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BC-5150/BC-5000

AUTO HEMATOLOGY ANALYZER

SERVICE MANUAL
Introduction
Before starting to service this instrument, please read this manual thoroughly for proper
service.
After reading this manual, make sure it is well kept so that it is available when needed.

Product Name: Auto Hematology Analyzer


Model: BC-5150/BC5-5000
Registered Address: F1-4, Mindray Building, Keji 12th Road South, Hi-tech Industrial Park,
Nanshan, Shenzhen
Production Address: Building A3, Honghualing Industrial Park, Liuxian Road, Nanshan,
Shenzhen

Intellectual Property Statement


SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD. (hereinafter called Mindray)
owns the intellectual property rights to this manual and the relevant product.

2012 Shenzhen Mindray Bio-medical Electronics Co., Ltd. All rights Reserved.

Release, amendment, reproduction, distribution, rent, adaption and translation of this


manual in any manner whatsoever without the written permission of Mindray is strictly
forbidden.

, , , are registered trademarks or trademarks


owned by Mindray.

Microsoft Corporation owns the copyright of SQL SERVER 2005 EXPRESS EDITION. 2005
Microsoft Corporation. All rights reserved.

Statement
Mindray is responsible for safety, reliability and performance of this product only in the
condition that:
all installation operations, expansions, changes, modifications and repairs of this
product are conducted by Mindray authorized personnel;

all replacement parts and supporting accessories and consumables involved in the
service are original Mindray parts or Mindray authorized parts;


the relevant electrical installation complies with the applicable national requirements;

the product is operated in accordance with this service manual.

Repair Service
Free Service:
Free service is provided for any product within the scope specified by Mindray warranty
rules.
Paid Service:
Mindray offers paid service for any product beyond the scope specified by Mindray
warranty rules;

even during the warranty period, only paid service is available if the need for service is caused
by the following reasons: artificial damage; improper use; grid voltage beyond the specified
range of the device; irresistible natural disasters; replacement with parts and consumables not
authorized by Mindray or service work by personnel not authorized by Mindray.

Return Procedure
In the event that it becomes necessary to return this product or part of this product to
Mindray, the following procedure should be followed::
Obtain return authorization: Contact the Mindray Service Department and obtain a
Customer Service Authorization (Mindray) number. The Mindray number must appear
on the outside of the shipping container. Returned shipments will not be accepted if the
Mindray number is not clearly visible. Please provide the model number, serial number,
and a brief description of the reason for return.

Freight policy: The customer is responsible for freight charges when shipping the
product to Mindray for service (including customs charges).

Company Contact
Manufacturer:: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.

Address: Mindray Building, Keji 12th Road South, Hi-tech Industrial Park, Nanshan, Shenzhen

Postal Code: 518057

Website: www.mindray.com

24-hour Service Hotline: 4007005652

Phone: +86 755 81888998


Fax: +86 755 26582680

II
This system is only intended for use by qualified service personnel trained by
Mindray or Mindray's agents.
It is important for the hospital or organization that employs this equipment to
carry out a reasonable service/maintenance plan. Neglect of this may result in
machine breakdown or injury of human health.
Be sure to operate the analyzer under the situation specified in this manual;
otherwise, the analyzer will not work normally and the analysis results will be
unreliable, which would damage the analyzer components and cause personal injury.

This manual is only provided for qualified service personnel trained by Mindray or
Mindray's agents.

III
Table of Contents

1 Overview .................................................................................................................... 1-1


1.1 Overview ..................................................................................................................... 1-1
1.2 Who Should Read This Manual .................................................................................. 1-1
1.3 How to Find Information ............................................................................................ 1-1
1.4 Conventions Used in This Manual .............................................................................. 1-2
1.5 Special Terms Used in This Manual............................................................................ 1-2
1.6 Symbol ........................................................................................................................ 1-4

2 Specifications ............................................................................................................. 2-1


2.1. Product Name .............................................................................................................. 2-1
2.1.1 Physical Specifications ....................................................................................... 2-2
2.1.2 Electrical Specifications ..................................................................................... 2-2
2.1.3 Environment Requirements ................................................................................ 2-3
2.1.4 Specifications ..................................................................................................... 2-3
2.2. Testing Parameters ...................................................................................................... 2-3
2.3. Performance Requirements ......................................................................................... 2-5
2.3.1 Background/Blank Count ................................................................................... 2-5
2.3.2 Carryover ........................................................................................................... 2-5
2.3.3 Repeatability ...................................................................................................... 2-6
2.3.4 Linearity Range .................................................................................................. 2-7
2.4. Display Range ............................................................................................................. 2-8
2.5. Product Description..................................................................................................... 2-8
Main unit ................................................................................................................... 2-12
Power/Status indicator............................................................................................... 2-12
Power switch ............................................................................................................. 2-12
[Aspirate] key ............................................................................................................ 2-13
USB port ................................................................................................................... 2-13
2.6. Product Configuration ............................................................................................... 2-13
2.7. Reagents, Controls and Calibrators ........................................................................... 2-13
2.7.1 Reagents ........................................................................................................... 2-14
2.7.2Reagent Consumption Volume .................................................................................... 2-14
2.7.3 Controls and Calibrators................................................................................... 2-15
2.8. Information Storage Capacity ................................................................................... 2-15

3 Operation Principles ................................................................................................. 3-1


3.1 Overview ..................................................................................................................... 3-1
3.2 Workflow..................................................................................................................... 3-1
3.3 Sample aspiration ........................................................................................................ 3-2
3.4 White Blood Cell Measurement .................................................................................. 3-2
Laser flow cytometry .................................................................................................. 3-2

1
Table of Contents

3.5 Hemoglobin Concentration Measurement................................................................... 3-3


Colorimetric method ................................................................................................... 3-3
Hemoglobin concentration parameters ........................................................................ 3-3
Impedance method ...................................................................................................... 3-3
Time Measurement ...................................................................................................... 3-4
Red blood cell parameters ........................................................................................... 3-4
Platelet parameters ...................................................................................................... 3-5
3.6 Parameter Flags ............................................................................................................... 3-6

4 Software and Interface.............................................................................................. 4-1


4.1. Start-up ........................................................................................................................ 4-1
Version Check ............................................................................................................. 4-1
4.2. Login ........................................................................................................................... 4-2
4.2.1 Log in using service level username and password............................................ 4-2
4.2.2 Login Check ....................................................................................................... 4-2
4.3. Review ........................................................................................................................ 4-4
4.3.1 Stability Trend Graph ......................................................................................... 4-4
4.3.2 Trend Graph ....................................................................................................... 4-5
4.4. Calibration ................................................................................................................... 4-5
4.4.1 Calibration Factor and Transfer Factor .............................................................. 4-5
4.4.2 Calibration .......................................................................................................... 4-7
4.5. Sample Probe Debug ................................................................................................... 4-8
4.6. Temperature Calibration.............................................................................................. 4-8
4.7. Gain Calibration .......................................................................................................... 4-9
4.8. Gain Setting................................................................................................................4-11
4.9. Performance .............................................................................................................. 4-12
4.9.1 Background test................................................................................................ 4-12
4.9.2 Reproducibility................................................................................................. 4-13
4.9.3 Carryover ......................................................................................................... 4-14
4.9.4 Reference range of normal samples ................................................................. 4-14
4.10. Advance Toolbox ...................................................................................................... 4-15
4.10.1 System Configuration..................................................................................... 4-15
4.10.2 One-key Export .............................................................................................. 4-16
4.11. Software Update ........................................................................................................ 4-17
4.12. Status Indicator.......................................................................................................... 4-18
4.13. Buzzer ....................................................................................................................... 4-18

5 Data Transmission ..................................................................................................... 5-1


5.1. LIS Connection ........................................................................................................... 5-1
5.2. Data Management Software Setup .............................................................................. 5-3
5.3. Communication Error Analysis ................................................................................... 5-4
5.3.1 Physical Connection ........................................................................................... 5-4
5.3.2 Communication Setup ........................................................................................ 5-4
5.3.3 Network Firewall ............................................................................................... 5-4

2
Table of Contents

6 Optical System ........................................................................................................... 6-1


6.1. Introduction to the Principles of Optical System ........................................................ 6-1
6.1.1 Operation Principles ........................................................................................... 6-1
6.1.2 Beam Path of the Optical system ....................................................................... 6-2
6.2. Physical Structure........................................................................................................ 6-2
6.2.1 Overall Structure ................................................................................................ 6-2
6.2.2 Light Source Assembly ...................................................................................... 6-4
6.2.3 Flow Cell Assembly ........................................................................................... 6-4
6.2.4 Optical Substrate Assembly ............................................................................... 6-5
6.2.5 Preamplifier and Shield Shell............................................................................. 6-5
6.2.6 Shield Shell Assembly........................................................................................ 6-6
6.2.7 Scattering Detection Assembly .......................................................................... 6-6
6.2.8 Laser Control Board ........................................................................................... 6-7
6.3. Determinating the Optical System Status .................................................................... 6-7
6.4. Maintenance and Replacement of the Optical System ................................................ 6-9
6.4.1 Maintenance of the Optical System.................................................................... 6-9
6.4.2 Replacement of the Optical System ................................................................. 6-14

7 Fluidics ....................................................................................................................... 7-1


7.1. Measurement Flow ...................................................................................................... 7-1
7.1.1 WBC&HGB Channel ......................................................................................... 7-2
7.1.2 RBC/PLT Channel.............................................................................................. 7-4
7.2. Sample Volume ........................................................................................................... 7-4
7.3. Temperature of Fluidics .............................................................................................. 7-5
7.4. Reagent Consumption Volume .................................................................................... 7-5
7.5. Introduction to Fluidic Parts........................................................................................ 7-6
7.5.1 Mindray Valves .................................................................................................. 7-6
7.5.2 2-way Mindray Pressure-proof Valve ................................................................. 7-6
7.5.3 LVM Fluidic Valve ............................................................................................. 7-7
7.5.4 Pinch Valve......................................................................................................... 7-7
7.5.5 Liquid Filter ....................................................................................................... 7-8
7.5.6 Syringe Linkage ................................................................................................. 7-9
7.5.7 Electromagnetic Metering Pump ........................................................................ 7-9
7.5.8 preheat bath ...................................................................................................... 7-10
7.5.9 Vacuum Pump ...................................................................................................7-11
7.5.10 Sample Probe ................................................................................................. 7-12
7.5.11 Probe Wipes.................................................................................................... 7-12
7.5.12 Hydraulic pressure sensor .............................................................................. 7-13
7.5.13 1-way Valve .................................................................................................... 7-14
7.5.14 Baths............................................................................................................... 7-14
7.6. Detailed Introduction of Fluidic Structure ................................................................ 7-14
7.6.1 Sampling and dispensing channel .................................................................... 7-15
7.6.2 WBC&HGB Channel ....................................................................................... 7-15
7.6.3 RBC/PLT Channel............................................................................................ 7-17

3
Table of Contents

7.6.4 Precautions for Assembly and Service ............................................................. 7-18


7.7. Introduction to Sequences ......................................................................................... 7-19
7.7.1 Measurement sequence in WB-CBC+DIFF mode ........................................... 7-19
7.7.2 Measurement sequence in PD-CBC+DIFF mode ............................................ 7-32
7.7.3 Measurement sequence in CBC mode.............................................................. 7-33
7.7.4 Introduction to the Maintenance Sequences ..................................................... 7-33

8 Hardware System ...................................................................................................... 8-1


8.1. Hardware System Overview........................................................................................ 8-1
8.1.1 Functional Block Diagram ................................................................................. 8-1
8.1.2 Electrical Connection Block Diagram................................................................ 8-2
8.1.3 System Troubleshooting ..................................................................................... 8-2
8.2. Digital Control Board.................................................................................................. 8-4
8.2.1 Overview ............................................................................................................ 8-4
8.2.2 Components........................................................................................................ 8-4
8.2.3 Adjustment and Tests ......................................................................................... 8-8
8.2.4 Troubleshooting.................................................................................................8-11
8.3. Analog Drive Board .................................................................................................. 8-15
8.3.1 Overview .......................................................................................................... 8-15
8.3.2 Components...................................................................................................... 8-15
8.3.3 Sockets and Indicators...................................................................................... 8-19
8.3.4 Troubleshooting................................................................................................ 8-23
8.4. Power Board .............................................................................................................. 8-26
8.4.1 Overview .......................................................................................................... 8-26
8.4.2 Replacement and Connection ........................................................................... 8-27
8.4.3 Troubleshooting................................................................................................ 8-27
8.5. Optical Boards........................................................................................................... 8-28
8.5.1 Optical Path and Optical System Workflow ..................................................... 8-28
8.5.2 Functions of Optical Boards............................................................................. 8-29
8.5.3 Troubleshooting................................................................................................ 8-31
8.6. Touchscreen Drive Board .......................................................................................... 8-33
8.6.1 Overview .......................................................................................................... 8-33
8.6.2 Components...................................................................................................... 8-33
8.6.3 Troubleshooting................................................................................................ 8-33
8.7. Indicator Board ......................................................................................................... 8-34
8.7.1 Overview .......................................................................................................... 8-34
8.7.2 Components...................................................................................................... 8-34
8.7.3 Troubleshooting................................................................................................ 8-35
8.8. Motor, Photocoupler and Microswitch ...................................................................... 8-35
8.8.1 Overview .......................................................................................................... 8-35
8.8.2 Troubleshooting................................................................................................ 8-36
8.9. Liquid sensor board ................................................................................................... 8-37
8.9.1 Overview .......................................................................................................... 8-37
8.9.2 Composition ..................................................................................................... 8-37

4
Table of Contents

8.9.3 Troubleshooting................................................................................................ 8-37

9 Heating System .......................................................................................................... 9-1


9.1. Heating System Overview........................................................................................... 9-1
9.2. Heating System ........................................................................................................... 9-1
9.2.1 Diluent Heating System ..................................................................................... 9-1
9.2.2 Optical heating system ....................................................................................... 9-3

10 Mechanical System .................................................................................................. 10-1


10.1. Mechanical System Overview................................................................................... 10-1
Front view ................................................................................................................. 10-1
Back view.................................................................................................................. 10-2
Left view ................................................................................................................... 10-4
Left view ................................................................................................................... 10-6
10.2. Components .............................................................................................................. 10-6
10.2.1 Introduction .................................................................................................... 10-6
10.2.2 Overall ............................................................................................................ 10-7
10.2.3 Main unit ........................................................................................................ 10-8
10.2.4 Panel Module (BC-5150) ............................................................................. 10-12
10.2.5 Panel Module (BC-5000) ............................................................................. 10-13
10.2.6 Syringe Assembly......................................................................................... 10-14
10.2.7 Sample Probe Assembly ............................................................................... 10-15
10.2.8 RBC count pool module ............................................................................... 10-16
10.2.9 HGB count pool module .............................................................................. 10-17
10.2.10 Pump Assembly.......................................................................................... 10-18
10.2.11 Power Backplane Assembly ....................................................................... 10-18
10.2.12 Optical System ........................................................................................... 10-20
10.2.13 Power Assembly ......................................................................................... 10-21
10.3. Removal and Installation......................................................................................... 10-21
10.3.1 Tools ............................................................................................................. 10-21
10.3.2 Preparation for Disassembly ........................................................................ 10-21
10.4. Disassembling the Main Unit .................................................................................. 10-22
10.4.1 Removing the Back plate ............................................................................. 10-23
10.4.2 Removing the Analog drive board PCBA .................................................... 10-23
10.4.3 Removing the Pinaster board or the SD card ............................................... 10-24
10.4.4 Removing the power backplate Assembly ................................................... 10-25
10.4.5 Removing the Fan Assembly........................................................................ 10-26
10.4.6 Removing the Right Panel............................................................................ 10-26
10.4.7 Removing the RBC count pool module(FRU) ............................................. 10-27
10.4.8 Removing the Ruby Red Cell Counter(D50um) .......................................... 10-28
10.4.9 Removing the HGB amp module ................................................................. 10-29
10.4.10 Removing the Liquid detect board PCBA .................................................. 10-31
10.4.11 Removing the Reagent Bottle plate cosmetic piece ................................... 10-31
10.4.12 Removing the preheat Assembly ................................................................ 10-32

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Table of Contents

10.4.13 Removing the Electromagnet Pinch Valve Assembly ................................ 10-33


10.4.14 Removing the Right Valve Assembly ......................................................... 10-34
10.4.15 Removing the Pump ................................................................................... 10-35
10.4.16 Removing the Vacuum Chamber Assembly ............................................... 10-36
10.4.17 Removing the Top Cover ........................................................................... 10-36
10.4.18Removing the Temperature detection assembly .......................................... 10-37
10.4.19 Removing the Sampling Probe Assembly .................................................. 10-37
10.2.20 Replace the Horizontal Motor of the Sampling Probe Assembly............... 10-38
10.4.21 Replace the Vertical Motor of the Sampling Probe Assembly ................... 10-39
10.4.22 Removing the Probe wipe .......................................................................... 10-39
10.4.23 Replacing the Sample Probe ...................................................................... 10-40
10.4.24 Removing the Syringe Motor Position Sensor ........................................... 10-41
10.4.25 Removing the transducer discreteness ....................................................... 10-42
10.4.26 Removing the Panel Module ...................................................................... 10-43
10.4.27 Removing the Indicator board PCBA ........................................................ 10-43
10.4.28 Removing the 6301 touch control board PCBA ......................................... 10-44
10.4.29 Removing the Touch Panel......................................................................... 10-45
10.4.30 Removing the LCD Module ....................................................................... 10-46
10.4.31 Removing the Microswitch Assembly ....................................................... 10-46
10.4.32 Removing the Syringe ................................................................................ 10-47
10.4.33 Removing the Syringe Motor ..................................................................... 10-48
10.3.34 Removing the Syringe Motor Position Sensor Assembly .......................... 10-49
10.4.35 Removing the liquid detect assembly......................................................... 10-49
10.4.36 Removing the Electromagnetic Pump/Valve from the Front Panel............ 10-50
10.4.37 Removing the Left Door Assembly ............................................................ 10-51
10.4.38 Removing the Optical System .................................................................... 10-52
10.4.39 Removing the Optical System Cover ......................................................... 10-52
10.4.40 Replacing the RBC/WBC Isolation Chamber Filter .................................. 10-52
10.4.41 Replacing the Power Board PCBA ............................................................ 10-53

11 Troubleshooting ........................................................................................................11-1

12 Debug........................................................................................................................ 12-1
12.1 Mechanical Position Adjustment ................................................................................. 12-1
12.2 Commissioning of detection elements ........................................................................ 12-2
12.2.1 Calibrate and verify the preheat temperature ................................................. 12-2
12.2.2 Counting channel measurement .................................................................. 12-3

13 Commissioning and Verification After Servicing ................................................. 13-1

14 Service BOM ............................................................................................................ 14-1

15 Appendices ............................................................................................................... 15-1

6
Table of Contents

A. Fluidic diagram ........................................................................................................ A-1

B. Connection and Tube ................................................................................................ B-1

C. Hardware block diagram......................................................................................... C-1

D. Cables and Wires ...................................................................................................... D-2

E. Appendix Table .......................................................................................................... E-1

7
1 Overview

1.1 Overview
This chapter describes how to use the service manual. In this manual, the repair methods
of BC-5150/BC-5000 are described in detail. Before servicing BC-5150/BC-5000, please carefully
read and understand the content in order to properly carry out equipment maintenance and
ensure the safety of service personnel.
This manual must be used in conjunction with the BC-5150/BC-5000 Operators manual. It
does not contain information and procedures already covered in the Operators manual of
BC-5150/BC-5000.

Be sure to operate and service the analyzer strictly as instructed in this manual and the
operators manual.

1.2 Who Should Read This Manual


To use this manual effectively, you need to have the following capacities:

Comprehensive knowledge of electric circuit and fluidic system;


Comprehensive knowledge of reagents;
Comprehensive knowledge of quality control;
Thorough understanding of troubleshooting;
The ability to operate the analyzer skillfully
The ability to use basic mechanical tools and understand related terminology;
The ability to use a digital voltmeter and an oscilloscope masterly;
And the ability to read pneumatic/hydraulic schematics and understand related
terminology.

1.3 How to Find Information


This manual contains 14 chapters and 4 appendices. Refer to the table below to find the
information you need.

If you want to See


BC-5150/BC-5000's physical specifications Chapter 2 Specifications
BC-5150/BC-5000's parameters, respective ranges and test Chapter 3 Operation Principles
principle
interface introduction and upgrading of Chapter 4 Software and
BC-5150/BC-5000's software Interface

1-1
Overview

BC-5150/BC-5000's external interface settings Chapter 5 Data Transmission


learn about the optical system of BC-5150/BC-5000's and Chapter 6 Optical System
its maintenance methods
composition, dosage, basic channels and time sequence of Chapter 7 Fluidic System
BC-5150/BC-5000's fluidic system
BC-5150/BC-5000's hardware structure; composition, Chapter 8 Hardware System
commissioning and testing point and troubleshooting of
each board
BC-5150/BC-5000's heating principle Chapter 9 Heating System
BC-5150/BC-5000's error and troubleshooting Chapter 10 Troubleshooting
BC-5150/BC-5000's structure, disassembly and Chapter 11 Mechanical System
verification
Debug after BC-5150/BC-5000 being serviced Chapter 12 Debug
Debug and verification after BC-5150/BC-5000 being Chapter 13 Debug and
serviced Verification After Servicing
BC-5150/BC-5000's service BOM Chapter 14 Service BOM
BC-5150/BC-5000's fluidic diagrams Appendix A Fluidic Diagrams
BC-5150/BC-5000's fluidic tube connectors Appendix B Lists of tube
connectors
BC-5150/BC-5000's hardware diagrams Appendix C Hardware
Diagrams
BC-5150/BC-5000's cables and wires Appendix D Cables and Wires

1.4 Conventions Used in This Manual


This manual uses certain typographical conventions to clarify meaning in the text:

Format Meaning
[] all capital letters enclosed in [ ] indicate a key name
(either on the pop-up keyboard or the external keyboard)
letters included in " " indicate text you can find on the
screen of BC-5150/BC-5000
italic letters indicate titles of the chapters that are referred
to

All illustrations in this manual are provided as examples only. They may not necessarily
reflect your analyzer setup or data displayed.

1.5 Special Terms Used in This Manual


You will find the following symbols in this manual.
When you read < It means <

1-2
Overview

read the statement below the symbol. The statement is alerting


you to a potentially biohazardous condition.

read the statement below the symbol. The statement is alerting


WARNING you to an operating hazard that can cause personnel injury.

read the statement below the symbol. The statement is alerting


CAUTION you to a possibility of analyzer damage or unreliable analysis
results.

read the statement below the symbol. The statement is alerting


NOTE you to information that requires your attention.

All the substances (samples, controls, calibrators, reagents and liquid wastes)
and areas in contact with these substances are potentially infectious. Wear proper
personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory
procedures when accessing these substances and areas in the laboratory.
In the event of main unit leak, the leaking fluid is biohazardous.

WARNING
It is important for the hospital or organization that employs this equipment to carry out a
reasonable service/maintenance plan. Neglect of this may result in machine breakdown or
injury of human health.
Never use combustible gas (e.g. anesthetic) or combustible liquid (e.g. ethanol) around the
analyzer. Otherwise, the risk of explosion may exist.
When servicing the analyzer, be sure to turn off the power. Servicing the analyzer when it
is on may bring risk of electric shock or damage to electronic components.
Please connect the analyzer to a socket having sole fuse and protective switch. Do not use
the same fuse and protective switch with other equipment (e.g. life supporting equipment).
Otherwise, the equipment failure, over current or impulse current that occurs at the startup
moment may lead to tripping.
To prevent personal injury during the maintenance, keep your clothes, hairs and hands
from the moving parts, such as sample probe, pincher and piercer.
Possible mechanical movement of the warned position may lead to personal injury during
normal operation, removal and service verification.
Be sure to dispose of reagents, waste, samples, consumables, etc. according to government
regulations.

1-3
Overview

The reagents are irritating to eyes, skin and diaphragm. Wear proper personal protective
equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when
handling them in the laboratory.
If the reagents accidentally spill on your skin, wash them off with plenty of water and if
necessary, go see a doctor; if the reagents accidentally spill into your eyes, wash them off
with plenty of water and immediately go see a doctor.

CAUTION
Improper maintenance may damage the analyzer. Maintain the analyzer strictly as
instructed by the service manual and inspect the analyzer carefully after the maintenance.
For problems not mentioned in the service manual, contact Mindray customer service
department for maintenance advice.
To prevent personal injury or damage to equipment components, remove metal jewelry
before maintaining or servicing electronic components of the equipment.
Electrostatic discharge may damage electronic components. If there is a possibility of ESD
damage with a procedure, then do that procedure at an ESD workstation, or wear an
antistatic wrist strap.

NOTE
The operator is required to follow the instructions below this symbol. The
instructions will emphasize important information or information that requires
particular attention of the operator.

1.6 Symbol
Symbols used in this service manual:

Symbol Meaning
The operator is required to follow the instructions below
this symbol. Failure to do so may place the operator at a
potential risk of biohazard.
The operator is required to follow the instructions below
WARNING this symbol. Failure to do so may cause personal injury.

The operator is required to follow the instructions below


CAUTION this symbol. Failure to do so may cause malfunction or
damage of the product or affect the test results.

1-4
Overview

The operator is required to follow the instructions below


NOTE this symbol. The instructions will emphasize important
information or information that requires particular attention
of the operator.

The analyzer system may contain the following symbols:

CAUTION
Ensure the labels are in good condition and not damaged while servicing the analyzer.

When you see


It means

CAUTION, CONSULT ACCOMPANYING


DOCUMENTS.
Note: It is recommended that the reader
refers to the accompanying documents for
important safety information.
BIOLOGICAL RISK

WARNING, LASER BEAM

PROTECTIVE EARTH (GROUND)

USB port

Network interface

ALTERNATING CURRENT

FOR IN VITRO DIAGNOSTIC USE

Batch code

1-5
Overview

USE BY (YYYY-MM-DD)

Serial number

MEASUREMENT AUTHORIZATION
SYMBOL

DATE OF MANUFACTURE

Pricking danger

Manufacturer

TEMPERATURE LIMITATION

CONSULT INSTRUCTIONS FOR USE

The device fully complies with requirements


of EU IVD Directive 98/79/EC

This electronic product contains certain toxic


substances, and has an Environmental
Protection Use Period (EPUP) of 20 years. It
can be used safely during the EPUP, but
shall be recycled after the EPUP.

1-6
2 Specifications

2.1. Product Name


Name: Auto Hematology Analyzer
Model: BC-5150/BC-5000

BC-5150 Appearance

BC-5000 Appearance

2-1
Specifications

2.1.1 Physical Specifications

Height

Depth

Width

Table 2-1 Dimensions and weight

BC-5150/BC-5000 Overall

Width 325 mm

Height 435 mm (rubber feet


Dimensions
included)

Depth 410 mm

Weight 25Kg

2.1.2 Electrical Specifications

Table 2-2 Main unit power supply

Parameter Value
Voltage (100V-240V~) 10%
Input Power 300VA
Frequency 50/601Hz

2-2
Specifications

Only fuses of specified specification shall be used.

Fuse Specification: 250V 3.15A D5X20

2.1.3 Environment Requirements


Operating environment, storage environment and running environment
Table 2-3 Overall environment requirements

Operating Environment Storage Environment Running Environment


Requirements Requirements Requirements
Ambient
1030 -1040 1035
Temperature
Relative
20%85% 10%90% 10%90%
Humidity
Atmospheric
70kPa106kPa 50kPa106kPa 70kPa106kPa
Pressure

2.1.4 Specifications
Measurement mode
Two measurement modes are provided: CBC and CBC+DIFF.

Sample mode
Three sample modes are provided: whole blood mode, prediluted mode and Capillary Whole
Blood mode.
Each of the three sample modes can be used in both CBC and CBC+DIFF measurement mode.

Measurement speed
BC-5000's testing speed for OV-WB/OV-PD/OV_PWB modes is not lower than 40 samples
per hour.
BC-5150's testing speed for OV-WB/OV-PD/OV_PWB modes is not lower than 60 samples
per hour.

2.2. Testing Parameters


The analyzer provides quantified results for 25 report parameters and 4 research
parameters(as shown in the table below), 3 histograms and 1 scattergram, and 2 measurement
modes (CBC and CBC+DIFF).
Parameter Series English Name Abbreviation CBC CBC + DIFF

2-3
Specifications

White Blood Cell count WBC


Basophils number Bas# /

White blood cell parameters (15 items), including 4 research parameters


Basophils percentage Bas% /
Neutrophils number Neu# /
Neutrophils percentage Neu% /
Eosinophils number Eos# /
Eosinophils percentage Eos% /
Lymphocytes number Lym# /
Lymphocytes percentage Lym% /
Monocytes number Mon# /
Monocytes percentage Mon% /
Abnormal Lymphocytes ALY# /
number
Abnormal Lymphocytes ALY% /
percentage
Large Immature Cells LIC# /
number
Large Immature Cells LIC% /
percentage
Red Blood Cell count RBC
Hemoglobin Concentration HGB
Mean Corpuscular Volume MCV
Red blood cell parameters (8 items)

Mean Corpuscular MCH


Hemoglobin
Mean Corpuscular MCHC
Hemoglobin Concentration
Red Blood Cell Distribution RDW-CV
Width - Coefficient of
Variation
Red Blood Cell Distribution RDW-SD
Width - Standard Deviation
Hematocrit HCT
Platelet count PLT
parameters (6 items)

Mean Platelet Volume MPV


Platelet Distribution Width PDW
Platelet

Plateletcrit PCT
Platelet larger cell ratio P-LCR
Platelet larger cell count P-LCC

Histogram
English Name Abbreviation CBC CBC + DIFF
White Blood Cell Histogram WBC Histogram
Red Blood Cell Histogram RBC Histogram

2-4
Specifications

Platelet Histogram PLT Histogram

Scattergram
Name Abbreviation CBC CBC + DIFF
Differential Scattergram Diff Scattergram /

means available in this measurement mode, and /means not available in


this measurement mode.
ALY%, LIC%, ALY# and LIC# are research parameters which are only intended
for research purpose and can not serve as basis for clinical diagnosis. For more
information on research parameters, please refer to 5.2.2 General Settings Research
Parameters.

