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CURRICULUM VITAE (abbreviated)

23 March 2017

MARCELO GONZLEZ

First degree (2004) Biochemist, Universidad de Concepcin, Chile


Postgraduate (2007) MSc (Biological Sciences), P. Universidad Catlica, Chile
Postgraduate (2009) PhD (Physiological Sciences), P. Universidad Catlica, Chile

Actual position (from 6/2009) Assistant Professor, Department of Physiology, Faculty of


Biological Sciences, Universidad de Concepcin, Concepcin,
Chile.

Research aim

My main interest is focused on elucidating mechanisms regulating vascular tone and deterioration
suffered by these mechanisms when tissues are exposed to conditions that produce oxidative stress in
normal or pathological conditions. My experimental approach seeks to explain pathophysiological
phenomena from molecular and cellular levels. Therefore, our students are training in classical
techniques of vascular physiology (wire myography, placenta perfusion), cell culture (human
endothelium and smooth muscle cells), biophysics (membrane transport), molecular biology (RT
-PCR, qPCR, promoter cloning, transfection), biochemistry (western blot, cell fractionation, etc.).
Actually, one of my objectives is the identification of mechanism and biomarkers for diagnosis and/or
treatment of cardiovascular disease associated with the development of obstetric pathologies such as
gestational diabetes mellitus or preeclampsia. Also my findings can be extrapolates for understanding
chronic diseases with high incidence in world population, such as diabetes mellitus, hypertension and
atherosclerosis.

Research Group (at present)

Students and support personnel


MSc: Andrea Saavedra, Victor Durn (MSc in Human Physiology, University of Concepcin).
Undergraduate: Natalia Vallejos, Emerita Basualto, Jos Merino, Walther Ide (Biochemistry, U.
Concepcin).
Research assistant: Susana Rojas.

Associate researchers
Carlos Escudero (U. Biobo), Victoria Gallardo, Claudio Aguayo, Katherina Fernndez, Mara
Mondaca, Marcela Cid (U. Concepcin), Enrique Guzmn-Gutirrez, I Rodrguez (U. San Sebastin),
Marcelo Faras, Luis Sobrevia (P.U. Catlica), Leandro Ziga (U. Talca).

Clinician associate researchers


Marcela Cid (U. Concepcin)

International Collaboration (at present)


Mark Wareing (Maternal and Fetal Health Research Centre, Institute of Human Development,
University of Manchester, Manchester, UK).
Thesis Supervisor
MSc: Pa vila, Lissette Cabrera (MScRes in Physiological Sciences, Faculty of Biological Sciences,
University of Concepcin), Ivn Rodrguez, Tamara Ortiz, Sebastin Dub (MSc in Human
Physiology, Faculty of Biological Sciences, University of Concepcin).

Undergraduate: Astrid Haensgen, Andrea Saavedra, Roberto Villalobos, Carlos Palma, Cristina
Valenzuela (Biochemistry, Faculty of Pharmacy, U. Concepcin), Pamela Careaga, David Gallegos,
Rubn Grandn (Bioengineering, Faculty of Biological Sciences, U. Concepcin).

Teaching: General physiology, cardiovascular physiology, signal transduction, reproduction,


endocrinology, plasma membrane physiology for undergraduate (Biochemistry, Medicine, Nutrition,
Chemistry & Pharmacy, Nursery, Obstetrics and perinatology, Kinesiology, Odontology, Medical
Technology, Bioengineering) and postgraduate (PhD in Molecular Biology, MSc in Biological
Sciences, MSc in Reproductive Health, MSc in Clinical Biochemistry). Topics: gestational diabetes,
fetal vascular dysfunction, cardiovascular pathophysiology, endothelial physiology, cell signalling.

Full member of Scientific Societies


UK: The Physiological Society. Chile: Chilean Society of Physiological Sciences (SCHCF), Chilean
Society of Pharmacology (SCHF).

