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LUNA

The Worlds #1 HPLC Column

LUNA

#1 FOR METHOD DEVELOPMENT WORLDWIDE

TABLE OF CONTENTS
Overview ....................................................................................... 2
Reproducibility Validated .............................................................. 4
pH Stability 1.5-10 ........................................................................ 6
Luna C18, C8, C5 ......................................................................... 8
Luna Phenyl-Hexyl ...................................................................... 18
Luna Cyano [CN] ........................................................................ 20
Luna Amino [NH2] ....................................................................... 22
Luna LC/MS and ........................................................................ 24
Combinatorial Chemistry
Luna Capillary LC ....................................................................... 25
Luna Preparative LC ................................................................... 26
Bonded Phases .......................................................................... 27
Ordering Information .................................................................. 28

Luna C18(2) 3 column gave us the column

I was not able to achieve


Overview
Wouldn't you like to boast about the performance of your HPLC
column? Many Luna customers do. Our passion for manufacturing
the best HPLC columns, has resulted in Luna - one of the World's
most popular columns. Luna represents some of the highest quality
and performance standards in HPLC. Take a moment to explore
Luna, we are certain you will discover amazing HPLC column
performance like so many of our customers.

Incredible Silica Smoothness


Luna silica is very smooth and spherical, as illustrated in the Luna
versus Zorbax silica comparisons below. The likelihood of silica
particle shearing and breakage during bonding and packing is very
low; thus, Luna columns have high efficiencies and long column
lifetimes.

Superior Silica For Long Column Lifetimes


Silica quality is not simply the result of Luna's 99.999% purity.
Meticulous care is given to quality control over silica smoothness,
pore structure and pore consistency leading to a highly advanced
HPLC silica with:
Low percentage of "fines" from damaged silica
particles stabilizing the column bed and leading
to lower backpressures and enhanced column
performance and lifetimes
High column bed stability enhanced by
particle shape uniformity

Phases to separate any compound


LUNA : Si, C5, C8, C8(2), C18, C18(2), Phenyl-Hexyl, CN, NH2
Zorbax 5 SB-C18 Silica

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Luna 5 C18 (2) Silica

stability and chromatographic resolution

with any other product.

(Scott T. Stone, Polaroid Corporation, USA)

pH Stability: 1.5 to 10.0*


Luna was one of the first columns to provide greater flexibility and
control in method development at pH extremes. More importantly,
Luna provides good peak shape independent of mobile phase pH.

Reproducibility Validated
A Materials Validation Document showing the test results for the
batch of material and the individual column accompanies each
Luna column. This data ensures column-to-column and batch-tobatch reproducibility.
Versatile Configurations
Capillary-LC to preparative/process chromatography columns and
bulk material are available to meet any separation need.

*Luna CN is stable from pH 1.5 to 7.0


Luna NH2 is stable from pH 1.5 to 11.0

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This Luna silica column keeps

going and going

Reproducibility Validated
It is tough to meet Luna quality standards. The Tanaka plots below
illustrate the reproducibility of 13 different quality control parameters
for 9 batches of Luna material - nearly no variation is observed.
Each batch represents hours of quality control testing for over 25
different parameters. We know chromatographers rely on Luna for
rugged, reproducible methods worldwide; therefore, an extensive
Materials Validation Document (MVD) accompanying each column
confirms consistent column specifications and consistent
performance.

Particle Size

Surface Coverage

Particle Size Distribution

Reliable Performance
Almost no variation is observed among the batches of Luna. Figure
1 shows quality control test data designed to monitor the slightest
differences that may affect reproducibility - particle shape and
smoothness, porosimetry, bonding consistency and pH stability.
Figure 2 shows bonding reproducibility test data from 9 Luna
batches. Since differences in bonded phase coverage can show
selectivity changes, each Luna batch undergoes 6 tests for bonding
reproducibility.

Pore Size (x100)

Carbon Load %

3Figure 1 - Silica
Reproducibility. Batch No:
Metal (ppm/10)

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Surface Area (x100m2/g)

and going.

IT HAS HAD OVER 400 RUNS OF EXTRACTED PLASMA AND THE PRESSURE HAS NOT RISEN ONE BIT.

(David Fry, NYU Medical Center, USA)

Column to Column Reproducibility


The chromatograms in Figure 3 show consistency of inertness (blue)
and hydrophobicity (red) for Luna 5u C18(2) columns from 9 different
batches. Almost no variation is observed.

Inert test

Selectivity
of Basic Drugs, K3/K2

Hydrophobicity

Batch No.
1
2
3
4
5
6

Selectivity
of Basic Drugs, K2/K1

Hydrogen Bonding

7
8
9

3Figure 2 - Bonded Phase


Reproducibility. Batch No:

Inert Test
Hydrophobicity

5Figure 3 - Column-to-column
reproducibility for 9 batches of Luna

SRM-869

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LUNA C18(2) columns gave good column efficiency, excellent column symmetry,

and most importantly, superior

pH Stability: 1.5 - 10.0


Luna was one of the first columns to provide an extended pH range
of 1.5 to 10* for most of its phases (Figure 4). The broad pH stability
range is partly achieved by the density of the bonded phase. Luna
C18(2) columns are exhaustively bonded to show great peak shape
independent of pH, indicating free silanols are well shielded from
the analyte compounds (Figure 5). Try Luna! It will consistently
provide good peak asymmetry across a wide pH range.
* Luna CN is stable from pH 1.5 to 7.0; Luna NH2 is stable from pH 1.5 to 11.0

tR,K, Symmetry of Toluene and Pyridine

Hours of Exposure
Test Conditions: Column stability tested under highly basic conditions. Continuous
flush in 20mM Na2HPO4 (pH 10.0) in Water/Acetonitrile, 50:50
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Hours of Exposure
Test Conditions: Column stability tested under highly acidic conditions. Continuous
flush in 0.1% TFA (pH 1.5) in Water/Acetonitrile, 50:50

Efficiency of Toluene p/col

pH10.0 pH1.5

tR,K, Symmetry of Toluene and Pyridine

Efficiency of Toluene p/col

Figure 4 - Efficiency and peak asymmetry stability at pH 10.0 and 1.5 for
LUNA 5 C18(2)

column stability when using high pH mobile phases.

(Wie Zhou, Ph.D., MRACI, Faulding Pharmaceuticals, Australia)

Sharp Peaks For Basic Compounds at High pH


With Luna columns it is possible to work above the pKb of many
basic compounds, with the compound in a "neutral charge state",
thus the basic compound becomes more hydrophobic (less polar).
This can result in improved retention, peak shape, and
reproducibility. Figure 6 illustrates how a pH adjustment can be
used to alter selectivity and retention. A wide operational pH range
extending to 10.0 is useful for basic compounds.

Figure 5 - Average peak shape over pH range: Waters


Symmetry 5 C18 = 2.26, Phenomenex LUNA 5 C18(2) = 1.43

Figure 6-Optimizing resolution and selectivity with mobile phase pH increases method
development flexibility.
CONDITIONS FOR SEPARATIONS AT RIGHT4
Column: Luna 5 C18
Dimensions: 150 x 4.6mm
Order No.: 00F-4041-E0
Mobile Phase: Buffer/ Acetonitrile, (95:5)
pH as shown
Flow Rate: 1.0mL/min
Temperature: 40C
Detection: UV @ 270nm
Sample: 1. Sulfanilic Acid
2. Sulfamerazine
3. Sulfisoxazole
4. Sulfapyridine

pH 1.25

Poor sample stability at low pH*

pH 3.0

pH 5.0

Separation, but run time too long

pH 7.0

Good separation, change


in elution order

Waters Symmetry 5 C18


Phenomenex Luna 5 C18(2)
3

Optimal separation and run time*

N = 5 for each data point

Asymmetry of Amitriptyline

pH 9.0

0
2

pH 10.0

Buffer pH
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We are finding your columns


very useful for the separation
Luna C18(2), C8(2), C5
Luna has found a place as one of the World's top reversed phase
columns because it ensures two important chromatographic
properties: resolution and peak shape. The high efficiencies and
bonded phase surface coverage provide for sharp peaks. Whether
you need a column for USP methods or just general method
development, Luna C18(2) and C8(2) should be your first choice
every time. The result:
Free exposed silanols virtually eliminated
by complete bonding and endcapping
Sharp peak shape for good method sensitivity
pH stable from 1.5 to 10.0 for over 10000 hours

CONDITIONS SAME FOR BOTH COLUMNS:


Dimensions: 150 x 4.6mm
Mobile Phase: A)Water with 0.05% TFA
B)Acetonitrile with 0.05% TFA
Gradient: A/B (95:5) to A/B(5:95) in 10 min
Flow Rate: 1.0mL/min
Detection: UV @ 254 nm
Injection: 10L
Sample: Doxylamine (0.12mg/mL)

Phenomenex
LUNA 5 C18(2)
Peak width = 1.82 sec

Waters
XTerra 5 RP18
Peak width = 3.71 sec

The proof is in the peaks - see for yourself.