2.3. Performance Requirements

2.3.1 Background/Blank Count


Background refers to the background count performed automatically by the analyzer
during the startup process; its result shall meet the requirements in the following table.
The blank count requirements apply to both whole blood and predilute modes.
Blank count test method: run diluent on the analyzer for 3 times in a row, the highest value
among the 3 results shall meet the requirements in the following table.

Table 2-4 Background/blank count requirements

Parameter Background/blank count requirements


WBC 0.20 109 / L

RBC 0.02 1012/ L

HGB 1g/L

HCT 0.5 %

PLT 10 109 / L

2.3.2 Carryover
Carryover refers to the transfer of blood cells from high concentration sample to low
concentration sample.
Verification method:
Prepare a high concentration sample (centrifugated high value control or

2-5
Specifications

special high value linearity control) which is within the range specified in Table
13, mix and then test it for 3 consecutive times, the test results are i1, i2 and
i3; prepare a low concentration sample (diluted low value control, dilution ratio:
1:10) which is within the range specified in Table 13, test it for 3 consecutive
times, the test results are j1, j2 and j3. Calculate the carryover according to
the following equation, the result shall meet the requirements in Table 12.

Table 2-5 Carryover Requirements

Parameter Carryover
WBC 0.5
RBC 0.5
HGB 0.6
HCT 0.5
PLT 1.0

Table 2-6 Sample Concentration Range of Carryover Test

Parameter Unit High concentration Low concentration


range range
WBC 9
10 /L > 15.00 < 3.00
12
RBC 10 /L > 6.00 < 2.00
HGB g/L > 200 < 40
HCT > 54% <18%
9
10 /L
PLT 9
10 /L > 300 < 100

2.3.3 Repeatability
Test a sample which meets repeatability requirement on the analyzer for 10 consecutive times,
calculate the CV(%) and absolute deviation (d) of each parameter, the results shall meet the
requirements in the following table.

In the equation:
s ---- standard deviation of sample test results;
x ---- mean value of sample test results;

xi ---- actual test result of the sample;

d ---- absolute deviation of the sample test results.

2-6
Specifications

Table 2-7 Whole Blood Repeatability Requirements

Parameter Condition Whole Blood Predilute


Repeatability Repeatability
(CV/absolute (CV/absolute
deviation d) deviation d)
9
4.0010 /L15.00 10 /
9
2.0 4.0
WBC
L
Neu% 50.070.0 4.0(d) 8.0(d)
Lym% 20.040.0 3.0(d) 6.0(d)
Mon% 5.0%10.0 2.0(d) 4.0(d)
Eos% 2.05.0 1.5(d) 2.5(d)
Bas% 0.51.5 0.8(d) 1.2(d)
3.50 1012 / L ~ 6.00 1012 / 1.5% 3.0%
RBC
L
HGB 110 g/L ~ 180 g/L 1.5% 3.0%
MCV 70 fL120 fL 1.0% 2.0%
100 10 / L ~ 149 10 / L
9 9
6.0% 10.0%
PLT
150 109 / L ~ 500 109 / L 4.0% 8.0%
MPV / 4.0% 8.0%

2.3.4 Linearity Range


Linearity was determined by running diluted samples. Samples of different concentrations were
tested in both whole blood and predilute modes; the slope and intercept were calculated per the linear
regression equation, and then the deviation between the theoretical value and test result was obtained,
which shall meet the requirements in the following table.

Table 2-8 Linearity Requirements

Parameter Linearity Range Deviation Range (Whole Deviation Range


Blood) (Predilute)
WBC 0.00 109/L 100.00 0.30109/L or 5 0.60109/L or 6
109/L
(for both BC-5000 and
BC-5150)
100.01 109/L 10 12
500.00109/L
(for BC-5150 only)

2-7
Specifications

RBC 0.001012/L8.00 0.051012/L or 5 0.101012/L or 10


1012/L
HGB 0 g/L250g/L 2g/L or 2 4g/L or 4
PLT 0 109/L 1000 10109/L or 8 20109/L or 16
109/L
(for both BC-5000 and
BC-5150)
1001109/L ~ 5000 12 20
9
10 /L
(for BC-5150 only)
HCT 0%~67% 2% (HCT value) or 4%(HCTvalue) or 6%
3% (deviation percent) (deviation percent)
Note: The linearity ranges above are expressed in both absolute deviation and deviation percent,
meeting either of the ranges are OK.

2.4. Display Range


Table 2-9 Display Range

Parameter Display Range


WBC 0.00109/L999.99109/L
RBC 0.001012/L18.001012/L
HGB 0 g/L300g/L
PLT 0109/L9999109/L
HCT 0%~80%

2.5. Product Description


BC-5150 AUTO HEMATOLOGY ANALYZER composed mainly of analysis unit,
information management unit, output unit and accessories.

The illustrations in this manual are based on BC-5150. The structures of BC-5000
and BC-5150 are basically the same.

2-8
Specifications

This analyzer is heavy and may cause personal injury if handled by only one
person. If necessary, it is recommended to use two person for handling the analyzer. It
is important to follow appropriate safety rules and use appropriate tools while
handling.

2-9
Figure 2-1 Front view of the main unit

1 ---- [Aspirate] key 2 ---- Sample probe


3 ---- Power/Status indicator 4 ---- Display

2-10
Specifications

Figure 2-2 Back view of the main unit

1 --- M-52D diluent inlet 2 --- Waste outlet


3 --- Waste sensor 4 --- Power input socket
5 --- Power switch

2-11
Specifications

Figure 2-3 Left view of the main unit

1 --- Access door 2 --- Panel Module


3 --- Left door assembly 4 --- Network interface and USB port

Main unit
The machine for analysis and data processing is the main part of the product.

Power/Status indicator
The Power/Status indicator is located in the middle of the right side of the analyzer (front
side). It tells you about the status of the analyzer including ready, running, error, sleep and
on/off, etc.

Power switch
The power switch is located on the rear side of the main unit. This switch can be used to
turn on/off the main unit.

To avoid damage, do not turn on/off the power of the analyzer continually in a short
time.

2-12
Specifications

[Aspirate] key
The [Aspirate] key is located on the middle of the right front side (the right one). You can
press the key to start the selected analysis cycle, dispense diluent or wake up the system from
sleep

USB port
There are 4 USB ports on the left side of the main unit for peripheral connection or data
transmission.

2.6. Product Configuration


The system configuration is composed mainly of main analyzer unit, accompanying
accessories and reagent system. The user can choose an optional external scanner/printer.
The USB port can be used to connect the following printer models: EPSON LQ-590K, HP
Laser Jet P1505n, HP Office Jet Pro K5300, and HP LaserJet P1606dn.

2.7. Reagents, Controls and Calibrators


As the analyzer, reagents, controls, and calibrators are components of a system,
performance of the system depends on the combined integrity of all components. You should
only use the Mindray-specified reagents, which are formulated specifically for the fluidic system
of your analyzer in order to provide optimal system performance. Do not use the analyzer with
reagents from multiple suppliers. In such use, the analyzer may not meet the performance
specified in this manual and may provide unreliable results. All references related to reagents in
this manual refer to the reagents specifically formulated for this analyzer.
Each reagent package must be examined before use. Product integrity may be
compromised in packages that have been damaged. Inspect the package for signs of leakage or
moisture. If there is evidence of leakage or improper handling, do not use the reagent.

Store and use the reagents as instructed by instructions for use of the reagents.
When you have changed the diluent, lyses or cleansers, run a background to see if the
results meet the requirement.
Pay attention to the expiration dates and open-container stability days of all the
reagents. Be sure not to use expired reagents.
After installing a new container of reagent, keep it still for a while before use.

2-13
Specifications

2.7.1 Reagents
M-52 D DILUENT

Used for diluting the blood samples to achieve functions such as blood cell counting,
volume measurement and hemoglobin measurement.

M-52 DIFF LYSE

Used for lysing red blood cells to categorize quartile groups of white blood cells.

M-52 LH LYSE

Used for lysing red blood cells to achieve functions such as white blood cell counting,
classification of basophils and hemoglobin measurement.

PROBE CLEANSER

Used for cleansing the instrument regularly.

2.7.2Reagent Consumption Volume


Table 2-10 3107 reagent volume

Diluent DIFF Lyse (ml) LH Lyse (ml) Probe cleanser


Sample Mode
(ml) (ml)
CD 27.5 1 0.2 0
Whole Blood Mode
CBC 23.2 0 0.2 0
CD 27.5 1 0.2 0
Capillary Blood Mode
CBC 23.2 0 0.2 0
CD 27.6 1 0.2 0
Prediluted Mode
CBC 22.9 0 0.2 0
Shutdown 60.9 1 0.2 2
Normal Startup 65 1 0.2 0
Exit Standby 1 3.7 0 0 0
Exit Standby 2 16.9 0 0 0
Exit Standby 3 65 3 0.2 0

2-14
Specifications

2.7.3 Controls and Calibrators


The controls and calibrators are used for calibration and quality control of the analyzer.
The control is an industrial whole blood product, used for monitoring and evaluating the
accuracy of the hematology analyzer. They are available in low, normal, and high levels. The
control is also an industrial whole blood product, used for the calibration of this analyzer to
establish metrological traceability for measurement results. For use and storage of controls and
calibrators, please refer to the instructions for use of the controls and calibrators.
All references related to controls and calibrators in this manual refer to the controls and
calibrators specifically formulated for this analyzer by Mindray.

2.8. Information Storage Capacity


3. Table 2-11 Data storage requirements

Storage BC-5000's storage capacity of sample data is not less than 20000
Capacity BC-5150's storage capacity of sample data is not less than 40000
Storage The storage contents shall include at least the following
Contents information: counting results and diagrams (including histograms
and scattergrams), sample information, patient information,
alarm message, special information of the instrument

2-15
3 Operation Principles

3.1 Overview
This analyzer employs Coulter principle to test the number of RBC and PLT, colorimetric
method to measure the hemoglobin concentration, and semiconductor laser flow cytometry
to obtain differential statistics of white blood cells. The analyzer will calculate the other
parameters based on these results.

3.2 Workflow
The whole system contains the following main functions: reagent system, sample allocation,
sample preparation, sample testing, signal processing, parameter analysis, data management,
status monitoring, scheduling control and information processing, human machine interface,
power supply, cleaning and maintenance. The relationship between these functions are as
shown in the chart below.

The scheduling control and information processing function block controls other function
blocks, which collaborate in accordance with the designed processes and requirements to

3-1
Operation Principles

complete the core task of the whole system, i.e. sample measurement and analysis.

3.3 Sample aspiration


he analyzer will aspire 15L (in CBC+DIFF mode) or 11.5L (in CBC mode) of whole blood
sample in the OV-WB mode.
In OV-PD mode, the operator shall mix 20 L of capillary blood sample and 480 L of
diluent outside the analyzer to obtain a diluted sample with the dilution ratio of 1:25, and
send this diluted sample to the analyzer. In this event, the analyzer will aspirate 200 L of
diluted sample.

3.4 White Blood Cell Measurement


Laser flow cytometry

Figure 3-1 White blood cell measurement

After the blood sample is mixed by the lyse, the red blood cells will be lysed, and the white
blood cells will be dyed. Through the sample probe, the dyed fragments of white blood cell and
red blood cell are injected into the flow cell, which is filled with the diluent. Wrapped in the
sheath fluid formed by the diluent, the cells go through the laser detection zone in rows after a
secondary acceleration. When the cells are exposed to laser beam, the scattered light is related
to the cell size and the refractive index of both the cell membrane and the internal structure.
These scattered light signals are received and converted into electrical pulses by the photodiode.
From these electrical pulses, a two-dimensional distribution map of the cell size and internal
information and be obtained, which is called a scattergram. From the WBC scattergram and
histogram, the white blood cell differential and count can be obtained.

3-2
Operation Principles

3.5 Hemoglobin Concentration Measurement

Colorimetric method
After the diluted sample is added into the lyse, the red blood cells will lyse and release
hemoglobin, which combines the lyse to form hemoglobin complexes. According to the
Lambert-Beer's law, with the radiation of LED monochromatic light with a central wave length of
530 nm, it is possible to measure the transmitted light density of the hemoglobin complexes in
the solution and background, by which the hemoglobin concentration can be calculated.

Hemoglobin concentration parameters


The concentration of hemoglobin (HGB) in g/L can be calculated from the following
equation.

Blank Photocurrent
HGB(g/L) = Constant Ln
Sample Photocurrent
Red blood cell/Platelet measurement

Impedance method
This analyzer employs the impedance method to count the RBC/PLT. There is a small
opening in the RBC bath, which is called inspection aperture. A pair of electrodes on both sides
of the aperture are connected to a constant current power supply. Since the cells are poor
conductor of electricity, when the cells in the diluted sample pass through the aperture under a
constant negative pressure, the resistance between the electrodes changes to generate a pulse
signal across the electrodes, which is proportional to the cell size. The number of the pulses is
equal to the number of cells that pass the aperture, and the amplitude of the pulses is
proportional to the cell size.

3-3
Operation Principles

Figure 3-2 The counting principle

The collected electric pulses are amplified and then compared with the channel voltage
threshold corresponding to the size range of normal red blood cells/platelets, in order to
calculate the number of the pulses which amplitude are within the red blood cell/platelet
channel. Therefore, the collected electric pulses are classified according to the channel voltage
threshold. The numbers of the electric pulses within the red blood cell/platelet channel are the
numbers of the red blood cells/platelets. The size distribution of the cells is determined by the
numbers of the cells in each channel, which are classified according to the pulse voltage
amplitude. The two-dimensional diagram, in which the horizontal axis represents the cell size
and the vertical axis represents the relative number of the cells, are the histogram that reflects
the distribution of the cell groups.

Time Measurement
The basis of time measurement is under certain vacuum pressure and
with fixed aperture dimension, the sample volume passes through the
aperture within a certain period of time is definite. Thus analysis
results can be obtained by calculating sample volume from analysis
duration, dilution ratio, etc. And analysis accuracy can be ensured by
controlling vacuum generating process and monitoring real-time vacuum
pressure and aperture voltage.
Monitor and determine clogging: by monitoring aperture and sample
particle information, relevant characteristic information is concluded,
and the judgment is made in combination with the threshold.

Red blood cell parameters


Red blood cell counts

3-4
Operation Principles

RBC (1012/L) is the number of erythrocytes measured directly by counting the erythrocytes
passing through the aperture.
Mean red blood cell volume
Based on the RBC histogram, this analyzer calculates the mean cell volume (MCV) and
expresses the result in fL.

Hematocrit, mean corpuscular hemoglobin and mean corpuscular hemoglobin


concentration
This analyzer calculates the HCT (%), MCH (pg) and MCHC (g/L) as follows, where the RBC is
expressed in 1012/L, MCV in fL and HGB in g/L.

RBC MCV
HCT =
10

HGB
MCH =
RBC

HGB
MCHC = 100
HCT
where the RBC is in 1012/L, the MCV is in fL, and the HGB is in g/L.

The coefficient variation of the red cell distribution width


Based on the RBC histogram, this analyzer calculates the CV (Coefficient of Variation, %) of
the erythrocyte distribution width.

The standard deviation of the red cell distribution width


RDW-SD (RBC Distribution Width Standard Deviation, fL) is obtained by calculating the
standard deviation of the red blood cell size distribution.

Platelet parameters
Platelet count
PLT (109/L) is measured directly by counting the platelets passing through the aperture.

Mean platelet volume


Based on the PLT histogram, this analyzer calculates the mean platelet volume (MPV, fL).

Platelet distribution width


Platelet distribution width (PDW) is the geometric standard deviation (GSD) of the platelet
size distribution. Each PDW result is derived from the platelet histogram data and is reported as
10(GSD).

Plateletcrit

3-5
Operation Principles

This analyzer calculates the PCT as follows and express it in , where the PLT is expressed
in 109/L and the MPV in fL.

where the PLT is in 109/L and the MPV is in fL.

3.6 Parameter Flags


3.6.1 Flags
The following table lists all 22 flags and their indications.

Flag
Flag Meaning Judgment criterion
Type
Interference of PLT clump or 1. The DIFF and BASO
WBC Abnormal NRBC to WBC count and channels are
differential may exist: unproportionate.
Many scatter-points in the
Immature cells or blasts may
Immature Cell? immature cell area of the
exist
scattergram
Many scatter-points in the
Abnormal lymphocytes or
abnormal/ atypical
Abn./Atypical Lym? atypical lymphocytes may
lymphocytes area of the
exist.
scattergram
Leucopenia Low WBC analysis results WBC < 2.5010^9/L
Leucocytosis High WBC analysis results WBC > 18.0010^9/L
Low neutrophils analysis
Neutropenia NEUT# < 1.0010^9/L
results
WBC
High neutrophils analysis
Neutrophilia NEUT# > 11.0010^9/L
results
Low lymphocytes analysis
Lymphopenia LYMPH# < 0.8010^9/L
results
High lymphocytes analysis
Lymphocytosis LYMPH# > 4.0010^9/L
results
High monocytes analysis
Monocytosis MONO# > 1.5010^9/L
results
High eosinophils analysis
Eosinophilia EO# > 0.7010^9/L
results
Basophilia High basophils analysis results BASO# > 0.2010^9/L
Wbc < 4.010^9/L and Rbc <
Pancytopenia WBC, RBC and PLT low 3.512^9/L and Plt < 100
10^9/L
Possible presence of The distribution of RBC
RBC RBC Histogram Abn.
microcytes, macrocytes, histogram is abnormal

3-6
Operation Principles

anisocytosis, RBC
agglutination and dimorphic
histogram
HGB abnormal or RBC
MCHC > 380 g/L
HGB Abn./Interfere? agglutination, or interference
or HGB interference
may exist (e.g., WBC high)
Microcytosis MCV low Mcv < 70fL
Macrocytosis MCV high Mcv > 110fL
Anemia Anemia HGB < 90g/L
Erythrocytosis RBC high RBC > 6.510^12/L
Possible presence of
The distribution of PLT
PLT Scattergram Abn. microcytes, red blood cell
scattergram is abnormal
debris, giant PLT or PLT clump
PLT
Thrombopenia PLT low PLT < 6010^9/L

Thrombocytosis PLT high PLT > 60010^9/L

3.6.2 Shielding Protocol


See the following table for the shielding relations of each flag.
Flag
Flag Shielding Relation
Type
WBC Abnormal R/Flag parameter: WBC, related DIFF parameters
Immature Cell? R/Flag parameter: Neu#, Mon#, Bas#.
Abn./Atypical R/Flag parameter: Lym#, Mon#, Bas#, etc. Differ
Lym? slightly based on the situation.
Leucopenia /
Leucocytosis /
Neutropenia /
WBC
Neutrophilia /
Lymphopenia /
Lymphocytosis /
Monocytosis /
Eosinophilia /
Basophilia /
Pancytopenia /

R/Flag parameter: RBC, HCT, RDWCV, RDWSD, etc.


RBC Histogram Abn.
Position of the ? differs slightly based on the situation.

RBC HGB Abn./Interfere? R/Flag parameter: HGB, MCH, MCHC.

Microcytosis /
Macrocytosis /

3-7
Operation Principles

Anemia /
Erythrocytosis /

PLT Scattergram Abn. R/Flag parameter: PLT, MPV, PDW, PCT, PLCR, PLCC, etc.

PLT
Thrombopenia /

Thrombocytosis /

3.6.3 Sensitivity Adjusting Mechanism


The sensitivity adjusting mechanism allows adjustment of flag rate to meet the needs of all
hospitals (see Flag alarm sensitivity in the Operators Manual).

3-8
4 Software and Interface

4.1. Start-up

Version Check
At startup, the machine will automatically check the software version. If the software version
does not match, a window will pop up as shown below:

Figure 4-1The software version match window


Please prepare the software upgrade package, select the appropriate language and click
the OK button. The system will perform the software upgrade progress.
Detection of unmatched software version at startup is caused by upgrading individual
software component, such as digital version FPGA chip writing software, drive board FPGA
writing software, MCU writing software, sequence and system software.

Always use controlled software upgrade package for software upgrade.

4-1
Software and Interface

4.2. Login
4.2.1 Log in using service level username and password
Username: "Service"
Password: "Se s700"(there is a space between Se and s700).

The login password is case sensitive.

4.2.2 Login Check


After login with service level password, the machine will automatically check the board backup
data and SD card data. If any board or SD card has been changed, or abnormal shutdown occurs after
configuration, a recovery or backup operation of the instrument data will be prompted after login with
service level password.

Figure 4-2Login check prompt window


Click OK to enter the Data Backup and Recovery screen, and perform data backup and
recovery according to the prompts.

4-2
Software and Interface

Figure 4-3 Data Backup and Recovery screen


1) If a new SD card is replaced, please follow the subsequent text guide and select Restore to
restore the important parameters to the SD card.
2) If the MPU is replaced, please follow the subsequent text guide and select Backup to back
up the data in the new MPU.
3) If abnormal shutdown occurs after configuration has been changed, please select Backup to
back up the data in the new MPU.

4-3
Software and Interface

4.3. Review

4.3.1 Stability Trend Graph

Figure 4-4 Stability trend graph screen


The stability and the limit of the RBC and PLT particles are considered to be 15% of the mean
by default; the stability and limit of other particles are considered to be 10% if the mean by default;
the limits of the fluids pressure, RBC aperture voltage, HGB voltage and vacuum in the vacuum
chamber are considered to be 10% of the mean by default.
The limit can be set manually; the data length is 6 digits;
If the limit is larger than the mean, the total low limit is 0.

4-4
Software and Interface

4.3.2 Trend Graph

Figure 4-5 Trend Graph Screen


If the mean of the parameter can be gained by calculation of the selected data, then the y-axis of
mean central line, upper limit data line, lower limit data line are as follow by default: Mean, Mean +
Mean * 10%, Mean Mean * 10%;
If the results of the Mean+ deviation do not meet the requirements of the data format of the
parameter, then obtain the y-axis of the upper and lower data line by the rounding-off principle.
If the limit is larger than the mean, set the low limit as 0;
If the upper limit exceeds the parameter display range, click the OK button, the OK dialog
box will promptThe upper limit exceeds the parameter display range, please reset.

4.4. Calibration

4.4.1 Calibration Factor and Transfer Factor


The purpose of calibration is to obtain accurate blood analysis results.
The calibration method is multiplying the result by the calibration factor, so that the final
analysis result is close to the target. The calculation equation of the calibration factor is:

4-5
Software and Interface

There are two different analysis modes, CBC+DIFF and CBC. The two analysis modes
respectively correspond to two fluidics sequence. Therefore, the analysis results of the same sample in
different modes are different. However, this difference is relatively fixed. During calibration, it is
only required to obtain the calibration factor of one mode. The calibration factor of the other mode
can be calculated by multiplying this fixed difference coefficient, which is called the transfer factor.
The calculation equation of the transfer factor is:

There are two different sample modes, whole blood mode and prediluted mode, which also
correspond to different fluidics sequence. Therefore, different sample modes need to be calibrated
separately.
The calibration factors can be classified as factory calibration factor and user calibration factor.
For the CBC+DIFF mode, the analysis result will be calculated by the following equation:

Analysis result=measurement value factory calibration factor user calibration factor


For the CBC mode, the analysis result will be calculated by the following equation:
Analysis result=measurement value factory calibration factor transfer factor user
calibration factor
The calibration will only generates calibration factors and transfer factors of five traceable
parameters: WBC, RBC, HGB, MCV and PLT.

If login with service level password, the calibration will modify the factory
calibration factor and transfer factor, and will modify the user calibration factor to
100.00%.

4-6
Software and Interface

4.4.2 Calibration

Figure 4-6Service level calibration screen


The service level calibration with calibrators will generate the factory calibration factor and
transfer factor at a time. The first 6 counts are performed in CBC+DIFF mode, and the last 6 counts
are performed in CBC mode. After all the 12 counts are completed, the new calibration factor and
transfer factor will be automatically calculated. The operator will be prompted to save the calibration
factor when exiting this screen.
Before the calibration, be sure to set up the Calibrator Lot No., the Calibrator Exp. Date,
Analysis Mode and Calibration Targets.
The range of Calibration factor is [0/75, 1.25]. .

Please use specified calibrators for calibration before their expiration date.

If the calibration factor and CV are beyond the above range, they will be displayed in
red, and the current result will not be saved.

4-7
Software and Interface

4.5. Sample Probe Debug


The purpose of sample probe debug is to check if the probe can move to each working position
properly.

Figure 4-7Sample probe debug screen


Enter the sample probe debug screen and click the Initial position button. Wait until the
initialization is completed before start the sample probe debug. For detailed information, please refer
to Section 12.1 Mechanical position debug in Chapter 12.

4.6. Temperature Calibration


The purpose of temperature calibration is to minimize the difference between the measurement
and the actual temperature in order to ensure the accuracy of sample analysis.

4-8
Software and Interface

Figure 4-8Temperature calibration screen


There are four quantities in this screen: total difference, new difference, machine measurement
value and meter measurement value. This screen does not include one quantity: the actual
measurement value, which is the actual temperature measured by the temperature sensor. These
quantities satisfy the following equation:
New difference = Meter measurement value Actual measurement value
After clicking Save, the new difference is assigned to the total difference: Total difference =
New difference
Machine measurement value = Actual measurement value + Total difference

4.7. Gain Calibration


Gain calibration includes gain calibration of the optical channel, the impedance channel and
HGB. The purpose of optical channel gain calibration is to ensure the accuracy of the algorithm
categorization, and the purpose of impedance channel and HGB gain calibration is to ensure accurate
values of MCV and HGB to be calculated. If the scattergram is normal but there is no categorization
results or obvious deviation in the categorization results, check the optical system or recalibrate the
optical gain. If the HGB background voltage in the status screen is beyond the range, recalibrate the
HGB gain.

4-9
Software and Interface

Figure 4-9 Service level calibration screen


The optical gain, MCV gain and HGB gain can be calibrated simultaneously in the Gain
Calibration screen. As a software gain, the optical gain can be calibrated by mean method. Selecting
the Select box of the single count to involve this count in the mean calculation. As hardware gains,
the MCV gain and the HGB gain can be calibrated by successive approximation, and the Select box
has no effect on the results of the MCV gain and HGB gain.
Whether the gain calibration is successful is marked by color. Unsuccessful calibration result
will be displayed in red, which means a recalibration is necessary.
If a target has not been set up, then the corresponding gain factor will not be calibrated, as
G.S. MCV in the figure above.
Note: The G.S. MCV target is not the same as MCV calibration target, they are not the
same value.
When exiting the Gain Calibration screen, the operator will be prompted whether to save the
gain factor.

Only calibration with calibrators is supported in the Gain Calibration screen.

4-10
Software and Interface

Please use specified calibrators for gain calibration before their expiration date.
For the targets, please refer to the calibrator target sheet.

4.8. Gain Setting


The optical gain, MCV gain and HGB gain can be set up in the Gain Setting screen. The purpose
of gain setting and gain calibration is the same. Gain setting can be considered as manual gain
calibration.

Figure 4-10 Gain Setting screen


The optical gain is a software gain and shall be filled with the gain factor, which can be
calculated by the following equation:

The gain factor is a percentage value. The Gain Setting screen allows the gain factor to be set as
accurate to two decimal places.
The MCV gain and HGB gain are software gains, which require the digital potentiometer to be
set. The range of gain setting is [0, 255].
The MCV gain setting can be calculated by the following equation:

4-11
Software and Interface

The HGB gain setting does not need to be calculated by an equation. Just modify the
setting until the background voltage is equal to 4.5V.

The gain settings will have effect on the affectivity of the measurement. Please be
careful with the setting.
While the analyzer is in standby, the HGB voltage will not reflect the background
voltage. In this event, the operator must exit the standby mode before adjusting the HGB
gain.

4.9. Performance

4.9.1 Background test

Figure 4-11 Background Count screen


In the Background Count screen, pressing the aspirate key without using any sample will start
the background count. In the Background Count screen, if pass is displayed in the Result column,

4-12
Software and Interface

then the background test is passed.

4.9.2 Reproducibility

Figure 4-12 Reproducibility Test screen


Select the samples that satisfy the reproducibility range and perform 10 consecutive
measurements on the analyzer. Calculate the CV (%) or absolute deviation d of each measured
parameter and check if the reproducibility requirement is met.

Normal controls are usually used for reproducibility application at the client end.

4-13
Software and Interface

4.9.3 Carryover

Figure 4-13 Carryover Test screen


Test method: Under the stable condition of the analyzer, perform three consecutive
measurements on the high-level sample immediately followed by three consecutive
measurements on the low- level sample. The carryover can be calculated from the following
equation:

First low - level sample resultThird low - level sample result


Carryover (%) = 100
Third high - level sample resultThird low - level sample result
Advanced Toolbox

4.9.4 Reference range of normal samples


Deviation
Parameter Mean
Range
NEU_LAS_P 139.91 6.01
NEU_MAS_P 123.87 9.84
NEU_WAS_P 39.40 4.80
NEU_EQWIDTH_P 14.57 0.30
NEU_LAS_CV(%) 6.83 3.15
NEU_MAS_CV(%) 12.95 2.35
NEU_WAS_CV(%) 20.88 2.35
NEU_EQWIDTH_CV(%) 1.12 0.65

4-14
Software and Interface

4.10. Advance Toolbox

4.10.1 System Configuration

Figure 4-14System Configuration screen


In the System Configuration tab of the advanced toolbox, you can modify the language type, the
analyzer serial No. and data type to be saved.
After the Save inf file option is selected, the inf file will be saved to directory
/mnt/hd/soft/dat/rawdata. The inf files save the analyzer status and sample analysis data for
diagnostics of analyzer performance.
After the Save raw data option is selected, the dat file will be saved to directory
/mnt/hd/soft/dat/dat. The dat files save the raw pulse signals collected by the MPU FPGA for
diagnostics of analyzer performance.

The modification to language type will not take effect until after the analyzer is
restarted.
The saved inf files and raw data files will occupy relatively large amounts of disk
(SD card) space.

4-15
Software and Interface

At most 500 inf files can be saved. After 500 files are saved, the new files will
overwrite the old files.

4.10.2 One-key Export

Figure 4-15One-key Export screen


The contents that can be exported by One-Key operation include:

Inf files

Special information files

Analyzer information: includes version information, configuration parameter (gain and


calibration), algorithm parameters, analyzer status, software language and analyzer name

Software debug information: includes parameter setting, error log, upgrade log and system
log.

Closed-reagent information: includes closed-reagent information and counter information

The USB flash drive has been pre-formatted as FAT32.


The following USB drive models are recommended: Kingston 8/16G, SanDisk 8/16G,

4-16
Software and Interface

Maxell 4/8G.
There is enough free space in the USB drive. It is recommended to reserve 4G space.

