .The gel preparation shows excellent
percutaneouse absorption of diclofenac and good characterization.
Key woards: diclofenac, carbopol 940, propylene glycol, drug release.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO329

Nano Particles in Drugs
Mridul Rajeev, Nikhar Shukla, Monika Kamboj, Richa Khare
Amity School of Applied Sciences, Amity University, Lucknow.
Nano particles are very effective in comparison to other one because they are small in size and
can be used anywhere for drug delivery. The main objective of nanoparticles is to control and
manipulate biomacromolecular constructs and supramolecular assemblies that are critical to
living cells in order to improve the quality of human health. By definition, these constructs and
assemblies are nanoscale and include entities such as drugs, proteins, viruses, cellular lipid
bilayers, cellular receptor sites and antibody variable regions critical for immunology and are
involved in events of nanoscale proportions. Therefore

nanotherapeutics is the only method

which will allow a deeper understanding of human longevity and human ills that include cancer,
cardiovascular disease and genetic disorders. This is the only technique which provides a wide
range of manmade nanostructures that may be controlled as a function of size, shape and surface
chemistry.

Key words: Biomacromolecular, supramolecular, nanotherapeutics and nanostructures

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO330
Role of Biotechnology in Pharmaceuticals
Nikhar Shukla*, Mridul Rajeev, Smriti Khare and Richa Khare
Amity School of Applied Sciences, Amity University, Lucknow
For hundreds of years humankind has used biotechnology in agriculture, food production,
and medicine. Depending on the tools and applications it often overlaps with the fields
of bioengineering, biomedical engineering, bio manufacturing, etc. In recent years, the number
of drugs of biotechnological origin available for many different diseases has increased
exponentially, including different types of cancer, infectious diseases, cardiovascular,
neurological, respiratory and autoimmune diseases. The pharmaceutical industry has used
different technologies to obtain new and promising active ingredients as exemplified by the
fermentation technique, recombinant DNA technique and the hybridoma technique. The
pharmaceutical industry in their attempts to discover new molecules has found annually in the
biotechnology industry with exponential growths. In this paper we reviewed the most important
biopharmaceuticals such as blood factors, hormones, enzymes and vaccines.
Key words: bioengineering, biomedical engineering, bio-manufacturing, Biopharmaceuticals
and hybridoma technique.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO331
Impact of chitosan nanoparticle in drug delivery
Upasana Yadav*
Amity School of Applied Sciences, Amity University, Lucknow-226017, U.P., India
E. Mail:upa_biotech@yahoo.com
The application of nanotechnology for the treatment, diagnosis, monitoring, and control of
biological systems has recently been determined by the National of Health (NIH) as
nanomedicine. The strategy of Nanoparticle delivery plays a significant impact on global
Pharmaceutical planning and marketing. Polymeric nanoparticles are used to control the drug
release, to improve the dissolution of poorly soluble drugs in addition to improve the
bioavailability of degradable substances such as protein. They also enhance the uptake of
hydrophilic substances across the epithelial layers and have the potential for intracellular drug
delivery. The submicron size range of nanoparticles is not only suitable for parenteral application
but also applicable for mucosal routes of administration, i.e., oral, nasal, and ocular mucosa
which are non-invasive route. Thus nanoparticle formulations are more advantageous over
traditional dosage forms. The main aim of the present review deals with the nanoparticles of
chitosan, which is a natural and bio-degradable polymer. The review focuses on the isolation,
purification, characteristic features derivatives of chitosan, preparation techniques, evaluation
methods and applications of chitosan nanoparticles.
Keywords: chitosan, nanoparticles, bioavailability, biodegradable polymer.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO332
CHEMICAL CONSTITUENTS AND PARTS OF PLANTS AS
IMMUNOMODULATORS
Kalpana Sonwani*, Ashutosh Kumar Yadav
Department of Pharmacology , School of Pharmacy , BBD University , Lucknow
drkalpanapharmacology@gmail.com
Immunomodulators (IM) are the drugs affecting the immune system. They are used when the
immune response is impaired. Hence to maintain a Disease Free State, modulation of immune
response by either its stimulation or suppression, can be a helpful therapy . Immunodeficiencies
occurs when one or more of the components the immune system are inactive. It included
autoimmunity, hypersensitivity and HIV etc. IM include corticosteroids, cytotoxic agents,
thymosin and immunoglobulins etc. The present review is focused Immunomodulatory effects
of some medicinal plants based on the chemical constituents such as Flavanoids , Alkaloids ,
Phenolic compound , Steroids ,Terpenoids and Tannins present in that particular plant such as
Ficus carica (leaves) ,Aloe vera (Leaves), Cleome gynandra (Aerial parts), Allium sativum
(Whole plant) , Asparagus racemosus ( Root), Tinospora cordifolia (Stem), Ocimum sanctum
(Whole plant) ,Curcuma longa (Balb), Mangifera indica (Stem bark) etc. had been discussed
which are

previously explored by the various researchers for their immunomodulatory

activity. As discussed above, it can be conclude that there is a wide scope in herbal drugs and
their formulations in the treatment of immune disorders.
Keywords: Immunomodulators, HIV, Immunodeficiency