App ID 1100

PEAK ASYMMETRY: A COMPARISON OF C18 COLUMNS

CONDITIONS SAME FOR BOTH COLUMNS:

Dimensions:
Mobile Phase:
Flow Rate:
Detection:
Sample:

Phenomenex
LUNA 5 C18(2)

Waters
Symmetry 5 C18

Pyridine Asymmetry
As =1.44

Pyridine Asymmetry
As =2.27

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150 x 4.6mm
Water/Acetonitrile (50:50)
1.0mL/min
UV @ 254 nm
1. Pyridine
2. Phenol
3. Toluene

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POLAR, ACIDIC DRUGS


Phenomenex LUNA 3 C18(2)
Waters Symmetry 3.5 C18

BASIC COMPOUNDS
Phenomenex LUNA 5 C18(2)
Macherey Nagel Nucleosil 5 C18

App ID 12903

App ID 12904

CONDITIONS SAME FOR BOTH COLUMNS:

Dimensions: 75 x 4.6mm
Mobile Phase: 20mM KH2PO4,
pH 6.0/Acetonitrile
(70:30)
Flow rate: 0.75mL/min
Detection: UV @ 202nm
Sample: 1. Tolmetin
2. Naproxen
3. Diflunisal
4. Fenoprofen
5. Indomethacin
6. Ibuprofen

App ID 14316 App ID 14317


CONDITIONS SAME FOR BOTH COLUMNS:

Dimensions: 150 x 4.6 mm


Mobile Phase: 20mM Potassium
phosphate, pH 2.5
/ Acetonitrile (75:25)
Flow Rate: 1.5 mL/min
Temperature: 30C
Detection: UV @ 210nm
Sample: 1. Maleic acid
2. Triprolidine*
3. Chlorpheniramine*
4. Diphenhydramine*
*Peaks 2-4 adsorb on
Nucleosil C18

App ID 12907 App ID 12910

NON-POLAR, BASIC DRUGS


Phenomenex LUNA 5 C8
Agilent Technologies Zorbax 5 XDB-C8

HYDROPHOBIC, ACIDIC COMPOUNDS


Phenomenex LUNA 5 C18(2)
Thermo Hypersil HyPURITY Elite 5 C18

CONDITIONS SAME FOR BOTH COLUMNS:

Dimensions: 150 x 4.6mm


Mobile Phase: 10mM KH2PO4,
pH 7.0/Acetonitrile/
Methanol, (16:74:10)
Flow Rate: 1.0mL/min
Detection: UV @ 210nm
Sample: 1. Desipramine
2. Nortriptyline
3. Doxepin
4. Imipramine
5. Amitriptyline

App ID 14314 App ID 14315


CONDITIONS SAME FOR BOTH COLUMNS:

Dimensions: 150 x 4.6mm


Mobile Phase: 20mM Potasssium
phosphate, pH 2.5
/ Acetonitrile (75:25)
Flow Rate: 1.5mL/min
Temperature: 30C
Detection: UV @ 254nm
Sample: 1. p-Hydroxybenzoic acid
2. Sorbic acid*
3. Benzoic Acid*
4. Salicylic acid
5. p-Toluic acid
* Sorbic acid and Benzoic
acid co-elute on HyPURITY Elite

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App ID 4099

DIET SODA POP


Column:
Dimensions:
Order No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

CAFFEINE AND METABOLITES


Column:
Dimensions:
Order No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

1
2

App ID 12921
Luna 5 C18
150 x 4.6mm
00F-4041-E0
50mM Sodium acetate/
Acetonitrile/Methanol (92:4:4)
1mL/min
22C
UV @ 273nm
1.
Theobromine
2. Paraxanthine
3. Theophylline
4. Excipient
5. Caffeine

5
4

10

15

a- AND b-ACIDS IN HOP EXTRACT

Luna 3 C18(2)
30 x 4.6mm
00A-4251-E0
Sodium Acetate Buffer w/TEA
and approx. 15% acetonitrile, pH 4.6
w/10% v/v sulfuric acid
2mL/min
22C
UV @ 214nm
1. Caffeine
2. Potassium benzoate
3. Aspartame

20 min

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Column:
Dimensions:
Order No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

App ID 14313
Luna 5 C18(2)
250 x 4.6mm
00G-4252-E0
Methanol with 0.1% H3P04
/Water with 0.1% H3P04 (90:10)
1.5mL/min
30C
UV @ 314nm
1. Cohumulone
2. Ad-+ humulone
3. Colupulone
4. Ad-+lupulone

App ID 2579

METHYLATED XANTHINES
Column:
Dimensions:
Order No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

Luna 5 C8
150 x 4.6mm
00F-4040-E0
Methanol/20mM KH2P04,
pH 9.0 (99.9:0.1)
1mL/min
22C
UV @ 254nm
1. 8-Chlorotheophylline
2. Theophylline
3. 1,7-Dimethylxanthine
4. Caffeine

of close eluting peaks.


(Mythili Nagarajan, Enzymatic Therapy, USA)

ESTRADIOL-USP METHOD
Column:
Dimensions:
Order No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

PHENYLEPHRINE HYDROCHLORIDE INJECTION-USP METHOD

App ID 1313

App ID 10893
Luna 5 C18(2)
150 x 4.6mm
00F-4252-E0
Acetontrile/Water (55:45)
1mL/min
30C
UV @ 254nm
1. Ethylparaben
2. Estrone
3. Estradiol

COCAINE-NARCOTIC DRUG

App ID 3246

HYDROCORTISONE CREAM-USP METHOD

Column: Luna 5 C18(2)


Dimensions: 250 x 4.6mm
Order No.: 00G-4252-E0
Mobile Phase:Water/Methanol with 1.1%
1-Octanesulfonic
acid pH 3.0 (50:50)
Flow Rate: 1mL/min
Temperature: 22C
Detection: UV @ 280nm
Sample: 1. Phenylephrine hydrochloride
2. Epinephrine bitartrate

Column:
Dimensions:
Order No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

Luna 5 C18(2)
150 x 4.6mm
00F-4252-E0
Phosphate Buffer, pH2.5/
Acetonitrile (75:25)
1mL/min
30C
UV @ 233nm
1.Cocaine Hydrochloride

Column:
Dimensions:
Order No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

App ID 3271
Luna 5 C18(2)
250 x 4.6mm
00G-4252-E0
Water/Acetonitrile (75:25)
2mL/min
30C
UV @ 254nm
1. Hydrocortisone

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Luna's long column lifetime and low-pressure characteristics

are essential to us
in ensuring reproducibility and stability
EPA 8330 - EXPLOSIVES
Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

11

7
2

10
12

14
13

GINGER PUNGENTS

10

12

14

App ID 14263
Luna 5 C18(2)
250 x 4.6 mm
00G-4252-E0
Methanol/Water (55:45)
1mL/min
35C
UV @ 254 nm
1. HMX
2. RDX
3. 1,3,5-Trinitrobenzene
4. 1,3-Dinitrobenzene
5. Tetryl
6. Nitrobenzene
7. 2,4,6-Trinitrotoluene
8. 4-Amino-2,6-Dinitriotoluen
9. 2-Amino-4,6-Dinitrotoluene
10. 2,6-Dinitrotoluene
11. 2,4-Dinitrotoluene
12. 2-Nitrotoluene
13. 4-Nitrotoluene
14. 3-Nitrotoluene

EPA 8310 - POLYNUCLEAR AROMATIC HYDROCARBONS (PAHs)


Column:
Dimensions:
Order No:
Mobile Phase:
Gradient:
Flow Rate:
Temperature:
Detection:
Sample:

App ID 14269
Luna 5 C18(2)
250 x 4.6 mm
00G-4252-E0
A:Water B:Acetonitrile
A/B (25:75) to 100% B in 25 min
2mL/min
22 C
UV @ 254 nm
1. Naphthalene
2. Acenaphthalene
3. Fluorene
4. Phenanthrene
5. Anthracene
6. Fluoranthracene
7. Pyrene
8. Benzo(a)anthracene
9. Chrysene
10. Benzo(e)pyrene
11. Benzo(b)fluoranthene
12. Benzo(k)fluoranthene
13. Benzo(a)pyrene
14. Dibenzo(a,h)anthracene
15. Benzo(g,h,i)perylene
16. Indenol(1,2,3-c,d)pyrene

16

App ID 12960
Luna 5 C18(2)
250 x 4.6 mm
00G-4252-E0
A: Water B: Acetonitrile
A/B (55:45) to A/B (50:50)
in 8min, A/B (35:65) in 15min,
A/B (10:90) in 40min
Flow Rate: 1mL/min
Temperature: 50C
Detection: UV @ 282nm
Sample: 1. 6-Gingerol
2. 8-Gingerol + isomer
3. 6-Shogaol
4. 10-Gingerol
5. 8-Shogaol
6. 10-Shogaol

Column:
Dimensions:
Order No:
Mobile Phase:
Gradient:

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COLD MEDICINE
Column:
Dimensions:
Order No:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
Sample:

App ID 2578
Luna 5 C8(2)
150 x 4.6 mm
00F-4249-E0
Methanol/Acetonitrile
with 0.1% H3PO4/ Water
with 0.1% H3PO4 and
0.1% Heptane
Sulfonate (40:10:50)
1.5mL/min
22C
UV @ 214nm
1. Acetaminophen
2. Pseudoephedrine
3. Benzoic acid
4. Chlorpheniramine
5. Dextromethorphan

WATER SOLUBLE VITAMINS


Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

PHARMACEUTICAL PRESERVATIVES
Column:
Dimensions:
Order No:
Mobile Phase:

Gradient:
Flow Rate:
Temperature:
Detection:
Sample:

App ID 3305
Luna 5 C18(2)
150 x 4.6 mm
00F-4252-E0
20mM Potassium Phosphate,
pH 3.0/Acetonitrile (95:5)
1.5mL/min
22C
UV @ 214 nm
1. Thiamine
2. Cyanocobalamin
(Vitamin B12)
3. Ascorbic acid
4. Pantothenic acid
5. Niacinamide
6. p-Aminobenzoic acid

POLYAMINES

App ID 1141
Luna 5 C5
150 x 4.6 mm
00F-4043-E0
A:0.5% Acetic acid in water/
Acetonitrile (80:20)
B: 0.5% Acetic acid in water/
acetonitrile (20:80)
A/B (100:0) to A/B (0:100) in 30min
1mL/min
25C
UV @ 254 nm
1. Propylparaben impurity
2. Benzyl alcohol
3. Phenol
4. Benzoic acid
5. Methylparaben
6. Benzaldehyde
7. Ethylparaben
8. Propylparaben

PYRONES

Column:
Dimensions:
Order No:
Mobile Phase:
Gradient:
Flow Rate:
Detection:
Temperature:
Injection:
Sample:

Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:

App ID 1288
Luna 5 C18 + SecurityGuard
250 x 4.6mm
00G-4252-E0 + KJ0-4282 + AJ0-4287
A: 93% Methanol in Water
B: 65% Acetonitrile in Water
A/B (0:100) to A/B (100:0) in 28
minutes to A/B (0:100) in 40 minutes
1.5mL/min
360nm Excitation, 530nm Emission
35C
20L
Dansyl derivatized
1. Putrescine
2. DHX
3. DHP
4. Spermidine
5. Spermine
6. Homospermine
7. Methylethylhomospermine
8. Diethylhomospermine (DEHOP)

App ID 1162
Luna 5 C8
150 x 4.6mm
00F-4040-E0
0.05% Phosphoric acid/Methanol (75:25)
1mL/min
UV @ 270nm
25C
1. 5-Hydroxy-2-hydroxymethyl
-4-pyrone (Kojic acid)
2. 3-Hydroxy-2-methyl-4-pyrone
3. 4-Hydroxy-6-methyl-2-pyrone
4. 2,6-Dimethyl-gamma-pyrone

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LORAZEPAM TABLETS-USP METHOD


Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

HORMONES
Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

App ID 3253
Luna 5 C18(2)
250 x 4.6 mm
00G-4252-E0
Water/Methanol/Acetic acid (54:44:2)
2mL/min
22C
UV @ 254 nm
1. Lorazepam

MINOXIDIL-USP METHOD

App ID 9903
Luna 5 C18
100 x 4.6 mm
00D-4041-E0
Water/Tetrahydrofuran/Acetonitile
(60:22.5:17.5)
1.2mL/min
40C
UV @ 244 nm
1. Norgestrel
2. syn-Norgestrel oxime
3. anti-Norgestrel oxime
4. Norgestrel acetate
5. syn-Norgestimate
6. syn-Norgestrel oxime diacetate
7. anti-Norgestimate
8. anti-Norgestrel oxime diacetate

IMPIRAMINE-USP METHOD

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Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:

Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:

App ID 3266
Luna 5 C18(2)
250 x 4.6mm
00G-4252-E0
Methanol/Water/Acetic acid with 7mM
Docusate sodium pH 3.0 (69.3:29.7:1)
1mL/min
UV @ 254nm
22C
1. Medroxyprogesterone acetate
2. Minoxidil

App ID 3255
Luna 5 C18(2)
250 x 4.6mm
00G-4252-E0
0.06M Sodium perchlorate, pH 2.0/
Acetonitrile/Triethylamine (62.5:37.5:0.1)
1.5mL/min
UV @ 269nm
22C
1. Imipramine
2. Desipramine

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NAPROXEN TABLETS-USP METHOD


Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

App ID 3282
Luna 5 C18(2)
150 x 4.6 mm
00F-4252-E0
Acetonitrile/Water/Glacial acid, pH
3.0 (50:49:1)
1.2mL/min
22C
UV @ 254 nm
1. Naproxen
2. Butyrophenone

FATTY ACIDS
Column:
Dimensions:
Order No:
Mobile Phase:

Gradient:
Flow Rate:
Detection:
Temperature:
Sample:

8
6
3
4

2
1

GUAIFENESIN TABLETS-MODIFIED USP METHOD


Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

App ID 3267
Luna 5 C18
100 x 4.6 mm
00D-4041-E0
Water/Methanol/Acetic acid
(60:40:1.5)
2mL/min
22C
UV @ 276 nm
1. Guaifenesin
2. Benzoic acid

10

12

14

App ID 1258
Luna 5 C8(2)
150 x 4.6mm
00F-4249-E0
A: Acetonitrile
B: Water (18Mohms DI)
A/B (70:30)) to A/B (90:10) in 10min, A/B
(90:10) to A/B (70:30) in 2min, hold for 4min
0.3mL/min
Evaporative Light Scattering (ELSD)
22C
1. Lauric acid
2. Myristic acid
3. Palmitoleic acid
4. Linoleic acid
5. Palmitic acid
6. Oleic acid
7. Heptadecanoic acid
8. Stearic acid

min

ALBUTEROL TABLETS-USP MEHOD


Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:

App ID 3260
Luna 5 C18(2)
150 x 4.6mm
00F-4252-E0
Methanol/Water with 5mM Hexane sulfonic
acid and 1% Glacial acetic acid (40:60)
1.5mL/min
UV @ 276nm
22C
1. Albuterol

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CEPHALOSPORIN ANTIBIOTICS
Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

PENICILLIN ANTIBIOTICS
Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

App ID 1222
Luna 5 C18
150 x 4.6 mm
00F-4041-E0
Water with 0.5% Acetic acid/
Acetonitrile (85:15)
1mL/min
22C
UV @ 254 nm
1. Cefadroxil
2. Cephalexin
3. Cephradine
4. Cefachlor

SUN SCREENS

App ID 1215
Luna 5 C8
150 x 4.6 mm
00F-4040-E0
Methanol/25mM KH2P04, pH 4.6
(55:45)
1mL/min
22C
UV @ 254 nm
1. Amoxicillin
2. Ampicillin
3. Penicillin G
4. Penicillin V

ANTIOXIDANTS

16 x Phenomenex x Tel. 310.212.0555 x Fax 310.328.7768 x Web www.phenomenex.com

Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

Column:
Dimensions:
Order No:
Mobile Phase:
Gradient:
Flow Rate:
Temperature:
Detection:
Sample:

App ID 1239
Luna 5 C18
250 x 4.6mm
00G-4041-E0
Methanol/Water (80:20)
1.5mL/min
22C
UV @ 240 nm
1. Benzophenone 3
2. DCHP
3. Parsol MCX
4. Homosalate 2
5. Octyl salicylate
6. Homosalate 1
7. Homosalate

App ID 1283
Luna 5 C18(2)
100 x 4.6mm
00D-4252-E0
A: Acetonitrile
B: Phosphate Buffer
A/B (30:70) to A/B (70:30) in 5min
1mL/min
22C
UV @ 254 nm
1. PG
2. TBHQ
3. DMT
4. BHA
5. BHT

throughout our analytical process

(Emily C. Luk, Schering Plough, USA)

App ID 1072

SAW PALMETO BERRY, P-BROMOPHENACYL ESTERS


Column:
Dimensions:
Order No.:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

Luna 3 C8(2)
150 x 4.6mm
00F-4248-E0
Acetonitrile/Water (87:13)
1.5mL/min
25C
UV @ 254nm
1. Capric acid
2. Lauric acid
3. Linolenic acid
4. Myristic acid
5. Linoleic acid
6. Palmitic acid
7. Oleic acid
8. Stearic acid

Column:
Dimensions:
Order No:
Mobile Phase:

Luna 5 C18(2)
150 x 4.6mm
00F-4252-E0
Acetonitrile/Water,
pH 3.1 w/1mM H3PO4 (70:30)
1.3mL/min
75C
UV @ 210nm
1. Cyclosporin A
2. Cyclosporin D

App ID 1108

CYCLOSPORIN - IMMUNOSUPPRESSANTS

Flow Rate:
Temperature:
Detection:
Sample:

App ID 1293

ACETAMINOPHEN, USP METHOD


Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Detection:
Temperature:
Sample:

Luna 5 C18(2)
150 x 4.6mm
00F-4252-E0
Water/Methanol/Acetic
Acid (69:28:3)
1.5mL/min
UV @ 275nm
45C
1. Acetaminophen
2. Caffeine
3. Benzoic Acid

