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WATER TREATMENT MICROBIOLOGY

INTRODUCTION
Some knowledge of the behavior of microorganisms In water 1s extremely important, since their presence can cause corrosion or plugging of equipment or the
injection weTlbore. They are simply another source of plugging solids or conditions which result in corrosion.
TERMINOLOGY
Biology can be defined as that branch of knowledge that deals with living
organisms and vital processes. Thus, in its broadest definition, it is that part
of science which is concerned with the life processes of plants and animals.
Microbiology is one branch of biology which concentrates on microscopic forms
of life known as microorganisms. Of primary concern to us is the behavior of microscopic, single-ceiled plants which are capable of living under a11 sorts of
conditions and multiply with incredible speed.
General Classes
The lowest or simplest segment of the plant kingdom of microorganisms can be
divided into three main groups:(l)
1. Algae - contain chlorophyll. They need sunlight to grow and can be a
. problem in surface ponds, open pits or seawater.
2. Fungi - do not contain chlorophyll. Fungi do not usually constitute a
major problem in injection operations.
3. Bacteria - have some properties in common with both fungi and algae.
Bacteria comprise the broad class of microorganisms of greatest interest
to us in water handling.
Size and Shape
Bacteria are extrsmely small (about 0.5 vm in diameter) and exist in literally thousands of species. True bacteria are shaped like spheres, straight rods,
or curved rods. In the language of the microbiotogist, the shapes are named as
follows:
1. A single spherical bacterium: coccus
Several spherical bacteria: cocci
As a matter of interest, a string or chain of cocci is called a
streptococcus, while a sheet or plane of cocci is called a staphlococcus.
2. Straight rod: bacillus
3. Curved rod:
Vibrio - slightly curved in the form of a "C" or an "S."
Spirillum -shaped like a screw or spiral.
Growth
The reason that bacteria can create so much trouble for us is that they can

multiply with incredible speed. They can double their population in 20 minutes
under many conditions, which means that a single bacterium can become a thriving
colony of millions of bacteria in a very few hours. A handful of slime from a
water may contain as many bacteria as there are people in the world.
Bacteria flourish under an extremely broad range of conditions, although they
grow-best in the- pH range of 5 to 9 and at temperatures of -18 to +80 C. They
prefer fresh water, but can do nicely in brines. Bacteria have been found in
packer fluids at 2500 m and in soil in the High Arctic. They are very hardy.
Oxygen Requirements
One method of classification of bacteria which is of interest in oilfield
systems is whether or not a specific bacteria requires oxygen to live. They fall
into three categories:
1. Aerobic bacteria - require oxygen to grow.
2. Anaerobic bacteria - grow best in the absence of oxygen.
3. Facultative bacteria - grow 1n either the presence or absence of oxygen
BACTERIA WHICH CAUSE PROBLEMS
Sulfate Reducing Bacteria
Sulfate reducers (Desulfovibrio Desulfuricans) probably cause more serious
problems in oilfield injection systems than any other bacteria. They reduce su1
fate ions in the water to sulfide ions, resulting in H.,S as a by-product.
Four types of problems can result from sulfate reduc^r activity in an injec'
tion system:
1. Pitting corrosion directly beneath a growing bacterial colony can occur
as shown in Figure 5.1.

Figure 5.1 Corrosion Fit Beneath


Sulfate Reducing Bacterial Colony
2. The generation of hLS by bacteria can increase the corrosivity of the
water. If the system is already sour, the additional HS generated by
the bacteria may have little or no effect. However, if the system was
originally sweet, the add-ition of hLS to the system by bacterial activity
can substantially increase corrosion rates and result in a pitting attack
throughout the system.
^
3. The presence of sulfate reducing bacteria in a system which was originally
free of H?S creates the possibility of sulfide clacking and blistering.
4. Sour corrosion results in the formation of insoluble iron sulfide which is
an excellent plugging material.
Su1fate reducing bacteria live in groups or colonies on the pipe wall, and
pits occur wherever they reside. Bacteria find it much easier to colonize on the
pipe vall than in a moving stream of fluid. Any time you find bacteria in the
water this means that there are many, many more securely attached to the waits
of the piping and tankage.
Sulfate reducing bacteria are most likely to be found in stagnant or "low velocity areas, and beneath scales or sludges. Common places for bacterial
activity