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO333
“CLINICAL BENEFITS OFHERBAL BASED ADAPTOGENS ”
Pooja Rao*, Dharamveer
Department of Pharmacology, School of Pharmacy, BBD University, Lucknow
Email id:-poojaraoaryan 155@gmail.com
Plant adaptogens are compounds that increase the ability of an organism to adapt to
environmental factorsand to avoid damage from such factors. The beneficial effects of multi dose
administration of adaptogens are mainly associated with the hypothalamic–pituitary adrenal
(HPA) axis.Thesingle dose application of adaptogensis important in situations that require a
rapid response to tension or to a stressful situation.In this case, the effects of the adaptogens are
associated with another part of the stresssystem, namely, the sympatho–adrenal-system (SAS),
that provides a rapid response mechanism mainly to control the acute reaction of the organism to
a stressor.Theadaptogens like Rhodiolarosea, Schizandrachinensis, Eleutherococcussenticosus
etc showed stimulating effects.The phenolic compounds include phenylpropanoids and
phenylethane derivatives such as salidroside (rhodioloside), rosavin, and lignans are structurally
similar to the catecholamines—the mediators of the sympatho-adrenal- system (SAS)Recent
pharmacological studies of some adaptogens give a rationale to their effects at the molecular
level. Adaptogens may be regarded as a novel pharmacological category of anti-fatigue agents
that perform the several functions including induce increased attention and endurance in
situations of decreased performance caused by fatigue and/or sensation of weakness and reduce
stress-induced impairments and disorders related to the function of stress (neuro-endocrine and
immune) systems.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO334
ANIMAL MODELS FOR THE STUDY OF STRESS VULNERABILITY RESILIENCE
Richa Mishra*, Ashutosh Kumar Yadav
Department of Pharmacology, School of Pharmacy, BBDUniversity, Lucknow
Richamishraa7@gmail.com
Resilience is defined as the adaptive maintenance of normal physiology, development and
behaviour in the face of pronounced stress and adversity. Resilience to stress has been
documented and characterized in animal models throughout the lifespan. The different models
are Early Life stress, Chronic unpredictable stress, Chronic social defeat stress, and Learned
helplessness.
Early Life stress- animal models of early life stress typically involve exposure to stressful stimuli
during either the prenatal or post natal periods. Chronic unpredictable stress -In CUS paradigms,
animals are exposed to varying mild stressors sequentially for a period of 1e7 weeks. Chronic
social defeat stress- Chronic social defeat stress (CSDS) is a prolonged social stress paradigm
used to delineate stress resilient and susceptible phenotypes in adult rodents. Learned
helplessness-The learned helplessness (LH) model is an acute stress paradigm that, similar to
CSDS, produces heterogeneous responses.Modern research on stress related disorders has
yielded numerous potential targets and biomarkers for diagnosis and treatment, largely due to an
enhanced focus on alternatives to monoamine-based mechanisms, such as epigenetic
mechanisms, immune-related factors, sex, and the biology of resilience.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO335
NATURAL MEMORY BOOSTERS IN INDIAN HERBAL PLANTS
Jyoti Gupta*, Mayank Kulshreshta
Department of Pharmacology, School of Pharmacy, BBD University, Lucknow
Memory is the usual consequence of learning and reflects the enduring changes in the nervous
system that result from transient experiences. Memory impairment is the hallmark symptom of.
It is estimated that 35.6 million people worldwide and 5.5 million peoples in India.
The Indian Medicinal System of Ayurveda has a treasury of such memory enhancing drugs like
Bacopa monnieri, Withania somnifera , Glycyrrhiza glabra , Celastrus paniculata , Centella
asiatica ,Convolvulus pluricaulis ,Zingiber officinal.
Bacopa monnieri is a nervine tonic famous for its memory and attention enhancement functions,
Withania somnifera

shows that it stimulate the growth of axons and dendrites in human

neuroblastoma cells, Celastrus paniculata is an folk medicine, the seeds are boiled and taken for
blood purification, Centella asiatica has been found to improve the power of concentration,
Glycyrrhiza glabra which improves memory and intellect, Convolvulus pluricaulis It relieves
mental stress, controls blood pressure and also improves mind's ability.
Herbs play a promising role in the early treatment of poor memory including Alzheimer’s and
dementia.. Memory enhancer herbs enhance the memory and increase blood circulation in the
brain.
Keywords: Memory impairment, Alzheimer’s disease, Dementia, Herbal medicines.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO336
CHRONIC UNPREDICTABLE STRESS:A CLASSICAL MODEL OF DEPRESSION
Garima Singh*, Dharamveer
Department of Pharmacology, School of Pharmacy, BBD University , Lucknow
Garima19191singh@gmail.com
Chronic unpredictable stress (CUS) induce depression is generally thought to be the most
promising and valuable depressive model. In CUS paradigms, animals are exposed to varying
mild stressors sequentially for a period of 1e7weeks.Stressors can include mild foot shock,
physical restraint, tail suspension, light/dark cycle disruption, food or water restriction, changes
to cage mate, etc., and are changed after several hours to minimize habituation.CUS produces a
range of depression and anxiety-like behaviors in rodents including anhedonia, measured as
decreased sucrose preference, despair-like behavior, measured as increased immobility in the
forced swimand tail suspension tests, and novelty suppressed feeding,measured as a decrease in
approach to a novel food item. Mice exposed to CUS also display decreased grooming,
aggression, and sexual behaviors. Certain CUS-induced behavioral changes, such as novelty
suppressed feeding, can be reversed only by chronic antidepressant treatment making CUS
relevant to human antidepressant responses. Female mice display immobility in the forced swim
test after just 6 days of subchronic unpredictable stress (SCUS) whereas males are generally
resilient to SCUS and require 20e 28 days of CUS exposure to elicit depression- and anxiety-like
behavior. Sex differences and age effects in susceptibility to CUS-induced depression and
anxiety-like behavior make this a powerful tool for investigating the hormonal and neural basis
for stress induced depression.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO337
FLAVONOIDS AS AN ANALGESIC, ANTIPYRETIC AND ANTI-INFLAMMATORY
AGENT
Sarasvatee kumari*, Ashutosh Kumar Yadav
Department of Pharmacology, School of Pharmacy, BBD University, Lucknow
Pain is defined as an unpleasant sensory and emotional experience associated with actual or
potential tissue damage, while inflammation is defined as the local response of living
mammalian tissues to injury due to any agent. It is also a body defence reaction in order to
eliminate or limit the spread of injurious and tissue and it is manifestation of the body response
to tissue damage and infection. Flavonisds are a group of plant metabolites thought to provide
health benefits, cell signalling pathways and antioxidant effect. Different types of flavonoids,
flavonols and

