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Advances in Biological Research 3 (1-2): 43-47, 2009

ISSN 1992-0067
© IDOSI Publications, 2009

Additive Main Effects and Multiplicative Interactions Analysis of


Yield Performances in Rice Genotypes under Coastal Saline Environments

A. Anandan, R. Eswaran, T. Sabesan and M. Prakash

Department of Agricultural Botany, Faculty of Agriculture,


Annamalai University, Chidambaram-608 002, Tamil Nadu, India

Abstract: Plant Breeders evaluate their germplasm in multi-environment to study the performance and
adaptation for specific or general environment. The objective of the present investigation was to analyse the
pattern of Genotype x Environment (G x E) interaction for grain yield of 46 genotypes by Additive Main effects
and Multiplicative Interaction (AMMI) model using the data generated from three saline stress environments
of east coastal region of Tamil Nadu, India. The results showed highly significant genotypic and G x E
interaction. The G x E interaction influenced the relative ranking of the genotypes across saline stress
environment condition. Among the genotypes tested, G45, G26, G27, G35 and G34 showed low interaction effect
with score nearest to zero with above average yield. While two genotypes, G36 and G19 exhibited high positive
interaction effect, gave the highest mean grain yield and is better suited to favourable environment. Similarly,
AMMI characterized the environments and identified E2 as a favourable environment for the better expression
of the trait studied.

Key words: Rice % Genotype x environment % AMMI % Yield

INTRODUCTION different environments to study genotype x environment


(G x E) interaction. To estimate the level of interaction of
Rice is considered moderately sensitive to salinity. genotypes to environments and to eliminate as much as
Higher population growth rate and the conversion of possible the unexplainable and extraneous variability
some highly productive rice cultivation lands for contained in the data, several statistically techniques
industrial and residential purposes, has pushed rice have been developed to describe G x E and measure the
cultivation to less productive area such as saline, stability of genotypes. For further detailed study on
drought and flood prone areas. However, cultivars current statistics used to test and measure genotype
adaptable to these abiotic stress areas were originated or stability are given by Lin et al. [3] , Piepho [4 ] and Laxmi
selected for these situations were agronomically poor and Gupta [5].
(traditional cultivars). Salt tolerance cultivars such as Since, G x E interaction is naturally multivariate, the
pokkali, cheriviruppu, nona bokra and SR26B are tall, Additive Main effects and Multiplicative Interaction
photosensitive, have poor grain quality and are low (AMMI) offers on appropriate first statistical analysis of
yielding [1]. After green revolution, several breeding yield trials that may have a G x E interaction [6]. The
programs were adapted to develop saline tolerant objectives of this study were to assess the extent of G x E
genotypes at IRRI [2] and other rice growing countries. interaction and to select the stable genotypes of rice in
The venture to develop high yielding genotype with very saline stressed environment over season.
good tolerance on par to traditional cultivars is still in
progress. MATERIALS AND METHODS
The developed cultivars should adapt to a wide range
of target environments, is the eventual goal of plant Forty six rice genotypes (Table 1) from different parts
breeders. Hence, pattern of response of genotypes is India were evaluated at Plant Breeding Research Farm,
studied by the plant breeders by testing genotypes in Faculty of Agriculture, Annamalai University, Annamalai

Corresponding Author: A. Anandan, Department of Agricultural Botany, Faculty of Agriculture,


Annamalai University, Chidambaram-608 002, Tamil Nadu, India
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Advan. Biol. Res., 3 (1-2): 43-47, 2009

