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PHYTOPLANKTON 96-h GROWTH TEST- CELLS NUMBER

Test species :
Dilution water :
Test Date :
Project Name :
Nominal
Conc.
(mg/L)

Isochrysis sp
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(
)

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C

Kontrol

0.56

1.0

1.8

3.2

5.6

I% =

C-T

x 100

C
S% =

T-C
C

x 100

Count
1
( x 104)
15
18
19
24
31
28
23
30
27
19
21
19
30
31
28
49
35
37

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
12
17
21
25
27
31
27
25
28
23
23
23
27
32
26
47
37
39

Mean
cells/mL
( x 104)
13.50
17.50
20.00
24.50
29.00
29.50
25.00
27.50
27.50
21.00
22.00
21.00
28.50
31.50
27.00
48.00
36.00
38.00

Mean
cells/mL
( x 104)
17.00

27.67

26.67

21.33

29.00

40.67

Recorder : ________
Notes
: __________________________________
__________________________________
__________________________________

- CELLS NUMBER

PHYTOPLANKTON 96-h G

100mL
1x10.000
Cadmium
24h
%
Inhibition

_______________________
_______________________
_______________________

Test species :
Dilution water :
Test Date :
Project Name :
%
Stimulation

Nominal
Conc.
(mg/L)

Isochrysis sp
FNSW
5/6/2014
Kompetitip 2014 Nano

Actual
Conc.
(
)

Rep.
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C
A
B
C

Kontrol

0.56

1.0

1.8

3.2

5.6

I% =

C-T

x 100

C
S% =

T-C
C

x 100

OPLANKTON 96-h GROWTH TEST- CELLS NUMBER

ochrysis sp
NSW
5/6/2014
ompetitip 2014 Nano
Count
1
( x 104)
17
26
20
18
17
19
12
15
13
14
10
13
13
10
11
12
9
7

Test Volume :
Initial Cell Density :
Test Material :
Project ID. :
Count
2
( x 104)
20
23
23
19
15
17
17
17
15
16
12
14
11
14
13
16
9
10

Mean
cells/mL
( x 104)
18.50
24.50
21.50
18.50
16.00
18.00
14.50
16.00
14.00
15.00
11.00
13.50
12.00
12.00
12.00
14.00
9.00
8.50

Mean
cells/mL
( x 104)

100mL
1x10.000
Cadmium
48h
%
Inhibition

21.50

17.50

18.60%

14.83

31.01%

13.17

38.76%

12.00

44.19%

10.50

51.16%

Recorder : ________
Notes
: __________________________________
__________________________________
__________________________________

%
Stimulation

6 I% = C-T x 100 C S% = T-C x 100 Count 1 ( x 104) 29 18 19 14 18 29 20 14 24 14 10 14 10 14 13 16 17 10 Test Volume : Initial Cell Density : Test Material : Project ID.00 14.50 15.50 20.50 14.50 28. A B C A B C A B C A B C A B C A B C Kontrol 0.33 13.67 Recorder : ________ Notes : __________________________________ __________________________________ __________________________________ C j .00 10.2 5.17 13.00 Mean cells/mL ( x 104) 22.50 13.50 16.33 13.50 9.56 1.PHYTOPLANKTON 96-h GROWTH TEST.17 19.CELLS NUMBER Test species : Dilution water : Test Date : Project Name : Nominal Conc. ( ) Rep. : Count 2 ( x 104) 27 21 21 14 19 27 19 15 24 20 9 12 10 17 16 17 14 8 Mean cells/mL ( x 104) 28.00 18.8 3.00 15.00 17.0 1.00 9.00 19.50 20.00 19.50 24.50 14. (mg/L) Isochrysis sp FNSW 5/6/2014 Kompetitip 2014 Nano Actual Conc.

56 14.37% 0.74% 3.2 39.CELLS NUMBER PHYTOPLANKTON 96-h GR 100mL 1x10.6 ______________________ ______________________ ______________________ I% = C-T x 100 C S% = T-C C x 100 .0 41. ( ) Rep.8 40. A B C A B C A B C A B C A B C A B C Kontrol 10. (mg/L) Isochrysis sp FNSW 5/6/2014 Kompetitip 2014 Nano Actual Conc.000 Cadmium 72h % Inhibition Test species : Dilution water : Test Date : Project Name : % Stimulation Nominal Conc.07% 1.26% 5.48% 1.

50 28.00 33.50 26.00 33.50 Mean cells/mL ( x 104) 100mL 1x10.50 20.50 19.33 20.00 36.50 22.00% 17.50 12.00 15.50 23.50 29.09% 21.45% 20.83 40.09% 29.67 51.50 24.50 20.50 44.50 17.17 34. : Count 2 ( x 104) 39 34 35 24 20 28 22 24 20 25 12 27 27 28 35 22 15 15 Mean cells/mL ( x 104) 41.000 Cadmium 96h % Inhibition 36.67 24.00 22.50 24.CELLS NUMBER ochrysis sp NSW 5/6/2014 ompetitip 2014 Nano Count 1 ( x 104) 43 32 37 21 21 31 25 21 19 24 13 22 26 28 32 18 16 20 Test Volume : Initial Cell Density : Test Material : Project ID.TOPLANKTON 96-h GROWTH TEST.82% Recorder : ________ Notes : __________________________________ __________________________________ __________________________________ % Stimulation .

00 19.50 28.00 21 32.PHYTOPLANKTON 96-h GROWTH TEST.00 25 56.00 17.00 21.50 Recorder : ________ Notes : __________________________________ __________________________________ __________________________________ .00 10 18. (µ/L) Kontrol 2 10.50 24.50 21.83 17.00 38 100.50 36.67 29.50 21.50 30.50 21.00 29. : Count 2 ( x 104) 27 23 22 29 29 33 35 27 25 21 23 23 29 23 23 18 32 24 20 13 18 Mean cells/mL ( x 104) 27.00 23.00 Mean cells/mL ( x 104) 23.50 23.CELLS NUMBER Test species : Dilution water : Test Date : Project Name : Nominal Conc. (µ/L) Isochrysis sp FNSW 5/6/2014 Kompetitip 2014 Nano Actual Conc.50 27. A B C A B C A B C A B C A B C A B C A B C x 100 C S% = T-C C x 100 Count 1 ( x 104) 28 21 21 26 28 32 38 20 22 22 21 25 30 20 19 17 28 24 19 15 20 Test Volume : Initial Cell Density : Test Material : Project ID.50 14.00 32 100 *) 62 I% = C-T Rep.83 22.00 24.50 23.50 32.00 24.00 19.50 22.50 22.50 27.

00 10 18.00 21 32.000 Copper 24h % Inhibition _______________________ _______________________ _______________________ Test species : Dilution water : Test Date : Project Name : % Stimulation Nominal Conc.00 32 100 *) 62 I% = C-T Rep. (µ/L) Isochrysis sp FNSW 5/6/2014 Kompetitip 2014 Nano Actual Conc.00 38 100.. A B C A B C A B C A B C A B C A B C A B C x 100 C S% = T-C C x 100 .00 25 56.CELLS NUMBER PHYTOPLANKTON 96-h 100mL 1x10. (µ/L) Kontrol 2 10.

67 44.76% Recorder : ________ Notes : __________________________________ __________________________________ __________________________________ .00 11.50 11.00 12.67 16.00 10.57% 9.00 9.00 11.50 17.50 18.00 13.00 20.00 19.33% 14.TOPLANKTON 96-h GROWTH TEST. : Count 2 ( x 104) 17 18 19 13 21 21 10 14 8 13 15 15 18 14 13 10 12 7 10 12 10 Mean cells/mL ( x 104) 18.50 14.50 20.000 Copper 48h % Inhibition % Stimulation 17.10% 15.17 13.00 9.00 8.CELLS NUMBER ochrysis sp NSW 5/6/2014 ompetitip 2014 Nano Count 1 ( x 104) 20 10 21 14 20 19 12 10 11 12 19 17 20 13 10 8 8 9 8 10 8 Test Volume : Initial Cell Density : Test Material : Project ID.00 48.19% 9.00 Mean cells/mL ( x 104) 100mL 1x10.50 9.00 2.00 16.86% 10.50 20.83 38.00 13.00 9.50 12.

00 21.50 55.00 44.50 18.50 23. : Count 2 ( x 104) 27 35 54 41 48 34 30 23 21 32 40 28 28 18 29 17 23 31 22 17 13 Mean cells/mL ( x 104) 25.83 40.00 25 56.PHYTOPLANKTON 96-h GROWTH TEST.00 21 32.67 Recorder : ________ Notes : __________________________________ __________________________________ __________________________________ .00 26.33 25.50 33.00 25. A B C A B C A B C A B C A B C A B C A B C x 100 C S% = T-C C x 100 Count 1 ( x 104) 23 44 56 47 45 27 31 19 29 33 42 20 23 18 23 19 20 36 21 19 14 Test Volume : Initial Cell Density : Test Material : Project ID.00 25. (µ/L) Kontrol 2 10.00 10 18.CELLS NUMBER Test species : Dilution water : Test Date : Project Name : Nominal Conc.50 21. (µ/L) Isochrysis sp FNSW 5/6/2014 Kompetitip 2014 Nano Actual Conc.17 24.50 41.00 24.00 32 100 *) 62 I% = C-T Rep.00 13.33 17.50 21.00 38 100.00 46.50 30.50 32.00 18.00 32.50 30.00 39.50 Mean cells/mL ( x 104) 39.50 18.

A B C A B C A B C A B C A B C A B C A B C x 100 C S% = T-C C x 100 .91% 100.00 38 38.84% 56.00 10 35.000 Copper 72h % Inhibition Test species : Dilution water : Test Date : Project Name : % Stimulation Nominal Conc.26% ______________________ ______________________ ______________________ I% = C-T Rep.00 21 18.98% 18.00 25 41.CELLS NUMBER PHYTOPLANKTON 96-h GRO 100mL 1x10.00 32 55. (µ/L) Kontrol 2 10.65% 100 *) 62 1. (µ/L) Isochrysis sp FNSW 5/6/2014 Kompetitip 2014 Nano Actual Conc.41% 32.

56% 31.50 29.00 49.33 64.00 13.CELLS NUMBER ochrysis sp NSW 5/6/2014 ompetitip 2014 Nano Count 1 ( x 104) 40 44 41 53 45 50 41 46 34 37 32 26 31 41 28 18 12 15 14 9 11 Test Volume : Initial Cell Density : Test Material : Project ID.20% 11.50 9.PLANKTON 96-h GROWTH TEST.50 45.85% 31.50 37.50 39. : Count 2 ( x 104) 42 43 47 45 49 49 34 45 35 38 30 25 28 32 30 21 11 15 13 10 10 Mean cells/mL ( x 104) 41.83 48.00 49.17 73.93% Recorder : ________ Notes : IC50: 48.50 Mean cells/mL ( x 104) 100mL 1x10.50 29.50 37.00 43.07% 15.50 44.00 19.50 34.50 36.000 Copper 96h % % Inhibition Stimulation 42.50 31.00 25.00 47.67 26.50 11.50 10.33 26.50 15.17 8.2 ppb __________________________________ __________________________________ .

00 24.00 31.00 21.00 42.50 36.00 32.00 18.PHYTOPLANKTON 96-h GROWTH TEST.8 3.0 1.00 17. ( ) Rep.00 25.2 5.50 27.33 Recorder : ________ Notes : __________________________________ __________________________________ __________________________________ .00 25.83 42.6 I% = C-T x 100 C S% = T-C C x 100 Count 1 ( x 104) 19 16 18 55 28 25 30 20 25 36 18 46 25 23 33 26 39 52 Test Volume : Initial Cell Density : Test Material : Project ID. (mg/L) Chaetoceros gracilis FNSW 5/6/2014 Kompetitip 2014 Nano Actual Conc.50 16.83 26.50 55.50 53.50 29.56 1.CELLS NUMBER Test species : Dilution water : Test Date : Project Name : Nominal Conc.17 33.50 Mean cells/mL ( x 104) 17. : Count 2 ( x 104) 18 16 19 52 29 29 34 22 26 36 17 50 25 25 30 32 46 59 Mean cells/mL ( x 104) 18.50 48.67 36.50 28.33 26. A B C A B C A B C A B C A B C A B C Kontrol 0.

2 5.6 I% = C-T x 100 C S% = T-C C x 100 .0 1.56 1.8 3.000 Cadmium 24h % Inhibition _______________________ _______________________ _______________________ Test species : Dilution water : Test Date : Project Name : % Stimulation Nominal Conc. A B C A B C A B C A B C A B C A B C Kontrol 0.CELLS NUMBER PHYTOPLANKTON 96-h 100mL 1x10. (mg/L) Chaetoceros gracilis FNSW 5/6/2014 Kompetitip 2014 Nano Actual Conc. ( ) Rep..

00 15.00 14.CELLS NUMBER haetoceros gracilis NSW 5/6/2014 ompetitip 2014 Nano Count 1 ( x 104) 10 7 14 18 10 13 10 14 13 18 19 19 16 10 19 12 14 11 Test Volume : Initial Cell Density : Test Material : Project ID.00 17.67 15.50 7.00 11.83 12.50 18.00 20.00 18.00 20.00 18.50 10.67 18.50 10.00 Mean cells/mL ( x 104) 100mL 1x10.00 12. : Count 2 ( x 104) 11 7 15 19 10 12 10 14 15 18 17 21 18 10 22 12 16 11 Mean cells/mL ( x 104) 10.50 12.TOPLANKTON 96-h GROWTH TEST.67 12.00 10.67 Recorder : ________ Notes : __________________________________ __________________________________ __________________________________ % Stimulation .000 Cadmium 48h % Inhibition 10.00 14.00 14.67 13.

50 26.8 3.00 21.00 27.2 5.67 21.50 22. A B C A B C A B C A B C A B C A B C Kontrol 0. (mg/L) Chaetoceros gracilis FNSW 5/6/2014 Kompetitip 2014 Nano Actual Conc.00 31.67 Recorder : ________ Notes : __________________________________ __________________________________ __________________________________ .50 28. ( ) Rep.50 34.50 23.00 24.6 I% = C-T x 100 C S% = T-C C x 100 Count 1 ( x 104) 21 25 33 29 27 22 32 23 19 25 27 16 20 19 16 20 26 21 Test Volume : Initial Cell Density : Test Material : Project ID.56 1.PHYTOPLANKTON 96-h GROWTH TEST.50 19.50 25.00 20.50 20.00 27.50 Mean cells/mL ( x 104) 27.50 18.50 26.50 23. : Count 2 ( x 104) 27 29 30 27 27 25 37 27 21 28 26 20 23 26 23 20 23 26 Mean cells/mL ( x 104) 24.17 26.CELLS NUMBER Test species : Dilution water : Test Date : Project Name : Nominal Conc.00 26.0 1.00 23.17 22.

2 5. A B C A B C A B C A B C A B C A B C Kontrol 0.56 1.6 I% = C-T x 100 C S% = T-C C x 100 . ( ) Rep.0 1.8 3.000 Cadmium 72h % Inhibition ______________________ ______________________ ______________________ Test species : Dilution water : Test Date : Project Name : % Stimulation Nominal Conc. (mg/L) Chaetoceros gracilis FNSW 5/6/2014 Kompetitip 2014 Nano Actual Conc.CELLS NUMBER PHYTOPLANKTON 96-h GR 100mL 1x10.

50 27.00 Mean cells/mL ( x 104) 100mL 1x10.00 20.00 33.50 27.00 45.17 31.00 24.TOPLANKTON 96-h GROWTH TEST.00 46.00 51.40% 25.00 23.00 22.00 24.00 21.CELLS NUMBER haetoceros gracilis NSW 5/6/2014 ompetitip 2014 Nano Count 1 ( x 104) 69 47 51 26 31 34 45 26 28 22 22 24 28 20 26 20 28 21 Test Volume : Initial Cell Density : Test Material : Project ID.50 30.20% 34.10% Recorder : ________ Notes : __________________________________ __________________________________ __________________________________ % Stimulation .67 57.33 43.17% 23.50 29.83 53.000 Cadmium 96h % Inhibition 55.00 28.16% 23.00 34.50 27.67 37.50 57. : Count 2 ( x 104) 69 43 52 28 35 34 48 29 32 22 26 25 30 23 28 20 28 25 Mean cells/mL ( x 104) 69.

00 30.CELLS NUMBER Test species : Dilution water : Test Date : Project Name : Nominal Conc.33 28.50 26.00 21.50 26.50 28.67 17.50 18.00 10 18. : x 100 C Kontrol 10 18 32 56 100 100* 24h 45.83 34.50 30.50 21.00 32 100 *) 62 I% = C-T Rep.50 32.83 20.PHYTOPLANKTON 96-h GROWTH TEST.00 21 32.50 14.00 25 56.00 38 100.50 33.33 48h 72h 96h .50 18.67 17.00 Mean cells/mL ( x 104) 45.00 11.83 34.00 43.83 20.00 18. (µ/L) Chaetoceros gracilis FNSW 5/6/2014 Kompetitip 2014 Nano Actual Conc.00 45.00 22.00 31.00 28.33 28.50 13.00 20.00 38. A B C A B C A B C A B C A B C A B C A B C Count 1 ( x 104) 59 42 27 29 30 41 35 25 36 29 14 11 16 18 20 12 19 21 25 31 27 T-C Count 2 ( x 104) 65 49 33 31 30 46 32 27 41 28 14 11 20 24 25 15 23 20 27 31 29 Mean cells/mL ( x 104) 62.50 32. (µ/L) Kontrol 2 10.33 Recorder : ________ Notes : __________________________________ __________________________________ __________________________________ x 100 C S% = Test Volume : Initial Cell Density : Test Material : Project ID.00 30.

00 Kontrol 10 18 32 56 100 .00 30.00 5.00 15.00 25.50.00 45.00 20.00 40.00 0.00 35.00 10.

00 10 28.73% 18. (µ/L) Kontrol 2 24.000 Copper 24h % Inhibition Test species : Dilution water : Test Date : Project Name : % Stimulation Nominal Conc..18% 100 *) 62 _______________________ _______________________ _______________________ I% = C-T Rep.00% 100. (µ/L) Chaetoceros gracilis FNSW 5/6/2014 Kompetitip 2014 Nano Actual Conc.00 25 55.00 21 61.27% 56.09% 32.73% 10.00 32 38. A B C A B C A B C A B C A B C A B C A B C x 100 C S% = T-C C x 100 .00 38 60.CELLS NUMBER PHYTOPLANKTON 96-h 100mL 1x10.

32 56 100 100* .

22% 19.YTOPLANKTON 96-h GROWTH TEST.00 22.00 11.00 16.50 21.00 19.55% 17.85% 18.17 23.50 33.50 21.CELLS NUMBER haetoceros gracilis NSW 5/6/2014 ompetitip 2014 Nano Count 1 ( x 104) 24 19 32 19 21 24 23 15 12 15 22 18 12 11 18 21 14 20 20 17 18 Test Volume : Initial Cell Density : Test Material : Project ID.50 16.00 13.50 21.83 31.00 20.50 17.000 Copper 48h % Inhibition 26.50 20.50 19.50 15.50 29.33 26.50 27.11% 18.50 22.30% 14.50 18.50 19.33 45.66% Recorder : ________ Notes : __________________________________ __________________________________ __________________________________ % Stimulation .00 15.67 9. : Count 2 ( x 104) 25 22 35 26 22 30 21 18 18 16 20 20 14 12 19 22 18 21 19 18 20 Mean cells/mL ( x 104) 24.67 28.00 Mean cells/mL ( x 104) 100mL 1x10.