flavanones, flavan-3-ols anthocyanins, flavones and Isoflavones. Flavonoids

MOA a number of flavonoids such as hesperidin, apigenin, luteolin, and quercetin are reported to
possess anti-inflammatory and analgesic effects it’s may affect specifically the function of
enzyme systems especially tyrosine and serine-threonine protein kinases. The inhibition of
kinases is due to the competitive binding of flavonoids with ATP at catalytic sites on the
enzymes. Flavonoids also inhibit phosphodiesterases and biosynthesis of protein cytokines that
mediate adhesion of circulating leukocytes to sites of injury flavonoids are potent inhibitors of
the production of prostaglandins. As conclusion, it can be concluded that there is wide scope of
herbal drugs and their formulations used for analgesic and anti-inflammatory disorder.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO338
ROLE OF β-AMYLOID PROTEIN IN ALZHEIMER DISEASE
Khushaboo Singh*, Dharamveer, Mayank Kulshreshtha
Department of Pharmacology, School of Pharmacy, BBD University, Lucknow
Khushaboo.singh.8892@gmail.com
Alzheimer’s disease (AD) is a progressive neurodegenerative disorder characterized by severe
cognitive impairments. A major histopathological hallmark of AD is the presence of amyloid
deposits in the parenchyma of the amygdala, hippocampus, and neocortex. β-amyloid is a small
piece of a larger protein called “amyloid precursor protein” (APP). The main component of
amyloid is the β-Amyloid protein (Aβ), a 39.43 amino acid peptide composed of a portion of the
transmembrane domain and the extracellular domain of the APP. Aβ deposition leads to synaptic
degeneration and interacts with different types of central nervous system receptors; hence, it
disrupts neuronal homeostasis. Moreover, Aβ deposition along the cerebral vessels alters their
tonicity and triggers some of the cerebrovascular deficits. Furthermore, its accumulation disrupts
intracellular Ca2+ homeostasis which ultimately reduces neuronal Ca2+ buffering capacity and
increases excitotoxicity outcomes. Also, Aβ peptides may fold in different ways and show a
prion-like pathology in the brain of AD patients.
Keywords: Alzheimer’s disease , β-Amyloid protein, Amyloid precursor protein.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO339
A REVIEW ON EFFECT OF ANXIETY AND STRESS ON MEMORY
Deepa Shukla*1,2, Sajal Srivastava2, Talha Jawaid
1
2

Rajkumar Goel Institute of Technology, Ghaziabad

Amity Institute of Pharmacy, AUUP, Lucknow Campus

Experiencing anxiety, fear and stress is considered as a normal part of life but when these acute
emotional reactions become more frequent or chronic, they can significantly interfere with daily
activities.
Chronic stress is a pathological state that is caused by prolonged activation of the normal acute
physiological stress response, which can wreak havoc on immune, metabolic and cardiovascular
system and leads to degeneration of the hippocampus part of brain.
There is an extensive overlap of the brains neural activity in anxiety, fear and stress which may
explain the link between chronic stress and the development of neuropsychiatric disorders,
including depression and Alzheimer’s disease. Pathological stress, anxiety and chronic stress are
associated with structural degeneration and impaired functioning of the hippocampus which may
account for the increased risk of developing neuropsychiatric disorders including depression and
dementia.
My review suggests that an anxiety and stress play an important role in dementia. Review also
suggests that stress induced damage to the hippocampus is not completely irreversible.
Key Words: Anxiety, stress, dementia and Hippocampus

