Principal Investigator/Program Director (Last, first, middle

):

BIOGRAPHICAL SKETCH
NAME POSITION

Terence A. Partridge

Professor of Experimental Pathology & Head of Muscle Cell Biology Group
EDUCATION/TRAINING
DEGREE (if applicable) YEAR(s) FIELD OF STUDY

INSTITUTION AND LOCATION

University College London, London, UK University College London, London, UK

B.Sc. Ph.D.

1962 1970

Zoology Zoology (Embryology)

A. Positions and Honors POSITIONS AND EMPLOYMENT 1965 – 1966 Research Assistant – Museum Nationale d’Histoire Naturelle, Paris 1967 – 1970 Lecturer in Cell Biology – University of Glasgow 1970 – 1975 Research Fellow - Department of Experimental Pathology, Charing Cross Hospital Medical School,London. 1975 – 1980 Lecturer, Department of Experimental Pathology, Charing Cross Hospital Medical School, London 1978 – 1989 Senior Lecturer, Department of Experimental Pathology, Charing Cross Hospital Medical School, London 1989 – 1992 Reader in Experimental Pathology & Head of Muscle Cell Biology Group, MRC Clinical Sciences Centre, Imperial College School of Medicine 1993 – 1994 Professor of Experimental Pathology & Head of Muscle Cell Biology Group, MRC Clinical Sciences Centre, Imperial College School of Medicine 1994 – Present Professor of Experimental Pathology & Head of Muscle Cell Biology Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, HONORS 2002 - 2003 Member of National Center for Research Resources (NCRR), the National Institutes of Health (NIH). NCRR Human Embryonic Stem Ccll Special Emphasis Panel 2002 Elected to Fellowship of Academy of Medical Sciences 2004 - 2006 Chair Blaise Pascal - Fellowship awarded by the Region of the Ile de France B. Selected peer-reviewed publications (in chronological order). (Of 128 publications). Blaveri, K., Heslop, L., Yu, D.S., Rosenblatt, J.D., Gross, J.G. Partridge, T.A. & Morgan, J.E. (2000) Patterns of repair of dystrophic mouse muscle: studies on isolated fibres. Developmental Dynamics, 216, 244-256. Lu, Q.L., Morris, G.E., Wilton, S.D., Ly, T., Artem’yeva, O.V., Strong, P., Partridge, T.A. (2000) Massive idiosyncratic exon skipping corrects the nonsense mutation in dystrophic mouse muscle and produces functional revertant fibres by clonal expansion. J. Cell Biol. 148,985-995. Heslop, L. Morgan, J.E. and Partridge, T.A. (2000) Evidence for a myogenic stem cell that is exhausted in dystrophic muscle. J. Cell Science 113, 2299-2308. Morrison, J., Lu, Q.L., Pastoret, C., Partridge, T., Bou-Gharios, G. (2000) T-cell-dependent fibrosis in the mdx dystrophic mouse. Lab.Invest. 80, 881-891. Pagel, C.N., Morgan, J.E., Gross, J.G., Partridge, T.A. (2000). Thymic myoid cells as a source of cells for myoblast transfer. Cell Transplantation, 9: 531-538. Cooper, R. N.; Irintchev, A.; Di Santo, J. P.; Zweyer, M.; Morgan, J. E.; Partridge, T. A.; Butler-Browne, G. S.; Mouly, V., Wernig, A. (2001) A new immunodeficient mouse model for human myoblast transplantation. Human Gene Therapy; 12: 823-831. Beauchamp, J.R., Heslop, L., Yu, D.S.W., Tajbakhsh, S., Kelly, R.G.‡ Buckingham, M.E. Partridge, T.A., Zammit P.S. (2000) Expression of CD34 and Myf5 defines the majority of quiescent adult skeletal muscle satellite cells. J. Cell Biology 151: 1221-1233. Mann, C.J., Honeyman, K., Chen, A.J., Ly, T., Lloyd, F., Fletcher, S., Morgan, J.E., Partridge, T.A., Wilton, S.D. (2001). Antisense-induced exon skipping and synthesis of dystrophin in the mdx mouse. Proceedings of the National Academy of Sciences, U.S.A. 98: 42-47.
PHS 398 (REV. 5/01)
Number pages consecutively at the bottom throughout the application. Do not use suffixes such as 3a, 3b.

