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Nitroglycerin

Molecular formula: C3H5N3O9
Molecular weight: 227.1
CAS Registry No.: 55-63-0

SAMPLE
Matrix: blood
Sample preparation: 3 mL Plasma + 12 mL dichloromethane: ethyl acetate 1:1, shake
mechanically at 250 cycles/min for 5 min, centrifuge at 550 g at 4° for 5 min. Remove the
organic phase and evaporate it to about 20 |xL under a stream of nitrogen at room temperature, inject.
HPLCVARIABLES
Column: 250 X 4 10 |xm Zorbax NH2
Mobile phase: n-Hexane: MeOH 95:5
Flow rate: 5
Injection volume: 20
Detector: Thermal energy analyzer, Thermo Electron Corp. Model 502A, furnace temp
575°, argon 15 mL/min, oxygen 25 mL/min, MeOH/dry ice slush bath
CHROMATOGRAM
Retention time: 5.0
Internal standard: nitroglycerin
OTHER SUBSTANCES
Extracted: isosorbide dinitrate, isosorbide mononitrate
KEYWORDS
plasma; nitroglycerin is IS
REFERENCE
Maddock, J.; Lewis, P.A.; Woodward, A.; Massey, RR.; Kennedy, S. Determination of isosorbide dinitrate
and its mononitrate metabolites in human plasma by high-performance liquid chromatography-thermal energy analysis. J.Chromatogr., 1983, 272, 129-136

SAMPLE
Matrix: formulations
Sample preparation: Inject directly.
HPLCVARIABLES
Column: 300 X 3.9 10 jxm pJBondapak C18
Mobile phase: MeCN:MeOH:water 6:6:13
Column temperature: 35
Flow rate: 2.8
Detector: UV 210
CHROMATOGRAM
Retention time: 2.8
KEYWORDS
injections; stability-indicating

Hosp.. Jarvi.Pharm.Ther. 2561-2563 SAMPLE Matrix: formulations HPLCVARIABLES Column: C18 Mobile phase: MeOH: water 50:50 Flow rate: 1. saline REFERENCE Martens. filter (0.. 47. 203-207 SAMPLE Matrix: formulations Sample preparation: Dilute with saline.45 |xm). 16. agitate with a stirring rod and sonicate. Y. glass. Yau. Stability of nitroglycerin as nitroglycerin concentrate for injection stored in plastic syringes. If the sample clumps when the buffer is added. RL.7 ammonium acetate buffer:water 55:10:35. 1990. Am. V.J. Gardner..REFERENCE Driver. 50.. de Goede.) .Pharm. add 10 mL MeOH. HPLCVARIABLES Column: 250 X 4.J. Sorption of various drugs in polyvinyl chloride.N. 1991. A..8 OTHER SUBSTANCES Simultaneous: isosorbide dinitrate KEYWORDS injections.3 Detector: UV 220 CHROMATOGRAM Internal standard: isosorbide dinitrate KEYWORDS injections.Pharm. Am. B..6 Lichrosorb 10 RP 8 Mobile phase: MeOH: water 50:50 Flow rate: 2 Injection volume: 10 Detector: UV 214 CHROMATOGRAM Retention time: 5. Das Gupta.Hosp. RN. make up to 100 mL with buffer. inject a 10 jxL aliquot. and polyethylene-lined infusion containers. RS.C. S. H.J.. nitroglycerin and sodium nitroprusside in disposable plastic syringes. (Buffer was MeOH:200 mM pH 4.J. Stabilities of dobutamine. dopamine.. inject a 20 JXL aliquot. 369-373 SAMPLE Matrix: formulations Sample preparation: Weigh out an amount of finely powdered tablets or capsules equivalent to about 25 mg of drug.. 1993. Gratzer. J. 5% dextrose. Add 50 mL buffer. E. van Loenen. shake for 30 min.Clin. stability-indicating REFERENCE Pramar.

HPLCVARIABLES Column: 300 X 3.Chromatogr. 55.Z. 1985. isosorbide mononitrate. saccharin KEYWORDS tablets..J. mouth rinses REFERENCE Noonan. mix. 184-187 SAMPLE Matrix: formulations Sample preparation: 1 mL Solution + 300 |xL isosorbide dinitrate solution. R. Determination of isosorbide dinitrate in pharmaceutical products by HPLC. Thompson. RK.ScL. 574-578 SAMPLE Matrix: formulations HPLCVARIABLES Column: 150 X 4.HPLCVARIABLES Guard column: 50 X 6. R.67 KEYWORDS tablets.6 5 |xm Ultrasphere ODS Mobile phase: MeOH: 200 mM pH 4.6 5 p.7 ammonium acetate buffer . pentaerythritol tetranitrate.D. Incomplete and delayed bioavailability of sublingual nitroglycerin.9 10 |xm jxBondapak phenyl Mobile phase: MeCN: THF: water 26:10:64 Flow rate: 2 Detector: UV 218 CHROMATOGRAM Internal standard: isosorbide dinitrate KEYWORDS injections.m C8 (Altex) Mobile phase: MeCN: water 40:60 Flow rate: 2.CardioL... J. Am.P. saline . Snell.4 25-37 |xm Whatman Co-Pell ODS Column: 250 X 4.water 55:10:35 Flow rate: 1 Injection volume: 20 Detector: UV 254 CHROMATOGRAM Retention time: 8 Internal standard: nitroglycerin OTHER SUBSTANCES Simultaneous: isosorbide dinitrate. Benet. M. inject an aliquot. 26. L. 1988. 5% dextrose. capsules. nitroglycerin is IS REFERENCE Carlson.

