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Description

ProductSheet

Escherichiacoli(ATCC
25922)

Designation:FDAstrainSeattle1946[DSM1103,NCIB12210]
DepositedName:Escherichiacoli(Migula)CastellaniandChalmers
AntigenicProperties:SerotypeO6,Biotype1
ProductDescription:Doesnotproduceverotoxin.ThisorganismisaCLSIcontrolstrainforantimicrobial
susceptibilitytesting.Itisusedformediatesting,asanegativecontrolforLTtoxinproduction,andasaquality
controlstrainforAbbott,API,Autobac,BBL,bioMerieuxVitek,Biosynth,Difco,IDS,MicroMedia,MicroScan,
RocheDiagnostics,andSensititreproducts.Usedinsusceptibilitydisctestingofneomycin,colistin[colimycin],
kanamycin,cephalexin,gentamicins,cefamandole,cephalothin,tetracycline,cephaloglycin,cephaloridine
[cephalomycin],nalidixicacid,andchloramphenicol.

PleasereadthisFIRST

Propagation

StorageTemp.
Frozen:80Cor
colder
FreezeDried:2C
to8C
LiveCulture:See
Propagation
Section

BiosafetyLevel
1

IntendedUse
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Escherichiacoli(ATCC25922)

Medium
ATCCMedium18:TrypticaseSoyAgar/Broth
GrowthConditions
Temperature:37C
Atmosphere:Aerobic
PropagationProcedure
1. Openvialaccordingtoenclosedinstructions.
2. Usingasingletubeof#18broth(5to6mL),withdrawapproximately0.5to1.0mLwithaPasteuror
1.0mLpipette.Rehydratetheentirepellet.
3. Asepticallytransferthisaliquotbackintothebrothtube.Mixwell.
4. Useseveraldropsofthesuspensiontoinoculatea#18agarslantand/orplate.
5. Incubatethetubesandplateat37Cfor24hours.

Notes
ATCC25922isarecommendedreferencestrainforantibioticsusceptibilitytesting.Ithasbeenfoundthat
passageinbrothoftenresultsinachangeinMIClevels.Therefore,itisbesttokeepitonagarandtomake
stocksforstorageimmediately.Repeatedpassageisdiscouraged.

Colonieson#18agarareentire,glistening,circular,smooth,translucent,andlowconvex.
PurifiedgenomicDNAofthisstrainisavailableasATCC25922D5.

AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.

References
Referencesandotherinformationrelatingtothisproductareavailableonlineatwww.atcc.org.

BiosafetyLevel:1
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes
forHealth.

ATCCWarranty

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

Page1of2

TheviabilityofATCCproductsiswarrantedfor30daysfromthedateofshipment,andisvalidonlyifthe
productisstoredandculturedaccordingtotheinformationincludedonthisproductinformationsheet.ATCC
liststhemediaformulationthathasbeenfoundtobeeffectiveforthisstrain.Whileother,unspecifiedmedia
mayalsoproducesatisfactoryresults,achangeinmediaortheabsenceofanadditivefromtheATCC
recommendedmediamayaffectrecovery,growthand/orfunctionofthisstrain.Ifanalternativemedium
formulationisused,theATCCwarrantyforviabilityisnolongervalid.

Disclaimers
Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.

ProductSheet

Escherichiacoli(ATCC
25922)
PleasereadthisFIRST

StorageTemp.
Frozen:80Cor
colder
FreezeDried:2C
to8C
LiveCulture:See
Propagation
Section

BiosafetyLevel
1

IntendedUse
Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Escherichiacoli(ATCC25922)

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org
800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

Page2of2

WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproduct
sheet,ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliterature
andpatentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhas
beenconfirmedtobeaccurate.
Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.
ATCCisnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.While
reasonableeffortismadetoinsureauthenticityandreliabilityofstrainsondeposit,ATCCisnotliablefor
damagesarisingfromthemisidentificationormisrepresentationofcultures.
PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org
AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2013.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[12/09]