Biographical Sketch Format Page

Principal Investigator/Program Director (Last, first, middle):

Heslop, L., Beauchamp, J.R., Tajbakhsh, S., Buckingham, M.E., Partridge, T.A., Zammit, P.S. (2001) Transplanted primary myoblasts can give rise to functional satellite cells as identified using the Myf5nlacZ/+ mouse. Human Gene Therapy, 8: 778-783. Shefer, G., Partridge, T.A., Heslop, L., Gross, J.G., Oron, U. Halevy, O. (2002) Low-energy laser irradiation promotes the survival and cell-cycle entry of skeletal muscle satellite cells. Journal of Cell Science, 115: 1461-1469. De Val, S., Ponticos, M., Antoniv, T.T., Wells, D.J., Abraham, D., Partridge, T., Bou-Gharios, G. (2002). Indentification of the key regions within the mouse pro- 2(I) Collagen gene far upstream enhancer. Journal of Biological Chemistry, 277: 9286-9292. Morgan, J.E., Pagel, C.N., Beauchamp, J.R., Gross, J.G., Fassati, A., . Thrasher, A.J., Di Santo, J.P., Abraham, D.J., Fisher, I.B, Shiwen, X., Partridge T.A. (2002). Myogenic cell proliferation and generation of a reversible tumorogenic phenotype are triggered by pre-irradiation of the recipient site. Journal of Cell Biology.157:693-702. Zammit, P. S.; Heslop, L.; Hudon, V.; Rosenblatt, J. D.; Tajbakhsh, S.; Buckingham, M. E.; Beauchamp, J. R., and Partridge, T. A. (2002) Kinetics of myoblast proliferation show that resident satellite cells are competent to fully regenerate skeletal muscle fibers. Experimental Cell Research 15; 281: 39-49. Wells, K. E.; Torelli, S.; Lu, Q.; Brown, S. C.; Partridge, T.; Muntoni, F., and Wells, D. J. (2003) Relocalization of neuronal nitric oxide synthase (nNOS) as a marker for complete restoration of the dystrophin associated protein complex in skeletal muscle. Neuromuscular Disorders. 13: 21-31. Lu, Q-L, Bou-Gharios, G, Partridge, TA, (2003) Non-viral gene delivery in skeletal muscle: a protein factory. Gene Therapy.; 10:131-142 Lu, Q-L, Liang, H-D, Partridge, T, Blomley, MJK (2003) Microbubble ultrasound improves the efficiency of gene transduction in skeletal muscle in vivo with reduced tissue damage. Gene Therapy. 10: 396405 Lu, Q-L., Mann C. J., Lou, F., Bou-Gharios, G., Morris, G. E., Xue, S., Fletcher, S., Partridge, T. A. & Wilton, S. D. Functional amounts of dystrophin produced by skipping the mutated exon in the mdx dystrophic mouse. Nature Medicine 9, 1009 – 1014 ReimannJ, Brimah K, Schröder R, Wernig A, Beauchamp JR, Partridge TA. (2004). Pax7 distribution in human skeletal muscle biopsies and myogenic tissue cultures. Cell Tissue Res. 315;233-243 Murtuza, B, Suzuki, K., Bou-Gharios, G., Beauchamp J. R., Smolenski , R. T., Partridge, T. A., Yacoub M. H. Transplantation of myogenic precursors overexpressing interleukin-1 receptor antagonist modulates adverse remodeling in infarcted murine myocardium: PNAS:101;4216–4221 Cousins J.C., Woodward K. J.,. Gross J.G .,. Partridge T. A., Morgan. J.E. Regeneration of skeletal muscle from transplanted immortalised myoblasts is oligoclonal. J. Cell Sci. in press. Ponticos, M., Abraham, D., Alexakis, C Lu, ., Q-L., Black C,, Partridge T. Bou-Gharios G. Col1a2 enhancer regulates collagen activity during development and in adult tissue repair. Matrix Biology. (In press) Ponticos, M., Partridge T., Black1, C. M.. Abraham D. J., Bou-Gharios G. Regulation of collagen type I in vascular smooth muscle cells by competition between Nkx2.5 and deltaEF1/ZEB1. Mol Cell Biol, (in press) Zammit, P.S. ,Golding, J.P., Nagata, Y., Hudon, V., Partridge, T.A., Beauchamp, J.R. (2004) Muscle satellite cells adopt divergent fates: a mechanism for self-renewal? Journal of Cell Biology (in press). Peter S. Zammit†, Jaime J. Carvajal*, Jon P. Golding, Jennifer E. Morgan, Dennis Summerbell*, Terence A. Partridge, Peter W. J. Rigby* and Jonathan R. Beauchamp Myf5 expression in satellite cells and muscle spindles of adult muscle is controlled by separate genetic elements. Developmental Biology, (in press) C. Research Support ONGOING RESEARCH: Program Grant from MRC 2000- 2005 Cell Biology of Skeletal muscle