Table 1: Grain yield (kg/plot) of 46 rice genotypes grown in three environments and the first IPCA scores for the G x E interaction effect as derived from
AMMI analysis
Genotype Genotype code E1 E2 E3 Genotype mean IPCA 1
NDR 9730015 G1 1.78 2.50 1.83 2.04 -0.051
NDRK 50001 G2 2.33 3.20 2.77 2.77 0.202
NDRK 50002 G3 2.10 2.70 1.88 2.23 -0.240
NDRK 50004 G4 4.23 4.79 4.31 4.44 -0.070
NDRK 50005 G5 2.76 3.71 3.07 3.18 0.147
NDRK 50007 G6 1.34 2.14 1.63 1.70 0.102
CSAR 520 G7 5.19 6.12 5.55 5.62 0.175
CSAR 518 G8 3.48 4.37 3.62 3.82 0.033
TR 2003-25 G9 4.27 4.77 4.40 4.48 -0.055
TR 2003-82 G10 1.60 2.07 1.58 1.75 -0.152
TR 2003-3 G11 5.20 5.68 5.20 5.36 -0.136
Bio 5-1 G12 4.46 5.06 4.49 4.67 -0.090
BM 5 G13 2.01 2.71 2.36 2.36 0.122
GGV-SAT-05-1 G14 2.21 2.85 2.44 2.50 0.035
RP 4679-3-2-38-1-1 G15 4.49 4.99 4.28 4.59 -0.251
CR 2069-16-1 G16 3.95 4.50 4.37 4.27 0.129
CR 2094-155-4 G17 4.22 4.56 3.90 4.23 -0.351
CR 2071-245-3 G18 5.40 6.38 5.86 5.88 0.241
CR 2092-88-1 G19 5.81 6.64 6.09 6.18 0.108
RP 4658-IR 52707-20-7-1-3-5-P1-4 G20 1.76 2.42 2.06 2.08 0.084
RP 4659-IR 59418-20-2-1-5-7-P5-1 G21 1.39 1.93 1.32 1.55 -0.157
MTU 2716 G22 5.54 6.33 5.79 5.89 0.077
MTU 1061 G23 3.58 4.34 3.87 3.93 0.096
CN 1266-19-16 G24 3.50 4.22 3.63 3.78 -0.003
CN 1266-26-177 G25 3.94 4.64 3.92 4.17 -0.096
CN 1266-9-6 G26 6.00 6.77 6.15 6.31 0.016
CN 1266-3-4 G27 4.88 5.43 5.04 5.12 -0.022
CN 1912 G28 3.70 4.41 4.18 4.09 0.194
CN 1914 G29 3.80 4.25 3.61 3.89 -0.247
CN 1913 G30 3.73 4.02 3.45 3.73 -0.336
CSR 13 G31 7.24 7.71 7.20 7.38 -0.164
CSR 27 G32 2.92 3.58 3.09 3.20 0.003
CST 7-1 G33 5.64 6.46 5.87 5.99 0.071
Jaya G34 5.56 6.24 5.74 5.85 0.011
Pant Kalanamak 3131 G35 5.49 6.17 5.65 5.77 0.001
Pant Dhan 102 G36 6.03 6.95 6.41 6.46 0.176
RP 4677-6-16-1-1-2-B G37 4.44 5.04 4.58 4.69 -0.030
RP 46768-5-1-7-1-2-3 G38 4.49 5.22 4.67 4.80 0.024
MAUB 13 G39 1.60 2.39 1.68 1.89 -0.021
RP 4347-TSH-48-6-2-2-3-B G40 5.59 6.33 5.67 5.87 -0.030
RP 4339-MSC-55-1-4-2-3-8-B G41 4.18 5.13 4.44 4.58 0.115
RP 4676-23-6-1-2-5-B G42 4.66 5.52 5.03 5.07 0.162
RP 4343-4-2-2-1-3-1-B G43 4.40 5.13 4.67 4.73 0.078
RP 4677-16-6-1-1-2-1-2 G44 3.40 3.97 3.32 3.57 -0.163
RP 4343-18-2-1-1-1-1-B G45 4.06 4.84 4.22 4.37 0.023
TRY 1 G46 4.07 5.05 4.53 4.55 0.241
Environment mean 3.97 4.66 4.12 4.25
IPCA 1 -0.777 0.201 0.576