in injection systems are tanks, filters and the rat hole in injection and water
source wells.
Sulfate reducing bacteria are anaerobic bacteria. However, they are quite
capable of thriving ''n oxygenated systems, providing that they can find some
seal
or sludge to congregate under. Here they can usually find a sufficiently oxygenfree environment to live happy healthy lives. . Bacteria are very difficult to
kill if they are effectively shielded by scale or debris.
Sutfate reducers are also found in oxygenated surface waters such as oceans.
They are usually present in very small numbers and are not multiplying at an appreciable rate. However, if the water is deaerated prior to injection, they ther
have an oxygen-free en'/ironment which is ideal for their growth since they are
anaerobic. Sulfate reducing bacteria"! activity is a common occurrence 1n deaerated seawater injection systems.
Iron Bacteria
Iron bacteria deposit a sheath of ferric hydroxide around them as they grow
The iron is obtained from soluble iron ions in the water. Examples of iron bacteria are Siderocapsa, GaTlioneUa, Sphaerotilus and Crenothrix. They are classified as aerobic bacteria, although they can apparently grow well with only tra
amounts of oxygen.
Iron bacteria can cause
directly participate in
the activity of sulfate
an oxygen concentration

both corrosion and plugging. Although they do not


the corrosion reaction, corrosion can result either from
reducers under the hydroxide sheath or by the creation o
cell.

Large numbers of iron bacteria can precipitate a sufficient quantity of fer


ric hydroxide to cause severe plugging problems.
Iron bacteria are usually found in fresh waters but they may occur in brine.
Slime Formers
Slime forming bacteria are a general class of bacteria capable of producing
dense masses of slime on solid surfaces. Examples are Pseudomonas,
Flavobactenum,
Aerobacter, Escherichia, and Bacillus. They are magnificent pluggers and contribute to corrosion in the same ways as iron bacteria by shielding part of the
surface. Slime can be expected in either brine or fresh water systems, although
they
are more common in waters of tow salinity.
Slime forming bacteria may be found in either aerobic or anaerobic systems.
However, they are more generally a problem in aerobic systems.
Others
There are many other types of bacteria present in water injection systems
which are not discussed here. The sulfate reducers, iron b^c-'ceria and slime
formers constitute the major source of microbiological problems, and some knowledge of their behavior will keep you out of trouble the majority of the time.
. CULTURING, IDENTIFYING AND COUNTING BACTERIA
Monitoring a system for bacterial activity entails sampling (which is covered in the following section), identification and counting of the bacteria.
Microscopic Examination

A microbiologist can identify iron and slime bacteria using a microscope.


This technique does you little good unless you are trained to recognize them.
Cutturing Techniques
Culturing bacteria is analogous to cutturing flowers, potatoes, or green
beans. The object is to make them grow.
A water sample thought to contain bacteria is placed in a liquid known as a
"culture medium" which is simply a solution containing food that will make the
bacteria of "interest grow and multiply. Different types of bacteria require
different culture media, and some bacteria refuse to qrow at all in artificial
media.
Fortunately most bacteria of interest can be cultivated in a particular medium.
The -fact that me^ia can be formulated in which only specific types of bacteria
will grow makes it possible to identify the bacteria simply by noting the media
in which growth occurred. Furthermore, by running the sample at several different dilutions, the number of each type of bacteria can be estimated.
Laboratory Techniques. There are many different versions of laboratory identification and counting techniques, but they wi11 not be covered here. The general
principles already outlined are followed with varying degrees of sophistication.
API RP 38 describes a recommended method for examination of water flood
injection
waters and is used by many laboratories.
A Field Technique. A procedure known as the extinction dilution technique, or
serial dilution technique, which utilizes the media described in API RP 38 (2)
can be used in the field to culture water samples.
a. Extinction Dilution Technique (3)
The procedure is performed as follows:
(1) Line up a series of serum bottles containing 9 m& of sterile growth
medium.
(2) Inject 1 m& of the water sample into the first bottle and shake
well.
(3) Withdraw 1 m of solution from the first bottle with a disposable
sterile syringe and inject it into the second bottle. Shake the
bottle well. Throw the syringe away.
(4) withdraw 1 m of solution from the second bottle with a new sterile
syringe and inject it into the third bottle. Shake the bottle well
and throw the syringe away.
This procedure may be repeated for as many bottles as you wish and is
known as serial dilution. The point of this procedure is to dilute the
sample to the point that the final 1 m^ of solution that you inject into
the last bottle has no bacteria in it; hence, the name extinction dilution technique. You have diluted the sample to the point of extinction
of any bacteria present. It is illustrated on page l59.
The fact that a series of dilutions are performed allows you to estimate the bacterial population of the original 1 m& water sample.
b. Bacterial Growth Period