ScL.9% sodium chloride injection. 481-483 .Scl. D... J. HPLC VARIABLES Column: 300 X 3. 1985..E. 1303-1304 SAMPLE Matrix: formulations Sample preparation: Dilute with water. K.J..M. Am. and erythrityl tetranitrate in various tablet forms. pentaerythritol tetranitrate KEYWORDS tablets. J. inject an aliquot. Rapid and accurate stability-indicating assay for nitroglycerin. pentaerythritol tetranitrate. Gragg.. CS.Pharm. J. R. A. M.. nitroglycerin is IS REFERENCE Olsen.S.9 10 |xm jxBondapak C18 Mobile phase: MeOH: water 40:60 Flow rate: 1 Injection volume: 20 Detector: UV 220 CHROMATOGRAM Retention time: 14 Internal standard: nitroglycerin OTHER SUBSTANCES Simultaneous: erythrityl tetranitrate. 42.H. 361-362 SAMPLE Matrix: formulations Sample preparation: Powder tablets.Pharm. M. filter. Carter.F.6 ixBondapak C18/Corasil Column: 300 X 3. 73. H. isosorbide dinitrate. weigh out a portion equivalent to 3 mg erythrityl tetranitrate. Scroggins.. shake mechanically for 30 min. Soliman.REFERENCE Thompson. 1984.Pharm. 68. add to 10 mL 75 jJig/mL nitroglycerin in MeOH. High-performance liquid chromatographic determination of the nitrate esters isosorbide dinitrate. 1979. Amann. sonicate for 2 min. Smith.9 ixBondapack phenyl Mobile phase: MeCN: THF: water 26:10:64 Flow rate: 2 Injection volume: 10-40 Detector: UV 218 CHROMATOGRAM Retention time: 10 Internal standard: isosorbide dinitrate (6) KEYWORDS inj ections. inject an aliquot HPLCVARIABLES Guard column: 40 X 4.Hosp. stability-indicating REFERENCE Baaske. Stability of nitroglycerin and dobutamine in 5% dextrose and 0.

6 5 |xm Supelcosil LC-8 Mobile phase: MeOH: water 50:50 Flow rate: 1..R. dilute to 200 mL with MeOH. 673-676 SAMPLE Matrix: solutions HPLCVARIABLES Column: 33 X 4. M. HPLCVARIABLES Column: 250 X 4.5 Detector: UV 210 . 1990. Liquid chromatographic determination of pentaerythritol tetranitrate in Pharmaceuticals: collaborative study.m C18 (Perkin-Elmer) Mobile phase: MeCN: MeOH: water 24:24:52 Flow rate: 3. Pharm.6 3 u.6 5 |xm Ultrasphere ODS C18 Mobile phase: MeCN: water 65:35 Flow rate: 1 Injection volume: 20 Detector: UV 230 CHROMATOGRAM Retention time: 6 Internal standard: nitroglycerin OTHER SUBSTANCES Simultaneous: pentaerythritol tetranitrate KEYWORDS collaborative study.. 7. M.5-5 REFERENCE Roberts. let undissolved lactose settle. shake mechanically for 30 min.Assoc. nitroglycerin is IS REFERENCE Carlson. Mueller. inject a 20 |xL aliquot.Off. filter through paper. 693-697 SAMPLE Matrix: solutions HPLCVARIABLES Column: 150 X 4. K.Chem. sonicate for 5 min.SAMPLE Matrix: solutions Sample preparation: Add 10 g 10% nitroglycerin solution in lactose to 125 mL MeOH.Anal. Comparisons of in vitro nitroglycerin (TNG) flux across yucatan pig.. 1990.5 Detector: UV 210 CHROMATOGRAM Retention time: 4.Res. hairless mouse. 73. Dilute 5 mL to 250 mL with mobile phase. and human skins. J.E.

. extracted isosorbide dinitrate.. 1983.. capsules. Carter. plasma.Chromatogr. G. Karnatz. Koszegine Szalai. M.U.Pharm..Chem. or pentaerythritol tetranitrate. 55. Validation of high-performance liquid chromatographic methods for analysis of sustained-release preparations containing nitroglycerin.. T. isosorbide mononitrate. Determination of vasodilators and their metabolites in plasma by liquid chromatography with a nitrosyl-specific detector.. [High performance liquid chromatographic assay and determination of the even distribution of active ingredients in nitroglycerin and isosorbide dinitrate tablets].Sci. 2603-2620 [simultaneous pentaerythritol tetranitrate. 1983.. pentaerythritol tetranitrate.. Anal. L. P.M. R.Pharm.D. LOD 0. 124-126 Yu. 55. L.N.. D. SPE. 1988. Goff. 72. Thompson.Chromatogr. Paal. J. 445-449 • • • ANNOTATED BIBLIOGRAPHY Carlson. N. E.CHROMATOGRAM Retention time: 0. tablets. Determination of pentaerythritol tetranitrate in Pharmaceuticals by high performance liquid chromatography. J. J. nitroglycerin is IS] Torok. 29-32 [TEA detection.. 72. W.Hung. J.E.1 ng] . isosorbide dinitrate.. Rapid high-performance liquid chromatographic procedure for nitroglycerin and its degradation products.6 Limit of quantitation: 1 |xg/mL OTHER SUBSTANCES Simultaneous: degradation products REFERENCE Severin. High-performance liquid chromatographic assay for partially nitrated glycerins in nitroglycerin.. 154-162 Baaske. A. Papas. Keseru. 320.N.C. 194-196 Gelber. 1985. H.. Ada Pharm. 11. 1983. 1985. J.Sci.Liq.