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO340
EVALUTION OF HEPATOPROTECTIVE POTENTAIL OF ALCOHOLIC AND
AQUEOUS EXTRACT OF TERMINALIA BELERICA IN RATS
Sachin Neekhra*, Rohit Verma
College of pharmacy, SR Group of institution, Ambabai, Jhansi U.P.
E mail ID- Sachinpharma13@gmail.com
To evaluate the hepatoprotective activity of alcoholic (ALTB) and aqueous (AQTB) extract of
fruit of Terminalia belerica by acute and chronic hepatotoxic models. Acute liver damage was
produced by CCl4 and paracetamol, while chronic liver damage was induced by alcohol. Physical
parameter (liver weight and wet liver volume), biochemical parameters (serum AST, ALT, ALP,
albumin, total protein, direct and total bilirubin levels) and histology of liver were performed to
assess the hepatoprotective activity. CCl4, paracetamol and alcohol administration resulted in
significant elevation of rat liver weight and liver volume, serum AST, ALT, ALP, direct bilirubin
and total bilirubin levels, while albumin and total protein were found to be decreased compared
to normal group. Pretreatment of with silymarin, ALTB and AQTB significantly prevented the
physical and biochemical changes induced by these hepatotoxins. Histopathology of liver
confirmed our finding as the treatment with the extracts resulted in minor liver cell damage
compared to control (toxic) group. The hepatoprotective effect offered by ALTB (400 mg/kg,
p.o.) was found to be significantly greater than AQTB (400 mg/kg, p.o.) and standard (silymarin
50 mg/kg, p.o.) group.
Keywords: Terminalia belerica, Biochemical parameters, Hepatoprotective, Silymarin, CCl4,
Paracetamol, Alcohol

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO341
Mechanistic approaches of different models of Alzheimer’s disease
Dan Bahadur Rokaya*, Himani Awasthi
Amity University Uttar Pradesh, Lucknow Campus, U.P., India
Alzhiemer's disease (AD) is the neurodegenarative disorder, one of the most common causes of
dementia in elderly people. It starts slowly and gets worse over time. The most common early
symptom is difficulty in remembering recent event (short term memory loss), the cause of AD is
poorly understood. As the disease advances symptoms can include problem with language,
disorientation, mood swings,

Loss of motivation and behavioral issue. The AD brain is

characterized by two abnormal structure; one, extracellularly Amyloid beta (Aβ) plague and
second, intracelluarly Neurofibrillary tangle (NFT). Different models are proposed to induce AD
in-vivo.

Cholinergic

hypothesis,

chronic

neuroinflammation,

neurotoxicity,

hyperphosphorylation of tau- protein are main target for AD model. Neuroinflammation,
neurotoxicity is mainly due to the oxidative stress, insulin resistance to brain.
Key words: AD, Aβ plague, NFT, oxidative stress, ICV, cognitive impairment.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO342
Metal–Organic-Framework Nanoparticle as a Potential for Drug Delivery and Imaging
Upasana Yadav*, Archna Tiwari
Department of Amity School of Applied Sciences, Amity University, Lucknow-226017, U.P.,
India,
upa_biotech@yahoo.com
In the domain of health, one important challenge is the efficient delivery of drugs in the body
using non-toxic nanocarriers. Most of the existing carrier materials show poor drug loading
(usually less than 5 wt% of the transported drug versus the carrier material) and/or rapid release
of the proportion of the drug that is simply adsorbed (or anchored) at the external surface of the
nanocarrier. In this context, porous hybrid solids, with the ability to tune their structures and
porosities for better drug interactions and high loadings, are well suited to serve as nanocarriers
for delivery and imaging applications. Here we show that specific non-toxic porous iron(III)based metal–organic frameworks with engineered cores and surfaces, as well as imaging
properties, function as superior nanocarriers for efficient controlled delivery of challenging
antitumoural and retroviral drugs (that is, busulfan, azidothymidine triphosphate, doxorubicin or
cidofovir) against cancer and AIDS. In addition to their high loadings, they also potentially
associate therapeutics and diagnostics, thus opening the way for theranostics, or personalized
patient treatments.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO343
WILDLY GROWING PLANTS AS A SOURCE OF AN ALTERNATE MEDICINE: A
POSSIBLE DRUG DEVELOPMENT STRATEGY
Chandni Tandon1*, Priti Mathur1 and Manodeep Sen2
1
2

Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow- 226028

Department of Microbiology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow226010
chandni.tndn@gmail.com

