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You are required to plan out an investigation about the effect of temperature on the
reaction rate of enzyme activity. The activity of the enzyme will be determined by
time taken for hydrolysis to be completed.
Your experimental planning should include the following:
 Aim of investigation
 Statement of hypothesis
 Variables
 List of apparatus and materials
 Techique used
 Experimental procedure or method
 Presentation of data
 Conclusion
Write a report of the investigation/plan

(17 marks)

Aim: To study the effects of temperature on the activity of salivary amylase.
Problem statement: What are the effects of temperature on the activity of salivary
Hypothesis: The optimum temperature for salivary amylase is 37 ◦C.
Manipulated variable: Temperature
Responding variable: Rate of reaction
Fixed variables: volume and concentration of starch suspension, volume and
concentration of saliva suspension, pH of starch and saliva suspension.
Apparatus: Beakers, test tube, tripod stand, wire gauze, Bunsen burner, thermometer,
dropper, glass rod, syringes, stopwatch, spotting tile and test tube rack.
Materials: 1% starch suspension, saliva, iodine solution, ice cubes and distilled water
Technique: Recording the time taken for the complete hydrolysis of starch. The
presence of starch is indicated by the iodine test.
1. Rinse the mouth with distilled water.


Record the time taken for the complete hydrolysis of starch in the table. Label two test tube P and Q. Plot a graph of rate of reaction (1/t) against temperature (°C) Result: Temperature bath (°C) of water Time taken for complete Rate of hydrolysis of starch. Take o drop of suspension from test tube Q every one minute and test with iodine solution on the spotting tile. 8. 9. Put 4 ml of starch suspension in test tube P and 1 ml of saliva suspension in test tube Q. 37°C. (When the iodine solution does not turn blue anymore) 12. Pour the starch suspension from test tube P into the saliva suspension in the test tube Q. 50°C. The hypothesis is accepted. t (minute-1) (minutes) reaction 0 20 37 50 60 Conclusion: The rate of reaction is highest at the temperature 37°C. 5.2. Repeat step 4 to 9 for temperature 20°C. 10. 2 1/t . 7. Start the stopwatch immediately. 37°C. 60°C. 6. Prepared the saliva suspension by splitting into a small beaker and diluting the saliva with an amount of distilled water equal to the amount of saliva. 50°C. Keep the test tube Q in the water bath throughout the experiment. 20°C. Prepare five water bath at the following temperature : 0°C. 13. Place both test tube into the first water bath (at 0°C) for 5 minutes. 60°C. 4. Place one drop of iodine solution into each groove of spotting tile. 3. 11.