Once the bottles have been inocculated, they are set aside and
allowed to "incubate" for 30 days. A constant temperature is desirable during the incubation period and growth rates are temperature sensitive, API
RP 38 recommends that the cultures be incubated at a temperature within 5C
of the recorded temperature of the water at the time of sampling.
When culturing general bacteria using the API media, growth is indicated when the serum bottle becomes turbid or cloudy. Recent modifications
to the API media include the addition of phenol red, which is an acid-base
indicator. In this case, growth is indicated by either a color change from
red to yellow or_ the development of turbidity. The color change takes place
due to a reduction in pH caused by the production of organic acids by the
bacteria as they grow.(4)
Growth of sulfate reducing bacteria is indicated when the bottle turns
black. The media contains soluble ferrous iron. When sulfate reducers grow
they produce H^S which reacts with the iron to form black iron sulfide.
As a rule of thumb, growth will usually occur within three days for
general bacteria, but two weeks to a month may be required for sulfate reducer growth. The bottle should be examined daily for signs of growth and
the results should be recorded. The final reading for general bacteria
should be taken after seven days. The final count on the sulfate reducers
should be taken after 28 days.
c. Types of Cultures
In practice, two type's of culture media are utilized and,
two separate series of dilutions are run. The first series
tles which contain a growth medium for "general" bacteria.
utilizes 4 bottles which contain a different growth medium
to sutfate reducing bacteria.

therefore.
utilizes 7 bot- The second series
which is specific

The "general" count includes the general aerobic bacteria, primarily the
slime formers. It does not include iron bacteria, which are difficult to
culture in an artificial"medium. They are usually detected by microscopic
means.
The sutfate reducing bacteria count 1s specific, as previously stated.
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d. Interpretation of Results
Interpretation of results can best be -illustrated by example:
If bottles 1, 2 and 3 become turbid, but bottles 4 through 7 remain
clear, then the water contains 100-1000 bacteria per milliliter. If only
bottle number 1 becomes cloudy, and the rest remain clear, then the water
contains 1-10 bacteria per mi 11 ititer.
e. Significance of Results
(1) General Count
Bacterial counts of less than 10 000 organisms per ms, are not
considered significant in untreated waters. AS a rule of thumb, a count
of 100 000 per nu indicates a strong possibility of plugging and a need
for biocide treatment. Start examining injectivity for any decrease and
look for any evidence of increased injection pressures or filter plugging.
Some slime formers will only grow on c solid surface and will not

be detected by this technique.