Academic/Scientific Merit Recognitions


2013: Academic Assignation, University of Concepcin, Chile. 2013-2015: Elected Board Member of
Chilean Society of Physiological Sciences.

Executive Committees Membership


Chile: Scienfic committee coordinator of the First Meeting of Innovation and Research of Vascular
Health Scientific Committee (Congress 2015); Member of Academic Committee of Obstetrics &
Childcare Undergraduate Career (U. Concepcin, from 2013).

Invited Speaker (selected)


Chile: P.U. Catlica, U. Biobo, Chilean Society of Physiological Sciences. UK: The Physiological
Society, Maternal and Fetal Health Research Centre, Institute of Human Development, University of
Manchester. Austria: Medical University of Graz.

Research Visitor
UK: Kings College London (2003), University of Manchester (2011). Australia: Baker Research
Institute at U Melbourne (2008).

Reviewer for Scientific Journals


Biological Research, Frontiers in Pharmacology, Frontiers in Physiology, Placenta.

Associated Editor for Scientific Journals


Frontiers in Physiology

National and International Congresses (selected from 2008)


2016. Argentina: IADPSG 2016. Chile: SChCF. 2015. Argentina: SLIMP 2015. Chile: XXX
Reunin de la Sociedad Chilena de Ciencias Fisiolgicas (SChCF). 2014. Brazil: 1st Panamerican
Congress of Physiological Sciences. Chile: International Post Graduate Summer School-UDEC,
International Postgraduate Workshop (IPW) 2014. 2013. UK: 37th Congress of International Union of
Physiological Sciences (IUPS). Chile: SChCF, SCHF, IPW 2013. 2012. UK: The Physiological
Society. Switzerland: International Society for the Study of Hypertension in Pregnancy (ISSHP)
World Congress. Chile: SCHCF, IPW 2012. 2011. UK: The Physiological Society. Chile: SCHCF,
IPW 2011. 2010. UK: The Physiological Society. Chile: SCHCF, IPW 2011, International Federation
of Placenta Associations (IFPA), 1st Meeting of Free Radicals and Antioxidants Group. 2009. Chile:
SCHF, IPW 2009, Chilean Society of Biochemistry and Molecular Biology, II Latin American
Meeting of Physiological Sciences. 2008. Chile: SCHCF, Chilean Society of Biochemistry and
Molecular Biology.