App ID 1076

CAPSAICIN
Column:
Dimensions:
Order No:
Mobile Phase:
Gradient:
Flow Rate:
Detection:
Sample:

Luna 5 C18(2)
150 x 4.6mm
00F-4252-E0
A: Acetonitrile/Water (35:65)
B: Acetonitrile/Water (60:40)
100% A in 1 min to
100% B in 29 min
1.5mL/min
UV @ 227nm
1. Nordihydrocapsaicin
2. Capsaicin
3. Dihydrocapsaicin
4. Homocapsaicin

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Luna Phenyl-Hexyl
Engineered for Stability
The patented Luna Phenyl-Hexyl is a reproducible, extremely stable
phenyl phase. Most other phenyl phases use a short propyl (3
carbon) linker, which limits the phase stability. This patented phase
uses a hexyl (6 carbon) linker to attach the phenyl group to the
silica surface. The result:
Highly reproducible phenyl phase
Dual selectivity of both phenyl phase
and a short alkyl phase
(such as a C8)
Excellent retention of amine and polar
aromatic compounds
1.5 to 10 pH stability for over 6000 hours

ANTIBACTERIALS: COMPARISON OF PHENYL COLUMNS

Dimensions: 150 x 4.6 mm


Mobile Phase: A: 20mM KH2PO4, pH 2.5
B: Acetonitrile
Gradient: A/B (80:20) to A/B (75:25) in 5 min,
then to A/B (55:45) in 15 min
Flow Rate: 1.0mL/min
Detection: UV @ 254nm
Temperature: Ambient
Sample: 1. Carbadox
2. Thiamphenicol
3. Furazolidone
4. Oxolinic Acid
5. Sulfadimethoxine
6. Sulfaquinoxaline
7. Nalidixic Acid
8. Piromidic Acid

Agilent Technologies Zorbax 5 Phenyl

App ID 10823

Agilent Technologies Zorbax 5 SB-Phenyl

App ID 10821

6
7

3
2

CONDITIONS SAME FOR ALL COLUMNS4

App ID 1201

Phenomenex LUNA 5 Phenyl-Hexyl

Waters Spherisorb 5 Phenyl

10

15

20 min

App ID 10822

5,6

7
3
1, 2

4
8

2.5

18 x Phenomenex x Tel. 310.212.0555 x Fax 310.328.7768 x Web www.phenomenex.com

5.0

7.5

10.0

12.5

15.0

17.5

20.0 min

cut page

App ID 2575

COUGH AND COLD-USP METHOD


Column:
Dimensions:
Order No:
Mobile Phase:

Flow Rate:
Temperature:
Detection:
Sample:

App ID 1200

CHLOROBENZENES
Column:
Dimensions:
Order No:
Mobile Phase:
Gradient:
Flow Rate:
Temperature:
Detection:
Sample:

FOOD ADDITIVES
Column:
Dimensions:
Order No:
Mobile Phase:

Luna 5 Phenyl-Hexyl
150 x 4.6 mm
00F-4257-E0
Methanol/Water with 0.1% H3PO4
and 0.1% Heptane Sulfonate/
Acetonitrile with 0.1% H3PO4
(35:55:10)
2.05mL/min
22C
UV @ 214 nm
1. Acetaminophen
2. Pseudoephedrine
3. Chlorphenaramine
4. Dextromethorphan

Luna 5 Phenyl-Hexyl
150 x 4.6 mm
00F-4257-E0
A: Water
B: Acetonitrile
A/B (60:40) to A/B
(45:55) in 10 min
1mL/min
22C
UV @ 254nm
1. Chlorobenzene
2. 1,2-Dichlorobenzene
3. 1,4-Dichlorobenzene
4. 1,2,3-Trichlorobenzene
5. 1,3,5-Trichlorobenzene
6. 1,2,3,4-Tetrachlorobenzene

Gradient:
Flow Rate:
Temperature:
Injection:
Detection:
Sample:

COUGH AND COLD MEDICINE


Column:
Dimensions:
Order No:
Mobile Phase:
Gradient:
Flow Rate:
Temperature:
Injection:
Detection:
Sample:

App ID 1199
Luna 5 Phenyl-Hexyl
150 x 4.6mm
00F-4257-E0
A: 50mM KH2PO4 + 0.1%H3PO4
B: Acetonitrile
A/B (75:25) to A/B (25:75) in 18min,
hold at A/B (25:75) for 12min
1mL/min
22C
20L
UV @ 230nm
1. Saccharin
2. p-Hydroxybenzoic acid
3. Sorbic acid
4. p-Hydroxbenzoic acid methyl ester
5. Dehydroacetic acid
6. p-Toluic acid
7. p-Hydroxybenzoic acid ethyl ester
8. n-Propyl p-hydroxybenzoate

App ID 2572
Luna 3 Phenyl-Hexyl
75 x 4.6mm
00C-4256-EO
A: Acetonitrile
B: Methanol/20 mM KH2PO4 (80:20) pH 9.0
A/B (0:100) to A/B (80:20) in 5min
1mL/min
22C
20L
UV @ 214nm
1. p-Aminophenol
2. Benzoic acid
3. Acetaminophen
4. Pseudoephedrine
5. Butyl paraben
6. Chlorphenaramine
7. Diphenhydramine
8. Dextromethorphan

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Luna Cyano (CN)


Proven Reproducibility
Luna CN was developed to provide reproducibile chromatography
from run-to-run, column-to-column and batch-to-batch. Lunas
high-purity silica provides a ridged and dense column bed that
allows for improved CN bonding techniques to make a stable CN
phase. The result:
One of the most stable CN columns under both reversed
phase or normal phase conditions.
Reproducible from run-to-run, column-to-column,
batch-to-batch.
pH stable from 1.5 to 7.0

Column-to-Column Reproducibility

Batch-to-Batch Reproducibility

App ID 3464

App ID 3466
Column: Luna 5 CN
Dimensions: 150 x 4.6mm
Mobile Phase: A=Hexane, B=Methylene
chloride/Methanol(80/20),
A/B (95:5)
Flow Rate: 1.0mL/min
Injection: 5L
Detection: UV @ 254nm
Temperature: Ambient
Sample: 1. Dimethyl phthalate
2. Diethyl phthalate
3. Dibutyl phthalate
4. Dioctyl phthalate

Column: Luna 5 CN
Dimensions: 150 x 4.6mm
Mobile Phase: A=Hexane, B=Methylene chloride/
Methanol(80:20), A/B (80:20)
Flow Rate: 2.0mL/min
Detection: UV @ 254nm
Injection: 1.0 L
Temperature: Ambient
Sample: 1. Hydrocortisone
2. Prednisone
3. Cortisone
4. Hydrocortisone Acetate

App ID 3471

PHTHALATE ESTERS: A COMPARSION OF CN COLUMNS


Phenomenex LUNA 5 CN

20 x Phenomenex x Tel. 310.212.0555 x Fax 310.328.7768 x Web www.phenomenex.com

Waters Spherisorb 5 CN

Agilent Technologies
Zorbax 5 SB-CN

CONDITIONS SAME FOR ALL COLUMNS

Dimensions: 150 x 4.6 mm


Mobile Phase: A=Hexane, B= Methylene
chloride/Methanol (80:20), A/B (99:1)
Flow Rate: 1.0mL/min
Detection: UV @ 254nm
Temperature: Ambient
Sample: 1. Di-n-octyl phthalate
2. Bis (2-Ethylhexyl) phthalate
3. Butylbenzyl phthalate
4. Di-n-butyl phthalate
5. Diethyl phthalate
6. Dimethyl phthalate

...it made it easier for us to separate a known impurity


which we could not do before with another column...
(Steve Gottra, R.W. Johnson, USA)

App ID 3473

EXPLOSIVES
Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

App ID 3469

ORGANOCHLORINE PESTICIDES
Column:
Dimensions:
Order No:
Mobile Phase:
Gradient:
Flow Rate:
Temperature:
Detection:
Sample:

Luna 5 CN
150 x 4.6 mm
00F-4255-E0
A: Hexane
B: Methylene chloride/methanol
A/B (100:0) to A/B (99:1) in 1.5min,
A/B (99:1) to A/B (95:5) in 3.0min
1mL/min
22C
UV @ 230 nm
1. Aldrin
2. Heptachlor
3. DDT
4. Endrin
5. Dieldrin

App ID 3486

RHODAMINE DYES
Column:
Dimensions:
Order No:
Mobile Phase:

Luna 5 CN
150 x 4.6 mm
00F-4255-E0
Water and Methanol (65:12 v/v)/
Acetonitrile (80:20)
1mL/min
22C
UV @ 254nm
1. Nitrobenzene
2. m-Dinitrobenzene
3. 1,3,5-Trinitrobenzene
4. HMX
5. 4-Nitrotoluene
6. 2,4,6-Trinitrotoluene
7. Tetryl

Flow Rate:
Temperature:
Detection:
Sample:

Luna 5 CN
150 x 4.6 mm
00F-4255-E0
20mM Potassium phosphate,
pH 3.0 and Methanol/
Acetonitrile (50:50)
1mL/min
22C
UV @ 210 nm
1. Rhodamine 123
2. Rhodamine B
3. Rhodamine 6G