Plants are considered as a richest source of therapeutic products in many traditional cultures and
are also becoming popular in modern society. Moreover, due to the continuous development of
bacterial resistance to synthetic antibiotics, natural products are increasingly in demand because
of its greater availability and lesser side effects. Taking this into consideration, the ethanol and
aqueous extracts of Cannabis sativa and Datura inoxia were prepared to evaluate its inhibitory
effect using agar well diffusion assay against the clinical strains of Staphylococcus aureus,
Escherichia coli and Pseudomonas aeruginosa isolated from urine and pus. Ethanolic extract of
both plants were found effective against gram positive as well as gram negative strains, of which
strains of Staphylococcus aureus were found inhibited with greater zone of inhibition compared
to Escherichia coli and Pseudomonas aeruginosa. Crude ethanolic extract of the plants were
further fractioned through column chromatography. Each isolated fractions were again tested for
their antibacterial activity against the most susceptible strain i.e. Staphylococcus aureus. Our
finding suggested that the studied plants could be a potential source of a new class of broad
spectrum of antibiotics. Further research is in process to identify the compounds and its
mechanism of action responsible for antibacterial activity of the plants.
Keywords: Cannabis sativa, Datura inoxia, agar well diffusion assay, clinical strains, column
chromatography.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO344
JAPANESE ENCEPHALITIS
*Mona Pal
Institute of Pharmaceutical Sciences & Research (IPSR) sohramau, unnao.
Japanese encephalitis virus (JEV) causes Japanese encephalitis in Asia with around 50,000 cases
and 10,000 death per year in children below 15 years of age. The JEV has shown tendency to
extend to other geographic region case fatality average 30% and high percentage of survivors are
left with permanent neuropsychiatric sequelae. Currently there is no cure for JEV and treatment
is mainly supportive patient are not infectious but should avoid further mosquito bites. A number
of antiviral agent have been investigation however none of these have convincingly have been
shown to improve the outcome of JEV in this review the current knowledge of the epidemiology
and the pathogenesis of this deadly disease have been summarized.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO345
ANTIVIRAL & ANTIRETROVIRAL
Sanni Gangwar*
Institute of Pharmaceutical Science & Research, Unnao
Polymeric prodrug or polymer drug conjugate is an effective and fast growing technique for
improved use of drugs for therapeutic application. Polymer conjugates drugs generally exhibit
prolonged half-life, higher stability, water solubility, lower immunogenicity and antigenicity and
specific targeting to tissue or cells. Polymers are used as carriers in polymeric
prodrugs/macromolecular prodrugs for the delivery of drugs, proteins targeting moieties, and
imaging agents.
The polymeric prodrug can be regarded as drug delivery systems that exhibit
their therapeutic activities by means of releasing smaller therapeutic drug molecules from a
polymer chain molecule for a prolonged period of time which result in enhanced
pharmacokinetic behavior by increasing the t1/2, bioavilability, and hence prolonged
pharmacological action. The potential of the polymer drug conjugates have already been proved
by success of many products in the market for the treatment of different diseases.
A model for macromolecular prodrugs was first proposed by Sir Ringsdorf’s in the mid-1970s.
Keywords:- Polymeric Prodrug, Polymers, Enhanced Pharmacokinetics Behavior, Drugpolymer Conjugate, Ringsdorf’s model.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO346
Atomic level analysis of envelop protein from different strains of HIV virus
Parul Tripathi, Parul Johri, Aditi Singh*
Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow Campus, Malhaur,
Gomti Nagar Extension, Lucknow- 226028, India.
Email: asingh3@lko.amity.edu
Human immunodeficiency virus (HIV) a lentivirus, continues to be a major health problem
having claimed more than 34 million lives so far. The virus infects vital cells of human immune
system, the helper T cells (or CD4+ T cells), thus disturbing the CD4+/CD8+ T-cell ratio. With
antiretroviral therapy, people with HIV have increased life span, but there is an urgent need to
study mechanism of latent infection and reservoirs of infection. In the present work we tried to
analyze the envelop protein of 30 different strains of HIV. The gene ontology of HIV states that
the envelop protein is the main protein for the virus entry into the host cell. It fuses virus
membrane with host’s endosome membrane and plasma membrane. The sequences of envelop
proteins of HIV were retrieved from the proteome section of Uniprot database
(http://www.uniprot.org/). The sequences were parsed and subjected to a perl programming code
for calculation of carbon atom content in them. The range of carbon atom for envelop protein
was from 31.51% to 32.02%. This clearly indicates that nature tends to maintain a very specific
threshold of carbon atom for the envelope proteins. This benchmark could be further used for the
characterization of other virulent proteins.
Keywords – Carbon, envelop protein, HIV, perl programming.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO347
Proteome wide interaction analysis of mitochondrial proteins
Sarita Jha, Parul Johri*, Mala Trivedi
Amity Institute of Biotechnology, Amity University Uttar Pradesh,
Lucknow Campus, Malhaur, Gomti Nagar Extension, Lucknow- 226028 (UP)
pjohri@lko.amity.edu; +91 8765580920
Protein-protein interaction (PPI) represents a vital aspect for the functioning of protein, a key to
almost all biological processes. In order to accomplish their respective task, a protein relies on
interaction with other proteins. Comprehensive database of protein protein interaction have
provided a platform for both experimental and bioinformatics research in this area.
In the present work we have identified common mitochondrial protein amongst three model
organisms Homo sapiens, Sacchromyces cervisiae, and Caenorhabiditis elegans. These common
proteins were then subjected to pair wise alignment using ALIGN tool and the protein having
zero e-value was identified amongst them. This protein cytochrome c oxidase subunit-1 was
further subjected for its protein-protein interaction studies using string database.
The interacting partners were analyze and studied. Most of the interacting partners were from
cox gene family .But we also recognized other proteins like Y71H2AM.5.2, O9C5.2, and
F26E4.6, which does not belongs to cox gene family but were potential interacting partners of
our protein of interest.
Keywords: align tool, cox protein, PPI, String Database.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO348
MOLECULAR DOCKING OF RESVERATOL WITH HIGH THROUGHPUT
NEURODEVELOPMENT INJURY DATA UNLOCKS POTENTIAL DRUG TARGETS.
Ruchi Yadav,1 A.B.Pant2, Prachi Srivastava1*
1. AMITY Institute of Biotechnology, AMITY University Uttar Pradesh, Lucknow, UP, India
2. Invitro Toxicology Division, Indian Institute of Toxicology Research, Lucknow, India
Resveratrol is a natural phenol, and aphytoalexin produced naturally by several plants in
response to injury. There is exponential evidence since 1939 in the literature that Resveratrol is a
promising natural compound for prevention and treatment of a variety of human diseases.
Resveratrol is also reported to be effective against neuronal cell dysfunction and cell death,
Huntington's disease and Alzheimer's disease. Molecular studies shows that Resveratrol is
associated with an induction of genes for oxidative phosphorylation and mitochondrial
ubiogenesis, effect of Resveratrol is known to extend lifespan, impacts mitochondrial function
and metabolic homeostasis. In current work we have mapped effectiveness of Resveratrol against
injured neurodevelopment samples. In this study four samples were prepared (Control,
Resveratrol, MCP Pre Exposed to Resveratrol, MCP Post Exposed to Resveratrol). Datasets of
prepared samples were taken to investigate the neuroprotective role of Resveratrol against
exposure of monocrotophos. In silico expression analysis of different data sets is done by refined
protocol of R and Bioconductor .Differentially expressed gene analysis indicates variation in
expression of ATRX gene which was found to be significantly decreased in the presence of RV
exposure. ATRX gene( alpha thalassemia/mental retardation syndrome X-linked ) found to be
involved in developmental stage and reflects many specific anomalies viz. Disability in growth
retardation, neural tube defects , severe developmental delays, intellectual disability, anaemia
secondary to alpha-thalassemia and embryonic death. Further docking studies and interaction
analysis of Resveratrol against ATRX protein signifies Resveratrol as potential drug against
neurological development disorders.
Keywords: ATRX Gene, Drug Targets, Neurodevelopment, Resveratrol, R and d Bioconductor

