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FACULTY OF APPLIED SCIENCES

BIO 150
METABOLISM AND CELL DIVISION
(SEM NOVEMBER 2014-APRIL 2015)
LABORATORY REPORT
EXPERIMENT 7:CELL REPRODUCTION II
Date of Experiment:11.02.2015
Date of Submission:12.022015

INTRODUCTON
Meiosis is the singularly important biological event that not only maintains a constant
chromosomes number for most species of plant or animals, but also provides a means of genetic
variable because of crossing over and the subsequent exchange of genetic material. In meiosis,
immature or primordinal germ cells undergo a reduction in the diploid number of chromosomes
characteristic for the species and become mature gametes.
In this study, meiosis will be studied as it occurs in the development o nature pollen
grains the flowering plants. This pollen grains give rise to male gametes, which fuse within an
egg produce a zygote.

OBJECTIVES
1. To observe and identify the stages of meiosis in Lilys anther.
2. To observe and identify the stages of meiosis from a given slide.

APPARATUS

Microscope
Slide of lilium root tip mitosis
Slide of lilium anther early prophase
Slide of lilium root tip mitosis
Slide of lilium root tip
Slide of lilum anther first divisions
Slide of lilium anther late prophase

PROCEDURE

1. 1 cm of apical was cut of from the lilium


2. The lilium was placed on the watch glass containing acetic orcein stain and HCL in the
approximately proportions of the ten parts of stain to one parts of acid.
3. The watch glass was warmed, but not boiled, for five minutes on the spirit lamp. The acid
helps the tissue to macerated so that the stain can be easily absorbed.
4. 3 mm of the lilium was cut and the part was discarded furthest from the apex.
5. Two or three drops of acetic orcein added to the lilium.
6. The lilium was broke with a needle so as to spread it out as thinly as posibles without
interfering too much with arrangement of the cells.
7. The cover slips was put on, covered with filter paper and squashed gently.
8. The slide was warmed on the spirit lamp for about ten secinds to intensify the staining.
The slide should be warmed but not to hot to touched.
9. The stages in meiosis was examained.
10. A labeled diagram for each stage was provided.
11. There was a simplified notes on the changes that occur in every stages in meiosis besides
the labeled diagram.

OBSRVATIONS
DIAGRAM
Prophase II

NOTES
New spindle apparatus is formed on each of
the two cells from telophase I. the stillduplicated chromosomes stretch out and then

recondense. Spindle microtubules from each


pole attach to the kinetochores of each of the
sister chromatids.
Metaphase II
The duplicated chromosomes are aligned along
the equator by the spindle complex in each of
the two cells.

Anaphase II
Centromeres of sister chromatids are separated
from

each

other

and

the

now

single

chromosomes are pulled to the poles of the


two cells.

Telophase II
Each new nucleus formed has half the number
of the original chromosomes and eacg nucleus
has

one

of

each

type

of

homologous

chromosome. Cytokinesis results in a total of


four new cells, the haploid products.
QUESTIONS
1. Where else you can find this process in Lily?
Anther.
2. How do you identify the meiotic division that occurs in the lilium anther?
The meiotic divisions is half the number of chromosomes as the parent. Four haploid
microscope will produce in each germ cell in the male organs or anther which undergoes

meiosis in the lily. Six rows of ovules in the lily ovulary, can produre egg cells by
meiosis. Meiosis I and Meiosis II are the two consecutive divisions in meiosis. The two
members of each pair of chromosomes separated in first meiotic divisions, the identical
chromatids of each chromosomes are separated in the second meiotic divisions.
3. Give the significance of meiotic process in organisms.
The ovule and ovary are given in variation in genetic.
4. Give the difference between the meiotic and mitotic process that occur in Lily.
Meiotic Process
Mitotic Process
There are two cell division, the first and the The cell divide only once.
second meiotic divisions.
Chromatids of two homologous chromosomes The two chromosome do not exchange
exchange segments during crossing over.
segments during prophase.
There is no centromeric division uring Divisions of the centromers take place during
anaphase I. Centromers divide only during anaphase.
anaphase II
Occurs in germ cells
Spindle fiber do not reappear at telophase I.

Occurs in the somatic cells of the body


Spindle fibers disappear completely
telophase.

in