Nagar, located at East coastal region of Tamil Nadu, India whole plot was used to determine the yield potential of
with soil pH of 8 to 8.5 and EC of 2.51 to 2.8 dSmG1. The each genotype.
crop was evaluated in three seasons viz., E1 (Kharif, 2006),
E2 (Kharif, 2007) and E3 (Rabi, 2007). For all the trials, Statistical Analysis: AMMI is a unified approach that
the design used was RCBD with three replications. fits the additive main effects of genotypes and
The plot had 10 sq.m with spacing of 20 cm between environments by the usual analysis of variance and
rows and 20 cm between plants. Management practices then describes the non-additive parts by principal
were uniformly adapted to all seasons as per the component analysis fitted to the AMMI model according
recommendation for rice in the irrigated condition. The to the following equation.

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Advan. Biol. Res., 3 (1-2): 43-47, 2009

Yge = µ + "g + $e + E 8n ãgn *en + 2ge + 0ger Table 2: AMMI analysis of variance for grain yield of 46 rice genotypes
Source df SS MS F

Where, Yge is the yield of genotype g in environment e, Treatments 137 905.60 6.61 216.78**
Genotypes 45 865.30 19.23 630.56**
µ is the grand mean yield, "g is the genotype mean
Environments 2 36.50 18.24 5.43**
deviation, $e is the environment mean deviation, 8n is the
Interactions 90 3.90 0.04 1.42*
eigen value of the nth principal component, ãgn and *en are IPCA 1 46 2.90 0.06 2.04**
the genotype and environment scores for the nth principal Residuals 44 1.00 0.02 0.77
component; 2ge is the residual and 0ger is the random error Error 270 8.20 0.03
[6 , 7]. Total 413 934.00 2.26
The additive part of the AMMI model (µ, "g, $e) is *P<0.05 and **P<0.01
estimated from an analysis of variance and the
multiplicative part (8n, ã gn and * )en from a principal 25.64% with 51% and 49% of the degrees of freedom
component analysis. The interaction between any respectively for G x E interaction. The first principal
genotype and environment can be estimated by component factor has a high contribution to the
multiplying the score for the interaction principal interaction sum of squares while the residual is small.
component axis (IPCA) of a genotype (8n 0.5 x ãgn) by an This indicates that one fundamental factor affects G x E
environment IPCA score (8n0.5 x *en) [8]. Furthermore, interaction; this could be either genotypic or
Gauch and Zobel [9] proposed an approach to estimate environmental in nature [11].
the relevant portion of G x E for the trait studied to avoid The errors from uncontrolled variation were
spurious interpretation of statistical results. Partitioning calculated using the methods described by Gauch and
the errors from uncontrolled variation (noise) out of the Zobel [9]. The interaction of the grain yield contained
total G x E sum of squares (SS) is important because most 69% noise and 30.77% real structure, with the relevant
of the noise appears in the interaction. Noise SS, real (target) variation being 95.68% of the treatment sum of
structure SS and target relevant variation percentage were squares. Treatment sum of squares variation explained
calculated as described by Gauch and Zobel [9]. The above target percentages that would be attributed to
target percentage of the relevant variation explained by noise. The first IPCA component explained 95.87% of
IPCA in the AMMI analysis should equal SS (relevant the treatment sum of squares (2.9 + 865.3) / 905.6) which
variation) / SS (treatment). was very close to the target percentage sum of squares
explained (95.68%).
RESULTS AND DISCUSSION Data presented in Table 1 show that the mean yield
for 46 genotypes evaluated in three environments, plus
Bartlett’s test indicated homogenous error variance the genotype and environment means and first IPCA
for the trait yield in each of three environments and scores. The grain yield ranged from 1.32 kg/plot to
allowed to proceed further for pooled analysis across 7.71 kg/plot with an average of 4.25 kg/plot. The AMMI
environment. ANOVA across environments detected 1 estimated yield for any genotype in any location/season
significant variation among genotypes and for the G x E may be calculated from the biplot, as described by
for the trait grain yield (Data not shown). The analysis of Zobel et al. [6]. The biplot (Fig. 1) captured 99% of the
variance of AMMI (Table 2) showed that the mean sum of treatment sum of squares. Genotypes with IPCA 1 scores
squares due to treatments, genotypes, environments and near zero had little interaction across environments, while
genotype x environment interaction were significant, the genotypes with large IPCA 1 scores, either positive or
indicating broad range of diversity existed among negative direction were highly interactive. Similarly,
genotypes [10]. Significance of the environments locations/seasons with IPCA 1 scores near zero had
indicated distinctness of intrinsic factors in different little interaction across genotypes and low discrimination
environment. The significance exhibited by G x E among genotypes. Genotype and location combinations
interaction indicated that each of the genotype interacted with IPCA 1 scores of the same sign produced positive
differentially in various environment tested. The specific interaction effects, whereas combinations of
multiplicative variance of the treatment sum of squares opposite sign had negative specific interactions [12-14].
due to G x E interaction was partitioned into IPCA 1 and In Fig. 1 for any genotype-environment combination,
residual variation. About 74.3% of the interaction sum of the main effect equals the genotype mean plus the
squares was explained by IPCA 1 and residual explained environment mean minus the grand mean and the

