CELL LINE

DEVELOPMENT
(CHINESE HAMSTER OVARY CELL)
NAME
AMIRUL HAFIZI BIN HASHIM
NIK ABD HAFIIDZ BIN NIK ABDUL MALEK
AMIRAH BINTI ADNIRUDDIN
NOR HAFIZA IZZATI BINTI WAHAB
GROUP: EH242/6E
LECTURER: PUAN SYAZANA BINTI PAUZI
DATE SUBMITTED: 17 MAC 2016

STUDENT ID
2014265264
2014688106
2014819826
2014627112

1.0

Introduction

The approval of Chinese hamster ovary (CHO)-derived tissue plasminogen activato

in 1986 revolutionized medicine and raised the possibility of using mammalian cell
culture for the manufacturing of protein therapeutic products. The reason why CHO
cells remained as the preferred mammalian cell line for the production of
recombinant protein therapeutic are (lai et.al., 2013):

HO cells are capable of adapting and growing in suspension culture which is

ideal for large scale culture in the industry

CHO cells pose less risk as few human viruses are able to propagate in them
CHO cells can grow in serum-free and chemically defined media which

ensures reproducibility between different batches of cell culture

CHO cells allow post translational modifications to recombinant proteins
which are compatible and bioactive in humans

Chinese Hamster Ovary (CHO) originated in the deserts of Northen China. (CHO)
cells

are

the

most

biopharmaceuticals.

common

cells

applied

in

commercial

production

of

It is preselected as host cell lines since its gives maximum

attainable yields on product.

2.0

ORIGINATOR

Pharmaceutical Cell Line Development was develop by ProBioGen to fulfill clients

needs. ProBioGen is a technology provider with focus on cell line engineering,
process development and Good Manufacturing Practice (GMP). CHO-DG44 and CHOK1 was use by ProBioGen as starter cell lines and both have been selected for most
efficient growth in chemically defined media and for optimized biopharmaceutical
manufacturing. It also immense adaptive ability and safer than actual human cell
lines because most pathogenic virus includes HIV do not infect these cells.
3.0

PROCESS SYNTHESIS

Figure 1: A schematic of steps involve in cell line development for production of

recombinant CHO cell (Karthik et. al)
4.0

USAGE/SERVICES

- Use as gene, transcript and signal peptide optimization also cloning.

- Profilling of product lines.
- Analysis of expression stability.
- Fast cell line development.
- Product extension include monoclonal antibodies, antybody fragments, enzymes
and growth factors.
5.0

STATE OF PRODUCT

- In liquid form
6.0

DOWNSTREAM PROCESS FOR CELL LINE DEVELOPMENT

In downstream processing, it focusses on yield, productivity and purity. A

certain optimization in downstream process has been done to ensure high-end

product quality. In cell line development, downstream process involved filtration
process, purification process and analytical assay development

DSP of Cell line development

Fermentation

Filtration

Analytical
Assay

Purification
(chromatography

Storage
(Cyropreservati

Extended
Product

Chinese Hamster Ovary (CHO) cells are the most common cells applied in
commercial production of biopharmaceuticals. The original CHO cell line isolated in
the 1950s gave rise to a range of genetically different progeny, such as K1-, DukX
B11-, DG44- cell lines and others. The choice of CHO cell line can affect target
protein quality and achievable yield.
7.0 REFERENCE
Tingfeng Lai, Yuansheng Yang and Say Kong Ng (2013). Advances in Mammalian Cell
Line

Development

Technologies

Pharmaceuticals (Basel). 6(5): 579603.

for

Recombinant

Protein

Production.

Karthik P. Jayapal, Katie F. Wlaschin, Wei-Shou Hu &

Miranda G. S. Yap (2007).

Recombinant Protein Therapeutics from CHO Cells - 20 Years and Counting.