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Name : Sheila Septriyanti Rangkuti

NIM : 4133341016
Class : Biologi Ekstensi B 2013

REVIEW JURNAL

Judul STERILIZATION OF PINEAPPLE EXPLANT FROM
SIPAHUTAR, NORTH SUMATERA, INDONESIA
(ANANAS COMOSUS L.) AND IN VITRO GROWTH
INDUCTION
Jurnal Asian Jr. Of Microbiol. Biotech.Env.Sc
Volume & 17 & 2
Number
ISSN 0972 - 3005
Page 10
Year 2015
Authors 1. Fauziah Harahap
2. Roedhy Poerwanto
3. Sobir
4. Hasruddin
5. Cicik Suriani
6. J. Siallagan
7. Rohyana
Reviewer Sheila Septriyanti Rangkuti
Date 18 Maret 2017

Introduction & Sipahutar pineapple comes from Sipahutar, North Tapanuli,
Aim North Sumatra, Indonesia has the advantage from another
pineapple is taste sweeter, low water content, denser texture,
yellow color, and liked by the community, but production is
very limited and endangered. The authors explain in the
journal that one of the success of tissue culture was a way of
sterilizing the explants before planting. Initiation of the
culture that was free of contaminants is a very important step
because according George et al., (2007) they are started from
small explants and must be grown on nutritive media that are

cleaning the explants. the explants were washed with sterile distilled water. 0. plant tissue cultures must usually be established and maintained in aseptic conditions. Most kinds of microbial organism. detergent. 0.05% NaCIO. Explants were cultured on MS medium+ 5 mg L·1 Kinetin + 0. then peel the outside of explants and put on filter paper to . Material & Materials used in this research is pineapple crown from Method Sipahutar. 2. continued using different sterilants for different durations. and IAA. sterilization. then cleaned with water. Between sterilan I and II. alcohol.5. The aim of the research were to obtain optimum sterilization techniques for pineapple explants (Ananas comosus L. This research consists of experiment series.008% mankozeb. BAP. 4. Growth induction continued with four concentration of Benzyl Amino Purine (BAP) (0. Result The results of this research the authors showed treatment by soaking the explants with a 0. (2006) other sterilization methods may also with keeping it in an autoclave and maintaining the temperature of 121°C for 15 minutes. also favourable for the growth of microorganisms. NaClO. used 5% detergent.) from Sipahutar. 1) mg L·1 were used as a treatment in Murashige and Skoog (MS) medium. MS medium. 2) growth induction of in vitro shoots with Benzyl Amino Purine (BAP) and Indole Acetate Acid (IAA) plant growth regulators (PGR).002% streptomycin sulphate for 1 hour followed by 1. At the book written by Edwin et al. 6) mg L-1 and three concentration of IAA (0. compete adversely with plant material growing in vitro.5 mgL· 1 IAA for induction of early growth. In the research the tools used is standard tissue culture.. and in particular bacteria and fungi. and in vitro propagation. sterile distilled water. Sterilization techniques used were of 9 kinds. growth induction.

83% of Moris MGB explants formed calli on 85.93 pM levels of auxin NAA caused calli induction in Moris while levels 32. 53. it means that the use of pure NaCl without the combination can produce high explant .71. produced high aseptic culture (55. A mean total value of 108. 53.19 pM also induced calli Josapine.4-D in Murashige and Skoog solid media.2%) then giving of BAP and IAA significantly affect to appearance of shoots. was investigated. 2.4-D auxin treatments failed to induce calli in both cultivars.5 shoots were regenerated within four weeks from 20 original shoots cultured on medium containing 1 mg/L BAP.22. However. and then planted. under six concentration levels of auxin NAA and six concentration levels of 2.71 pM NAA.71 and 75.19 and 85. The percentage of MGB calli formation increased with increasing time of culturing. At 6 weeks of culturing. according research Silva et al.93 pM NAA. In this journal said that the best treatment was a combination of MS + 2 mg L-1 BAP + 0 mg L-1 IAA. while 50% of Josapine MGB explants formed calli on 53.dry.. but according another journals written by Ibrahim (2013) use MS medium mixed with 2% NaCl gave low significantly percentage of survival explants reached 15% but MS medium without NaCl treatment of shoot multiplication stage gave high significantly percentage of survival explants reached 85%.6%) and showed the highest growth (22. But.. 75. (2008) the induction of callus pineapple from Meristemic Globular Bodies (MGB). number of leaves except shoots hight this is supported by research of Al-Saif et al. Adjei (2001) says in his research result that the concentration of BAP in the culture medium significantly affected morphogenesis. (2011) that BAP at the concentration of 2 mg/l was effective for pinapple shoot growth and development. number of shoots.

Sankar. Abstract able to describe clearly the aim.. Effect of Benzylaminopurine (BAP) in Shoot Regeneration in Pineapple (Ananas Comosus L. Language used by the author easily understood by the reader. Botany. Sekar. R. 2008. 5. 2005. Edwin. 2. and Jan. Analysis is very detailed and easy to understand. F. Journal of The Kwarne Nkrumah University of Science and Technology 21 (1-3) : 33. M. George. Farahani. P.Hall. S....D. Effects Of Benzylaminopurine and Naphthalene Acetic Acid on Proliferation And Shoot Growth of Pineapple (Ananas Comosus L. The Netherlands. 4. 2014. Plant Archives 14 (1) : 337-341. Plant Propagation by Tissue Culture 3rd Edition.35. The explanation of the research method in this journal is very clear as it is equipped with a table describing the material used in each treatment and the duration. The number of words in the abstract exceed 250 words 2. Springer. Tamil Nadu Textbook Coorporation.Y.. and the results obtained. this is different than research journals written by Farahani (2014). Merr) In Iran. the methodology. Merr) Cultured In Vitro. Al-Saif. African Journal of Biotechnology 10 (27) :5291-5295. 3. and Munusamy. Weakness The weakness of this journal is : 1. Higher Secondary Second Year. P. Advantages The advantages of this journal is : 1. . The Journal does not provide recommendations for future research Daftar Pustaka Adjei. G.K. 2001. Systematics of writing has been structured and clear. Merr) In Vitro. Micropropagation and Growth Of In Vitro Pineapple (Ananas Comosus L. T. growth.E. A. 2011. College Road Chennai.

International Journal of Farming and Allied Sciences 2 (9) : 206-210.) cv.Ibrahim. Kadir.) cv. 2008. S.. and Kadzimin.. M. M.A. Majid A. Callus Induction in Pineapple (Ananas Comosus L. 2013. . Moris and Josapine. De. Effect of NaCl Stress on Pineapple Plant (Ananas comosus Merr. (L.A. International Journal of Agricultural Research 3 (4) : 261-267. Aziz..E. Silva. A. Del Monte) In Vitro.