Publications

1. Cabrera L, Saavedra A, Rojas S, Cid M, Valenzuela C, Gallegos D, Careaga P, Basualto E,


Haensgen A, Pea E, Rivas C, Vera JC, Gallardo V, Ziga L, Escudero C, Sobrevia L, Wareing
M, Gonzlez M. (2016). Insulin induces relaxation and decreases hydrogen peroxide-induced
vasoconstriction in human placental vascular bed in a mechanism mediated by calcium-activated
potassium channels and L-arginine/nitric oxide pathways. Frontiers in Physiology. Aceptado.
2. Dub S, Gallegos D, Cabrera L, Sobrevia L, Ziga L, Gonzlez M. (2016). Cardiovascular
action of insulin in health and disease: focus in endothelial L-arginine transport and cardiac
voltage-dependent potassium channels. Front Physiol. 7: 74
3. Sobrevia L, Salsoso R, Fuenzalida B, Barros E, Toledo L, Silva L, Pizarro C, Subiabre M,
Villalobos R, Araos J, Toledo F, Gonzlez M, Gutirrez J, Faras M, Chiarello DI, Pardo F, Leiva
A. (2016). Insulin Is a Key Modulator of Fetoplacental Endothelium Metabolic Disturbances in
Gestational Diabetes Mellitus. Front Physiol. 7: 119.
4. Lightfoot S, Bouza R, Prez M, Haensgen A, Gonzlez M, Rojas C, Rodrguez-Llamazares S.
(2016). Synthesis and characterization of polyhydroxybutyrate-co-valerate nanoparticles for
encapsulation of quercetin. J Bioact Compat Polym. 31(5): 439-452
5. Cid M, Gonzlez M. (2016). Potential benefits of physical activity during pregnancy for the
reduction of gestational diabetes prevalence and oxidative stress. Early Hum Dev. 94: 57-62.
6. Rodrguez I, Romero F, Gonzlez M, Ribeiro de Campo R. (2015). Biologa del desarrollo
vascular: Mecanismos en condiciones fisiolgicas y estrs de flujo. Int J Morphol 33: 1348-1354.
7. Gonzlez M, Rojas S, Avila P, Cabrera L, Villalobos R, Palma C, Aguayo C, Pea E, Gallardo V,
Guzmn-Gutirrez E, Sez T, Salsoso R, Sanhueza C, Pardo F, Leiva A, Sobrevia L. (2015).
Insulin reverses D-glucose-increased nitric oxide and reactive oxygen species generation in
human umbilical vein endothelial cells. PLoS One. 10(4):e0122398.
8. Rodrguez I, Gonzlez M. (2014). Physiological mechanisms of vascular response induced by
shear stress and effect of exercise in systemic and placental circulation. Frontiers Pharmacol.
5:209.
9. Campos J, Schmeda-Hirschmann G, Leiva E, Guzmn L, Orrego R, Fernndez P, Gonzlez M,
Radojkovic C, Zuiga FA, Lamperti L, Pastene E, Aguayo C. (2014). Lemon grass (Cymbopogon
citratus (D.C) Stapf) polyphenols protect human umbilical vein endothelial cell (HUVECs) from
oxidative damage induced by high glucose, hydrogen peroxide and oxidised low-density
lipoprotein. Food Chemistry 151: 175181.
10. Escudero A, Petzold G, Moreno J, Gonzlez M, Junod J, Aguayo C, Acurio J, Escudero C.
(2013). Supplementation with apple enriched with L-arginine may improve metabolic control and
survival rate in alloxan-induced diabetic rats. Biofactors. 39(5):564-574.
11. Escudero C, Bertoglia P, Hernadez M, Celis C, Gonzlez M, Aguayo C, Acurio J. (2013).
Impaired A2A adenosine receptor/nitric oxide/VEGF signaling pathway in fetal endothelium
during late- and early-onset preeclampsia. Purinergic Signal. 9(2):215-226.
12. Torres S, Campos V, Len C, Rodrguez-Llamazares S, Rojas S, Gonzlez M, Smith C, Mondaca
M. (2012). Biosynthesis of selenium nanoparticles by Pantoeaagglomerans and their antioxidant
activity. J Nanopart Res 14:1236.
13. Daz-Prez F, Radojkovic C, Aguilera V, Veas C, Gonzlez M, Lamperti L, Escudero C, Aguayo
C. (2012). L-arginine transport and nitric oxide synthesis in human endothelial progenitor cells. J
Cardiovasc Pharmacol 60 (5):439-449.
14. Guzmn-Gutirrez E, Westermeier F, Salomn C, Gonzlez M, Pardo F, Leiva A, Sobrevia L.
(2012). Insulin-increased L-arginine transport requires A(2A) adenosine receptors activation in
human umbilical vein endothelium. PLoS One 7 (7): e41705.
15. Westermeier F, Salomn C, Gonzlez M, Puebla C, Guzmn-Gutirrez E, Cifuentes F, Leiva A,
Casanello P, Sobrevia L. (2011). Insulin Restores Gestational Diabetes Mellitus-Reduced
Adenosine Transport Involving Differential Expression of Insulin Receptor Isoforms in Human
Umbilical Vein Endothelium. Diabetes 60 (6):1677-1687.
16. Veas CJ, Aguilera VC, Muoz IJ, Gallardo VI, Miguel PL, Gonzlez M, Lamperti LI, Escudero
CA, Aguayo CR. (2011). Fetal endothelium dysfunction is associated with circulating maternal
levels of sE-selectin, sVCAM1, and sFlt-1 during pre-eclampsia. The Journal of Maternal-fetal &
Neonatal Medicine. 24 (11): 1371-1377.
17. Gonzlez M, Gallardo V, Rodrguez N, Salomn C, Westermeier F, Guzmn-Gutirrez E,
Abarza F, Leiva A, Casanello P, Sobrevia L. (2011). Insulin-stimulated L-arginine transport
requires SLC7A1 gene expression and is associated with human umbilical vein relaxation.
Journal of Cellular Physiology 226 (11): 2916-2924.
18. Sobreva L, Gonzlez M. (2009). A role for insulin on L-arginine transport in fetal endothelial
dysfunction in hyperglycaemia. Current Vascular Pharmacology. 7(4): 467-474.
19. Casanello P, Krause B, Torres E, Gallardo V, Gonzlez M, Prieto C, Escudero C, Faras M,
Sobrevia L. (2009). Reduced L-arginine transport and nitric oxide synthesis in human umbilical
vein endothelial cells from intrauterine growth restriction pregnancies is not further altered by
hypoxia. Placenta. 30(7): 625-633.
20. Puebla C, Faras M, Gonzlez M, Vecchiola A, Aguayo C, Krause B, Pastor-Anglada M,
Casanello P, Sobrevia L. (2008). High D-glucose reduces SLC29A1 promoter activity and
adenosine transport involving specific protein 1 in human umbilical vein endothelium. Journal
Cell Physiology, 215(3):645-656.
21. Casanello P, Torres A, Sanhueza F, Gonzlez M, Faras M, Gallardo V, Pastor-Anglada M, San
Martn R, Sobrevia L. (2005). Equilibrative nucleoside transporter 1 expression is downregulated
by hypoxia in human umbilical vein endothelium. Circulation Research, 97(1):16-24
22. Aguayo C, Casado J, Gonzlez M, Pearson JD, San Martn R, Casanello P, Pastor-Anglada M,
Sobrevia L. (2005). Equilibrative nucleoside transporter 2 is expressed in human umbilical vein
endothelium, but is not involved in the inhibition of adenosine transport induced by
hyperglycaemia. Placenta, 26(8-9):641-653.
23. Vsquez G, Sanhueza F, Vsquez R, Gonzlez M, San Martn R, Casanello P, Sobrevia L. (2004).
Role of adenosine transport in gestational diabetes-induced L-arginine transport and nitric oxide
synthesis in human umbilical vein endothelium. Journal of Physiology, 560:111-122.
24. Gonzlez M, Flores C, Pearson JD, Casanello P, Sobrevia L. (2004). Cell signalling-mediating
insulin increase of mRNA expression for cationic amino acid transporters 1 and 2, and membrane
hyperpolarization in human umbilical vein endothelium. Pflgers Archives - European Journal of
Physiology: 448: 383-394.
Book chapters

