Carbohydrate Identification Lab Report-Abby Lacy

PROCEDURE:
We first conducted the Benedict’s solution test which identifies reducing sugars. We
were able to find that one of the non-reducing sugars was in test tube D because it did not react
in any way to the solution. The quality that helped us identify the reducing sugars was the
orange color in the precipitate. The reducing sugars consisted of test tubes A, B, and C. We then
did the iodine test second because it revealed a quality of a blue/black/purple color which allows
us to identify it as starch which was test tube E, which we are then able to eliminate from further
testing, since it is the only carbohydrate that will react with the solution. Since A, B, and C were
identified as the reducing sugars and E was identified as starch, that left sucrose to be identified
as test tube D. We then chose to conduct the Barfoed’s solution test in order to identify more of
the carbohydrates. The indication of the reaction is if the dissolved carbohydrate reacts after
exactly two minutes by creating a brick red-colored precipitate. If this precipitate appears after 2
minutes, the dissolved carbohydrate is a monosaccharide. As identified earlier, the reducing
sugars were in test tubes A, B, and C which are lactose, glucose/aldose, and fructose/ketose
respectively. Lactose is not a monosaccaride, so we were able to identify it as test tube A, as it
also did not produce the brick red-colored precipitate. Lastly, we conducted the Seliwanoff test
which identified ketose which was fructose as test tube B, due to it reacting by turning the red
color much faster than test tube C, which identifies as the aldose which was glucose as it took a
lot longer to react.

Results:

Test Tubes #32154
A-Lactose
B-Glucose
C-Fructose
D-Sucrose
E-Starch

Test Tube Benedict’s Iodine Test Barfoed Test Seliwanoff Inferences
Test Test
A Reducing Didn’t test Not a N/A Lactose
Sugar- because monosaccharide-
Turned starch is not no precipitate
Orange a reducing
sugar and
this test was
used to
discover
starch

and more specifically. so that told us that test tube E was starch. . fructose. We then proceeded to test D and E and neither of them had any signs of a reaction or change. B. we tested Seliwanoff to find out if B or C was ketose. but it came back with no reaction. and the only result we saw was an orange color from the iodine. because Red precipitate no Turned starch is not at the bottom precipitate Orange a reducing appeared sugar and this test was used to discover starch C Reducing Didn’t test Monosaccharide. Glucose Sugar. We tested B first. leading us to the conclusion that it was ketose. Next. Lastly. We tested test tube A. Stayed the N/A N/A Sucrose Reducing same color Sugar E Non. and C came back with positive results as the color changed to orange when tested. B Reducing Didn’t test Monosaccharide. Starch. test tubes A. After that. N/A N/A Starch Reducing turned dark Sugar blue/black or purple color WRITTEN RESULTS: When testing the Benedict’s solution. indicating that they are monosaccharides. we tested Barfoed’s solution. We then proceeded to test B and C and the results came back positive with a red precipitate at the bottom. telling us that it was not a monosaccharide. there was no precipitate. B. When we tested E. but the carbohydrate did not react or in other words. but no reaction. We tested D. we tested iodine to discover the starch. or C since we already knew they were reducing sugars and that the starch had to either be in test tube D or E. and a precipitate appeared.Ketose. because Red precipitate precipitate Turned starch is not at the bottom appeared Orange a reducing sugar and this test was used to discover starch D Non. we were able to see a dark purple color after putting the iodine in. This enabled us to identify them as the reducing sugars. so we knew they were the non-reducing sugars. so we did not test A. Fructose Sugar. but then tested C.Not ketose.

and most importantly: Fructose. and is lactose specifically. disaccharide. . but had a precipitate at the bottom after Barfoed’s test. ketose. and is glucose specifically. I think that test tube C is the a monosaccharide. monosaccharide. After testing test tube D for the Benedict’s solution and getting a negative result (the color did not change/no reaction occurred). so I made an inference that it was starch. this leads me to believe that it is a reducing sugar. it was the only one to turn purple. Since test tube B also turned orange in the Benedict’s.CONCLUSION: Because test tube A turned orange in the Benedict’s test and did not have a precipitate at the bottom after Barfoed’s test. Lastly. after testing test tube E in the iodine test. and did not have a precipitate after the Seliwanoff test. Test tube C also turned orange after conducting the use of Benedict’s solution along with having the precipitate appear at the bottom after the Barfoed’s test as well as having the precipitate appear at the bottom after the Seliwanoff test. and also staying the same color during the iodine test lead me to think that test tube D was the sucrose. I believe test tube B is also a reducing sugar.