App ID 9300

BENZALKONIUM CHLORIDE
Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

Luna 5 CN
150 x 4.6 mm
00F-4255-E0
100mM Sodium acetate pH 5.0/
Acetonitrile (40:60)
2mL/min
22C
UV @ 254 nm
1. Benzalkonium chloride-C12
2. Benzalkonium chloride-C14

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Luna seems very versatile


Luna Amino (NH2)
Developed for Ruggedness
Luna Amino was developed to provide improved amino column
lifetime. Column life for most amino columns can be problematic
as the amino bonding easily strips off the silica. Luna Amino,
however, shows good bonded phase stability under both normal
and reversed phase modes and across a pH range of 1.5 to 11.0.
Such a broad pH range indicates the bonded phase ruggedness
and the density of the bonded phase coverage. The result:
Long lifetimes and low phase bleed for more
reproducible methods
Excellent retention of simple sugars, complex
sugars, sugar alcohols by reverse phase conditions
and hydrogen bonding compounds under
normal phase conditions
pH stable from 1.5 to 11.0
Stable in 100% aqueous mobile phases

SIMPLE SUGARS

STABILITY AT PH 11.0

STABILITY IN 100% AQUEOUS MOBILE PHASE

Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

Column:
Dimensions:
Order No.:
Mobile Phase:
Flow Rate:
Detector:
Temperature:
Conditions:

22 x Phenomenex x Tel. 310.212.0555 x Fax 310.328.7768 x Web www.phenomenex.com

Luna 5 NH2
250 x 4.6mm
00G-4378-E0
20mM Potassium Phosphate Buffer
pH 2.7/Acetonitrile (95:5)
1.5mL/min
UV@254nm
Ambient
Continuously flushed at 1.0mL/min
using 20mM Potassium Phosphate
Buffer pH 11.0/Acetonitrile (95:5)
between injections

APP ID 14285
Luna 5 NH2
250 x 4.6 mm
00G-4378-E0
Acetonitrile/Water
(80:20)
3mL/min
40C
RI
1. Xylose
2. Fructose
3. Glucose
4. Sucrose
5. Maltose
6. Melezitose
7. Raffinose

Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Detector:
Temperature:
Injection:
Conditions:

Luna 5 NH2
250 x 4.6mm
00G-4378-E0
20mM Potassium Phosphate
Buffer pH 2.7
1.5mL/min
UV@254nm
Ambient
2.5L
Continuously flushed at
1.0mL/min
using 100% 20mM
Potassium Phosphate Buffer
pH 2.7 between injections

& is a real asset to our column collection


(Amanda Baker, Parnell Laboratories, Australia)

NUCLEIC ACID BASES


Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

SUGAR ALCOHOLS
Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

APP ID 14302
Luna 5 NH2
150 x 4.6 mm
00F-4378-E0
Acetonitrile/Water
(80:20)
1mL/min
40C
UV @ 254 nm
1. Thymine
2. Uracil
3. Cytosine
4. Adenine
5. Guanosine

STEROIDS

APP ID 14289

COMPLEX SUGARS

Luna 5 NH2
250 x 4.6 mm
00G-4378-E0
Acetonitrile/Water
(85:15)
3mL/min
40C
RI
1. Glycerol
2. Pentaerythritol
3. Xylitol
4. Sorbitol
5. Inositol

Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

Column:
Dimensions:
Order No:
Mobile Phase:
Flow Rate:
Temperature:
Detection:
Sample:

APP ID 14299
Luna 5 NH2
250 x 4.6 mm
00G-4378-E0
Hexane/Ethanol (85:15)
2mL/min
22C
UV @ 240 nm
1. 11-Ketoprogesterone
2. 11-Hydroxyprogesterone
3. Cortisone Acetate
4. Prednisolone 21-Acetate
5. Cortisone
6. Prednisolone

APP ID 14309
Luna 5 NH2
250 x 4.6 mm
00G-4378-E0
Acetonitrile/Water (65:35)
3mL/min
40C
RI
1. Glucose
2. Maltose
3. Maltotriose
4. Maltotetraose
5. Maltopentaose
6. Maltohexaose
7. Maltoheptaose

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Both robust
LC/MS & Combinatorial Chemistry
Luna materials are highly recommended for LC/MS and highthroughput screening. Due to Lunas stability from pH 1.5 to 10, a
broad range of volatile, MS-compatible mobile phase modifiers
are available for LC/MS applications. High loading capacity is
possible due to high pore volume and optimum pore size distribution
of Luna silica. Our proprietary bonding procedure produces uniform
bonded phase coverage excellent for rapid mobile phase reequilibration. The result:
Sharp peak shape
High loading capacity
Low phase bleed
Rapid re-equilibration

PEAK SYMMETRY IN MS-COMPATIBLE BUFFERS

LC/MS ANALYSIS OF ANTICONVULSANTS

Column:
Dimensions:
Order No.:
Mobile Phase:
Flow Rate:
Mass Spec:
Scan Range:
Sample:

APP ID 2565
Luna 5 C8(2)
150 x 2.0mm
00F-4249-B0
10mM Ammonium
Acetate/Acetonitrile,
(20:80)
200L/min
Sciex API 100,
electrospray
50-3,000
1. Primidone
2. Carbamazepine
3. Phenytoin

PERFORMANCE IN MS-COMPATIBLE BUFFER


Columns:
Dimensions:
Order No.:
Mobile Phase:

Luna 5 C8(2)
100 x 4.6mm
00D-4249-E0
Methanol and 10mM Buffer
Ratio adjusted so that k in
10mM Ammonium formate
pH 3.5/Methanol was approx.
4.0 then maintained same
ratio and changed buffer
Flow Rate: 1.5mL/min
Sample: (all injected individually)
Neutral compounds
Acidic compounds
Basic compounds

APP ID 11080
0.1% Formic acid

APP ID 11081
0.1% Acetic acid

APP ID 11082
0.1% TFA

APP ID 11083
10mM Ammonium
acetate pH 5.0

24 x Phenomenex x Tel. 310.212.0555 x Fax 310.328.7768 x Web www.phenomenex.com

Column:
Dimensions:
Order No.:
Mobile Phase:
Gradient:
Flow Rate:
Detection:
Temperature:
Injection:
Sample:

Luna 3 C18(2)
50 x 4.6mm
00B-4251-E0
A = Water w/indicated modifier
B = Acetonitrile w/indicated
modifier
A/B (95:5) to A/B (5:95) in 6 min
2.0mL/min
UV @ 254nm
25C
5L
1. Acetaminophen (90ng)
2. Propranolol (2g)
3. Imipramine (200ng)
4. Naproxen (400ng)
5. Valerophenone (150ng)

& reliable

Luna has become the column of choice for our routine LC/MS service, typically
(Debra Manning, CPB Twyford, United Kingdom)
providing over 1400 analyses per week.
Capillary Columns
High Efficiency and High Sensitivity
Luna capillary columns have the same characteristics as their
respective analytical-size columns but provide added sensitivity
for microliter sample volumes. The incredible smoothness and
sphericity of Luna silica results in a high efficiency capillary-LC
column with a stable column bed that yield long column life. Our
high-purity silica combined with proprietary bonding techniques
produce a dense bonded phase shielding against silanol
interactions. With little secondary silanol interaction, Luna capillaryLC columns yield terrific peak shape, an essential criteria for mass
limited samples.
The result:
High efficiency 0.30, 0.50 and 0.70mm ID
glass-lined columns
Exceptional sensitivity for low volume and mass
limited samples
Consistent performance under LC/MS compatible
and universal gradient mobile phase conditions
1/8 inch (3mm) OD tube for robust, durable columns

b-LACTOGLOBULIN TRYPTIC DIGEST

Column:
Dimensions:
Order No.:
Mobile Phase:
Gradient:
Flow Rate:
Detection:
Temperature:
Sample:

b-LACTOGLOBULIN (OXIDIZED) TRYPTIC DIGEST

Column:
Dimensions:
Order No.:
Mobile Phase:
Gradient:
Flow Rate:
Detection:
Temperature:
Sample:

APP ID 14339
Luna 3 C18(2)
150 x 0.3mm
00F-4251-AC
A = 0.01% TFA in water
B = 0.008% TFA in Acetonirtrile
A/B (95:5) to A/B (55:45) in
60min, A/B (55:45) to A/B (95:5)
in 1sec, hold for 10min
5L/min
UV @ 210nm
40C
b-Lactoglobulin
tryptic digest

APP ID 14340
Luna 3 C18(2)
150 x 0.3mm
00F-4251-AC
A = 0.01% TFA in water
B = 0.008% TFA in Acetonitrile
A/B (95:5) to A/B (55:45) in
60min, A/B (55:45) to A/B (95:5)
in 1sec, hold for 10min
5L/min
UV @ 210nm
40C
b-Lactoglobulin (oxidized)
tryptic digest

Phenomenex x Tel. 310.212.0555 x Fax 310.328.7768 x Web www.phenomenex.com x 25

The performance observed

for the analytical columns extends


Preparative LC
Less Material Required
Luna materials have extremely low particle densities meaning less
material is required to pack a given volume. For large-scale
preparative systems, such as production or process methods, this
can have an enormous impact on long-term operating costs. For
example, packing a 50L column would require up to 20% less Luna
media than the leading competitive media.