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO349

EFFECT OF DESMODIUM GANGETICUM ON ETHYLENE GLYCOL
INDUCED NEPHROLITHIASIS IN RATS
Chetan Rastogi1*, Mohd. Muddassir Khan1, Shravan Paswan1,2, Preet Verma1,2, Alok Ranjan
Gaur1, Ch. V. Rao1, Sajal Srivastava2
1

Department of Ethnopharmacology & Gastropharmacology, CSIR-National Botanical Research
Institute, Lucknow
2

Amity Institute of Pharmacy, Amity University, Lucknow

The present study was carried out to evaluate the Anti-nephrolithiatic Activity of Desmodium
gangeticum on ethylene glycol induced nephrolithiasis in rats.Nephrolithiasis in male rats was
produced by inducing ethylene glycol 0.75% (v/v) with drinking water to give calcium oxalate
crystals. The hydroalcoholic Desmodium gangeticum plant extract (DGPE) orally at 200 and 400
mg/kg was administered along with ethylene glycol for 28 days. On 28 day, 24 h urine sample
was collected from individual rats and used for estimation of urine volume, pH and number of
calcium oxalate crystals. The serum sodium, potassium, phosphorus, magnesium, total calcium,
BUN, uric acid and creatinine were estimated in each animal. The plasma was used for the
estimation of MDA. The paraffin kidney sections were prepared to observe the histological
changes.
The lithiasis control had significant changes in levels of urine volume, pH and number of
calcium oxalate crystals, the significant increase was observed in serum sodium, phosphorus,
total calcium, BUN, uric acid and creatinine (stone promoters), decrease in potassium and
magnesium (stone inhibitors) levels and increase in plasma MDA level when compared to
normal. The paraffin kidney sections show significant histopathological changes. The treatment
of DGPE at 200 mg/kg and 400 mg/kg doses, significantly changed the levels of urine volume,
pH and number of calcium oxalate crystals, decreased the levels of liver function parameters,
stone promoters, and stone inhibitors and also in MDA levels when compared with lithiasis
control, in EG induced nephrolithiasis after 28 days.
The DGPE at the dose of 400 mg/kg, found to be more effective in decreasing nephrolithiasis,
regeneration of renal tissues and liver damage in male rats.
Keywords: Nephrolithiasis, Desmodium gangeticum, Ethylene glycol.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO350

EFFECT OF BERBERIS ARISTATA ON STREPTOZOTOCIN INDUCED
DIABETIC IN RATS
Mohd. Muddassir Khan1*, Chetan Rastogi1 , Preet Verma1,2, Shravan Paswan1,2, Alok Ranjan
Gaur1, Ch. V. Rao1, Sajal Srivastava2
1

Department of Ethnopharmacology & Gastropharmacology, CSIR-National Botanical Research
Institute, Lucknow
2

Amity Institute of Pharmacy, Amity University, Lucknow

Berberis aristata was used in treatment of inflammation, hypertension, kidney diseases and also
in diabetes according to ayurvedic formulary.The present study was carried out to evaluate the
Antidiabetic Activity of Berberis aristata on Streptozotocin induced diabetic in rats.
Streptozotocin was induced in male rats by adding Streptozotocin 60 mg/kg (w/v) in citrate
buffer 0.1M pH 4.5. The hydroalcoholic Berberis aristata ethanolic extract (BaEe) orally at 250
and 500 mg/kg was administered along with Streptozotocinfor 15 days. On 15 day, 24 h urine
sample was collected from individual rats and used for estimation of urine sugar (glycosuria).
The serum SGOT, SGPT, ALP, total bilirubin, BUN, Creatinine, lipid profile and antioxidant
levels was estimated in each animal. The paraffin pancreas sections were prepared to observe the
histological changes.
The disease control had significant changes in urine sugar, body weight, the significant increase
was observed in serum SGOT, SGPT, ALP, total bilirubin (liver function parameters), BUN, and
Creatinine, lipid profile levels and plasma MDA levels were increased when compared to
normal. The paraffin kidney sections show significant histopathological changes. The treatment
of BaEe at 250 mg/kg and 500 mg/kg doses significantly changed the levels of urine sugar but
decreased the levels of liver function parameters, lipid profile and also in antioxidant levels when
compared with diabetic control after 15 days in STZ induced. The BaEe at the dose of 500
mg/kg, found to be more effective in decreasing Diabetic, regeneration of renal tissues and liver
damage in male rats.
Keywords:Anti-diabetic, Berberis aristata, Streptozotocin, Antioxidant.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO351
HPTLC analysis for the quantification of gallic acid and quercetin in methanolic fraction
of Limonia acidissima L. fruits
Shikhar Verma1,2, Abhishek Gupta1, M.V Ramana2, AKS Rawat1
1

