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ID #: 35093434

Sp2016
"Name (last, first):"
Lab Partner Name:

"Rajawat, Adityavikram"
LD

"Tables 1-5 are used to summarize data for each buffer or control. FOR ALL MEANS
, PH CHANGES, AND STANDARD DEVIATIONS, YOU MUST ENTER THE APPROPRIATE FORMULAS,
INCLUDING CORRECT CELL REFERENCES. (e.g. '=AVERAGE(B11:Mll)' is the average of t
he data values in cells B8 through G8, and '=B11-B17' is pH change for the first
sample in Table 1.) Do not include the quotation marks, but the equal sign (=)
must be included for all formulas. For more information, see Lab Report Instruct
ions in Lab Manual or Excel Instructions posted in UB learns."
Table 1: Water
Sample: Water
Your initials (enter during lab): --> LE & JL AT & RA MR & AD ZR & AC HG & JM
HC & RD EQ & TR SG & MS AW & BG LD & AR SH & JC
Drops HCl
pH
pH
pH
pH
pH
pH
pH
pH
pH
pH
pH
pH
Mean
0
7.17
6.86
7.10
7.45
7.28
6.70
5.90
7.40
7.44
6.74
7.25
7.03
5
6.70
6.46
6.18
6.64
6.83
6.22
4.56
6.76
6.99
5.15
4.11
6.05
10
6.27
6.11
4.90
5.50
6.46
5.90
2.76
6.24
6.68
3.34
3.24
5.22
15
5.90
5.81
3.41
3.96
6.16
5.62
2.65
5.62
6.42
3.09
2.94
4.69
20
5.23
5.35
3.12
3.61
5.83
5.34
2.55
4.15
6.16
2.99
2.80
4.28
Standard Deviation pH change
25
3.98
4.35
2.96
3.41
5.42
5.06
2.49
3.63
5.85
2.83
2.70
3.88
30
3.65
3.69
2.83
3.29
4.27
4.39
2.45
3.41
5.31
2.74
2.62
3.51
pH change*: --> 3.52
3.17
4.27
4.16
3.01
2.31
3.45
3.99
2.13
4.00
4.63
3.51
0.80
"<- e.g., if all columns are fil
led, enter '=STDEV(B18:M18)' without the quotation marks"
"*You must enter the formula, including cell references, to calculate the INITIA
L pH minus FINAL pH (e.g =B11-B17)."
Table 2: Phosphate Buffer
Sample: Phosphate buffer
Your initials (enter during lab): --> MR & AD SH & JC EQ & TR AT & RA HG & JM
AW & BG LD & AR RD & HC JL & LE ZR & AC SG & MS
Drops HCl
pH
pH
pH
pH
pH
pH
pH
pH
pH
pH
pH
pH
Mean
0
6.82
6.91
6.90
6.87
6.77
6.94
6.86
6.96
6.90
7.13
6.84
6.90
5
6.81
6.86
6.85
6.84
6.75
6.92
6.82
6.95
6.84
7.11
6.82
6.87
10
6.80
6.83
6.81
6.83
6.74
6.92
6.80
6.94
6.81
7.09
6.81
6.85
15
6.79
6.81
6.78
6.82
6.74
6.91
6.79
6.93
6.80
7.08
6.80
6.84
20
6.79
6.79
6.76
6.81
6.73
6.91
6.78
6.92
6.78
7.07
6.79
6.83
Standard Deviation pH change
25
6.78
6.78
6.74
6.80
6.73
6.91
6.77
6.91
6.77
6.99
6.79
6.82
30
6.77
6.77
6.73
6.80
6.72
6.90
6.76
6.90
6.76
7.03
6.78
6.81
pH change*: --> 0.05
0.14
0.17
0.07
0.05
0.04
0.10
0.06