The objective of this program is to integrate studies of cellular functions in the maintenance and repair of skeletal muscle in vivo and in vitro so as to generate a coherent picture of the mechanims involved.
PHS 398 (REV. 5/01)
Number pages consecutively at the bottom throughout the application. Do not use suffixes such as 3a, 3b.

Biographical Sketch Format Page

Principal Investigator/Program Director (Last, first, middle):

RESEARCH COMPLETED DURING THE LAST 3 YEARS: European Community Network Grant Framework 5 1999-2002 Stem cells and precursor cells of skeletal muscle

This funding was specifically aimed at generating collaborative networks with other European laboratories so as to facilitate collaborations on the study of myogenic stem cells. Muscular Dystrophy Association 2001-4 Development and characterization of new markers for satellite cells

This MDA funded project was aimed at finding new reagents to permit the identification and characterization of the skeletal muscle satellite cell. We have identified a number of new markers and have used them to demonstrate the behavioural heterogeneity of satellite cells. Aktion Benni & Co 2002-4 Endogenous mechanisms of fibrosis in mdx mouse muscle

This project is aimed at determining which cells in skeletal muscle are responsible for the fibrosis that occurs in chronically injured muscle and the control elements of the collagen 1 gene that are responsible for the excessive production of scar tissue in such muscles. European Community Network Grant 1999-2003 Effects of aging on skeletal muscle satellite cells

This European funding was directed at fostering cooperation between a number of laboratories working on various aspects of aging in human and mouse skeletal muscle

Muscular Dystrophy Campaign. (with J. Morgan & P.Zammit

2004 - 2005

Characterization of the endogenous satellite stem cell subpopulation

This is one year of funding to establish a new technique for analyzing myogenicity of satellite cells by transplanting them in very small numbers (5-10) on single isolated muscle fibres. We find that under these conditions, satellite cells behave in a more ‘stem cell-like’ way than when they are separated from the muscle fibre Association Francaise contre les Myopathies 2004 - 2005 Characterization of the myogenic properties of the satellite cell

This funding is specifically to foster the use of the single fibre transplantation technique for analysis of myogenic cell behaviour using a number of transgenic markers that are available in mouse colonies in laboratories at the Genethon and the Pasteur Institute. Chair Internationale de Recherche Blaise Pascal de l’Etat et de la Region d’Ile-de-France, 2004 - 2006 Investigation of the diversity of satellite phenotypes and functions

PHS 398 (REV. 5/01)
Number pages consecutively at the bottom throughout the application. Do not use suffixes such as 3a, 3b.

Biographical Sketch Format Page

Principal Investigator/Program Director (Last, first, middle):

This is a prestigious award of financial support to conduct work of collaboration for a total duration of 1 year spread over a 2 year period. It is envisaged as involving the best Parisian laboratories (Genethon and Institut Pasteur) and the home laboratory ( in this case my MRC laboratory during 2005 and the Children’s Hospital during 2006. The objective is to bring together the specialist techniques of both sets of laboratories to gain previously unobtainable information on the nature of the variety of cells that are grouped together under the name of satellite cell and to determine the roles of these cell types in muscle repair after various types of injury.

PHS 398 (REV. 5/01)
Number pages consecutively at the bottom throughout the application. Do not use suffixes such as 3a, 3b.

Biographical Sketch Format Page