Pharmacognosy & Ethnopharmacology Division,
CSIR- National Botanical Research Institute, Lucknow-226001
Phone Number: 9616519322; E-mail: vermashikhar001@gmail.com
2
Institute of Pharmacy, Amity University, Lucknow

A high-performance thin layer chromatography (HPTLC) method for the simultaneous
quantitative determination of gallic acid and quercetin in methanolic fractions of Limonia
acidissima L. fruits was developed for the first time in this species. Methanol was found to be
best for highest possible recovery of target analytes. For achieving good separation, a mobile
phase of toluene: ethyl acetate: formic acid (5:4:1, v/v/v) was used. The densitometric
determination was carried out at 310 & 254nm in reflection/absorption mode. The calibration
curves were linear in the range of 100-600 ng per spot for gallic acid and quercetin.
The methanolic fractions of Limonia acidissima L. fruits showed the presence of gallic acid
(0.07%) and quercetin (0.14%). The proposed method is simple, precise, specific, accurate, less
time consuming and cost effective. The statistical analysis of data obtained proves that the
method is reproducible and selective and can be used for routine analysis of reported phenolic
compounds in crude drug and extracts. The simultaneous quantification of these compounds has
not yet been reported in Limonia acidissima L., which may be utilized for the proper
standardization of the drug.

Keywords: HPTLC, Limonia acidissima L., gallic acid, quercetin

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016
PO352

Natural Antioxidants
Ankan Rastogi, Musab Rafi, Himanshu Jaiswal
Amity Institute of Pharmacy, AUUP, Lucknow Campus

The constant exposure of the skin to oxidative stress results in damage to cellular DNA and cell
membrane lipids and proteins. To combat this problem, the skin contains a number of
antioxidants that protect against oxidative injury. However, these cutaneous antioxidants can be
depleted by sun exposure and environmental insults, resulting in an overload of oxidation
products. Thus, topical antioxidants that replenish the antioxidant capacity of the skin have the
potential to prevent oxidative damage. A number of natural antioxidant ingredients also have
anti-inflammatory properties, and can be used in the treatment of oxidative damage such as
photo-aging and perhaps even skin cancer. This article summarizes the active components,
pharmacologic properties, and clinical effectiveness of a number of natural antioxidant
ingredients including soy, mushroom extracts, teas, Coffee Arabica (Coffee Berry), Pinus
pinaster (Pycnogenol), and Poly-podium leucotomos. Recent clinical trials suggest that these
compounds have promising efficacy in the topical treatment of oxidative stress-induced
dermatoses.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO353
Glycosylation in Lipase enzyme of Homo sapiens and its correlation with
carbon composition
Parul Johri*, Shweta Sachan, Aditi singh
Amity Institute of Biotechnology, Amity University Uttar Pradesh,
Lucknow Campus, Malhaur, Gomti Nagar Extension, Lucknow- 226028 (UP)
* Corresponding Author: pjohri@lko.amity.edu; +91 8765580920
Post translational modifications (PTM) are the modifications made to the protein after or during
the protein synthesis. PTM occurs on the N or C terminal of protein or on the amino acid side
chains. These are important for cell signalling and functioning. PTM involves addition of
functional groups like isoprenoid, lipoate, acetyl group, alkyl group and many more. One
amongst all these is glycosylation, addition of a glycosyl group to protein. There are basically
two types of glycosylation – N-liked glycosylation and C-linked glycosylation. In the present
work we have studied the human lipase enzyme having glycosylation as the modification. All the
sequences of lipase enzyme were retrieved from the Uniprot database and were subjected to
atomic level sequence analysis. The carbon content of the sequence were calculated using a perl
script and was subjected to statistical analysis. The carbon content of the proteins having Nlinked glycosylation was ranging from 31.36% to 32.91%, where as the carbon content of the Olinked glycosylated proteins was 31.31% to 31.76%. This clearly demarks that nature tends to
have a threshold of carbon content for deciding the type of glycosylation in proteins.
Keywords – carbon, glycosylation, post translational modification.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO354
Yoga: Life of Science
Jyoti Wagh1, 2*, Sandeep1, 3, Ashutosh Mishra3 , Rahul Shukla4, Saikat Sarkar4, Sumit Gupta5
1. Research Scholar, Department of Pharmaceutical Science, Jayoti Vidyapeeth Women’s
University, Jaipur
2. MES college of pharmacy, Sonai, Ahmed Nagar, Maharashtra, India
3. A.N.D. College of Pharmacy, Babhnan, Gonda, Uttar Pradesh – 271 313
4. Amity Institute of Pharmacy, AUUP, Lucknow Campus, Uttar Pradesh.
5. RPIIT, Karnal, Haryan.