Luna 10u C18(2) 100A


Leading 10u C18 100A

Exceptional Lifetimes
The extraordinary silica and bonding technology at the heart of
Luna provides exceptional lifetime. The rigid silica resists crushing,
improving the chromatographic performance over the life of the
column.

Column Bulk
Volume Material

0.3 kg

Quick, Direct Scale-Up


Luna preparative media can provide nearly the same
chromatographic behavior as a Luna analytical column. Method
scale-up is simple. Luna 3 C18(2) material used in the analytical
separation can be switched to Luna 15 C18(2) material to obtain
similar separation and resolution with minimal changes in the
separation method. Furthermore, the same smooth silica surface,
high bonded phase coverage and pH stability available in analytical
Luna materials is inherently present in all preparative Luna materials.

0.5L

High Loadability
Luna packing materials provide high surface area silica with
optimized pore size and pore size distribution and a high pore
volume that allows extremely high loading capacity.

5L

0.4 kg

0.5 kg
1L

0.7 kg

2.7 kg
3.6 kg

10.9 kg
20L

14.6 kg

20% LESS
MATERIAL REQUIRED

27.2 kg
50 L

36.4 kg

26 x Phenomenex x Tel. 310.212.0555 x Fax 310.328.7768 x Web www.phenomenex.com

to the preparative scale HPLC also.


(Debra Manning, CPB Twyford, United Kingdom)

Bonded Phases
LUNA PHASE

Pore
Size
()

Surface
Area
(m2/g)

Carbon
Load
(%)

Bonded
Phase
Coverage
(mole/m2)

pH Stability

3,5,10,15

100

400

2.0 - 7.5

C5

5,10

100

440

12.5

7.85

1.5 - 10

C8

5,10

100

440

14.75

5.50

1.5 - 10

C8(2)

3,5,10,
10-PREP,15

100

400

13.5

5.50

1.5 - 10

C18

5,10

100

440

19.0

3.00

1.5 - 10

C18(2)

3,5,10,
10-PREP,15

100

400

17.5

3.00

1.5 - 10

Phenyl-Hexyl

3,5,10,
10-PREP,15

100

400

17.5

4.00

1.5 - 10

CN

3,5,10

100

400

7.0

3.80

1.5 - 7.0

NH2

3,5,10

100

400

9.5

5.80

1.5 - 11

Silica(2)

Particle
Size ()

DESCRIPTION

APPLICATION

C5
Reversed-phase chemistry that offers greater hydrolytic stability
and an alternative selectivity over the classic C4 phase.
C8(2)
Optimized for maximum efficiency, peak shape and resolution.
Significantly improved performance over traditional C8 phases
due to high surface coverage. Slightly lower carbon load than
original Luna C8.
C8
Original C8 phase. Highly retentive; optimized for long lifetime at
extremely high pH levels (pH >10.0).
C18(2)
Optimized for maximum efficiency, excellent peak shape and
resolution; slightly lower carbon load than original Luna C18.

Highly hydrophobic compounds. Good alternative


to C8 or C18 when less retention is desired.

Great starting point for methods development of


pharmaceuticals, nucleotides and polar compounds.
Excellent for highly aqueous and LC/MS applications.

A workhorse for extremely high pH applications


(pH >10.0), or when a more retentive C8 is desired.

A C18 phase for virtually all HPLC applications.


Range of particle sizes offers amazing versatility for
capillary-LC/MS, to prep and process scale applications.

C18
Original LUNA C18 phase. Maximum retention, optimized for long
lifetime at extremely high pH levels (pH >10.0)

A workhorse for extremely high pH applications


(>10.0), or when maximum retention is desired.

Phenyl-Hexyl
Novel (patented) phenyl phase which employs a hexyl linker, as
opposed to the traditional propyl chain. Stable under all conditions
as well as alternative selectivity to most phenyl phases.

Unique selectivity for very polar and aromatic


compounds when used in methanol containing mobile
phases.

CN
One of the best performing CN phases available. Can be used in
reversed- or normal-phase modes. Based on same 99.999% pure
silica as all Luna phases.

Excellent for improving the retention of polar


compounds. Extremely rapid equilibration makes it
ideal for rapid screening and gradient applications.

NH2
Possibly the most rugged and reproducible NH2 phase for reversed
or normal phase separations. Stable from pH 1.5 to 11.0 and
under 100% aqueous conditions.

Excellent for reversed-phase analysis of sugars, sugar


alcohols and anionic compounds or for hydrogen
bonding compounds under normal phase.

Phenomenex x Tel. 310.212.0555 x Fax 310.328.7768 x Web www.phenomenex.com x 27

If Luna does not provide at least


an equivalent separation as compared to a competing column of the
similar particle size, phase and
dimensions, send in your comparative data within 45 days and keep the
Luna column for FREE.

ORDERING INFORMATION

3 and 5 Capillary
Columns (mm)
Phases
3 C8(2)
3 C18(2)
5 C8(2)
5 C18(2)

50 x 0.30

150 x 0.30

250 x 0.30

50 x 0.50

150 x 0.50

250 x 0.50

50 x 0.70

150 x 0.70

250 x 0.70

00B-4248-AC
00B-4251-AC
00B-4249-AC
00B-4252-AC

00F-4248-AC
00F-4251-AC
00F-4249-AC
00F-4252-AC

00G-4248-AC
00G-4251-AC
00G-4249-AC
00G-4252-AC

00B-4248-AF
00B-4251-AF
00B-4249-AF
00B-4252-AF

00F-4248-AF
00F-4251-AF
00F-4249-AF
00F-4252-AF

00G-4248-AF
00G-4251-AF
00G-4249-AF
00G-4252-AF

00B-4248-AG
00B-4251-AG
00B-4249-AG
00B-4252-AG

00F-4248-AG
00F-4251-AG
00F-4249-AG
00F-4252-AG

00G-4248-AG
00G-4251-AG
00G-4249-AG
00G-4252-AG

150 x 1.0

30 x 2.0

50 x 2.0

100 x 2.0

150 x 2.0

*4 x 2.0mm

**4 x 3.0mm

00A-4162-B0
00A-4248-B0
00A-4251-B0
00A-4254-B0
00A-4377-B0
00A-4256-B0

00B-4162-B0
00B-4248-B0
00B-4251-B0
00B-4254-B0
00B-4377-B0
00B-4256-B0

00D-4162-B0
00D-4248-B0
00D-4251-B0
00D-4254-B0
00D-4377-B0
00D-4256-B0

00F-4162-B0
00F-4248-B0
00F-4251-B0
00F-4254-B0
00F-4377-B0
00F-4256-B0

AJ0-4347
AJ0-4289
AJ0-4286
AJ0-4304
AJ0-4301
AJ0-4350

AJ0-4348
AJ0-4290
AJ0-4287
AJ0-4305
AJ0-4302
AJ0-4351

3 Microbore and Minibore

SecurityGuardCartridges 10/pk

Columns (mm)
Phases

30 x 1.0

50 x 1.0

Silica(2)

C8(2)
00A-4248-A0
00B-4248-A0
00F-4248-A0
C18(2)
00A-4251-A0
00B-4251-A0
00F-4251-A0
CN

00F-4377-A0
NH2
Phenyl-Hexyl
00A-4256-A0
00B-4256-A0

SecurityGuardCartridges require universal holder Order No.: KJO-4282

3 Analytical

SecurityGuardCartridges 10/pk

Columns (mm)
Phases

30 x 3.0

50 x 3.0

150 x 3.0

Silica(2)

00F-4162-Y0
C8(2)
00A-4248-Y0
00B-4248-Y0
00F-4248-Y0
C18(2)
00A-4251-Y0
00B-4251-Y0
00F-4251-Y0
CN

00B-4254-Y0
00F-4254-Y0

00B-4377-Y0
00F-4377-Y0
NH2
Phenyl-Hexyl

00B-4256-Y0
00F-4256-Y0
SecurityGuardCartridges require universal holder Order No.: KJO-4282

30 x 4.6

50 x 4.6

75 x 4.6

100 x 4.6

150 x 4.6

*4 x 2.0mm

**4 x 3.0mm

00A-4248-E0
00A-4251-E0
00A-4254-E0
00A-4377-E0
00A-4256-E0

00B-4162-E0
00B-4248-E0
00B-4251-E0
00B-4254-E0
00B-4377-E0
00B-4256-E0

00C-4248-E0
00C-4251-E0
00C-4254-E0
00C-4377-E0
00C-4256-E0

00D-4162-E0
00D-4248-E0
00D-4251-E0
00D-4254-E0
00D-4377-E0
00D-4256-E0

00F-4162-E0
00F-4248-E0
00F-4251-E0
00F-4254-E0
00F-4377-E0
00F-4256-E0

AJ0-4347
AJ0-4289
AJ0-4286
AJ0-4304
AJ0-4301
AJ0-4350

AJ0-4348
AJ0-4290
AJ0-4287
AJ0-4305
AJ0-4302
AJ0-4351

5 Microbore and Minibore

SecurityGuardCartridges 10/pk

Columns (mm)
Phases

50 x 1.0

150 x 1.0

250 x 1.0

Silica(2)