4.11. Software Update


Create an upgrade USB drive

Unzip update.rar and copy the unzipped update directory to the root directory of the
USB drive.

The USB flash drive has been pre-formatted as FAT32.


The directory structure of USB drive: the root directory contains a directory named
update, which contains two directories: step1 and step2.

Upgrade

Insert the USB drive into the USB port on the analyzer. Enter the Advanced Toolbox
and launch Upgrade to upgrade the software according to the prompts. The upgrade process can
be divided into two steps.

Step 1: upgrade the guidance and operation system,

Step 2: upgrade the software components.

When upgrading the boot and operation system, the operator will be prompted to
restart the analyzer between step 1 and step 2. When upgrading the software components, the
upgrade process will directly go to step 2.

Never disconnect the USB drive or the power supply during the upgrade process.
Otherwise the analyzer may not be able to start.

The duration of the upgrade process varies with the upgrade contents. Typically it
will last for around 10 minutes. Interaction is needed during the process. Please do
not leave when upgrading the analyzer.

4-17
Software and Interface

Troubleshooting

If the upgrade fails, try again.

4.12. Status Indicator


The system status is indicated by the three color indicator on the panel door. All the flash cycles
are 2 seconds. The indicator changes with the analyzer status as shown in the table below:
Table 4-1 Indication of the main unit status indicator
Analyzer Status Indicator Comments
Ready Green light on Sequence is allowed
Running Green light flashing Sequence is being performed
Running with error Red light flashing An error is present and the
system is running
Stop with fault Red light on An error occurs and the
system is not running
No fault is present, but Yellow light on Initialization and standby
fluidics action is not status of sequence is not
allowed involved in the startup
process
Enter/Exit standby Yellow light flashing Enter/Exit standby status
status

4.13. Buzzer
When an error occurs, the buzzer will beep. The alarm will be automatically cleared by tapping
the touchscreen or correcting the error. The buzzer alarm will stop when all the error are cleared. It
prompts to instruct the user with possible actions by the beep.
Table 4-2 Main unit buzzer prompts
Event Buzzer Comments
Prompts
Startup completed One short beep Startup completed means the
whole startup process has been
completed and the analyzer is
ready for operation
Open-vial aspiration completed Two short beeps
When count operation can not be One long beep If these screens have already

4-18
Software and Interface

started in count related screens prompted, then the buzzer need


(including Sample Analysis, QC, not be responded again.
Calibration, Reproducibility,
Carryover, Background, Aging and
Optical Gain Calibration Count, etc.),
press the aspirate key
Error Long intermittent Tap the touchscreen to stop the
beeps buzzer
Analyzer is ready One short beep Analyzer enters ready status
from other status
When the screen is black prompting Silence If an error occurs during the
Please power off the analyzer shutdown process, the buzzer
will beeping when the screen is
black

4-19
5 Data Transmission
5.1. LIS Connection
Communication setup (Menu>Setup>System Setup>Communication Setup)

The operator can perform the following setups in the Communication Setup screen
Protocol setup

Transmission mode

Figure 5-1 Communication Setup screen


Protocol setup

IP address:
The IP address setting of the analyzer defaulted as 10.0.0.2
Subnet mask:
The subnet mask of the analyzer. A typical subnet mask is 255.255.255.0
Default gateway:
IP address of the gateway.
Mac address:
The Mac address of the analyzer, given by the factory. Can not be changed.
Comm. Protocol:
For selecting the protocol type. Click the pull-down list and select the appropriate
communication protocol type from the options.

5-1
Data Transmission

ACK synchronous transmission:


This function can be activated by selecting the ACK synchronous transmission
checkbox.
When this function is active, the ACK overtime is defaulted as 10 seconds. The
operator can adjust this value in the edit box.

The IP address of the analyzer is statically allocated. Before setup, please consult
your network administrator to avoid IP conflict.
For communication across subnets, the subnet mask and the gateway must be correct.
Please consult your network administrator.

Transmission mode

The operator can select required options by clicking the following checkboxes to activate
corresponding communication setup as needed:
Auto retransmit
The Auto retransmit can only be selected when the ACK synchronous
transmission is selected, if ACK is not selected after overtime, the software will
transmitted the content that has transmitted before automatically.
Auto communication
If it is selected, the software will transmit the sample information and count
result to the LIS automatically after the sample analysis.
Transmit as print bitmap data
If it is selected, the scattergram and histogram that transmitted to the LIS are the
same as the print graph, whose background are white.
Transmission method for histograms and scattergrams

Click the pulldown list and select the transmission method for histograms and scattergrams
as required from the following options:
Not transmitted
If it is selected, the graphic and image data will not be transmitted.
Bitmap
If it is selected, the image data will be transmitted, all the scattergram and
histogram of the LIS received are bitmap.
Data
If it is selected, the graph data will be transmitted during the transmission, the
scattergram and histogram that LIS received is the data, the data format is customized;
it can only be viewed by the Mindray data management software.

5-2
Data Transmission

5.2. Data Management Software Setup


Communication parameter setup

Figure 5-2 Communication Parameter Setup screen for data management software

As illustrated above, IP Address refers to the analyzer IP address, Port is fixed as 5100, and
Protocol shall match the analyzer configuration.
Communication instrument management

5-3
Data Transmission

Figure 5-3 Communication Instrument Management screen for data management software

The analyzer is the server, and the LIS and data management software is the client. The
connection needs to be initiated by the client.

5.3. Communication Error Analysis

5.3.1 Physical Connection


Check if the network cable works properly and if the physical network connections are
correct.

5.3.2 Communication Setup


Check if the network setup is correct, including the communication setup of the analyzer
and LIS.

5.3.3 Network Firewall


Please open the network connection license of LIS and data management software, and
open the network connection license of port 5100 to check for the firewall.

5-4
6 Optical System

6.1. Introduction to the Principles of Optical System

6.1.1 Operation Principles


The basic principle of optical system is the employment of flow cytometry-based laser scattering
method. As shown in Figure 6-1, wrapped in the diluent sheath, the processed and diluted blood
forms a sample stream carrying cells. This sample stream becomes very thin due to the focusing
effect of the sheath, forcing the cells to flow one by one through the center of the chamber with a
certain space between each other. Elongated elliptical Gaussian beam goes through the optical zone of
the flow cell, irradiates individual cells to generate scattered light, which is received by the detector
and transformed into photoelectric signals necessary for cell counting and categorizing.

Figure 6-1 Laser scatter principle

Since the sample stream has a certain width, different cells will pass the beam zone at slightly
different positions. In order to ensure the consistency of cell scatter signals, the Gaussian beam shall
has a certain width in the direction perpendicular to the cell movement to minimize the density
variation of the beam coving the sample stream, as shown in Figure 6-1. Meanwhile, in order to
prevent the beam from radiating several cells at the same time, the beam shall be small enough in the
direction of the cell movement, just being able to cover the entire cell. Therefore, the beam used for
radiating the sample stream shall be an elongated elliptical beam.

6-1
Optical System

6.1.2 Beam Path of the Optical system


As a component of the optical path, the optical zone of the flow cell is the zone that the laser
beam passes which are shown as follows:

Light source assembly: Beam shaping function component, which shapes the divergent elliptical
beam generated by the semiconductor laser to elongated beam and directs it into the flow cell.
Flow cell assembly: Both an optical component and a fluidic component. As the fluidic interface
of the optical system, it provides stable sample streams. The optical performance of this component is
also critical. Dirt, contamination or dust in the optical zone on the inside and outside surface of the
flow cell may have great impact on the performance of the optical system.
Scattering detection assembly: consists of aperture and photoelectric sensor. Used for collecting
the scattered light generated by the cells. There are three photoelectric sensors in the optical system
for collecting scattered light of three angle ranges, including Low Angle Scatter (LAS), Medium
Angle Scatter (MAS) and Wide Angle Scatter (WAS).

Figure 6-2 Optical path diagram of the optical system


The relative positional relationship between the three parts of the optical path is achieved by
precise commissioning and fastening with special instruments, and therefore is not field serviceable.

6.2. Physical Structure

6.2.1 Overall Structure


The overall structure of the optical system is shown in Figure 6-3. According to different
functions, the system can be divided into the following parts:

6-2
Optical System

Figure 6-3 Overall structure model of the optical system

1 ---Light source assembly 2 ---Flow cell assembly


3 ---Substrate assembly 4 ---Preamplifier and shield shell
5 ---Shield assembly 6 ---Scattering detection assembly
7 ---Laser control board

Figure 6-4 Physical structure of the optical system

6-3
Optical System

6.2.2 Light Source Assembly


The light source assembly is used for providing light source output and beam shaping for the
optical system, as shown in Figure 6-5. Both the removal of internal parts of the light source assembly
and the removal of the assembly from the substrate are forbidden. Generally, if the light source
assembly is determined to be faulty, the whole optical system shall be replaced.

Figure 6-5 Light source assembly

6.2.3 Flow Cell Assembly


The flow cell assembly is the fluidic interface of the optical system. The fluidics are turned into
stable sheath stream under the pressure of the shield fluid bath. After reaction, the cells are injected
by the sample syringe into the flow cell assembly and are wrapped by the sheath. Then the cells go
through the flow cell one by one for laser irradiation, as shown in Figure 6-6.

(a) Physical view of the flow cell assembly (b) Operation principle of the flow cell

6-4
Optical System

Figure 6-6 Flow cell assembly

Both the removal of internal parts of the flow cell assembly and the removal of the assembly
from the substrate are forbidden. Generally, if the flow cell assembly is determined to be faulty, the
whole optical system shall be replaced.

6.2.4 Optical Substrate Assembly


The substrate assembly provides support, fixation and shock absorption for the optical system, as
shown in Figure 6-7. The screws in the substrate assembly is not removable. If loose screws result in
abnormal condition of the optical system, please replace the whole optical system.

Figure 6-7 Optical substrate assembly

6.2.5 Preamplifier and Shield Shell


The preamplifier and shield shell are used for pre-amplifying the optical system signals and
shielding signal noise. The preamplifier is connected by the preamplifier shield shell with the optical
system shield shell. If the preamplifier is determined to be faulty, it is possible to remove and replace
it from the optical system, as shown in Figure 6-8.

6-5
Optical System

Figure 6-8 Preamplifier and shield shell

6.2.6 Shield Shell Assembly


The shield shell is used for isolating the optical system from the outside to avoid interference of
dust, stray light and electromagnetic noise, and for connecting the optical system to the analyzer, as
shown in Figure 6-9.

Figure 6-9 Shield shell assembly

6.2.7 Scattering Detection Assembly


As the detection unit of the optical system, the scattering detection assembly consists of
medium/small angle PD and large angle PD and corresponding medium/small angle aperture and

6-6
Optical System

large angle aperture and support structure. The 3D model and physical view are shown in Figure 6-10.
The scattering assembly is a whole unit and can not be removed from the optical substrate, although
the large angle PD assembly is removable. When the scattering detection assembly is determined to
be faulty, it is necessary to replace the whole optical system.

Figure 6-10 Scattering detection assembly

6.2.8 Laser Control Board


The laser control board is used for providing stable driving current for the laser to keep a full and
stable laser output power, as shown in Figure 6-11. If the laser control board is determined to be
faulty, it is possible to remove and replace it from the optical system.

Figure 6-11 Las

6.3. Determinating the Optical System Status


When abnormal sample scattergram causes failed or incorrect categorization, and the reagent and
fluidic connections are determined to be normal, it will be necessary to check the optical system
status. In this section, the optical system status is tested with standard particles. Prior to the test,
prepare the following items:
Consumables: 4k-07 Std. part. (7m) and 1.5 mL centrifuge tube, slobe cleanser.
The detailed procedure is as follows.
1. First, add 0.5 mL of deionized water to a 1.5mL centrifuge tube. Shake the 7 m std.

6-7
Optical System

part. bottle until the solution is well mixed, then add 3 drops of the solution in to the
1.5mL centrifuge tube. Cap the tube and shake it until this solution become well
mixed, as shown in Figure 6-12.

Figure 6-12 Preparation of the 7m std. part. solution

2. Select the Maintenance menu and enter the Optical screen. Perform counting with
the prepared std. part. solution. After the counting is completed, the result will be
automatically displayed in the screen, as shown in Figure 6-13.
3. Determine the optical system status by the parameter Particle 1 according to the
counting result. The optical system is OK if all the following requirements are met:
Parameter Total CG Position CV
LAS 38~45 6.50
MAS 1500~3000 100~158 3.00
WAS 100~200 8.00
4. Generally, if the parameters fail to meet the requirements, it may be caused by a dirty
flow cell. The built-in maintenance program can be used to solve this problem. The
method is as follows: click the Maintenance-->Maintenance menu and select Flow
Cell Cleaning in the Cleaning screen. The machine will automatically complete the
cleaning operation (in about 1 minute).
After the maintenance, repeat step 2 and 3 and check if the result meets the requirements. If not,
probe cleanser should be used to clean the flow cell: click the Maintenance-->Maintenance menu
and select Overall Soak in the Maintenance screen, and carry out the operation of the program.
After the maintenance above, repeat step 2 and 3 again, and check if the result meets the requirements.
If still not, the manual maintenance is required. For details, please see the next chapter.

6-8
Optical System

Figure 6-13 Optical screen of the stanadard particle

6.4. Maintenance and Replacement of the Optical


System
When the optical system is determined as abnormal according to Section 6.3, and can not be
restored by automatic maintenance, then manual maintenance will be necessary. Furthermore, if the
optical system is determined as normal according to Section 6.3, but the scattergram or categorization
of the blood sample is abnormal, then other problems except the optical system shall be taken into
account.
Before performing maintenance and replacement on the optical system, prepare the following
items:
Tools: A crosshead screwdriver and an allen wrench set.
Consumables: 4k-07 Std. part. (7m), 1.5 mL centrifuge tube, microfiber clean cloth,
dehydrated alcohol and probe cleanser.

6.4.1 Maintenance of the Optical System


Before maintenance of the optical system, it is necessary to open the top cover of the optical
system shield shell. As shown in Figure 14, use a crosshead screwdriver to remove the locking screw.
Open the top cover carefully to reveal the internal structure of the optical system, as shown in Figure
6-4.

6-9
Optical System

Tap "Status" > "Voltage&Current" in the menu to enter the Voltage&Current screen, the
laser will illuminate automatically.

Figure 6-14 Location of the screws on the shield shell

Precautions before proceeding to the next step are as follows:

Never look directly into the laser with eyes or through an optical instrument;
During test of optical system with the top cover open, please shelter the optical system to
prevent bright environmental light from irradiating the detector inside the optical system.
Generally, the following steps can be followed to determine which part needs to be maintained.

1. Check if the wires are firmly connected inside the optical system and if the optical path is
blocked by wires.
2. Check if the output spot is normal. Place a small piece of white paper near the output exit
and observe the light spot. The ideal spot shape is a vertical ellipse which is clipped at both
top and bottom, as shown in the left in Figure 6-15. The actual spot is shown in the right in
Figure 6-15 with a faint halo around it.

6-10
Optical System

Figure 6-15 Light spot at the output exit

Abnormal spots can be different, including dark spot, spot clustered into a dot, spot with dark
lines, spot with a scattered halo, seriously damaged spot, spot with multiple dark dots in the center, as
shown in Figure 6-16.

Figure 6-16 Example of abnormal output spots

Abnormal light spots are usually caused by damage or contamination of the laser control board,
the laser or the lens in the light source assembly.
Missing parts, dark lines or dark dots in the spot are usually caused by contaminated lens in the
light source assembly. A clean cloth dampened with dehydrated alcohol may be used to wipe the lens
gently, spiraling outward from the center. Be careful not to touch the interior of the lens barrel.
If the light spot disappears, darkens or diverges, then the laser control board may be damaged.
After the problem is determined, replace the laser control board (be aware of electrostatic) separately
and adjust the variable resistor on the board. Perform a std. part. test in accordance with 6.3 to ensure
the std. part. indicators to meet the requirements. After replacement, a gain calibration in accordance
with Section 4.6 as well as a calibration in accordance with Section 4.3 shall be performed on the
optical system.

6-11
Optical System

If the light spot disappears, converges, darkens or diverges, and the laser control board is
determined to be in good condition, then the laser could have been burned. Since the light source
assembly can not be replaced separately, it will be necessary to replace the optical system.

3. Observe the light spots on the surface and inside the flow cell from the following angles, as
shown in Figure 6-17. If the exterior of the flow cell is very bright, then the exterior may be
stained. Use a clean cloth dampened with dehydrated alcohol to wipe the exterior, until the
bright part darkens or disappears, as shown in the right in Figure 6-17. If the interior of the
flow cell is very bright and can not be darkened by the built-in cleaning procedure, then it
will be necessary to manually rinse the inside of the flow cell.

Figure 6-17 Flow cell before and after cleansing

The cleansing procedure is as follows: Prepare a syringe with a 100 mm long Teflon
tube, a 200 mm long Teflon tube and probe cleanser, as shown in Figure 6-18. Shutdown
the analyzer, remove the tubes at the waste outlet and the sheath inlet of the flow cell.
Connect the syringe to the waste outlet. Connect one end of the other Teflon tube to the
sheath inlet, and put another end into the probe cleanser. Draw the probe cleanser with the
syringe until the probe cleanser enters the syringe. At this time, the flow cell will be filled
with the probe cleanser. After soaking for about 10 minutes, use clean water instead of the
cleanser. Draw the syringe forcefully for 2 to 3 times, until the bright spots in the flow cell
darken or disappear.
Connect the optical path and the tubes and verify the connection, and then turn on the
analyzer power.

6-12
Optical System

Figure 6-18 Preparation before cleaning the flow cell interior

4. Check if the output light is perpendicular to the flow cell. Place an inner hexagon spanner
at the output exit. The output spot and the spot reflected by the flow cell shall appear
simultaneously and strictly coincide with each other, as shown in Figure 6-19.

Figure 6-19 Output spot and reflection spot


After the maintenance is completed, verify if the optical system is working correctly in
accordance with the steps in Section 6.3:

If the std. part. CV meets the requirement, but the CG position is too low or too high,
slightly adjust the variable resistor on the laser control board as shown in Figure 6-11, until
the CG position meets the requirement. After maintenance according to this procedure, a
gain calibration in accordance with Section 4.6 as well as a calibration in accordance with
Section 4.3 shall be performed on the optical system.
If the CG position meets the requirement but the CV doesn't, then the maintenance is not
qualified. Perform another check and maintenance in accordance with this section.
If the CG position and the CV can not meet the requirements after the maintenance, then it
will be necessary to replace the optical system.

6-13
Optical System

6.4.2 Replacement of the Optical System


If the problem is not solved after maintenance, or if the problem is not serviceable, then it is
necessary to replace the optical system with a new one. The replacement procedure is as follows:

1. Power off the analyzer.


2. First, gently disconnect the waste outlet tube from the optical system, and connect to a
syringe. Disconnect other tubes, aspirate remaining fluid with the syringe from the system.
Unscrew the screws and disconnect the signal line from the optical system, as shown in
Figure 6-20.
Note: Follow the procedure and prevent the corrosive fluid from falling onto the
frame!

Figure 6-20 Remove the optical system

3. Remove the optical system carefully and short the tubes of the optical system in accordance
with Figure 6-21.

6-14
Optical System

Figure 6-21 Short the tubes of the optical system together

4. Install the new optical system into the analyzer. Connect the signal wires, fluidics and
optical path. Turn on the analyzer and verify the status of the new system in accordance
with the steps in Section 6.3.
Note: The fluidic interfaces of the optical system are very fragile. Be careful to avoid
knocking them when connecting the tubes during installation!

5. Perform a gain calibration in accordance with Section 4.6 as well as a calibration in


accordance with Section 4.3 on the optical system.

6-15
7 Fluidics
7.1. Measurement Flow
The fluidics of the analyzer can be divided into two measurement channels:
WBC&HGB Channel
RBC&PLT Channel
The system flowchart of the WB-CBC+DIFF mode is shown below (DIFF lyse and DIFF
sample configuration & measurement are not available in CBC mode):

Figure 7-1Fluidics flowchart (WB-CD mode)

7-1
Fluidics

The system flowchart of the PD-CBC+DIFF mode is shown below (DIFF lyse and DIFF sample
configuration & measurement are not available in CBC mode):

Figure 7-2Fluidics flowchart (PD-CD mode)

7.1.1 WBC&HGB Channel


DIFF Measurement
Reagents:

7-2
Fluidics

DIFF reagent: used for lysis of red blood cells and specialization of different white blood
cells;
Diluent: background solution used for providing sheath fluid and cleansing
Measurement principle: flow cytometry and laser scatter

Measurement parameters: MONO#, MONO%, LYMPH#, LYMPH %, NEUT#,


NEUT%, EOS#, EOS%

Graphics: 4 Differential scattergram

Dilution ratio: 1:105.8

Counting duration: 9.1 s

Counting flow: 0.008575417ml/s

Counting volume: the flow of the sample stream is constant, which can be
converted to counting volume by controlling the counting duration

Function description: Mix 15 L of blood sample and 550 L of diluent in the


WBC bath. After the secondary aspiration, add 1 mL of DIFF lyse. After the reaction
has been on for a certain time, place the prepared sample at the bottom end of the
flow cell. The sample will be wrapped by a sheath stream generated by the large
volume syringe of the syringe linkage and be pushed by the small volume syringe
into the flow cell for the measurement.

WBC Count and BASO Measurement


Reagents:

LH lyse: used for lysing red blood cells and platelets and separating the basophiles by
volume from the other white blood cells
Diluent: used for providing sheath fluid and cleansing
Measurement principle: flow cytometry and laser scatter

Measurement parameters: WBC, BASO# and BASO%

Graphics: WBC histogram


1
Dilution ratio: 1:123.8*

Counting duration: 11.5 s

Counting flow: 0.008575417ml/s

Counting volume: the flow of the sample stream is constant, which can be
converted to counting volume by controlling the counting duration

Function description: After DIFF reaction, add 200 L of LH reagent to the


sample. After adequate reaction, place the prepared sample at the bottom end of
the flow cell. The sample will be wrapped by a sheath stream generated by the
large volume syringe of the syringe linkage and be pushed by the small volume
syringe into the flow cell for the measurement.

1
Dilution ratio refers to the Dilution ratio in the whole blood mode.

7-3
Fluidics

HGB Count
Reagents:

Diluent: used for diluting and cleansing


LH lyse: used for lysing red blood cells and combining hemoglobin
Measurement principle: colorimetric method

Measurement parameters: HGB

Dilution ratio: 1:123.8

Function description: The measurement principle of HGB channel is the colorimetric


method, which obtains HGB concentration by comparing the transmitted light intensity
between background and blood.

7.1.2 RBC/PLT Channel


Reagents:

Diluent: used for diluting, cleansing and equal volume processing conductive environment
and cells
Measurement principle: impedance method

Measurement parameters: RBC and PLT

Graphics: RBC histogram and PLT histogram

Dilution ratio: 1:7533.3

Counting duration: 9 s

Counting pressure: -30kpa

Measurement volume: The measurement volume is controlled by controlling


the vacuum and counting duration. Keep a stable vacuum to ensure a stable flow
out of the aperture. The measurement volume can be calculated by controlling the
counting duration

Function description: Aspirate 21.6 L of sample (dilution ratio 1:69.75) with


the sample probe from the WBC bath. Move the probe to the RBC bath and mix this
sample with 2.4 mL of diluent to prepare a sample with dilution ratio of 1:7533.3.
After mixing, aspirate the sample with negative pressure in the vacuum chamber
through the aperture into the secondary bath. The cells will be measured while
passing through the aperture.

7.2. Sample Volume


Table 7-1 Sample volume

Item Whole Capillary Prediluted Mode

7-4
Fluidics

Blood Blood Mode


Mode
Dilution outside the analyzer: 20L of blood
CBC+DIFF 15L 15L
sample; 480 L of diluent, 200L aspirated
Dilution outside the analyzer: 20L of blood
CBC 11.7L 11.7L
sample; 480 L of diluent, 200L aspirated

7.3. Temperature of Fluidics


Table 7-2 Temperature of Fluidics

Item preheat bath Optical system Diluent


Target Varies with the
Temperature/ diluent / /
temperature
target
Alarm
temperature -1.5,
Temperature/ 40 10, 40
target

temperature +3

7.4. Reagent Consumption Volume


Table 7-3 3107 reagent volume

Diluent DIFF Lyse (ml) LH Lyse (ml) Probe cleanser


Sample Mode
(ml) (ml)
CD 27.5 1 0.2 0
Whole Blood Mode
CBC 23.2 0 0.2 0
CD 27.5 1 0.2 0
Capillary Blood Mode
CBC 23.2 0 0.2 0
CD 27.6 1 0.2 0
Prediluted Mode
CBC 22.9 0 0.2 0
Shutdown 60.9 1 0.2 2
Normal Startup 65 1 0.2 0
Exit Standby 1 3.7 0 0 0
Exit Standby 2 16.9 0 0 0
Exit Standby 3 65 3 0.2 0

7-5
Fluidics

7.5. Introduction to Fluidic Parts


A brief introduction to the fluidic parts and their respective functions is provided in this
section. The symbols mentioned below refer to the symbols in the fluidics diagram.

7.5.1 Mindray Valves


Symbol:
2-way valve 3-way valve

Appearance:
2-way valve 3-way valve

Spring pole

Function:
2-way valve: to build up or cut off a passage. When power off, the passage from
the inlet of the valve to outlet is cut off; when power on, the passage is build up.
3-way valve: to switch among passages. When power off, the public end and the
NO (normally open) end are connected; when power on, the public end and the
N.O.(normally open) end are connected.
Note: the operating voltage of Mindray valves is 12V, and maximal bearable pressure
is 200KPa. The internal movement of the valves is driven by electromagnet and the
restoration is driven by the spring, so it is recommended not put the valves power-on
for too long. When the electromagnet valve is working, the spring pole will lower
down, and it will rise to the initial position when power off. You can touch the spring
pole and feel the descending or ascending, in order to determine whether it is in
action.

7.5.2 2-way Mindray Pressure-proof Valve


Symbol
Same as the 2-way Mindray valve.
Appearance

7-6
Fluidics

Function: Compared with regular 2-way Mindray valve, the 2-way Mindray
pressure-proof valve can endure higher reverse pressure. The operation principle of
the 2-way Mindray pressure-proof valve is the same with that of regular 2-way
Mindray valve.
Note: When replacing the valves, please note the distinction between regular 2-way
valves and pressure-proof valves. SV03 in Liquid Flow Diagram is a 2-way
pressure-proof valve.

7.5.3 LVM Fluidic Valve


Symbol
Same as the Mindray valves.
Appearance
3-way LVM fluidic valve

Function: Same as the Mindray valves. Compared with 2-way Mindray valves, this
valve provides higher pressure resistance and smaller pump volume for preciser flow
control and broader range of temperature and pressure.
Note: the maximal bearable pressure of the LVM fluidic valve is 200KPa, and the CV of
the flow is about 0.03. SV11 in the fluidics diagram is a LVM fluidic valve.

7.5.4 Pinch Valve


Symbol:

7-7
Fluidics

Appearance:

Function: A clamp-on type valve switched by electromagnetic force. Used for


switching the fluid flow.

7.5.5 Liquid Filter


Symbol:

Appearance:
LF

7-8
Fluidics

Function: Used for filtering the impurities in the diluent.

7.5.6 Syringe Linkage


Symbol: SR

Appearance: N/A
Function: Composed of a large volume syringe and a small volume syringe, the syringe
linkage is driven by a motor and a linkage. The parameter and the function of the
syringe linkage are shown in the table below:

Table 7-4 Parameter and function list of the syringe linkage

Specific
Name Function
ation
Used for quantitative aspiration, distribution
Small Full
and secondary aspiration of blood samples, and for
volume scale
injecting the sample into the flow cell for
syringe 250L
measurement
Used for quantitative addition of diluent to

Large Full WBC and RBC bath, wipe fluid supply, cleaning
volume scale of interior and exterior of sample probe and
syringe 10mL reaction bath, forcing the sheath into the flow cell,
cleaning of the flow cell and sample preparation.

7.5.7 Electromagnetic Metering Pump


Symbol

7-9
Fluidics

DP1, DP2
Appearance:

Function:
DP1: 1mL volume. Used for addition of DIFF reagent

DP2: 200L volume. Used for addition of LH reagent

7.5.8 preheat bath


Symbol

preheat Bath
Appearance:

7-10
Fluidics

Function:
Used for heating DIFF reagents to ensure the temperature of DIFF reaction.

7.5.9 Vacuum Pump


Symbol

Appearance:

Function:
LP: Used for draining the wipe, WBC bath, RBC bath and vacuum chamber,
and for creating vacuum in the vacuum chamber

7-11
Fluidics

7.5.10 Sample Probe


Symbol

Appearance:
Open-vial sample probe

Function: Used for providing a rigid cavity with resistance to blood sample corrosion,
which can sample and dispense the blood as well as aspirate and dispense probe
cleanser.
Note: the sample probe is flat-tipped with a side opening to ensure normal aspiration in case that the
tip touches the bottom of the sample tube.

7.5.11 Probe Wipes


Symbol:

7-12
Fluidics

Appearance:

Open-vial probe wipe


Function: Provide a cavity for cleaning open-vial probe or piercing probe by liquid flow
and collecting waste fluids on the interior or exterior.

7.5.12 Hydraulic pressure sensor


Symbol:

Appearance:

Function: Used for monitoring the fluid pressure. When the pressure is obviously
abnormal or beyond the setting range, the sensor will send an alarm signal.

7-13
Fluidics

7.5.13 1-way Valve


Symbol:

Appearance:

Function: Used for controlling the flow direction of the DIFF tube to prevent reverse
aspiration

7.5.14 Baths
WBC bath: Used for providing a place for WBC sample reactions and
supplying well reacted DIFF and BASO samples, and for HGB measurement.

RBC bath: Composed of primary bath, secondary bath and aperture. Used for
providing a place for RBC sample reactions and for RBC/PLT measurement.

Vacuum chamber: Used for creating and keeping a stable negative pressure
for RBC impedance count.

preheat bath: Used for heating DIFF reagents to ensure the temperature of
DIFF reaction.

WBC isolation chamber: Provide a gas chamber to block interference signals


from outside

RBC isolation chamber: Provide a gas chamber to block interference signals


from outside

7.6. Detailed Introduction of Fluidic Structure


The fluidic structure diagram is shown as follows:
Please refer to Appendix A.

