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DIPLOMA IN MEDICAL LABORATORY TECHNOLOGY

(DMLT) COURSE CURRICULUM
GENERAL INFORMATION
1. The Duration of Diploma Course of Medical Laboratory Technology (DMLT) is two years.
2. The minimum educational qualification for selection of trainees for the Diploma Course of
Medical Laboratory Technology is +2 Science with Biology as one of the subject.
3. Total marks of the DMLT Course is 1000.
4. Minimum pass mark of the trainees if 50% in Theory, 50% in Oral & 50% in Practical.
5. 1st Class mark is 60% in Theory, Practical & Oral in aggregate respectively.
6. Less than 40% either in Theory or in Practical or in Oral in any paper will be treated as
unsuccessful (Fail).

Computer Science+Statistics(Theory+ Practical) – 20hrs+10hrs 4.First Total Marks – 200 Theory . Physiology (Theory + Practical) . Medicine 20 . Medicine. Comm.140 + Practical 60 Theory Paper I Theory Paper II Practical Anatomy 35 Physiology 35 Comm. Anatomy (Theory+ Practical) – 20hrs+10hrs 2. Pharmacology (Theory) – 20hrs EXAM .20hrs+10hrs 3.4 Months Subject 16 weeks (110hrs) 1. Medicine.PART-1 BASIC COURSE ON MEDICAL LABORATORY TECHNOLOGY Duration . Computer Science + Statistics 35 Pharmacology 35 Anatomy 20 Physiology 20 Comm.

common planes & Anatomical terms. Bronchi Lungs and Pleura Digestive System.. 8. Surface Anatomy. Head & Neck. & Pelvis. Central & Peripheral nervous system.4 months Marks . Thymus & Tonsils Respiratory System. 7. 3. Thorax. Blood Vessels.Alimentary canal (different parts)-Liver. 2. -Taste & 01 factory sensations. Practical – 10hrs Marks . Trachea. Pancreases Peritoneum Urogenital System. 5. Important Nerve. General Sensibilities like touch.Eye & Vision-Ears.Brain & Spinal Cord.Different parts of urinary system -Different parts of Male & Female genital -System (Internal & External Genitalia) Special Senses& General Sensibilities.Axial and appendicular bones -Joints &movements Skin.Anatomical position. 6. 9. Total period . 10. Gall Bladder.Autonomic Nervous System.Abd. 11.Cranial & Spinal Nervous.E. Histology -Typical animal cell (Structure & Function) -4 primary tissues (Classification & function) Skeletal System .20 .System -Spleen. Lymphatic & R. temperature.Nose. -Different branches of Anatomy. Regional Anatomy (Only Demonstration)– Extremities. Larynx. pain. Important Blood Vessels. 1. Fascia and Muscles & Tendons Circulatory System –Heart. Hearing & Equilibrium. 4. Pharynx.SECTION-I ANATOMY Theory – 20 hrs. Important Muscles for Injection.35 Introduction to the subject .

External and internal respiration.Cerebrospinal Fluid. Endocrine-glands-Definition of endocrine gland. Function of ovary-formation of ova. Formation. Anticoagulants. Composition & Function. Names of the endocrine gland and the hormone secreted by them.normal counts & function. Only brief outline of the subjects to be given. composition of gastric juice. How inspiration. Pancreatic Juice. . Reparatory System -Name of structures involved in respirations and their function.Description of blood cells . Reproductive System-Name of primary and accessory organs in male and female.Functions of Glomerulus and tubules. compositions of Urine.20 Note: The teaching of Anatomy & Physiology should be coordinated so that structure and function of different parts of human body are correlated.Function of Skin.Steps of congratulation. Tidal volume. menstrual cycle. Bile and Digestion of food by different Enzymes. Absorption and Defecation.Composition and general function of blood. Practical – 10hrs Marks . Skin. expiration are brought about Transport of O2 and CO2 in the blood. Hypoxia. Excretory System-Functions of Kidney. Definition of respiratory rate. actions of ovarian hormone. Importance of blood groups composition & function of lymph. 1- 2- 34- 56- Mark – 35 Blood. vital capacity.Major actions of such Hormones. Digestive System-Composition and functions of saliva. mastication and deglutition. Nephron .SECTION-II PHYSIOLOGY Total Period – 4 months Theory – 20 hrs. normal& abnormal.Functions of Testes-Spermatogenesis and secretions of testosterone. Functions of stomach. Name of secondary sexual characters in male and female. Fertilization Vasectomy and tubectomy.