.

33 27.00 25.00 25.CELLS NUMBER Test species : Dilution water : Test Date : Project Name : Nominal Conc. : Count 2 ( x 104) 36 56 38 35 30 33 30 34 28 22 29 25 31 28 24 28 23 30 27 31 30 Mean cells/mL ( x 104) 33.00 10 18.00 23.50 Recorder : ________ Notes : __________________________________ __________________________________ __________________________________ .00 28.00 32 100 *) 62 I% = C-T Rep.00 31.00 23.00 30.00 38 100. (µ/L) Chaetoceros gracilis FNSW 5/6/2014 Kompetitip 2014 Nano Actual Conc.00 28.50 64.67 32.50 31.PHYTOPLANKTON 96-h GROWTH TEST. A B C A B C A B C A B C A B C A B C A B C x 100 C S% = T-C C x 100 Count 1 ( x 104) 31 72 35 37 28 29 31 28 22 24 27 25 28 28 23 24 21 23 27 30 26 Test Volume : Initial Cell Density : Test Material : Project ID.00 29.00 30.00 22.00 26.00 36.00 Mean cells/mL ( x 104) 44. (µ/L) Kontrol 2 10.50 26.50 36.00 25 56.00 29.50 28.00 21 32.50 27.50 28.00 24.83 25.83 28.

.

00 21 43. (µ/L) Chaetoceros gracilis FNSW 5/6/2014 Kompetitip 2014 Nano Actual Conc.00 32 36. A B C A B C A B C A B C A B C A B C A B C x 100 C S% = T-C C x 100 .36% 10. (µ/L) Kontrol 2 28.45% 18.00 25 39.CELLS NUMBER PHYTOPLANKTON 96-h GR 100mL 1x10.19% 100 *) 62 ______________________ ______________________ ______________________ I% = C-T Rep.55% 56.00 38 44.28% 32.000 Copper 72h % Inhibition Test species : Dilution water : Test Date : Project Name : % Stimulation Nominal Conc.00 10 35.40% 100.

.

50 17.50 8.00 43.50 68.83 50.00% 25.00 16.06% 41.50 33.50 58.03% 47.50 45.67 47. : Count 2 ( x 104) 54 46 58 45 66 31 58 35 37 56 45 47 27 27 24 34 26 19 17 18 19 Mean cells/mL ( x 104) 51.50 52.00 28.50 15.00 27.17 8.83 19.50 58.CELLS NUMBER haetoceros gracilis NSW 5/6/2014 ompetitip 2014 Nano Count 1 ( x 104) 49 45 58 42 65 36 58 33 30 49 39 47 29 28 20 29 24 19 16 13 16 Test Volume : Initial Cell Density : Test Material : Project ID.71% 25.50 42.00 31.50 Mean cells/mL ( x 104) 100mL 1x10.00 33.50 25.00 34.50 65.50 22.00 47.000 Copper 96h % Inhibition 51.29% 16.TOPLANKTON 96-h GROWTH TEST.17 51.00 19.06% Recorder : ________ Notes : IC50: 38ppb __________________________________ __________________________________ % Stimulation .

.

000 580.000 175.39794 5.000 280.763428 5.000 275.672098 5.243038 .000 580.000 165.000 250.447158 5.000 470.525045 5.000 220.623249 5.531479 5.498311 5.278754 5.000 315.000 340.711807 5.000 155.658011 5.000 525.439333 5.000 335.217484 5.000 455.000 335.000 655.525045 5.763428 5.816241 5.720159 5.000 435.190332 5.000 420.000 5.515.000 190.342423 5.638489 5.