1. Gonzlez M. Regulation of expression and activity of L-arginine transporters by nutrients and


hormones: A focus in transcriptional mechanisms regulated by glucose and insulin. In Arginine
in Clinical Nutrition. Eds. Patel V, Preedy V, Rajendram R. Ed. Springer, Switzerland. DOI:
10.1007/978-3-319-26009-9.
2. Escudero C, Gonzlez M, Acurio J, Valenzuela F, Sobrevia L. (2013). The role of placenta in
the fetal programming associated to gestational diabetes. In: Gestational Diabetes - Causes,
Diagnosis and Treatment. Eds. Sobrevia L. Ed. InTech Editorial, Croatia. Chapter 7, pp 135-
162.
3. Sobrevia L, Guzmn-Gutirrez E, Westermeier F, Salomn C, Arroyo P, Palacios E, Bugueo
K, Santos M, Diez de Medina C, Gonzlez M, Escudero C, Salsoso R, Mate A, Vsquez CM,
Pardo F, Leiva A. (2012). Fetoplacental vascular pathophysiology in preeclampsia. In: Recent
Research Developments in Physiology. Eds. SG Pandalai. Ed. Research Signpost, India. Vol. 5,
Chapter 7, pp 105-158.
4. Gonzlez M, Muoz E, Puebla C, Guzmn-Gutirrez E, Cifuentes F, Nien JK, Abarza F,
Leiva A, Casanello P, Sobrevia L. (2011). Maternal and fetal metabolic dysfunction in
pregnancy diseases associated with vascular oxidative and nitrative stress. In: The Molecular
Basis for Origin of Fetal Congenital Abnormalities and Maternal Health: An overview of
Association with Oxidative Stress. Eds. BM Matata, M Elahi. Ed. Bentham, USA. Chapter 8,
pp 98-115.