concept of Pharmacokinetics. . bile and other body fluids (Special emphasis). Route of administration. Total period .Anti-Bacterial Drugs.4 months Mark-35 1. Drugs interfering in different Pathological tests. Drugs for the diseases of fundamental System GI System.Blood. 2.Respiratory System.SECTION-III PHARMACOLOGY Theory – 20 hrs. Blood Coagulation. excretion of drugs in stool.Cardiovascular System.Endocrine System and autacoids. Chemotherapeutic Agents Anti-Viral including AIDs. Drug nomenclature. General Pharmacology Drug. Measurement of Drug levels in different body fluids and significance.Autonomic System. Drugs for diseases of integrating systems of body Central Nervous System.Anthelmintics.Drugs affecting the Urine and renal functions. 6. 3. 4. Anti-Protozoan Drugs. Thrombosis. 7. disinfectants. different types of anti-coagula (Special emphasis). Pharmaco-dynamics and Adverse during action. Hepatitis.Anti-Fungal Drugs. Antiseptic. 5.Anti-Cancer Drugs.

Computer Basics: Importance. Computer memory. History. Animal foods including milk Food-borne diseases of Public Health importance. Rats & Rodents. Fats and oils. Frequency Distribution Tables DIAGRAMS : Bar Diagrams. Part B Mark-15 STATISTICS-GENERAL TABULATIONS : Simple Tables. Histogram. Assessment of Nutritional status. Flies. 2. Part C Mark-5 COMPUTER 1. Roots and tubers. Input –output Devices. 2. external storage devices. Pulses. Fleas. Median. Anatomy of Computer. Food and Nutrition: Collection of different food samples :Cereals. Vegetables. Mode MEASURES OF DISPESION : Normal Curve. Internet 3. Networking. Computer and Communication. Purification Bio-Medical Waste Management Sanitation in Public Health 3.4 months Part A Mark-15 1.20 . Water Sources: Types. Lice. Processing Units and outline of Data Processing. Computer Generation. Line Diagram Pie Diagram STATISTICAL AVERAGES : Mean. Range. Identification and Public Health Importance of arthropods (Entomology): Mosquitoes. Hardware. Total period . Software Basic functioning of Computers. Standard Deviation Standard Error. TESTS OF SIGNIFICANCE : 't' Test. Use of computer in Radio-diagnosis/Pathology Laboratory Practical – 10hrs Marks . Types of Computer.SECTION-IV COMMUNITY MEDICINE (SPM) Theory – 20 hrs.

Plasma protein. Overview of Iron. Iodine. Calcium. Molarily. indicators.400. Radiosolips. factors regulating. institution 2 clinical application) 4. RSA. 5. Enzyms (Classification. Chemistry of TOTAL MARKS DURATION 150 10 MONTHS 150 10 MONTHS 100 10 MONTHS a) Carbohydrates including proctiglycom b) Fat c) Prosthesis & Amino acid 2.10 MONTHS PAPER PAPER-I PAPER-II PAPER-III SUBJECT MARKS PATHOLOGY THEORY 100 IMMUNOHEMATOLOGY BLOOD BANKING HEMATOLOGY PRACTICAL 30 ORAL 20 MICROBIOLOGY THEORY 100 GEN. Overview of Nucleic Acids & Uric Acid. 3. DURATION OF COURSE . BACTERIOLOGY CLIN. Radiation hazard. BACTERIOLOGY SYST.PART-2 (FIRST DMLT) TOTAL MARK. PRACTICAL ORAL 25 15 . Water & Fat soluble Vitamin. Flourine. 6. MICROBIOLOGY MYCOLOGY PRACTICAL 30 ORAL 20 BIOCHEMISTRY THEORY 60 1. Buffers.

Grouping and typing of donor's blood.-Typing.. . Calculation . Organization. Write Cell Count. microscopic. Preparation of Scminal Fluid for analysis. Total protein (Esbachs) etc. Colorimetric method . Normal values.C. Routine tests viz. Bile salt. Factors affecting ESR.Sahali’s method.C count .Interpretation.CSF . Introduction to haematology. Differential white cell count. Counting methods . ABO blood group system and incompatibility. Normal value and interpretation. Preservation and Storage .B. Macro and Micro method ..details PAPER-I PATHOLOGY Total Marks: 150 Theory -100. . Complication of blood transfusion. Bile pigment. Blood Transfusion Procedure. Recording the details and storage of blood. ESR.. Westergren’s... . chemical. Others.. Practical-30. . Ascitic fluid. Specific gravity. Count. Storage of blood. Cell separator and transfusion of various components of blood like Plasma and Platelet Separation . Collection of blood. Rh. Introduction. Romanosky Stains . -Major Cross Matching -Minor Cross Matching. Commb’s test. Operation and Administration of Blood Bank and anticoagulants. Wintrobe’s. Recording. Selection and Screening of donors. Rh blood group system and incompatibility . Identification. Packed Cell Volume (Haematocrit). Indirect . Technique of grouping and cross matching . Venipuncture.Blood.B. Indirect . Faecal analysis for occult blood examination. RBC diluting Fluid . Maintenance. Other tests viz. ORAL AND PRACTICAL ABO-Blood Grouping : Slide technique.. Absolute Eosinophil Count Direct/Indirect smear examination. Procedure for W. Pleural fluid . Oral-20 IMMUNO HAEMATOLOGY & BLOOD BANKING THEORY. Physical. Blood Collection. Collection. Calculation . Sugar.PART-2 (FIRST DMLT). Importance and Limitation . WBC diluting fluid . ... Chyle.. Coomb’s Test –Direct. Human blood group antigens. Direct. Urobilin Ketone bodies. Cyanmethaemoglobin method. Albumin and Phosphates. Preparation of aspiration fluids. Red Cell Counts. CLINICAL PATHOLOGY & HAEMATOLOGY Urine analysis. cleaning of various equipments used in the blood bank. Haemocytometer and procedure for R. Morphology of write cell. Drawing of blood – Asepsis. Collection of blood sample and anticoagulants.. . Haemoglobin estimation . Donor Screening and Selection. . Measurance. Cross matching.

Total RBC Count..Coagulation test: . Absolute Eosnophil count. POV (Haematocrit) 21. Reticulocyte 14. Demonstration of colorimetric Hb estimation. Analysis of aspiration fluids. Osmotic fragility test.. 5. Electrophoresis Test 25. Direct. Osmotic Fragility Test 23. Examination of Blood Parasites.Estimation of G-6-PD. Method-Interpretation . ESR-Westergren’s&Wintrobes 20. Use of Microscope. Clot retraction test . Calculation and importance of Reticulocyte count. 12. Total platelet count. Comments’ on peripheral smear 26. Prothrombin time . Staining and examination of different smears. Faeces occult blood test.Interpretation . Principle of Electrophoresis. Seminal fluid analysis. Microfilaria 18.. 2. Malaria Parasite. 17. 13. Analysis of Urine for routine and others tests. Differential count of Leucocyte. . Estimation of G-6-PD 24. Comments on peripheral smear. Indirect 16. Direct . 8. LE Cell phenomenon. Indirect 15. 11. Haemoglobin estimation –Sahali’s 9.Interpretation of result . Platelet count. Whole blood coagulation time .Sickle Cell Preparation . LE Cell Phenomenon. care and Maintenance. Bleeding time . Total Leucoyte Count. Urine microscopic examination.. Sickle Cell Test 22. 4. 7. Red Cell Indices. Preparation of bone marrow aspiration and trephine biopsy. Bleeding time and clotting time. . 3. 10. ORAL AND PRACTICAL 1. Prothromibin time-Demonstration 19. 6.

Leprae).Staphylococci. Pyrexia of unknown origin (P. pulmonary Tuberculosis). Deep Freeze. Vacuum pump. VDRI rotation Centrifuge machine. Candia Aspergillums. media pouring chamber EUSA reader. subculture and maintenance of stock culture. Water bath. glassware’s etc.O) . Proteus. Salmonella. Spirochetes CLINICAL MICROBIOLOGY . Mechanical methods.Normal microbial flora of human body. Microbes and their classification . Food Poisoning . Bacillus. Vibrio . Inspissator.Coll. BOD incubator. Bacilli – Corynebacterium. Bacterial Filters. Basic idea on Actinocycetes.Brief idea on Dermatophytes. Study of different . Safe disposal of contaminated media etc. Morphology of bacteria. Nonsporing anaerobes.BacterimiaPyaemia. LCB mount. Septicemia.Pseudomonas. etc. Opportunistic Infection MYCOLOGY . Mycobacterium (M.U. Refrigerator. Enterobacteriaceae. M.Klebsiella. E.. Cryptococcus and Opportunistic Fungi. Collection and transport of specimen .  Hot air oven.Meningitis.etc Anaerobic culture. Practical-30. Morphology of Fungi. syringe. microscopes. Common Laboratory equipments and uses  Different microscope. Inoculation techniques. Preparation and uses of culture media.Pneumococci. Culture media and methods. Meniogococci. Laboratory diagnosis of Fungi (KOH prepn. Autoclave. Shiegella. Sterilization of media. Culture methods and identification of bacteria Sterilization and Disinfection  Physical Chemical. Oral-20 GENERAL BACTERIOLOGY  History of Microbiology.Classification of pathogenic Fungi. Motional requirements of bacteria. incubator. Cocci . Clostridium. Respiratory Infection (Sore throat pneumonic. tuberculosis. Antimicrobial susceptibility tests SYSTEMIC BACTERIOLOGY More importance should be given to culture methods and identification of bacteria that other properties like Pathogenesis etc.) . Nosocomial Infections.PAPER-II MICROBIOLOGY Total Marks: 150 Theory -100.Isolation and identification of bacteria (Cultural characters biochemical reaction) serotyping etc. . Gonococci. streptococci.Ricketsiaeae.

percent solution. -Isolation of bacteria in pure culture and Antibiotic sensitivity. . Maintenance and preservation of stock culture. Study of fungi by wet preparation.Investigation of various common epidemics . culture etc.any measures. Stool. slide agglutination and other tests. stain.U.Handling and care of microscopes.e..O. slides petridishes pipettes. Neisser’s stain. Lactophenol cotton blue staining for fungi. Gram’s stain. Z. Vaginal fluid examination. Rashes. KoH preparation for fungi... Food poisoning. First Aid in Laboratory accidents and general precaution. I.e. Wet preparations like Hanging drop preparation. i. molar solution. Labeling. Anaerobic jor and other methods of anaerobic culture. -Identification of common bacteria by studying their morphology. staining. Other special stain.I. cultural character. Keeping records after final computerization. Geimsa stain... Aspiration fluid etc). syingesetc). Study of nosocomial infection.Simple stain.. Bone marrow. CSF urine swabs faeces. Conversant with S.N. Meningitis . For microbiological diagnosis. Gastroenteritis.PRACTICAL & ORAL MARKS . Receipts. . Sputum. Collection & transport of clinical specimens (Blood CSF Urine. CLINICAL MICROBIOLOGY:Approach to various clinical syndromes Collection transport and processing of various clinical specimens . beakers. funnels. Swabs. part-dilution. Various staining technique:.. etc.30+20 General Introduction-Safety measures in the laboratory. strength of a solution. Leishman stain. Spore stain. Operation and maintenance of laboratory equipments. Biochemical reactions. Encephalitis. . Albert’s stain. Conversant with Fundamental Chemistry. blood.. Cholera. recording and dispatching clinical specimens. . unit system for reporting. use of indicators. Care and cleaning of all glassware (test tubes. normal solutions etc. Negative stain. P.

Iodine. 4. Physiological Urine. Molarity. 2. factors regulating. Overview of Nucleic Acids & Uric Acid. Flourine. institution 2 clinical application) 4. Calcium. Tests for Carbohydrate. Overview of Iron. Radioisotopes. indicators. Radiation hazard. Calcium. 3. Plasma protein. 3. etc 6. 5. Tests for Iron. Glass ware cleaning. Practical-25. 5. record maintenance. Chemistry of a) Carbohydrates including peptidoglycan b) Fat c) Proteins& Amino acid 2. Water & Fat soluble Vitamin. Enzymes (Classification. Tests forProteins &Amino Acids. 6. Laboratory safety. Pipettes. . RSA. Oral-15 Theory 1.PAPER-III BIOCHEMISTRY Total Marks: 100 Theory -60. Flourine. Iodine. Buffers. Practical 1.

7.400. 4. 6. Glucose Homeostasis. overview DM. THE FINAL EXMINATION WILL BE CONDUCTED TEN MONTHS AFTER SECOND SEMESTER. TOTAL MARKS FOR WHICH WILL BE 400. . Alimentary function test. HGAIC. Lipoprotein & Hyper Lipoprotein. 5. 2. Liver function test.TOTAL MARKS. Renal function test. PRACTICAL ORAL 25 15 NB. 3. Thyroid function test. SECOND SEMESTER EXAMINATION WILL BE CONDUCTED TEN MONTHS AFTERFIRST SEMESTER EXMINATION.PART-3 (FINAL DMLT) PAPER PAPER-I PAPER-II SUBJECT PATHOLOGY THEORY HISTOTECHNOLOGY CYTOLOGY MUSEUM STUDY ORAL PRACTICAL MICROBIOLOGY THEORY IMMUNOLOGY SEROLOGY PARASITOLOGY VIROLOGY ANIMAL CARE PRACTICAL 30 ORAL PAPER-III BIOCHEMISTRY THEORY MARKS TOTAL MARKS DURATION 100 150 10 MONTHS 150 10MONTHS 100 10 MONTHS 30 20 100 20 60 1. Water & Electrolytic Balance.

Paraffin blockmaking . Special staining .Processing of the tissue. (a) Staining :. Preparation of adhesives to fix the section to the slide. Collection of specimen. . embedding and section and . Stropping. PAS.. Mounting . Mounting of sections. . 1. Neutralization and processing. Dyes and their properties . Vencos for calcium . Frozen section. Fixation of tissue: Classification of fixatives. Reticulin . Fixation . Preparation and fixation of cytology smears and .. Nuscum Techniques. Types of specimens and preservation. Presentation AUTOPSY TECHNIQUE:Assisting in autopsy. Tissue and their function. Staining technique with haematoxylin and eosin.Methods of examination of tissues and cells. . Clearing . . Sharpending of the knife.. . Preparation and fixation of smears. Section Cutting: ... Meason Trichrome . Common special stains – . Tissue processing : .Papanicolaoue’s staining techniques.Fixation. Impregnation . . Mounting of actions . . Decalcification . Waste disposal and safety in laboratory. Preservation . Simple Fixatives and their properties. Theory of staining . Embedding. Preservation of organs and . Lipid.PART-3 (FINAL DMLT) -details FINAL PAPER –I PATHOLOGY Total Marks: 150 Theory -100. MOG staining /HE staining. Reception of specimen. Decalcification : . . Dehydration . . . Sex chromatin staining. Labeling and fixation . Men – Geison . . MUSEUM STUDY Introduction. Microtomes and microtome knives – sharpening of knife. Routine H & E. (a) Exfoliative Cytology and Fine needle aspiration cytology : . Preparation of fixation . Detection of end point. Oral-20 HISTOTECHNOLOGY.. Techniques of section cutting . processing. . Mucicamine . Preparation of fixatives and . Practical-30. Papanicolaous staining technique/MCC staining/HE staining/. ORAL AND PRACTICAL MARKS-30+20 Histotechnology and Cytology . Microtome use – Honing. . CYTOLOGY. Fe. Cell. reparation of slides. decalcifying fluid.

Macrophage bacterial and fungal study b) Concentration techniques for examination of faeces.classification & different skin tests used for diagnosis. Intestinal & Tissue protozoa (E. Vaccines-classification & uses.FINAL PAPER-II MICROBIOLOGY Total Marks: 150 Theory -100. RBC. feeding and Breeding of laboratory animals. Giardia Primary Amoebic meningo-encephalitis) . Intestinal nematodes.General Characters of viruses. Embryonated egg inoculation. Antibody (Immunoglobulin’s). Immuno-prophylaxis & Immunization schedule. Intestine.. Puscells. Blood smear examination for malaria parasite L.(all theory discussion).histolylica.) ANIMAL CARE . micro filarial etc. Tissue culture techniques . Flukes of liver and . HIV. Hepatitis viruses.Immunity –Basic principles and classification. PARASITOLOGY . Complement system. Oral-20 IMMUNOLOGY AND SEROLOGY Emphasis on principal and uses/application . Autoimmunity – Basic concept. Tapeworms.Handling. Immunodeficiency diseases including AIDS –in brief... Collection and transport of virological specimens .Oncogenic viruses in brief. Antigen. Antigen – Antibody reactions. Rabies. Measles.Care of sheep and procedure to draw blood from sheep. Rata.Malaria parasite. Filarial worms and other tissue nematodes VIROLOGY . Hypersensitivity. Dengue) .D. Virology . Leishmanial parasites. (Polio.Introduction & classification of medically important parasites. a) Saline and lodine preparation of faeces for identification of Ova Cysts. preservation of faecalmatereials for examination of parasites. Practical-30. Practical & Oral Marks-30+20 (Serology + Parasitology + Virology + Animal Care) Parasitology Collection. Classification in brief and name of the diseases they produce.Laboratory diagnosis of viral infections (various methods of virus culture. serology etc. transportation. bodies.

Lepromin test. Serology -Widal test and preparation of Salmonella antigens. Get diffusion techniques and .. Casoni’s test. VDRI Test.Serological tests for diagnosis of common viral diseases.Immuno electro phoresis. Toxoplasmosis) ELISA test RIA Test. ASO. Other tests. CRP. Detection of Antigen / Antibody for Malarial (ICT). Pregnancy Test. . HIV surveillance lab and EUSA / Rapid tests. Australia Antigen. Assay of immunoglobulins Diagnostic skin tests - Tuberculin test (montoux test). Optimal Test. Latest agglutination tests for (RA.

2. Practical-25.. Biochemical tests of CSF.Glucose Tolerance Tests . SECTION-B CLINICAL ENZYMOLOGY & ORGANIZATION Fundamentals of analytical bio-chemistry and instrumentation. Pancreatic Function Tests-2 Serum Amylase. preservation Physical characteristics. Clinical enzymology – Diagnostic enzymes.12 3.FINAL PAPER-III BIOCHEMISTRY Total Marks: 100 Theory -60. 4.3 24 hr collection.Auto analyzers .Chromatography . Serum Lipase.Electrophoresis ORAL AND PRACTICAL List of Practical’s in Clinical Bio-chemistry Determination in Blood/Serum of . Iso-zymes. clearance tests.Colorimeter and spectrophotometer .Flame photometer .3 5.. Liver function tests. Oral-15 CLINICAL BIOCHEMISTRY SECTION-A ORGAN FUNCTION TESTS 1. Fundamentals of Analytical Bio-chemistry & Instrumentation . 1. Renal FunctionTests.Urea . Endocrine Function Testes – Thyroid Function Tests.2 2.Centrifuges . Gastric Function Tests-1 6. Serum Trypsin.Analytical balance .

- Creatinine Uric Acid Cholesterol. TSH . Lipid Profile Total serum protein and albumin T3. HDL Cholesterol. T4. Triglycerides.