7-14
Fluidics

7.6.1 Sampling and dispensing channel


The structure of sampling and dispensing channel is shown below:

Main function:
1. Aspirate and dispense samples. Aspirate 15 L of blood sample by conjunctive use of
small volume syringe of the syringe linkage (SR) and the sample probe (SPB), and
dispense the blood sample.
2. Clean the interior and exterior of the sample probe. The interior is cleaned by the
collaboration of the large volume syringe of the syringe linkage and SV03 and 11 valves.
The exterior is cleaned by the diluent which is forced by the large volume syringe
through SV08, SV07, secondary RBC bath and SV09 into the wipe, with waste fluid
recovered by probe wipe, vacuum chamber and waste pump.
3. Aspirate and dispense probe cleanser. During the aspiration, the SV03 is energized.
2mL of probe cleanser will be aspirated by the SR from the SPB and stored mainly in the
cleanser tanks (T7 and T8). During the dispensation, the SPB is transported by the
sampling assembly to the RBC bath and WBC bath, and the SV03 is kept energized. A
certain volume of probe cleanser in the SPB will be dispensed by the SR to the reaction
baths.

7.6.2 WBC&HGB Channel


Part of the fluidic structure is shown as follows:
The blue lines are diluent flows; red lines are DIFF lyse flows; green lines are LH lyse flows;
orange lines are sample flows; purple lines are waste fluid flows. Similarly hereinafter.

7-15
Fluidics

DIFF measurement
As the background solution, the diluent flows through the large volume syring, SV03, SV11,
PV18 into the WBC bath. After the blood sample is dispensed by the sample probe to the WBC
bath, the DIFF lyse is added by the electromagnetic metering pump (DP1) through the SV01 and
the preheat bath. The diluent is forced by the SR through SV06, T13 and T14 into the isolation
chamber 1, generating bubbles which mix the sample fluid in the WBC bath. The sample is then
supplied along the illustrated orange lines to the bottom end of the flow cell. A sheath flow is
generated by the large volume syringe along the illustrated blue lines, and the sample is forced
by the small volume syringe into the flow cell for measurement to obtain the differential results
of the white blood cells. After the measurement, use the sheath to clean the flow cell and the
sample probe. Force the diluent through the SV03, SV11, C11, T35, SV14 and T36 into the
isolation chamber 2 to clean the sample supply tube.

BASO measurement & WBC count


After DIFF sample enters the sample supply tube, the LH lyse is added by the
electromagnetic metering pump DP2 through SV02 to the WBC bath. The diluent is forced by
the SR through SV06, T13 and T14 into the isolation chamber 1, generating bubbles which mix
the sample fluid in the WBC bath. After the DIFF sample measurement is completed and the
sample supply tube is cleaned, the BASO sample is supplied along the illustrated orange lines to
the bottom end of the flow cell. A sheath flow is generated by the large volume syringe along
the illustrated blue lines, and the sample is forced by the small volume syringe into the flow cell

7-16
Fluidics

for measurement to obtain BASO differential result and WBC count. After the measurement, use
the sheath to clean the flow cell and the sample probe. Force the diluent through the SV03,
SV11 and PV18 into the WBC bath to clean the sample supply tube and the WBC bath. During
the cleaning process, part of the diluent flow away through C11, T35, SV14 and T36 to clean the
3-way connector C11. Finally, the WBC bath is drained by SV15, vacuum chamber (VC) and
waste pump.

HGB Count
The measurement principle of HGB channel is the colorimetric method, which obtains HGB
concentration by comparing the transmitted light intensity between background and blood. The
transmitted light intensity of the pure diluent in the WBC bath is measured at the beginning of
the count. The transmitted light intensity of the diluent with blood is measured after the BASO
reaction is completed (before preparation of the BASO sample). The HGB value can be
calculated by comparing the above two values.

7.6.3 RBC/PLT Channel


Part of the fluidic structure is shown as follows:

7-17
Fluidics

The diluent is added by the SR along the blue line (T3, T18, SV05, T21) to the RBC
bath. The diluted blood sample is dispensed by the sample probe to the RBC bath, and
then is mixed by the bubbles generated when waste cleaning fluid (for cleaning the
DIFF sample fluid in the sample supply tube) enters the isolation chamber 2 through
SV14 and T36. The sample is aspirated by the negative pressure in the vacuum
chamber into the secondary bath (orange lines in the illustration). The cells will be
measured while passing through the aperture. The sample volume is calculated from
the count duration. After the measurement, the RBC bath is drained by the waste
pump, the vacuum chamber and the SV13. In order to clean the secondary bath, the
diluent is forced by the syringe through SV07, T51 and T52 into the secondary bath,
then through T43 and SV09 into the probe wipe, and drained by the waste pump.

7.6.4 Precautions for Assembly and Service


Precautions for installation of sampling assembly
No. Precautions
Strap the tube above the sample probe at the positioning hole to prevent the
1 tube connector from being stressed by the vertical motion of the sample probe
Move the sampling assembly horizontally and vertically to ensure that the
sample tube is unobstructed with no folding and interference with the preamplifier,
2 fluidics separator, right door, motor, valves and tube straps
3 Ensure the sample tube is not squeezed or deformed at the tube straps
Ensure there is no folding or interference when the wipe fluid tube is moving
4 horizontally or vertically in the sampling assembly
When the sample probe is in the top position, keep a distance of 50.1mm
5 between the probe tip and the lower end of the wipe

Precautions for installation of reaction bath assembly


No. Precautions
1 Case T59 and T60 with flexible tubes
The waste tube for the reaction bath needs to be wrapped in the vertical direction
2 to a height above the liquid level with 3mL of liquid in the bath
3 Install the aperture so that the surface with the tapered bore faces the primary bath

Precautions for Servicing the Whole Fluidics


No. Precautions
1 The tubes shall be unobstructed with no twisting, squeezing, creases and folds
Never bend any of the tubes. Pay special attention to the Teflon tubes. If any Teflon
tube is folded, be sure to replace it with a new tube. When a change in direction is
2 needed, use a large arc for transition.
When cutting the tubes, the cutting face shall be perpendicular to the axis of the
3 tube.

7-18
Fluidics

When connecting the adapter with the Teflon tube, make sure the insertion depth is
13~15mm. Keep no clearance between the two connected rigid tubes as far as
4 possible. Keep the end of the Teflon tubes smooth and unwrinkled.
Strap the connections between any 1.0 Teflon tubes and the adapter. Strap at a
5 position near the end of the Teflon tube, and leave 3-5 mm of Telfon tube aside.
When connecting hoses with connectors, valve ports or dosing tubes on the
6 reaction bath, the end of the hose shall be fully inserted beyond the barbs.
7 It is not necessary to over tighten the straps for fastening the tubes.
For T-adapters, the side ports and the middle port shall be treated differently. Please
8 assemble in strictly accordance with the illustration.
There are strict tolerance requirements for lengths of the following tubes: T4 and
T33: 1 mm, T63: 2 mm T7, T8: 3 mm.
9 Tolerances for the other tubes: for length less than 50 mm, the tolerance is 1 mm;
for length between 50 and 400 mm, the tolerance is 2 mm; for length more than
400 mm, the tolerance is 5 mm.
When connecting or replacing tubes on the analyzer, never use any blades or other
10 sharp tools.
11 Never scald any hose with hot water.
Before or after assembly or maintenance, keep all the hoses, connectors or fluidic
components intact and in good condition without any scratches, deformation or
12 distortion.
Thick 50 tubes can not be used again after disconnected from valve ports or
13 connectors
14 Cut T75 and case it between T34 and C11
15 Assemble the check valves so that the bigger end is connected to the preheat bath
Assemble the filters in the right direction so that the words 43 m is shown from
16 top to bottom

7.7. Introduction to Sequences


Taking the WB-CBC+DIFF mode as an example:

7.7.1 Measurement sequence in WB-CBC+DIFF mode


Measurement time in WB-CBC+DIFF mode adds up to 60 s.
The sequence is as follows:

7-19
Fluidics

0~0.9 s The small volume syringe of the SR syringe linkage aspirate 15 L of blood
sample from the sample probe
The large volume syringe aspirates 600 l of diluent from the diluent tank
0.1 s Measure the HGB blank voltage
0.3~2.8 s The liquid pump (LP) is turned on to drain the WBC bath
1~2.7 s Aspirate 5360 l of diluent with the SR (with SV03 and PV18 energized and
SV11 open to T64)
0.1~3.1s Burn the aperture on the RBC bath

7-20
Fluidics

2.8~3.8 s The sample probe travels up to the wipe position


4.2~5.5 s The sampling assembly swings to the position above the WBC bath
2.9~4.2s Use the SR to clean the interior and exterior of the sample probe, and keep
the LP pumping the waste fluid during the whole process
4.2~5.3 s The WBC bath is drained for the second time

7-21
Fluidics

5.5~6 s SR injects 240 L of background solution to the WBC bath


5.2~6 s The sample probe travels down into the WBC bath
6.1~6.5 s The small volume syringe of the SR slowly injects 18 L of blood through the
interior of the sample probe The large volume syringe injects 720 l of fluid to clean the inlet of
the RBC bath
6.55~7.4 s The syringe quickly injects 312 l of fluid to clean the sample probe
(with SV03 energized)
5.3~7.3 s The RBC bath is drained

7-22
Fluidics

7.5~8 s Inject the diluent through SV06 into the isolation chamber 1 to generate
bubbles which mix the sample fluid in the WBC bath
7.4~8.1 s The sample probe travels up to the wipe position and the LP draws waste
fluid from the wipe
8.1~8.4 s The interior of the sample probe is cleaned and the LP draws waste fluid
from the wipe (to 9.2 s)
8.5~9.1 s The syringe aspirates 1580 l of diluent from the diluent tank
8.8~9.6 s The sample probe travels down into the WBC bath
8.6~10.6 s DP1 aspirates the DIFF reagents

7-23
Fluidics

9.6~10.3 s The small syringe aspirates 12 l of diluted sample through the probe
from the WBC bath; the large syringe aspirates 480 l of diluent from the diluent tank
10.3~11.3 s The sample probe travels up to the wipe position; the exterior of the probe is
cleaned by the wipe; the LP draws waste fluid from the wipe
10.6~11.6 s Use DP1 to add DIFF reagent to the WBC bath
11.4~12.3 s Inject the diluent through SV06 into the isolation chamber 1 to generate
bubbles which mix the sample fluid in the WBC bath
11.2~11.9 s The sampling assembly swings to the position above the RBC bath
9.2~10.1 s ,11.4~12.1 s The RBC bath is drained.

7-24
Fluidics

12.4~13.3 s SR injects 1600 L of background solution through SV05 to the RBC bath.
12.3~13.2 s The sample probe travels down into the RBC bath
13.4~14.9 s The sample probe dispenses the blood and clean the interior with 800 L of
fluid
15.4~16.6 s The sample probe travels up to the wipe position and the LP draws waste
fluid from the wipe

7-25
Fluidics

SV08 C17 LF C22 C23


T19 T76 T20

C14 DILUENT
T25 T49
CV1
J7-T31-
T21 J29 T30
J8
SV05 C15
DP1 T50
Preheat SV01
Bath T32

T26

T24
C27
J14-T48-J15-T80-J32 T47
T17

T77
SV02 DP2

J30-T78-
T18

Case

J31

LH Reagent Bottle
T79 SV07 C18
C19

T29

T71
J9
SV04
WBC RBC

T51
PV18
J25-T23-J26

J20-T63- C11 C10


J21 T34 T52
Case T33 C6
Temperature

T72
T75
J22-T64-J23-T65-

J12-T45-

T54
J10-T35-

Sensor
J13

J16-T59-

J18-T60-
J11

J17

J19
C20
J24

SV12

Isolation Chamber

Isolation Chamber
C21
T3

SV16 SV14 C12 C13

DIFF Reagent
T61 T62

T70
1

Bottle
SV06

2
C5 SV13
T9

C4 T12 T13 T14 T37 T38


T39

T40

T55
C9
T15

T36
C3 SV11
T11 T16 C7
J5-T7-J6- SV15

T41
C26
T10

Hydraulic
T53 T8
Sensor
C2 T46 C16
J4-T6 C8
Transducer
J3-T5
T2

T57 T58

T56
T42
J27-T22-J28

SV10 T67
C1 SV03
T43 T66
T1

SPB
J1-T4-

Diluent Tank
J2

T73
VC
T74

SV09

T44
WASTE LP
Waste
Tank C25 C24
T69 T68

SR

15~17.3 s The syringe aspirates the fluid


17.7~18.8 s Aspirate 380 L of DIFF sample fluid from the WBC bath into the sample
supply tube (T63 and T65)
19.2~19.8 s Aspirate 200 L of diluent through C11, T35 and SV14 into T63; adjust the
position of the DIFF sample fluid in the sample supply tube
18.1~19.5 s DP2 aspirates the LH reagent
17~18 s The sampling assembly swings to the top sampling position

7-26
Fluidics

19.5~20.5 s Use DP2 to add LH reagent to the WBC bath


19.8~20.5 s Inject the diluent through SV06 into the isolation chamber 1 to generate
bubbles which mix the sample fluid in the WBC bath
20.5~31.6s The large syringe injects the diluent through SV04 into the flow cell to form
the sheath
20.5~20.7 s SV03 ON; the sample in T65 enters the flow cell quickly by the help of the large
syringe
20.7~31.6 s SV03 OFF; the small syringe injects the sample from the supply tube into the flow
cell to form a stable sample stream
22.2~31.3 s DIFF measurement
31.2 s Measure the HGB voltage

7-27
Fluidics

SV08 C17 LF C22 C23


T19 T76 T20

C14 DILUENT
T25 T49
CV1
T21 J29 J7-T31-J8 T30
SV05 C15
DP1 T50
Preheat SV01
Bath T32

T26

T24
C27
J14-T48-J15-T80-J32 T47
T17

T77

J30-T78-J31
SV02 DP2
T18

Case
T79

LH Reagent Bottle
SV07 C18
C19

T29

T71
J9
SV04
WBC RBC

T51
PV18
J25-T23-J26

C11 C10
J20-T63-J21 T34 T52
Case T33
J22-T64-J23-T65-J24

C6
J12-T45-J13

Temperature
J10-T35-J11

T72
T75

J16-T59-J17

J18-T60-J19

T54
Sensor

Isolation Chamber

Isolation Chamber
C20
SV12
C21
T3

SV16 SV14 C12 C13

DIFF Reagent
T61 T62

T70
Bottle
SV06
C5 SV13
T9

C4 T12 T13 T14 T37 T38


T39
C9

T40

T55
T15

T36
C3 SV11
T11 T16 C7
SV15

T41
Hydraulic C26 J5-T7-J6-T8
T10

Sensor T53
C2 T46 C16
J4-T6 C8
Transducer
J3-T5
T2

T57 T58

T56
T42
J27-T22-J28

SV10 T67
C1 SV03
T43 T66
T1

SPB
J1-T4-J2

Diluent Tank
T73
VC
T74

SV09

T44
WASTE LP
Waste
Tank C25 C24
T69 T68

SR

31.7~33.6 s The large volume syringe aspirates the diluent from the diluent tank ; the
small syringe aspirates 156.3 l of sample fluid from the WBC bath
34~35.5 s Clean the sample supply tube with SV11 and SV14; clean the flow cell with
SV04 and SV16; inject the waste fluid into the isolation chamber 2 to generate bubbles for
mixing the sample in the RBC bath
31.5~39.1 s Create negative pressure in the vacuum chamber

7-28
Fluidics

SV08 C17 LF C22 C23


T19 T76 T20

C14 DILUENT
T25 T49
CV1
J7-T31-
T21 J29 T30
J8
SV05 C15
DP1 T50
Preheat SV01
Bath T32

C27
J14-T48-J15-T80-J32 T47

SV02 DP2
Case
T79 SV07 C18
C19

SV04
WBC RBC
PV18
J25-T23-J26

J20-T63- C11 C10


J21 T34 T52
Case T33 C6
Temperature
T75
Sensor

C20
SV12
C21
SV16 SV14 C12 C13
T61 T62
SV06
C5 SV13
C4 T12 T13 T14 T37 T38
T39
C9
T36
C3 SV11
T11 T16 C7
J5-T7-J6- SV15
Hydraulic C26
T53 T8
Sensor
C2 T46 C16
J4-T6 C8
Transducer
T57 T58
SV10 T67
C1 SV03
T43 T66

SPB

VC

SV09

T44
WASTE LP
Waste
Tank C25 C24
T69 T68

SR

35.8~37.1 s Aspirate 800 L of BASO sample fluid from the WBC bath into the sample
supply tube (T63 and T65)
37.4~51.2 s The large syringe injects the diluent through SV04 into the flow cell to form
the sheath
37.4~37.6s SV03 ON; the sample in T65 enters the flow cell quickly by the help of the
large syringe
37.6~51.2 s SV03 OFF; the small syringe injects the sample from the supply tube into the
flow cell to form a stable sample stream
39.6~51.1 s Perform BASO measurement and WBC count
35 s The constant current source of RBC bath turns ON
35 s~49 s SV12 valve ON; the fluid in the primary RBC bath is forced by the vacuum
through the aperture into the secondary bath
39.8~48.8 s Perform RBC and PLT counts

7-29
Fluidics

51.3~52 s PV18 stays ON; the sample supply tube and the 3-way connector C11 is
cleaned by SV03, SV11, and SV14 Clean the flow cell and the sample probe by SV04
52.1~53.8 s The syringe aspirates the fluid
51.1~52.9 s The RBC bath is drained

7-30
Fluidics

SV08 C17 LF C22 C23


T19 T76 T20

C14 DILUENT
T25 T49
CV1
T21 J29 J7-T31-J8 T30
SV05 C15
DP1 T50
Preheat SV01
Bath T32

T26

T24
C27
J14-T48-J15-T80-J32 T47
T17

T77

J30-T78-J31
SV02 DP2
T18

Case

LH Reagent Bottle
T79 SV07 C18
C19

T29

T71
J9
SV04
WBC RBC

T51
PV18
J25-T23-J26

C11 C10
J20-T63-J21 T34 T52
Case T33 C6
J22-T64-J23-T65-J24

J12-T45-J13

Temperature
J10-T35-J11

T72
T75

J16-T59-J17

J18-T60-J19

T54
Sensor

C20

Isolation Chamber

Isolation Chamber
SV12
C21
T3

SV16 SV14 C12

DIFF Reagent
C13
T61 T62

T70
Bottle
SV06
C5 SV13
T9

C4 T12 T13 T14 T37 T38


T39
C9

T40

T55
T15

T36
C3 SV11
T11 T16 C7
SV15

T41
Hydraulic C26 J5-T7-J6-T8
T10

Sensor T53
C2 T46 C16
J4-T6 C8
Transducer
J3-T5
T2

T57 T58

T56
T42
J27-T22-J28

SV10 T67
C1 SV03
T43 T66
T1

SPB
J1-T4-J2

Diluent Tank
T73
VC
T74

SV09

T44
WASTE LP
Waste
Tank C25 C24
T69 T68

SR

52.9~54.2 s The WBC bath is drained


53.8~54.3 s SV14 ON; C11 is flushed
53.9~54.2 s SV04 ON, the sample probe is flushed
54.1~55.7 s PV18 OFF; the pneumatic pinch tube and the WBC bath are flushed
55.5~56.9 s The WBC bath is drained

7-31
Fluidics

55.8~56.9 s Add 2560 L of diluent to the RBC bath with syringe


57~58.9 s Add 2018 L of diluent to the WBC bath with syringe
59.1~60 s The sample probe travels to the sampling position
59.1~59.4 s Use the small syringe to create a 4 L isolation bubble in the sample probe
58.3~59.4 s The liquid pump (LP) is turned on to drain the wipe
59.4~60 s SV10 ON; the vacuum is released

7.7.2 Measurement sequence in PD-CBC+DIFF mode


Measurement sequence in PD-CBC+DIFF mode is basically the same with the sequence in
WB-CBC+DIFF mode, except that:
1. Because the blood sample has been prediluted outside the analyzer, 200 L of blood is
aspirated in the PD mode.

7-32
Fluidics

7.7.3 Measurement sequence in CBC mode


Compared with the sequence in CBC+DIFF mode, the measurement sequence in CBC mode
is basically the same except that it does not include the fluidic actions of DIFF and optical
channels. The main differences are as follows:
1. Sample volume. The sample volume is 15 L in the WB-CBC+DIFF mode, 11.7 L in the
WB-CBC mode. The sample volume is the same between the PD-CBC+DIFF mode and
PD-CBC mode.
2. There is no actions related to DIFF measurement, including addition of DIFF reagent,
preparation of DIFF samples, DIFF measurement, and cleaning process of the tubes and
the flow cell after the DIFF measurement.

7.7.4 Introduction to the Maintenance Sequences


Probe cleanser maintenance (shutdown sequence)
The locations soaked by the probe cleanser are shown below.
The orange lines are the locations soaked by the probe cleanser, and the blue lines are the
locations passed by the probe cleanser.
An enhanced probe cleanser maintenance sequence will be called every 300 times of
sample measurement. The main difference between the enhanced and the regular probe
cleanser maintenance sequence is the soaking time. The soaking time of enhanced maintenance
is 6.67 minutes longer than regular maintenance.
In the Maintenance screen, the probe cleanser maintenance is defaulted as enhanced probe
cleanser maintenance.

7-33
Fluidics

T26

T24
T17

T77

J30-T78-J31
T18

LH Reagent Bottle
T29

T71
J9

T51
J22-T64-J23-T65-J24

J12-T45-J13

J10-T35-J11

T72
T54
J16-T59-J17

J18-T60-J19
Isolation Chamber

Isolation Chamber
T3

DIFF Reagent
1

T70
Bottle
T9

T40

T55
T15

T41
T10

J3-T5

T56
T42
J27-T22-J28

Diluent Tank
J1-T4-J2

T73
T74

Startup cleaning
The fluidic actions on startup can be divided into three procedures:
1. Initialization of fluidics components: initialization of sampling assembly and syringe
assembly; 1 metering pump action (without consumption of reagents); creating vacuum and
releasing vacuum.
2. Cleanup: including cleaning of all the tubes and components on the analyzer; removing
bubbles from the flow cell; discarding 1 ml of DIFF reagent and 0.2 ml of LH reagent.
3. Background: WB-CBC+DIFF measurement
If the background fails, perform the cleanup again and measure the background.
For startup after an abnormal shutdown, perform the cleanup twice.

Standby
The instrument will enter standby status after idling for 15 to 30 minutes (defaulted as 15
minutes). After entering standby status, operations without fluidic actions can be performed
from the screen.
Exit standby status 1: Standby for less than 1 hour.
Clean the exterior of the sample probe and the WBC bath and rebuild the

7-34
Fluidics

isolation bubble without consumption of lyse.


Exit standby status 2: Standby for more than 1 hour and less than 5 hour.
Clean the interior and exterior of the sample probe, the WBC bath, the RBC bath, the
sample supply tube and the flow cell, and rebuild the isolation
bubble without consumption of lyse.
Exit standby status 3: Standby for not less than 5 hour.
The same as cleanup on startup, including cleaning of all the tubes and components on the
analyzer; removing bubbles from the flow cell; discarding 3 ml of DIFF reagent to eliminate the
effects of bubbles in the preheat bath tube; discarding 0.2 ml of LH reagent to eliminate the
effects of crystal and bubbles at the inlet.

7-35
8 Hardware System

8.1. Hardware System Overview


The hardware system consists of not only power board, main control board, indicator board and
analog drive board for touchscreen, but also drivers and components that requires power supply, such
as motors, valves, pumps, sensors, display and input power filter, and connecting wires between
different boards and components.

8.1.1 Functional Block Diagram


The functional block diagram of the hardware system is shown in Figure 8-1.

Data Channel Master Control


Sample review Touch screen
Signal Signal
Sensor
preprocess sampling Result display TFT monitor
Signal
preprocess Result print USB printer

Channel Control Data storage

Data manager Network


interface
Bar-code
Input module
scanner
Drive/Detect
Control Start key
Optical
Valve and Motor Alarm
switch
pump control control control
control

POWER Supervisor
supervise system Indicator
POWER board
State
supervise

Figure 8-1 Functional block diagram of the hardware system

The hardware system consists of five modules, including power supply, data stream channel,
control system, drive components and peripherals. The functions of each modules are as follows:

1. Power supply: provides all kinds of power for each board, component and devices in the
hardware system;
2. Data stream channel: for extraction, modulation, amplification, collection and preprocessing
of signals;
3. Control system: for data collection, data processing, result display and sample storage. The

8-1
Hardware System

control system is also the scheduling and managing center, which controls and responds to
all the peripherals and devices;
4. Drive/monitor: for controlling valves, pumps and motors, monitoring photocoupler and other
important parameters, collecting measurement data and triggering alarms;
5. Peripheral interface: includes display/touchscreen, USB ports (for printer, keyboard and
barcode scanner) and Ethernet interface. Peripherals also includes operation indicators and
key inputs.

8.1.2 Electrical Connection Block Diagram


The diagram below is the hardware connection block diagram. For electrical connection diagram
of the optical system, please refer to the optical system hardware section.

Figure 8-2 Electrical connection block diagram of the hardware system


The detailed electrical connection diagram of the hardware system is shown in Appendix C.

8.1.3 System Troubleshooting


Common hardware system failures can be divided into board failures, wire failures and
component failures. Generally, the troubleshooting procedures of these failures can be found in the
board troubleshooting section below. However, when the system power supply can not be guaranteed

8-2
Hardware System

(such as failure to power up or immediate system self-protection after power-up), it will be necessary to
start troubleshooting from the system level. Figure 8-3 shown the flowchart for power anomaly check.
Figure 8-4 shows the filter which is located below the rear of the analyzer, used for controlling power
supply and frequency filtering.

Figure 8-3 Abnomal power troubleshooting flowchart

Figure 8-4 Actual picture of the filter


Figure 8-5 shows the troubleshooting flowchart for immediate self-protection after
power-up.

8-3
Hardware System

Figure 8-5 Troubleshooting flowchart for power-up protection

8.2. Digital Control Board

8.2.1 Overview
The digital control board consists mainly of a digital part and an analog part. The analog part
implements mainly the A/D conversion of analog signals into digital signals, such as optical channel
and monitoring voltage. As the essential part of the digital control board and the whole hardware
system, the digital part implements data processing, output, control and communication.
Section 8.2 of this manual is the guideline for service and troubleshooting of the control board.

8.2.2 Components
The block diagram of the control board is shown in Figure 8-6. The control board consists mainly
of digital circuit, plus part of the ADC circuit which implements the A/D conversion. The digital circuit
module implements data processing, result saving and output. As the essential part of the digital control
board and the whole hardware system, the digital circuit is also responsible for control and
communication. The ADC circuit is mainly responsible for digitizing WBC, RBC/PLT, optical signals
and all kinds of analog monitoring signals with an A/D converter.
The control part of the control board employs the CPU+FPGA structure to achieve the
following main functions:

A/D conversion
Data processing
Peripheral interface implementation
Control interface extension

8-4
Hardware System

Figure 8-6 Block diagram of the control board

Description

A/D conversion
Converts the analog signals to digital signals which can be processed by FPGA or CPU.

Data processing
The digital signals obtained by FPGA from A/D samples are digitally filtered to save
the particle parameters. The data then is transmitted to CPU by interruption or other methods
for further processing. After processing, the results will be shown on the LCD.

Peripheral interface implementation


The CPU module provides a platform for system software, interfaces for peripherals
including indicator board, LCD, Ethernet, USB printer, USB barcode scanner, keyboard and
USB drive, etc., the JTAG interface for online programming the FPGA configuration chip,
and the CPU debug interface.

Control interface extension


Provides control logic and interface for LCD, SD card and touchscreen, etc.

8-5
Hardware System

Figure 8-7 Schematic of the digital control board module


Interface definition
The functions of the 13 socket interfaces on the control board is listed in Table 8-1. The locations
of each interface on the board is shown in Figure 8-8.