Manuscript(s) under review


1. Ortiz T, Mosso C, Escudero C, Gonzlez M. Pathophysiological mechanisms of preeclampsia and
supplementation with L-arginine as a therapeutic option. Frontiers in Physiology.

Abstracts (with editorial committee and published in ISI journals from 2010)
1. Saavedra A, Rojas S, Gallegos D, Valdivia L, Cid M, Gonzlez M, Faras M. (2015). Role of
endoplasmic reticulum stress in placental vascular dysfunction associated to insulin resistance in
maternal obesity. Placenta, 36; 4: 501-501.
2. Valdivia L, Rojas S, Saavedra A, Gallegos D, Cid M, Faras M, Gonzlez M. (2015). Differential
expression of CHOP and GADD34 in human fetal endothelium from gestational diabetes. Placenta,
36; 4: 504-504.
3. Quezada S, Saavedra A, Valdivia L, Cid M, Gonzlez M. (2015). Differential expression of
catalytic subunits of NADPH oxidase in human placenta from gestational diabetes. Placenta, 36; 4:
505-505.
4. Valenzuela C, vila P, Rojas S, Villalobos R, Palma C, Gallardo V, Sobrevia L, Gonzlez M.
(2013). Effects of acute and long-term incubation with high D-glucose on mechanisms that induces
nitric oxide and reactive oxygen species in human fetal endothelium. Proc 37th IUPS, PCD349.
5. Cabrera L, Rojas S, Gallardo V, Sobrevia L, Wareing M, Gonzlez M. (2012). A role for large
conductance calcium activated potassium channels (BKCa channels) in L-arginine transport and
vasorelaxation induced by insulin in human feto-placental unit. Proc Physiol Soc 27, PC347.
6. Villalobos R, Careaga P, Cabrera L, Palma C, Rojas S, Gallardo V, Gonzlez M. (2012). High D-
glucose increases the NADPH oxidase 2 and 4 mRNA levels and synthesis of reactive oxygen
species involving the activity of PKC and p38MAPK in HUVEC. Proc Physiol Soc 27, PC181.
7. Careaga P, Villalobos R, Palma C, Rojas S, Gallardo V, Gonzlez M. (2012). Oxidative stress
decreases hCAT-1 protein abundance and L-arginine transport in human fetal endothelium. Proc
Physiol Soc 27, C30.
8. Rojas S, Cabrera L, Careaga P, Palma C, Villalobos R, Gallardo V, Sobrevia L, Gonzlez M.
(2011). Insulin reverses the H2O2-induced vasoconstriction in human feto-placental
microcirculation. Proc Physiol Soc 23, PC240.
9. Cabrera L, vila P, Palma C, Nuez M, Gallardo V, Aguayo C, Sobrevia L, Gonzlez M. (2011).
Role of tyrosine kinases activity in vasodilator mechanisms induced by insulin in human fetal vein.
Proc Physiol Soc 23, PC242.
10. Rodrguez N, vila P, Gallardo V, Sobrevia L, Gonzlez M. (2010). Insulin induces hCATs-
dependent relaxation in human umbilical vein. Proc Physiol Soc 19, PC191.
11. vila P, Rojas S, Gallardo V, Sobrevia L, Gonzlez M. (2010). Chronic incubation with high
extracelular D-glucose increase the contractility of human umbilical vein rings. Placenta 31, A-72.
12. Veas C, Gallardo V, Gonzlez M, Lamperti L, Escudero C, Aguayo C. (2010). Umbilical vein
endothelium activation is associated with high maternal levels of E-selectin, VCAM-1 and SFLT-1
in severe pre-eclamptic pregnancies. Placenta 31 A-47.

