Itzel Rendon

Enzyme Lab Report

STEM 2
December 16, 2016
H2O2 Digests and makes O2!
Enzymes are proteins that can be found in our bodies. Enzymes
are helpful because they speed up chemical reactions. The enzymes
bind to substrates in order to either digest or synthesize. The
enzymes have to match the shape of of the substrate in order to work
efficiently. The shape of an enzyme can be affected by pH and
temperature. In this lab we measured the rate of reaction of
enzymes. The enzymes broke down H2O2 or hydrogen peroxide into
two different compounds. The hydrogen peroxide was broken down
into water and oxygen and in this lab we measured the oxygen level
caused by the rate of reaction. We thought that if we increased the
stirring rate then the rate of reaction would increase because of the
higher pressure that is caused.
Procedure
1. Check that you have all the equipment below.
Computer with Internet access and Vernier LoggerPro software
LabQuest Mini
Vernier Gas Pressure Sensor
Two-hole black rubber stopper (top)
Plastic tubing with Luer-lock connectors
20-200 L micropippetor set to 100 L
micropipette tips
50mL graduated cylinder
125 mL or 250 mL Erlenmeyer flask
Magnetic stirrer
Stirring bar
Thermometer
Metal clamp stand
At the central table:
Hydrogen peroxide
Catalase enzyme suspension
2. Check that every person is wearing apron and goggles.
3. Check that everyone who will touch the enzyme or liquids is
wearing thick latex gloves (blue and yellow gloves)
On the blank lines, write the initials of the teammate who completed
the task:
4.Open your laptop and open Logger Pro .
5. Click File Open and open the folder in Biology by
Vernier. Then choose 06 Enzyme (Pressure).

6.Connect the LabQuest Mini to the computer using the USB cable,
and connect the Gas Pressure Sensor to CH 1 of the LabQuest Mini.
Set up the laboratory apparatus as seen in the picture:
7. Measure out 50mL of 1.5% H2O2 and pour into an Erlenmeyer
flask.
8.Carefully place a magnetic stir bar in the flask.
9. Place the flask on a magnetic stir plate. Use a clamp to fasten the
flask to the ring stand as shown. Position the flask at the center of the
magnetic stirrer.
10. Test the stirrer at 100 rpm.
11.Stop the stirrer.
12. Use the plastic tubing with two Luer-lock connectors to connect
the two-hole rubber stopper assembly to the Gas Pressure Sensor as
shown in the image. The valve connected to the stopper should stay
closed during this investigation. Its closed when its flat, parallel to
the floor
Complete all the steps below quickly to complete your test reaction.
13. Start data collection: click green Collect button on Logger Pro.
14.Using a micropipette, add 100 L of enzyme suspension to the
contents of the flask.
15.IMMEDIATELY tightly seal the flask by placing the stopper in
and
HOLDING it in carefully.
16. IMMEDIATELY Turn the stir plate on to 150.
NOTE: If the pressure exceeds 130 kPa, the pressure inside the flask
will be too great and the rubber stopper will likely pop off. HOLD
DOWN the stopper or be ready to have uncovered chemicals on your
desk
WAIT 200 seconds (3.3 minutes) while data is collecting- Do NOT
click STOP. Data collection will automatically stop after 200
seconds.
17. Turn off the stir plate.
18. Carefully remove the stopper from the flask to relieve the
pressure.
19. Use a thermometer to test and record the temperature of the
liquid in your lab packet
20.Pour all chemical waste into a RED bucket.
21. Remove the magnetic bar.
22. Dispose of your used micropipette tip.
23. Take off your safety gear and place it neatly away.
24. Observe the graph generated using LoggerPro software.
25. Hold down Control on the keyboard and press j to zoom in the
graph.

26. Highlight the section of the graph where the slope is increasing,
by clicking and dragging your mouse across it.
27. Click the Analyze tab at the top of the page, and choose Linear
Fit. A statistics box will appear for your highlighted section of the
graph.
28. Record the slope of the line, m, as the rate of catalase activity in
kPa/s in your lab packet (page 13)
29. Click File, Save As, and save with the file name:
Example:
(Your Names) Sam, Anabel, Jenni, Jose Control Trial Logger Pro
Data
Email the graph to Ms. Lenowitz by following these final steps!
30. Click File and choose Print Graph. In the drop down menu of
printers, choose
Cute PDF Writer in the drop down list. This will export your graph
as a PDF file. Be patient- it takes a few seconds to pop up.
31. Save the PDF with the this file name:
(Your Names) Sam, Anabel, Jenni, Jose Control Trial Data PDF
32. Open your MESA email and Compose a new email to
glenowitz@mesacharter.org
33. Attach the PDF to the email.
34. Press send!
After doing the procedure, the results show that my hypothesis
is correct because my hypothesis stated that an increase in stirring
rate would also increase enzyme reaction rates. In the control trial,
the slope or rate of reaction was 0.37 kPa/min. The rate of reaction
increased dramatically after the increase of stirring rate. It went
from 0.37 kPa/min to 30.90 kPa/min. Since the enzymes were
moving faster due to the increase of stirring rate, they were also
working faster because it was easier for them to find another H2O2
molecule. It was easier because of the increase in kinetic energy
causing faster movements.
Even though, our hypothesis was correct and the stirring rate
also increased the enzyme reaction, it soon died down because after
all the enzymes were digested, there was nothing left to digest. A
way to prolong the rate of reaction would be maybe to add more
H2O2 and less enzyme suspension.