You are on page 1of 19

BIOFOSUN System

Operating Procedure Of
Susceptibility Test Panel
Shanghai Biofosun

Clinical Microbiology Laboratory


Testing Process
Sample
Collection
Bacterial
Culture

Grams
Staining
Grams
Staining

Subsidiary
Experiment

Bacteria
Purification

Test + Susceptibility
Data
Analysis

BIOFOSUN Operation System

1. Culture

Purificationfirst step of
accurate
testing

2.Identification Panel Selection

Basis of susceptibility test panel- grams staining, micrology


Purple (gram positive): panel GP48
Red (gram negative): panel GN48
Can also determine other basic forms such as coccus, bacillus
simultaneously.

3.Choosing Right Operation Procedure


basis of concentration selectionsubsidiary experiment
Gram negative: determine whether its Enterobacteriaceae or not by
magnesium oxide, KIA experiment
Results
Its Enterobacteriaceae if it is magnesium oxide negative, KIA test is
K/A or A/A, and choose concentration at 61%
Its not enterobacteriaceae if it is magnesium oxide positive, or magnesium
negative and KIA test is K/K. Then should choose concentration at 52%

Oxidase positive, but small colonies, requires specical


environment for fastidous bacteria, so chooses concentration
at 20%

3.Choosing Right Operation Procedure


Gram positive
1Gram positive: Catalase experiment
Staphylococcus aureus, Micrococcus and most coccus are
catalase positive
Streptococcus, Enterococcus are catalase negative
2Gram positive: smear stain of bacillus, Determine whether
the bacteria produce spores
Concentration selection
20% for gram positive coccus and gram positive none-bacilluse
28% for gram positive bacilluse

4. Microbial preparation

5. Filling and Incubation

Filling150ul/unit

30 or 35 incubation

6. Result Reading

Results at 4-6 hrsor 16-24 hrs

Identification Panel Operation


Procedure
GN/GP panel selection based on gram stain results
Choose the right method based on the result of subsidiary experiment

add 50L Tsolution in to 12mL IF cultures (except none-enterbacteriaceae )


Pick pure colony and make corresponding bacteria fluid at certain concentration
Pour the bacteria fluid in to sterile bacteria liquid slot
Add the liquid to identification panel by using pipettor
Incubation at 35 for 18-24 hrs (none-enterbacteriaceae at 30 )
Read the result

Panel Selection
Biofosuns panels in market
Enterbacteriaceae susceptibility panelME): for
Enterbacteriaceae
Vibrionaceae susceptibility panel MPfor vibrionaceae
Staphylococcus susceptibility panel(MSfor
Staphylococcus and enterococcus
Classification
Gram negative:
Oxidase negativeKIA is K/AA/AME panel
Oxidase positiveKIA is K/K MP panel
Gram positive:
Catalase positive
MS panel
Catalase negative, large colony
MS panel

Procedure
Sterile saline tube adjustment at 100
calibrate the concentration by using 0.5 McIntosh unit standard
Pick pure colony and make corresponding bacteria fluid at certain concentration
Add 60L of the solution in to 12mL IP cultures
Pour the bacteria fluid in to sterile bacteria liquid slot

Add 100 L the liquid to identification panel by using pipettor


Incubation at 35 for 18-24 hrs

Read the result

Operation Notice
Bacteria pure culturing is very important
bacteria purification or not, the quality of purification will
influence the accuracy of the identification and Susceptibility
Consequence without purification
1Cannot identify the real pathogenic bacteria
2High possibility of contamination
3Other material interference

Operation Notice

Accurate bacteria liquid concentration

Enterococci (35%T)

Enterococci(20%T)

Operation Notice

Choose appropriate incubation temperature

Staphylococcus xylosus30CStaphylococcus xylosus35C

Example Analysis
According to the oxidase test, ensure the
bacteria is enterbacteriaceae
Operate according to the enterbacteriaceae
VITEK system
Identification result is Enterobacter cloacae

Example Analysis
According to the oxidase test, ensure the bacteria is
enterbacteriaceae
Operate based on the procedure of BIOFOSUN system
No result

Choose gram negative, report as baumanii


Ensure the result as baumanii based on subsidiary experiment

Thank You