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RESULTS

Figure 1: Results of gel electrophoresis showing migration of unknown DNA sample A and B
on agarose gel. Lane 1 and 8 indicate the molecular weight (DNA ladder). Meanwhile, lane 2,
4 and 6 represent sample B whereas lane 3,5 and 7 display sample A.
Table 1: Data obtained through conducting the experiment for molecular weight (ladder). The
table shows the results of migrated distance (cm), the relative molecular weight and the log of
molecular weight of the respective bands for molecular weight (ladder).

Band Migrated Distance (cm) Molecular Weight Log Molecular Weight


1 2.00 10000 4.00
2 2.20 8000 3.90
3 2.30 6000 3.78
4 2.40 5000 3.70
5 2.60 4000 3.60
6 2.90 3000 3.48
7 3.20 2500 3.40
8 3.40 2000 3.30
9 3.80 1500 3.18
10 4.60 1000 3.00
11 6.10 750 2.88

Log Molecular Weight against Migrated Distance (cm)


4.5 4 3.9
3.78
3.73.6
4 3.48 3.43.3
3.5 3.18
3
Log Molecular Weight

2.88
3
2.5
2
y = -0.2773x + 4.3696
1.5 R = 0.881
1
0.5
0
0 1 2 3 4 5 6 7
Migrated Distance (cm)

Figure 2: The figure presents the line graph of the log molecular weight of DNA molecular
weight size marker against the migrated distance (cm) of the DNA molecular weight size
markers band. It is nearly linear for a range of molecular weights excluding very small and
very large molecular weight.
Table 2: The table shows the results of the migrated distance (cm), molecular weight and the
log molecular weight for the bands in sample B.

Bands Migrated Distance (cm) Log Molecular Weight Molecular Weight


1 1.70 3.90 7943
2 1.90 3.84 6918
3 2.10 3.79 6166
4 2.30 3.73 5370
5 2.60 3.65 4467
6 3.10 3.51 3236
7 3.30 3.45 2818

Calculation:

Given equation y = -0.2773x + 4.3696

x = Migrated Distance (cm)

y = Log Molecular Weight

Insert x = 1.70 cm into equation by substitution,

y = -0.2773(1.70) + 4.3696

y = 3.90

- log y = Molecular Weight

Molecular Weight = - log 3.90

Molecular Weight = 7943