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LIST OF APPARATUS AND MATERIAL

Instruction :
each questions.

Please check the apparatus and material given for

Question 1
Bil

Apparatus and material


1. Test tube

Quantity
4

2. Test tube rack

3. Test tube holder

4. Stopwatch

5. 5 ml Syringe

6. Dropper

7. Water bath (water, beaker and


thermometer)
8. Solution A

1
5ml

9. Solution B

5ml

10. Solution C
11. 1 % pepsin

5ml
10 ml

Yes (v)/ no (x)

Question 1
[20 Marks]
Answer all questions
Enzymes are proteins which are found in human body to speed up the
biochemical reaction. They are sensitive to pH.
Example of enzyme is pepsin. Pepsin is found in the human stomach. It
hydrolyses albumen to polypeptides at a suitable pH.

The aim of this experiment is to investigate the effect of different


solution on the activity of pepsin.
You are provided with hydrochloric acid, sodium hydroxide solution
and distilled water.

Diagram 1

Based on Diagram 1, plan and carry out the experiment to


investigate the effect of different solution on the activity of pepsin.
Carry out this experiment by using the following steps.
1. Label three test tubes P, Q and R.
2. Put 5ml of albumen suspension into each test tube using a
syringe.
3. Add the following solutions into each test tube:
P: 1ml of solution A + 1 ml of pepsin solution
Q: 1 ml of solution B + 1 ml of pepsin solution

R: 1 ml of solution C + 1 ml of pepsin solution


4. Immerse all the test tubes in the water bath where the
temperature is maintained at 37oC for 20 minutes.
5. Observe the conditions of the mixtures at the beginning of the
experiment and again after 20 minutes.
6. Record your results.
Based on the experiment which has been carried out, answer the
following questions:
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a) Construct a table and record your reading.

COMMUNIC
A
TION

Solution
A
B
C

Condition of mixture
Clear
Cloudy
cloudy
(3m)

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CONTROL
ING
VARIABLE

b) State the following variables


Manipulated variable: types of solution
Responding variable: condition of the mixture
Constant variable: time/ temperature/ volume
(3m)

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OBSERVI
NG

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INFERRI
NG

c) (i) State the solution that hydrolyses albumen.


Solution A.
(1m)
c) (ii) Give reason for your answer in b (i).
Albumen is clear in solution A while cloudy in solution B & C. B
(1m)

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PREDICTI
NG

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MAKING
HYPOTH
ESIS

d) Predict the pH of the solution that you named in b (i)


pH 1-6/ low pH
(1m)
e) State the hypothesis
3

Mixture turns clear in solution A but not in solution B & C.


(3m)
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PREDICTI
NG
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INFERRI
NG

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DEFININ
G
OPERATI
ONALLY

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CLASSIFYI
NG

f) If the albumen is replaced with starch, state the observation.


The content will remain cloudy.

(1m)

g) Give a reason for your answer.


Starch is carbohydrate, pepsin cannot hydrolyse starch.
(2m)
i) Based on this experiment, state the operational definition of
enzyme.
Enzyme is the condition of the mixture in different type of
solution in 15 minutes. Condition of mixture depends on type
of solution.
(3m)
j) Based on this experiment, classify the list of apparatus &
materials given on page 1?
(2m)
Apparatus

Material

Question 2
[10 marks]
The population of garden snails in SMK Menglembu is estimated.
Observe the diagram below and answer the following questions.

MEASURIN
G AND
USING
NUMBER

1st capture
2nd capture
20
27
ai) In the space provided, count the number of garden snails
captured.
(2
mark)

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ii) Estimate the population of garden snails in SMK Menglembu.


mark)

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RELATIONS
HIP BETW.
SPACE &
TIME

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Estimated population = 20 x 27 = 90
6

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EXPERIME
NT
ING

bi) Give two characteristics of the ink that is used to mark the snails.
(2marks)
- Water proof
5

- Should not be toxic


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PREDICTI

ci) Instead of using black ink, a bright fluorescent ink is used to


mark the snails. State whether the estimated population of the
garden snail will increase or decrease.
Decrease
mark)

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INFERRI

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ii) Give a reason for your answer in ci).


(2 marks)
Bright colour attracts the predator easily. Thus, the predator eats
the prey.

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EXPERIME
NT
ING

d) Name the technique that can be used to estimate the population


of immobile organism.
Quadrat sampling technique.
mark)

(1