Name : Ayu Puspita Budiputri

NIM : 4133341012
Class : Biology Eks.B.2013

Identity of journal
Name of journal : Sterilization of Pineapple Explant from Sipahutar, North
Sumatera, Indonesia (Ananas comosus L.) And In Vitro
Growth Induction.
Publisher : Asian Journal of Microbiology, Biotechnology And
Enviromental Science Paper.
Issue :2
Year : 2015
Volume : 21
Authors : 1. Fauziah Harahap
2. Roedhy Poerwanto
3. Sobir
4. Hasruddin
5. Cicik Suriani
6. J. Siallagan
7. Rohyana
Date of receipt : 20 October 2015
Introduction and Aims of Research
Reasons such study was done because the Sipahutar pineapple have the
advantage that it tastes more sweet, water is low content, denser texture, yellow
color and liked by the community, but production is very limited and endangered.
The aims of the research were to obtain optimum sterilization techniques
for pineapple explants (Ananas comosus L.) from Sipahutar and growth induction
of in vitro shoots with Benzyl Amino Purine (BAP) and Indole Acetate Acid
(IAA) plant growth regulators (PGR).
Pineapple is one of the major economically important fruit crop in tropical
zone. Comparing its annual world production which exceeds 15 billion kg per

2005). the explants were washed with sterile distilled water. then cleaned with water. NaClO. the plants can be reproduced at anytime as needed for propagation factor high. So.. sterilization is the technique employed to get rid of the microbes such as bacteria and fungi in the culture medium and plant tissues.3 billion kg per year.. 2013). The tools used for this research were standard tissue culture. 0. Between sterilan I and II. In this research noted that famers in Sipahutar develop these plants used crown. 6) mg L-1 and three concentration of IAA (0. detergent. For vegetative propagation of the plants. continued using different sterilants for different durations. But the seeds of these plants are very slow to germinate and therefore are not used for commercial purposes. The culture medium can be sterilised by keeping it in an autoclave and maintaining the temperature of . used 5% detergent. Through tissue culture. Growth induction continued with four concentration of Benzyl Amino Purine (BAP) (0. one alternative to solve this problem was by tissue culture techniques. Explants were cultured on MS medium+ 5 mg L· 1 Kinetin + 0. BAP and IAA. India produces nearly 1. 2. alcohol.year. MS medium. 1) mg L·1 were used as a treatment in Murashige and Skoog (MS) medium. In case of industrial scale production suckers and heaps are also used (Dutta et al. While. Of some related research. Sterilization techniques to cleaning the explants. sterile distilled water.5. So it can produce a uniform and quality seeds guaranteed. This technology has been widely used for get the uniform seedlings especially on horticulture crops. 4.5 mgL· 1 IAA for induction of early growth. divide old plants as seed of a comparatively very limited for large land fill. uniform and reasonable prices by farmes is the first step to increase the production of Sipahutar pineapple (Amin et al. The availability of quality seeds. it is important to sterilize the culture medium and plant tissue. crowns and slips have been successfully used over years. buds. Materials and Methods Materials used in this research is pineapple crown in Sipahutar.

Various chemical compounds..05% NaCIO. This is due to the exposure of plants to many new ex vitro situations such as low humidity.. sterilization of explants and growth induction with nine sterilization procedure was performed on Sipahutar pineapple crown.121°C for 15 minutes. treating the plant material with disinfecting chemicals to kill superficial microbes. antibiotics showed an influence on the time of contaminated.002% streptomycin sulphate for 1 hour followed by 1. produced high aseptic culture (55. 2013). Therefore. the percentage of aseptic culture. high level of irradiation.008% mankozeb. The threats for survival in ex vitro can be overcome by acclimatiz. uniform and mass production of pineapple plantlets. 0. plants under in vitro condition need to be acclimatized by different options.nection. 2008). In vitro propagation is a crucial technique for disease free. 2005). In this research.6%) and showed the highest growth . and in particular bacteria and fungi. compete adversely with plant material growing in the plants with gradual lowering air humidity tem. and sterilising the tools used for dissection and the vessels and media in which cultures are grown (George et al. as far as possible. air flow and irradiation level (Mengesha et al. then peel the outside of explants and put on filter paper to dry.perature. Most kinds of microbial organism. explants were necrotic.draulic conductivity of the roots and low root-stem con. Therefore. performance of the explants and growth of explant. Nevertheless. High loss or damage of in vitro raised plants can occurred when transferred to ex vitro conditions because of the transfer shock. rapid. water deficit because of the poor hy. Results and Discussion Plant tissue cultures must usually be established and maintained in aseptic conditions. and then planted.. ultimate success of in vitro produced plantlets depends upon the successful transfer and establishment of plants in ex vitro conditions. The plant tissue (inoculum) is to be surface sterilised (Edwin et al. explants must be free from microbial contaminants when they are first placed on a nutrient medium. This usually involves growing stock plants in ways that will minimise infection. The results of this research showed treatment by soaking the explants with a 0.

Advantages and Weaknees To the advantages of this journal. IAA had no effect on shoot height. Cytokinin can increase cell division. to one leaf) causes the treated organ to become an active sink for amino acids. auxin must be present.2%). They particularly stimulate protein synthesis and participate in cell cycle control. 2015). often together with auxins. and giving of BAP and IAA significantly affect to appearance of shoots. The effect of cytokinins is most noticeable in tissue cultures where they are used. number of leaves except shoots hight. Cytokinins comprise a separate class of growth substances and growth regulators.g. there are some things that become critical review among other things the author did not write clearly about the specifications of materials used in parts of materials in research journals should also be the tools used in the study included to more clearly. but for the elongation of cells required addition auxin.(22.. which then migrate to the organ from surrounding sites. However. on the whole journal is quite complete and meet the standard of writing. . to stimulate cell division and control morphogenesis (Mohajer et al. number of shoots. Cytokinin application to a single site in the plant (e. In this research. They produce various effects when applied to intact plants. It is perhaps for this reason that they can promote the maturation of chloroplasts and delay the senescence of detached leaves. The possibility needed combination of a certain concentration between BAP and IAA to induce and increase research shoot height of pineapple from Sipahutar.

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