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Kittikhom Wannapak(Pong

)
Keawalin Sirirattanaprasert(Fah)
Napatsorn Supreyasunthorn(Maple)
Sep 18​th​, 2015
11- 5
Experiment : Chromatography paper Lab

Abstract​: In this experiment is to use thin chromatography paper to make solution seperate
into group by dropping 6 sample on the paper. Then allow the solution to seep the way up on
paper​. ​Gather all observation in experiment and it came up with the color seperated into
different location.

Purpose​: To seperate and identification of the mixture of sudstance of the solution. We will
see what are the mixture of two unknown samples by testing with for samples, using
Chromotography paper.

Introduction:​ This is some background of Chromotrography paper, ​the Russian botanist
Mikhail Tswett used column of calcium carbonate to produce a separation of plant pigments.
the purpose of Chromatography is for separation and identification of compounds. They
have many kind of mixture depends on the methods that using. For an example, T ​ hin Layer
Chromatography. In this experiment, we will use Chromotography paper to seperate the
mixture of substances into their components.

Data/Results

Figure1: The distance of sample travell in the lab.

Table 1. Rf values of the sample ans unknown sample
Calculation of Rf value:
Rf = ​distance from baseline travelled by solute
distance from baseline travelled by solvent

Substance ​​ Substance Solvent Rf values
distance(cm.) distance (cm.)

Sample1 2.0 7.8 0.26

Sample2 2.3 7.7 0.30

Sample3 2.9 7.8 0.37

Sample4 6.0 8.0 0.75

Unknown1 2.3 8.0 0.29

Unknown2 2.7 8.5 0.32

Sample1 = 2.0/7.8 = 0.26
Sample2 = 2.3/7.7 = 0.30
Sample3 = 2.9/7.8 = 0.37
Sample4 = 6.0/8.0 = 0.75
Unknown1 = 2.3/8.0 = 0.29
Unknown2 = 2.7/8.5 = 0.32

Discussion ​: According to the experient, each sample have different reaction to water, as
components of the mixtures ​has travelled at differant rate of distance. Also th mixture
seperated to be a layer of color. Chromotrography can seperate each sample into its
components as the mixture absorb the solvent, the it start to seperate into a layer of color. In
this experiment the spot must be placed be above the level of the developing solvent
bacause if the spot lower than that it will dissolve together with the solvent. So the
experiment won’t happen, the spot won’t travelled up. As each ink travell in different distance
so each Rf value is not equal. To calculate the Rf value, we use distance of substance
compare with distance of solvent. For example, If the solvent moves 8.0 cm and a
component of the sample moves 3.2 cm from the baseline. So the Rf value is 8.0/3.2= 0.40.

Conclusion:​ ​Sample 4 has the most different substance and sample 1 has the least
different substance.

References
- How TLC Works. (n.d.). Retrieved September 21, 2015.
- Methods for separating mixtures. (n.d.). Retrieved September 21, 2015.
- Desai, M., & Armstrong, D. (n.d.). Separation, Identification, and
Characterization of Microorganisms by Capillary Electrophoresis. Retrieved
September 21, 2015.