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DEL ROSARIO, Beatrice Camille G.

ZOO302 Assignment; Listing

July 20, 2014

Fixatives

Histological Stains

Immunohistochemical Stains

1.ExCell Plus

1.Periodic acid-Schiff reaction


(PAS)

1.Alpha-1-Antichymotrypsin

The ExCell plus fixative is an alternative


to the 10% neutral buffered formalin. It
firms the tissue, and is able to have effects
of penetration as quick as the 10%
formalin.

The PAS stain is able to stain up to


glycogen, mucus in cells, basement
membranes and brush borders of kidney
tubules and small or large reticular fibers
like collagen in connective tissues and
cartilages.

This is used in identifying atypical


fibroxanthomas in skin and malignant
fibrous histiocytomas in soft tissue.

2.Bouins 2000

2.Massons trichome

2.Chromogranin

The Bouins 200- fixative is a replacement


for the Bouins fluid. Asides from it being
a fixative it is also used as a mordant agent
as well.

The Massons trichome stain is often used


to stain connective tissue.

The chromogranin stain is used as a


panendocrine marker since it is majorly
expressed by neuroendocrine tumors.

3.Karnovskys Fixative

3.Van Gieson

The Karnovskys fixative is used for


Electron microscopy. This used to fix the
lung tissue in animal inhalation studies.

The Van Gieson stain is often used for


staining blood vessels and the skin.

3.Epithelial Membrane Antigen


(EMA)

4.B*5 Mercuric Chloride Fixative 4.Azan

The EMA is helpful in diagnosis of the


extramammary in Pagets disease, eccrine
neoplasms and sebaceous carcinoma.

4. Prostate Specific Antigen


(PSA)

This B*5 fixative is used as for


hematopoietic and lymphoid tissues such
as lymph nodes, and also for gastric
biopsies.

The Azan stain is used for staining


connective tissue and the epithelium.

5.Ultrum II Fixative

5.Giemsa

5. Vimentin

The Ultrim II fixative is used for routine


and special stains.

The Giesma stain is usually used to stain


bone marrow and blood smears.

The Vimentin is used as a generalized


marker for mesenchymal tumors.

The PSA is an antibody used to recognize


primary and metastatic prostalic
neoplasms.

References
Rolls, G. (2012). Fixation and fixatives popular fixative solutions. Leica Biosystems.
Retrieved from http://www.leicabiosystems.com/pathologyleaders/fixation-and-fixatives4-popular-fixative-solutions/
Histological stains other than H&E. The Histological Guide. Retrieved July 20, 2014
from http://histology.leeds.ac.uk/what-is-histology/histological_stains.php
Special stains and immunochemistry. Central Coast Pathology. Retrieved July 20,
2014 from http://www.ccpathology.com/special_stains_immunohistochemistry.asp
Tissue fixatives from American MasterTech. Retrieved July 20, 2014 from
http://www.americanmastertech.com/tissue_fixatives.htm