J78 Indicator J99 Start KEY J81 Communication J68 POWER


interface

J86 supervise
signal interaface

J77 control for


Analog&driver
board and optical J2 USB
system
J3 USB

J1 Network

J85 Optical
signal input
interface

J16 TFT Monitor J4 TFT Monitor J67 Touch


Backlight Screen

8-6
Hardware System

Figure 8-8 Interface locations of the digital control board


Table 8-1 Function list of the control board interfaces

Interface Function PIN Function Description Description


J1 Ethernet interface / Network interface
J2 USB port / Connect different
USB peripherals
J3 USB port / Connects different
USB peripherals
J4 LCD backlight PIN1: Backlight power Drives backlight and
control interface supply, 12V control the
PIN3: Backlight power brightness
supply, GND
PIN5: Enables backlight,
3.3V
PIN6: Drives backlight,
0.7~1.2V
J16 LCD signal PIN3: Power supply, 3.3V Provides differential
interface PIN5: GND signal for the LCD to
display data
J67 Touchscreen PIN1: Power supply, 3.3V Connects the
signal interface PIN4: GND touchscreen
J68 Power interface PIN1: Power supply, 5V, Supply power for
GND the boards
PIN2: Power supply, 12V
GND
PIN3: Power supply, 5V
PIN4: Power supply, 12V
J77 Analog board PIN1: GND Analog board
control and optical PIN22: 3.3V control and optical
SPI interface PIN24: 5V SPI interface
J78 Indicator board PIN1: 5V Drives indicator and
interface PIN2: RED_LED buzzer
PIN4: GREEN_LED
PIN6: YELLOW_LED

8-7
Hardware System

PIN9: GND
J81 Analog board and / Analog board and
control board SPI control board SPI
interface communication
interface
J85 Optical signal PIN1: FS Signal Interface for optical
input interface PIN3: SS Signal signal inputs
PIN5: SF Signal amplified by the
PIN7: WBC Signal analog board
PIN9: RBC Signal
PIN11: 5V
PIN12: 5V
PIN13: GND
PIN14: GND
J86 Analog board PIN13: 5V Provides supervisory
supervisory signal PIN20: GND signals for the
interface control board
J99 Aspirate key PIN2: GND Aspirate key switch
switch interface interface

8.2.3 Adjustment and Tests


All the adjustable parameters of this board are adjusted by commands. To adjust the parameters,
perform parameter adjustment in the software interface.
Function definition of LED indicators
Functions of LED indicators on the control board are shown in Table 8-2:
Table 8-2 Function definition of LED indicators on the control board

Indicator Function LED OFF Diagnosis


D6 Network interface indicator Network disconnected, network cable
which flashes after failure, control board failure
connected with network
cable and PC
D7 Network interface indicator Network disconnected, network cable
which is illuminated after failure, control board failure
connected with network
cable and PC

8-8
Hardware System

D40 USB related signals. USB peripheral failure, control board


Illuminated after power-up. failure
D44 power chip indication signal. Power board failure, control board
Illuminated after power-up. failure
Function definition of test points
The function of test points are defined in Table 8-3. The zone code of test points are shown in
Figure 8-7.
In order to exclude problems caused by shorted peripherals, remove all the wires except the power
cable before diagnosing a power supply problem.
Table 8-3 Function definition of test points on the control board

Test
No. Description Zone Diagnosis
Point
1 TP1 VDD18 1.8 V Zone 6 The voltage is not 1.8V: control board failure
voltage monitoring or power board failure
point
2 TP2 VDD 3.3V voltage Zone 6 The voltage is not 3.3V: control board failure
monitoring point or power board failure
3 TP3 VDD12 1.2 V Zone 6 The voltage is not 1.2V: control board failure
voltage monitoring or power board failure
point
4 TP4 Backlight enable Zone 5 The voltage is not 3.3V: control board failure
signal
5 TP5 Backlight PWM Zone 5 The voltage is not 0.7~1.2V: control board
signal failure
6 TP6 Backlight GND Zone 5 /
signal
8 TP9 Analog ground Zone 1 /
9 TP10 ADC U79 SPI Zone 1 /
clock signal
10 TP11 ADC U78 SPI Zone 1 /
clock signal
11 TP12 ADC U79 2.5V Zone 1 The voltage is not 2.5V: control board failure
reference voltage or connection failure between control board
J86 and analog drive board
12 TP13 ADC U78 2.5V Zone 1 The voltage is not 2.5V: control board failure

8-9
Hardware System

reference voltage or connection failure between control board


J86 and analog drive board
13 TP14 FS analog signal Zone 7 /
14 TP15 FS analog GND Zone 7 /
15 TP16 FS AD conversion Zone 7 /
clock
16 TP17 SF analog signal Zone 7 /
17 TP18 SF analog GND Zone 7 /
18 TP19 SF AD conversion Zone 7 /
clock
19 TP20 SS analog signal Zone 7 /
20 TP21 SS analog GND Zone 7 /
21 TP22 SS AD conversion Zone 7 /
clock
22 TP23 RBC AGND Zone 7 /
23 TP24 RBC analog signal Zone 7 /
24 TP25 RBC AD analog Zone 7 /
signal
25 TP26 RBC VREF Zone 7 /
reference voltage
26 TP27 WBC VREF Zone 7 /
reference voltage
27 TP28 WBC AGND Zone 7 /
28 TP29 WBC analog Zone 7 /
signal
29 TP30 WBC AD Zone 7 /
conversion clock
31 TP32 Touchscreen Zone 5 /
interruption signal
32 TP33 Touchscreen reset Zone 5 /
signal
33 TP34 Digital ground Zone 5 /
34 TP35 VDD25 2.5 V Zone 6 The voltage is not 2.5V: control board failure
voltage monitoring or power board failure
point

8-10
Hardware System

35 TP36 5 V voltage Zone 6 The voltage is not 5V: control board failure or
monitoring point power board failure
36 TP37 12V voltage Zone 8 The voltage is not 12V: control board failure
monitoring point or power board failure (the ground connection
of this voltage is J68_PIN2 instead of GND)
37 TP44 DDR2 0.9V Zone 6 The voltage is not 0.9V: control board failure
voltage monitoring or power board failure
point
38 TP45 DDR2 0.9V Zone 6 The voltage is not 0.9V: control board failure
reference voltage or power board failure
monitoring point
39 TP38, Digital ground Zone 6 /
TP39,
TP40,
TP41,
TP42,
TP43,
TP46,
TP47
40 TP48 3.3 V voltage Zone 5 The voltage is not 3.3V: control board failure
monitoring point or power board failure

8.2.4 Troubleshooting
Table 8-4 lists common symptoms and relative corrections for the control board only from the
hardware side, not including symptoms caused by software. However, many problems will need to be
tested by software.
Before troubleshooting problems related to the control board, perform the following checks:

1. whether there is any loose connecting wire or unreliable connection on the control board;
2. whether the bit numbers on the wires are matching the bit numbers on the control board
sockets; whether there is any broken or damaged wire;
3. whether the input power of board socket J68 is working properly (measured with a
multimeter, the voltage between PIN1 and PIN3 shall be 5V, and the voltage between PIN2
and PIN4 shall be 12V);
4. Verify if the indicator on the data board is normal according to Table 8-2.
After the wire connections, input power and indicators are verified to be normal, troubleshoot the
problem in accordance with Table 8-4.

8-11
Hardware System

Table 8-4 Troubleshoot the control board

No. Symptom Evidence Solution


1 LCD black 1. Check if the connecting wires between the Reconnect the
screen control board and the backlight interface and connecting wires
LCD is reliable. If the problem is solved after from the control
reconnection and power-up, then the problem is board to the backlight
caused by unreliable wire connection. Otherwise, and LCD
proceed to the next step.
2. Replace the connecting wires from the Replace the
control board to the backlight and LCD. If the connecting wires
problem is solved, then the problem is caused by from the control
connection failure. Otherwise, proceed to the next board to the backlight
step. and LCD
3. Measure the voltage between PIN1 and Replace the control
PIN3 at J4 with a multimeter. If the measured board
value is not between 11.50~12.50, then the
problem is caused by backlight power failure.
Otherwise, proceed to the next step.
4. Measure the voltage between TP5 and
TP6 with a multimeter. If the measured value is
not between 0.7~1.1V, then the problem is caused
by backlight brightness control failure.
Otherwise, proceed to the next step.
5. Measure the voltage between TP4 and
TP6 with a multimeter. If the measured value is
not between 3.10~3.50V, then the problem is
caused by backlight enable control failure.
6. Replace the LCD. If the problem is Replace the
solved, then the problem is caused by LCD LCD
component failure. Otherwise, proceed to the next
step.
2 LCD 1. Reconnect the connecting wires from the Reconnect the
display control board to the backlight and LCD. If the connecting wires
flickers problem is solved after reconnection and from the control
power-up, then the problem is caused by board to the backlight

8-12
Hardware System

unreliable wire connection. Otherwise, proceed to and LCD


the next step.
2. Replace the connecting wires from the Replace the
control board to the backlight and LCD. If the connecting wires
problem is solved, then the problem is caused by from the control
connection failure. Otherwise, proceed to the next board to the backlight
step. and LCD
3. Replace the control board. If the problem Replace the
is solved, then the problem is caused by control board
differential conversion chip (U22) or AM1808
board failure. Otherwise, proceed to the next step.
4. Replace the LCD (LCD component). If Replace the
the problem is solved, then the problem is caused screen assembly
by LCD component failure.
3 LCD 1. Reconnect the connecting wires from the Reconnect the
displays control board to the LCD. If the problem is connecting wires
strange solved after reconnection and power-up, then the from the control
patterns problem is caused by unreliable wire connection. board to the LCD
Otherwise, proceed to the next step.
2. Replace the connecting wires from the Replace the
control board to the LCD. If the problem is connecting wires
solved, then the problem is caused by connection from the control
failure. Otherwise, proceed to the next step. board to the LCD
4. Replace the control board. If the problem Replace the
is solved, then the problem is caused by control control board
board failure. Otherwise, proceed to the next step.
5. Replace the LCD (LCD component). If Replace the
the problem is solved, then the problem is caused screen assembly
by LCD component failure.
4 Failed 1. Check if the configured IP of the PC is Set the IP
network within the same network segment with the control address to 10.0.0.3
connection board (10.0.0.X). If not, set the IP to 10.0.0.3. If
the problem is solved, then the problem is caused
by incorrect IP address. Otherwise, proceed to the
next step.

8-13
Hardware System

2. D6 and D7 are not illuminated after Reconnect or


power-up and connected with PC, and there is replace the network
poor contact or bad network cable. Otherwise, cable
proceed to the next step.
3. D6 and D7 are not illuminated, but the Replace the
network cable contact is normal. control board
5 USB port 1. If D40 is not illuminated after power-up, Replace the
is not then the USB HUB chip (U56) fails. Otherwise, control board
working proceed to the next step.
properly 2. If D40 is illuminated after power-up, Replace the USB
replace the USB peripherals (USB mouse, USB peripherals
keyboard or USB drive). If the problem is solved,
then the problem is caused by USB peripheral
failure. Otherwise, proceed to the next step.
3. After all these steps, if the problem still Replace the
exists, replace the digital control board. control board

6 Clock 1. Turn off the power and measure the Replace the
reset at voltage between the two end of Battery clip B1 button battery
every with a multimeter with the battery in place. If the
startup measured value is less than 1.8V, then the
problem is caused by a low battery. Otherwise,
proceed to the next step.
2. Measure the voltage of pin 1 or pin 4 at Replace the
X10 with a multimeter. If the measured value is control board
0V, then the problem is caused by crystal
resonator X10.
7 No 1 Check if the connecting wire of the
response aspirate key is loose or broken. If so, reconnect or
when replace the wire.
pressing the 2. If step 1 does not solve the problem,
aspirate key remove the aspirate key switch plate to check if
there is fluid inside. If so, clean the fluid and
reinstall the switch plate.

8-14
Hardware System

8.3. Analog Drive Board

8.3.1 Overview
The functions of analog drive board includes measuring and amplifying the signals from the RBC
channel, the HGB channel and the optical channel, and outputting them to external boards; responding
commands of the control board; controlling mechanical components (such as motor drive assembly)
and fluidics components (such as valves and pumps); detecting component position when controlling
mechanical components (through the photocoupler); detecting pressure when controlling fluidics
components (such as pumps); reporting necessary information (temperature, voltage, etc.) to the control
board.

8.3.2 Components
The analog drive board can be divided by modules into four modules: power module, master
control module, detection module and power driving module. The modules are shown in Figure 8-9.

Analog part Digital part


Temp sensor and Liquid
Analog optical signal pressure Pump and Valve
power amplifier sensor Driver

1 2 3 4

Motor Driver
5
7
11
RBC

12
For Debug
Air pressure

10 8 6 13
sensor

9
Signal Supervise Communication Master
HGB
preprocess signal Module Control

Figure 8-9 Module division of the analog drive board


The analog part modulates and amplifies the WBC, RBC/PLT and HGB signals so that the signals
are basically real and ready for A/D conversion before entering A/D. The A/D module is the interface
between the analog circuit and the digital circuit for sampling the above-mentioned sensor signals and
other supervisory signals and converting analog signals to digital signals for digital circuit.

8-15
Hardware System

1. The power supplies power for all the electronic devices on the analog drive board, including
both input power from the analog drive board, and the power converted by the power chip;
2. The master control module is responsible for communicating with the control board and task
scheduling;
3. The detection module is responsible for collecting information such as temperature, pressure,
voltage and fluid in the tubes;
4. The power driving module is responsible for driving the power components such as motor,
heater, fan, valves and pumps;
5. The RBC detection circuit provides the RBC module with constant current source and functions
such like RBC signal modulation;
6. Provides functions such like modulation of optical signals and HGB signals.

Figure 8-10 Location of connectors on the analog drive board

Table 8-5 Function list of the analog drive board interfaces

Interface Function PIN Description


J1 Float sensor PIN2: GND Detect float status
interface
J2 Analog drive board PIN1: Optical Outputs board status
status signal output temperature information to the
PIN2: Aperture control board
supervisory voltage

8-16
Hardware System

PIN3: Gas pressure


PIN4: AGND
PIN5: Fluid pressure
PIN6: -12V supervise
PIN7: Constant current
source supervise
PIN8: Power 24V
supervise
PIN9: Laser current
supervise
PIN10: Power 12V
supervise
PIN11: Analog 12V
supervise
PIN13: Analog 5V
output
PIN20: AGND
J3 Optical board PIN22: 3.3V, provided Controls the optical
control signal by the control board boards. This signal is
PIN24: 5V provided by only buffered by the
the control board to DPM analog drive board.
PIN23: GND
J4 Optical analog PIN1: FS output Optical signal
signal output PIN3: SS output output modulated by
PIN5: SF output the analog drive
PIN9: RBC output board
PIN14: AGND
J5 HGB bath PIN1: AGND HGB bath interface
interface PIN2: HGB LED+
PIN4: HGB current
input
PIN5: HGB LED-
J6 RBC bath PIN1: Aperture
interface electrode B
PIN2: Aperture

8-17
Hardware System

electrode A
PIN3: AGND
J8 Analog power PIN1: AC120_A Supply analog
interface PIN2: AC120_B power for the boards
PIN4: Analog 12V
PIN5: Analog -12V
PIN3: AGND
PIN6: AGND
J9 Optical system PIN3: FS input Optical board
signal input and PIN7: SS input analog signal output
control output PIN11: SF input and control signal
PIN15: Laser current input
output
PIN18: Laser control
PIN16: AGND
J10 Temperature sensor / 3-way temperature
interface sensor interface
J11 Hydraulic sensor / Hydraulic sensor
interface interface
J12 Photocoupler / Photocoupler
interface for interface for
sampling assembly sampling assembly
J13 Photocoupler / Photocoupler
interface for interface for syringe
syringe assembly assembly
J14 Valve 13-18 driver / Drives valve 13-18
J15 Valve 1-12 driver / Drives valve 1-12
J17, J18, Fan interface / Fan interface
J19
J20 Waste pump / Waste pump
interface interface
J21 Heater interface / Heater interface
J22 Power interface for PIN1: Power 12V Digital power
digital part of the PIN3: Power 5V interface
analog board PIN5: Power 24V

8-18
Hardware System

PIN2: GND
J23 Motor driver / Motor driver
interface interface
J24 Motor driver / Motor driver
interface interface
J31 Controls analog / Controls analog
drive board DPM drive board DPM by
by the control the control board and
board and other other preserved
preserved interfaces
interfaces

8.3.3 Sockets and Indicators


Function definition of LED indicators
Functions of LED indicators on the analog drive board are shown in Table 8-6:
Table 8-6 Function definition of LED indicators on the analog drive board

Indicator Function Diagnosis


D102 Heater status LED OFF indicates the heater is not
indicator working
D104-D109 Motor status indicator LED OFF indicates the motor is not
working
D112 MCU status indicator LED OFF indicates MCU is not
working. May be caused by analog drive
board failure
D115-D117 FPGA controlled LED OFF indicates FPGA is not
LED working. May be caused by analog drive
board failure
D118 Digital 24 V power LED OFF indicates power failure.
indicator Check the power board and the analog
D119 Digital 12V power drive board
indicator
D120 Digital 5V power
indicator
D121 Digital 3.3V power
indicator

8-19
Hardware System

D122 Analog 12V power


indicator
D123 Analog -12V power
indicator
D124 Analog 5V power
indicator
D125 Analog -5V power
indicator
Function definition of test points
Functions of test points on the analog drive board are shown in Table 8-7. The zone codes are
shown in Figure 8-8.
Table 8-7 Function definition of test points on the analog drive board

Test Diagnosis
Description Zone
Point
TP19 Analog 12V If the voltage is not 2.28V, then the
voltage AD Zone 8 problem may be caused by power failure or
monitoring point analog drive board failure
TP21 FS baseline /
voltage AD Zone 8
monitoring point
TP22 Analog -12V If the voltage is not 2.82V, then the
voltage AD Zone 8 problem may be a power failure or analog
monitoring point drive board failure
TP23 56V voltage If the voltage is not between 1.25~1.5V,
monitoring point Zone 8 then the problem may be a power failure or
analog drive board failure
TP26 Hydraulic sensor /
Zone 3
output voltage
TP29 Pressure sensor If the voltage is not 2.5V, then the problem
2.5V voltage Zone 10 may be a power failure or analog drive
monitoring point board failure
TP30 Pressure sensor /
output voltage Zone 12
monitoring point
TP39, Motor lock Zone 5 /

8-20
Hardware System

TP40, voltage
TP49,
TP58
TP59 Reset chip Above Low signal indicates the chip is in reset
output signal Zone 7 status
TP68 Digital ground Zone 6 /
TP43, Motor pulse /
TP44, output Zone 5
TP51 monitoring point
TP69 D115 signal /
Zone 7
monitoring point
TP86 Digital 1.2V If the voltage is not 1.2V, then the problem
power Zone 6 may be a power failure or analog drive
monitoring point board failure
TP87 Digital 2.5V If the voltage is not 2.5V, then the problem
power Zone 13 may be a power failure or analog drive
monitoring point board failure
TP88 Digital 3.3V If the voltage is not 3.3V, then the problem
Right to
power may be a power failure or analog drive
Zone 11
monitoring point board failure
TP89 Digital 12V If the voltage is not 12V, then the problem
Right to
power may be a power failure or analog drive
Zone 4
monitoring point board failure
TP90 Digital 24V If the voltage is not 24V, then the problem
Right to
power may be a power failure or analog drive
Zone 4
monitoring point board failure
TP91 Digital 5V If the voltage is not 5V, then the problem
power Zone 5 may be a power failure or analog drive
monitoring point board failure
TP92 AD U41 /
conversion chip
Zone 3
voltage
monitoring point
TP112 HGB analog /
Zone 9
voltage output

8-21
Hardware System

TP113 HGB assembly /


current to
Zone 9
voltage
monitoring point
TP115 HGB LED 2.5V If the voltage is not 2.5V, then the problem
monitoring point Zone 9 may be a power failure or analog drive
board failure
TP116 Burning voltage /
Zone 12
switch
TP117 Constant current /
Zone 12
source switch
TP119, Laser control /
Zone 12
TP120 switch
TP121 RBC relay /
Zone 12
control signal
TP122 Analog 12V If the voltage is not 12V, then the problem
voltage Zone 1 may be a power failure or analog drive
monitoring point board failure
TP123 AVDD analog If the voltage is not 5V, then the problem
5V voltage Zone 1 may be a power failure or analog drive
monitoring point board failure
TP111, Analog GND Zone 11 /
TP124, shield is
TP125 the analog
ground
TP126 Analog -12V If the voltage is not -12V, then the problem
voltage Zone 1 may be a power failure or analog drive
monitoring point board failure
TP127 AVEE analog If the voltage is not -5V, then the problem
-5V voltage Zone 1 may be a power failure or analog drive
monitoring point board failure
TP128 NE555 U75 /
Zone 1
pulse output
Key definitions
Functions of keys on the analog drive board are shown in Table 8-9:

8-22
Hardware System

Table 8-8 Function definition of keys on the analog drive board

Bit No. Function


S2 Manually reset the
system

8.3.4 Troubleshooting
1) Validation before troubleshooting
Before troubleshooting driver related problems, check whether there is any loose connecting wire
or unreliable connection on the analog drive board, and whether the bit numbers on the wires are
matching the bit numbers on the analog drive board sockets; whether there is any broken or damaged
wire.
When analog drive board failure is suspected, verify whether the indicators on the analog board
are normal in accordance with the table below.
After all the indicators is verified to be normal, verify the MCU and FPGA from the software
interface (click Menu click Status Version information). If all the versions are correct,
troubleshoot the problem according to the problem types listed in (2).
2) Troubleshooting
Initially locate the problem by the alarm indication, then troubleshoot the problem according to
the type as shown in Table 6-7.
Table 8-11 Troubleshooting and solutions

No. Problem Type Description Troubleshooting and solutions


1 Motor and This problem 1. Check if the board power supply is working
photocoupler type includes properly;
but is not 2. Check if the connection between the motor of
limited to: corresponding channel and the photocoupler is
a. the motor reliable, if the connectors on both ends are connected
does not work; properly, if the marks on the photocoupler and motor
b. the motor connecting wires match their respective locations,
works, but and if there is any broken or damaged wire;
motor failure or 3. After verifying 1 and 2, try correcting the problem
photocoupler by performing Remove Error or Self-Test from the
failure is software screen;
reported 4. Check if the photocoupler surface of the
corresponding channel is contaminated by dust or
fluids. If so, clean and reinstall the photocoupler. If

8-23
Hardware System

the problem is not solved, replace this photocoupler;


5. If the problem still exits after replacing the
photocoupler, replace the analog drive board;
6. If the problem persists, replace the corresponding
channel motor;
7. If the problem persists, then the problem may be
caused by mechanical component failure (such like
too much friction), please troubleshoot this problem
as a mechanical problem.
Abnormal 1. Check if the corresponding channel motor
motor noise connecting wire is loose, broken or damaged. If so,
please reconnect or replace the wire with the power
off;
2. Check if any fastening screw of the mechanical
component is loose. If so, please tighten this screw;
3. If both 1 and 2 can be excluded, the problem may
be caused by analog drive board failure. Please
replace the analog drive board;
4. If the problem persists, it will be necessary to
replace the motor assembly.
2 Valves The valve is not 1. From the Valve Self-test screen, check if the valve
working is opening and closing correctly (a clap will be heard
properly on normal open/close of the valve). If so, then the
problem is not in the valve drive. Please search for
the cause in the fluidics;
2. If the valve is not opening and closing correctly,
please check if there is any loose or broken wires or
unreliable connection. If so, please reconnect or
replace the connecting wire;
3. If the problem persists, use wires of other valves to
connect this valve, and check if the problem is in the
valve start circuit or in the valve from the Valve
Self-test screen (for example, if valve 2 is suspected,
use wires of valve 3 to connect valve 2; open and
close valve from the Valve Self-test screen; if the

8-24
Hardware System

valve is opening and closing correctly, then the


analog drive board is damaged and needs to be
replaced; if the valve is not opening and closing
correctly, then the valve 2 is damaged and needs to be
replaced).
3 Pumps a. abnormal 1. Check if the pump is able to work properly from
pressure the Pump Seft-test screen. If it is, search for the
b. the pump problem in the gas circuit;
does not work 2. If the pump is not opening and closing correctly,
please check if there is any loose or broken wires or
unreliable connection. If so, please reconnect or
replace the connecting wire;
3. If the problem persists, replace the analog drive
board;
4. If the problem persists, it will be necessary to
replace the corresponding pump.
4 Communication Communication 1. Check if the control board indicator and the analog
failure reported board indicator are normal. If not, then replace
corresponding board(s);
2. Check if the connecting wire between the control
board and the analog board is loose. If so, then
reconnect the wire;
3. If the problem persists, replace the connecting wire
between the control board and the analog board;
4. If the problem persists, replace the analog drive
board and the control board one after another. Most
problems will be solved in this way.
5 Waste sensor False alarm of 1. Check if the connecting wire on the waste
waste status connector is loose, wet or broken. If so, please
disconnect and reconnect the connector, or reconnect
the connector after cleaning the fluid, or replace the
connecting wire.
2. If not, replace the waste cap assembly.

8-25
Hardware System

8.4. Power Board

8.4.1 Overview
The power board provides the analyzer with 6 reliable power outputs, including D5V, A+12V,
A-12V, AC120V and P24V.
Interface definition
There are 6 interfaces for external connections on the power board. 4 interfaces are in socket form,
namely J1, J2, J3 and J4; the AC input wire L and N are led from the board side to the plug for external
connection; the small inverter board is directly connected to the power board by the interface socket.
The locations of each interface on the board are shown below:

A small inverter board

Figure 8-11 Schematic diagram of the power board


The functions of each interface are listed below:
Table 8-12 AC input wires

PIN Definition
L Connected to utility live wire
N Connected to utility neutral wire

Table 8-13 Output socket connectors

Name Socket No. Description


D5V J1 PIN1: GND
PIN2, PIN3: 5V
P12V, P24V J2 PIN1: GND
PIN2: 12V

8-26
Hardware System

PIN3: 24V
A+12V, A-12V J3 PIN1, PIN4: GND
PIN2, PIN3, PIN5: -12V
PIN6: 12V
AC120V J4 AC

8.4.2 Replacement and Connection


Purpose: The power board is one of the key components in the analyzer. If any board problem
occurs, it is required to replace or repair the board timely to ensure normal operation of the analyzer.
Please use the following procedure to replace the power board.
Tool: A 107 cross-head screwdriver and a multimeter.
Removal: 1. Shutdown the analyzer and disconnect the AC power cable;
2. Remove the power assembly from the main unit;
3. Open the power housing, remove 4 fastening screws on the power board, and remove
the power board from the housing.

Please wear antistatic gloves when removing the board;


Please make sure the power is off and disconnect the power cable before removing
the boards.

Installation:
Reverse the removal procedure.
Verification: 1. Check if all the screws are installed on the board;
2. Connect the power cable and turn on the AC control switch. Now the analyzer will be
initialized, and all the board indicators will be illuminated.

Make sure the power assembly is firmly connected by screws with the main unit.
Only perform these removal procedures after the power board and the surroundings
are cooled down.

8.4.3 Troubleshooting
The troubleshooting procedures for the power board are shown below.

8-27
Hardware System

Figure 8-12 Troubleshooting flowchart of the power board

8.5. Optical Boards

8.5.1 Optical Path and Optical System Workflow

Figure 8-13 shows the schematic diagram of the optical path. The laser is a semiconductor laser
with wavelength of 670 nm. The laser irradiates the cells in the flow cell, which generate scattered light.
This scattered light intensity is measured by photodiode (PD) from various angles. The measurement
signal is then modulated, amplified, and transmitted to analog drive board for further processing. The
scatter measured by the photodiode can be divided into three angles: low angle scatter, medium angle
scatter and high angle scatter. The type and size of the cell can then be determined by the distribution
of scatter intensity on these angles.

8-28
Hardware System

Figure 8-14 Electrical connection diagram of the optical system

Figure 8-14 shows the electrical connection diagram of the optical system. The analog
drive board controls the laser. When the PDs detect the light signal, they will transmit current
signals through the signal wire to the preamplifier. After I/V conversion, modulation and
amplification, the signals will be transmitted through the signal wire to the analog drive board for
further processing. A protective microswitch is connected between the analog drive board and
the laser analog board. When the optical system housing is opened, the microswitch will be
turned off, and the laser will stop working. Therefore, in order to test the laser, press the
microswitch manually to connect the electrical circuit (make sure the wire is connected to the C
pin and ON pin of the microswitch).

8.5.2 Functions of Optical Boards


SF PD board and FS/SS PD board
The SF PD board and FS/SS PD board are mainly used for photoelectric conversion, which
converts the light irradiated on the PD to electrical current and transmits the current signal to the
preamplifier for further processing.

Preamplifier
Overview

The preamplifier is mainly used for photoelectric conversion and amplification of the three
scatters from the flow cell (forward scatter (FS, also called low angle signal), side medium angle
scatter (SS, also called medium angle signal) and side high angle scatter (SF, also called large angle
signal)). The FS/SS preamplifier and the SF preamplifier share a common PCB, and achieve the
required amplification through welded resistors.

Note: LAS and FS refer to low angle signal, MAS and SS refer to medium angle signal, and WAS
and SF refer to large angle signal, unless otherwise noted. Corresponding abbreviations refer to the
same objects.

8-29
Hardware System

Function
The function of the preamplifier includes power modulation, I/V conversion and signal
modulation.
Power modulation: filters the -12V power supplied by the analog board. The filtered ripples
will be less than 50 mV.
Signal modulation: converts the current signals to voltage signals by I/V. The signals are then
sent to the amplification unit and processed to meet the input requirements of the analog drive
board.

Figure 8-15 Functional block diagram of the preamplifier

Laser Control Board


Overview
The laser control board controls the laser to provide stable and moderate-intensity laser.

Function
The functions of the laser control board include power modulation, laser driving current
monitoring and laser power control.
Power modulation: filters the 12V power supplied by the analog board. The filtered ripples
will be less than 100mV.
Laser driving current monitoring: measures the electrical current of the laser and send the
results to the analog board for monitoring.

Laser power control: the laser is controlled by a constant power control method. The laser
output power is monitored in real time by a photodetector inside the laser. The result forms a
loop system by negative feedback to achieve constant output power. The power is controlled
within the range of 3 mW~5 mW by adjusting the potentiometer VR1 on the board.

8-30
Hardware System

Figure 8-16 Functional block diagram of the laser control board

Adjusting laser driving current: when a laser overcurrent problem is reported, it is usually
necessary to adjust the adjustable potentiometer VR1 on the laser analog board to adjust the laser
current. when adjusting, observe the laser current changes in the Voltage and Current screen in the
Analyzer Status screen. If the current does not change and is not zero when VR1 is being adjusted, then
either the laser analog board or the laser fails. Otherwise the problem may be caused by wire failure,
and it is required to check the wires before adjustment. It is also necessary to adjust the current after
replacing the laser analog board to keep the current within the acceptance. The adjusting method is the
same with above.

8.5.3 Troubleshooting
Table 8-14 Troubleshooting and solutions of optical problems

No. Symptom Possible Cause Solution


1 Laser does Power/microswitch 1. Check if the connecting wire between the
not work /laser abnormality optical system and analog drive board is loose or
broken. If loose, reconnect the wire. If broken,
replace the wire. Otherwise, proceed to the next
step.
2. Check if the microswitch is working
properly. Press the microswitch with power off.
Measure the continuity between ON and C pin
with a multimeter. If the measured resistance is
greater than 10 ohms, replace the microswitch.

8-31
Hardware System

Otherwise, proceed to the next step.


3. Check the power supply of the optical
system by measuring the analog drive board at J9.1
(AVSS-12.6~-11.4V), J9.2 (AVCC
11.4~12.6V), J9.13 (AVSS-12.6~-11.4V), J9.17
(AVCC11.4~12.6V), J9.20 (AVDD
4.75~5.25V), J9.4~J9.16 (GND). If any
measurement is abnormal, check if there is
problem in the power board or the analog drive
board. Otherwise, proceed to the next step.
4. Check if the analog drive board J9.18 is
low. If not, check if the power board and the
analog drive board are working properly.
5. If 1 to 4 are all excluded, then the problem
is in the power board or the analog drive board.
Replace the laser analog board. If the problem
persists, replace the optical system.

2 No signal Power/control 1. Check if the connecting wire between the


output signal/other abnormality optical system and analog drive board is loose or
from FS, broken. If loose, reconnect the wire. If broken,
SS and SF replace the wire. Otherwise, proceed to the next
channel step.
2. Check the power supply of the optical
system by measuring the analog drive board at J9.1
(AVSS-12.6~-11.4V), J9.2 (AVCC
11.4~12.6V), J9.13 (AVSS-12.6~-11.4V), J9.17
(AVCC11.4~12.6V), J9.20 (AVDD
4.75~5.25V). If any measurement is abnormal,
check if there is problem in the power board or the
analog drive board. Otherwise, proceed to the next
step.
3. Check if the analog drive board J9.18 is
low. If not, check if the power board and the
analog drive board are working properly.
4. If all the above problems are excluded,

8-32
Hardware System

replace the preamplifier and check if the problem


is solved. If the problem persists, replace the
optical system.

