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SAFETY IN THE IMMUNOLOGY

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SEROLOGY LABORATORY

OUTLINE
I. SAFETY REGULATIONS AND PRACTICES
II. PREVENTION OF TRANSMISSION OF
INFECTIOUS DISEASES
III. SAFE WORK PRACTICES, PROTECTIVE
TECHNIQUES, AND HANDWASHING
IV. DECONTAMINATION OF WORK SURFACES,
EQUIPMENT, AND SPILLS
V. DISPOSAL OF INFECTIOUS LAB WASTE

Due to carelessness or lack of proper communication • Safety .should be uppermost in the mind of any worker .• Accidents .

Agencies or Committees – Occupational Safety and Health Administration (OSHA) – Clinical and Laboratory Standards Institute (CLSI) – Centers for Disease Control and Prevention (CDC) – College of American Pathologists (CAP) – The Joint Commission (TJC) .

Wearing protective equipments ii. Proper centrifugation iv. Pipetting techniques . Food and drinks iii. Proper way of uncapping test tubes v. SAFETY REGULATIONS AND PRACTICES • Safety Manual – To minimize the risks to health and safety i.

OUTLINE I. AND HANDWASHING IV. AND SPILLS V. PROTECTIVE TECHNIQUES. SAFE WORK PRACTICES. EQUIPMENT. DECONTAMINATION OF WORK SURFACES. DISPOSAL OF INFECTIOUS LAB WASTE . PREVENTION OF TRANSMISSION OF INFECTIOUS DISEASES III. SAFETY REGULATIONS AND PRACTICES II.

HBV. and other bld-borne microorganism . PREVENTION OF TRANSMISSION OF INFECTIOUS DISEASES • Standard Precautions – All human blood and other body fluids • Potentially infectious for HIV.

PREVENTION OF TRANSMISSION OF INFECTIOUS DISEASES – Direct transmission • Percutaneous inoculation • Contamination of skin • Exposure of mucous membranes • Droplet formations .

SAFE WORK PRACTICES. AND HANDWASHING IV. AND SPILLS V. DISPOSAL OF INFECTIOUS LAB WASTE . DECONTAMINATION OF WORK SURFACES. SAFETY REGULATIONS AND PRACTICES II. PREVENTION OF TRANSMISSION OF INFECTIOUS DISEASES III. PROTECTIVE TECHNIQUES. EQUIPMENT. OUTLINE I.

PROTECTIVE TECHNIQUES AND HANDWASHING • PPE Program (OSHA) – Hazard assessment – Proper equipments – Employee competency • Protective Techniques – Proper gloves selection – Use of masks and facial protection – Lab coats or gowns . SAFE WORK PRACTICES.

high intensity lighting.overexposure to harmful dusts. electrical connections and sharp edges • Health hazards . rolling or pinching objects.moving objects. Hazard assessment • The first critical step in developing a comprehensive safety and health program is to identify physical and health hazards in the workplace • Physical hazards . fluctuating temperatures. chemicals or radiation .

adjust and wear the PPE? • Disposal of PPE . take off. and should be maintained in a clean and reliable fashion • When PPE is necessary? • What PPE is necessary? • How to properly put on. Proper equipments • All PPE clothing and equipment should be of safe design and construction.

Employee competency • Qualifications • Trainings and Workshop • Seminars .

PROTECTIVE TECHNIQUES AND HANDWASHING .SAFE WORK PRACTICES.

Before. after lab work and before leaving the lab ii. and before and after bathroom usage iv. SAFE WORK PRACTICES. or hand antiseptics i. After removing gloves iii. Before eating. PROTECTIVE TECHNIQUES AND HANDWASHING • Handwashing – Soap and water. After accidental skin contact with specimens . drinking.

DISEASE PREVENTION . SAFETY REGULATIONS AND PRACTICES II. PROTECTIVE TECHNIQUES. AND HANDWASHING IV. SAFE WORK PRACTICES. DISPOSAL OF INFECTIOUS LAB WASTE VI. OUTLINE I. DECONTAMINATION OF WORK SURFACES. PREVENTION OF TRANSMISSION OF INFECTIOUS DISEASES III. EQUIPMENT. AND SPILLS V.

AND SPILLS • Sodium hypocholorite – Household chlorine bleach (by EPA) • Environment Protection Agency • 1:5 effective for biohazardous spill clean up. DECONTAMINATION OF WORK SURFACES. • 1:10 effective surface decontaminant (if surface can stand it). • 1:100 commonly used for routine wipe down of surfaces. . EQUIPMENT.

AND SPILLS • Spillage 1. DECONTAMINATION OF WORK SURFACES. Place the disposable materials in biohazard container . Clean spill site w/ 1:10 diluted bleach using paper towel 4. Absorb blood w/ disposable towels 3. EQUIPMENT. Wear gloves and lab coat 2.

PROTECTIVE TECHNIQUES. SAFE WORK PRACTICES. AND SPILLS V. SAFETY REGULATIONS AND PRACTICES II. DISEASE PREVENTION . AND HANDWASHING IV. EQUIPMENT. OUTLINE I. PREVENTION OF TRANSMISSION OF INFECTIOUS DISEASES III. DISPOSAL OF INFECTIOUS LABORATORY WASTE VI. DECONTAMINATION OF WORK SURFACES.

pathologic wastes. and microbiologic wastes (by OSHA) – Containers for Waste • Biohazard containers – Leakproof and secure lids (biohazard sign) • Biohazard bags – w/ biohazard sign . contaminated sharps. DISPOSAL OF INFECTIOUS LABORATORY WASTE • Infectious waste – Blood and blood products.

wet stuff • Sharps – needles. gloves gowns and other non sharps • Liquids . used glassware pipettes and pipette tips . Common Types of Biological Waste • Solids – sample tubes.

DISPOSAL OF INFECTIOUS LABORATORY WASTE .

DISPOSAL OF INFECTIOUS LABORATORY WASTE .

DISPOSAL OF INFECTIOUS LABORATORY WASTE General Guidelines • Segregate Biological from Chemical waste • NO sink disposal of chemicals • Use clear autoclave bags for biological hazardous waste .