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Advan. Biol. Res., 3 (1-2): 43-47, 2009
0.6 E3

0.4

G4 6 G18
0.2 G2 G28 E2 G7 G36
G6 G5 G4 2
G13 G16 G41
G2 0 G2 3 G43 G2 2 G33G19
G14 G8 G45 G38 G3 5 G3 4 G2 6
0.0 G3 9 G3 2 G2 4 G2 7
G9 G4 G37
IPCA 1
G1 G4 0
G2 1 G25 G12 G11
G10 G4 4 G3 1
-0.2
G3 G3 5 G15
G3 0 G17
-0.4

-0.6

-0.8 E1
1.5 2.0 2.5 3.0 3.5 4 .0 4.5 5.0 5 .5 6.0 6.5 7.0
MEA N

Fig. 1: AMMI – 1 biplot for grain yield of rice, showing both main and interaction effects for both genotypes and
environments

interaction is the genotype IPCA score times the mean and high IPCA score. Similar signs of IPCA 1 score
environment ICPA score [15]. For example, G5 grown in for both genotype and environment imply positive
environment E2 had a main effect of 3.18 + 4.66 - 4.25 = interaction and thus it attributes to higher yield of
3.59 kg/plot and an interaction effect of (0.147) x (0.201) = genotype at that particular environment [13 , 17]. For
0.029 kg/plot. Therefore, the AMMI model gives a grain instance, the E2 is the most favourable for the genotypes,
yield estimation of 3.59 kg/plot. This fits well the observed G46, G41, G43, G42, G7, G22, G18, G33, G19 and G36. The
value of 3.71 kg/plot. favourable response environment E2 might have been
From the Fig. 1, it is clear that, genotypes G17, G25 due to evenly distribution of rainfall, this probably
and G16 differ only in interaction effect and not in main reduced the salt toxicity by leaching.
effect, while G39, G32, G24, G45, G27, G35, G34 and G26 The AMMI analysis appeared as a more useful
showed little G x E interaction on IPCA 1 and grouped methodology in quantifying G x E and have greater insight
together along the abscissa, although they differ into the magnitude and nature of G x E interaction. This
dramatically in main effect. The difference exhibited by model is effective in characterizing genotypes and
genotypes over interaction and main effect might have environments as well as interactive and discriminative
been due to the factor salinity stress. The genotypes, pattern of genotypes and environments respectively.
which had IPCA score nearest to zero, are G24, G26, G27,
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