(2) - Sulfate Reducing Bacteria
A single sulfate reducer is a sign of trouble. For every one
which you find in the water, many of its relatives are growing on the
pipewall in a colony or colonies somewhere in the system. This is the
reason that only 4 bottles are run. Any number of sulfate reducers is
a potential problem.
Cu1turi'ng_Sca1e. ^Bacteria usually prefer to grow on the wall of the pipe, or
bet
ter yet (in the case of sulfate reducers), under some scale or debris. Because o
this, it is often desirable to scrape some scale or solids from the pipe wall
and
culture it in one or more liquid media.
The bottles used for API water culturing technique cannot be used because
they have rubber septums covering their necks and are designed for use with syringes. Therefore, sterilized bottles with screw-on caps must be obtained contain
ing the desired growth media. A consulting laboratory, or a chemical company,
should be contacted for help in the event you should want to culture scale
sample
It is highly recommended when there are signs of bacterial activity in the
system
yet conventional culturing of water samples fails to show the presence of
bacteria
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ATP Analysis.(5)
ATP analysts provides a means for rap-idly determining the quantity of living
organisms present in an injection water. It is not specific to bacteria, but
since the majority of the organisms present in many oilfield waters are
bacteria,
-it can be a very useful technique. It is particularly helpful in the evaluation
of biocide treatment programs and is being increasingly used in the oil
industry.
The basis for the method is as follows:
1. ATP (adenosine triphosphate) is present in the cells of all living
organisms, both plant and animal.
2. ATP is destroyed within 20 seconds of cell death through release of
ATP-destroying enzymes.
3. The amount of ATP per cell is a linear function of celt volume, e.g.,
twice the cell volume results in twice as much ATP.
ATP analysis is a method of measuring the amount of ATP present in a given
sample. This enables a quantitative measurement of the amount of living organisms in the sample. The method is based on the fact that ATP wi'1'1 react with a
mixture of two enzymes, "luciferin and luciferase in the presence of oxygen to
produce light (bioluninescence). The amount of tight emitted from the reaction
is measured with a photometer and the number of organisms determined from calibration data.
A summary of the procedure is as follows:
1. The water sample is filtered through a 0,45 urn pore size membrane filter.
Bacteria and other organisms are thus removed from the water and retained

on the filter surface. This concentrates the organisms, thus increasing


measurement sensitivity. Also, it 1s required when the water contains
NaCI (as in the case of most oilfield waters) because NaCI interferes
with the ATP-tuciferin/luciferase reaction.
2. The ATP must then be quickly extracted from the cells prior to its destruction by enzymes soon after celt death. Several methods are available. but a common method is to drop the membrane filter into a boiling
solution of 0.05 molar tris hydroxy nitromethane buffer solution. The
heat destroys the ATP-destroying enzymes, preventing their interference.
It also kills the bacteria and causes them to burst and release their
cellular contents, including ATP, into the buffer solution.
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3. Luciferin/luciferase is then added to the buffer solution and the emitted
light .measured with a photometer. The number of bacteria present is then
obtained from calibration data.
ATP analysis has one overwhelmingly positive feature: it provides a very
quick indication of the total bacterial activity in a system. You don't have to
wait through long incubation periods to get results as in the case of
conventional
culturing techniques. However, it does not identify the type of bacteria
present.
, As previously stated, the presence of a few sulfate reducing bacteria can
cause trouble, whereas a general count of 10 000 bacteria per ma can usually be
tolerated. Therefore, if ATP analysis were to indicate a total count of 5000
bacteria per nu in a particular sample, this would be acceptable providing none
were
sulfate reducers. However, the situation would be quite different if 2000 of
those bacteria were sulfate reducing bacteria; Because of the need to
specifically identify sulfate reducers. it "is common to use conventional culturing techniques to determine their population in conjunction with ATP analysis as a rapid
means of measuring total bacteria"! activity.
Mater Sampling for Bacterial Analysis
1. Sampling Points
Sample the following points in an injection system:
a. Water Source
The wellhead, pond, stream, lake, ocean, or produced water.
b. Vessels, Tanks and Filters
Sample both the inlet and outlet, as well as the backwash discharge on filters.
c. Injection Wells
Sample at several injection wellheads located at various distances froiii the injection plant.
2. Sampling Technique
The sampling technique is basically the same as described in an earlier chapter. There is one additional requirement. The sample container
must initially be sterile, or free of bacteria. Additionally, don't use
a plastic or rubber sampling hose as it is likely to be contaminated. You

don't want to contaminate the sample and find a bacterial problem which
doesn't really exist in the system.
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An excellent sterile sample container 1s a new prescription bottle which
can be obtained at any drugstore.
3. Timing
The water sample should be injected into the growth media or subjected to ATP analysis immediately after sampling if at aH possible.
Changes in the bacterial population occur rapidly and old samples can
give a false picture of the population in the water system.
It is recommended that samples be cultured no longer than 8 hours
after sampling. If you are performing the analysis yourself, or if someone else is going to do it on-site, this poses no problem. However, if
you are shipping a sample to a laboratory for bacterial analysis, keep
the 8-hour time limit in mind.
4. Sulfide Concentration
Sniff the sample for hLS, then measure the total sulfide concentration. If the water entering the system is sweet, but becomes sour somewhere m the 'system, this is a very good sign that sulfate reducers are
generating H?S in the system.
Also note the color, clarity and amount of suspended solids. Severe
sulfate reducers problems sometimes cause the water to turn black due to
the formation of iron sulfide.
Algae

MISCELLANEOUS ORGANISMS

Algae growth can be a problem in open systems where pits or open tanks are
used for storage. Algae are single celled plants which contain chlorophyll and
require sunlight for their growth. They can occur in any type of fresh or salt
water.
Algae will form slime on the water surface which is easily identified by its
green or blue-green color. It is often observed in stagnant water and in cooling
towers.
Algae can be mucked into a water injection system and cause plugging. If they
completely blanket a pond or pit, anaerobic conditions conducive to the growth
of sulfate reducing bacteria can occur in the water.
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Algae are present in seawater, although usually not as slimes. There are
significant numbers in most oceans and they can contribute substantially to the
plugging solids in the water, especially during algae "blooms" which occur at
certain times of the year.
Protozoa
Protozoa are the simplest form of animal life. They are found in both fresh
and salt water and require oxygen to live. In water injection systems, they are
found in open tanks or pits. They are also found in filters when the water 1s
aerated. Generally, control of the other microbial population suffices to
control
the protozoa population.
Plankton

. The oceans of the world contain creatures which are referred to as plankton.
The term plankton is applied to all those animals and plants which live freely
1n
the water and which, because of their limited powers of movement just drift
along
with the water currents. This is not to say that they cannot swim. Some, such
as Crustacea and fish larvae, can swim very well but they may not be able to
swim
faster than the water moves,
Plankton may be grouped by sizes. Very large animals such as jellyfish are
called megatoplankton. Those organisms which are readily visible w""th the naked
eye and down to a size which is retained by a net with 1 mm holes are referred
to
as "course net" or macroplankton. Organisms smaller than 1 mm in size, but
larger
than 75 pm are microplankton. The smallest size is called nanoptankton. It includes tiny plants, bacteria, and creatures called flagellates. The smallest
flagellates,that is those below 5 microns* are sometimes called ultraplankton.
Plankton are important to us because they make up a significant portion of
the plugging solids in most seawaters. Also, they serve as food for larger creatures which may in turn contribute to plugging.
CHEMICAL CONTROL OF MICROORGANISMS
Types of Chemicals
Chemicals used for bacterial control can be broadly classed in several ways.
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1. Function
a. Bactericide
A chemical which kills bacteria.
b. Bacteriostat
A chemicel which inhibits or retards the growth of bacteria.
c. Biocide
A chemical which kills other -Forms of life in addition to
bacteria. A more universal killer.
d. BIOS tat
A chemical which retards or inhibits the growth of other life
forms in addition to bacteria.
2. Chemical Composition
a. Inorganic Chemicals
Chlorine is the most widely used inorganic biocide for water injection systems. Chromates and compounds of mercury and silver
have bacteriddal qualities, but are seldom used in injection
systems.
b. Organic Chemicals
Amines, chlorinated phenols and quaternary ammonium compounds are

common examples of organic bactericides. Most bactericides sold


by chemical companies, other than chlorine, are organic.
Chemical Selection and Evaluation
Chemical selection for bacterial control is analogous to the selection of a
chemical for the control of corrosion or scale.
First the problem (the type of bacterial problem) must be identified, then
chemicals chosen which will deal with it effectively. Laboratory evaluation of
different bactericides is recommended as an ititial step in chemical selection.
Several items must be considered in selection:
Ki11 or Control? The decision whether to use a bactericide or a bacteriostat 1s
dictated by the type of bacteria involved. Sulfate reducers require a bactericide, since a total kill is desired. Since a reasonable number of slime formers
can be tolerated without serious problems, a bacteriostat is often used for
their control.
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Resistant Strains. Bacteria have a very discouraging habit of developing strains
which build up a resistance to a particular chemical over a period of time. They
then proceed to multiply and cause problems in spite of the fact that you are
religiously adding a bactericide to the system. Therefore, it is good practice to
select a minimum of two bactericides and alternate their application. Then. if a
strain begins to develop which is resistant to the first chemical, <\ change to
the
second chemical will take care of the problem.
Compatibility with Water and Other Chemicals. Make sure that the chemical is
compatible with your water. Some will precipitate or "salt out" in higher
brines.
Also, compatibility with corrosion or scale inhibitors must be established.
Time-Kill Tests. It takes time for any bactericide to kill the bacteria present.
Therefore, it is wise to determine what length of time is necessary for a
particular chemical to do its job at several chemical concentrations. This must
be per-formed by a laboratory. A specific method for sulfate reducers is spelled
out in API RP 38.
It is important to know the time-kill relationship, especially where batch
treatment is utilized.
Treatment Method. The treatment method must also be considered. If the bactericide is to be continuously injected an initially high concentration is usually
required to bring the bacterial population under control, then lower chemical
concentrations can usually be effective once the bacterial population has been
brought
under control. This minimum can be estimated from laboratory tests.
If batch treatment is to be used, then a high concentration slug is periodically pumped through the system, and the object is a total kill. Here the
concentration, slug size and contact time can be initially sized by time-kill tests,
then adjusted by actual experience.
Field Evaluation. As in any other situation, the ultimate question is whether or
not the chemical(s) selected actually works in your system. This can only be determined by closely monitoring the system.

a. Make sure the bactericide is actually going into the system at the proper
concentration.
b. Monitor the number of bacteria present as a function of time.
Chemical Application
Clean the System. The first rule of successful use of bactericides is to clean
the system.
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a. Clean out the injection lines and tubing as outlined in Chapter 3, using
solvents, acid and line scrapers if necessary.
b. Open a11 tanks, vessels and fitters, and manually clean out all accumulated sludge and scale.
c. Backftow all injection wens. Acidize if necessary. If the wellbore is
plugged with microbiological mass or slime, a water solution containing
chlorinated lime or calcium hypochlorite may be used as a soak prior to
backftow. This can be a tremendous help in weTlbore cteanout and is
available from service companies for this purpose.
A thorough cleaning job is necessary and has only one purpose: to remove
a11 obstacles between the bactericide and the bacteria. No chemical can kill
bacteria if it cannot contact themi(6)
The same thing is true in the use of corrosion inhibitors. It is very difficult to inhibit the corrosion reaction unless the inhibitor can contact the
point where the corrosion is occurring.
Of course, bactericides, tike corrosion inhibitors, contain a certain amount
of detergency. If the system is not too dirty, there may be enough detergency to
clean up the system. However, this is seldom true and a systematic clean.ing operation prior to initiation of treatment is strongly recommended.
Also, remember that if you rely strictly on the detergency of the chemical,
try to prevent the solids which may be washed loose from being injected into the
formation. This can be accomplished by installation of wellhead cartridge
filters
Eliminate Stagnant or Low Velocity Areas. Examine the possibility of eliminating
stagnant or low velocity areas by changes 'in system design. Since bacteria grow
more rapidly in slowly moving or stagnant waters, system modification to
minimize
the number of tow velocity areas will make bacterial control an easier task.
Sterilize the System. Once the system has been cleaned, it should be
"sterilized.
This means that the bacteria must now be killed. A high concentration slug of
bactericide, with sufficient residence time to kill the bacteria is used. Don't
forget that this means the tines, tanks, filters and injection wells. However,
it also neans the water source. This frequently involves producing wells if produced water is used.
Institute Treatment. At this point "normal'' treatment, either continuous or
batch, can be instituted. The chemical should be added as near to the water
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Figure 5.2 Effect of pH on the


Concentration of Hypochlorous Acid
(From Ref. 7)
Some typical data:
pH

Chlorine Residual Required


for 10 Minute Kill (ppm)

6-8

0.2

8-9

0.4

9-10

0.8

The chlorine residual or "free" chlorine 1s the total amount of chlorine present
as C1?, HOC1, and OCI".
Since chlorine is a very strong oxidizing agent, it reacts with many materials. Once it reactSs it is no longer available to km bacteria. Hence, it i
necessary to establish how much chlorine will be used up by reaction with other
materials in order to establish the total amount of chlorine needed. The amount
used up by the system is called the chlorine demand. The amount of chlorine
which must be injected to kill bacteria is:
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CHLORINE REQUIRED
FOR BACTERIAL
= TOTAL CHLORINE
CHLORINE
CONTROL
INJECTED
DEMAND
Some of the things which chlorine reacts with are:
(1) Ferrous Iron
One ppm chlorine will oxidize 1.6 ppm ferrous iron
(Fe++) to ferric iron (Fe+++),
(2) Hydrogen Sutfide
Chlorine win react with hLS as previously mentioned in
Chapter 4.
(3) Organic Compounds
Chlorine win react with some corrosion inhibitors and scale
control chemicals.
(4) Sulfite Ion
Chlorine will react with the sulfite oxygen scavengers or with
sulfur dioxide. This was also covered in Chapter 4.
AH of this points up the fact that the use of ch'iorine is limited if there is a
lot of H?S or ferrous iron to cope with in the system.
Chlorine is not normally used in systems which have long, bare steel injection lines. If the line is several miles long, it will be necessary to inject

large quantities of chlorine in order to obtain a residual of 0.2-0.5 ppm at the


end of the line due to the loss of chlorine by reaction with the steel. This can
result in increased corrosion rates as well as increased treatment costs.
The compatibility with any other chemicals to be used in the system must als
be checked. If oxygen scavengers are used, either of two approaches may be followed:
1. The chlorine may be added a sufficient distance downstream to permit the
oxygen scavenging reaction to go to completion prior to reaching the
chlorine injection point.
2. If chlorine is to be injected upstream of oxygen scavenger injection.
additional scavenger must be injected so that there will be enough present to react with both the chlorine and the oxygen. Since this results
in removal of the chlorine, it is necessary to inject chlorine or some
other biocide downstream of oxygen scavenging if a biocide is required
in the downstream portion of the system.
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Chlorine 1s usually added in gaseous form from steel cylinders. The rate of
addition is controlled with chlorinator. A typical vacuum operated, solution
feec
chlorinator is shown in Figure 5.3.

Figure 5.3 Flow Diagram of


Solution Feed Chlorinator
Mater flowing through the ejector at high velocity creates a vacuum which opens
a spring-opposed diaphragm check valve in the ejector body. when the check va1
opens, a vacuum is transmitted to the vacuum regulator, causing the diaphragm t
open the chlorine inlet valve. Chlorine gas passes through the flow meter and
rate control valve to ,the ejector, where it mixes and dissolves in the water.
Caution: Chlorine is very poisoneous and must be handled with care.
Hypochlorite (OCI") can be produced in chloride containing waters by electrolysis. The brine -is passed through a special electrolytic cell called a hyF
chlorite generator, which contains two electrodes, an anode and a cathode. An
impressed DC electrical current flows from the anode to the cathode, through tl
brine. Hydrogen ions are'converted to hydrogen gas at the cathode and chlorid'
ions are converted to OC1" ions at the anode as shown in Figure 5.4.
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Figure 5*4 Chemical Reactions in a Hypochlorite Generating Cell


The OC1~ solution/hL gas mixture leaving the generator is usually passed

through a gas-water separator to separate the hydrogen gas from the hypochtorite
solution prior to its addition to the water system. The hydrogen is separated
and vented for safety reasons, since the lower limit of f1ammab11ity for
hydrogen
in air is 4.1%.(8)
Hypochlorite generators are commonly used in seawater systems to eliminate
the need for transporting and storing liquid chlorine.
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