The word "yoga" derives from the Sanskrit root yuj ("to yoke") which means "to join" or "unite”.
Basically it is the "union of the individual atma (soul) with Paramatma (Supreme being), the
universal soul." This can be defined as union with the Divine by integration of body, mind, and
spirit. The people find out they are always in relaxed, calm, charming and attractive. Weight
loss, a strong and flexible body, glowing beautiful skin, peaceful mind, good health – whatever
you may be looking for, yoga has it on offer. However, very often, yoga is only partially
understood as being limited to asanas (yoga poses). As such, its benefits are only perceived to be
at the body level and we fail to realize the immense benefits yoga offers in uniting the body,
mind and breath. When you are in harmony, the journey through life is calmer, happier and more
fulfilling. As well as they were having fitness and power of finding solutions of difficult
problems. In current poster different position of yoga have been discussed.
Key word: Yoga, Atma, Union, Divine, Asanas.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO355
Preventive measurements to Stress in current environment
Ashutosh Mishra1*, Rahul Shukla2, Sandeep1
1.

A.N.D. College of Pharmacy, Babhnan, Gonda, Uttar Pradesh – 271 313

2. Amity Institute of Pharmayc, AUUP, Lucknow Campus, Uttar Pradesh.

The present world stress has become a major concern of the modern times as it can cause harm to
employee’s health and performance. Work related stress costs organization billions of dollars
each year through sickness, turnover and absenteeism. So it becomes necessary for every
organization to know the factor causing stress among the employees as well as how they cope up
with stress to make the employee more participative and productive. This study was conducted to
find out the factor causing stress and to know how they cope up with stress. The Research design
used was a descriptive research. The primary data has been collected through a questionnaire
method.
Keywords: Stress, Coping Strategies, Employee Health, Performance

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO356
A REVIEW ON NECROTIZING FASCIITIS-A LIFE THREATENING
INFECTION OF SKIN AND SOFT TISSUES
Ankita Tripathi, Suraj Neupane
Amity University Uttar Pradesh, Lucknow campus, U.P., India

Necrotizing fasciitis (NF) is potentially lethal life-threatening infection of skin and soft tissues.
Epidemic necrosis of the subcutaneous tissue and the fascia characterizes NF. Cellulitis is the
early presentation which appears first.In the early stages of NF the skin stays intact; this escort to
a deceptive benign mien. A wide variety of microbes causes NF. Trauma is typically followed by
this infection, albeit the urge scorn may be as trivial asan insect bite or a scrape. Hallmark sign
seen in NF is of severe local pain which is out of proportion to the size and type of wound. Often
caused by group of streptococcus virulent and toxin producing bacteria.Bacteria can be aerobic,
anaerobic, or mixed flora .Benign soft tissue infection often causes initial misinterpretation of
NF. The time to surgical debridement is the most important variable influencing mortality.
Exquisitely tender, red, and hot appearance of skin is perceived. Thus, clinical suspicions at high
degree is mandatory to avert consequences of potential disastrous. To evaluate patients with NF
disease orthopaedic surgeons are often distressed. The key to the treatment of this serious, often
life-threatening infection is auspicious diagnosis, broad-spectrum antibiotic therapy, and
intrusive surgical debridement of affected tissue. Increase in immunocompromised patients with
diabetes mellitus, cancer, alcoholism, vascular insufficiencies, organ transplants, HIV infection,
or neutropenia remits rise in frequency of NF.A exiguous manifest perceived NF syndrome are:Type I, or polymicrobial, Type II, or group A streptococcal, Type III gas gangrene, or clostridialmyonecrosis. The intent of this paper is to upsurge the knowledge and perceptive of NF.
Keywords:-Cellulitis, Benign soft tissue infection, surgical debridement, broad-spectrum
antibiotic therapy, gangrene.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus

National Conference on “Drug Discovery and Formulation Development” 23rd February 2016

PO357

EVALUATION OF PERIPHERAL ANALGESIC ACTIVITY OF BARK OF
ANNONA SQUAMOSA FROM GONDA REGION
Sandeep1, 2, Angshu Banargee4, Abhilasa3, Rahul Shukla5, Ashutosh Mishra2
1. Research Scholar, Department of Pharmaceutical Science, Jayoti Vidyapeeth Women’s
University, Jaipur.
2. A.N.D. College of Pharmacy, Babhnan, Gonda, Uttar Pradesh – 271 313
3. Department of Pharmaceutical Science, Jayoti Vidyapeeth Women’s University, Jaipur.
4. RAYAT Institute of Pharmacy, Chandigarh-Ropar-Jalandhar Highway, Railmajra, Distt.
Nawanshahr, Punjab - 144533
5. Amity Institute of Pharmacy, AUUP Lucknow campus, Uttar Pradesh.

The extracts of bark of Annona squamosa L. (Family Annonaceae) were investigated for
analgesic activity in formalin test and writhing test method. The dried power of bark extracted
with different solvents according to their polarity such as petroleum ether, chloroform, ethanol
and water. All the extracts given at a dose of 100 and 200 mg/kg b.w through orally. Extracts
shows dose dependent analgesic activity. Aqueous extract of Annona squamosal bark at a dose
of 200 mg/kg produce highest activity is both the models.
Key words: Annona squamosa, Analgesic, Formalin Test, Hot Plate, Indomethacin,
Pentazotocin.

Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus