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Cladosporium carrionii, is an agent of chromoblastomycosis in subtropical and tropical regions

(grows at 35-37C).
The species Cladosporium carrionii is found to be heterogeneous.

Cladophialophora carrionii
Synonym: Cladosporium carrionii

Cladophialophora carrionii is a recognised agent of chromoblastomycosis and it has been isolated

from soil and fence posts made from Eucalyptus spp. Cases of chromoblastomycosis caused by C.
carrionii are commonly found in Australia, Venezuela, Madagascar and South America. Isolates
from phaeomycotic cysts and opportunistic infections have also been reported.

RG-2 organism.

Morphological Description: Colonies are slow growing, reaching 3-4 cm in diameter after one
month, with a compact suede-like to downy surface and are olivaceous-black in colour. Microscopy
shows ascending to erect, olivaceous-green, apically branched, elongate conidiophores producing
branched acropetal chains of conidia. Conidia are pale olivaceous, smooth-walled or slightly
verrucose, limoniform to fusiform, 1.5-3.0 x 2.0-7.0 m in size. Bulbous phialides with large
collarettes and minute, hyaline conidia are occasionally formed on nutritionally poor media.
Maximum growth temperature 35-37C.

Molecular Identification: ITS sequencing is recommended (Abliz et al. 2004; de Hoog et al.

Key Features: Conidia are smaller and comprise heavily branched chains which fall apart much
more easily than in the other Cladophialophora species.

References: McGinnis (1980), Rippon (1988), de Hoog et al. (1995, 2000, 2015).

Culture of Cladophialophora carrionii.

Conidiophores and conidia of C. carrionii.

Cladophialophora carrionii is one of the relatively common causative agents of chromoblastomycosis.

We have developed the specific oligonucleotide primer set based on the internal transcribed spacer
regions of ribosomal DNA for the rapid identification of this pathogen. PCR with this primer set
amplified a 362-bp amplicon from C. carrionii strains. From other relevant dematiaceous species,
including medically important dematiaceous fungi, such as Fonsecaea pedrosoi, Phialophora
verrucosa, and Exophiala dermatitidis, and eight species of medically important yeasts, such as
Candida albicans and Cryptococcus neoformans var. neoformans, the primer set did not produce any
amplicon. PCR with this primer set may be a useful tool for the identification of C. carrionii. J Clin Microbiol. 2004

Chromoblastomycosis adalah jamur yang menyerang kulit dan jaringan subkutan yang
ditandai dengan nodule verrucous atau plaque. Type konidia : Type cladosporium, type
Rhinocladiella dan type Phialophora Biopsi dengan pewarnaan H&E : Tampak adanya
bentukan sclerotic bodies yang berwarna coklat.

Jamur penyebab: Fonsecaea pedrosoi, Fonsecaea compacta, Phialophora verrucosa dan
Cladosporium carrionii. Chromoblastomycosis adalah jamur yang menyerang kulit dan
jaringan subkutan yang ditandai dengan nodule verrucous atau plaque.
Cladosporium carrionii, adalah agen dari chromoblastomycosis di daerah subtropis dan tropis
(tumbuh pada 35-37 C).

Spesies Cladosporium carrionii ditemukan menjadi heterogen.

Cladophialophora carrionii

Sinonim: Cladosporium carrionii

Cladophialophora carrionii adalah agen diakui chromoblastomycosis dan telah diisolasi dari posting
tanah dan pagar yang terbuat dari Eucalyptus spp. Kasus chromoblastomycosis disebabkan oleh C.
carrionii biasanya ditemukan di Australia, Venezuela, Madagaskar dan Amerika Selatan. Isolat dari
kista phaeomycotic dan infeksi oportunistik juga telah dilaporkan.

RG-2 organisme.

Morfologi Keterangan: Koloni yang tumbuh lambat, mencapai 3-4 cm dengan diameter setelah satu
bulan, dengan suede-seperti untuk berbulu halus permukaan kompak dan berwarna kuning langsat-
warna hitam. Mikroskop menunjukkan naik ke tegak, berwarna kuning langsat-hijau, apikal
bercabang, memanjang konidiofor memproduksi bercabang acropetal rantai konidia. Konidia yang
berwarna kuning langsat pucat, halus berdinding atau sedikit verrucose, limoniform untuk fusiform,
1,5-3,0 x 2,0-7,0 pM dalam ukuran. phialides bulat dengan collarettes besar dan menit, konidia hialin
kadang-kadang terbentuk pada media miskin gizi. Maksimum suhu pertumbuhan 35-37C.

Molekul Identifikasi: sequencing ITS dianjurkan (Abliz et al 2004; de Hoog et al 2007..).

Fitur Utama: Konidia lebih kecil dan terdiri rantai berat bercabang yang berantakan jauh lebih mudah
daripada di spesies lain Cladophialophora.

Referensi: McGinnis (1980), Rippon (1988), de Hoog et al. (1995, 2000, 2015).

Cladophialophora carrionii adalah salah satu agen penyebab relatif umum dari chromoblastomycosis.
Kami telah mengembangkan spesifik set oligonukleotida primer berdasarkan daerah spacer internal
yang ditranskrip dari DNA ribosom untuk identifikasi cepat patogen ini. PCR dengan set primer ini
diperkuat sebuah amplikon 362-bp dari C. carrionii strain. Dari spesies dematiaceous terkait lainnya,
termasuk jamur dematiaceous medis penting, seperti Fonsecaea pedrosoi, Phialophora verrucosa,
dan dermatitidis Exophiala, dan delapan spesies ragi medis penting, seperti Candida albicans dan
Cryptococcus neoformans var. neoformans, primer set tidak menghasilkan amplikon apapun. PCR
dengan primer set ini dapat menjadi alat yang berguna untuk identifikasi C. carrionii. J Clin Microbiol. 2004

Chromoblastomycosis Adalah jamur Yang Menyerang kulit Dan Jaringan subkutan Yang ditandai
DENGAN nodul verrucous ATAU plak. Ketik konidia: Type Cladosporium, jenis Rhinocladiella Dan Jenis
Phialophora Biopsi DENGAN pewarnaan H & E: Tampak adanya bentukan tubuh sklerotik Yang
berwarna coklat.

Jamur penyebab: Fonsecaea pedrosoi, Fonsecaea compacta, Phialophora verrucosa Dan

Cladosporium carrionii. Chromoblastomycosis Adalah jamur Yang Menyerang kulit Dan Jaringan
subkutan Yang ditandai DENGAN nodul verrucous ATAU plak.

Chromoblastomycosis is a chronic fungal infection of the skin and subcutaneous tissue. The infection
usually results from a traumatic injury and inoculation of microorganism from a specific group of
dematiaceous fungi (usuallyFonsecaea pedrosoi, Phialophora verrucosa, Cladophialophora carrionii).
In the tissues fungi produce characteristic sclerotic cells or muriform cells. Dermal lesions can range
from small nodules to large papillary-like eruptions. The disease has been described worldwide but
the prevalence is higher in rural populations in countries with a tropical or subtropical climate, such
as Madagascar in Africa and Brazil in South America. Diagnostic techniques are based on direct
examination, culture and histopathology. Despite a variety of treatment modalities, which include
long courses of antifungals, surgical excision and destructive physical therapies, the disease remains
one of the most difficult deep mycotic infections to eradicate. oleh PM Krzyciak - 2014


FUNGI (Cladosporium carrionii)

Khan, Abid Ali; Ahmad, Bashir; Lutfullah, Ghosia; Bacha, Nafees


October 2013


Pakistan Journal of Weed Science Research;2013, Vol. 19 Issue 4, p427


Academic Journal




Cladosporium carrionii were isolated from soil and were grown in Czapek yeast extract broth (CYB) for the
production of metabolites. The study was conducted in 2010 at Centre of Biotechnology and Microbiology,
University of Peshawar with the objectives to discover some biologically active secondary metabolites. The crude
extract and its fractions were tested against the pathogenic fungi namely Aspergillus flavus, Candida albicans,
Candida glabrata, Fusarium solani, Microsporum canis and Trichophyton longifusus, including brine shrimps and
Lemna aequinoctialis. The ethyl acetate extract showed highest inhibition of 66 mm against C. albicans, with MIC
of 0.25 mg/mL, n-Hexane fraction showed highest inhibition of 63 mm against C. glabrata, with MIC of 0.50
mg/mL and aqueous fraction showed highest inhibition of 56 mm against M. cannis, with MIC of 0.25 mg/mL. The
chemical investigation of the extract led to the isolation of pure antibacterial compounds and their structure was
confirmed by 1D and 2D spectroscopic techniques. The pure compounds [2-(4,6- dihydroxy-3-oxo-1,3-
dihydroisobenzofuran-1-yl) acetic acid (1) and 2- (4-hydroxy-1,3-dihydroisobenzofuran-1-yl) acetic acid (2)] were
isolated from C. carrionii and were tested only against the pathogenic bacteria, which showed growth inhibition
activities with minimum inhibitory concentration (MIC) value of 0.10 mg/mL for compound (1) and 0.50 mg/mL for
compound (2).