8.6. Touchscreen Drive Board

8.6.1 Overview
The touchscreen drive board provides the interface between the touchscreen and the control board,
converting the user's touch operation to identifiable signals for the control board. The touchscreen drive
board needs to be adjusted before use.

8.6.2 Components
The core of this board is a 4-wire touchscreen control chip TSC2004IRTJR, which communicates
with the control board through the I2C interface to transmit the position information of the touch
points.

Figure 8-17 Touchscreen connection

8.6.3 Troubleshooting
Table 8-15 Troubleshooting and solutions of the touchscreen

No. Symptom Possible Cause Solution


1 The Touchscreen analog 1. Check if the control board is working
touchscreen board properly. If not, please solve the control board
will not failure/Touchscreen problem first.
respond to failure/Loose 2. Check if the connecting wire between the
touch connection touchscreen analog board and the control board is
loose or broken. If loose, reconnect the wire. If
broken, replace the wire.
3. If the problems in 1 and 2 are excluded,
then replace the touchscreen analog board and see
if the problem is solved.

8-33
Hardware System

4. After the replacement, if the problem


persists, replace the touchscreen.
2 The cursor Loose connection 1. Check if there is any crack on the
can only move between the touchscreen. If there is, it will be necessary to
horizontally or touchscreen analog replace the touchscreen.
vertically board and the 2. Reconnect the wire between the
when touching touchscreen/Broken touchscreen analog board and the touchscreen.
the screen touchscreen
3 The cursor Un-calibrated/Brok 1. Perform touchscreen calibration in
can not move en touchscreen accordance with the calibration procedure from the
to certain area screen (if the deviation is too much between the
touch point and the actual cursor position to enter
the touchscreen calibration screen, use a USB
mouse to enter the screen and start the calibration
procedure).
2. If the problem persists, check if the
touchscreen is broken. If so, please replace the
touchscreen.

8.7. Indicator Board

8.7.1 Overview
The indicator board provides the user with sound or light indications to inform the current
analyzer status.

Figure 8-14 Indicator Board

8.7.2 Components
The indicator board consists of red, yellow and green indicators, control circuits and buzzer drive
circuit. The indicator board does not need to be adjusted before use.

8-34
Hardware System

8.7.3 Troubleshooting
Table 8-16 Troubleshooting and solutions of the indicator board

No. Problem Description Troubleshooting and solutions


Type
1 Indicator Dim indicator There are 7 LEDs for each color. A dim color
indicated some LED(s) of this color is damaged.
Remove the indicator board and check if all the 7
LEDs are illuminated. If the number of illuminated
LEDs is less than 7, then the board is damaged and
needs to be replaced. IF not, please measure the
voltage between pin 6 and pin7 at J1 of the indicator
board. If the voltage is less than 4.5V, please check if
the 5V power supply of the control board is working
properly.
The indicator is 1. Check if the control board is working properly.
not illuminated. If not, please solve the control board problem first;
2. Check if the connecting wire of the indicator
board is loose or broken. If loose, reconnect the wire.
If broken, it is necessary to replace all the front panel
signal wires together.
3. If there is no problem in the wires, please
replace the indicator board.

8.8. Motor, Photocoupler and Microswitch

8.8.1 Overview
The motor is used for driving assemblies such like sampling assembly and syringe assembly. The
photocoupler is used for detecting the motor position. The microswitch is used for starting the blood
test process. The schematic diagram of the motor and the photocoupler is shown below.

8-35
Hardware System

8.8.2 Troubleshooting
Table 8-17 Troubleshooting and solutions of motor, photocoupler and microswitch

No. Problem Description Troubleshooting and solutions


Type
1 Motor The motor does 1 Check the analog drive board. If there is problem
not turn with the analog drive board, solve it first.
2 Check there is any loose or broken wire between the
motor and the analog drive board. If there is, replace
the corresponding wire.
3 If there is no problem in the wires, replace the
motor.
The motor 1 Check the analog drive board. If there is problem
won't stop at with the analog drive board, solve it first.

the specified 2 Check there is any loose or broken wire between the
photocoupler and the analog drive board. If there is,
position
replace the corresponding wire.
2 Photocouple The motor can
3. If there is no problem in the wires, replace the
r not move to
photocoupler.
certain position

8-36
Hardware System

8.9. Liquid sensor board

8.9.1 Overview
The liquid sensor board is used to detect whether there is liquid in the tube, judge whether
there is liquid or not by the refractive ratio for the refractive ratio is not the same when there is
liquid or not.

Figure 8-15 Liquid sensor board

8.9.2 Composition
The main part of the board is photocoupler, there is a LED indicator at the back of the board,
if there is not barrier between the photocoupler transmitting tube and receiving tube, the LED
light is off; if the photocoupler is blocked, the LED light is on. When testing the board function,
use the card to perform photocoupler barrier testing.

8.9.3 Troubleshooting
When testing the reagent, if there is reagent, the board TP4 output is low electrical level
(lower than 0.8V) and the LED light is off, if there is no reagent, output the high electrical level
(higher than 2V) and the LED light is on, when the error occurs, troubleshoot by the LED light
status and whether there is reagent at the testing point.

8-37
9 Heating System

9.1. Heating System Overview


The heating system consists of diluent heating system and optical heating system. The
diluent heating system provides the best temperature range for the RBC bath and the WBC bath.
The optical heating system provides the temperature range for normal operation of the optical
instruments.
The diluent heating system consists of temperature detection assembly and preheat
assembly. A diluent sensor and a preheat sensor work together to detect and control the
heating temperature of the preheat assembly.
The optical heating system consists of power assembly and fan assembly. The optical
system is heated by the heat generated by the power board. When the optical system
temperature exceeds a specified limit, a temperature sensor inside the Optical System will start
the fan assembly, which draws heat from the bottom of the power assembly and controls the
temperature of the Optical System.
The temperature ranges of each detection point are listed below:
Name Temperature Range C
Temperature of the diluent 10~40
detection assembly
Temperature of preheat Variable. A range can be calculated by
assembly the diluent temperature
Internal temperature of optical 15~40
system

9.2. Heating System

9.2.1 Diluent Heating System

Structure diagram
1. Temperature detection assembly
Symbol:

9-1
Heating System

Appearance:

Function: a. Determine if the diluent temperature is within [10C, 40C]. If not, the analyzer
will stop working and beep; b. provides the diluent temperature to calculate the bath temperature.

2. Reagent preheating unit:


Symbol:

Appearance:

Function: perform and control heating of diluent, and detect the bath temperature after heating.

9-2
Heating System

9.2.2 Optical heating system


Optical System
Temperature Testing
Unit

J10
J17
Analog Drive J18 Fan System

Board J19

Function:
Power assembly: continuously heats the optical system;
Optical System: detects the temperature and determine whether to work;
Fan assembly: cools the optical system. Starts when the optical system temperature is
higher than 30, and stops when the optical system temperature is lower than 25.

9-3
10 Mechanical System
10.1. Mechanical System Overview
This section lists the locations of major analyzer components for the service personnel to
remove and replace the components. The diagrams in this manual is based on BC-5150.
BC-5000 and BC-5150 are basically the same.

Front view

Figure 10-1 Front view of the main unit

1 ---- Display 2 ---- Power/Status indicator


3 ---- Key supporting pad (wipe baffle) 4 ---- Sample probe
5 ---- [Aspirate] key

10-1
Mechanical System

Figure 10-2 Front view of the main unit (cover open)

1 --- Fluidic valve 2 --- Syringe


3 --- Metering pump 4 --- [Aspirate] key
5 --- Sampling assembly 6 --- Pressure & Temperature testing unit

Back view

10-2
Mechanical System

Figure 10-3 Back view of the main unit

1 --- Diluent Inlet 2 --- Waste Outlet


3 --- Waste sensor 4 --- Power input socket
5 --- Power switch 6 --- Power backplane
7 --- Back panel

10-3
Mechanical System

Figure 10-4 Back view of the main unit (internal structure)

1 --- Analog drive board 2 --- Pinaster board


3 --- Fan assembly

Left view

Figure 10-5 Left view of the main unit

10-4
Mechanical System

1 --- Access door 2 --- Panel Module


3 --- Left door assembly 4 --- Network interface and USB port

Figure 10-6 Left of the analyzer (left door open)

1 --- Left door 2 --- DIFF reagent bottle


3 --- LH reagent bottle 4 --- Front Panel Module
5 --- Network port, USB port

10-5
Mechanical System

Left view

Figure 10-7 Right view of the main unit (right door open)

1 --- Fluid port 2 --- Waste water pump subassembly


3 --- Vacuum chamber assembly 4 --- valve
5 --- Temperature sensor 6 --- Sampling assembly
7 --- Panel Module 8 --- RBC unit
9 --- WBC unit

10.2. Components

10.2.1 Introduction
This section provides exploded view of the analyzer and material IDs for the service
personnel to understand the relationship between the components when removing and
replacing the components.

Note

All the material IDs listed in the Material ID column of all the parts lists are only used for the
service personnel to search the corresponding spare part ID. Please provide the spare part ID when
purchasing a spare part.

10-6
Mechanical System

10.2.2 Overall
Exploded view

Parts list
No. Material ID Material Description Spare Part ID Comments

1 Panel Module (BC-5150) / /


115-014325-00
3 Top Cover (BC-5150) / /
1 Panel Module(BC-5000) / /
115-014323-00
3 Top Cover (BC-5000) / /
4 / The M3X8 Combination Bolt / /

2 Right Door / /

5 115-015670-00 Assembly, Left door / /

7 Main Unit / /

6 / Screw, Flat Head Phillips M3X6 / /

8 115-016034-00 DIFF Lyse Cap Assembly 115-016034-00 /

9 115-016035-00 LH Lyse Cap Assembly 115-016035-00 /

M-68D CAP Component for


10 115-007635-00 801-3201-00053-00 /
Diluent
11 115-013091-00 Waste Cap assm 115-013091-00 /

12 3102-20-69094 Diluent Assembly holder 801-3110-00167-00 /

10-7
Mechanical System

10.2.3 Main unit


Exploded view

10-8
Mechanical System

10-9
Mechanical System

Parts list
No. Material ID Material Description Spare Part ID Comments

1 / Mainframe / /
See Section
2 / syringe assembly /
11.2.6
3 115-014601-00 1ml Pump(eject) 115-014601-00 /

4 115-015678-00 Switch assembly 115-015678-00 /

5 / 3-way Valve (Mindray) 801-3201-00003-00 /

6 0030-30-07587 2-way Valve (Mindray) 0030-30-07587 /


mirco solenoid valve of
7 115-007667-00 two-way(higher operating 115-007667-00 /
pressure)
8 / exoteric sampling keystroke 801-3100-00194-00 /
8 043-002703-00 exoteric sampling keystroke 043-002703-00 /

9 / key support plate / /

10 115-014598-00 200ul pump 115-014598-00 /

11 115-017730-00 liquid detect assembly 115-017730-00 /

12 115-015673-00 Vacuum/Pressure Chamber 115-015673-00 /

13 / RBC isolated washer / /


RBC shielding bottom plate
14 / / /
welding piece
15 115-015672-00 Reagent preheating unit 115-015672-00 /
See Section
16 / RBC count pool module /
11.2.8
17 043-000711-00 filter 043-000711-00 /

18 / Isolation chamber clamp 801-3003-00045-00 /

19 042-007065-00 RBC shielding box 042-007065-00 /

20 / WBC heat preservation box / /

21 115-015676-00 Sample Probe Assembly 115-015676-00 /

22 / right door / /

23 115-014726-00 TAKASAGO value assem 115-014726-00 /


See Section
24 / GHB count pool module /
11.2.8
25 115-015675-00 LVMvalve assembly 115-015675-00 /

waste water pump See Section


26 / /
subassembly 11.2.10
Temperature detection
27 115-015677-00 115-015677-00 /
assembly
See Section
28 115-015680-00 Power Assembly 15-015680-00
11.2.13
29 / reagent bottle plate cosmetic / /

10-10
Mechanical System

No. Material ID Material Description Spare Part ID Comments

piece
30 115-016034-00 DIFF Lyse Cap Assembly 115-016034-00 /

31 / Reagent bottle,500ml / /

32 / Reagent bottle,220ml / /

33 115-015681-00 Assembly, Left door / /

34 115-016035-00 LH Lyse Cap Assembly 115-016035-00 /

35 / Plate, fixing liquid detect board / /

36 051-001621-00 Liquid detect board PCBA 051-001621-00 /

37 043-000829-00 Reagent detection tube 043-000829-00 /


Male Lure Lock Ring,For MTLP
38 / / /
or LC23,Green
39 / Plate,fixing liquid fetect board / /

40 051-001621-00 Liquid detect board PCBA 051-001621-00 /

41 043-000829-00 Reagent detection tube 043-000829-00 /

42 / Optical System 115-018419-00 /

43 115-017923-00 assembly, fan 115-017923-00 /


See Section
44 / Assembly, power back plate /
11.2.11
45 / back plate / /

/ USB grounging sheet / /


46
/ Conductive Fabric Gasket / /
Pinaster board(5
47 051-001159-00 051-001159-00 /
ALL_AM1808)
48 / Analog Drive Board PCBA 115-018411-00

49 115-017477-00 3107 wire assembly / /

50 115-017475-00 tube package / /


Secure Digital Memory Card
51 023-000549-00 023-000549-00 /
8GB
Lithium battery 3V 35mAh
52 / 801-3100-00226-00 /
D12.5*2.0

10-11
Mechanical System

10.2.4 Panel Module (BC-5150)


Exploded view

Parts list
No. Material ID Material Description Spare Part ID Comments

1 043-002706-00 BC-5150 front cover 043-002706-00 /

2 045-000016-01 Touch Panel Defend cushion / /

3 / Touch Panel 801-3110-00184-00 /

4 / screen module(BC-5150) /

5 / The M3X8 combination Bolt /

6 / touch panel shield box /


115-018418-00
7 051-000881-00 6301 touch control board PCBA /
M4 Strap Natural Nylon Color
11 / /
94ULV-2
8 051-000675-00 Indication board PCBA 051-000675-00 /

9 / shield(5150) / /

Cross pinhead screw with washer


10 / / /
M3X10

10-12
Mechanical System

10.2.5 Panel Module (BC-5000)


Exploded view

Parts list
No. Material ID Material Description Spare Part ID Comments

1 043-002705-00 BC-5000 front cover 043-002705-00 /


2 045-000016-01 Touch Panel Defend cushion / /

3 021-000005-00 Touch Panel 801-3110-00184-00 /

4 / screen module(BC-5000) /
5 / The M3X8 combination Bolt /

6 / Touch panel shield box /


115-018417-00
7 051-000881-00 6301 touch control board PCBA /
M4 Strap Natural Nylon Color
11 / /
94ULV-2
8 051-001062-00 Indicator board PCBA 051-001062-00 /

9 / Shield(5000) / /
Cross pinhead screw with washer
10 / / /
M3X10

10-13
Mechanical System

10.2.6 Syringe Assembly


Exploded view

Parts list
No. Material ID Material Description Spare Part ID Comments

1 024-000366-00 Stepping motorSST42D2120 024-000366-00 /


2 / coupling / /
3 / Rubber Ring + Positioning Sleeve / /
4 041-005167-00 special bolt 3 041-005167-00 /

5 115-011902-00 10ml syringe(3107) 115-011902-00 /


6 / The M3X8 combination Bolt / /
7 / the fixed plate of two injecter / /
8 115-012708-00 250ul syringe(with nozzle) 115-012708-00 /

Syringe Motor Position Sensor


9 / 801-3003-00015-00 /
Assembly
Screw, Pan Head W/Washer
10 / / /
Phillips M3X6
11 / M3X12 Hexagon socket screws / /

10-14
Mechanical System

10.2.7 Sample Probe Assembly


Exploded view

Parts list
No. Material ID Material Description Spare Part ID Comments

1 / Elevator Motor(2S42Q-05640A) 801-1805-00013-00 /


2 / Upper Positioning Sleeve / /
3 / M3X5 Hexagon Socket Set Screw / /
4 / Transducer discreteness 801-3001-00055-00 /
5 / SCREW M3X4 / /
Syringe Motor Position Sensor
6 / 801-3003-00015-00 /
Assembly
7 / Lower Positioning Sleeve / /
8 / probe wipe 801-3102-00057-00 /
Cross pinhead screw with washer
9 / / /
M3X10
10 / probe wipe clamp 801-3900-00096-00 /
11 / Sample Probe 801-3101-00002-00 /
12 / The M3X8 combination Bolt / /
13 / probe press plate / /

10-15
Mechanical System

10.2.8 RBC count pool module


Exploded view

Parts list
No. Material ID Material Description Spare Part ID Comments

1 / Add liquid connector /


115-018414-00
2 / pool cover /
M3X6 Stainless Steel Cross Recess
3 / / /
Pinhead Screw
4 / Count pool support plate / /
5 / Count pool adjusting plate / /
6 / RBC count pool /
7 / Front bath washer 115-018412-00 /
9 / RBC back pool /
8 045-000809-00 Ruby Red Cell Counter(D50um) 045-000809-00 /

10-16
Mechanical System

10.2.9 HGB count pool module


Exploded view

Parts list
No. Material ID Material Description Spare Part ID Comments

1 / add liquid connector /


115-018413-00
2 / pool cover /
M3X6 Stainless Steel Cross Recess
3 / / /
Pinheads Screw
4 / Count pool support plate / /
5 / Count pool adjusting plate / /
6 043-002923-00 HGB count pool 043-002923-00 /
7 / HGB amp module 115-018415-00 /

10-17
Mechanical System

10.2.10 Pump Assembly


Exploded view

Parts list
No. Material ID Material Description Spare Part ID Comments

1 / pump mounting plate / /


2 / The M3X8 combination Bolt / /
3 / Rubber Ring Positioning Sleeve / /
4 / Rubber Ring / /
5 / Rotation (Waste) pump /
115-018416-00
6 / cable of waste pump /

10.2.11 Power Backplane Assembly


Exploded view

10-18
Mechanical System

Parts list
No. Material ID Material Description Spare Part ID Comments

1 / power back plate / /


2 / LockNut,1/4-28UNF,Red Nylon / /
Lock Nut, Panel
3 / / /
Mount,1/4-28UNF,White Nylon
4 / Coded Lock Ring, Red / /
Coded Lock Ring, For FTLLB or
5 / / /
FTLB,White
6 / Female Luer,1/4-28UNF,1/8ID / /
7 / BNC socket / /
8 / Screw, Flat Head Phillips M3X6 / /
Filter power 115/250VAC6A panel
9 0030-10-13055
mount
10 M07-00131F--- FUSE Time-lag 250V 3.15AD5X20

10-19
Mechanical System

10.2.12 Optical System


Exploded view

Parts list
No. Material ID Material Description Spare Part ID Comments

1 051-001142-00 3107 Preamplifier 051-001142-00 /


2 / Shield of Preamp PCBA / /
3 M07-00143S--- SWITCH / /
4 / SUS316 Flat Washer - A Class 4 / /
5 / Laser control board / /
6 / Coverplate of Optics Shield / /

10-20
Mechanical System

10.2.13 Power Assembly


Exploded view

Parts list
No. Material ID Material Description Spare Part ID Comments

1 / power cover plate / /


2 051-001319-00 3107 Power Source PCBA 051-001319-00 /
3 / Power bottom plate / /
4 / The M3X8 combination Bolt / /

10.3. Removal and Installation

10.3.1 Tools
The following tools may be needed during removal and replacement of components:
Crosshead screwdriver (107)
Flathead screwdriver
Tweezers
Pliers
Cutting pliers
Hex wrench set

10.3.2 Preparation for Disassembly


Before disassembling the analyzer, please make the following preparations:

10-21
Mechanical System

Stop the blood tests. Adjust the sample probe to the horizontal sampling position. Shut
down the analyzer and disconnect all the connections with accessories and peripherals.
Disconnect the external power supply.

All the analyzer components and surfaces are potentially infectious. Take proper
protective measures for operation and maintenance.

The reagents are irritating to eyes, skin and diaphragm. Wear proper personal protective equipment (e.g.
gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory.
If the reagents accidentally spill on your skin, wash them off with plenty of water and if necessary, go see a
doctor; if the reagents accidentally spill into your eyes, wash them off with plenty of water and
immediately go see a doctor.
Please eliminate static electricity before disassembly. While removing the components with electrostatic
sensitive mark, please wear protective equipment such like an antistatic wrist strap or antistatic gloves
to avoid ESD damage to the components.
During reassembly, please connect the wires correctly and keep them in proper positions to avoid short
circuit caused by damaged wires.
Use screws of suitable models during reinstallation. Using wrong screws may result in equipment damage.
Furthermore, during usage after reinstallation, a wrong screw may become loose and fall off, resulting
in unexpected product damage or personal injury.
Please disassemble the equipment in the correct order. Failure to do so may result in irreversible damage
to the equipment.
Please make sure all connections have been disconnected before disassembling the components. Be careful
not to break the wires or the connectors during disassembly.
Please store the removed screws and other parts in separate places for reinstallation purpose. Be careful
not to drop, contaminate or lose these parts.
During disassembly, separate the materials by module to avoid misusing or missing materials during
reassembly.
During reassembly, please assemble first the components then the main unit. Be careful with the wire
connections. Place the wires in proper position.

10.4. Disassembling the Main Unit

During the disassembly, make sure the site is smooth without foreign materials to avoid screen
scratches.

10-22
Mechanical System

All operations must be done by professionals. Insulating gloves must be worn when servicing.
After assembly, check all the fluidic tubes. Folding is strictly prohibited.

10.4.1 Removing the Back plate


Place the analyzer flat on the table as shown below. Unscrew the 4 M38 combination
screws and remove the back plate.

10.4.2 Removing the Analog drive board PCBA


1. Remove the back plate in accordance with Section 10.4.1.
2. Remove the 3 M38 combination screws as shown below. Remove the top cover of the
shield box and disconnect the RBC_PLT signal wire connector.

3. Remove all the cables from the analog drive board as shown below. Unscrew the 8
M38 combination screws and remove the analog drive board.

10-23
Mechanical System

Installation:
Reverse the removal procedure.

Verification: 1. Check all the connections on the analog drive board and make sure there is
no mistakes.
2. Start the analyzer and power on the analog drive board. Check if the analog drive
board power and indicators are working properly.
3. Perform blank background count. If no alarm occurs, then the analog drive board is
successfully replaced. Otherwise troubleshooting is needed.

10.4.3 Removing the Pinaster board or the SD card


1. Remove the back plate in accordance with Section 10.4.1.
2. Remove all the cables from the Pinaster board as shown below. Unscrew the 6 M38
combination screws and remove the analog drive board.

3. As shown below, unscrew the 2 M38 combination screws and remove the USB ground
plate and conductive foam as a unit.

10-24
Mechanical System

4. Remove the SD card from the board.


Installation:
Reverse the removal procedure.
Verification: 1. Check all the connections on the Pinaster board and make sure there is no
mistakes.
2. Start the analyzer and power on the Pinaster board. Check if the Pinaster board
power and indicators are working properly.
3. Perform blank background count. If no alarm occurs, then the Pinaster board is
successfully replaced. Otherwise troubleshooting is needed.

10.4.4 Removing the power backplate Assembly


1. Remove the power backplate in accordance with Section 10.4.1.
2. Place the analyzer flat on the table as shown below. Unscrew the 4 M3x8
combination screws. Remove the diluent and waste tubes, and cables and ground wires
connected with the power board.

10-25
Mechanical System

During installation, verify that the ground wire is connected to the correct ground pin properly.

10.4.5 Removing the Fan Assembly


1. Remove the power backplate assembly in accordance with Section 10.4.4.
2. Remove all the cables from the fan as shown below. Unscrew the 2 M38
combination screws and remove the fan assembly.

Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the electrical connections are correct.
3. After startup, press the button from the system menu, select
MaintenanceSelf-testFan Self-testclick Spin. When the Spin column
displays spin, check if the fans on the both sides of the back plate power switch can spin
properly. If so, click the Stop button and check if the fan is stopped when the Stop
column displays stop.

10.4.6 Removing the Right Panel


Place the analyzer flat on the table as shown below. Unscrew the 2 M3x8 combination
screws and remove the back plate.

10-26
Mechanical System

10.4.7 Removing the RBC count pool module(FRU)


1. Follow step 1 and 2 in Section 10.4.2 to remove the top cover from the analog board
shield box and disconnect the RBC_PLT signal wire connector.
2. Remove the right door in accordance with Section 10.4.6.
3. As shown below, unscrew the 2 M38 combination screws and remove the RBC shield
box.

4. As shown below, rotate the RBC back pool 90 counterclockwise and remove the RBC
back pool. Use tweezers to remove the front bath washer and the Ruby Red Cell Counter(D50um).

10-27
Mechanical System

5. As shown below, rotate the secondary RBC bath 90 counterclockwise and remove the
secondary RBC bath. Use tweezers to remove the primary bath washer and the microsensor.

Installation:
Reverse the removal procedure.

Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. Verify normal operations by starting the analyzer.

Before removing the reaction bath shield box, please adjust the sample probe position so that the
sample probe leaves the shield box. Otherwise the sample probe may bend or hurt the operator.
Install the Ruby Red Cell Counter(D50um) so that the concave of the sensor is facing the center of
the RBC count pool.
pool

10.4.8 Removing the Ruby Red Cell Counter(D50um)


1. Follow step 2 and 3 in Section 10.4.7 to remove the RBC shield box.

10-28
Mechanical System

2. As shown below, cut off the straps which fasten the tube between the reaction bath to the
isolation chamber filter. Rotate the secondary RBC bath 90 counterclockwise and remove the
secondary RBC bath.

3. As shown below, unscrew the 2 M3 screws which fasten the RBC count pool module.
Remove the RBC count pool module. Use tweezers to remove the ront bath washer and the Ruby
Red Cell Counter(D50um).

Note: Use angled tweezers to push the Ruby Red Cell Counter(D50um) out from the RBC
count pool center

Before removing the reaction bath shield box, please adjust the sample probe position so that the
sample probe leaves the shield box. Otherwise the sample probe may bend or hurt the operator.
Install the Ruby Red Cell Counter(D50um) so that the concave of the sensor is facing the center of
the RBC count pool.
pool

10.4.9 Removing the HGB amp module


1. Remove the right door in accordance with Section 10.4.6.
2. As shown below, unscrew the 2 M38 combination screws and remove the WBC heat
preservation box.

10-29
Mechanical System

3. Follow the steps in Section 10.4.1 to remove the back plate, and disconnect the HGB unit
connector.
4. As shown below, unscrew the 2 M3 screws which fasten the pool cover. Remove the lower
part of the HGB count pool module. Rotate the bath counterclockwise 90 and remove the HGB
amp module.

Installation:
Reverse the removal procedure.

Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. Verify normal operations by starting the analyzer.

Before removing the WBC heat preservation box,, please adjust the sample probe position so that
the sample probe leaves the shield box. Otherwise the sample probe may bend or hurt the
operator.

10-30
Mechanical System

10.4.10 Removing the Liquid detect board PCBA


1. Place the analyzer flat on the table as shown below. Unlock and open the left access door.
Loosen the strap which fastens the reagent bottle tubes and move the tubes towards the top end of
the reagent bottle plate cosmetic piece. Remove the reagent bottle (with the tubes) and put it away.

2. Unscrew the M38 compound screw, remove the liquid sensor board installation kit and
PCBA assembly of the liquid sensor board.

3. Remove the pipe and wires of the liquid sensor board and cancel the liquid sensor board
PCBA.
Note: During the installation, connect the PCBA connector at the square hole to the DIFF
reagent pipe, connect the PCBA connector at the round aperture to the LH reagent pipe.

10.4.11 Removing the Reagent Bottle plate cosmetic piece

10-31
Mechanical System

1.Perform step1and step 2 of section 10.4.10, take off the liquid sensor board installation kit
and PCBA assembly of the liquid sensor board.
2.Unscrew the 3 M38 combination screws and remove the reagent bottle plate cosmetic
piece.

10.4.12 Removing the preheat Assembly


1. Follow step 1 and 2 in Section 10.4.9 to remove WBC heat preservation box.
2. As shown below, remove the tubes from the Reagent preheating unit . Unscrew the 2 M3x8
combination screws and remove the Reagent preheating unit . Pull the connector out from the
hole. Disconnect the cable connector of the Reagent preheating unit (leave the connector at the
left side for installation purpose).

10-32
Mechanical System

Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the electrical connections are correct;
3. After startup, press the button from the system menu, select
MaintenanceTemp.& Press. Calibration and check if the preheat bath temperature
column is displayed in red.
Note: If the wire head retracts back from the hole, please follow the steps in Section 11.4.10
to remove the reagent bottle baffle.

Before removing the WBC shield box, please adjust the sample probe position so that the sample
probe leaves the shield box. Otherwise the sample probe may bend or hurt the operator.

10.4.13 Removing the Electromagnet Pinch Valve Assembly


1. Follow step 1 and 2 in Section 10.4.9 to remove the WBC shield box.
2. As shown below, remove the tubes from the pinch valve. Unscrew the 2 M3x12 screws and
remove the valve assembly. Pull the connector out from the hole. Disconnect the cable connector
of the Reagent preheating unit (leave the connector at the left side for installation purpose).

Installation:
Reverse the removal procedure.

Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the electrical connections are correct;
3. After startup, press the button from the system menu, select

10-33
Mechanical System

maintenanceSelf-testValve Self-test and click on valve 18 to see if it is working


correctly.
Note: If the wire head retracts back from the hole, please follow the steps in Section 11.4.10
to remove the reagent bottle plate cosmetic.

Before removing the WBC shield box, please adjust the sample probe position so that the sample
probe leaves the shield box. Otherwise the sample probe may bend or hurt the operator.

10.4.14 Removing the Right Valve Assembly


1. Remove the right door in accordance with Section 10.4.6.
2. As shown below, remove the tubes from the valve which needs service. Unscrew the 2
M3x12 screws and remove the valve assembly. Pull the connector out from the hole. Disconnect
the cable connector of the Reagent preheating unit (leave the connector at the left side for
installation purpose).

Installation:
Reverse the removal procedure.

Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. After startup, press the button from the system menu, select
maintenanceSelf-testValve Self-test and click on the number (which is printed on the
fluidics separator) to see if the corresponding valve is working correctly.
Note: If the wire head retracts back from the hole, please follow the steps in Section 11.4.10

10-34
Mechanical System

to remove the reagent bottle plate cosmetic.

10.4.15 Removing the Pump


1. Remove the right door in accordance with Section 10.4.6.
2. As shown below, remove the tubes from the pump. Unscrew the 4 M3x12 screws and
washers and remove the pump assembly. Pull the connector out from the hole. Disconnect the
cable connector of the Reagent preheating unit (leave the connector at the left side for installation
purpose).

4. As shown below, unscrew the 4 M38 combination screws and remove the pump.

Installation:
Reverse the removal procedure.

Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.

10-35
Mechanical System

3. Verify normal operations by starting the analyzer.


Note: If the wire head retracts back from the hole, please follow the steps in Section 11.4.10
to remove the reagent bottle baffle.

10.4.16 Removing the Vacuum Chamber Assembly


1. Remove the right door in accordance with Section 10.4.6.
2. Remove the tubes from the vacuum chamber. Unscrew the 2 M38 screws and remove the
vacuum chamber assembly.

Installation:
Reverse the removal procedure.

Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.

3. After startup, press the button from the system menu, select

StatusTemp.& Pressure and check if the Vacuum column is displayed in red.


4. Verify normal operations by starting the analyzer.

10.4.17 Removing the Top Cover


As shown below, unscrew the 2 M38 screws. Lift and remove the top cover from the rear
edge.

10-36
Mechanical System

10.4.18Removing the Temperature detection assembly


1. Remove the top cover in accordance with Section 10.4. 17.
2. Remove the cable connectors from the temperature detection assembly.

Installation:
Reverse the removal procedure.
Verification: 1. Verify the tube connections and the electrical connections are correct.
2. After startup, press the button from the system menu, select
StatusTemp.& Pressure and check if the Diluent temperature column is displayed
in red.

10.4.19 Removing the Sampling Probe Assembly

10-37
Mechanical System

1. Remove the right door in accordance with Section 10.4.6.


2. Follow the steps in Section 11.4.17 to remove the top cover, and disconnect the cable
connectors of the motor and the upper/lower sensors from the sample probe assembly.
3. As shown below, remove the tubes from the sample probe. Unscrew the 4 M312 screws
and washers, and remove the sample probe assembly.

Installation:
Reverse the removal procedure.

Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. After startup, press the button from the system menu, select
MaintenanceSample Probe Debug to verify the three posiaons of the sample probe
(Initial position, Up"," Middle position and Down position of RBC bath and WBC
bath) and ensure the reliable operation of the sample probe.
4. Verify normal operations by starting the analyzer.

10.2.20 Replace the Horizontal Motor of the Sampling Probe

Assembly
1. Remove the sample probe assembly in accordance with Section 10.4.19.
2. As shown below, first remove 2 set screws, then remove the 4 M310 screws, the Lower
positioning sleeve and the motor.

10-38
Mechanical System

10.4.21 Replace the Vertical Motor of the Sampling Probe

Assembly
1. Remove the sample probe assembly in accordance with Section 10.4.18.
2. As shown below, unscrew the 1 M34 screw and remove the Transducer discreteness.

3. As shown below, first remove 2 set screws from the fixing hole, then remove the 4 M310
screws, the Upper positioning sleeve and the motor.

10.4.22 Removing the Probe wipe


1. After startup, press the button from the system menu, select
MaintenanceSample Probe Debug. Click on the Initial position, the RBC bath then
click on the Up to adjust the sample probe to above the RBC bath.

10-39
Mechanical System

2. Follow step 1 and 2 in Section 10.4.7 to remove the right panel and the RBC shield box.
3. As shown below, remove the probe wipe clamp and the Probe wipe. Disconnect the tube from
the Probe wipe.

Installation:
Reverse the removal procedure.

Verification: 1. Verify the tube connection is correct.


2. After startup, press the button from the system menu, select
MaintenanceSample Probe Debug to verify the three posiaons of the sample probe
(Initial position, Up", "Middle position and Down position of RBC bath and WBC
bath) and ensure the reliable operation of the sample probe.
3. Start the analyzer and perform the sample probe cleaning sequence. Check if any
fluid flows out from the bottom of the wipe.

10.4.23 Replacing the Sample Probe


1. After startup, press the button from the system menu, select
MaintenanceSample Probe Debug. Click on the Initial position, the RBC bath then
click on the Up to adjust the sample probe to above the RBC bath.
2. Remove the probe wipe in accordance with Section 10.4.22.
3. As shown below, remove the tubes from the sample probe. Remove the M3 screws
and the probe press plate, and remove the sample probe.

10-40
Mechanical System

Installation:
Reverse the removal procedure.
Verification: 1. Verify the tube connection is correct.
2. After startup, press the button from the system menu, select
MaintenanceSample Probe Debug to verify the three positions of the sample probe
(Initial position, Up"," Middle position and Down position of RBC bath and WBC
bath) and ensure the reliable operation of the sample probe.

10.4.24 Removing the Syringe Motor Position Sensor


1. Place the analyzer flat on the table. Follow the steps in Section 10.4.10 to remove the
reagent bottle cosmetic piece, and disconnect the cable connectors of the photocoupler assembly
of the sampling assembly motor.
2. As shown below, unscrew the 4 M34 screws and remove the Syringe Motor Position
Sensor Assembly.

10-41
Mechanical System

Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the electrical connections are correct;
3. After startup, press the button from the system menu, select
MaintenanceSample Probe DebugIniaal posiaon so that the sample probe goes
back into the initial position. Then click on RBC bath and WBC bath respectively to
verify normal operations of the sample probe.
4. Verify normal operations by starting the analyzer.

10.4.25 Removing the transducer discreteness


1. Follow the steps in Section 10.4.17 to remove the top cover, and disconnect the cable
connectors from the photocoupler assembly of the sample probe assembly motor.
2. Remove the right door in accordance with Section 10.4.6.
3. Move the sample probe by hand to above the WBC bath. Cut the strap fastening the sensor
assembly with diagonal pliers and remove the cable connectors.
4. Move the sample probe by hand to the front side. As shown below, unscrew the M34
screws, and remove the sensor assembly from the front side.

Installation:
Reverse the removal procedure.

Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the electrical connections are correct;

3. After startup, press the button from the system menu, select

MaintenanceSample Probe DebugIniaal posiaon so that the sample probe goes


back into the initial position. Then click on Up, Middle position and Down position

10-42
Mechanical System

respectively to verify normal operations of the sample probe.


4. Verify normal operations by starting the analyzer.

10.4.26 Removing the Panel Module


1. Place the analyzer flat on the table. Unlock and open the left access door.
2. Remove the right door in accordance with Section 10.4.6.
3. Remove the top cover in accordance with Section 10.4.17.
4. As shown below, unscrew the 6 M36 flathead screws. Remove the Panel Module and
place it flat on the table.

5. Follow the steps in Section 10.4.1 to remove the back plate, and disconnect the cable
connectors of the Panel Module from the Pinaster board.
6. Follow the steps in Section 10.4.10 to remove the reagent bottle baffle. Move the front
cover signal wire from the back to the front and remove it.

Note: During installation, the excess part of the signal wire shall be completely inserted into
the analyzer. Failure to do so will result in signal interference.

10.4.27 Removing the Indicator board PCBA


1. Follow step 1-4 in Section 10.4.26 to remove the top cover, and put it on the table. Remove
the cable connector from the indicator.
2. As shown below, unscrew the 2 M310 screws and remove the indicator board cover and
the indicator board PCBA.

10-43
Mechanical System

Installation:
Reverse the removal procedure.

Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections are correct;
3. Start the analyzer and verify the indicators can be illuminated.

10.4.28 Removing the 6301 touch control board PCBA


1. Follow step 1-4 in Section 10.4.26 to remove the top cover, and put it on the table. Remove
the cable connector from the touchscreen drive board.
2. As shown below, unscrew the 2 M38 combination screws and remove the Touch panel
shield box.

3. As shown below, remove the cables from the touchscreen. Unscrew the 2 M38
combination screws and remove 6301 touch control board PCBA.

10-44
Mechanical System

Installation:
Reverse the removal procedure.

Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections are correct;
3. Start the analyzer and verify normal operations of the touchscreen.

10.4.29 Removing the Touch Panel


1. Follow step 1 and 2 in Section 10.4.28 to remove the shield box and disconnect the cables.
2. As shown below, unscrew the 6 M38 combination screws which fasten the LCD Module,
and remove the Touch Panel (without disconnecting the cables).

3. Remove the touchscreen from the front cover.


Installation:
Reverse the removal procedure.

Verification: 1. Check if each connecting wire between the LCD Module and the Pinaster
board is locked.
2. Check if all the components are installed and fastened in position.
3. Calibrate the touchscreen.
4. Start the analyzer and verify normal operations of the touchscreen.

10-45
Mechanical System

10.4.30 Removing the LCD Module


1. Follow step 1-4 in Section 10.4.26 to remove the top cover, and put it on the table. Cut all
the straps fastening the screen assembly with diagonal pliers.
2. Follow step 1 and 2 in Section 10.4.28 to remove the Touch panel shield box and disconnect
the cables.
3. As shown below, unscrew the 6 M38 combination screws which fasten the LCD Module,
and the 2 screws which fasten the strap. Remove the LCD Module and disconnect all the cables
from the screen.

Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections are correct;
3. Start the analyzer and verify that the screen is working correctly.

10.4.31 Removing the Microswitch Assembly


1. Follow step 1-4 in Section 10.4.26 to remove the top cover, and put it on the table.
2. Remove the back plate in accordance with Section 10.4.1. Disconnect the connections
from the microswitch.
3. As shown below, unscrew the 2 M34 screws and remove the exoteric sampling keystroke
and the key support plate as a unit.

10-46
Mechanical System

4. As shown below, unscrew the 2 M38 combination screws and remove the microswitch
from the small hole in the front panel.

Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections are correct;
3. The Start key can be pressed down and released normally with an audible click.
4. Verify normal operations by starting the analyzer.

10.4.32 Removing the Syringe


1. Follow step 1-4 in Section 10.4.26 to remove the top cover, and put it on the table.
2. Remove the tubes from the syringe assembly.

10-47
Mechanical System

3. As shown below, unscrew the 4 M312 screws and flat washers. Remove the syringe
assembly and remove the cables from the syringe.

4. As shown below, unscrew the 3 M38 combination screws and special bolt 3, then remove
the syringe.

Installation:
Reverse the removal procedure.

Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections and the tube connections are correct;
3. Verify normal operations by starting the analyzer.

10.4.33 Removing the Syringe Motor


1. Follow step 1-3 in Section 10.4.32 to remove the syringe assembly.
2. As shown below, first remove the 2 M312 hex screws which lock the coupler, then
remove the 4 M312 hex screws which fasten the motor. Finally, remove the motor.

10-48
Mechanical System

Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections and the tube connections are correct;
3. Verify normal operations by starting the analyzer.

10.3.34 Removing the Syringe Motor Position Sensor Assembly


1. Follow step 1-3 in Section 10.4.30 to remove the syringe assembly.
2. As shown below, unscrew the 2 M36 screws which lock the coupler, and remove the
motor position sensor assembly.

Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections and the tube connections are correct;
3. Verify normal operations by starting the analyzer.

10.4.35 Removing the liquid detect assembly


1. Follow step 1-4 in Section 10.4.26 to remove the top cover, and put it on the table.

10-49
Mechanical System

2. Remove the tubes from the hydraulic detection assembly, and disconnect the cable
connections from the top.
3. As shown below, unscrew the 2 M38 combination screws and remove the liquid detect
assembly.

Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. Verify normal operations by starting the analyzer.

10.4.36 Removing the Electromagnetic Pump/Valve from the

Front Panel
1. Follow step 1-4 in Section 10.4.26 to remove the top cover, and put it on the table.
2. Remove the tubes from the assembly.
3. As shown below, unscrew the 2 M3x8 combination screws and remove the electromagnetic
pump/valve. Pull the connector out from the hole. Disconnect the cable connectors (leave the
connectors at the left side for installation purpose).

10-50
Mechanical System

Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. Verify normal operations by starting the analyzer.

10.4.37 Removing the Left Door Assembly


1. Place the analyzer flat on the table. Unlock and open the left access door.
2. As shown below, unscrew the 2 M38 combination screws and remove the left door
assembly.

Note: When installing the left door assembly, the hook at the bottom of the left door shall
engage the bottom plate so that the access door in the left door will not swing.

10-51
Mechanical System

10.4.38 Removing the Optical System


Please refer to Section 6.4.2 Replacement of the Optical System.

10.4.39 Removing the Optical System Cover


1. Remove the left door assembly in accordance with Section 10.4.37.
2. Remove the top cover in accordance with Section 10.4.17.
3. As shown below, unscrew the 4 M38 combination screws and remove the top cover of the
optical system shield.

4. Please refer to Section 6.4 Maintenance of the Optical System.

10.4.40 Replacing the RBC/WBC Isolation Chamber Filter


10-52
Mechanical System

1. Follow step 1 and 2 in Section 10.4.7 to remove the right panel and the RBC/WBC shield
box.
2. As shown below, unscrew the 1 M38 screw. Remove the clip on the chamber and
disconnect the tubes from the filter. Remove the isolation chamber filter.

Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections are correct;
3. Verify normal operations by starting the analyzer.

10.4.41 Replacing the Power Board PCBA


1. Remove the left door assembly in accordance with Section 10.4.37.
2. Remove the top cover in accordance with Section 10.4.17.
3. Remove all the cable connections from above the power box and place the DIFF reagent
bottle in a front position on the table.
4. As shown below, unscrew the 2 M38 combination screws Remove the power box
assembly and disconnect the connecting wires of the power socket from inside.

10-53
Mechanical System

5. As shown below, unscrew the 2 M38 combination screws on both sides and remove the
cover plate.

6. As shown below, unscrew the 4 M38 combination screws on both sides and remove the
power board PCBA.

Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections are correct;

10-54
Mechanical System

3. Verify normal operations by starting the analyzer.

10-55
11 Troubleshooting

Repair Guide
Error Trigger Related Potential
Error Related
Code Mechanism Factors Failure Troubleshooting Procedure
Screen

Comm 0x0100 1. Serial Analog drive Damaged / For occasional problems:


unicatio 0001 communicati board communicatio 1. Check the connection
n failure on protocol n module between the digital board J81
error. and the analog drive board J31.
Control board Damaged
2. No header, For regular problems:
communicatio
jumbo
n module 1. Check the connection
frames,
between the control board J81
insufficient Connection Loose wire
and the analog drive board J31.
frame length, between the
control board 2. Replace the related boards.
checksum
error, J81 and the

unrecognizabl analog board


e command J31

Control 0x0100 AD is not Control board / Voltage 1. Check if the parameters are
board 0002 within Analog drive & within normal ranges in the
failure [2.44-2.55] board Current Voltage & Current Status screen
Status 2. If so, replace the control
Power board
screen board
and connecting
wires 3. Otherwise, check if the power
board and connecting wires and
the connection between the
analog drive board J2 and
control board J86 are normal

4. If so, replace the analog drive


board

11-1
Troubleshooting

System 0x0100 Check the Button battery / / 1. Check if the button battery is
clock 0004 system clock installed on the digital board
failure is earlier than 2. If the battery is installed,
January 1, replace the battery and reset date
2000 Control board /
and time in the Setup screen.
Save and exit and reset the
analyzer

3. If the problem persists,


replace the control board

Diluent 0x0100 The diluent Reagent / Reagent 1. Verify there is no alarm in the
is used 0104 level is lower information Setup Reagent Setup screen
up than 4% setup screen 2. Verify there is sufficient
reagent in the diluent tank

LH lyse 0x0100 The LH lyse Reagent 1. Metering Reagent 1. Verify there is no alarm in the
is used 0105 level is lower information pump failure Setup Reagent Setup screen
up than 10% setup 2. Leaking or screen 2. Verify there is enough reagent
folding lyse in the reagent tank
related tubes
DIFF 0x0100 The DIFF
and valves
lyse is 0106 lyse level is
used up lower than
3%

Waste 0x0100 Float status Waste sensor Unable to Sensor 1. Check if the indicated
level is 0110 assembly change the Status information is as expected from
status

11-2
Troubleshooting

full correctly due screen the Sensor Status screen


to foreign 2. Check if the connecting wire
matter on the of the waste sensor is correct
float
3. Check if the float is able to
BNC change the status correctly
connector of
the waste
sensor is not
connected

Power 0x0100 56V power is Power board Damaged Voltage 1. Check if the Voltage &
failure 0201 not within power board & Current Current Status screen shows
[47, 60] Status expected information
screen 2. Check if the voltage at the
0x0100 12V power is Power cable Unreliable
test port of the related power
0202 not within power cable
board is as expected
[11.5, 12.5]
3. Check if the connection
0x0100 -12V power Analog drive Damaged
between the power board and
0203 is not within board board
the analog drive board is normal
[-12.5, -11.5]
4. Check if there is no short
0x0100 P12V power Peripherals and Short circuit circuit in analog peripherals,
0204 is not within connections connection power drive
[11.5, 12.5] peripherals and their respective
connections
0x0100 P24V power / /
0205 is not within 5. Replace the analog drive

[22.0, 29.0] board

preheat 0x0100 The preheat preheat bath Damaged Temp.& 1. Verify the corresponding
bath 0401 bath temperature Pressure temperature value from the
tempera temperature sensor Status Status screen
ture reading at the screen 2. First, check if the ambient
Temperature Loose wire
sensor analog drive temperature is overheating
sensor
failure board is 0 or
connection 3. If the temperature reads 0,
70

11-3
Troubleshooting

Optical 0x0100 The optical Optical system Damaged the temperature sensor is

assembl 0402 assembly temperature shorted. Replace related

y temperature sensor components

tempera reading at the 4. If the temperature reads 70,


Temperature Loose wire
ture analog drive the temperature sensor is open.
sensor
sensor board is 0 or First check for an unreliable
connection
failure 70 connection, then replace related
components
Diluent 0x0100 The optical Temperature Loose wire or
tempera 0403 assembly sensor damaged
ture temperature connection temperature
sensor reading at the sensor
failure analog drive
board is 0 or
70

preheat 0x0100 After startup, preheat Damaged Status 1. Enter the Status screen. If the
assembl 0404 the preheat assembly heater screen preheat bath temperature is less
y failure bath than 35 or greater than 55,
Damaged
temperature then there is an assembly failure
temperature
rises less than 2. If the problem persists after
sensor
1 degree per reconnecting the wires, replace
minute for Damaged the preheat assembly
consecutive 3 temperature
minutes switch

Assembly Loose wire


related
connections

Laser 0x0100 Laser current Optical system Damaged Voltage 1. First, check for an abnormal
failure 0809 is not within laser & Current laser current from the Status
[20, 70] Status screen
Laser drive Damaged
screen 2. Open the optical system
board board
shield box and turn the box
Optical system Damaged switch on and off manually. If
shield box

11-4
Troubleshooting

switch the laser is not illuminated,


check in turn if the connections
Optical system Loose wire
of optical system, box switch
related
and laser are reliable. If there is
connections
no connection problem, check if
the laser drive board and analog
drive board are working
properly according to the
hardware troubleshooting
procedures.

3. If the laser is illuminated


properly, then check if the laser
drive board is working properly
according to the hardware
troubleshooting procedures.
Otherwise, replace the optical
system.

4. If the laser is damaged,


replace the optical system

Syringe / Photocoupler Damaged System 1. Confirm the problem from


assembl photocoupler Self-test the System Self-test screen
y failure or dirty 2. Check the wires, the
surface photocoupler, the motor and
motion interference
Syringe motor Damaged
motor

Related Loose wire


connections

Drive Movement
mechanism limited by
mechanical
interference or
other causes

11-5
Troubleshooting

Sampli / Vertical / System 1. Confirm the problem from


ng photocoupler Self-test the System Self-test screen
assembl 2. Check the wires, the
Lifting motor /
y lifting photocoupler, the motor and
mechan Mechanical Motion motion interference
ism stop interference
failure
Related Loose wire
connections

Sampli / Horizontal / System 1. Confirm the problem from


ng photocoupler Self-test the System Self-test screen
assembl 2. Check the wires, the
Deflecting /
y photocoupler, the motor and
motor
swingin motion interference
g Mechanical Motion
mechan stop interference
ism
Related Loose wire
failure
connections

Backgr 0x0100 Background Reagent Reagent 1. Verify the reagent quality


ound 0702 unqualified at contaminated 2. If the background PLT is too
abnorm startup or expired high, verify the impedance
ality channel shield (please refer to
Analyzer tubes Abnormal
the treatment of impedance
shutdown
channel signal interference
Bad alarm)
maintenance
3. Verify the cleanness of the
practice
reaction bath and correct
maintenance practice

4. Check there is no leaks in


tube connections and valves

HGB 0x0100 HGB voltage WBC reaction There is Voltage 1. Check there is no foreign
blank in not within foreign matter & Current

11-6
Troubleshooting

voltage 0801 [3.2, 4.9] bath in the bath Status matter in the WBC bath
abnorm screen 2. Check the bath is correctly
The bath is
al filled with reagent
not filled with
diluent while 3. Verify the HGB blank voltage
measuring from the Voltage & Current
Status screen
Analyzer setup HGB gain
4. Verify the HGB gain settings
setup incorrect
5. Check the HGB assembly
HGB assembly Damaged
HGB
assembly

Fluid 0x0100 MCU reports Hydraulic Related tubes Status 1. Check if the fluid pressure is
pressure 0803 absolute sensor related folded screen close to current local
overloa pressure tubes Valve atmospheric pressure from the
d above 320 blockage or Status screen
kPa failure 2. Check there is no folding
tube, blocked or broken valve in
Optical system Severely
analyzer tubes
blocked
3. Check if there is obstruction
Hydraulic Loose in the sample probe or optical
sensor and connection or system
connection damaged
4. Check if there is loose
sensor
connection in hydraulic sensor
and its connecting wire

Abnor 0x0100 Vacuum is Waste pump Dirt blockage Status 1. Enter the Status screen.
mal 0804 not within the or failure screen Check if the pressure building
vacuum range process is normal (e.g. if the
specified by Related Dirt blockage waste pump is working
the sequence solenoids or failure properly)

2. Check if the pressure can be


Related tubes Leakage
maintained from the Status
screen. If not, check if there is
Analog drive Damaged gas leaking in related tubes or

11-7
Troubleshooting

board board valves;

3. If pressure building fails,


check if the waste pump fails or
if the tubes are leaking

4. If the waste pump is not


working properly, check if the
related connection and the
analog drive board is working
correctly

Abnor 0x0100 preheat bath Temperature Loose wire or Status 1. Check if the preheat bath
mal 0805 temperature sensor damaged screen temperature and the diluent
tempera out of range connection temperature temperature is as expected from
ture of [expected sensor the Status screen. Determine if
preheat temperature the abnormal status is caused by
bath -1.5, expected too great temperature difference
temperature during diluent replacement
+3] (recoverable, not a problem)

2. Solve the problem of the


sensor according to Temperature
Sensor Failure

preheat Refer to preheat Assembly Failure


assembly

High 0x0100 Optical Fan assembly Damaged fan Status 1. Check if the optical system
analyze 0806 temperature is or loose wire screen temperature is as expected from
r above 40 the Status screen
Analyzer air Air
tempera 2. Check if the fan assembly is
inlet/outlet inlet/outlet
ture working correctly (check for fan
blockage
assembly damage or analog
Optical system Loose wire or drive board damage), and if the
damaged air inlet/outlet is blocked
temperature
3. Solve the problem of the
sensor

11-8
Troubleshooting

log drive board Damaged sensor according to Temperature

board Sensor Failure

Diluent 0x0100 Diluent Fan assembly Damaged fan Status 1. Check if the diluent
tempera 080C temperature or loose wire screen temperature is as expected from
ture above 40 the Status screen
Ambient Room
above 2. Check if the fan assembly is
Temperature temperature
upper working correctly, and if the air
too high
limit inlet/outlet is blocked
Temperature Loose wire or
3. Solve the problem of the
sensor damaged
sensor according to Temperature
connection temperature
Sensor Failure
sensor
4. Check if the ambient
temperature is within the
specified operation temperature
range of the product

Diluent 0x0100 Diluent Ambient Temperature Status 1. Check if the diluent


tempera 080D temperature Temperature too low screen temperature is as expected from
ture below 10 the Status screen
Diluent Temperature
below 2. Solve the problem of the
temperature too low
lower sensor according to Temperature
limit Sensor Failure

3. Check if the ambient


temperature is within the
specified operation temperature
range of the product

Blocka 0x0100 Aperture RBC bath Aperture System /


ge 0901 voltage is too assembly blockage or Self-test
high (24V) or bad
changes connection
dramatically between
primary bath
and secondary

11-9
Troubleshooting

bath

Apertur 0x0100 Aperture Aperture Wrong System 1. Run aperture voltage test
e 0902 voltage below aperture Self-test from the System Self-test screen
voltage 17V specification to verify low aperture voltage
is too (temperature 2. If the aperture has been
Diluent Loose wire or
low corrected) replaced, check if there is wrong
temperature damaged
aperture specification,
sensor temperature
installation problem or cracked
assembly sensor
aperture

3. Troubleshoot the temperature


sensor according to Diluent
Temperature Sensor Failure

Impeda 0x0100 PLT noise RBC bath Loose shield / /


nce 0903 proportion assembly wire
channel over 10%
RBC bath Loose shield
signal
shield box box
interfer
ence Lab Unreliable
electromagneti ground
c environment connection

Analog drive Loose


board impedance
channel shield
box

Abnor 0x0100 Abnormal Flow cell Dirt blockage / 1. Problem 1: Clean the flow
mal 0904 sample cell
optical stream 2. Problem 2: Replace the
system optical system
signal

11-10
12 Debug
12.1 Mechanical Position Adjustment
Figure 1.1 Tooling Materials
Fixture Consumables
Operation Quality Control
Operation Content
Procedure QC Point QC Standard
Click on Service Sample Probe Debug. Enter the
1.1
Debug screen as shown in Figure 1.1.
First, click on the Initial position so that the sample Adjust the
1.2 probe is above the RBC bath, as shown in Figure 1.1. sample probe
position
Click on the WBC bath(or RBC bath) button from Fine tuning of The sample
the screen shown in Figure 1.1. Click the Middle the relative probe is
position button so that the probe wipe is shown. position basically in
Figure 1.2 Figure 1.3 Check if the sample probe is basically in the center of between the the center of
1.3 the bath. If there is any deviation, loosen the four sample probe the bath
screws shown in Figure 1.3 and slightly adjust the and the reaction
bath in left/right and front/back directions so that the bath
sample probe is in the center of the bath. Then
tighten one screw in each direction.
Adjustment screw for front and
back position
Adjust Note:
ment 1. In Figure 1.1, before clicking on Initialization, all the buttons except the Initialization button will be
screw
for left grey. Only after clicking on Initialization, the three buttons in the Sample probe horizontal position
and
right
column will be illuminated and available for use. The Middle position and Down position buttons will
positio not be available until clicking on the WBC bath or RBC bath button.
n

12-1
Debug

12.2 Commissioning of detection elements


12.2.1 Calibrate and verify the preheat temperature
Process Temperature Calibration
Figure 2.1
Tooling Fixture Materials

Operation Quality Control


Operation Content
Procedure QC Point QC Standard
Click the Start Menu bar, then
click Service Temp.&
Press. Calibration to enter the
Temperature calibration
2.1
screen. Click on the Preheat
bath temperature calibration
button to enter the screen
shown in Figure 2.1
Record the machine Measure the
measurement value, and enter preheat bath
Machine Fill in the sum of the the sum of the machine temperature
measurement value 2.2
machine measurement measurement value and the calibration
value and the FRU value
value on the service FRU as the factor
meter measurement value

12-2
Debug

12.2.2 Counting channel measurement


Figure 3.1 Crosshead
Tooling screwdriver Materials
Fixture

Operation Quality Control


Operation Content
Procedure QC Point QC Standard
Open the reaction bath shield box, run
3.1 several blank counts in the
WB-CBC+DIFF mode.
Ensure that there is no gas bubble in the Sample No gas bubble in the sample
sample tube and no fluid residue in the tube, tube, no fluid residue in the
sample probe during a complete count. sample sample probe, no gas in the
3.2 Otherwise, replace the sample tube. probe, DIFF sample supply tube and no
Check there is no gas in the sample supply bath bubble in the related tubes.
tube and no bubble in the WBC/RBC bath
related tubes.
Check if the fluid level of the WBC and WBC and There is no splash when WBC
RBC reaction bath is normal during the RBC or RBC bath is filled; bubbles
whole count process; if the probe tip is reaction can be correctly generated;
below the fluid level every time the probe bath bubbles do not contact with the
enters the bath; if there is any splash or filling tube; the probe tip is
3.3
bubble when adding fluids; if the bubbles below the fluid level every time
contact with the filling tube; if the WBC the probe enters the bath; the
and RBC bath can be drained correctly. WBC and RBC bath can be
correctly drained; no fluid
residue is on the bath wall.

12-3
Debug

Click ServiceSelf-test from the main Aperture Aperture voltage is normal and
menu bar to enter the Self-test screen, voltage no alarm is triggered.
as shown in Figure 3.1. Click on the
3.4
Aperture voltage button and record the
date generated in the Status Screen in the
record sheet
Installed the shield cover with M38 Tighten the
stainless steel panhead combination screws
3.5
screws and tighten the screws with a
crosshead screwdriver.
Run a count again and observe the sample The relative The relative position between
probe assembly during the whole process. position the sample probe and the
The sample probe shall not interfere with between reaction bath shield box
the shield box while the reaction bath is the sample
3.6
mixing. probe and
the reaction
bath shield
box
Note: The above-mentioned gas bubble refers to big bubble which separates the fluid in the tube.