0.14

0.10

0.06

0.09

0.04

Table 3: Bio Buffer 1


Bio Buffer 1: Beef Broth
Your initials (enter during lab): -->
Drops pH
pH
pH
pH
0
5.72
5.71
5.78
5.71
5
5.42
5.51
5.52
5.67
10
5.27
5.28
5.35
5.63
15
5.05
5.10
5.14
5.58
20
4.89
4.94
4.98
5.54
25
4.79
4.82
4.85
5.51
30
4.68
4.70
4.74
5.45
pH change*: --> 1.04
1.01
1.04

SH & JC EQ & TR LD & AR RD & HC


Mean
5.73
5.53
5.38
5.22
5.09
Standard Deviation pH change
4.99
4.89
0.26
0.84
0.39

Table 4: Bio Buffer 2


Bio Buffer 2: Yeast Extract
Initials (enter during lab): -->
Drops pH
pH
pH
pH
0
6.75
6.85
6.70
6.75
5
6.71
6.73
6.69
6.72
10
6.68
6.71
6.68
6.70
15
6.63
6.68
6.67
6.69
20
6.59
6.66
6.66
6.67
25
6.55
6.65
6.65
6.65
30
6.53
6.64
6.64
6.64
pH change*: --> 0.22
0.21
0.06

MR & AD LE & JL AT & RA SG & MS


Mean
6.76
6.71
6.69
6.67
6.65
Standard Deviation pH change
6.63
6.61
0.11
0.15
0.08

Table 5: Bio Buffer 3


Bio Buffer 3: Apple Juice
Initials (enter during lab): -->
Drops pH
pH
pH
pH
0
3.42
3.42
3.65
5
3.40
3.44
3.68
10
3.38
3.44
3.69
15
3.36
3.45
3.70
20
3.35
3.46
3.71
25
3.34
3.46
3.70
30
3.34
3.47
3.69
pH change*: --> 0.08
-0.05 -0.04

HG & JM AW & BG ZR & AC


Mean
3.50
3.51
3.50
3.50
3.51
Standard Deviation for pH change
3.50
3.50
0.00
0.07

Graph 1: Graph mean pH (dependent variable; y-axis) vs. Drops (independent vari
able; x-axis) for ALL 6 TABLES above using a straight marked scatter chart. (See
Lab Report Instructions for more information.)
"For Table 6, enter the correct cell references for each Mean and Stdev pH chang
e from Tables 1-6 to obtain the appropriate values (e.g. '=H15' should be entere
d for the Mean pH change for water, etc.)"
Table 6: REORGANIZED DATA
Solution
Mean pH change
Water
3.51
0.80
Phosphate Buffer
0.09
Bio buffer 1 name:
Beef Broth
Bio buffer 2 name:
Yeast Extract
Bio buffer 3 name:
Apple Juice

Stdev pH change
0.04
0.84
0.15
0.00

0.39
0.08
0.07

Graph 2: Graph Mean pH change for each of the biological buffers and the control
s using a Column (i.e. Clustered Column) chart. Remember to enter Stdev values f
or +/- error bars. (See Lab Report Instructions)

Questions
1. State a reasonable hypothesis for this experiment. (0.5 pt; Remember that a h
ypothesis is a statement)
The hypothesis for this experiment was that buffers help control change in pH va
lue.

2. What was the negative control for this experiment and what did it control fo
r? (0.5 pts)
The negative control in the experiment was HCL and it controlled the change in p
H value.

3. Was your hypothesis correct? Explain your answer. (0.5 pt)


Yes our hypothesis was correct as the negative control had an effect on our depe
ndent variable.

4. Which of the solutions - including controls- was the best overall buffer? (i
.e. smallest mean pH change)? (0.5 pt)
Bio buffer 3 (apple juice)was the best overall buffer
Tables 1-5 data and pH Means (1 pt):
Tables 1-5 pH change and Stdev (1 pt):
Graph 1: pH (2 pts):
Table 7 (1 pt):
Graph 2: pH change (1 pt):
Total for questions (2 pts):
TOTAL (OUT OF 8 POSSIBLE):

0.00