Research Grant(s) (current)


VRID-Asociativo 217.033.110-1.0 (PI) (2017-2018). Estudio de las alteraciones elctricas y
mecnicas de membrana plasmtica de clulas endoteliales de vena umbilical humana en
respuesta a la diabetes gestacional o condiciones estresantes relacionadas con la hiperglicemia.
VRID-Enlace 216.033.108-1.0 (PI) (2015-2016) Caracterizacin de la expresin y actividad de
hCATs, NADPH oxidasa y KCa en vasos sanguneos de placenta humana proveniente de
mujeres con diabetes gestacional.
VRID-Asociativo 213.A84.014-1.0 (CoI) (2014-2016). Correlacin entre caractersticas clnicas,
resultados perinatales y marcadores moleculares de disfuncin endotelial en pacientes con
Diabetes Gestacional (w/M Cid, UdeC).
FONDECYT 1120148 (CoI) (2012-2016). Evaluation of novel technologies for the extraction,
purification and stabilization of natural proanthocyanidins: functionalized as possible
phytodrug. (w/ K Fernndez, UdeC).

Previuos grants:
FONDEFIDeA CA12I10374 (CoI) (Chile, 2012-2014). Bio-obtencin de nanopartculas de selenio
encapsuladas en polisacridos para su potencial uso como suplemento alimentario. (w/ M
Mondaca, UdeC).
FONDECYT 11100192 (PI) (2010-2013). Regulation of expression and activity of hCAT-1 by
reactive oxygen species in human endothelium exposed to insulin and high concentrations of
D-glucose.
DIUC 210.033.103-1.0 (PI) (2010/2012). hCATs and NADPH oxidase roles in vascular dysfunction
induced by high extracelular D-glucose in human umbilical vein.
DIUC 211.032.015-1.0 (CoI) (2010/2012). Participacin de Agmatina como Neuromodulador en el
Proceso de Tolerancia y Dependencia de Opiaceos.
CONICYT AT-23070213 (PI) (2007-2009). Transcriptional regulation of SLC7A1by insulin in
human fetal endothelial cells exposed to high extracelular D-glucose: Roles of NF-B, Sp1 and
ROS.

Fields of Specialisation
Vascular function, vascular reactivity, endothelial physiology, membrane transport, diseases of
pregnancy

Research lines
1. Role L-arginine transporters on vascular function
2. Role of insulin as modulator of cardiovascular system
3. Oxidative stress and vascular dysfunction
4. Role of potassium channels in placental vasculature

Contact

Marcelo Gonzlez
Laboratorio de Fisiologa Vascular (LFV)
Department of Physiology
Faculty of Biological Sciences
Universidad de Concepcin
Barrio Universitario s/n, PO Box 160-C, Concepcin 4070386, CHILE.

Tel +56 (2) 2661207 (Direct)


Mobile: +56 (9) 85263058 (Personal)
E-mail: mgonzalezo@udec.cl, mgonzalez79@gmail.com
URL: http://labfisiovascular.blogspot.com/

Web page

http://labfisiovascular.blogspot.cl/

References

Professor Luis Sobrevia


Pontificia Universidad Catlica de Chile
E-mail: sobrevia@med.puc.cl

Dr. Mark Wareing


University of Manchester
E-mail: mark.wareing@manchester.ac.uk