12-4
13 Commissioning and Verification
After Servicing
Component Name Material ID Commissioning Items
Requirements After
Replacement
Pinaster board(5 051-001159-00 1. Perform data Version
ALL_AM1808) backup according to information is
the prompts correct
2. Check the
corresponding version
information from the
Version Info. screen
Analog Drive Board 115-018411-00 1. Perform HGB gain HGB blank
calibration voltage is 4.5V
2. Recalibrate the
MCV gain
Syringe transfer assembly 115-020624-00 Recalibration Verify the
10ml syringe 115-011902-00 reproducibility
Optical Sensor block of 3101-20-68304
injector
special bolt 3 041-005167-00
10ml syringe FRU 115-015338-00
250ul syringe(with 115-012708-00
nozzle)
Stepping motor 024-000366-00
Sampling Assembly 115-015676-00 1. Move the Verify the
Elevator 801-1805-00013- sampling assembly reproducibility
Motor(2S42Q-05640A) 00 horizontally and
Rotation Motor Position 801-3003-00015- vertically to ensure
Sensor Assembly 00 that the sample line is
sensor package 801-3001-00055- unobstructed with no
00 folding and

1.5 mop FRU 801-3102-00057- interference with the

13-1
Commissioning and Verification After Servicing

00 pre-amplifier, fluidics

Sample Probe(for 801-3101-00002- separator, right door,

BC-5300 only) 00 motor, valves and line


straps
2. Ensure the sample
line is not squeezed or
deformed at the line
straps
3. Ensure there is no
folding or interference
when the wipe fluid
line is moving
horizontally or
vertically in the
sample assembly
4. Mechanical
commissioning of the
relative position
between the sample
probe and the
WBC/RBC bath
5. Recalibration
RBC cound pool 115-018412-00 1. Mechanical Verify the
module(FRU) commissioning of the reproducibility
relative position
between the sample
probe and the bath
2. The waste line for
the reaction bath needs
to be wrapped in the
vertical direction to a
height above the liquid
level with 3mL of
liquid in the bath
3. Perform HGB gain

13-2
Commissioning and Verification After Servicing

calibration
4. Recalibration
HGB cound pool module 115-015987-00 1. Mechanical 1. Verify the
commissioning of the reproducibility
relative position 2. HGB blank
between the sample voltage is 4.5V
probe and the bath
2. The waste line for
the reaction bath needs
to be wrapped in the
vertical direction to a
height above the liquid
level with 3mL of
liquid in the bath
3. Perform HGB gain
calibration
Front bath washer 801-3005-00057- 1. Install the aperture 1. Verify the
(molding WH02-205) 00 so that the surface with reproducibility
Microsensor (D50m) 045-000809-00 the tapered bore faces 2. Verify the
the primary bath aperture voltage
2. Recalibration
RBC shielding bottom 042-007064-00 Mechanical position Verify that the
plate welding piece adjustment of RBC sampling assembly
bath will not contact
with the RBC bath
when moving
vertically in the
RBC bath position
HGB FRU 115-018415-00 Perform HGB gain HGB blank
calibration voltage is 4.5V
Vacuum unit 115-015673-00 / Verify that the
Waste pump assembly 115-018416-00 reaction baths can
(FRU) be correctly
drained and
vacuum can be

13-3
Commissioning and Verification After Servicing

correctly built
2-way Valve (Mindray) 801-3201-00002- / 1. Verify the
00 orientation of the
2-way Valve (Mindray) 801-3201-00004- inlet/outlet is
00 correct;
2. The hose shall
be fully inserted;
3. Thick 50 tubes
can not be used
again after
disconnected from
valve ports or
connectors
3-way Valve (Mindray) 801-3201-00003- / 1. Verify the NO,
00 NC and Common
terminals are
connected
correctly;
2. The hose shall
be fully inserted;
3. Thick 50 tubes
can not be used
again after
disconnected from
valve ports or
connectors
LVM valve assembly 115-015675-00 Recalibration 1. Verify the NO,
NC and Common
terminals are
connected
correctly;
2. The hose shall
be fully inserted;
3. Verify the
reproducibility

13-4
Commissioning and Verification After Servicing

1ml Pump(eject) 115-014601-00 Recalibration /


200ul pump 115-014598-00 Recalibration /
TAKASAGO value assem 115-014726-00 / 1. Verify the
pinch valve
assembly (T34) is
properly positioned
in the valve;
2. Cut T75 and
case it between
T34 and C11
BC-5000 front cover 043-002705-00 Perform touchscreen Verify normal
BC-5150 front cover 043-002706-00 calibration operations of the
Touch Panel 801-3110-00184- touchscreen
00
LCD Module(5000) 115-018417-00
LCD Module(5150) 115-018418-00
Reagent preheating unit 115-015672-00 1. J9 and C10 must Verify the
be full inserted temperatures are
2. FRU record within the ranges
parameters enters new from the Status
temperature value screen
from the Temperature
Calibration screen
Battery Lithium 3V 801-3100-00226- Reset date and time After startup, the
35mAh D12.5*2.0 00 in the Setup screen. date remains the
Save and reset the same
analyzer
Fan assembly 115-017923-00 / Verify if the fan
rotates
Secure Digital Memory 023-000549-00 1. Backup the data /
Card 8GB before replacing the
SD card (if possible);
2. Restore the
configuration data;
exoteric sampling 043-002703-00 Adjust the 2 M3 hex 1. Verify there is

13-5
Commissioning and Verification After Servicing

keystoke screws which fasten a uniform gap


Start key for open-vial 801-3100-00194- the aspirate key, in between the key
mode 00 order to achieve a and the front cover
uniform gap between 2. Key operation
the key and the front is normal
cover
tube package 115-017475-00 / See the Fluidic
System Precautions
for Assembly and
Service section in
the Service Manual
connector package 115-017476-00 / See the Fluidic
System Precautions
for Assembly and
Service section in
the Service Manual

13-6
14 Service BOM

Part N.O. Part Name

051-001159-00 Pinaster board (5 ALL_AM1808)

115-018411-00 Analog Drive Board

051-001319-00 3107 Power board PCBA

051-001062-00 Indicator board PCBA

051-000881-00 6301 touch control board PCBA

115-020624-00 Syringe transfer assembly

115-011902-00 10ml syringe

3101-20-68304 Optical Sensor block of injector

041-005167-00 special bolt 3

115-015338-00 10ml syringe FRU

115-012708-00 250ul syringe(with nozzle)

024-000366-00 Stepping motor

115-015676-00 Sampling Assembly

801-1805-00013-00 Elevator Motor(2S42Q-05640A)

801-3003-00015-00 Rotation Motor Position Sensor Assembly

042-007187-00 probe press plate

801-3001-00055-00 sensor package

801-3102-00057-00 1.5 mop FRU

801-3101-00002-00 Sample Probe(for BC-5300 only)

3005-20-44746 probe wipe clamp

115-018412-00 RBC count pool module(FRU)

042-007065-00 RBC shielding box

115-015987-00 HGB count pool module


Service BOM

043-000711-00 filter

801-3003-00045-00 Isolation chamber clamp

801-3005-00057-00 Preventive Kit

045-000809-00 Ruby Red Cell Counter(D50um)

M6M-010063--- Seal for pressure sampling connector

042-007064-00 RBC shielding bottom plate welding piece

115-018414-00 RBC pool cover

115-018415-00 HGB FRU

115-015673-00 Vacuum unit

115-018416-00 waste water pump assembly

801-1805-00006-00 Vacuum chamber assembly

801-3201-00002-00 2-way Valve (Mindray)

801-3201-00003-00 3-way Valve (Mindray)

801-3201-00004-00 2-way Valve (Mindray)

115-015675-00 LVM valve assembly

115-014601-00 1ml Pump(eject)

115-014598-00 200ul pump

115-014726-00 TAKASAGO value assem

043-002705-00 BC-5000 front cover

043-002706-00 BC-5150 front cover

801-3110-00184-00 Touch Panel, resistive, 10.4, four wire

115-018417-00 LCD Module(5000)

115-018418-00 LCD Module (5150)

115-015672-00 Reagent preheating unit

115-015678-00 Micro-switch assembly

M07-00131F--- FUSE Time-lag 250V 3.15AD5X20

0030-10-13055 Power filter

14-2
Service BOM

115-015677-00 Diluent temperature testing unit

115-017730-00 liquid detect assembly

801-3100-00226-00 Battery Lithium 3V 35mAh D12.5*2.0

115-017923-00 Fan assembly

509B-10-05996 Chinese power cord

801-3201-00053-00 M-68D CAP Assembly

115-016034-00 DIFF Lyse Cap Assembly

115-013091-00 Waste bottle cap assembly

801-3110-00167-00 Diluent Assembly holder

023-000549-00 Secure Digital Memory Card 8GB

110-002557-00 Installer CD

043-002703-00 exoteric sampling keystoke

801-3100-00194-00 Start key for open-vial mode

115-017475-00 tube package

115-017476-00 connector package

051-001621-00 Liquid detect board PCBA

043-000829-00 Reagent detection tube

14-3
15 Appendices
A. Fluidic diagram

A-1
B. Connection and Tube
Position
Name in
Material in the
ID the Part NO. Name
type Fluidic
diagram
diagram
1 syringe SR 115-015652-00 syringe assembly D2
2 WBC WBC 115-015987-00 GHB cound pool module B3
3 RBC RBC 115-015986-00 RBC cound pool module B4
4 Vacuum VC 115-015673-00 Vacuum/Pressure Chamber D5
5 Pump LP 115-015674-00 waste water pump subassembly D4
6 probe SPB 3101-20-68488 sampling probe D2
7 probe wipe SPB 3102-20-69178 probe wipe D2
Isolation
chamber1,I
chamber 115-002439-00 Isolation chamber filter C3,C4
solation
8 chamber2
9 Valve SV1 115-010088-00 3-way Valve (Mindray) A4
10 Valve SV2 115-010088-00 3-way Valve (Mindray) A4
mirco solenoid valve of two-way(higher
Valve SV3 115-007667-00 C2
11 operating pressure)
12 Valve SV4 115-010089-00 2-way Valve (Mindray) B2
13 Valve SV5 115-010089-00 2-way Valve (Mindray) C2
14 Valve SV6 115-010089-00 2-way Valve (Mindray) A2
15 Valve SV7 115-010089-00 2-way Valve (Mindray) B5
16 Valve SV8 115-010088-00 3-way Valve (Mindray) A4
17 Valve SV9 115-010089-00 2-way Valve (Mindray) D3
18 Valve SV10 115-010089-00 2-way Valve (Mindray) C3
19 Valve SV11 082-000446-00 Valve.3-way -75~250kPa DC12V C2
20 Valve SV12 115-010089-00 2-way Valve (Mindray) B5
21 Valve SV13 115-010089-00 2-way Valve (Mindray) C5
22 Valve SV14 115-010089-00 2-way Valve (Mindray) C3
23 Valve SV15 115-010089-00 2-way Valve (Mindray) C3
24 Valve SV16 115-010089-00 2-way Valve (Mindray) C2
25 valVe PV18 115-014726-00 TAKASAGO value assem B3
Hydraulic
Sensor 115-017730-00 liquid detect assembly
26 Sensor C2
Preheat
Preheat Bath 115-015672-00 Preheat Bath A3
27 Bath
28 Pump DP1 115-014601-00 1ml Pump(eject) A4
29 Pump DP2 115-014598-00 200ul pump B4
30 Valve CV1 BA40-10-62038 Check Valve A of WU A3
Temperatur
31 Sensor e Sensor 115-015677-00 Diluent temperature testing unit B1
32 Filter LF 0010-10-12408 Inline Filter 43um 1/8 I.D. Tubing A5
33 / / / / /
34 / / / / /
35 Tube T1 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 C1
36 Tube T2 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 C1
37 Tube T3 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 B1
38 Tube T4 0040-10-32301 Tubing. FEP,0.040"X0.066"1feet D2
39 Tube T5 M90-100031--- tube.PTFE,1.7mmIDX2.55mmOD C2
40 Tube T6 M90-100031--- tube.PTFE,1.7mmIDX2.55mmOD C2
41 Tube T7 M90-100031--- tube.PTFE,1.7mmIDX2.55mmOD C2
42 Tube T8 M6G-020007--- Tube OD3mm ID1mm EVA C2
43 Tube T9 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 C1
44 Tube T10 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 C1
45 Tube T11 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 C1
46 Tube T12 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 C2
47 Tube T13 M90-100071--- Tubing.3/32"X5/32",S-50-HL AAX02004,Tygon(20feet)
C2

B-1
Appendices

Position
Name in
Material in the
ID the Part NO. Material description
type Fluidic
diagram
diagram
48 Tube T14 M90-100071--- Tubing.3/32"X5/32",S-50-HL AAX02004,Tygon(20feet)
C3
49 Tube T15 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon C3
50 Tube T16 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon C4
51 Tube T17 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 A1
52 Tube T18 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 B1
53 Tube T19 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 A4
54 Tube T20 M90-000025--- Tube.1/8"X1/4",R-3603 AAC02007,Tygon A5
55 Tube T21 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 A2
56 Tube T22 M90-100031--- Tube.PTFE,0.066"IDX0.098"OD C2
57 Tube T23 M90-100031--- Tube.PTFE,0.066"IDX0.098"OD B2
58 Tube T24 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 B5
59 Tube T25 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran A4
60 Tube T26 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran A4
61 Tube T27 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 B3
62 Tube T28 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 B3
63 Tube T29 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 B4
64 Tube T30 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran A4
65 Tube T31 M90-100031--- Tube.PTFE,0.066"IDX0.098"OD A3
66 Tube T32 M90-100031--- Tube.PTFE,0.066"IDX0.098"OD A3
67 Tube T33 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD B3
68 Tube T34 M6G-020034--- 3-way valve tubing(1.6*3.2 B3
69 Tube T35 0040-10-32301 Tube.PTFE,0.040"IDX0.066"OD B3
70 Tube T36 3001-10-07069 Tube.1/16"X1/8",S-50-HLAAX02002,Tygon C3

71 Tube T37 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon C4


72 Tube T38 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon C4

73 Tube T39 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon C4


74 Tube T40 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon C5
75 Tube T41 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon C5
76 Tube T42 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon C5
77 Tube T43 3001-10-07069 Tube.1/16"X1/8",S-50-HLAAX02002,Tygon C4
78 Tube T44 3001-10-07069 Tube.1/16"X1/8",S-50-HLAAX02002,Tygon D3
79 Tube T45 0040-10-32301 Tube.PTFE,0.040"IDX0.066"OD B2
80 Tube T46 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon C3
81 Tube T47 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran A4
82 Tube T48 M90-100031--- Tube.PTFE,0.066"IDX0.098"OD A4
83 Tube T49 M6G-020056--- Tube.EVA,ID:1/16,OD1/8clear A4
84 Tube T50 M6G-020056--- Tube.EVA,ID:1/16,OD1/8clear A5
85 Tube T51 3001-10-07069 Tube.1/16"X1/8",S-50-HLAAX02002,Tygon B5
86 Tube T52 3001-10-07069 Tube.1/16"X1/8",S-50-HLAAX02002,Tygon B4
87 Tube T53 082-000108-00 Tube.M-87-D3,2mmX3.5mm,AV31X2103 C2
88 Tube T54 3001-10-07069 Tube.1/16"X1/8",S-50-HLAAX02002,Tygon B5
89 Tube T55 3001-10-07069 Tube.1/16"X1/8",S-50-HLAAX02002,Tygon C5
90 Tube T56 M90-000025--- Tube.1/8"X1/4",R-3603 AAC02007,Tygon C5
91 Tube T57 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon C2
92 Tube T58 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon C4
93 Tube T59 M90-100031--- Tube.PTFE,0.066"IDX0.098"OD B3
94 Tube T60 M90-100031--- Tube.PTFE,0.066"IDX0.098"OD B4
95 Tube T61 M6G-020006--- Tube.Silicone,1/16"X3/16",TYGON 3350 B3
96 Tube T62 M6G-020006--- Tube.Silicone,1/16"X3/16",TYGON 3350 B4

B-2
Appendices

Name in Position
Material
ID the Part NO. Material description in the
type
diagram Fluidic
97 Tube T63 0040-10-32301 Tube.PTFE,0.040"IDX0.066"OD B3
98 Tube T64 M90-100031--- Tube.PTFE,0.066"IDX0.098"OD B2
99 Tube T65 0040-10-32301 Tube.PTFE,0.040"IDX0.066"OD B2
100 Tube T66 M90-000025--- Tube.1/8"X1/4",R-3603 AAC02007,Tygon C5
101 Tube T67 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon C5
102 Tube T68 M90-000025--- Tube.1/8"X1/4",R-3603 AAC02007,Tygon D5
103 Tube T69 M90-000025--- Tube.1/8"X1/4",R-3603 AAC02007,Tygon D4
104 Tube T70 M6G-020034--- 3-way valve tubing(1.6*3.2) C5
105 Tube T71 M6G-020034--- 3-way valve tubing(1.6*3.2) B5
106 Tube T72 M6G-020055--- Tube.TPU,ID1/8,OD1/4,clear B5
107 Tube T73 M90-000025--- Tube.1/8"X1/4",R-3603 AAC02007,Tygon D5
108 Tube T74 M6G-020055--- Tube.TPU,ID1/8,OD1/4,clear D4
109 Tube T75 M90-000025--- Tube.1/8"X1/4",R-3603 AAC02007,Tygon B3
110 Tube T76 M90-000025--- Tube.1/8"X1/4",R-3603 AAC02007,Tygon A4
111 Tube T77 M6G-020006--- Tube.Silicone,1/16"X3/16",TYGON 3350 B2
112 Tube T78 0040-10-32301 Tube.PTFE,0.040"IDX0.066"OD B2
113 Tube T79 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon B2
114 Transit tube J1 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD1feet D2
115 Transit tube J2 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD1feet C2
116 Transit tube J3 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran C2
117 Transit tube J4 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran C2
118 Transit tube J5 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran C2
119 Transit tube J6 M6G-020006--- Tube.Silicone,1/16"X3/16",TYGON 3350 C2
120 Transit tube J7 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran A3
121 Transit tube J8 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran A3
122 Transit tube J9 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran B3
123 Transit tube J10 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD1feet B3
124 Transit tube J11 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD1feet B3
125 Transit tube J12 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD1feet B2
126 Transit tube J13 0030-20-13339 Transit tube(1.5mm-3mm)(Mould MR13339) B2
127 Transit tube J14 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran A4
128 Transit tube J15 0030-20-13339 Transit tube(1.5mm-3mm)(Mould MR13339) A4
129 Transit tube J16 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon B3
130 Transit tube J17 M6G-020006--- Tube.Silicone,1/16"X3/16",TYGON 3350 B3
131 Transit tube J18 M90-100071--- Tube.3/32"X5/32",S-50-HLAAX02004,Tygon B4
132 Transit tube J19 M6G-020006--- Tube.Silicone,1/16"X3/16",TYGON 3350 B4
133 Transit tube J20 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD1feet B2
134 Transit tube J21 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD1feet B3
135 Transit tube J22 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran B2
136 Transit tube J23 0030-20-13339 Transit tube(1.5mm-3mm)(MouldMR13339) B2
137 Transit tube J24 M6G-020009--- Tubing. Silica gel 0.031"ID 0.156"OD1feet B2
138 Transit tube J25 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran B2
139 Transit tube J26 0030-20-13339 Transit tube(1.5mm-3mm)(MouldMR13339) B2
140 Transit tube J27 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran C2
141 Transit tube J28 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran C2
142 Transit tube J29 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran A3
143 Transit tube J30 0030-20-13339 Transit tube(1.5mm-3mm)(MouldMR13339) B2
144 Transit tube J31 0030-20-13339 Transit tube(1.5mm-3mm)(MouldMR13339) B2

B-3
Appendices

Name
Position
in
Material in the
ID the Part NO. Material description
type fluidic
diagr
diagram
am
145 Connection C1 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 C1
146 Connection C2 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 C1
147 Connection C3 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 C1
148 Connection C4 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 C1
149 Connection C5 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 C3
150 Connection C6 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 B5
151 Connection C7 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 C5
152 Connection C8 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 C5
153 Connection C9 M90-100028--- 3/32 PE TEE FITTING-WHITE NYL T420-1 C4
154 Connection C10 043-000880-00 8.5mm three way connector B3
155 Connection C11 M90-100028-03 Connection.Y,400Barb,3/32"ID,White Nylon B3
156 Connection C12 M90-100026--- Nylon plug (located at the reagent inlet) B3
157 Connection C13 M90-100026--- Nylon plug (located at the reagent inlet) B4
158 Connection C14 043-000892-00 straight tube tie-in A4
159 Connection C15 043-000892-00 straight tube tie-in A4
160 Connection C16 M90-100027--- Connection.Straight Through Reduction,1/8"&3/32"ID C5
161 Connection C17 M90-100027--- Connection.Straight Through Reduction,1/8"&3/32"ID A5
162 Connection C18 082-001140-00 Connection.Male Luer 2mm"ID natural PVDF B5
163 Connection C19 082-001141-00 Connection.FemaleLuer,1/4-28UNF,1/8"ID B5
164 Connection C20 082-001140-00 Connection.Male Luer 2mm"ID natural PVDF B5
165 Connection C21 082-001141-00 Connection.FemaleLuer,1/4-28UNF,1/8"ID B5
166 Connection C22 M90-100009--- Connection.FemaleLuer,1/4-28UNF,1/8"ID A5
167 Connection C23 M90-100025--- Connection.Male Luer,1/8"Barb,White Nylon A5
168 Connection C24 M90-100009--- Connection.FemaleLuer,1/4-28UNF,1/8"ID B4
169 Connection C25 M90-100025--- Connection.Male Luer,1/8"Barb,White Nylon B4
170 Connection C26 M90-100100--- Connection.Elbow,400Barb,3/32"ID,White,L420-1 C2
171 Connection C27 M90-100100--- Connection.Elbow,400Barb,3/32"ID,White,L420-1 A2
172 Tube T80 0040-10-32301 Tube.PTFE,0.040"IDX0.066"OD A4
173 Transit tube J32 0030-20-13339 Transit tube(1.5mm-3mm)(Mould MR13339) A4
174 Transit tube J33 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran C2
175 Transit tube J34 082-000055-00 Tube.1/16"X3/16",F-5500-A,Fluran C2
176 Tube T81 M6G-020056--- Tube.EVA,ID:1/16,OD1/8clear B5
177 Tube T82 M6G-020056--- Tube.EVA,ID:1/16,OD1/8clear A5

B-4
C. Hardware block diagram

C-1
D. Cables and Wires
Material ID Name Connection
009-002857-00 Heater connecting wire Connects A-J21 with heater and
temperature switch
009-002858-00 Temperature sensor Connects A-J10 with temperature
connecting wire sensor
009-002863-00 Indicator board connecting Connects B-J78 with indicator board
wire
009-002865-00 Analog board supervisory Connects A-J2 and B-J86
signal wire
009-002866-00 Optical signal output wire Connects A-J4 and B-J85
009-002867-00 Analog main control board Connects A-J31 and B-J81
SPI signal wire
009-002869-00 Optical control signal wire Connects A-J3 and B-J77
009-002870-00 Syringe motor connecting Connects A-J32 and syringe motor
wire
009-002871-00 Digital power connecting Connects C-J1/C-J2 and A-J22/B-J68
wire
009-002872-00 Analog power connecting Connects A-J8 and C-J3 C-J4
wire
009-002875-00 Sampling motor connecting Connects A-J24 and sampling motor
wire
009-002877-00 Electromagnetic metering Connects A-J16 and electromagnetic
pump connecting wire metering pump
009-002878-00 Valve connecting wire Connects A-J14 A-J15 and valves
009-002880-00 Waste pump connecting Connects A-J20 and waste pump
wire
009-002881-00 Float switch connecting Connects A-J1 and float switch
wire
009-002882-00 Sampling photocoupler Connects A-J12 and sampling
connecting wire photocoupler
009-002911-00 Touchscreen control cable Connects B-J67 and touchscreen drive
board
009-002913-00 LCD signal wire Connects B-J4/B-J16 and LCD/backlight
009-002967-00 RBC connecting wire Connects A-J6 and RBC bath
009-002988-00 Safety ground /
009-002989-00 Component ground /
009-003176-00 Syringe assembly Connects A-J13 and syringe assembly
photocoupler connecting photocoupler
wire
009-003322-00 Start key connecting wire Connects B-J99 and start key
009-003381-00 Fan adapter cable Connects A-J17 A-J18 and fan
009-003642-00 Hydraulic sensor adapter Connects A-J11 and hydraulic sensor
cable
2800-20-28762 HGB connecting wire Connects A-J5 and HGB bath
009-004057-00 LH&DIFF detect board wire Connects A-J26 and Liquid detect
board PCBA
Note: A refers to the analog drive board, B refers to the main control board, C refers to the power
board
E. Appendix Table
Product Model SERIAL NUMBER
No. Inspection Item Description Requirement Result Conclusion
Refer to related
Power cable
1 Electrical connection graphic files in OK NG PASS FAIL
connection
the appendix
Red indicates
2 Indicator color Color problems, green OK NG PASS FAIL
indicates OK
Alarms when
3 Indicator sound Sound OK NG PASS FAIL
problems exist
Touchscreen
4 Touchscreen Touchscreen calibration OK NG PASS FAIL
completed
Correct time and
5 Time and date Enter time and date OK NG PASS FAIL
date entry
Version and
6 Version configuration In latest versions OK NG PASS FAIL
information
Syringe and sampling Syringe and
7 mechanism self-test sampling Normal operation OK NG PASS FAIL
mechanism self-test

8 Valves self-test Valves Normal operation OK NG PASS FAIL

9 Fan self-test Fan Normal operation OK NG PASS FAIL


Adjustment of the
Refer to
relative position
Mechanical position Mechanical
10 between the OK NG PASS FAIL
adjustment position
sample probe and
adjustment
the WBC bath
Adjustment of the
Refer to
relative position
Mechanical position Mechanical
11 between the OK NG PASS FAIL
adjustment position
sample probe and
adjustment
the RBC bath
Pressure within
12 Pressure verification Vacuum OK NG PASS FAIL
range
Preheat bath Machine
Temperature
13 temperature measurement PASS FAIL
calibration
calibration value
Preheat bath
Temperature
14 temperature FRU value PASS FAIL
calibration
calibration
Preheat bath
Temperature
15 temperature Overall difference PASS FAIL
calibration
calibration
Counting channel Bubbles in sample
16 No bubbles OK NG PASS FAIL
measurement tube or not
Counting channel Fluid residue on the
17 No residue OK NG PASS FAIL
measurement sample probe
Counting channel Sample probe
measurement below the fluid level Normal down
18 OK NG PASS FAIL
and clear from the position
reaction bath
Counting channel Bubbles in sample
19 No bubbles OK NG PASS FAIL
measurement supply tube or not
Counting channel Bubbles in WBC
20 Bubbles in bath OK NG PASS FAIL
measurement bath or not
Counting channel Bubbles in RBC bath
21 Bubbles in bath OK NG PASS FAIL
measurement or not

E-1
Connection and Tube

Counting channel
22 Aperture voltage [16, 21]V PASS FAIL
measurement
Counting channel splash when WBC
measurement bubbling or not, No splash and no
23 OK NG PASS FAIL
bubbles on filling contact
tube or not
Counting channel splash when RBC
measurement bubbling or not, No splash and no
24 OK NG PASS FAIL
bubbles on filling contact
tube or not
Counting channel WBC bath can be
25 measurement drained correctly or Drained correctly OK NG PASS FAIL
not
Counting channel RBC bath can be
26 measurement drained correctly or Drained correctly OK NG PASS FAIL
not
Counting channel
27 WBC bath wall No residue OK NG PASS FAIL
measurement
Counting channel
28 RBC bath wall No residue OK NG PASS FAIL
measurement
Counting channel RBC bath shield box
29 Screws tightened OK NG PASS FAIL
measurement installation
Sample probe
Counting channel Sample probe
30 moves with no OK NG PASS FAIL
measurement movement
interference
Sample probe
Counting channel tube does not
31 Sample probe tube OK NG PASS FAIL
measurement interfere with
other structures
Perform
Refer to the user's
32 Maintenance maintenance and OK NG PASS FAIL
manual
cleaning
Reaction bath voltage
RBC aperture
33 measurement and [16, 21]V PASS FAIL
voltage
setup
Analyzer status
34 RBC 0.021012/L PASS FAIL
verification - count
Analyzer status
35 HGB 1g/L PASS FAIL
verification - count
Analyzer status
36 HCT 0.5% PASS FAIL
verification - count
Analyzer status
37 PLT 10109/L PASS FAIL
verification - count
Connect the optical Refer to the
Optical system tube
38 tubes and fill with service OK NG PASS FAIL
connection
fluids instructions

39 Analyzer voltage Power (+12V) [11.5, 12.5]V PASS FAIL


Analyzer voltage Power (+24V) [22.0, 29.0]V PASS FAIL
40
Analyzer voltage Analog (+12V) [11.5, 12.5]V PASS FAIL
41
Analyzer voltage Constant current
42 source voltage [47.0, 60.0]V PASS FAIL
(directly measured)
Analyzer voltage LAS blank voltage [0, 0.5]V PASS FAIL
43

44 Analyzer current Laser current [20, 70]mA PASS FAIL

45 Background Count WBC 0.2109/L PASS FAIL

46 Background Count RBC 0.021012/L PASS FAIL


Background Count HGB 1g/L PASS FAIL
47

E-2
Connection and Tube

Background Count HCT 0.5% PASS FAIL


48
Background Count PLT 10109/L PASS FAIL
49
Standard particle Total LAS 1500~3000
50 PASS FAIL
graphic parameter test
Standard particle LAS CG Position 38~42
51 PASS FAIL
graphic parameter test
Standard particle LAS CV 6.50%
52 PASS FAIL
graphic parameter test
Standard particle Total MAS 1500~3000
53 PASS FAIL
graphic parameter test
Standard particle MAS CG Position 107~158
54 PASS FAIL
graphic parameter test
Standard particle MAS CV 3.0%
55 PASS FAIL
graphic parameter test
Standard particle Total WAS 1500~3000
56 PASS FAIL
graphic parameter test
Standard particle WAS CG Position 135~220
57 PASS FAIL
graphic parameter test
Standard particle WAS CV 8.0%
58 PASS FAIL
graphic parameter test
Optical gain N/A: Not
59 LAS PASS FAIL
applicable
Optical gain N/A: Not
60 MAS PASS FAIL
applicable
Optical gain N/A: Not
61 WAS PASS FAIL
applicable
Optical gain N/A: Not
62 Width PASS FAIL
applicable
Impedance gain N/A: Not
63 RBC PASS FAIL
applicable
HGB gain N/A: Not
64 HGB PASS FAIL
applicable
No failure or
65 Shutdown Shutdown process OK NG PASS FAIL
alarm occurs

Tested by: Date:

E-3
P/N: 046-008515-00(3.0)