C5

C8

C8 (2)
00B-4249-A0
00F-4249-A0
00G-4249-A0
C18

C18 (2)
00B-4252-A0
00F-4252-A0
00G-4252-A0
CN

00F-4378-A0
00G-4378-A0
NH2
Phenyl-Hexyl
00B-4257-A0
00F-4257-A0

SecurityGuardCartridges require universal holder Order No.: KJO-4282


*for column ID 2.0-3.0 -**for column ID 3.2-8.0
28 x Phenomenex x Tel. 310.212.0555 x Fax 310.328.7768 x Web www.phenomenex.com

30 x 2.0

50 x 2.0

150 x 2.0

250 x 2.0

*4 x 2.0mm

**4 x 3.0mm

00A-4043-B0
00A-4040-B0
00A-4249-B0
00A-4041-B0
00A-4252-B0
00A-4255-B0
00A-4378-B0
00A-4257-B0

00B-4274-B0
00B-4043-B0
00B-4040-B0
00B-4249-B0
00B-4041-B0
00B-4252-B0
00B-4255-B0
00B-4378-B0
00B-4257-B0

00F-4274-B0
00F-4043-B0
00F-4040-B0
00F-4249-B0
00F-4041-B0
00F-4252-B0
00F-4255-B0
00F-4378-B0
00F-4257-B0

00G-4274-B0

00G-4040-B0
00G-4249-B0
00G-4041-B0
00G-4252-B0
00G-4255-B0
00G-4378-B0
00G-4257-B0

AJ0-4347
AJ0-4292
AJ0-4289
AJ0-4289
AJ0-4286
AJ0-4286
AJ0-4304
AJ0-4301
AJ0-4350

AJ0-4348
AJ0-4293
AJ0-4290
AJ0-4290
AJ0-4287
AJ0-4287
AJ0-4305
AJ0-4302
AJ0-4351

ORDERING INFORMATION

15 Preparative and Pilot Scale


Columns (mm)
Phases

250 x 4.6

250 x 21.2

250 x 30

250 x 50

250 x 100

50 x 21.2

50 x 50

Silica(2)
C8(2)
C18(2)
Phenyl-Hexyl

00G-4271-E0
00G-4272-E0
00G-4273-E0
00G-4286-E0

00G-4271-P0
00G-4272-P0
00G-4273-P0
00G-4286-P0

00G-4271-U0
00G-4272-U0
00G-4273-U0
00G-4286-U0

00G-4271-V0
00G-4272-V0
00G-4273-V0
00G-4286-V0

00G-4271-W0
00G-4272-W0
00G-4273-W0
00G-4286-W0

03B-4271-P0
03B-4272-P0
03B-4273-P0
03B-4286-P0

03B-4271-V0
03B-4272-V0
03B-4273-V0
03B-4286-V0

100g

1kg

5kg

10kg

50kg

100kg

Silica(2)
Bonded Silica

04G-4091

04K-4091

04L-4091

04M-4091

04N-4091

04P-4091

C5
C8
C8(2)
C18
C18(2)
CN
NH2
Phenyl-Hexyl

04G-4092
04G-4093
04G-4250
04G-4094
04G-4253
04G-4300
04G-4379

04K-4092
04K-4093
04K-4250
04K-4094
04K-4253
04K-4300
04K-4379

04L-4092
04L-4093
04L-4250
04L-4094
04L-4253
04L-4300
04L-4379

04M-4092
04M-4093
04M-4250
04M-4094
04M-4253
04M-4300
04M-4379

04N-4092
04N-4093
04N-4250
04N-4094
04N-4253
04N-4300
04N-4379

04P-4092
04P-4093
04P-4250
04P-4094
04P-4253
04P-4300
04P-4379

Phenyl-Hexyl

04G-4285

04K-4285

04L-4285

04M-4285

04N-4285

04P-4285

100g

1kg

5kg

10kg

50kg

100kg

Silica(2)
Bonded Silica

04G-4322

04K-4322

04L-4322

04M-4322

04N-4322

04P-4322

C8(2)
C18(2)
Phenyl-Hexyl

04G-4323
04G-4324

04K-4323
04K-4324

04L-4323
04L-4324

04M-4323
04M-4324

04N-4323
04N-4324

04P-4323
04P-4324

Phenyl-Hexyl

04G-4325

04K-4325

04L-4325

04M-4325

04N-4325

04P-4325

100g

1kg

5kg

10kg

50kg

100kg

Silica(2)
Bonded Silica

04G-4271

04K-4271

04L-4271

04M-4271

04N-4271

04P-4271

C8(2)
C18(2)
Phenyl-Hexyl

04G-4272
04G-4273

04K-4272
04K-4273

04L-4272
04L-4273

04M-4272
04M-4273

04N-4272
04N-4273

04P-4272
04P-4273

Phenyl-Hexyl

04G-4286

04K-4286

04L-4286

04M-4286

04N-4286

04P-4286

Guard(mm)

10 Bulk Packings
Phase

10
or
10-PREP?

10 vs. 10-PREP:
What is the difference?
The Luna 10 and 10-PREP differ by only
one factor: Particle size distribution. The
silica matrix and bonding chemistry are
identical, but the 10-PREP has a wider
particle size distribution to reduce cost.
Which should I choose?
Luna 10-PREP offers exceptional
performance, suitable for most applications, with excellent economy. Luna 10
is available when the absolute best preparative performance is critical. Extremely
narrow size distribution yields the highest
efficiency and maximum resolution.
Evaluation is the Best Test
All Luna materials are available in scout
columns. Contact your Phenomenex
representative to set up an evaluation.

10-PREP Bulk Packings


Phase

15 Bulk Packings
Phase

x Phenomenex x Tel. 310.212.0555 x Fax 310.328.7768 x Web www.phenomenex.com

ORDERING INFORMATION

Method Development Kits


Order No.

Description

KH0-4760

Luna Method Development Kit (5 columns)


1 each of 5 C18(2), C8(2), C5, CN, Phenyl-Hexyl Phases
Luna Rapid-MD Kit (3 columns)
1 each of 3 C18(2), C8(2) and Phenyl-Hexyl phases
Luna Selectivity Kit 1 (3 columns)
1 each of 5 C18(2), Phenyl-Hexyl, CN phases
Luna Selectivity Kit 2 (3 columns)
1 each of 5 C8(2), Phenyl-Hexyl, CN phases

KH0-4354
KHO-4761
KH0-4762

Dimensions(mm)

Unit

150 x 4.6

5/pk

50 x 4.6

3/pk

150 x 4.6

3/pk

150 x 4.6

3/pk

Dimensions (mm)

Unit

150 x 4.6
150 x 4.6
150 x 4.6
150 x 4.6
150 x 4.6

3/pk
3/pk
3/pk
3/pk
3/pk

Price

Method Validation Kits


Order No.

Description

KH0-4355
KH0-4356
KH0-4357
KH0-4358
KH0-4359

Luna 5 C18 Method Validation Kit


Luna 5 C18(2) Method Validation Kit
Luna 5 C8 Method Validation Kit
Luna 5 C8(2) Method Validation Kit
Luna 5 Phenyl-Hexyl Method Validation Kit

Price

ORDERING INFORMATION

Guard Cartridge System

LUNA Method
Development Kits
The Luna Method Development Kits
combine the incredible performance of
Luna HPLC columns with three different
selectivities. Both kits include a Luna
Phenyl-Hexyl (moderate polarity), a Luna
CN (polar), and your choice of a C8(2) or
C18(2) (non-polar). A wide range of
selectivies for virtually any separation
challenge.

LUNA Validation Kits


Three columns of the same phase from
three different batches provide an excellent
benchmark for system validation. Method
reprodicibility is easily and quickly
assessed, clearly demonstrating
chromatographic performance, validation
potential and long term compliance with
Luna Method Validation Kits.

Holder Assembly Kit

Order No.

Replacement Parts

Unit

AJ0-4283
AJ0-4285
AQ0-1389

PEEK Ferrules
Stacking Rings
PEEK (Biocompatible)
Fingertight fittings
SecurityGuard Wrenches

3/pk
2/pk
10/pk

AJ0-4284

Price

Order No.

Description

KJO-4282

Guard Cartridge Kit.


Includes: 1 cartridge
holder, 3 PEEK ferrules,
2 wrenches, 2 stacking
rings, 2 PEEK fingertight fittings

Price

2/pk

Cartridges
Cartridges for General Purpose/Pharmaceutical
Order No.

Material
Description

Dimensions
(mm)

Column ID

pH Stability

Unit

AJ0-4286
AJ0-4287
AJ0-4289
AJ0-4290
AJ0-4292
AJ0-4293
AJ0-4347
AJ0-4348
AJ0-4350
AJ0-4351
AJ0-4304
AJ0-4305
AS0-4301
AS0-4302

C18
C18
C8
C8
C5
C5
Silica
Silica
Phenyl
Phenyl
CN
CN
NH2
NH2

4 x 2.0
4 x 3.0
4 x 2.0
4 x 3.0
4 x 2.0
4 x 3.0
4 x 2.0
4 x 3.0
4 x 2.0
4 x 3.0
4 x 2.0
4 x 3.0
4 x 2.0
4 x 3.0

2.0 - 3.0 mm
3.1 - 8.0 mm
2.0 - 3.0 mm
3.1 - 8.0 mm
2.0 - 3.0 mm
3.1 - 8.0 mm
2.0 - 3.0 mm
3.1 - 8.0 mm
2.0 - 3.0 mm
3.1 - 8.0 mm
2.0 - 3.0 mm
3.1 - 8.0 mm
2.0 - 3.0mm
3.1 - 8.0mm

1.5 - 10
1.5 - 10
1.5 - 10
1.5 - 10
1.5 - 10
1.5 - 10

1.5 - 10
1.5 - 10
1.5 - 7
1.5 - 7
1.5 - 11
1.5 - 11

10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk
10/pk

Price

Phenomenex x Tel. 310.212.0555 x Fax 310.328.7768 x Web www.phenomenex.com x

ORDERING INFORMATION

5 Analytical
Columns (mm)
Phases

SecurityGuardCartridges 10/pk
30 x 3.0

50 x 3.0

150 x 3.0

Silica(2)

C5

00F-4043-Y0
C8

00F-4040-Y0
C8(2)
00A-4249-Y0
00B-4249-Y0
00F-4249-Y0
C18

00F-4041-Y0
C18(2)
00A-4252-Y0
00B-4252-Y0
00F-4252-Y0
CN

00B-4255-Y0
00F-4255-Y0

00B-4378-Y0
00F-4378-Y0
NH2
Phenyl-Hexyl
00A-4257-Y0
00B-4257-Y0
00F-4257-Y0
SecurityGuardCartridges require universal holder Order No.: KJO-4282

250 x 3.0

30 x 4.6

50 x 4.6

75 x 4.6

*4 x 2.0mm

**4 x 3.0mm

00G-4043-Y0
00G-4040-Y0
00G-4249-Y0
00G-4041-Y0
00G-4252-Y0
00G-4255-Y0
00G-4378-Y0
00G-4257-Y0

00A-4040-E0
00A-4249-E0
00A-4041-E0
00A-4252-E0
00A-4255-E0
00A-4378-E0
00A-4257-E0

00B-4274-E0
00B-4043-E0
00B-4040-E0
00B-4249-E0
00B-4041-E0
00B-4252-E0
00B-4255-E0
00B-4378-E0
00B-4257-E0

00C-4040-E0
00C-4249-E0
00C-4041-E0
00C-4252-E0
00C-4255-E0
00C-4378-E0
00C-4257-E0

AJ0-4347
AJ0-4292
AJ0-4289
AJ0-4289
AJ0-4286
AJ0-4286
AJ0-4304
AJ0-4301
AJ0-4350

AJ0-4348
AJ0-4293
AJ0-4290
AJ0-4290
AJ0-4287
AJ0-4287
AJ0-4305
AJ0-4302
AJ0-4351

250 x 10

250 x 15

250 x 21.2

250 x 30

Guard (mm)
50 x 10

00G-4274-N0
00G-4043-N0
00G-4040-N0
00G-4249-N0
00G-4041-N0
00G-4252-N0
00G-4255-N0
00G-4378-N0
00G-4257-N0

00G-4274-AK

00G-4249-AK

00G-4252-AK

00G-4257-AK

00G-4274-P0

00G-4249-P0

00G-4252-P0

00G-4257-P0

00G-4274-U0

00G-4249-U0

00G-4252-U0

00G-4257-U0

5 Analytical, Semi-Prep and Preparative


Columns (mm)
Phases

100 x 4.6

150 x 4.6

250 x 4.6

Silica(2)
00D-4274-E0
00F-4274-E0
00G-4274-E0
C5
00D-4043-E0
00F-4043-E0
00G-4043-E0
C8
00D-4040-E0
00F-4040-E0
00G-4040-E0
C8(2)
00D-4249-E0
00F-4249-E0
00G-4249-E0
C18
00D-4041-E0
00F-4041-E0
00G-4041-E0
C18(2)
00D-4252-E0
00F-4252-E0
00G-4252-E0
CN
00D-4255-E0
00F-4255-E0
00G-4255-E0
00D-4378-E0
00F-4378-E0
00G-4378-E0
NH2
Phenyl-Hexyl
00D-4257-E0
00F-4257-E0
00G-4257-E0
SecurityGuardCartridges require universal holder Order No.: KJO-4282

SecurityGuardCartridges 10/pk
*4 x 2.0mm

**4 x 3.0mm

03B-4274-N0

03B-4040-N0
03B-4249-N0
03B-4041-N0
03B-4252-N0
03B-4255-N0
03B-4378-N0
03B-4257-N0

AJ0-4347
AJ0-4292
AJ0-4289
AJ0-4289
AJ0-4286
AJ0-4286
AJ0-4304
AJ0-4301
AJ0-4350

AJ0-4348
AJ0-4293
AJ0-4290
AJ0-4290
AJ0-4287
AJ0-4287
AJ0-4305
AJ0-4302
AJ0-4351

50 x 50
03B-4091-V0
03B-4092-V0
03B-4093-V0
03B-4250-V0
03B-4094-V0
03B-4253-V0

03B-4285-V0

5u Columns for High-Throughput Purification


Columns (mm)
Phases
Silica(2)
C8(2)
C18(2)

50 x 21.2

100 x 21.2

150 x 21.2

50 x 30

75 x 30

100 x 30

00B-4274-P0
00B-4249-P0
00B-4252-P0

00D-4274-P0
00D-4249-P0
00D-4252-P0

00F-4274-P0
00F-4249-P0
00F-4252-P0

00B-4274-U0
00B-4249-U0
00B-4252-U0

00C-4274-U0
00C-4249-U0
00C-4252-U0

00D-4274-U0
00D-4249-U0
00D-4252-U0

10 Semi-Prep and Preparative


Columns (mm)
Phases

250 x 4.6

250 x 10

250 x 15

Silica(2)
00G-4091-E0
00G-4091-N0
00G-4091-AK
C5
00G-4092-E0
00G-4092-N0

C8
00G-4093-E0
00G-4093-N0

C8(2)
00G-4250-E0
00G-4250-N0
00G-4250-AK
C18
00G-4094-E0
00G-4094-N0

C18(2)
00G-4253-E0
00G-4253-N0
00G-4253-AK
00G-4379-E0
00G-4379-N0
00G-4379-AK
NH2
Phenyl-Hexyl
00G-4285-E0
00G-4285-N0
00G-4285-AK
SecurityGuardCartridges require universal holder Order No.: KJ0-4282
*for column ID 2.0-3.0 -**for column ID 3.2-8.0
x Phenomenex x Tel. 310.212.0555 x Fax 310.328.7768 x Web www.phenomenex.com

250 x 21.2

250 x 30

250 x 50

250 x 100

50 x 10

Guard (mm)
50 x 21.2

00G-4091-P0
00G-4092-P0
00G-4093-P0
00G-4250-P0
00G-4094-P0
00G-4253-P0
00G-4379-P0
00G-4285-P0

00G-4091-U0

00G-4250-U0

00G-4091-V0
00G-4092-V0
00G-4093-V0
00G-4250-V0
00G-4094-V0
00G-4253-V0
00G-4379-V0
00G-4285-V0

00G-4091-W0
00G-4092-W0
00G-4093-W0
00G-4250-W0
00G-4094-W0
00G-4253-W0
00G-4379-W0
00G-4285-W0

03B-4091-N0
03B-4092-N0
03B-4093-N0
03B-4250-N0
03B-4094-N0
03B-4253-N0

03B-4285-N0

03B-4091-P0
03B-4092-P0
03B-4093-P0
03B-4250-P0
03B-4094-P0
03B-4253-P0

03B-4285-P0

00G-4253-U0
00G-4379-U0
00G-4285-U0

SecurityGuardCartridges 10/pk
*4 x 2.0mm **4 x 3.0mm
AJ0-4347
AJ0-4292
AJ0-4289
AJ0-4289
AJ0-4286
AJ0-4286

AJ0-4350

AJ0-4348
AJ0-4293
AJ0-4290
AJ0-4290
AJ0-4287
AJ0-4287

AJ0-4351

2320 W. 205th St.


Torrance, CA 90501-1456
U.S.A.

LUNA

The Worlds #1 HPLC Column

Luna is available worldwide. For the distributor in your country contact Phenomenex USA,
International Department by telephone, fax or e-mail: international@phenomenex.com

U.S.A.
(310) 212-0555
(310) 328-7768

PUERTO RICO
(800) 541-HPLC
(310) 328-7768

CANADA
(800) 543-3681
(310) 328-7768

UNITED KINGDOM
01625-501367
01625-501796

GERMANY
06021-58830-0
06021-58830-11

NEW ZEALAND
09-4780951
09-4780952

AUSTRALIA
1800 553 929
1800 553 923

PEB20181INT-01

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