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1.

Colour Test
Table 4: Result of Colour Test for Sample Water Before and After the Application
of Mud ball Enzyme Using Wavelength of
Test

λ 400 and

Before

TUC

λ500

After

λ 400

λ500

λ 400

λ500

29.7

20.1

23.0

15.2

Table 5: Water quality standard from National Water Quality Standards For
Malaysia.
PARAMETE UNIT
CLASS
I
IIA
IIB
III
IV
V
R
Colour
TCU
15
150
150
Until today, there is no proper standards proposed by World Health Organization
(WHO) that had stated the guideline for the color requirement. It is subjective as
different people may have different tolerance to the color of the water. Besides,
color which is less than 15 TCU are usually acceptable to the user/consumer to
consume in comparison to the results which both of the water sample for before
and after application of mud ball enzyme are more than 15 TCU with range from
15.2 to 29.7 TCU for both

λ 400 and

λ500

respectively thus it is not acceptable

for the consumer to consume.
2. BOD Test
Table 6: Result of BOD test for Sample Water Before and After the Application of
Mudball Enzyme for a Period of 5 Days
Test
Before
After
BOD (mg/L)

BOD 0

BOD 5

BOD 0

BOD 5

3.3

3.0

3.6

3.5

Before Application of Mudball Enzyme,
Dilution Factor =

=
BOD

Bottle Volume (200 ml)
Sample Volume
200
300

=

0.667

= (Initial DO - DO5) / Dilution Factor
= (3.3-3.0) / 0.667
= 0.45 mg/L

After Application of Mudball Enzyme.667 = 0. COD Test Table 7: Result of COD test for Sample Water Before and After the Application of Mudball Enzyme Test Before After Value of COD (mg/L) 22 20 Table 8: DOE Water Quality Index Classification PARAMETER UNIT CLASS . However.45 mg/L. there won't be as many bacteria present to decompose the organic waste and thus the BOD will tend to be lower and the DO level will tend to be higher as shown in the results. From the result obtained. the BOD levels are high thus DO levels are low. after application of mud ball enzyme.6-3. the BOD level of lake water before application of mud ball enzyme are 0.15 mg/L which shows from the table above that the water quality are very good.DO5) / Dilution Factor = (3. Water from an exceptionally clear lake might show a BOD of less than 2 ml/L of water. This is because the demand for oxygen by the bacteria is high and they are taking that oxygen from the oxygen dissolved in the water. Generally.667 = (Initial DO .15 mg/L BOD test are conducted to evaluate the rate of oxygen uptake by micro-organism in the SST lake before and after the application of Mud ball Enzyme. however the BOD level of lake water after application of mud ball enzyme are 0. Bottle Volume (200 ml) Sample Volume Dilution Factor = 200 300 = BOD = 0.5) / 0. before application of mud ball enzyme. 3.

9)]/0. Thus. So the contaminants content was reduced after application of mud ball enzyme. The result of a chemical oxygen demand test indicates the amount of water-dissolved oxygen (expressed as milligrams per liter of water) consumed by the contaminants. TSS = ([A-B])/C = [(0. B (mg) Weight of suspended 154.3 = 3 mg/L Where A = End weight of the filter B = Initial weight of the filter C = Volume of water filtered After Application of Mudball Enzyme.8 158. A (mg) Weight of Filter Paper. Suspended Solid Test Table 9: Weight of Suspended Solid for Sample Water Before and After the Application of Mud ball Enzyme Test Before After Weight of Filter Paper + 155. the chemical oxygen V > 100 demand (COD) value for lake water before application of mud ball enzyme is 22 mg/L however the COD value of lake water after application of mud ball enzyme was reduced to 20mg/L.7 159.2 Suspended Solid.5 solid (mg) Before Application of Mudball Enzyme.9 0.3 = 1. 4. the COD of both water sample is in class II which indicate that conventional treatment are required for water supply.Chemical Oxygen mg/l I < 10 II 10 - III 25 - IV 50 - Demand 25 50 100 According to DOE Water Quality Index Classification. TSS = ([A-B])/C = [(0.7 0.67 mg/L .5)]/0.

. It is important to maintain or reduce the concentration of suspended solid in SST lake because high concentrations of suspended soils may settle out onto water bodies hence affect aquatic organisms. However. water with a SS concentration less than 20 mg/l is considered to be clear.67 mg/L after application of Mud ball Enzyme. This may produce coating hence preventing sufficient oxygen transfer and causing deaths of aquatic life (Weiner and Matthews. Water with SS levels between 40 and 80 mg/l tends to appear cloudy. 2006). none of the water samples from SST lake is considered to be cloudy or dirty. 2003). the concentration of Suspended Solid at SST lake was reduced from 3mg/L to 1. while water with concentrations over 150 mg/l usually appears dirty (Pepper et al. Nevertheless.Based on the results calculated above.